RLG00000010652
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008124
Physical Location & Seq
Forward (+)
3347641 .. 3350014
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010652

Sequence Viewer

Length: 840 bp
ATGGGCGATGCCAAAGCGACGTCGTATTACCTCCCTCCCGGCTGCCGATTCTACCCCTCCGAGGAGCAGCTCCTCTGCTACTACCTCTCCCACAAGAACAGCGCCGGCCAGGCCAGCTCCGGCAACGGTGGCTATGATTTGATCAAGGAGCTCGACTTGTACGACCACGACCCGTTTGATTTGCCCGACTATGCCTGCTACTCGTACGGCCACGGCGGCCGGAGGCGGCACTGGTACTGCTACACGGTTAGGGTGGTGAAGGAGCGGCGGGCCAGGAGTGGGTATTGGAGGAGGAAAGGAAGGGTTAGGGACGTCGTGGGCCTCGGAGGGAAGGCGGTGTTGGGCCGGAGGAGTAGCTATGAGTTTTATTTGGGGAATTCTCCCAAGACGGCGGTGAGGACTGACTGGGTGCTCTATCAGTATGCTCAGGTTGATCACGTTCAGGCCTCTTTTGTCCTGTGCCGAGTATTTGTCAGATCTCAGGGTGGAACTAGCAAATCAGAGAATGGCTTAAGTTCTTGTGCTGAAGAAACTGTTTCCACAGTACGTCACATTGGAATTCAGCATGATGGATTTTGTAATCGTCATATTGTTCCTGAGATTCACGGTGACAAATCTGTGCCTAGGAACACTGACAAGTCAAAAGATCAAATGACACTGGAGACTGAAGTTGATAAGCAAGTCGAGACTGGGCCTTTATCAAGTATTCAGACTAATGAACAGGTGCCGTCATCTGTGCTCAGTGTTAGTAATCCATTGTTGCTTGATGGTTTGGCCACCGAGCATTTACTATCCCTTATAGAGGGAGACTTTATAGAGTTGGATGATCTCATCGACTGA

Protein Analysis

280

Amino Acids

31.34

Weight (kDa)

