Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

3115969 .. 3118979

Gene Structure 3011 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1042400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCAGTGACGGACTTCTTCGTCGGCGAGATCACAACGGAGCTCCTGAAAAATTTAATCGCCGCCATCTCCCTCAAGTCATGCAACAGCAGAGGAACCGCCGAGGAACTCAAAACCTTAGTCGAGCAGCTCCTCCCCATAATCCAAGAAATCAAGCACTCCGGCGTCGAGCTACCGGCCGAACGGCAGTTCCAGCTCGACCGCATGTCGGAAGTTCTCCAGAACGGCCTCGAACTGGTCCGAAAAGTCCTCACGACACCGCGCTGGAACTTTTACACGCACGTCCGATTGGCGAAAAAGATGGAGGCTTTGGAGACGACAGTGTCCAGATTTCTTCAGGGCCCATTACAGGTCCATATCTTGGCCGACGTCCACCACATTAGGGCGGAGGTGGATCAGCGGTTCGACCGGCTTGATTGGTCGAACCGGGTGCTGGAACAGCGGCTTGGGACCTTAAAGATTGGGACTTGCGGAGGCGGGGGTGTCGGTGGCGGGTGGATGGAGGAGGCTGTGAGGAGGGTGGAGGAGGAAGGGCAGATGAAGTGGGAGGTGAGCAGTTCGGTGGTGTTGGGATTGGGGGTGGGGATGAAGACGGTGAAGGGAATGATTTTTGGGAGGGAGGATTTGAGGGTCGTTGGGATTTGTGGGATTGGTGGCTCTGGAAAAACCACTCTCGCTAGAGAGCTTTGCAAGGATGATCAAGTTAGAACTTATTTCAATGAGAGAATTCTGTTCCTGACAGTGTCACAGTCTCCAGATGTGAACCAGTTGAAGGCCAAGATTGTGCAGTTCATCATGGGGAACCAAATTCTCAGTCCCAATTCTGTGCTTCCCCAATGGAACTTGCAATATGAATGGATAACCGTAACGAAATCTCTGGTGGTTTTGGATGATGTGTGGTCACTGGCAGTGCTTGAGCCGCTCCTCTTTAAGATCCCAGGCTGCAAGTTTCTTGTGGTCTCACGTTTCAAATTCCCAAACGTTCTCGATGCCACTTATGATGTTGAGTTGTTGAGGGAAGAAGAAGCACTCTCTCTGTTTTTTCACTCTGCTTTTGGCCAAAACTCCATTCCTCCTGCGGCTGATAAAAATTTGGTCAAGCAGATTGTTAACGAGTGCAAGCAGCTTCCTTTGGCTCTCAAAGTGATTGGAGCTTCATTGAAGGACCAGCCTGAATTGTGTTGGGCAAATGCAAGGAGCAAGTTGTCTAAAGGGGAAGCTTTTTGTGAGTCCCATGAGAACAATCTACTTAAACGTATGGCAACGAGTCTCGACTGCTTATCCAAAAAAGTTAAATCATGTTTCTTGGATTTGGGTGCGTTTCCAGAAGACAAGAAGATCCCTCTTGATGTCCTTACCAATATGTGGGTTGAAATACATGGCATTGACGAAGAAGAAGCGTTTGCTATTCTTGTTGAGCTTTCAAACAAAAATCTCCTCACCCTGGTGAAAGATGCAAGAGATGGAGACATATATAGCAGTTGCTATGACATATCTGTTACACAACATGATGTGCTGAGGGACCTTGCTCTTCATTTGAGCAGTGATGGCAGTGTGAATGAACGTAGTCGATTGCTTATGCCAAGAAGAGAGACTGAACTTCCAAGAGAATGGGAGATGGATTCAGATCAGCCATTTCATGCGAAGATTGTATCAGTTCATACCGGTGAAATGAAAGAGATGGACTGGTTCCAGATGGAGTTTCCCAAATGTGAAGTGCTGATTTTGAATTTTTCATCCGACGACGAGTACCTTTTGCCTCCTTTTATTGAAAACATGCCAAAGTTGAGGGCATTGATAATCATGAATTACAGTACATCAAATGCCATCCTAAATGGCTTTTCAGTCTTCTCAAATTTATCCAACTTGAGAAGCCTCTGGCTCGAAAAAGTGTCTGTCCCTCAATTAGCTACCATCCCCCTGAAAAATCTGAGGAAAATCTATATAATCCTATGCAAGATCAGTAATAGCCTTGATCAATCCACCTTTCCTCGGCTGACAGAGCTCACAATCGATCATTGCGATGACTTATTCCAGCTGCCTTCGTGCATTTGCGCAATGCCTTCGCTCAAGAACTTGAGCGTAACCAACTGCCACAGCCTATACCAGCTCCCAGCTGATTTGGGAAAGCTGCAATCTCTACAGATCCTAAGGCTATATGCGTGTGCGGCACTGCAGACGCTTCCGGCCAGCATTTGCGAGTTACTTCGCTTGAAGTACGTTGACATTTGCCAGTGTGTAAATTTGAGATGCCTTCCCATGGACATTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

758

Amino Acids

85.42

Weight (kDa)

5.76

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	11 - 133	5e-09	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	197 - 354	5.1e-17	NB-ARC domain
LRR_14	PF23598	644 - 749	8e-09	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	20, 322
AatII	GACGTC	1 cut(s)	372
Acc16I	TGCGCA	1 cut(s)	2056
AccB7I	CCANNNNNTGG	2 cut(s)	361, 1365
AccBSI	CCGCTC	1 cut(s)	922
AccII	CGCG	1 cut(s)	262
AclI	AACGTT	1 cut(s)	981
AclWI	GGATC	4 cut(s)	402, 928, 1333, 2140
AcoI	YGGCCR	4 cut(s)	177, 363, 1057, 2187
AcsI	RAATTY	8 cut(s)	52, 726, 807, 971, 1090, 1729, 1855, 2242
AcuI	CTGAAG	1 cut(s)	320
AcyI	GRCGYC	2 cut(s)	165, 369
AfaI	GTAC	3 cut(s)	1751, 1816, 2219
AgeI	ACCGGT	1 cut(s)	1664
AgsI	TTSAA	9 cut(s)	718, 772, 970, 1162, 1373, 1425, 1729, 1772, 2215
AhdI	GACNNNNNGTC	1 cut(s)	205
AjiI	CACGTC	1 cut(s)	283
AjnI	CCWGG	2 cut(s)	937, 1443
AjuI	GAANNNNNNNTTGG	4 cut(s)	429, 461, 1053, 1085
AleI	CACNNNNGTG	1 cut(s)	1445
AloI	GAACNNNNNNTCC	4 cut(s)	174, 206, 386, 418
Alw21I	GWGCWC	2 cut(s)	45, 2007
Alw26I	GTCTC	6 cut(s)	308, 756, 964, 1274, 1461, 1586
AlwI	GGATC	4 cut(s)	402, 928, 1333, 2140
AoxI	GGCC	7 cut(s)	177, 226, 340, 363, 774, 1057, 2187
ApaI	GGGCCC	1 cut(s)	344
ApeKI	GCWGC	5 cut(s)	127, 942, 1123, 2038, 2131
ApoI	RAATTY	8 cut(s)	52, 726, 807, 971, 1090, 1729, 1855, 2242
ArsI	GACNNNNNNTTYG	4 cut(s)	105, 137, 799, 831
AsiGI	ACCGGT	1 cut(s)	1664
AspLEI	GCGC	2 cut(s)	264, 2057
AspS9I	GGNCC	7 cut(s)	238, 340, 341, 352, 450, 1165, 1522
AsuC2I	CCSGG	1 cut(s)	428
AsuHPI	GGTGA	5 cut(s)	562, 607, 1432, 1459, 1679
AvaII	GGWCC	5 cut(s)	238, 352, 450, 1165, 1522
AxyI	CCTNAGG	1 cut(s)	2150
BaeGI	GKGCMC	1 cut(s)	344
BalI	TGGCCA	1 cut(s)	1059
BanII	GRGCYC	3 cut(s)	45, 344, 2007
BbsI	GAAGAC	3 cut(s)	596, 1335, 1840
Bbv12I	GWGCWC	2 cut(s)	45, 2007
