MD08G1096700.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008124
Physical Location & Seq
Forward (+)
8123606 .. 8126314
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1096700.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGGAGATCCTAAAGCGACGTCGTTGCTTTCGCTGGCCCCCGGTTGCCGATTCTACCCCTCGGAGGAGCAGCTCCTCTGCTACTACCTCTCCAGCAAGAACGCCGCCGAGGCGGGGAAATCGGGCGGGTACGATTTGATCAGGGAGCTCGATTTGTACGGCCACGATCCGTTCGATTTGCCGGAGAGCGCATGTTACTCGTATGGCCACGGAGGGAGGAAGAGGCACTGGTTCTGCTACACGGTTAGGATCTTGAAGAAGAGGAGGGCGAAGAGCGGGTATTGGCGGAGGCAAGGGCGGGTTAGGGACGTGGTGGGCCGCGGAGGGACGGTGGTTCTGGGGAGGAGGTCGAGCTTTGTGTTTTATTTGGGGAATTCGCCAAACGACGCCGTAAGGACTGATTTGGTTCTCCATCAGTATGCTCAGGTCGATCATGTTAAGGCTGCTTTTGTCCTTTGCCGTATTTTGGTGAGATCTCGTAGTGGAAATAGAATATCAGATCATGGTTTAAGTTCTTATGCTGAACAAAGTGCTTGCACTGTTCGTCACATTGGAATTCAGCATGATGGATTTCATACTCCCAGTAGTAGTGAAAATGAAGTGCATGGTGACACTTCTGTGCCTAGGAAAAATGAGATGTCAAAATATCCGAGGCTGGACCCTGAGCTAGATGATCGAGTCATGACAAGGCCTGTTTCTATATCAAGGATTCAGTCTAATGAACAGGTGCCAGCATCTGTGCTCGGTGGTAGGAATCCAATATTGCTTGATAATTTGGGGGCCAAGCACTTACATTCCATTATGGAGGGTGATTTTATAGAGTTGGATGATCTCATCGATTGA

Protein Analysis

281

Amino Acids

31.38

Weight (kDa)

8.68

Isoelectric Point (pI)