6.21

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 142 1.2e-21 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 23, 315
AccB1I GGYRCC 1 cut(s) 724
AccBSI CCGCTC 1 cut(s) 265
AciI CCGC 6 cut(s) 216, 226, 265, 268, 335, 392
AcoI YGGCCR 4 cut(s) 106, 208, 217, 774
AcsI RAATTY 2 cut(s) 376, 558
AcuI CTGAAG 2 cut(s) 546, 687
AcyI GRCGYC 2 cut(s) 20, 312
AfaI GTAC 4 cut(s) 161, 206, 236, 546
AfiI CCNNNNNNNGG 3 cut(s) 61, 279, 802
AflII CTTAAG 1 cut(s) 511
AjnI CCWGG 2 cut(s) 108, 272
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 4 cut(s) 70, 117, 151, 357
AluI AGCT 4 cut(s) 70, 117, 151, 357
Alw21I GWGCWC 3 cut(s) 153, 414, 741
Alw26I GTCTC 3 cut(s) 656, 680, 801
ApeKI GCWGC 2 cut(s) 42, 67
ApoI RAATTY 2 cut(s) 376, 558
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 4 cut(s) 270, 319, 343, 692
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 3 cut(s) 268, 406, 620
AvrII CCTAGG 1 cut(s) 623
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 3 cut(s) 153, 414, 741
BbvI GCAGC 2 cut(s) 29, 79
BccI CCATC 2 cut(s) 563, 761
BceAI ACGGC 4 cut(s) 223, 229, 405, 712
BciT130I CCWGG 2 cut(s) 110, 274
BclI TGATCA 2 cut(s) 141, 433
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 3 cut(s) 656, 680, 801
BfaI CTAG 2 cut(s) 492, 624
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 511
BglI GCCNNNNNGGC 2 cut(s) 110, 216
BglII AGATCT 1 cut(s) 476
BisI GCNGC 5 cut(s) 43, 68, 217, 227, 266
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 5 cut(s) 44, 69, 218, 228, 267
Bme1390I CCNGG 3 cut(s) 39, 110, 274
BmgT120I GGNCC 4 cut(s) 270, 319, 343, 692
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 3 cut(s) 39, 110, 274
BmrI ACTGGG 2 cut(s) 415, 699
BmuI ACTGGG 2 cut(s) 415, 699
BpmI CTGGAG 1 cut(s) 680
Bpu10I CCTNAGC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 39
BsaHI GRCGYC 2 cut(s) 20, 312
BsaJI CCNNGG 4 cut(s) 60, 211, 322, 623
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 279, 802
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 4 cut(s) 236, 410, 663, 694
BseBI CCWGG 2 cut(s) 110, 274
BseDI CCNNGG 4 cut(s) 60, 211, 322, 623
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 3 cut(s) 61, 279, 802
BseMII CTCAG 4 cut(s) 440, 494, 588, 754
BseNI ACTGG 4 cut(s) 236, 410, 663, 694
BseRI GAGGAG 4 cut(s) 62, 77, 304, 364
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 29, 79
Bsh1285I CGRYCG 1 cut(s) 220
BshNI GGYRCC 1 cut(s) 724
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 3 cut(s) 153, 414, 741
BsiSI CCGG 5 cut(s) 39, 105, 120, 220, 346
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 3 cut(s) 61, 279, 802
BsmAI GTCTC 3 cut(s) 656, 680, 801
BsmFI GGGAC 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 153, 414, 741
Bsp143I GATC 5 cut(s) 141, 433, 476, 646, 826
BspACI CCGC 6 cut(s) 216, 226, 265, 268, 335, 392
BspCNI CTCAG 4 cut(s) 439, 493, 589, 753
BspLI GGNNCC 1 cut(s) 726
BspT107I GGYRCC 1 cut(s) 724
BspTI CTTAAG 1 cut(s) 511
BsrBI CCGCTC 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 4 cut(s) 236, 410, 663, 694
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 60, 211, 322, 623
BssMI GATC 5 cut(s) 141, 433, 476, 646, 826
BssNI GRCGYC 2 cut(s) 20, 312
BssT1I CCWWGG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 110, 274
Bst4CI ACNGT 5 cut(s) 128, 247, 535, 544, 608
BstACI GRCGYC 2 cut(s) 20, 312
BstAFI CTTAAG 1 cut(s) 511
BstC8I GCNNGC 4 cut(s) 106, 115, 196, 270
BstDEI CTNAG 4 cut(s) 426, 480, 597, 740
BstDSI CCRYGG 1 cut(s) 211
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 1 cut(s) 829
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 5 cut(s) 144, 436, 479, 649, 829
BstMAI GTCTC 3 cut(s) 656, 680, 801
BstMBI GATC 5 cut(s) 141, 433, 476, 646, 826
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 4 cut(s) 110, 114, 129, 216
BstNI CCWGG 2 cut(s) 110, 274
BstSCI CCNGG 3 cut(s) 37, 108, 272
BstV1I GCAGC 2 cut(s) 29, 79
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BstZI CGGCCG 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 829
BtsIMutI CAGTG 4 cut(s) 229, 630, 656, 748
Cac8I GCNNGC 4 cut(s) 106, 115, 196, 270
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 4 cut(s) 270, 319, 343, 692
Csp6I GTAC 4 cut(s) 160, 205, 235, 545
CviAII CATG 1 cut(s) 566
CviQI GTAC 4 cut(s) 160, 205, 235, 545
DdeI CTNAG 4 cut(s) 426, 480, 597, 740
DpnI GATC 5 cut(s) 143, 435, 478, 648, 828
DpnII GATC 5 cut(s) 141, 433, 476, 646, 826
EaeI YGGCCR 4 cut(s) 106, 208, 217, 774
EagI CGGCCG 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 623