BbvCI	CCTCAGC	1 cut(s)	1517
BbvI	GCAGC	5 cut(s)	139, 929, 1135, 2025, 2118
BceAI	ACGGC	2 cut(s)	200, 241
BcgI	CGANNNNNNTGC	4 cut(s)	412, 446, 2046, 2080
BciT130I	CCWGG	2 cut(s)	939, 1445
BclI	TGATCA	2 cut(s)	697, 1975
BcnI	CCSGG	1 cut(s)	428
BcoDI	GTCTC	6 cut(s)	308, 756, 964, 1274, 1461, 1586
BfaI	CTAG	1 cut(s)	678
BfmI	CTRYAG	2 cut(s)	2141, 2174
Bme1390I	CCNGG	3 cut(s)	428, 939, 1445
Bme18I	GGWCC	5 cut(s)	238, 352, 450, 1165, 1522
BmeRI	GACNNNNNGTC	1 cut(s)	205
BmgBI	CACGTC	1 cut(s)	283
BmgT120I	GGNCC	7 cut(s)	238, 340, 341, 352, 450, 1165, 1522
BmiI	GGNNCC	6 cut(s)	97, 342, 451, 803, 1523, 1691
BmrFI	CCNGG	3 cut(s)	428, 939, 1445
BmsI	GCATC	3 cut(s)	979, 1444, 2240
BpiI	GAAGAC	3 cut(s)	596, 1335, 1840
BpmI	CTGGAG	2 cut(s)	203, 738
Bpu10I	CCTNAGC	1 cut(s)	1517
BpuEI	CTTGAG	5 cut(s)	59, 935, 1888, 2054, 2098
BpuMI	CCSGG	1 cut(s)	428
Bsa29I	ATCGAT	1 cut(s)	2013
BsaBI	GATNNNNATC	1 cut(s)	1626
BsaHI	GRCGYC	2 cut(s)	165, 369
BsaI	GGTCTC	1 cut(s)	964
BsaJI	CCNNGG	5 cut(s)	102, 937, 1443, 1991, 2259
BsaWI	WCCGGW	1 cut(s)	1664
Bse118I	RCCGGY	3 cut(s)	175, 408, 1664
Bse1I	ACTGG	5 cut(s)	240, 766, 909, 1691, 2233
Bse21I	CCTNAGG	1 cut(s)	2150
Bse3DI	GCAATG	2 cut(s)	2017, 2064
Bse8I	GATNNNNATC	1 cut(s)	1626
BseBI	CCWGG	2 cut(s)	939, 1445
BseCI	ATCGAT	1 cut(s)	2013
BseDI	CCNNGG	5 cut(s)	102, 937, 1443, 1991, 2259
BseGI	GGATG	7 cut(s)	504, 591, 700, 895, 1736, 1827, 1914
BseJI	GATNNNNATC	1 cut(s)	1626
BseMI	GCAATG	2 cut(s)	2017, 2064
BseMII	CTCAG	3 cut(s)	826, 1508, 1922
BseNI	ACTGG	5 cut(s)	240, 766, 909, 1691, 2233
BseRI	GAGGAG	6 cut(s)	122, 518, 529, 539, 914, 1427
BseSI	GKGCMC	1 cut(s)	344
BseX3I	CGGCCG	1 cut(s)	177
BseXI	GCAGC	5 cut(s)	139, 929, 1135, 2025, 2118
BseYI	CCCAGC	1 cut(s)	2113
BsgI	GTGCAG	1 cut(s)	806
Bsh1236I	CGCG	1 cut(s)	262
Bsh1285I	CGRYCG	3 cut(s)	180, 202, 409
BshFI	GGCC	7 cut(s)	179, 228, 342, 365, 776, 1059, 2189
BshTI	ACCGGT	1 cut(s)	1664
BshVI	ATCGAT	1 cut(s)	2013
BsiEI	CGRYCG	3 cut(s)	180, 202, 409
BsiHKAI	GWGCWC	2 cut(s)	45, 2007
BsiSI	CCGG	6 cut(s)	162, 176, 409, 427, 1665, 2186
BslFI	GGGAC	6 cut(s)	463, 478, 801, 1216, 1535, 1883
BsmAI	GTCTC	6 cut(s)	308, 756, 964, 1274, 1461, 1586
BsmBI	CGTCTC	1 cut(s)	308
