MD15G1079400.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008124
Physical Location & Seq
Forward (+)
5417906 .. 5420871
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1079400.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGGAGATCCTAAAGCGACGTCGTTGCTTTCGCTGGCTCCCGGTTGCCGATTCTACCCCTCGGAGGAGCAGCTCCTCTGCTACTACCTCTCCAGCAAGAACGCCGCCGAGGCGGGGAAATCGGGCGGCTATGATTTGATCAGAGAGCTCGATTTGTACGAACACGATCCCTTCGAGCTGCCGGAGAGCGCTTGCTATTCGTACGGCCACGGAGGGAGGAAGCGGCACTGGTTCTGCTACACGGTTAGGGTTTTGAAGGAGAGGAGGGCGAAGAGCGGGTATTGGCGGAGGAAAGGGAGGGTTAGGGACGTGGTAGGCCGCGGAGGGAAGGTGGTTCTGGGGAGGAGGTCGAGCTTTGTGTTTTATTTGGGGAATTCGCCAAACGACGCCGTAAGGACTGATTGGGTTCTCTATCAGTATGCTCAGGTCGATCATGTTAAGGCTGCTTTTGTCGTGTGCCGTATATTTGTCAGATCTGGTAGTGGAAATAGAATTTCAGATCATGGTTTAAGTTCTTGTGCTGAAGAAAGCGCTTGCACTGTTCGTCACATTGGAATTCAGCATGATGGATTTCATACTCCCAATATCAGTGAAAATGAAAAGCATGGTGACACTTCTGTGCCTAGGAAGAACGAGATGTCAAAATATCCATTGAGGCTGGACCGAGATCTTGATGATCGAGTCATGACAGGGCCTGTTTCTATATCAAGAATTCAGCCTAATGAACAGGTGCCAGCATCTGTGCTCGGTGGTAGTAATCCAATATTGCTTGATAATTTGGCAGCCAAGCAGTTACTTTCCATCATGGAGGGTGATTTTATAGAGTTGGATGATCTCATTGATTGA

Protein Analysis

282

Amino Acids

31.5

Weight (kDa)

