Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

3079789 .. 3083263

Gene Structure 3475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1041800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGGCAGTGACGGACTTCTTCGTCGGCGAGATCACGACGGAGCTCCTGAAAAATTTGATCGCCCTCTCCCGCAAGTCATGCAACAGCAGAGGAATCGCCGAGGAACTCAAAACCTCAGTCGAGCAGCTCCTCCCCATAATCCAAGAAATCAAGTACTCCGGCGTTGAGCTACCGGCCGAACGGCAGTTCCAGCTCGACCGCATGTCGGAAGTTCTCCAGAACGGCCTCGAACTGGTCCAAAAAATCCTCGCGACACCGCGCTGGAACTTTTACAGGCACGTCCGATTGGCGAAAAAGATGGAGGCTTTGGAGAAGACAGTGTCCAGATTTCTTCAGGGCCCATTACAGGTCCATATCTTGGCCGACGTCCACCACATTAGGGCGGGGATGGATCAGCGGTTCGACCGGCTTGATTGGTCGAACCGGACGCTGGAACAGCGGCTTGGGACCTTAAAGATTGGGACTTGCGGGGGCGGGGGTGTCGGTGGCGGGTGGATGGAGGAGGCTGTGAGGAGGGTGGAGGAGGAAGGGCAGATGAAGTGGGAGGTGAGCAGTTCGGTGGTGTTAGGATTGGGGGTGGGGATGAAGACGGTGAAGGGAATGATTTTTGGGAGGGAGGATTTGAGGGTCGTTGGGATTTGTGGGATTGGTGGCTCTGGAAAAACCACCCTCGCTAGAGAGCTTTGCAAGGATGATCAAGTCAGATCTTATTTCAGTGATAGAATTCTGTTCCTGACTGTATCACAGTCTCCAGATGTGAACCAGTTGAAGGCCAAGATTGTGCAGTTCATCATGGGGAACCAAATTCTCAGTCCCAATTCTGTGCTTCCCCAATGGAACTTGCAATATGAATGGATTACCGCAACGAAAACTCTGGTGGTTTTGGATGATGTGTGGTCACTGGCAGTGCTTGAGCCGCTGCTCTTTAAGATCCCAGGCTGCAAGTTTCTTGTGGTCTCACGTTTCAAATTCCCAACCGTCCTCAATGCCACTTATGATGTTGAGTTGTTGAGGGAAGAAGAAGCACTCTCTCTGTTTTGTCACTCTGCTTTTGGCCAAAAGTCCATTCCTCCTGCTGCTGATAAAAATTTGGTCAAGCAGATTGTTAACGAGTGTAAGCGGCTTCCTTTGGCTCTCAAAGTGATTGGAGCTTCACTGAAGGACCAGCCTGAATTGTGTTGGGCAAATGCAAGGAGCAAGTTGTCGAAAGGGGAAGCTTTTTGTGAGTCCCATGAGAACAATCTACTTAAACGTATGGCAACGAGTCTCGACTGCTTATCCAAAAATGTTAAATCATGTTTCTTGGATTTGGGTGCGTTTCCAGAAGACAAGAAGATCCCTCTTGATGTCCTTACCAATATGTGGGTTGAAATACATGGCATCGACGAAGAAGAAGCGTTTGCTATTCTTGTTGAGCTTTCAAACAAAAATCTCCTCACCCTGGTGAAAGATGCAAGAGTTGGAGACATATATAGCAGTTGCTATGACATATCTGTTACACAACATGATGTGCTGAGGGACCTTGCTCTTCATTTGAGCAATGATGGCAGCGTGAACGAACGCAGTCGATTGCTTATGCCAAGAAGAGAGACTGAACTTCCAAGAGAATGGGAGAGGGATTCAGATCAGCCATTTCACGCGAAGATTGTATCAGTTCATACCGGTGAAATGAAAGAGATGGACTGGTTCCAGATGGAGTTTCCCAAATGTGAAGTGCTGATTTTGAATTTTTCATCGGACAACGATTACCTTCTTCCTCCTTTCATTGAAAACATGCCAAAGTTGAGGGCATTGATAATCATGAATTACAGTACGTCAAATTGCATCCTAAATGGCTTTTCAGTCTTCTCAAACTTAACCAACTTGAGAAGCCTCTGGCTCGAAAAAGTATTTGTCCCTCGGTTATCCACCATCCCCCTGAAAAATCTGAGGAAAATCTATATAATCCTATGCAAGATCAGTAATAGCCTTGATCAATCCACCTTTCCTCGGCTCACAGAGCTCACAATCGATCATTGCGATGACTTATTCCAGCTGCCTTCGTGCATTTGCACAATGCCTTCGCTCAAGAACTTGAGCGTAACCAACTGCCACAGCCTATACCAGCTCCCAGCTGATTTGGGAAGGCTGCAATCTCTACAGATCCTAAGGTTATACGCGTGTCCGGCACTGCAGACGCTTCCAGCTAGCATTTGCGAGCTACTTCGCTTGGAGTACATTGACATTTCCCAATGTGTAAATTTGAGATGCCTACCCATGGACATTGGTAAGCTAGCAAGTTTGGTAAAGATTGACATGAGGGAATGTTCACAAATTAGCAATCTGCCGAAATCCGCTGCAGCGTTACAGTCCCTGCGCCGTGTAGTGTGCGACGAGGATATTTCTTGGATGTGGAGAGATGCAGAGAAGGCATTGCCGGAGCTTCATGTTCAGGTGGTGGAGAAACATTTCGATCTGGACTGGCTCGATGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

825

Amino Acids

93.45

Weight (kDa)

6.03

Isoelectric Point (pI)

48.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	8 - 133	7.4e-12	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	196 - 354	2.7e-18	NB-ARC domain
LRR_14	PF23598	643 - 792	2e-13	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	20
AatII	GACGTC	1 cut(s)	369
AccB7I	CCANNNNNTGG	2 cut(s)	358, 1362
AccII	CGCG	4 cut(s)	251, 259, 1640, 2159
AclWI	GGATC	4 cut(s)	399, 925, 1330, 2137
AcoI	YGGCCR	3 cut(s)	174, 360, 1054
AcsI	RAATTY	7 cut(s)	52, 723, 804, 968, 1087, 1726, 2239
AcuI	CTGAAG	2 cut(s)	317, 1178
AcyI	GRCGYC	1 cut(s)	366
AfaI	GTAC	3 cut(s)	155, 1813, 2216
AflIII	ACRYGT	1 cut(s)	2157
AgeI	ACCGGT	1 cut(s)	1661
AgsI	TTSAA	6 cut(s)	769, 967, 1370, 1422, 1726, 1769
AhdI	GACNNNNNGTC	1 cut(s)	202
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	2 cut(s)	934, 1440
AjuI	GAANNNNNNNTTGG	4 cut(s)	426, 458, 1050, 1082
AleI	CACNNNNGTG	1 cut(s)	1442
AloI	GAACNNNNNNTCC	4 cut(s)	171, 203, 383, 415
Alw21I	GWGCWC	2 cut(s)	45, 2004
Alw26I	GTCTC	5 cut(s)	753, 961, 1271, 1458, 1583
AlwI	GGATC	4 cut(s)	399, 925, 1330, 2137
AlwNI	CAGNNNCTG	1 cut(s)	2353
AoxI	GGCC	6 cut(s)	174, 223, 337, 360, 771, 1054
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	9 cut(s)	124, 919, 939, 1076, 1548, 2035, 2128, 2336, 2339
ApoI	RAATTY	7 cut(s)	52, 723, 804, 968, 1087, 1726, 2239
ArsI	GACNNNNNNTTYG	4 cut(s)	102, 134, 796, 828
AsiGI	ACCGGT	1 cut(s)	1661
Asp700I	GAANNNNTTC	1 cut(s)	2447
AspLEI	GCGC	2 cut(s)	261, 2358
AspS9I	GGNCC	7 cut(s)	235, 337, 338, 349, 447, 1162, 1519
AsuHPI	GGTGA	5 cut(s)	559, 604, 1429, 1456, 1676
AsuNHI	GCTAGC	2 cut(s)	2186, 2272
AvaII	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
AxyI	CCTNAGG	1 cut(s)	2147
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	1 cut(s)	1056
BanII	GRGCYC	3 cut(s)	45, 341, 2004
BbsI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
Bbv12I	GWGCWC	2 cut(s)	45, 2004
