Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

28964639 .. 28967946

Gene Structure 3308 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g40720.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGCAGTGACAGACTTCTTCGCCGGAGAGATCATCACGGAGCTCCTAAAGAACCTGCTAACCATATCCAAAAAATCATGGACGTTCAAAGACAGCGCCAACGGCCTCATAGAATCCATACAACACCTCCTCCCCATAATCCAAGAAATCAAATACTCCGGCGTCGAGTTGCCGGCCATCCGCCAGTCTCAGCTCGACCGTCTCTCGGAGACTCTCCGGCAGGGCCATGAGCTGGCACACAAGGTCCTCCTCTCCTCTCGCTACAACATTTACAAGAACCTCCAATACGTCCGGAAAATGGAGAAGATCGAAAAGGCAGTCGCCAAGTTCATCCAGGGCCCATTGCAGGCCCATATCTTGGCCGACGTTCATCACATAAGGTTCGAGACCACCGAGCGTTTCGACCGTCTTGAAGGCTCCAGCCACCGCCTCGAGCAGAGCCTCGGCGCCATGAAGATTGGAGCTTGCGGTGGCGGCGGCGTGTCGTGGATGGAAGAGGCTGTGAGGCGCGTGGAGGAGGAGGGCCGGATGCAGTGGGAGAGCAGCAGTGAGTTGGTTTTGGGTTTGGGTTTTGGGTTGAAGAAAGTCAAGGAAATGGTTTTGGGTAGAGACGACTTGATCGTTGGGATCAGTGGGATTGGTGGCTCTGGAAAAACCACTCTTGTTAAGGAGTTGGTTAAGGATGATCATGTCCGATCTTATTTTGGTGAGAGGATCCTGTTCCTGACGGTGTCACAATCTCCGGATGTAGATGATCTGAAGGCCAAGATTTTGGGGTTCATTATGGGGGGTCAAGTTCTGAGTCCCACTTCTGTGCTTCCTCAAATAAACTTTCAATTTGAATGGAAGAACCCAACAAAGACCCTGGTGGTTTTGGATGATGTTTGGTCACTCTCAACGCTTCAGCCGCTGCTCTTCAACATTCCTGGTTGTAGAACCCTAGTGGTTTCACGTATCAAATTCCCAACTGCTGTTAAAGCCACCTATGACGTGGAGTTGCTGGGTGAAAATGAAGCAGTGTCTCTGTTTTGCCACTCTGCTTTTGGCCAAAAGTCGATTCCTCCTGGTGCTGATAGGAAATTGGTTAAGCAGATTGTTGATGAGTGTAAGAGGCTGCCTTTGGCTCTCAAAGTGATTGGGTCTTCATTACGAGACCAGCCTGAAATGATTTGGGCAAATGCGAGCAAAAGGTTGTCACGAGGGGAAAGTATTGGCGAGTCGCATGAGACCGACCTACTTGATCGAATGGCAATCAGTGTGAATTGCTTAAGCCCCAAAGTTAGGGAATGTTTCCTGGATTTGGGTGCCTTTCCAGAAGATAAGAAGATCCCTCTGGATGTTCTTATCAACATTTGGGTTGAAACCCATGATCACATTGATGAAGAACTGGCCTTTGCTATTCTTAACGAGCTAGCAGACAAGAATCTTGTCAGCTTAGTGAAAGATGCGAGAGAGGGAGATATATACAGCAGTTGCTATGACATCTCAGTTACACAACATGATGTCTTGCGAGACCTTGCTCTTCATTTGAGTAATAATGGCTCCATAAATCAACGAACTCGATTACTGATGCCAAGAAGAGAGATAGAACTTCCAAGAGAATGGGACAGGCATTCAGATGAGCCATTTCAAGCTCGAATTGTATCTATTCATACAGGTGAAATGACAGAGATGGACTGGTTCGAAATGGAATTCCCCAAGGCTGAAGTGCTCATCTTGAATTTCGCCTCCAACGAGTACATGTTACCTCCTTTTATTCGTCACATGCCAAAGCTGAAGGCTTTGATCATCATAAACTACAGCGCATCCAATGCAATTCTATATGAATTTTCTGTCTTGTCAAGTTTGAGCAACTTGAGAAGCCTTTGGCTTGAAAAAGTTTCAGTTCCTCCCTTGTCCCCTGCTACCTTCCCTTCCAAAAAAGTTCGCAAGATATCGTTAATCTTGTGTAAGATAAATAAGAGCCTCGATGAATCAGCATTTCCTCGGCTCTTAGAACTCACAATTGATCATTGTGATGATTTGTTCGAGCTGCCTTCAAGCATTTGTAGGATGCATTTGCTTACCAACCTAAGCATAACCAACTGTCACCATCTATGGCAGCTGCCAGCGGATTTGAGTAAGCTGAAGTCTCTACAAATCCTAAGGCTATATGCATGCCCATATCTGCAAAAACTTCCTCCTGACATATGTGAGCTCCTCTGCTTGAAGTACCTAAACATTTCACAATGCTGTAGTTTGAGGGGTCTTCCCTTAAACATTGGCAAGTTGGCAAGTTTGGAAAAGATTGACATGAGGGAATGCTCACAACTCAGGAATCTTCCAAGCTCTGCAGCAGCATTACCTTCTCTTGGTCGTGTAGTTTGTGATGAAGAGATTTCTTGGTTATGGAAGAAGGCATTGCCAGACGTTCATGTTCAGGTTGCAGAGAAACGTTTCGATCTCGACTGGCTTGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

821

Amino Acids

92.74

Weight (kDa)

6.17

Isoelectric Point (pI)

50.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	9 - 131	2.5e-14	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	195 - 351	3.3e-14	NB-ARC domain
LRR_14	PF23598	641 - 768	1e-10	Leucine-rich repeat region
LRR_14	PF23598	729 - 792	5.9e-07	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	63
AccB1I	GGYRCC	2 cut(s)	446, 1304
AccB7I	CCANNNNNTGG	2 cut(s)	232, 358
AccII	CGCG	1 cut(s)	510
AccIII	TCCGGA	2 cut(s)	291, 742
AciI	CCGC	7 cut(s)	181, 427, 468, 474, 477, 908, 2111
AclI	AACGTT	1 cut(s)	2434
AclWI	GGATC	4 cut(s)	635, 709, 722, 1321
AcoI	YGGCCR	3 cut(s)	174, 360, 1045
AcsI	RAATTY	4 cut(s)	959, 1691, 1720, 1826
AcuI	CTGAAG	5 cut(s)	779, 887, 1725, 1796, 2147
AcyI	GRCGYC	2 cut(s)	162, 447
AfaI	GTAC	2 cut(s)	1739, 2213
AfiI	CCNNNNNNNGG	8 cut(s)	205, 232, 298, 346, 358, 1281, 1300, 2351
AflII	CTTAAG	1 cut(s)	1267
AflIII	ACRYGT	1 cut(s)	1740
AjiI	CACGTC	1 cut(s)	991
AjnI	CCWGG	5 cut(s)	333, 864, 925, 1063, 1293
AjuI	GAANNNNNNNTTGG	2 cut(s)	1041, 1073
AleI	CACNNNNGTG	1 cut(s)	812
Alw21I	GWGCWC	3 cut(s)	45, 1713, 2199
AlwI	GGATC	4 cut(s)	635, 709, 722, 1321
AlwNI	CAGNNNCTG	1 cut(s)	910
Ama87I	CYCGRG	1 cut(s)	431
Aor13HI	TCCGGA	2 cut(s)	291, 742
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	8 cut(s)	543, 910, 1114, 2032, 2101, 2104, 2333, 2336
ApoI	RAATTY	4 cut(s)	959, 1691, 1720, 1826
ArsI	GACNNNNNNTTYG	2 cut(s)	303, 335
Asp700I	GAANNNNTTC	1 cut(s)	2435
AspLEI	GCGC	4 cut(s)	98, 449, 510, 1805
