Rw6G041400
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008124
Physical Location & Seq
Reverse (-)
65099880 .. 65103107
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041400.1

Sequence Viewer

Length: 666 bp
ATGGGCGATGCCAAAGCGACGTCGTATTACCTCCCTCCCGGTTGCGGATTCTACCCCTCCGAGGAGCAGCTCCTCTGCTACTACCTCTCCCACAAGAACGGCGCCGGCCAGGCCGGCTCCGGCAACGGTGGCTATGATTTGATCAAGGAGCTCGACTTGTACGACCGCGACCCCTTTGATTTGCCCGACTATGCCTGCTACTCGTACGGCCACGGCGGACGGAGGCGGCACTGGTACTGCTACACGGTTAGGGTTGTGAAGGAGCGGCGGGCCAAGAGCGGGTATTGGAGGAGGAAAGGAAGGGTTAGGGACGTCGTGGGCCGCGGAGGGAATGCCGTGTTGGGCCGGAGGAGTAGCTATGAGTTTTATTTGGGGAGTTCGCCCAAGACGGCGGTGAGGACTGACTGGGTGCTCTATCAGTATGCTCAGGTTGATCACGTTCAGGCCTCTTTTGTCCTGTGCCGAGTATTTGTCAGATCTCAGGGTGGAACTAGCAAATCAGAGAATGGCTTAAGTTCTTGTGCTGAAGAAACTGTTTCCACAGTACGTCACATTGGAATTCAGCATGGTGGATTTTGTACTCTCATATTGTTCCTGAGATTCACAGTGACAAATCTGTGCCTAGGAACACTGACAAGTCAAAGATCAAATGACACTGGAGACTGA

Protein Analysis

221

Amino Acids

24.74

Weight (kDa)