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 153
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 2 cut(s) 546, 687
EcoICRI GAGCTC 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoRI GAATTC 2 cut(s) 376, 558
EcoRII CCWGG 2 cut(s) 108, 272
EcoT14I CCWWGG 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 1 cut(s) 569
FaiI YATR 8 cut(s) 135, 192, 360, 423, 567, 588, 800, 815
FaqI GGGAC 1 cut(s) 323
FatI CATG 1 cut(s) 565
FauI CCCGC 1 cut(s) 261
FbaI TGATCA 2 cut(s) 141, 433
Fnu4HI GCNGC 5 cut(s) 43, 68, 217, 227, 266
FokI GGATG 1 cut(s) 836
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 5 cut(s) 43, 68, 217, 227, 266
FspBI CTAG 2 cut(s) 492, 624
GlaI GCGC 1 cut(s) 103
GluI GCNGC 5 cut(s) 43, 68, 217, 227, 266
GsuI CTGGAG 1 cut(s) 680
HaeII RGCGCY 1 cut(s) 105
HapII CCGG 5 cut(s) 39, 105, 120, 220, 346
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 2 cut(s) 20, 312
Hin1II CATG 1 cut(s) 569
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 2 cut(s) 48, 601
HpaII CCGG 5 cut(s) 39, 105, 120, 220, 346
HphI GGTGA 3 cut(s) 268, 406, 620
Hpy188I TCNGA 5 cut(s) 61, 326, 476, 502, 711
Hpy188III TCNNGA 2 cut(s) 596, 685
Hpy99I CGWCG 3 cut(s) 22, 25, 317
HpyAV CCTTC 3 cut(s) 253, 294, 325
HpyCH4III ACNGT 5 cut(s) 128, 247, 535, 544, 608
HpyCH4IV ACGT 4 cut(s) 20, 312, 438, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 114, 129, 216
HpyF3I CTNAG 4 cut(s) 426, 480, 597, 740
HpySE526I ACGT 4 cut(s) 20, 312, 438, 547
Hsp92I GRCGYC 2 cut(s) 20, 312
Hsp92II CATG 1 cut(s) 569
HspAI GCGC 1 cut(s) 102
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 2 cut(s) 141, 433
Kzo9I GATC 5 cut(s) 141, 433, 476, 646, 826
LmnI GCTCC 5 cut(s) 64, 75, 122, 148, 262
Lsp1109I GCAGC 2 cut(s) 29, 79
MaeI CTAG 2 cut(s) 492, 624
MaeII ACGT 4 cut(s) 20, 312, 438, 547
MaeIII GTNAC 2 cut(s) 548, 608
MalI GATC 5 cut(s) 143, 435, 478, 648, 828
MbiI CCGCTC 1 cut(s) 265
MboI GATC 5 cut(s) 141, 433, 476, 646, 826
MboII GAAGA 1 cut(s) 539
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 3 cut(s) 153, 414, 741
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 2 cut(s) 376, 558
MluNI TGGCCA 1 cut(s) 776
MmeI TCCRAC 1 cut(s) 801
Mox20I TGGCCA 1 cut(s) 776
MroNI GCCGGC 1 cut(s) 104
MscI TGGCCA 1 cut(s) 776
MseI TTAA 1 cut(s) 512
Msp20I TGGCCA 1 cut(s) 776
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 5 cut(s) 39, 105, 120, 220, 346
MspR9I CCNGG 3 cut(s) 39, 110, 274
MvaI CCWGG 2 cut(s) 110, 274
MwoI GCNNNNNNNGC 4 cut(s) 110, 114, 129, 216
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 1 cut(s) 39
NdeII GATC 5 cut(s) 141, 433, 476, 646, 826
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 569
NlaIV GGNNCC 1 cut(s) 726
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 2 cut(s) 548, 608
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 2 cut(s) 48, 601
Pfl23II CGTACG 1 cut(s) 204
PkrI GCNGC 5 cut(s) 44, 69, 218, 228, 267
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 108, 272
PspGI CCWGG 2 cut(s) 108, 272
PspLI CGTACG 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 4 cut(s) 270, 319, 343, 692
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 4 cut(s) 161, 206, 236, 546
RsaNI GTAC 4 cut(s) 160, 205, 235, 545
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 512
SatI GCNGC 5 cut(s) 43, 68, 217, 227, 266
Sau3AI GATC 5 cut(s) 141, 433, 476, 646, 826
Sau96I GGNCC 4 cut(s) 270, 319, 343, 692
ScrFI CCNGG 3 cut(s) 39, 110, 274
SduI GDGCHC 3 cut(s) 153, 414, 741
SfiI GGCCNNNNNGGCC 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 2 cut(s) 376, 558
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 6 cut(s) 216, 226, 265, 268, 335, 392
SspMI CTAG 2 cut(s) 492, 624
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 3 cut(s) 37, 108, 272
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 5 cut(s) 128, 247, 535, 544, 608
TaiI ACGT 4 cut(s) 23, 315, 441, 550
TaqI TCGA 3 cut(s) 153, 684, 834
TasI AATT 2 cut(s) 376, 558
TauI GCSGC 3 cut(s) 219, 229, 268
TfiI GAWTC 2 cut(s) 48, 601
Tru1I TTAA 1 cut(s) 512
Tru9I TTAA 1 cut(s) 512
TscAI CASTG 4 cut(s) 236, 637, 663, 748
TseFI GTSAC 2 cut(s) 548, 608
TseI GCWGC 2 cut(s) 42, 67
Tsp45I GTSAC 2 cut(s) 548, 608
TspDTI ATGAA 1 cut(s) 732
TspRI CASTG 4 cut(s) 236, 637, 663, 748
Vha464I CTTAAG 1 cut(s) 511
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 2 cut(s) 376, 558
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 2 cut(s) 492, 624
ZraI GACGTC 2 cut(s) 21, 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.