Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

2682933 .. 2686151

Gene Structure 3219 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g03440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGCTGTGACGGACTTCTTCGTCGGCGAGATCACGACGGAGCTCCTGAAAAATTTAATCGCCCTCTCCCGCAAGTCATGCAACAGCAGAGGAATCGCCGAGGAACTCAAAACCTCAGTCGAGCAGCTCCTCCCCATAATCCAAGAAATCAAGTACTCCGGCGTCGAGCTACCGGCTGAACGGCAATTCCAGCTCGACCGCATGTCGGAAGTTCTCCAGAAGGGCCTCGAACTGGTCCAAAAAATCCTCGCGACACCGCGCTGGAACTTTTACAGGCACGTCCGATTGGCGAAAAAGATGGAGGCTTTGGAGAAGACAGTGTCCAGATTTCTTCAGGGCCCATTACAGGTCCATATTTTGGCCGACGTCCACCACATTAGGGCGGGGATGGATCAGCGGTTCGACCGGCTTGATTGGTCGAACCGGATGCTGGAACAGCGGCTTGGGACCTTAAAGATTGGGACTTGCGGGGGCGGGGGTGTCGGTAGCGGGTGGATGGAGGAAGCTGTGAGGAGGGTGGAGGAGGAAGGGCAGATGAAGTGGGAGGTGAGCAGTTCGGTGGTGTTGGGATTGGGGGTGGGGATGAAGACGGTGAAGGGAATGATTTTTGGGAGGGAGGATTTGAGGGTCGTTGGAATTTGTGGGATTGGTGGCTCTGGAAAAACCACTCTCGCTAGAGAACTTTGCAAGGATGATCAAGTGAGATCTTATTTCAGTGAGAGAATTCTGTTCCTGACAGTATCACAGTCTCCAGATGTGAACCAGTTGAAGGCCAAGATTGTGCAGTTCATCATGGGGAACCAAATTCTCAGTCCCAATTCTGTGCTTCCCCAATGGAACTTGCAATATGAATGGATAACCACAACGAAAACTCTGGTGGTTTTGGATGATGTGTGGTCACTGGCAGTGCTTGAGCCGCTGCTCTTTAAGATCCCGGGCTGCAAGTTTCTTGTGGTCTCACGTTTCAAATTTCCAAACGTTCTCGATGCCACTTATGATGTTGAGTTGTTGAGGGAAGAAGAAGCACTCTCTCTGTTTTGTCACTCTGCTTTTGGCCAAAAGTCGATTCCCCCGGCTGCTGATAAAAATTTGGTCAAGCAGATTGTTAACGAGTGCAAGCGGCTTCCTTTGGCTCTCAAAGTGATTGGAGCTTCACTGAAGGACCAGCCTGAATTGTGTTGGGCAAATGCAAGGAGCAAGTTGTCGAAAGGGGAAGCTTTTTGTGAGTCCCATGAGAACAATCTACTTAAACGTATGGCAACGAGTCTCGACTGCTTATCCAAAAATGTTAAATCATGTTTCTTGGATTTGGGTGCGTTTCCAGAAGACAAGAAGATCCCTCTTGATGTCCTTACCAATATGTGGGTTGAAATACATGGCATCGACGAAGAAGAAGCTTTTGCTATTCTTGTCGAGCTTTCGAACAAAAATCTCCTCACCCTAGTGAAAGATGCACGAGTTGGAGACATATATAGCAGTTGCTATGACATATCTGTTACACAACATGATGTGCTGAGGGACCTTGCTCTTCATTTGAGCAATGATGGCAGCGTGAACGAACGCAGTCGATTGCTTATGCCAAGAAGAGAGAATGAACTTCCAAGAGAATGGGAGAGGGGTTCAGATCATCCATTTCACGCGAAGATTGTATCAGTTCATACTGGTGAAATGAAAGAGATGGACTGTTTCCAGATGGAGTTTCCCAAATGTGAAGTGCTGATTTTGAATTTTTCATCGGACGAGGAGTACCTTCTTCCTCCTTTCATTGAAAACATGCCAAAGTTGAGGGCATTGATAATCATGAATTACAGTACGTCAAATTGCATCCTAAATGGCTTTTCAGTCTTCTCAAACTTAACCAACTTGAGAAGCCTCTGGCTCGAAAAAGTATTTGTCCCTCAGTTATCTACCATCCCCCTGAAAAATCTGAGGAAAATCTATATAATCCTATGCAAGATCAATGTGGCCAATAGCCTTGATCAATCAGCCTTTCCTCGGCTCACAGAGCTCACCATCGATCATTGCGATGACTTATTCCAGCTGCCTTTATGCATTTGCGCAATGCCTTCTCTCAAGAACTTGAGCGTAACCAACTGCCACAGCCTATACCACCTCCCAGCTGATTTGGGAAAGCTGCACTCTCTACAGATCCTAAGGCTATATGCGTGTCCGGCACTGCAGACGCTTCCGGCCAGCATTTGCGAGTTACTTCGCTTGAAGTACATTGACATTTCCCAATGTGTAAATTTGAGATGCCTTCCCATGGACATTGGTAAGTTAGCAAGTTTGGAAAAGATTGAAATGAGGGAATGTTCACAAATTAGCAATCTACCGAAATCCGCTGCAACGTTACAGTCTCTGCGCCGTGTAGTGTGCGACGAGGATATTTCTTGGATGTGGAGAGACGCAGAGAAGGCATTGCCGGAGCTTCATGTTCAGGTGGTGGAGAAACATTTCGATTTGGACTGGCTCGACGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