58.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 14 - 141 7.9e-21 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 727
AccBSI CCGCTC 1 cut(s) 276
AccII CGCG 1 cut(s) 321
AciI CCGC 8 cut(s) 105, 113, 126, 276, 286, 298, 319, 321
AclWI GGATC 3 cut(s) 2, 161, 257
AcoI YGGCCR 2 cut(s) 160, 205
AcsI RAATTY 2 cut(s) 373, 555
AcyI GRCGYC 2 cut(s) 20, 387
AfaI GTAC 2 cut(s) 131, 158
AfiI CCNNNNNNNGG 3 cut(s) 64, 114, 466
AgsI TTSAA 1 cut(s) 256
AjiI CACGTC 1 cut(s) 310
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 4 cut(s) 73, 148, 354, 667
AluI AGCT 4 cut(s) 73, 148, 354, 667
Alw21I GWGCWC 2 cut(s) 150, 744
AlwI GGATC 3 cut(s) 2, 161, 257
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 6 cut(s) 36, 160, 205, 316, 689, 780
ApeKI GCWGC 2 cut(s) 70, 443
ApoI RAATTY 2 cut(s) 373, 555
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 4 cut(s) 37, 316, 658, 780
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 481, 620, 821
AvaII GGWCC 1 cut(s) 658
AvrII CCTAGG 1 cut(s) 623
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 150, 744
BbvI GCAGC 2 cut(s) 82, 430
BccI CCATC 2 cut(s) 420, 560
BceAI ACGGC 3 cut(s) 175, 374, 444
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 180, 214
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 42
BfaI CTAG 2 cut(s) 624, 668
BglI GCCNNNNNGGC 1 cut(s) 110
BglII AGATCT 1 cut(s) 473
BisI GCNGC 4 cut(s) 71, 105, 319, 444
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 4 cut(s) 72, 106, 320, 445
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 658
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 4 cut(s) 37, 316, 658, 780
BmiI GGNNCC 4 cut(s) 39, 660, 729, 781
BmrFI CCNGG 1 cut(s) 42
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 576
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 2 cut(s) 423, 663
BpuMI CCSGG 1 cut(s) 42
Bsa29I ATCGAT 1 cut(s) 837
BsaHI GRCGYC 2 cut(s) 20, 387
BsaJI CCNNGG 7 cut(s) 40, 60, 108, 208, 319, 623, 650
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 315, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 114, 466
Bse1I ACTGG 2 cut(s) 233, 582
BseCI ATCGAT 1 cut(s) 837
BseDI CCNNGG 7 cut(s) 40, 60, 108, 208, 319, 623, 650
BseGI GGATG 1 cut(s) 832
BseLI CCNNNNNNNGG 3 cut(s) 64, 114, 466
BseMII CTCAG 2 cut(s) 437, 654
BseNI ACTGG 2 cut(s) 233, 582
BseRI GAGGAG 4 cut(s) 65, 80, 277, 358
BseXI GCAGC 2 cut(s) 82, 430
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 6 cut(s) 38, 162, 207, 318, 691, 782
BshNI GGYRCC 1 cut(s) 727
BshVI ATCGAT 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 150, 744
BsiSI CCGG 2 cut(s) 42, 182
BslFI GGGAC 2 cut(s) 320, 340
BslI CCNNNNNNNGG 3 cut(s) 64, 114, 466
BsmFI GGGAC 2 cut(s) 320, 340
BsnI GGCC 6 cut(s) 38, 162, 207, 318, 691, 782
Bsp1286I GDGCHC 2 cut(s) 150, 744
Bsp143I GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
BspACI CCGC 8 cut(s) 105, 113, 126, 276, 286, 298, 319, 321
BspANI GGCC 6 cut(s) 38, 162, 207, 318, 691, 782
BspCNI CTCAG 2 cut(s) 436, 655
BspDI ATCGAT 1 cut(s) 837
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 4 cut(s) 39, 660, 729, 781
BspPI GGATC 3 cut(s) 2, 161, 257
BspQI GCTCTTC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 727
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 2 cut(s) 233, 582
BssECI CCNNGG 7 cut(s) 40, 60, 108, 208, 319, 623, 650
BssMI GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
BssNI GRCGYC 2 cut(s) 20, 387
BssT1I CCWWGG 1 cut(s) 623
Bst4CI ACNGT 3 cut(s) 244, 331, 541
Bst6I CTCTTC 3 cut(s) 215, 254, 266
BstACI GRCGYC 2 cut(s) 20, 387
BstC8I GCNNGC 3 cut(s) 36, 535, 732
BstDEI CTNAG 2 cut(s) 423, 663
BstDSI CCRYGG 2 cut(s) 208, 319
BstF5I GGATG 1 cut(s) 832
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 9 cut(s) 10, 141, 169, 252, 433, 476, 502, 676, 832
BstMBI GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 82, 430
BstX2I RGATCY 3 cut(s) 7, 249, 473
BstYI RGATCY 3 cut(s) 7, 249, 473
Bsu15I ATCGAT 1 cut(s) 837
BsuRI GGCC 6 cut(s) 38, 162, 207, 318, 691, 782
BsuTUI ATCGAT 1 cut(s) 837
BtgI CCRYGG 2 cut(s) 208, 319
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 1 cut(s) 832
BtsIMutI CAGTG 2 cut(s) 226, 537
Cac8I GCNNGC 3 cut(s) 36, 535, 732
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 681
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 4 cut(s) 37, 316, 658, 780
Cfr42I CCGCGG 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 837
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 130, 157
CviAII CATG 6 cut(s) 192, 434, 503, 563, 605, 682
CviQI GTAC 2 cut(s) 130, 157
DdeI CTNAG 2 cut(s) 423, 663
DpnI GATC 9 cut(s) 9, 140, 168, 251, 432, 475, 501, 675, 831
DpnII GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
EaeI YGGCCR 2 cut(s) 160, 205