7.63

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 14 - 141 2.5e-22 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 730
AccBSI CCGCTC 1 cut(s) 276
AccII CGCG 1 cut(s) 321
AciI CCGC 8 cut(s) 105, 113, 126, 223, 276, 286, 319, 321
AclWI GGATC 2 cut(s) 2, 161
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 4 cut(s) 373, 492, 555, 711
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 2 cut(s) 20, 387
AfaI GTAC 2 cut(s) 158, 203
AfeI AGCGCT 2 cut(s) 190, 532
AfiI CCNNNNNNNGG 2 cut(s) 64, 114
AgsI TTSAA 1 cut(s) 256
AjiI CACGTC 1 cut(s) 310
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 4 cut(s) 73, 148, 178, 354
AluI AGCT 4 cut(s) 73, 148, 178, 354
Alw21I GWGCWC 2 cut(s) 150, 747
AlwI GGATC 2 cut(s) 2, 161
AlwNI CAGNNNCTG 2 cut(s) 695, 740
Aor51HI AGCGCT 2 cut(s) 190, 532
AoxI GGCC 3 cut(s) 205, 316, 692
ApeKI GCWGC 4 cut(s) 70, 178, 443, 782
ApoI RAATTY 4 cut(s) 373, 492, 555, 711
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 2 cut(s) 191, 533
AspS9I GGNCC 2 cut(s) 661, 692
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 620, 824
AvaII GGWCC 1 cut(s) 661
AvrII CCTAGG 1 cut(s) 623
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 150, 747
BbvI GCAGC 4 cut(s) 82, 165, 430, 794
BccI CCATC 2 cut(s) 560, 809
BceAI ACGGC 3 cut(s) 220, 374, 444
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 42
BfaI CTAG 1 cut(s) 624
BfoI RGCGCY 2 cut(s) 192, 534
BglI GCCNNNNNGGC 1 cut(s) 110
BglII AGATCT 2 cut(s) 473, 667
BisI GCNGC 8 cut(s) 71, 105, 127, 179, 224, 319, 444, 783
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 8 cut(s) 72, 106, 128, 180, 225, 320, 445, 784
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 661
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 661, 692
BmiI GGNNCC 2 cut(s) 39, 732
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 42
BsaHI GRCGYC 2 cut(s) 20, 387
BsaJI CCNNGG 5 cut(s) 60, 108, 208, 319, 623
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 114
Bse1I ACTGG 1 cut(s) 233
BseDI CCNNGG 5 cut(s) 60, 108, 208, 319, 623
BseGI GGATG 1 cut(s) 835
BseLI CCNNNNNNNGG 2 cut(s) 64, 114
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 4 cut(s) 65, 80, 277, 358
BseXI GCAGC 4 cut(s) 82, 165, 430, 794
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 3 cut(s) 207, 318, 694
BshNI GGYRCC 1 cut(s) 730
BsiHKAI GWGCWC 2 cut(s) 150, 747
BsiSI CCGG 2 cut(s) 42, 182
BsiWI CGTACG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 64, 114
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 3 cut(s) 207, 318, 694
Bsp1286I GDGCHC 2 cut(s) 150, 747
Bsp143I GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
BspACI CCGC 8 cut(s) 105, 113, 126, 223, 276, 286, 319, 321
BspANI GGCC 3 cut(s) 207, 318, 694
BspCNI CTCAG 1 cut(s) 436
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 2 cut(s) 39, 732
BspPI GGATC 2 cut(s) 2, 161
BspQI GCTCTTC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 730
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 60, 108, 208, 319, 623
BssMI GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
BssNI GRCGYC 2 cut(s) 20, 387
BssT1I CCWWGG 1 cut(s) 623
Bst4CI ACNGT 2 cut(s) 244, 541
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 2 cut(s) 20, 387
BstC8I GCNNGC 4 cut(s) 36, 193, 535, 735
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 2 cut(s) 208, 319
BstF5I GGATG 1 cut(s) 835
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 2 cut(s) 192, 534
BstHHI GCGC 2 cut(s) 191, 533
BstKTI GATC 9 cut(s) 10, 141, 169, 433, 476, 502, 670, 679, 835
BstMBI GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 82, 165, 430, 794
BstX2I RGATCY 3 cut(s) 7, 473, 667
BstYI RGATCY 3 cut(s) 7, 473, 667
BsuRI GGCC 3 cut(s) 207, 318, 694
BtgI CCRYGG 2 cut(s) 208, 319
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 1 cut(s) 835
BtsIMutI CAGTG 3 cut(s) 226, 537, 595
Cac8I GCNNGC 4 cut(s) 36, 193, 535, 735
CaiI CAGNNNCTG 2 cut(s) 695, 740
CciI TCATGA 1 cut(s) 684
CfoI GCGC 2 cut(s) 191, 533
Cfr13I GGNCC 2 cut(s) 661, 692
Cfr42I CCGCGG 1 cut(s) 322
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 157, 202
CviAII CATG 6 cut(s) 434, 503, 563, 605, 685, 805
CviQI GTAC 2 cut(s) 157, 202
DdeI CTNAG 1 cut(s) 423
DpnI GATC 9 cut(s) 9, 140, 168, 432, 475, 501, 669, 678, 834
DpnII GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 623
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 661
Eco47III AGCGCT 2 cut(s) 190, 532