9.07

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 142 2.2e-20 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 23, 315
AccB1I GGYRCC 1 cut(s) 101
AccBSI CCGCTC 2 cut(s) 265, 279
AccII CGCG 2 cut(s) 168, 324
AcoI YGGCCR 2 cut(s) 106, 208
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 3 cut(s) 20, 102, 312
AfaI GTAC 5 cut(s) 161, 206, 236, 546, 580
AfiI CCNNNNNNNGG 3 cut(s) 44, 61, 279
AflII CTTAAG 1 cut(s) 511
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 3 cut(s) 70, 151, 357
AluI AGCT 3 cut(s) 70, 151, 357
Alw21I GWGCWC 2 cut(s) 153, 414
Alw26I GTCTC 1 cut(s) 654
AoxI GGCC 7 cut(s) 106, 111, 208, 270, 319, 343, 444
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 558
AspA2I CCTAGG 1 cut(s) 622
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 3 cut(s) 270, 319, 343
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 406
AvrII CCTAGG 1 cut(s) 622
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 153, 414
BbvI GCAGC 1 cut(s) 79
BceAI ACGGC 5 cut(s) 115, 223, 229, 320, 405
BciT130I CCWGG 1 cut(s) 110
BclI TGATCA 2 cut(s) 141, 433
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 654
BfaI CTAG 2 cut(s) 492, 623
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 511
BglI GCCNNNNNGGC 2 cut(s) 110, 114
BglII AGATCT 1 cut(s) 476
BisI GCNGC 4 cut(s) 68, 227, 266, 322
BlnI CCTAGG 1 cut(s) 622
BlsI GCNGC 4 cut(s) 69, 228, 267, 323
Bme1390I CCNGG 2 cut(s) 39, 110
BmgT120I GGNCC 3 cut(s) 270, 319, 343
BmiI GGNNCC 2 cut(s) 103, 118
BmrFI CCNGG 2 cut(s) 39, 110
BmrI ACTGGG 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 39
BsaHI GRCGYC 3 cut(s) 20, 102, 312
BsaJI CCNNGG 4 cut(s) 60, 211, 322, 622
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 61, 279
Bse118I RCCGGY 2 cut(s) 104, 113
Bse1I ACTGG 3 cut(s) 236, 410, 661
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 4 cut(s) 60, 211, 322, 622
BseLI CCNNNNNNNGG 3 cut(s) 44, 61, 279
BseMII CTCAG 3 cut(s) 440, 494, 587
BseNI ACTGG 3 cut(s) 236, 410, 661
BseRI GAGGAG 4 cut(s) 62, 77, 304, 364
BseXI GCAGC 1 cut(s) 79
Bsh1236I CGCG 2 cut(s) 168, 324
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 7 cut(s) 108, 113, 210, 272, 321, 345, 446
BshNI GGYRCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 2 cut(s) 153, 414
BsiSI CCGG 5 cut(s) 39, 105, 114, 120, 346
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 3 cut(s) 44, 61, 279
BsmAI GTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 7 cut(s) 108, 113, 210, 272, 321, 345, 446
Bsp1286I GDGCHC 2 cut(s) 153, 414
Bsp143I GATC 4 cut(s) 141, 433, 476, 644
BspANI GGCC 7 cut(s) 108, 113, 210, 272, 321, 345, 446
BspCNI CTCAG 3 cut(s) 439, 493, 588
BspFNI CGCG 2 cut(s) 168, 324
BspLI GGNNCC 2 cut(s) 103, 118
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 1 cut(s) 511
BsrBI CCGCTC 2 cut(s) 265, 279
BsrFI RCCGGY 2 cut(s) 104, 113
BsrI ACTGG 3 cut(s) 236, 410, 661
BssAI RCCGGY 2 cut(s) 104, 113
BssECI CCNNGG 4 cut(s) 60, 211, 322, 622
BssMI GATC 4 cut(s) 141, 433, 476, 644
BssNI GRCGYC 3 cut(s) 20, 102, 312
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 5 cut(s) 128, 247, 535, 544, 607
BstACI GRCGYC 3 cut(s) 20, 102, 312
BstAFI CTTAAG 1 cut(s) 511
BstC8I GCNNGC 4 cut(s) 106, 115, 196, 270
BstDEI CTNAG 3 cut(s) 426, 480, 596
BstDSI CCRYGG 2 cut(s) 211, 322
BstFNI CGCG 2 cut(s) 168, 324
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 4 cut(s) 144, 436, 479, 647
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 4 cut(s) 141, 433, 476, 644
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 110, 114, 129
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 2 cut(s) 37, 108
BstUI CGCG 2 cut(s) 168, 324
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuRI GGCC 7 cut(s) 108, 113, 210, 272, 321, 345, 446
BtgI CCRYGG 2 cut(s) 211, 322
BtgZI GCGATG 1 cut(s) 21
BtsIMutI CAGTG 4 cut(s) 229, 612, 629, 654
Cac8I GCNNGC 4 cut(s) 106, 115, 196, 270
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 2 cut(s) 104, 113
Cfr13I GGNCC 3 cut(s) 270, 319, 343
Cfr42I CCGCGG 1 cut(s) 325
Csp6I GTAC 5 cut(s) 160, 205, 235, 545, 579
CviAII CATG 1 cut(s) 566
CviQI GTAC 5 cut(s) 160, 205, 235, 545, 579
DdeI CTNAG 3 cut(s) 426, 480, 596
DinI GGCGCC 1 cut(s) 103
DpnI GATC 4 cut(s) 143, 435, 478, 646
DpnII GATC 4 cut(s) 141, 433, 476, 644
EaeI YGGCCR 2 cut(s) 106, 208
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 622