BbvCI	CCTCAGC	1 cut(s)	1514
BbvI	GCAGC	9 cut(s)	136, 906, 926, 1063, 1560, 2022, 2115, 2323, 2351
BccI	CCATC	7 cut(s)	292, 382, 490, 1538, 1672, 1687, 1919
BceAI	ACGGC	3 cut(s)	197, 238, 2343
BcgI	CGANNNNNNTGC	2 cut(s)	76, 110
BciT130I	CCWGG	2 cut(s)	936, 1442
BclI	TGATCA	2 cut(s)	694, 1972
BcoDI	GTCTC	5 cut(s)	753, 961, 1271, 1458, 1583
BfaI	CTAG	3 cut(s)	675, 2187, 2273
BfmI	CTRYAG	3 cut(s)	2138, 2171, 2337
BglII	AGATCT	1 cut(s)	704
BmcAI	AGTACT	1 cut(s)	155
Bme1390I	CCNGG	2 cut(s)	936, 1442
Bme18I	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
BmeRI	GACNNNNNGTC	1 cut(s)	202
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	7 cut(s)	235, 337, 338, 349, 447, 1162, 1519
BmiI	GGNNCC	5 cut(s)	339, 448, 800, 1520, 1688
BmrFI	CCNGG	2 cut(s)	936, 1442
BmsI	GCATC	5 cut(s)	1389, 1441, 1833, 2237, 2389
BmtI	GCTAGC	2 cut(s)	2190, 2276
BpiI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BpmI	CTGGAG	2 cut(s)	200, 735
Bpu10I	CCTNAGC	1 cut(s)	1514
BpuEI	CTTGAG	4 cut(s)	932, 1885, 2051, 2095
Bsa29I	ATCGAT	1 cut(s)	2010
BsaBI	GATNNNNATC	1 cut(s)	1623
BsaHI	GRCGYC	1 cut(s)	366
BsaI	GGTCTC	1 cut(s)	961
BsaJI	CCNNGG	6 cut(s)	99, 934, 1440, 1898, 1988, 2256
BsaWI	WCCGGW	2 cut(s)	423, 1661
Bse118I	RCCGGY	3 cut(s)	172, 405, 1661
Bse1I	ACTGG	5 cut(s)	237, 763, 906, 1688, 2465
Bse21I	CCTNAGG	1 cut(s)	2147
Bse3DI	GCAATG	3 cut(s)	1546, 2014, 2411
Bse8I	GATNNNNATC	1 cut(s)	1623
BseBI	CCWGG	2 cut(s)	936, 1442
BseCI	ATCGAT	1 cut(s)	2010
BseDI	CCNNGG	6 cut(s)	99, 934, 1440, 1898, 1988, 2256
BseGI	GGATG	8 cut(s)	393, 501, 588, 697, 892, 1824, 1911, 2394
BseJI	GATNNNNATC	1 cut(s)	1623
BseMI	GCAATG	3 cut(s)	1546, 2014, 2411
BseMII	CTCAG	4 cut(s)	129, 823, 1505, 1919
BseNI	ACTGG	5 cut(s)	237, 763, 906, 1688, 2465
BseRI	GAGGAG	5 cut(s)	119, 515, 526, 536, 1424
BseSI	GKGCMC	1 cut(s)	341
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	9 cut(s)	136, 906, 926, 1063, 1560, 2022, 2115, 2323, 2351
BseYI	CCCAGC	1 cut(s)	2110
BsgI	GTGCAG	1 cut(s)	803
Bsh1236I	CGCG	4 cut(s)	251, 259, 1640, 2159
Bsh1285I	CGRYCG	3 cut(s)	177, 199, 406
BshFI	GGCC	6 cut(s)	176, 225, 339, 362, 773, 1056
BshTI	ACCGGT	1 cut(s)	1661
BshVI	ATCGAT	1 cut(s)	2010
BsiEI	CGRYCG	3 cut(s)	177, 199, 406
BsiHKAI	GWGCWC	2 cut(s)	45, 2004
BsiSI	CCGG	7 cut(s)	159, 173, 406, 424, 1662, 2165, 2417
BslFI	GGGAC	7 cut(s)	460, 475, 798, 1213, 1532, 1880, 2335