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 692
EcoRI GAATTC 3 cut(s) 373, 555, 711
EcoT14I CCWWGG 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 6 cut(s) 437, 506, 566, 608, 688, 808
FaqI GGGAC 1 cut(s) 320
FatI CATG 6 cut(s) 433, 502, 562, 604, 684, 804
FauI CCCGC 2 cut(s) 106, 269
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 71, 105, 127, 179, 224, 319, 444, 783
FokI GGATG 1 cut(s) 842
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 8 cut(s) 71, 105, 127, 179, 224, 319, 444, 783
FspBI CTAG 1 cut(s) 624
GlaI GCGC 2 cut(s) 190, 532
GluI GCNGC 8 cut(s) 71, 105, 127, 179, 224, 319, 444, 783
GsuI CTGGAG 1 cut(s) 76
HaeII RGCGCY 2 cut(s) 192, 534
HaeIII GGCC 3 cut(s) 207, 318, 694
HapII CCGG 2 cut(s) 42, 182
HgaI GACGC 1 cut(s) 395
HhaI GCGC 2 cut(s) 191, 533
Hin1I GRCGYC 2 cut(s) 20, 387
Hin1II CATG 6 cut(s) 437, 506, 566, 608, 688, 808
Hin6I GCGC 2 cut(s) 189, 531
HinP1I GCGC 2 cut(s) 189, 531
HinfI GANTC 2 cut(s) 51, 681
HpaII CCGG 2 cut(s) 42, 182
HphI GGTGA 2 cut(s) 620, 824
Hpy188I TCNGA 4 cut(s) 64, 143, 473, 499
Hpy188III TCNNGA 3 cut(s) 671, 685, 708
Hpy99I CGWCG 3 cut(s) 22, 25, 389
HpyAV CCTTC 3 cut(s) 181, 250, 322
HpyCH4III ACNGT 2 cut(s) 244, 541
HpyCH4IV ACGT 2 cut(s) 20, 309
HpyCH4V TGCA 1 cut(s) 537
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 2 cut(s) 20, 309
Hsp92I GRCGYC 2 cut(s) 20, 387
Hsp92II CATG 6 cut(s) 437, 506, 566, 608, 688, 808
HspAI GCGC 2 cut(s) 189, 531
Ksp22I TGATCA 1 cut(s) 138
KspI CCGCGG 1 cut(s) 322
Kzo9I GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 3 cut(s) 43, 67, 78
Lsp1109I GCAGC 4 cut(s) 82, 165, 430, 794
LweI GCATC 1 cut(s) 746
MaeI CTAG 1 cut(s) 624
MaeII ACGT 2 cut(s) 20, 309
MaeIII GTNAC 3 cut(s) 545, 608, 792
MalI GATC 9 cut(s) 9, 140, 168, 432, 475, 501, 669, 678, 834
MbiI CCGCTC 1 cut(s) 276
MboI GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
MboII GAAGA 3 cut(s) 283, 536, 640
MflI RGATCY 3 cut(s) 7, 473, 667
MhlI GDGCHC 2 cut(s) 150, 747
MluCI AATT 5 cut(s) 373, 492, 555, 711, 775
MlyI GAGTC 1 cut(s) 690
MmeI TCCRAC 1 cut(s) 807
MseI TTAA 2 cut(s) 438, 509
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 2 cut(s) 42, 182
MspR9I CCNGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 110
NciI CCSGG 1 cut(s) 42
NdeII GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
NlaIII CATG 6 cut(s) 437, 506, 566, 608, 688, 808
NlaIV GGNNCC 2 cut(s) 39, 732
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 545, 608
OliI CACNNNNGTG 1 cut(s) 617
PagI TCATGA 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 171
PfeI GAWTC 1 cut(s) 51
Pfl23II CGTACG 1 cut(s) 201
PkrI GCNGC 8 cut(s) 72, 106, 128, 180, 225, 320, 445, 784
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 150
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 39, 732
PspPI GGNCC 2 cut(s) 661, 692
PstNI CAGNNNCTG 2 cut(s) 695, 740
PsuI RGATCY 3 cut(s) 7, 473, 667
RsaI GTAC 2 cut(s) 158, 203
RsaNI GTAC 2 cut(s) 157, 202
RseI CAYNNNNRTG 1 cut(s) 617
SacI GAGCTC 1 cut(s) 150
SacII CCGCGG 1 cut(s) 322
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 2 cut(s) 438, 509
SatI GCNGC 8 cut(s) 71, 105, 127, 179, 224, 319, 444, 783
Sau3AI GATC 9 cut(s) 7, 138, 166, 430, 473, 499, 667, 676, 832
Sau96I GGNCC 2 cut(s) 661, 692
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 2 cut(s) 150, 747
SfaNI GCATC 1 cut(s) 746
Sfr303I CCGCGG 1 cut(s) 322
SgrBI CCGCGG 1 cut(s) 322
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 1 cut(s) 617
Sse9I AATT 5 cut(s) 373, 492, 555, 711, 775
SsiI CCGC 8 cut(s) 105, 113, 126, 223, 276, 286, 319, 321
SspI AATATT 1 cut(s) 765
SspMI CTAG 1 cut(s) 624
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 2 cut(s) 244, 541
TaiI ACGT 2 cut(s) 23, 312
TaqI TCGA 5 cut(s) 150, 174, 350, 429, 679
TaqII GACCGA 1 cut(s) 678
TasI AATT 5 cut(s) 373, 492, 555, 711, 775
TauI GCSGC 4 cut(s) 107, 129, 226, 321
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 2 cut(s) 438, 509
Tru9I TTAA 2 cut(s) 438, 509
TscAI CASTG 3 cut(s) 233, 544, 595
TseFI GTSAC 2 cut(s) 545, 608
TseI GCWGC 4 cut(s) 70, 178, 443, 782
Tsp45I GTSAC 2 cut(s) 545, 608
TspDTI ATGAA 3 cut(s) 563, 612, 738
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 3 cut(s) 233, 544, 595
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 4 cut(s) 373, 492, 555, 711
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 1 cut(s) 624
ZraI GACGTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.