827

Amino Acids

93.69

Weight (kDa)

6.13

Isoelectric Point (pI)

46.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	8 - 133	4e-12	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	196 - 354	1.1e-18	NB-ARC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	20
AatII	GACGTC	1 cut(s)	369
Acc16I	TGCGCA	1 cut(s)	2059
AccB7I	CCANNNNNTGG	2 cut(s)	358, 1362
AccII	CGCG	3 cut(s)	251, 259, 1640
AclI	AACGTT	2 cut(s)	978, 2348
AclWI	GGATC	4 cut(s)	399, 925, 1330, 2143
AcoI	YGGCCR	4 cut(s)	360, 1054, 1965, 2190
AcsI	RAATTY	8 cut(s)	52, 636, 723, 804, 968, 1087, 1726, 2245
AcuI	CTGAAG	2 cut(s)	317, 1178
AcyI	GRCGYC	2 cut(s)	162, 366
AfaI	GTAC	4 cut(s)	155, 1748, 1813, 2222
AgsI	TTSAA	7 cut(s)	769, 967, 1370, 1726, 1769, 2218, 2300
AhdI	GACNNNNNGTC	1 cut(s)	202
AjiI	CACGTC	1 cut(s)	280
AjuI	GAANNNNNNNTTGG	4 cut(s)	426, 458, 1050, 1082
AleI	CACNNNNGTG	1 cut(s)	1442
AloI	GAACNNNNNNTCC	4 cut(s)	171, 203, 383, 415
Alw21I	GWGCWC	2 cut(s)	45, 2010
Alw26I	GTCTC	6 cut(s)	753, 961, 1271, 1458, 2361, 2397
AlwI	GGATC	4 cut(s)	399, 925, 1330, 2143
AlwNI	CAGNNNCTG	1 cut(s)	2359
Ama87I	CYCGRG	1 cut(s)	934
AoxI	GGCC	7 cut(s)	223, 337, 360, 771, 1054, 1965, 2190
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	8 cut(s)	124, 919, 939, 1076, 1548, 2041, 2134, 2342
ApoI	RAATTY	8 cut(s)	52, 636, 723, 804, 968, 1087, 1726, 2245
ArsI	GACNNNNNNTTYG	4 cut(s)	102, 134, 796, 828
Asp700I	GAANNNNTTC	1 cut(s)	2453
AspLEI	GCGC	3 cut(s)	261, 2060, 2364
AspS9I	GGNCC	8 cut(s)	223, 235, 337, 338, 349, 447, 1162, 1519
AsuC2I	CCSGG	3 cut(s)	935, 936, 1073
AsuHPI	GGTGA	5 cut(s)	559, 604, 1429, 1676, 2002
AsuII	TTCGAA	1 cut(s)	1421
AvaI	CYCGRG	1 cut(s)	934
AvaII	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
AxyI	CCTNAGG	1 cut(s)	2153
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	2 cut(s)	1056, 1967
BanII	GRGCYC	3 cut(s)	45, 341, 2010
BauI	CACGAG	1 cut(s)	1455
BbsI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