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 622
EcoT38I GRGCYC 1 cut(s) 153
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 1 cut(s) 569
FaiI YATR 6 cut(s) 135, 192, 360, 423, 567, 587
FaqI GGGAC 1 cut(s) 323
FatI CATG 1 cut(s) 565
FauI CCCGC 2 cut(s) 261, 272
FbaI TGATCA 2 cut(s) 141, 433
Fnu4HI GCNGC 4 cut(s) 68, 227, 266, 322
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 4 cut(s) 68, 227, 266, 322
FspBI CTAG 2 cut(s) 492, 623
GlaI GCGC 1 cut(s) 103
GluI GCNGC 4 cut(s) 68, 227, 266, 322
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 7 cut(s) 108, 113, 210, 272, 321, 345, 446
HapII CCGG 5 cut(s) 39, 105, 114, 120, 346
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 3 cut(s) 20, 102, 312
Hin1II CATG 1 cut(s) 569
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 2 cut(s) 48, 600
HpaII CCGG 5 cut(s) 39, 105, 114, 120, 346
HphI GGTGA 1 cut(s) 406
Hpy188I TCNGA 3 cut(s) 61, 476, 502
Hpy188III TCNNGA 1 cut(s) 595
Hpy99I CGWCG 3 cut(s) 22, 25, 317
HpyAV CCTTC 2 cut(s) 253, 294
HpyCH4III ACNGT 5 cut(s) 128, 247, 535, 544, 607
HpyCH4IV ACGT 4 cut(s) 20, 312, 438, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 114, 129
HpyF3I CTNAG 3 cut(s) 426, 480, 596
HpySE526I ACGT 4 cut(s) 20, 312, 438, 547
Hsp92I GRCGYC 3 cut(s) 20, 102, 312
Hsp92II CATG 1 cut(s) 569
HspAI GCGC 1 cut(s) 102
KasI GGCGCC 1 cut(s) 101
KroI GCCGGC 2 cut(s) 104, 113
KroNI GCCGGC 2 cut(s) 106, 115
Ksp22I TGATCA 2 cut(s) 141, 433
KspI CCGCGG 1 cut(s) 325
Kzo9I GATC 4 cut(s) 141, 433, 476, 644
LmnI GCTCC 5 cut(s) 64, 75, 122, 148, 262
Lsp1109I GCAGC 1 cut(s) 79
MaeI CTAG 2 cut(s) 492, 623
MaeII ACGT 4 cut(s) 20, 312, 438, 547
MaeIII GTNAC 2 cut(s) 548, 607
MalI GATC 4 cut(s) 143, 435, 478, 646
MbiI CCGCTC 2 cut(s) 265, 279
MboI GATC 4 cut(s) 141, 433, 476, 644
MboII GAAGA 1 cut(s) 539
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 2 cut(s) 153, 414
MluCI AATT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 102
MroNI GCCGGC 2 cut(s) 104, 113
MseI TTAA 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 324
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 5 cut(s) 39, 105, 114, 120, 346
MspR9I CCNGG 2 cut(s) 39, 110
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 2 cut(s) 168, 324
MwoI GCNNNNNNNGC 3 cut(s) 110, 114, 129
NaeI GCCGGC 2 cut(s) 106, 115
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 1 cut(s) 39
NdeII GATC 4 cut(s) 141, 433, 476, 644
NgoMIV GCCGGC 2 cut(s) 104, 113
NlaIII CATG 1 cut(s) 569
NlaIV GGNNCC 2 cut(s) 103, 118
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 2 cut(s) 548, 607
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 337
PdiI GCCGGC 2 cut(s) 106, 115
PfeI GAWTC 2 cut(s) 48, 600
Pfl23II CGTACG 1 cut(s) 204
PkrI GCNGC 4 cut(s) 69, 228, 267, 323
PluTI GGCGCC 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspLI CGTACG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 103, 118
PspPI GGNCC 3 cut(s) 270, 319, 343
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 5 cut(s) 161, 206, 236, 546, 580
RsaNI GTAC 5 cut(s) 160, 205, 235, 545, 579
SacI GAGCTC 1 cut(s) 153
SacII CCGCGG 1 cut(s) 325
SaqAI TTAA 1 cut(s) 512
SatI GCNGC 4 cut(s) 68, 227, 266, 322
Sau3AI GATC 4 cut(s) 141, 433, 476, 644
Sau96I GGNCC 3 cut(s) 270, 319, 343
ScrFI CCNGG 2 cut(s) 39, 110
SduI GDGCHC 2 cut(s) 153, 414
SfoI GGCGCC 1 cut(s) 103
Sfr303I CCGCGG 1 cut(s) 325
SgrBI CCGCGG 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 1 cut(s) 558
SseBI AGGCCT 1 cut(s) 446
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 492, 623
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 37, 108
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 5 cut(s) 128, 247, 535, 544, 607
TaiI ACGT 4 cut(s) 23, 315, 441, 550
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 558
TatI WGTACW 1 cut(s) 578
TauI GCSGC 3 cut(s) 229, 268, 324
TfiI GAWTC 2 cut(s) 48, 600
Tru1I TTAA 1 cut(s) 512
Tru9I TTAA 1 cut(s) 512
TscAI CASTG 4 cut(s) 236, 612, 636, 661
TseFI GTSAC 2 cut(s) 548, 607
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 2 cut(s) 548, 607
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 4 cut(s) 236, 612, 636, 661
Vha464I CTTAAG 1 cut(s) 511
XapI RAATTY 1 cut(s) 558
XmaJI CCTAGG 1 cut(s) 622
XspI CTAG 2 cut(s) 492, 623
ZraI GACGTC 2 cut(s) 21, 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.