BsmFI	GGGAC	6 cut(s)	463, 478, 801, 1216, 1535, 1883
BsnI	GGCC	7 cut(s)	179, 228, 342, 365, 776, 1059, 2189
Bso31I	GGTCTC	1 cut(s)	964
Bsp120I	GGGCCC	1 cut(s)	340
Bsp1286I	GDGCHC	3 cut(s)	45, 344, 2007
Bsp19I	CCATGG	1 cut(s)	2259
BspANI	GGCC	7 cut(s)	179, 228, 342, 365, 776, 1059, 2189
BspCNI	CTCAG	3 cut(s)	825, 1509, 1923
BspDI	ATCGAT	1 cut(s)	2013
BspFNI	CGCG	1 cut(s)	262
BspHI	TCATGA	1 cut(s)	1803
BspLI	GGNNCC	6 cut(s)	97, 342, 451, 803, 1523, 1691
BspMAI	CTGCAG	1 cut(s)	2178
BspPI	GGATC	4 cut(s)	402, 928, 1333, 2140
BspQI	GCTCTTC	1 cut(s)	1536
BspTNI	GGTCTC	1 cut(s)	964
BsrBI	CCGCTC	1 cut(s)	922
BsrDI	GCAATG	2 cut(s)	2017, 2064
BsrFI	RCCGGY	3 cut(s)	175, 408, 1664
BsrI	ACTGG	5 cut(s)	240, 766, 909, 1691, 2233
BssAI	RCCGGY	3 cut(s)	175, 408, 1664
BssECI	CCNNGG	5 cut(s)	102, 937, 1443, 1991, 2259
BssNI	GRCGYC	2 cut(s)	165, 369
BssT1I	CCWWGG	1 cut(s)	2259
Bst2UI	CCWGG	2 cut(s)	939, 1445
Bst4CI	ACNGT	6 cut(s)	322, 595, 742, 750, 865, 1814
Bst6I	CTCTTC	2 cut(s)	1536, 1582
BstACI	GRCGYC	2 cut(s)	165, 369
BstC8I	GCNNGC	2 cut(s)	1121, 2191
BstDEI	CTNAG	5 cut(s)	118, 812, 1517, 1931, 2150
BstDSI	CCRYGG	1 cut(s)	2259
BstF5I	GGATG	7 cut(s)	504, 591, 700, 895, 1736, 1827, 1914
BstFNI	CGCG	1 cut(s)	262
BstHHI	GCGC	2 cut(s)	264, 2057
BstMAI	GTCTC	6 cut(s)	308, 756, 964, 1274, 1461, 1586
BstMCI	CGRYCG	3 cut(s)	180, 202, 409
BstMWI	GCNNNNNNNGC	6 cut(s)	193, 439, 919, 1548, 2002, 2168
BstNI	CCWGG	2 cut(s)	939, 1445
BstNSI	RCATGY	2 cut(s)	208, 1780
BstSCI	CCNGG	3 cut(s)	426, 937, 1443
BstSFI	CTRYAG	2 cut(s)	2141, 2174
BstSLI	GKGCMC	1 cut(s)	344
BstUI	CGCG	1 cut(s)	262
BstV1I	GCAGC	5 cut(s)	139, 929, 1135, 2025, 2118
BstV2I	GAAGAC	3 cut(s)	596, 1335, 1840
BstX2I	RGATCY	3 cut(s)	933, 1338, 2145
BstXI	CCANNNNNNTGG	1 cut(s)	1611
BstYI	RGATCY	3 cut(s)	933, 1338, 2145
BstZI	CGGCCG	1 cut(s)	177
Bsu15I	ATCGAT	1 cut(s)	2013
Bsu36I	CCTNAGG	1 cut(s)	2150
BsuRI	GGCC	7 cut(s)	179, 228, 342, 365, 776, 1059, 2189
BsuTUI	ATCGAT	1 cut(s)	2013
BtgI	CCRYGG	1 cut(s)	2259
BtgZI	GCGATG	1 cut(s)	2037
BtrI	CACGTC	1 cut(s)	283
BtsCI	GGATG	7 cut(s)	504, 591, 700, 895, 1736, 1827, 1914
BtsI	GCAGTG	5 cut(s)	12, 915, 1549, 1558, 2171