Bbv12I	GWGCWC	2 cut(s)	45, 2010
BbvCI	CCTCAGC	1 cut(s)	1514
BbvI	GCAGC	8 cut(s)	136, 906, 926, 1063, 1560, 2028, 2121, 2329
BccI	CCATC	8 cut(s)	292, 382, 490, 1538, 1672, 1687, 1919, 2021
BceAI	ACGGC	2 cut(s)	197, 2349
BcgI	CGANNNNNNTGC	4 cut(s)	76, 110, 409, 443
BclI	TGATCA	2 cut(s)	694, 1978
BcnI	CCSGG	3 cut(s)	935, 936, 1073
BcoDI	GTCTC	6 cut(s)	753, 961, 1271, 1458, 2361, 2397
BfaI	CTAG	2 cut(s)	675, 1442
BfmI	CTRYAG	2 cut(s)	2144, 2177
BglII	AGATCT	1 cut(s)	704
BmcAI	AGTACT	1 cut(s)	155
Bme1390I	CCNGG	3 cut(s)	935, 936, 1073
Bme18I	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
BmeRI	GACNNNNNGTC	1 cut(s)	202
BmeT110I	CYCGRG	1 cut(s)	934
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	8 cut(s)	223, 235, 337, 338, 349, 447, 1162, 1519
BmiI	GGNNCC	4 cut(s)	339, 448, 800, 1520
BmrFI	CCNGG	3 cut(s)	935, 936, 1073
BmsI	GCATC	6 cut(s)	417, 976, 1389, 1441, 1833, 2243
BpiI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BpmI	CTGGAG	2 cut(s)	200, 735
Bpu10I	CCTNAGC	1 cut(s)	1514
Bpu14I	TTCGAA	1 cut(s)	1421
BpuEI	CTTGAG	4 cut(s)	932, 1885, 2057, 2101
BpuMI	CCSGG	3 cut(s)	935, 936, 1073
Bsa29I	ATCGAT	1 cut(s)	2016
BsaHI	GRCGYC	2 cut(s)	162, 366
BsaI	GGTCTC	1 cut(s)	961
BsaJI	CCNNGG	5 cut(s)	99, 934, 1071, 1994, 2262
BsaWI	WCCGGW	1 cut(s)	423
Bse118I	RCCGGY	2 cut(s)	172, 405
Bse1I	ACTGG	5 cut(s)	237, 763, 906, 1666, 2471
Bse21I	CCTNAGG	1 cut(s)	2153
Bse3DI	GCAATG	4 cut(s)	1546, 2020, 2067, 2417
BseCI	ATCGAT	1 cut(s)	2016
BseDI	CCNNGG	5 cut(s)	99, 934, 1071, 1994, 2262
BseMI	GCAATG	4 cut(s)	1546, 2020, 2067, 2417
BseMII	CTCAG	5 cut(s)	129, 823, 1505, 1913, 1919
BseNI	ACTGG	5 cut(s)	237, 763, 906, 1666, 2471
BseRI	GAGGAG	5 cut(s)	119, 526, 536, 1424, 1757
BseSI	GKGCMC	1 cut(s)	341
BseXI	GCAGC	8 cut(s)	136, 906, 926, 1063, 1560, 2028, 2121, 2329
BseYI	CCCAGC	1 cut(s)	2116
BsgI	GTGCAG	2 cut(s)	803, 2120
Bsh1236I	CGCG	3 cut(s)	251, 259, 1640