AspS9I	GGNCC	6 cut(s)	223, 244, 337, 338, 349, 523
AsuHPI	GGTGA	4 cut(s)	719, 1016, 1670, 2081
AsuII	TTCGAA	1 cut(s)	1683
AsuNHI	GCTAGC	1 cut(s)	1411
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	1 cut(s)	244
AxyI	CCTNAGG	1 cut(s)	2144
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	1 cut(s)	1047
BamHI	GGATCC	1 cut(s)	714
BanI	GGYRCC	2 cut(s)	446, 1304
BanII	GRGCYC	3 cut(s)	45, 341, 2199
BauI	CACGAG	1 cut(s)	1197
BbsI	GAAGAC	2 cut(s)	1134, 2240
Bbv12I	GWGCWC	3 cut(s)	45, 1713, 2199
BbvI	GCAGC	8 cut(s)	555, 897, 1101, 2019, 2091, 2113, 2345, 2348
BccI	CCATC	4 cut(s)	185, 484, 1666, 2100
BceAI	ACGGC	1 cut(s)	118
BciT130I	CCWGG	5 cut(s)	335, 866, 927, 1065, 1295
BclI	TGATCA	4 cut(s)	685, 1369, 1785, 2008
BfaI	CTAG	2 cut(s)	941, 1412
BfmI	CTRYAG	3 cut(s)	1798, 2233, 2331
BfoI	RGCGCY	2 cut(s)	99, 450
BfrI	CTTAAG	1 cut(s)	1267
BfuAI	ACCTGC	1 cut(s)	63
Bme1390I	CCNGG	5 cut(s)	335, 866, 927, 1065, 1295
Bme18I	GGWCC	1 cut(s)	244
BmeT110I	CYCGRG	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	991
BmgT120I	GGNCC	6 cut(s)	223, 244, 337, 338, 349, 523
BmiI	GGNNCC	6 cut(s)	339, 418, 448, 716, 1306, 1543
BmrFI	CCNGG	5 cut(s)	335, 866, 927, 1065, 1295
BmsI	GCATC	5 cut(s)	519, 1435, 1560, 1814, 2043
BmtI	GCTAGC	1 cut(s)	1415
BpiI	GAAGAC	2 cut(s)	1134, 2240
BpmI	CTGGAG	1 cut(s)	403
Bpu10I	CCTNAGC	1 cut(s)	2072
Bpu14I	TTCGAA	1 cut(s)	1683
BpuEI	CTTGAG	1 cut(s)	1876
BsaAI	YACGTR	1 cut(s)	953
BsaHI	GRCGYC	2 cut(s)	162, 447
BsaI	GGTCTC	4 cut(s)	380, 1146, 1220, 1506
BsaJI	CCNNGG	5 cut(s)	334, 442, 864, 1698, 1985
BsaWI	WCCGGW	2 cut(s)	291, 742
BsaXI	ACNNNNNCTCC	4 cut(s)	111, 114, 141, 144
Bsc4I	CCNNNNNNNGG	8 cut(s)	205, 232, 298, 346, 358, 1281, 1300, 2351
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	4 cut(s)	184, 1392, 1682, 2453
Bse21I	CCTNAGG	1 cut(s)	2144
Bse3DI	GCAATG	2 cut(s)	341, 2399
BseAI	TCCGGA	2 cut(s)	291, 742
BseBI	CCWGG	5 cut(s)	335, 866, 927, 1065, 1295
BseDI	CCNNGG	5 cut(s)	334, 442, 864, 1698, 1985
BseLI	CCNNNNNNNGG	8 cut(s)	205, 232, 298, 346, 358, 1281, 1300, 2351
BseMI	GCAATG	2 cut(s)	341, 2399
BseMII	CTCAG	4 cut(s)	203, 791, 1500, 2326