BtsIMutI	CAGTG	9 cut(s)	12, 327, 747, 902, 915, 1549, 1558, 2171, 2240
Cac8I	GCNNGC	2 cut(s)	1121, 2191
CciI	TCATGA	1 cut(s)	1803
CfoI	GCGC	2 cut(s)	264, 2057
Cfr10I	RCCGGY	3 cut(s)	175, 408, 1664
Cfr13I	GGNCC	7 cut(s)	238, 340, 341, 352, 450, 1165, 1522
ClaI	ATCGAT	1 cut(s)	2013
CseI	GACGC	2 cut(s)	154, 2188
Csp6I	GTAC	3 cut(s)	1750, 1815, 2218
CspAI	ACCGGT	1 cut(s)	1664
CviQI	GTAC	3 cut(s)	1750, 1815, 2218
DdeI	CTNAG	5 cut(s)	118, 812, 1517, 1931, 2150
DrdI	GACNNNNNNGTC	2 cut(s)	20, 322
DriI	GACNNNNNGTC	1 cut(s)	205
DseDI	GACNNNNNNGTC	2 cut(s)	20, 322
EaeI	YGGCCR	4 cut(s)	177, 363, 1057, 2187
EagI	CGGCCG	1 cut(s)	177
Eam1104I	CTCTTC	2 cut(s)	1536, 1582
Eam1105I	GACNNNNNGTC	1 cut(s)	205
EarI	CTCTTC	2 cut(s)	1536, 1582
EciI	GGCGGA	1 cut(s)	401
Ecl136II	GAGCTC	2 cut(s)	43, 2005
EclXI	CGGCCG	1 cut(s)	177
Eco130I	CCWWGG	1 cut(s)	2259
Eco24I	GRGCYC	3 cut(s)	45, 344, 2007
Eco31I	GGTCTC	1 cut(s)	964
Eco47I	GGWCC	5 cut(s)	238, 352, 450, 1165, 1522
Eco52I	CGGCCG	1 cut(s)	177
Eco53kI	GAGCTC	2 cut(s)	43, 2005
Eco57I	CTGAAG	1 cut(s)	320
Eco81I	CCTNAGG	1 cut(s)	2150
EcoICRI	GAGCTC	2 cut(s)	43, 2005
EcoO109I	RGGNCCY	3 cut(s)	340, 450, 1522
EcoRI	GAATTC	1 cut(s)	726
EcoRII	CCWGG	2 cut(s)	937, 1443
EcoT14I	CCWWGG	1 cut(s)	2259
EcoT38I	GRGCYC	3 cut(s)	45, 344, 2007
ErhI	CCWWGG	1 cut(s)	2259
Esp3I	CGTCTC	1 cut(s)	308
FaqI	GGGAC	6 cut(s)	463, 478, 801, 1216, 1535, 1883
FauI	CCCGC	2 cut(s)	470, 485
FbaI	TGATCA	2 cut(s)	697, 1975
FokI	GGATG	7 cut(s)	511, 598, 707, 902, 1723, 1814, 1901
FriOI	GRGCYC	3 cut(s)	45, 344, 2007
FspBI	CTAG	1 cut(s)	678
FspI	TGCGCA	1 cut(s)	2056
GlaI	GCGC	2 cut(s)	263, 2056
GsaI	CCCAGC	1 cut(s)	2117
GsuI	CTGGAG	2 cut(s)	203, 738
HaeIII	GGCC	7 cut(s)	179, 228, 342, 365, 776, 1059, 2189
HapII	CCGG	6 cut(s)	162, 176, 409, 427, 1665, 2186
HgaI	GACGC	2 cut(s)	154, 2188
HhaI	GCGC	2 cut(s)	264, 2057
Hin1I	GRCGYC	2 cut(s)	165, 369
Hin6I	GCGC	2 cut(s)	262, 2055
HinP1I	GCGC	2 cut(s)	262, 2055
HincII	GTYRAC	2 cut(s)	1111, 2224
HindII	GTYRAC	2 cut(s)	1111, 2224
HindIII	AAGCTT	1 cut(s)	1218
HinfI	GANTC	3 cut(s)	1229, 1267, 1622
HpaI	GTTAAC	1 cut(s)	1111
HpaII	CCGG	6 cut(s)	162, 176, 409, 427, 1665, 2186
HphI	GGTGA	5 cut(s)	562, 607, 1432, 1459, 1679