BsmAI	GTCTC	5 cut(s)	753, 961, 1271, 1458, 1583
BsmFI	GGGAC	7 cut(s)	460, 475, 798, 1213, 1532, 1880, 2335
BsnI	GGCC	6 cut(s)	176, 225, 339, 362, 773, 1056
Bso31I	GGTCTC	1 cut(s)	961
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	3 cut(s)	45, 341, 2004
Bsp19I	CCATGG	1 cut(s)	2256
Bsp68I	TCGCGA	1 cut(s)	251
BspANI	GGCC	6 cut(s)	176, 225, 339, 362, 773, 1056
BspCNI	CTCAG	4 cut(s)	128, 822, 1506, 1920
BspDI	ATCGAT	1 cut(s)	2010
BspFNI	CGCG	4 cut(s)	251, 259, 1640, 2159
BspHI	TCATGA	1 cut(s)	1800
BspLI	GGNNCC	5 cut(s)	339, 448, 800, 1520, 1688
BspMAI	CTGCAG	2 cut(s)	2175, 2341
BspOI	GCTAGC	2 cut(s)	2190, 2276
BspPI	GGATC	4 cut(s)	399, 925, 1330, 2137
BspQI	GCTCTTC	1 cut(s)	1533
BspTNI	GGTCTC	1 cut(s)	961
BsrDI	GCAATG	3 cut(s)	1546, 2014, 2411
BsrFI	RCCGGY	3 cut(s)	172, 405, 1661
BsrI	ACTGG	5 cut(s)	237, 763, 906, 1688, 2465
BssAI	RCCGGY	3 cut(s)	172, 405, 1661
BssECI	CCNNGG	6 cut(s)	99, 934, 1440, 1898, 1988, 2256
BssNI	GRCGYC	1 cut(s)	366
BssT1I	CCWWGG	1 cut(s)	2256
Bst2UI	CCWGG	2 cut(s)	936, 1442
Bst4CI	ACNGT	7 cut(s)	319, 592, 739, 747, 979, 1811, 2349
Bst6I	CTCTTC	2 cut(s)	1533, 1579
BstACI	GRCGYC	1 cut(s)	366
BstAPI	GCANNNNNTGC	1 cut(s)	78
BstC8I	GCNNGC	3 cut(s)	2188, 2198, 2274
BstDEI	CTNAG	5 cut(s)	115, 809, 1514, 1928, 2147
BstDSI	CCRYGG	1 cut(s)	2256
BstF5I	GGATG	8 cut(s)	393, 501, 588, 697, 892, 1824, 1911, 2394
BstFNI	CGCG	4 cut(s)	251, 259, 1640, 2159
BstHHI	GCGC	2 cut(s)	261, 2358
BstMAI	GTCTC	5 cut(s)	753, 961, 1271, 1458, 1583
BstMCI	CGRYCG	3 cut(s)	177, 199, 406
BstMWI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 1999, 2165, 2408
BstNI	CCWGG	2 cut(s)	936, 1442
BstNSI	RCATGY	2 cut(s)	205, 1777
BstSCI	CCNGG	2 cut(s)	934, 1440
BstSFI	CTRYAG	3 cut(s)	2138, 2171, 2337
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	4 cut(s)	251, 259, 1640, 2159
BstV1I	GCAGC	9 cut(s)	136, 906, 926, 1063, 1560, 2022, 2115, 2323, 2351
BstV2I	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BstX2I	RGATCY	4 cut(s)	704, 930, 1335, 2142
BstXI	CCANNNNNNTGG	1 cut(s)	1608
BstYI	RGATCY	4 cut(s)	704, 930, 1335, 2142
BstZI	CGGCCG	1 cut(s)	174
Bsu15I	ATCGAT	1 cut(s)	2010
Bsu36I	CCTNAGG	1 cut(s)	2147
BsuRI	GGCC	6 cut(s)	176, 225, 339, 362, 773, 1056
BsuTUI	ATCGAT	1 cut(s)	2010
BtgI	CCRYGG	1 cut(s)	2256
BtgZI	GCGATG	1 cut(s)	2034
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	8 cut(s)	393, 501, 588, 697, 892, 1824, 1911, 2394