BseNI	ACTGG	4 cut(s)	184, 1392, 1682, 2453
BseRI	GAGGAG	6 cut(s)	119, 239, 244, 530, 533, 2189
BseSI	GKGCMC	1 cut(s)	341
BseXI	GCAGC	8 cut(s)	555, 897, 1101, 2019, 2091, 2113, 2345, 2348
BseYI	CCCAGC	1 cut(s)	1000
Bsh1236I	CGCG	1 cut(s)	510
Bsh1285I	CGRYCG	2 cut(s)	199, 406
BshNI	GGYRCC	2 cut(s)	446, 1304
BsiEI	CGRYCG	2 cut(s)	199, 406
BsiHKAI	GWGCWC	3 cut(s)	45, 1713, 2199
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	7 cut(s)	24, 159, 173, 217, 292, 526, 743
BslFI	GGGAC	3 cut(s)	789, 1619, 1882
BslI	CCNNNNNNNGG	8 cut(s)	205, 232, 298, 346, 358, 1281, 1300, 2351
BsmBI	CGTCTC	2 cut(s)	206, 603
BsmFI	GGGAC	3 cut(s)	789, 1619, 1882
BsmI	GAATGC	2 cut(s)	1612, 2306
Bso31I	GGTCTC	4 cut(s)	380, 1146, 1220, 1506
BsoBI	CYCGRG	1 cut(s)	431
Bsp119I	TTCGAA	1 cut(s)	1683
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	4 cut(s)	45, 341, 1713, 2199
Bsp13I	TCCGGA	2 cut(s)	291, 742
BspACI	CCGC	7 cut(s)	181, 427, 468, 474, 477, 908, 2111
BspCNI	CTCAG	4 cut(s)	202, 792, 1499, 2325
BspEI	TCCGGA	2 cut(s)	291, 742
BspFNI	CGCG	1 cut(s)	510
BspLI	GGNNCC	6 cut(s)	339, 418, 448, 716, 1306, 1543
BspMAI	CTGCAG	1 cut(s)	2335
BspMI	ACCTGC	1 cut(s)	63
BspOI	GCTAGC	1 cut(s)	1415
BspPI	GGATC	4 cut(s)	635, 709, 722, 1321
BspQI	GCTCTTC	2 cut(s)	920, 1527
BspT104I	TTCGAA	1 cut(s)	1683
BspT107I	GGYRCC	2 cut(s)	446, 1304
BspTI	CTTAAG	1 cut(s)	1267
BspTNI	GGTCTC	4 cut(s)	380, 1146, 1220, 1506
BsrDI	GCAATG	2 cut(s)	341, 2399
BsrFI	RCCGGY	1 cut(s)	172
BsrI	ACTGG	4 cut(s)	184, 1392, 1682, 2453
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	5 cut(s)	334, 442, 864, 1698, 1985
BssNI	GRCGYC	2 cut(s)	162, 447
BssSI	CACGAG	1 cut(s)	1197
BssT1I	CCWWGG	1 cut(s)	1698
Bst2BI	CACGAG	1 cut(s)	1197
Bst2UI	CCWGG	5 cut(s)	335, 866, 927, 1065, 1295
Bst4CI	ACNGT	4 cut(s)	200, 407, 730, 2087
Bst6I	CTCTTC	5 cut(s)	489, 920, 1527, 1573, 2367
BstACI	GRCGYC	2 cut(s)	162, 447
BstAFI	CTTAAG	1 cut(s)	1267
BstAPI	GCANNNNNTGC	1 cut(s)	1811
BstBAI	YACGTR	1 cut(s)	953
BstBI	TTCGAA	1 cut(s)	1683
BstC8I	GCNNGC	8 cut(s)	174, 234, 348, 466, 1183, 1413, 2109, 2158
BstDEI	CTNAG	8 cut(s)	189, 800, 1435, 1486, 1993, 2072, 2144, 2312