Hpy166II	GTNNAC	4 cut(s)	373, 763, 1111, 2224
Hpy188I	TCNGA	6 cut(s)	211, 242, 287, 1627, 1741, 1932
Hpy8I	GTNNAC	4 cut(s)	373, 763, 1111, 2224
Hpy99I	CGWCG	5 cut(s)	26, 170, 371, 1745, 1748
HpyAV	CCTTC	7 cut(s)	524, 592, 766, 1156, 2052, 2073, 2264
HpyCH4III	ACNGT	6 cut(s)	322, 595, 742, 750, 865, 1814
HpyCH4IV	ACGT	7 cut(s)	282, 369, 964, 981, 1255, 1564, 2220
HpyF10VI	GCNNNNNNNGC	6 cut(s)	193, 439, 919, 1548, 2002, 2168
HpyF3I	CTNAG	5 cut(s)	118, 812, 1517, 1931, 2150
HpySE526I	ACGT	7 cut(s)	282, 369, 964, 981, 1255, 1564, 2220
Hsp92I	GRCGYC	2 cut(s)	165, 369
HspAI	GCGC	2 cut(s)	262, 2055
Ksp22I	TGATCA	2 cut(s)	697, 1975
KspAI	GTTAAC	1 cut(s)	1111
LguI	GCTCTTC	1 cut(s)	1536
LmnI	GCTCC	7 cut(s)	40, 48, 135, 927, 1151, 1197, 2115
Lsp1109I	GCAGC	5 cut(s)	139, 929, 1135, 2025, 2118
LweI	GCATC	3 cut(s)	979, 1444, 2240
MaeI	CTAG	1 cut(s)	678
MaeII	ACGT	7 cut(s)	282, 369, 964, 981, 1255, 1564, 2220
MaeIII	GTNAC	7 cut(s)	7, 744, 865, 900, 1498, 2083, 2202
MbiI	CCGCTC	1 cut(s)	922
MflI	RGATCY	3 cut(s)	933, 1338, 2145
MhlI	GDGCHC	3 cut(s)	45, 344, 2007
MlsI	TGGCCA	1 cut(s)	1059
MluNI	TGGCCA	1 cut(s)	1059
MlyI	GAGTC	2 cut(s)	1238, 1276
MmeI	TCCRAC	3 cut(s)	189, 1764, 1887
Mox20I	TGGCCA	1 cut(s)	1059
MscI	TGGCCA	1 cut(s)	1059
MseI	TTAA	7 cut(s)	56, 455, 930, 1110, 1251, 1293, 2272
MslI	CAYNNNNRTG	3 cut(s)	1445, 1665, 2022
Msp20I	TGGCCA	1 cut(s)	1059
MspA1I	CMGCKG	4 cut(s)	400, 442, 2038, 2117
MspI	CCGG	6 cut(s)	162, 176, 409, 427, 1665, 2186
MspR9I	CCNGG	3 cut(s)	428, 939, 1445
MvaI	CCWGG	2 cut(s)	939, 1445
MvnI	CGCG	1 cut(s)	262
MwoI	GCNNNNNNNGC	6 cut(s)	193, 439, 919, 1548, 2002, 2168
NciI	CCSGG	1 cut(s)	428
NcoI	CCATGG	1 cut(s)	2259
NlaIV	GGNNCC	6 cut(s)	97, 342, 451, 803, 1523, 1691
NmeAIII	GCCGAG	2 cut(s)	127, 1972
NmuCI	GTSAC	3 cut(s)	7, 744, 900
NsbI	TGCGCA	1 cut(s)	2056
NspI	RCATGY	2 cut(s)	208, 1780
OliI	CACNNNNGTG	1 cut(s)	1445
PagI	TCATGA	1 cut(s)	1803
PciSI	GCTCTTC	1 cut(s)	1536
PcsI	WCGNNNNNNNCGW	1 cut(s)	2019
PfeI	GAWTC	1 cut(s)	1622
PflFI	GACNNNGTC	2 cut(s)	322, 742
PflMI	CCANNNNNTGG	2 cut(s)	361, 1365
PinAI	ACCGGT	1 cut(s)	1664
PleI	GAGTC	2 cut(s)	1237, 1275
PpsI	GAGTC	2 cut(s)	1237, 1275
PpuMI	RGGWCCY	2 cut(s)	450, 1522