Eam1104I CTCTTC 3 cut(s) 215, 254, 266
EarI CTCTTC 3 cut(s) 215, 254, 266
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 623
Eco147I AGGCCT 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 658
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoRI GAATTC 2 cut(s) 373, 555
EcoT14I CCWWGG 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 6 cut(s) 195, 437, 506, 566, 608, 685
FaqI GGGAC 2 cut(s) 320, 340
FatI CATG 6 cut(s) 191, 433, 502, 562, 604, 681
FauI CCCGC 4 cut(s) 106, 119, 269, 291
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 71, 105, 319, 444
FokI GGATG 1 cut(s) 839
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 71, 105, 319, 444
FspBI CTAG 2 cut(s) 624, 668
GlaI GCGC 1 cut(s) 190
GluI GCNGC 4 cut(s) 71, 105, 319, 444
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 6 cut(s) 38, 162, 207, 318, 691, 782
HapII CCGG 2 cut(s) 42, 182
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 2 cut(s) 20, 387
Hin1II CATG 6 cut(s) 195, 437, 506, 566, 608, 685
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 4 cut(s) 51, 678, 709, 754
HpaII CCGG 2 cut(s) 42, 182
HphI GGTGA 3 cut(s) 481, 620, 821
Hpy188I TCNGA 3 cut(s) 64, 499, 651
Hpy188III TCNNGA 2 cut(s) 253, 682
Hpy99I CGWCG 3 cut(s) 22, 25, 389
HpyCH4III ACNGT 3 cut(s) 244, 331, 541
HpyCH4IV ACGT 2 cut(s) 20, 309
HpyCH4V TGCA 2 cut(s) 537, 604
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 423, 663
HpySE526I ACGT 2 cut(s) 20, 309
Hsp92I GRCGYC 2 cut(s) 20, 387
Hsp92II CATG 6 cut(s) 195, 437, 506, 566, 608, 685
HspAI GCGC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 138
KspI CCGCGG 1 cut(s) 322
Kzo9I GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 3 cut(s) 67, 78, 145
Lsp1109I GCAGC 2 cut(s) 82, 430
LweI GCATC 1 cut(s) 743
MaeI CTAG 2 cut(s) 624, 668
MaeII ACGT 2 cut(s) 20, 309
MaeIII GTNAC 3 cut(s) 194, 545, 608
MalI GATC 9 cut(s) 9, 140, 168, 251, 432, 475, 501, 675, 831
MbiI CCGCTC 1 cut(s) 276
MboI GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
MboII GAAGA 4 cut(s) 232, 268, 271, 283
MflI RGATCY 3 cut(s) 7, 249, 473
MhlI GDGCHC 2 cut(s) 150, 744
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 3 cut(s) 373, 555, 772
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 804
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 2 cut(s) 438, 509
MslI CAYNNNNRTG 2 cut(s) 417, 617
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 2 cut(s) 42, 182
MspR9I CCNGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 110
NciI CCSGG 1 cut(s) 42
NdeII GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
NlaIII CATG 6 cut(s) 195, 437, 506, 566, 608, 685
NlaIV GGNNCC 4 cut(s) 39, 660, 729, 781
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 545, 608
NspI RCATGY 1 cut(s) 195
OliI CACNNNNGTG 1 cut(s) 617
PagI TCATGA 1 cut(s) 681
PceI AGGCCT 1 cut(s) 691
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 3 cut(s) 51, 709, 754
PkrI GCNGC 4 cut(s) 72, 106, 320, 445
PleI GAGTC 1 cut(s) 686
PpsI GAGTC 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 39, 660, 729, 781
PspPI GGNCC 4 cut(s) 37, 316, 658, 780
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 3 cut(s) 7, 249, 473
RsaI GTAC 2 cut(s) 131, 158
RsaNI GTAC 2 cut(s) 130, 157
RseI CAYNNNNRTG 2 cut(s) 417, 617
SacI GAGCTC 1 cut(s) 150
SacII CCGCGG 1 cut(s) 322
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 2 cut(s) 438, 509
SatI GCNGC 4 cut(s) 71, 105, 319, 444
Sau3AI GATC 9 cut(s) 7, 138, 166, 249, 430, 473, 499, 673, 829
Sau96I GGNCC 4 cut(s) 37, 316, 658, 780
SchI GAGTC 1 cut(s) 687
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 2 cut(s) 150, 744
SfaNI GCATC 1 cut(s) 743
Sfr303I CCGCGG 1 cut(s) 322
SgrBI CCGCGG 1 cut(s) 322
SinI GGWCC 1 cut(s) 658
SmiMI CAYNNNNRTG 2 cut(s) 417, 617
Sse9I AATT 3 cut(s) 373, 555, 772
SseBI AGGCCT 1 cut(s) 691
SsiI CCGC 8 cut(s) 105, 113, 126, 276, 286, 298, 319, 321
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 624, 668
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 691
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 3 cut(s) 244, 331, 541
TaiI ACGT 2 cut(s) 23, 312
TaqI TCGA 6 cut(s) 150, 174, 350, 429, 676, 837
TasI AATT 3 cut(s) 373, 555, 772
TauI GCSGC 2 cut(s) 107, 321
TfiI GAWTC 3 cut(s) 51, 709, 754
Tru1I TTAA 2 cut(s) 438, 509
Tru9I TTAA 2 cut(s) 438, 509
TscAI CASTG 2 cut(s) 233, 544
TseFI GTSAC 2 cut(s) 545, 608
TseI GCWGC 2 cut(s) 70, 443
Tsp45I GTSAC 2 cut(s) 545, 608
TspDTI ATGAA 3 cut(s) 563, 612, 735
TspGWI ACGGA 2 cut(s) 159, 225
TspRI CASTG 2 cut(s) 233, 544
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 2 cut(s) 373, 555
XceI RCATGY 1 cut(s) 195
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 2 cut(s) 624, 668
ZraI GACGTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.