BstFNI	CGCG	1 cut(s)	510
BstH2I	RGCGCY	2 cut(s)	99, 450
BstHHI	GCGC	4 cut(s)	98, 449, 510, 1805
BstMCI	CGRYCG	2 cut(s)	199, 406
BstMWI	GCNNNNNNNGC	5 cut(s)	102, 474, 907, 977, 1811
BstNI	CCWGG	5 cut(s)	335, 866, 927, 1065, 1295
BstNSI	RCATGY	3 cut(s)	1744, 1768, 2160
BstSCI	CCNGG	5 cut(s)	333, 864, 925, 1063, 1293
BstSFI	CTRYAG	3 cut(s)	1798, 2233, 2331
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	1 cut(s)	510
BstV1I	GCAGC	8 cut(s)	555, 897, 1101, 2019, 2091, 2113, 2345, 2348
BstV2I	GAAGAC	2 cut(s)	1134, 2240
BstX2I	RGATCY	2 cut(s)	714, 1326
BstXI	CCANNNNNNTGG	2 cut(s)	772, 1602
BstYI	RGATCY	2 cut(s)	714, 1326
Bsu36I	CCTNAGG	1 cut(s)	2144
BtrI	CACGTC	1 cut(s)	991
BtsI	GCAGTG	4 cut(s)	12, 539, 553, 1023
BtsIMutI	CAGTG	6 cut(s)	12, 539, 553, 637, 1023, 1261
BveI	ACCTGC	1 cut(s)	63
Cac8I	GCNNGC	8 cut(s)	174, 234, 348, 466, 1183, 1413, 2109, 2158
CaiI	CAGNNNCTG	1 cut(s)	910
CfoI	GCGC	4 cut(s)	98, 449, 510, 1805
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	6 cut(s)	223, 244, 337, 338, 349, 523
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	2 cut(s)	1738, 2212
CviQI	GTAC	2 cut(s)	1738, 2212
DdeI	CTNAG	8 cut(s)	189, 800, 1435, 1486, 1993, 2072, 2144, 2312
DinI	GGCGCC	1 cut(s)	448
EaeI	YGGCCR	3 cut(s)	174, 360, 1045
Eam1104I	CTCTTC	5 cut(s)	489, 920, 1527, 1573, 2367
EarI	CTCTTC	5 cut(s)	489, 920, 1527, 1573, 2367
EciI	GGCGGA	1 cut(s)	170
Ecl136II	GAGCTC	2 cut(s)	43, 2197
Eco130I	CCWWGG	1 cut(s)	1698
Eco24I	GRGCYC	3 cut(s)	45, 341, 2199
Eco31I	GGTCTC	4 cut(s)	380, 1146, 1220, 1506
Eco32I	GATATC	1 cut(s)	1935
Eco47I	GGWCC	1 cut(s)	244
Eco53kI	GAGCTC	2 cut(s)	43, 2197
Eco57I	CTGAAG	5 cut(s)	779, 887, 1725, 1796, 2147
Eco81I	CCTNAGG	1 cut(s)	2144
Eco88I	CYCGRG	1 cut(s)	431
EcoICRI	GAGCTC	2 cut(s)	43, 2197
EcoO109I	RGGNCCY	2 cut(s)	244, 337
EcoRI	GAATTC	1 cut(s)	1691
EcoRII	CCWGG	5 cut(s)	333, 864, 925, 1063, 1293
EcoRV	GATATC	1 cut(s)	1935
EcoT14I	CCWWGG	1 cut(s)	1698
EcoT22I	ATGCAT	2 cut(s)	2058, 2158
EcoT38I	GRGCYC	3 cut(s)	45, 341, 2199
EgeI	GGCGCC	1 cut(s)	448
EheI	GGCGCC	1 cut(s)	448
ErhI	CCWWGG	1 cut(s)	1698