Psp124BI	GAGCTC	2 cut(s)	45, 2007
Psp1406I	AACGTT	1 cut(s)	981
Psp5II	RGGWCCY	2 cut(s)	450, 1522
Psp6I	CCWGG	2 cut(s)	937, 1443
PspFI	CCCAGC	1 cut(s)	2113
PspGI	CCWGG	2 cut(s)	937, 1443
PspN4I	GGNNCC	6 cut(s)	97, 342, 451, 803, 1523, 1691
PspOMI	GGGCCC	1 cut(s)	340
PspPI	GGNCC	7 cut(s)	238, 340, 341, 352, 450, 1165, 1522
PspPPI	RGGWCCY	2 cut(s)	450, 1522
PstI	CTGCAG	1 cut(s)	2178
PsuI	RGATCY	3 cut(s)	933, 1338, 2145
PsyI	GACNNNGTC	2 cut(s)	322, 742
PvuII	CAGCTG	2 cut(s)	2038, 2117
RsaI	GTAC	3 cut(s)	1751, 1816, 2219
RsaNI	GTAC	3 cut(s)	1750, 1815, 2218
RseI	CAYNNNNRTG	3 cut(s)	1445, 1665, 2022
SacI	GAGCTC	2 cut(s)	45, 2007
SapI	GCTCTTC	1 cut(s)	1536
SaqAI	TTAA	7 cut(s)	56, 455, 930, 1110, 1251, 1293, 2272
Sau96I	GGNCC	7 cut(s)	238, 340, 341, 352, 450, 1165, 1522
SchI	GAGTC	2 cut(s)	1238, 1276
ScrFI	CCNGG	3 cut(s)	428, 939, 1445
SduI	GDGCHC	3 cut(s)	45, 344, 2007
SfaNI	GCATC	3 cut(s)	979, 1444, 2240
SfcI	CTRYAG	2 cut(s)	2141, 2174
SinI	GGWCC	5 cut(s)	238, 352, 450, 1165, 1522
SmiMI	CAYNNNNRTG	3 cut(s)	1445, 1665, 2022
SmlI	CTYRAG	5 cut(s)	74, 914, 1867, 2069, 2077
SmoI	CTYRAG	5 cut(s)	74, 914, 1867, 2069, 2077
SspMI	CTAG	1 cut(s)	678
SstI	GAGCTC	2 cut(s)	45, 2007
StyD4I	CCNGG	3 cut(s)	426, 937, 1443
StyI	CCWWGG	1 cut(s)	2259
TaaI	ACNGT	6 cut(s)	322, 595, 742, 750, 865, 1814
TaiI	ACGT	7 cut(s)	285, 372, 967, 984, 1258, 1567, 2223
TatI	WGTACW	1 cut(s)	1814
TauI	GCSGC	5 cut(s)	65, 445, 922, 1082, 2171
TfiI	GAWTC	1 cut(s)	1622
Tru1I	TTAA	7 cut(s)	56, 455, 930, 1110, 1251, 1293, 2272
Tru9I	TTAA	7 cut(s)	56, 455, 930, 1110, 1251, 1293, 2272
TscAI	CASTG	9 cut(s)	12, 327, 747, 909, 915, 1549, 1558, 2178, 2240
TseFI	GTSAC	3 cut(s)	7, 744, 900
TseI	GCWGC	5 cut(s)	127, 942, 1123, 2038, 2131
Tsp45I	GTSAC	3 cut(s)	7, 744, 900
TspGWI	ACGGA	2 cut(s)	26, 53
TspRI	CASTG	9 cut(s)	12, 327, 747, 909, 915, 1549, 1558, 2178, 2240
Tth111I	GACNNNGTC	2 cut(s)	322, 742
Van91I	CCANNNNNTGG	2 cut(s)	361, 1365
VpaK11BI	GGWCC	5 cut(s)	238, 352, 450, 1165, 1522
XapI	RAATTY	8 cut(s)	52, 726, 807, 971, 1090, 1729, 1855, 2242
XceI	RCATGY	2 cut(s)	208, 1780
XspI	CTAG	1 cut(s)	678
ZraI	GACGTC	1 cut(s)	370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.