BtsI	GCAGTG	3 cut(s)	12, 912, 2168
BtsIMutI	CAGTG	7 cut(s)	12, 324, 721, 899, 912, 1154, 2168
BtuMI	TCGCGA	1 cut(s)	251
Cac8I	GCNNGC	3 cut(s)	2188, 2198, 2274
CaiI	CAGNNNCTG	1 cut(s)	2353
CciI	TCATGA	1 cut(s)	1800
CfoI	GCGC	2 cut(s)	261, 2358
Cfr10I	RCCGGY	3 cut(s)	172, 405, 1661
Cfr13I	GGNCC	7 cut(s)	235, 337, 338, 349, 447, 1162, 1519
ClaI	ATCGAT	1 cut(s)	2010
CseI	GACGC	2 cut(s)	436, 2185
Csp6I	GTAC	3 cut(s)	154, 1812, 2215
CspAI	ACCGGT	1 cut(s)	1661
CviQI	GTAC	3 cut(s)	154, 1812, 2215
DdeI	CTNAG	5 cut(s)	115, 809, 1514, 1928, 2147
DrdI	GACNNNNNNGTC	1 cut(s)	20
DriI	GACNNNNNGTC	1 cut(s)	202
DseDI	GACNNNNNNGTC	1 cut(s)	20
EaeI	YGGCCR	3 cut(s)	174, 360, 1054
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	2 cut(s)	1533, 1579
Eam1105I	GACNNNNNGTC	1 cut(s)	202
EarI	CTCTTC	2 cut(s)	1533, 1579
Ecl136II	GAGCTC	2 cut(s)	43, 2002
EclXI	CGGCCG	1 cut(s)	174
Eco130I	CCWWGG	1 cut(s)	2256
Eco24I	GRGCYC	3 cut(s)	45, 341, 2004
Eco31I	GGTCTC	1 cut(s)	961
Eco47I	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
Eco52I	CGGCCG	1 cut(s)	174
Eco53kI	GAGCTC	2 cut(s)	43, 2002
Eco57I	CTGAAG	2 cut(s)	317, 1178
Eco81I	CCTNAGG	1 cut(s)	2147
EcoICRI	GAGCTC	2 cut(s)	43, 2002
EcoO109I	RGGNCCY	3 cut(s)	337, 447, 1519
EcoRI	GAATTC	1 cut(s)	723
EcoRII	CCWGG	2 cut(s)	934, 1440
EcoT14I	CCWWGG	1 cut(s)	2256
EcoT38I	GRGCYC	3 cut(s)	45, 341, 2004
ErhI	CCWWGG	1 cut(s)	2256
FaqI	GGGAC	7 cut(s)	460, 475, 798, 1213, 1532, 1880, 2335
FauI	CCCGC	5 cut(s)	77, 376, 461, 467, 482
FbaI	TGATCA	2 cut(s)	694, 1972
FokI	GGATG	8 cut(s)	400, 508, 595, 704, 899, 1811, 1898, 2401
FriOI	GRGCYC	3 cut(s)	45, 341, 2004
FspBI	CTAG	3 cut(s)	675, 2187, 2273
GlaI	GCGC	2 cut(s)	260, 2357
GsaI	CCCAGC	1 cut(s)	2114
GsuI	CTGGAG	2 cut(s)	200, 735
HaeIII	GGCC	6 cut(s)	176, 225, 339, 362, 773, 1056
HapII	CCGG	7 cut(s)	159, 173, 406, 424, 1662, 2165, 2417
HgaI	GACGC	2 cut(s)	436, 2185
HhaI	GCGC	2 cut(s)	261, 2358
Hin1I	GRCGYC	1 cut(s)	366
Hin6I	GCGC	2 cut(s)	259, 2356
HinP1I	GCGC	2 cut(s)	259, 2356
HincII	GTYRAC	1 cut(s)	1108
HindII	GTYRAC	1 cut(s)	1108
HindIII	AAGCTT	1 cut(s)	1215
HinfI	GANTC	4 cut(s)	93, 1226, 1264, 1619
HpaI	GTTAAC	1 cut(s)	1108
HpaII	CCGG	7 cut(s)	159, 173, 406, 424, 1662, 2165, 2417
HphI	GGTGA	5 cut(s)	559, 604, 1429, 1456, 1676
Hpy166II	GTNNAC	5 cut(s)	370, 760, 1108, 1555, 2309