Esp3I	CGTCTC	2 cut(s)	206, 603
FaqI	GGGAC	3 cut(s)	789, 1619, 1882
FauNDI	CATATG	1 cut(s)	2189
FbaI	TGATCA	4 cut(s)	685, 1369, 1785, 2008
FriOI	GRGCYC	3 cut(s)	45, 341, 2199
FspBI	CTAG	2 cut(s)	941, 1412
GlaI	GCGC	4 cut(s)	97, 448, 509, 1804
GsaI	CCCAGC	1 cut(s)	1004
GsuI	CTGGAG	1 cut(s)	403
HaeII	RGCGCY	2 cut(s)	99, 450
HapII	CCGG	7 cut(s)	24, 159, 173, 217, 292, 526, 743
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	4 cut(s)	98, 449, 510, 1805
Hin1I	GRCGYC	2 cut(s)	162, 447
Hin6I	GCGC	4 cut(s)	96, 447, 508, 1803
HinP1I	GCGC	4 cut(s)	96, 447, 508, 1803
HinfI	GANTC	8 cut(s)	113, 211, 802, 1057, 1217, 1423, 1973, 2317
HpaII	CCGG	7 cut(s)	24, 159, 173, 217, 292, 526, 743
HphI	GGTGA	4 cut(s)	719, 1016, 1670, 2081
Hpy188I	TCNGA	5 cut(s)	208, 695, 759, 801, 1618
Hpy99I	CGWCG	2 cut(s)	167, 368
HpyAV	CCTTC	8 cut(s)	407, 754, 1771, 1918, 1923, 2046, 2355, 2389
HpyCH4III	ACNGT	4 cut(s)	200, 407, 730, 2087
HpyCH4IV	ACGT	7 cut(s)	83, 288, 366, 952, 990, 2409, 2434
HpyCH4V	TGCA	8 cut(s)	346, 532, 1814, 2056, 2156, 2170, 2333, 2426
HpyF10VI	GCNNNNNNNGC	5 cut(s)	102, 474, 907, 977, 1811
HpyF3I	CTNAG	8 cut(s)	189, 800, 1435, 1486, 1993, 2072, 2144, 2312
HpySE526I	ACGT	7 cut(s)	83, 288, 366, 952, 990, 2409, 2434
Hsp92I	GRCGYC	2 cut(s)	162, 447
HspAI	GCGC	4 cut(s)	96, 447, 508, 1803
KasI	GGCGCC	1 cut(s)	446
Kpn2I	TCCGGA	2 cut(s)	291, 742
KroI	GCCGGC	1 cut(s)	172
KroNI	GCCGGC	1 cut(s)	174
Ksp22I	TGATCA	4 cut(s)	685, 1369, 1785, 2008
LguI	GCTCTTC	2 cut(s)	920, 1527
LmnI	GCTCC	6 cut(s)	40, 48, 422, 461, 1547, 2202
Lsp1109I	GCAGC	8 cut(s)	555, 897, 1101, 2019, 2091, 2113, 2345, 2348
LweI	GCATC	5 cut(s)	519, 1435, 1560, 1814, 2043
MaeI	CTAG	2 cut(s)	941, 1412
MaeII	ACGT	7 cut(s)	83, 288, 366, 952, 990, 2409, 2434
MaeIII	GTNAC	8 cut(s)	7, 732, 888, 1194, 1489, 1743, 1760, 2087
MfeI	CAATTG	1 cut(s)	2004
MflI	RGATCY	2 cut(s)	714, 1326
MhlI	GDGCHC	4 cut(s)	45, 341, 1713, 2199
MlsI	TGGCCA	1 cut(s)	1047
MluNI	TGGCCA	1 cut(s)	1047
Mly113I	GGCGCC	1 cut(s)	447
MlyI	GAGTC	3 cut(s)	205, 811, 1226
MmeI	TCCRAC	1 cut(s)	1755
Mox20I	TGGCCA	1 cut(s)	1047
Mph1103I	ATGCAT	2 cut(s)	2058, 2158