Bsh1285I	CGRYCG	2 cut(s)	199, 406
BshFI	GGCC	7 cut(s)	225, 339, 362, 773, 1056, 1967, 2192
BshVI	ATCGAT	1 cut(s)	2016
BsiEI	CGRYCG	2 cut(s)	199, 406
BsiHKAI	GWGCWC	2 cut(s)	45, 2010
BsiHKCI	CYCGRG	1 cut(s)	934
BsiSI	CCGG	9 cut(s)	159, 173, 406, 424, 935, 1073, 2171, 2189, 2423
BslFI	GGGAC	6 cut(s)	460, 475, 798, 1213, 1532, 1880
BsmAI	GTCTC	6 cut(s)	753, 961, 1271, 1458, 2361, 2397
BsmBI	CGTCTC	1 cut(s)	2397
BsmFI	GGGAC	6 cut(s)	460, 475, 798, 1213, 1532, 1880
BsnI	GGCC	7 cut(s)	225, 339, 362, 773, 1056, 1967, 2192
Bso31I	GGTCTC	1 cut(s)	961
BsoBI	CYCGRG	1 cut(s)	934
Bsp119I	TTCGAA	1 cut(s)	1421
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	3 cut(s)	45, 341, 2010
Bsp19I	CCATGG	1 cut(s)	2262
Bsp68I	TCGCGA	1 cut(s)	251
BspANI	GGCC	7 cut(s)	225, 339, 362, 773, 1056, 1967, 2192
BspCNI	CTCAG	5 cut(s)	128, 822, 1506, 1912, 1920
BspDI	ATCGAT	1 cut(s)	2016
BspFNI	CGCG	3 cut(s)	251, 259, 1640
BspHI	TCATGA	1 cut(s)	1800
BspLI	GGNNCC	4 cut(s)	339, 448, 800, 1520
BspMAI	CTGCAG	1 cut(s)	2181
BspPI	GGATC	4 cut(s)	399, 925, 1330, 2143
BspQI	GCTCTTC	1 cut(s)	1533
BspT104I	TTCGAA	1 cut(s)	1421
BspTNI	GGTCTC	1 cut(s)	961
BsrDI	GCAATG	4 cut(s)	1546, 2020, 2067, 2417
BsrFI	RCCGGY	2 cut(s)	172, 405
BsrI	ACTGG	5 cut(s)	237, 763, 906, 1666, 2471
BssAI	RCCGGY	2 cut(s)	172, 405
BssECI	CCNNGG	5 cut(s)	99, 934, 1071, 1994, 2262
BssNI	GRCGYC	2 cut(s)	162, 366
BssSI	CACGAG	1 cut(s)	1455
BssT1I	CCWWGG	1 cut(s)	2262
Bst2BI	CACGAG	1 cut(s)	1455
Bst4CI	ACNGT	7 cut(s)	319, 592, 739, 747, 1685, 1811, 2355
Bst6I	CTCTTC	2 cut(s)	1533, 1579
BstACI	GRCGYC	2 cut(s)	162, 366
BstAPI	GCANNNNNTGC	1 cut(s)	78
BstBI	TTCGAA	1 cut(s)	1421
BstC8I	GCNNGC	2 cut(s)	1118, 2194
BstDEI	CTNAG	6 cut(s)	115, 809, 1514, 1899, 1928, 2153
BstDSI	CCRYGG	1 cut(s)	2262
BstFNI	CGCG	3 cut(s)	251, 259, 1640
BstHHI	GCGC	3 cut(s)	261, 2060, 2364
BstMAI	GTCTC	6 cut(s)	753, 961, 1271, 1458, 2361, 2397
BstMCI	CGRYCG	2 cut(s)	199, 406
