Rh4CG109900

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008124
Physical Location & Seq
Reverse (-)
20618467 .. 20629808
11342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG109900.1

Sequence Viewer

Length: 660 bp
ATGACTCGTTGTCAAAGGTCAGTACTGGTGCACATCTTGCAGGCAAATGCTTTCGAATTGCTAAAATGTTCTGTTGGGAACCACGTACAAAGATTGGAACAGCAGCTGCTTGAAGAAATAGAGCTACGTGCTATTCTGGAAAATGCAATTGAGAAAAATGCTACTAAATTCTCCAGTCCCGCATGCCTTCCTTACAACGCTCAAGAGCTCCTAAGCATTATTGCTGCAGTGGAGGTGACAGTTTCAGAACTAGAACAGGAGTTGGTTGCATTGCATTTCCAACCAAGTCAAGAAAGAAATGAAAGAAGGCTGGCAGAATATCGTCTGAGGCATCCATCTCCTCGGTCAATGTCTCCTCGCCCGTCTAGCATTATGAAACTGCCGGCCTCTTTTGTCCTGTGCCGAGTATTTGTCAGATCTCAAGGTGGAACTACCAAATCAGAGAATGGCTTAAGTTCTTGTGCTGAAGAAACTGTTTCCACAGTATGTCACATTGGAATTCAGCATGGTGGATTTTGTACTCCTCATATTGTTCCTGAGATTCACAGTGACAAATCTGTGCCTAGGAACACTGACAAGTCAAAAGATCAAATGACACTGGAGACTGAAGTTGATAAGCAAGTCGATCGAGACTGGGCCTCTACCAAGTATTCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

219

Amino Acids

24.63

Weight (kDa)

6.25

Isoelectric Point (pI)