MroI	TCCGGA	2 cut(s)	291, 742
MroNI	GCCGGC	1 cut(s)	172
MroXI	GAANNNNTTC	1 cut(s)	2435
MscI	TGGCCA	1 cut(s)	1047
MseI	TTAA	8 cut(s)	666, 678, 975, 1086, 1268, 1404, 1940, 2255
MslI	CAYNNNNRTG	5 cut(s)	812, 1377, 1617, 1656, 2016
Msp20I	TGGCCA	1 cut(s)	1047
MspA1I	CMGCKG	3 cut(s)	910, 2104, 2111
MspCI	CTTAAG	1 cut(s)	1267
MspI	CCGG	7 cut(s)	24, 159, 173, 217, 292, 526, 743
MspR9I	CCNGG	5 cut(s)	335, 866, 927, 1065, 1295
MunI	CAATTG	1 cut(s)	2004
Mva1269I	GAATGC	2 cut(s)	1612, 2306
MvaI	CCWGG	5 cut(s)	335, 866, 927, 1065, 1295
MvnI	CGCG	1 cut(s)	510
MwoI	GCNNNNNNNGC	5 cut(s)	102, 474, 907, 977, 1811
NaeI	GCCGGC	1 cut(s)	174
NarI	GGCGCC	1 cut(s)	447
NdeI	CATATG	1 cut(s)	2189
NgoMIV	GCCGGC	1 cut(s)	172
NheI	GCTAGC	1 cut(s)	1411
NlaIV	GGNNCC	6 cut(s)	339, 418, 448, 716, 1306, 1543
NmeAIII	GCCGAG	2 cut(s)	423, 1966
NmuCI	GTSAC	6 cut(s)	7, 732, 888, 1194, 1760, 2087
NsiI	ATGCAT	2 cut(s)	2058, 2158
NspI	RCATGY	3 cut(s)	1744, 1768, 2160
NspV	TTCGAA	1 cut(s)	1683
OliI	CACNNNNGTG	1 cut(s)	812
PaeI	GCATGC	1 cut(s)	2160
PaeR7I	CTCGAG	1 cut(s)	431
PciI	ACATGT	1 cut(s)	1740
PciSI	GCTCTTC	2 cut(s)	920, 1527
PcsI	WCGNNNNNNNCGW	3 cut(s)	390, 618, 1731
PctI	GAATGC	2 cut(s)	1612, 2306
PdiI	GCCGGC	1 cut(s)	174
PdmI	GAANNNNTTC	1 cut(s)	2435
PfeI	GAWTC	5 cut(s)	113, 1057, 1423, 1973, 2317
PflFI	GACNNNGTC	1 cut(s)	730
PflMI	CCANNNNNTGG	2 cut(s)	232, 358
PfoI	TCCNGGA	1 cut(s)	1293
PleI	GAGTC	3 cut(s)	205, 810, 1225
PluTI	GGCGCC	1 cut(s)	450
PpsI	GAGTC	3 cut(s)	205, 810, 1225
Ppu21I	YACGTR	1 cut(s)	953
PpuMI	RGGWCCY	1 cut(s)	244
PscI	ACATGT	1 cut(s)	1740
Psp124BI	GAGCTC	2 cut(s)	45, 2199
Psp1406I	AACGTT	1 cut(s)	2434
Psp5II	RGGWCCY	1 cut(s)	244
Psp6I	CCWGG	5 cut(s)	333, 864, 925, 1063, 1293
PspFI	CCCAGC	1 cut(s)	1000
PspGI	CCWGG	5 cut(s)	333, 864, 925, 1063, 1293
PspN4I	GGNNCC	6 cut(s)	339, 418, 448, 716, 1306, 1543
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	6 cut(s)	223, 244, 337, 338, 349, 523
PspPPI	RGGWCCY	1 cut(s)	244
PspXI	VCTCGAGB	1 cut(s)	431
PsrI	GAACNNNNNNTAC	2 cut(s)	269, 301
PstI	CTGCAG	1 cut(s)	2335
PstNI	CAGNNNCTG	1 cut(s)	910
PsuI	RGATCY	2 cut(s)	714, 1326
PsyI	GACNNNGTC	1 cut(s)	730