Hpy188I	TCNGA	6 cut(s)	208, 284, 704, 1624, 1738, 1929
Hpy8I	GTNNAC	5 cut(s)	370, 760, 1108, 1555, 2309
Hpy99I	CGWCG	5 cut(s)	26, 40, 368, 1388, 2375
HpyAV	CCTTC	9 cut(s)	521, 589, 763, 1153, 1760, 2049, 2070, 2118, 2401
HpyCH4III	ACNGT	7 cut(s)	319, 592, 739, 747, 979, 1811, 2349
HpyCH4IV	ACGT	5 cut(s)	279, 366, 961, 1252, 1814
HpyF10VI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 1999, 2165, 2408
HpyF3I	CTNAG	5 cut(s)	115, 809, 1514, 1928, 2147
HpySE526I	ACGT	5 cut(s)	279, 366, 961, 1252, 1814
Hsp92I	GRCGYC	1 cut(s)	366
HspAI	GCGC	2 cut(s)	259, 2356
Ksp22I	TGATCA	2 cut(s)	694, 1972
KspAI	GTTAAC	1 cut(s)	1108
LguI	GCTCTTC	1 cut(s)	1533
LmnI	GCTCC	7 cut(s)	40, 48, 132, 1148, 1194, 2112, 2419
Lsp1109I	GCAGC	9 cut(s)	136, 906, 926, 1063, 1560, 2022, 2115, 2323, 2351
LweI	GCATC	5 cut(s)	1389, 1441, 1833, 2237, 2389
MaeI	CTAG	3 cut(s)	675, 2187, 2273
MaeII	ACGT	5 cut(s)	279, 366, 961, 1252, 1814
MaeIII	GTNAC	6 cut(s)	7, 897, 1040, 1495, 2080, 2343
MflI	RGATCY	4 cut(s)	704, 930, 1335, 2142
MhlI	GDGCHC	3 cut(s)	45, 341, 2004
MlsI	TGGCCA	1 cut(s)	1056
MluI	ACGCGT	1 cut(s)	2157
MluNI	TGGCCA	1 cut(s)	1056
MlyI	GAGTC	2 cut(s)	1235, 1273
MmeI	TCCRAC	2 cut(s)	186, 1441
Mox20I	TGGCCA	1 cut(s)	1056
MroXI	GAANNNNTTC	1 cut(s)	2447
MscI	TGGCCA	1 cut(s)	1056
MseI	TTAA	6 cut(s)	452, 927, 1107, 1248, 1290, 1856
MslI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2019
Msp20I	TGGCCA	1 cut(s)	1056
MspA1I	CMGCKG	6 cut(s)	397, 439, 919, 2035, 2114, 2336
MspI	CCGG	7 cut(s)	159, 173, 406, 424, 1662, 2165, 2417
MspR9I	CCNGG	2 cut(s)	936, 1442
MvaI	CCWGG	2 cut(s)	936, 1442
MvnI	CGCG	4 cut(s)	251, 259, 1640, 2159
MwoI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 1999, 2165, 2408
NcoI	CCATGG	1 cut(s)	2256
NheI	GCTAGC	2 cut(s)	2186, 2272
NlaIV	GGNNCC	5 cut(s)	339, 448, 800, 1520, 1688
NmeAIII	GCCGAG	2 cut(s)	124, 1969
NmuCI	GTSAC	3 cut(s)	7, 897, 1040
NruI	TCGCGA	1 cut(s)	251
NspI	RCATGY	2 cut(s)	205, 1777
OliI	CACNNNNGTG	1 cut(s)	1442
PagI	TCATGA	1 cut(s)	1800
PciSI	GCTCTTC	1 cut(s)	1533
PcsI	WCGNNNNNNNCGW	1 cut(s)	2016
PdmI	GAANNNNTTC	1 cut(s)	2447
PfeI	GAWTC	2 cut(s)	93, 1619
PflFI	GACNNNGTC	1 cut(s)	319
PflMI	CCANNNNNTGG	2 cut(s)	358, 1362
PinAI	ACCGGT	1 cut(s)	1661
PleI	GAGTC	2 cut(s)	1234, 1272
PpsI	GAGTC	2 cut(s)	1234, 1272
PpuMI	RGGWCCY	2 cut(s)	447, 1519
Psp124BI	GAGCTC	2 cut(s)	45, 2004