69.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 26 - 98 5.3e-15 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 180
AcsI RAATTY 2 cut(s) 167, 498
AcuI CTGAAG 2 cut(s) 486, 627
AfaI GTAC 3 cut(s) 24, 87, 520
AflII CTTAAG 1 cut(s) 451
AgsI TTSAA 1 cut(s) 113
AhdI GACNNNNNGTC 1 cut(s) 9
AluBI AGCT 3 cut(s) 106, 124, 208
AluI AGCT 3 cut(s) 106, 124, 208
Alw21I GWGCWC 2 cut(s) 33, 210
Alw26I GTCTC 3 cut(s) 357, 596, 624
Alw44I GTGCAC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 384, 636
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 3 cut(s) 103, 106, 224
ApoI RAATTY 2 cut(s) 167, 498
AspA2I CCTAGG 1 cut(s) 563
AspS9I GGNCC 1 cut(s) 636
AsuHPI GGTGA 1 cut(s) 247
AsuII TTCGAA 1 cut(s) 54
AvrII CCTAGG 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 2 cut(s) 33, 210
BbvI GCAGC 3 cut(s) 93, 115, 211
BccI CCATC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BcoDI GTCTC 3 cut(s) 357, 596, 624
BfaI CTAG 3 cut(s) 251, 366, 564
BfmI CTRYAG 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 451
BglII AGATCT 1 cut(s) 416
BisI GCNGC 3 cut(s) 104, 107, 225
BlnI CCTAGG 1 cut(s) 563
BlsI GCNGC 3 cut(s) 105, 108, 226
BmcAI AGTACT 1 cut(s) 24
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 636
BmiI GGNNCC 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 643
BpmI CTGGAG 2 cut(s) 157, 620
Bpu10I CCTNAGC 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 186, 405
BsaAI YACGTR 2 cut(s) 85, 128
BsaJI CCNNGG 2 cut(s) 341, 563
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 4 cut(s) 30, 174, 603, 638
Bse3DI GCAATG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 341, 563
BseGI GGATG 1 cut(s) 331
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 317, 528
BseNI ACTGG 4 cut(s) 30, 174, 603, 638
BseRI GAGGAG 3 cut(s) 330, 345, 513
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 3 cut(s) 93, 115, 211
Bsh1285I CGRYCG 1 cut(s) 628
BshFI GGCC 2 cut(s) 386, 638
BsiEI CGRYCG 1 cut(s) 628
BsiHKAI GWGCWC 2 cut(s) 33, 210
BsiSI CCGG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 162
BsmAI GTCTC 3 cut(s) 357, 596, 624
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 2 cut(s) 386, 638
Bsp119I TTCGAA 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 33, 210
Bsp143I GATC 3 cut(s) 416, 586, 625
BspACI CCGC 1 cut(s) 180
BspANI GGCC 2 cut(s) 386, 638
BspCNI CTCAG 2 cut(s) 318, 529
BspLI GGNNCC 1 cut(s) 80
BspMAI CTGCAG 1 cut(s) 229
BspT104I TTCGAA 1 cut(s) 54
BspTI CTTAAG 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 4 cut(s) 30, 174, 603, 638
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 341, 563
BssMI GATC 3 cut(s) 416, 586, 625
BssT1I CCWWGG 1 cut(s) 563
Bst4CI ACNGT 4 cut(s) 241, 475, 484, 548
BstAFI CTTAAG 1 cut(s) 451
BstAPI GCANNNNNTGC 1 cut(s) 37
BstBAI YACGTR 2 cut(s) 85, 128
BstBI TTCGAA 1 cut(s) 54
BstC8I GCNNGC 4 cut(s) 42, 184, 312, 384
BstDEI CTNAG 3 cut(s) 212, 326, 537
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 3 cut(s) 419, 589, 628
BstMAI GTCTC 3 cut(s) 357, 596, 624
BstMBI GATC 3 cut(s) 416, 586, 625
BstMCI CGRYCG 1 cut(s) 628
BstMWI GCNNNNNNNGC 2 cut(s) 37, 366
BstNSI RCATGY 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 93, 115, 211
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 2 cut(s) 386, 638
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 4 cut(s) 234, 553, 570, 596
Cac8I GCNNGC 4 cut(s) 42, 184, 312, 384
CaiI CAGNNNCTG 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 636
Csp6I GTAC 3 cut(s) 23, 86, 519
CviAII CATG 2 cut(s) 183, 506
CviJI RGCY 7 cut(s) 106, 124, 208, 310, 386, 450, 638
CviKI_1 RGCY 7 cut(s) 106, 124, 208, 310, 386, 450, 638
CviQI GTAC 3 cut(s) 23, 86, 519
DdeI CTNAG 3 cut(s) 212, 326, 537
DpnI GATC 3 cut(s) 418, 588, 627
DpnII GATC 3 cut(s) 416, 586, 625
DriI GACNNNNNGTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 486, 627