PvuII	CAGCTG	1 cut(s)	2104
RsaI	GTAC	2 cut(s)	1739, 2213
RsaNI	GTAC	2 cut(s)	1738, 2212
RseI	CAYNNNNRTG	5 cut(s)	812, 1377, 1617, 1656, 2016
SacI	GAGCTC	2 cut(s)	45, 2199
SapI	GCTCTTC	2 cut(s)	920, 1527
SaqAI	TTAA	8 cut(s)	666, 678, 975, 1086, 1268, 1404, 1940, 2255
Sau96I	GGNCC	6 cut(s)	223, 244, 337, 338, 349, 523
SchI	GAGTC	3 cut(s)	205, 811, 1226
ScrFI	CCNGG	5 cut(s)	335, 866, 927, 1065, 1295
SduI	GDGCHC	4 cut(s)	45, 341, 1713, 2199
SfaNI	GCATC	5 cut(s)	519, 1435, 1560, 1814, 2043
SfcI	CTRYAG	3 cut(s)	1798, 2233, 2331
SfoI	GGCGCC	1 cut(s)	448
Sfr274I	CTCGAG	1 cut(s)	431
SfuI	TTCGAA	1 cut(s)	1683
SinI	GGWCC	1 cut(s)	244
SlaI	CTCGAG	1 cut(s)	431
SmiMI	CAYNNNNRTG	5 cut(s)	812, 1377, 1617, 1656, 2016
SmlI	CTYRAG	3 cut(s)	431, 1267, 1855
SmoI	CTYRAG	3 cut(s)	431, 1267, 1855
SphI	GCATGC	1 cut(s)	2160
SsiI	CCGC	7 cut(s)	181, 427, 468, 474, 477, 908, 2111
SspDI	GGCGCC	1 cut(s)	446
SspMI	CTAG	2 cut(s)	941, 1412
SstI	GAGCTC	2 cut(s)	45, 2199
StyD4I	CCNGG	5 cut(s)	333, 864, 925, 1063, 1293
StyI	CCWWGG	1 cut(s)	1698
TaaI	ACNGT	4 cut(s)	200, 407, 730, 2087
TaiI	ACGT	7 cut(s)	86, 291, 369, 955, 993, 2412, 2437
TaqII	GACCGA	1 cut(s)	1244
TatI	WGTACW	1 cut(s)	1737
TauI	GCSGC	3 cut(s)	477, 480, 910
TfiI	GAWTC	5 cut(s)	113, 1057, 1423, 1973, 2317
Tru1I	TTAA	8 cut(s)	666, 678, 975, 1086, 1268, 1404, 1940, 2255
Tru9I	TTAA	8 cut(s)	666, 678, 975, 1086, 1268, 1404, 1940, 2255
TscAI	CASTG	6 cut(s)	12, 539, 553, 637, 1023, 1261
TseFI	GTSAC	6 cut(s)	7, 732, 888, 1194, 1760, 2087
TseI	GCWGC	8 cut(s)	543, 910, 1114, 2032, 2101, 2104, 2333, 2336
Tsp45I	GTSAC	6 cut(s)	7, 732, 888, 1194, 1760, 2087
TspGWI	ACGGA	1 cut(s)	53
TspRI	CASTG	6 cut(s)	12, 539, 553, 637, 1023, 1261
Tth111I	GACNNNGTC	1 cut(s)	730
Van91I	CCANNNNNTGG	2 cut(s)	232, 358
Vha464I	CTTAAG	1 cut(s)	1267
VpaK11BI	GGWCC	1 cut(s)	244
XapI	RAATTY	4 cut(s)	959, 1691, 1720, 1826
XceI	RCATGY	3 cut(s)	1744, 1768, 2160
XcmI	CCANNNNNNNNNTGG	2 cut(s)	988, 1040
XhoI	CTCGAG	1 cut(s)	431
XmnI	GAANNNNTTC	1 cut(s)	2435
XspI	CTAG	2 cut(s)	941, 1412
Zsp2I	ATGCAT	2 cut(s)	2058, 2158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.