Psp5II	RGGWCCY	2 cut(s)	447, 1519
Psp6I	CCWGG	2 cut(s)	934, 1440
PspFI	CCCAGC	1 cut(s)	2110
PspGI	CCWGG	2 cut(s)	934, 1440
PspN4I	GGNNCC	5 cut(s)	339, 448, 800, 1520, 1688
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	7 cut(s)	235, 337, 338, 349, 447, 1162, 1519
PspPPI	RGGWCCY	2 cut(s)	447, 1519
PstI	CTGCAG	2 cut(s)	2175, 2341
PstNI	CAGNNNCTG	1 cut(s)	2353
PsuI	RGATCY	4 cut(s)	704, 930, 1335, 2142
PsyI	GACNNNGTC	1 cut(s)	319
PvuII	CAGCTG	2 cut(s)	2035, 2114
RruI	TCGCGA	1 cut(s)	251
RsaI	GTAC	3 cut(s)	155, 1813, 2216
RsaNI	GTAC	3 cut(s)	154, 1812, 2215
RseI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2019
SacI	GAGCTC	2 cut(s)	45, 2004
SapI	GCTCTTC	1 cut(s)	1533
SaqAI	TTAA	6 cut(s)	452, 927, 1107, 1248, 1290, 1856
Sau96I	GGNCC	7 cut(s)	235, 337, 338, 349, 447, 1162, 1519
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	2 cut(s)	1235, 1273
ScrFI	CCNGG	2 cut(s)	936, 1442
SduI	GDGCHC	3 cut(s)	45, 341, 2004
SfaNI	GCATC	5 cut(s)	1389, 1441, 1833, 2237, 2389
SfcI	CTRYAG	3 cut(s)	2138, 2171, 2337
SinI	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
SmiMI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2019
SmlI	CTYRAG	4 cut(s)	911, 1864, 2066, 2074
SmoI	CTYRAG	4 cut(s)	911, 1864, 2066, 2074
SspMI	CTAG	3 cut(s)	675, 2187, 2273
SstI	GAGCTC	2 cut(s)	45, 2004
StyD4I	CCNGG	2 cut(s)	934, 1440
StyI	CCWWGG	1 cut(s)	2256
TaaI	ACNGT	7 cut(s)	319, 592, 739, 747, 979, 1811, 2349
TaiI	ACGT	5 cut(s)	282, 369, 964, 1255, 1817
TatI	WGTACW	2 cut(s)	153, 2214
TauI	GCSGC	3 cut(s)	442, 919, 1123
TfiI	GAWTC	2 cut(s)	93, 1619
Tru1I	TTAA	6 cut(s)	452, 927, 1107, 1248, 1290, 1856
Tru9I	TTAA	6 cut(s)	452, 927, 1107, 1248, 1290, 1856
TscAI	CASTG	7 cut(s)	12, 324, 721, 906, 912, 1161, 2175
TseFI	GTSAC	3 cut(s)	7, 897, 1040
TseI	GCWGC	9 cut(s)	124, 919, 939, 1076, 1548, 2035, 2128, 2336, 2339
Tsp45I	GTSAC	3 cut(s)	7, 897, 1040
TspGWI	ACGGA	2 cut(s)	26, 53
TspRI	CASTG	7 cut(s)	12, 324, 721, 906, 912, 1161, 2175
Tth111I	GACNNNGTC	1 cut(s)	319
Van91I	CCANNNNNTGG	2 cut(s)	358, 1362
VpaK11BI	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
XapI	RAATTY	7 cut(s)	52, 723, 804, 968, 1087, 1726, 2239
XceI	RCATGY	2 cut(s)	205, 1777
XmnI	GAANNNNTTC	1 cut(s)	2447
XspI	CTAG	3 cut(s)	675, 2187, 2273
ZraI	GACGTC	1 cut(s)	367
ZrmI	AGTACT	1 cut(s)	155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.