EcoICRI GAGCTC 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 563
FaeI CATG 2 cut(s) 186, 509
FaiI YATR 5 cut(s) 184, 374, 487, 507, 528
FaqI GGGAC 1 cut(s) 162
FatI CATG 2 cut(s) 182, 505
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 104, 107, 225
FokI GGATG 1 cut(s) 318
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 3 cut(s) 104, 107, 225
FspBI CTAG 3 cut(s) 251, 366, 564
GluI GCNGC 3 cut(s) 104, 107, 225
GsuI CTGGAG 2 cut(s) 157, 620
HaeIII GGCC 2 cut(s) 386, 638
HapII CCGG 1 cut(s) 383
Hin1II CATG 2 cut(s) 186, 509
HinfI GANTC 2 cut(s) 4, 541
HpaII CCGG 1 cut(s) 383
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 5 cut(s) 247, 327, 416, 442, 655
Hpy188III TCNNGA 5 cut(s) 137, 203, 290, 536, 629
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 197, 300
HpyCH4III ACNGT 4 cut(s) 241, 475, 484, 548
HpyCH4IV ACGT 2 cut(s) 84, 127
HpyCH4V TGCA 6 cut(s) 31, 40, 146, 227, 269, 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 366
HpyF3I CTNAG 3 cut(s) 212, 326, 537
HpySE526I ACGT 2 cut(s) 84, 127
Hsp92II CATG 2 cut(s) 186, 509
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Kzo9I GATC 3 cut(s) 416, 586, 625
LmnI GCTCC 1 cut(s) 213
Lsp1109I GCAGC 3 cut(s) 93, 115, 211
LweI GCATC 1 cut(s) 340
MaeI CTAG 3 cut(s) 251, 366, 564
MaeII ACGT 2 cut(s) 84, 127
MaeIII GTNAC 3 cut(s) 235, 488, 548
MalI GATC 3 cut(s) 418, 588, 627
MboI GATC 3 cut(s) 416, 586, 625
MboII GAAGA 2 cut(s) 125, 479
MfeI CAATTG 1 cut(s) 147
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 33, 210
MluCI AATT 4 cut(s) 56, 147, 167, 498
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 7 cut(s) 226, 321, 351, 366, 397, 534, 649
MroNI GCCGGC 1 cut(s) 382
MseI TTAA 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 106
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 1 cut(s) 383
MunI CAATTG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 37, 366
NaeI GCCGGC 1 cut(s) 384
NdeII GATC 3 cut(s) 416, 586, 625
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 2 cut(s) 186, 509
NlaIV GGNNCC 1 cut(s) 80
NmeAIII GCCGAG 1 cut(s) 428
NmuCI GTSAC 3 cut(s) 235, 488, 548
NspI RCATGY 1 cut(s) 186
NspV TTCGAA 1 cut(s) 54
PaeI GCATGC 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 541
PkrI GCNGC 3 cut(s) 105, 108, 226
Ple19I CGATCG 1 cut(s) 628
Ppu21I YACGTR 2 cut(s) 85, 128
Psp124BI GAGCTC 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 1 cut(s) 636
PstI CTGCAG 1 cut(s) 229
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 416
PvuI CGATCG 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 3 cut(s) 24, 87, 520
RsaNI GTAC 3 cut(s) 23, 86, 519
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 3 cut(s) 104, 107, 225
Sau3AI GATC 3 cut(s) 416, 586, 625
Sau96I GGNCC 1 cut(s) 636
ScaI AGTACT 1 cut(s) 24
SduI GDGCHC 2 cut(s) 33, 210
SetI ASST 8 cut(s) 20, 87, 108, 126, 130, 210, 237, 427
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 225
SfuI TTCGAA 1 cut(s) 54
SmlI CTYRAG 3 cut(s) 201, 420, 451
SmoI CTYRAG 3 cut(s) 201, 420, 451
SphI GCATGC 1 cut(s) 186
Sse9I AATT 4 cut(s) 56, 147, 167, 498
SsiI CCGC 1 cut(s) 180
SspMI CTAG 3 cut(s) 251, 366, 564
SstI GAGCTC 1 cut(s) 210
StyI CCWWGG 1 cut(s) 563
TaaI ACNGT 4 cut(s) 241, 475, 484, 548
TaiI ACGT 2 cut(s) 87, 130
TaqI TCGA 3 cut(s) 54, 624, 628
TaqII GACCGA 1 cut(s) 333
TasI AATT 4 cut(s) 56, 147, 167, 498
TatI WGTACW 2 cut(s) 22, 518
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 4 cut(s) 234, 553, 577, 603
TseFI GTSAC 3 cut(s) 235, 488, 548
TseI GCWGC 3 cut(s) 103, 106, 224
Tsp45I GTSAC 3 cut(s) 235, 488, 548
TspDTI ATGAA 2 cut(s) 315, 389
TspRI CASTG 4 cut(s) 234, 553, 577, 603
Vha464I CTTAAG 1 cut(s) 451
VneI GTGCAC 1 cut(s) 29
XapI RAATTY 2 cut(s) 167, 498
XceI RCATGY 1 cut(s) 186
XmaJI CCTAGG 1 cut(s) 563
XspI CTAG 3 cut(s) 251, 366, 564
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.