BstMWI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 2005, 2171, 2414
BstNSI	RCATGY	2 cut(s)	205, 1777
BstSCI	CCNGG	3 cut(s)	933, 934, 1071
BstSFI	CTRYAG	2 cut(s)	2144, 2177
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	3 cut(s)	251, 259, 1640
BstV1I	GCAGC	8 cut(s)	136, 906, 926, 1063, 1560, 2028, 2121, 2329
BstV2I	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BstX2I	RGATCY	4 cut(s)	704, 930, 1335, 2148
BstXI	CCANNNNNNTGG	1 cut(s)	1608
BstYI	RGATCY	4 cut(s)	704, 930, 1335, 2148
Bsu15I	ATCGAT	1 cut(s)	2016
Bsu36I	CCTNAGG	1 cut(s)	2153
BsuRI	GGCC	7 cut(s)	225, 339, 362, 773, 1056, 1967, 2192
BsuTUI	ATCGAT	1 cut(s)	2016
BtgI	CCRYGG	1 cut(s)	2262
BtgZI	GCGATG	1 cut(s)	2040
BtrI	CACGTC	1 cut(s)	280
BtsI	GCAGTG	2 cut(s)	912, 2174
BtsIMutI	CAGTG	6 cut(s)	324, 721, 899, 912, 1154, 2174
BtuMI	TCGCGA	1 cut(s)	251
Cac8I	GCNNGC	2 cut(s)	1118, 2194
CaiI	CAGNNNCTG	1 cut(s)	2359
CciI	TCATGA	1 cut(s)	1800
CfoI	GCGC	3 cut(s)	261, 2060, 2364
Cfr10I	RCCGGY	2 cut(s)	172, 405
Cfr13I	GGNCC	8 cut(s)	223, 235, 337, 338, 349, 447, 1162, 1519
Cfr9I	CCCGGG	1 cut(s)	934
ClaI	ATCGAT	1 cut(s)	2016
CseI	GACGC	3 cut(s)	151, 2191, 2414
Csp6I	GTAC	4 cut(s)	154, 1747, 1812, 2221
CviQI	GTAC	4 cut(s)	154, 1747, 1812, 2221
DdeI	CTNAG	6 cut(s)	115, 809, 1514, 1899, 1928, 2153
DrdI	GACNNNNNNGTC	1 cut(s)	20
DriI	GACNNNNNGTC	1 cut(s)	202
DseDI	GACNNNNNNGTC	1 cut(s)	20
EaeI	YGGCCR	4 cut(s)	360, 1054, 1965, 2190
Eam1104I	CTCTTC	2 cut(s)	1533, 1579
Eam1105I	GACNNNNNGTC	1 cut(s)	202
EarI	CTCTTC	2 cut(s)	1533, 1579
Ecl136II	GAGCTC	2 cut(s)	43, 2008
Eco130I	CCWWGG	1 cut(s)	2262
Eco24I	GRGCYC	3 cut(s)	45, 341, 2010
Eco31I	GGTCTC	1 cut(s)	961
Eco47I	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
Eco53kI	GAGCTC	2 cut(s)	43, 2008
Eco57I	CTGAAG	2 cut(s)	317, 1178
Eco81I	CCTNAGG	1 cut(s)	2153
Eco88I	CYCGRG	1 cut(s)	934
EcoICRI	GAGCTC	2 cut(s)	43, 2008
EcoO109I	RGGNCCY	4 cut(s)	223, 337, 447, 1519
EcoRI	GAATTC	1 cut(s)	723
EcoT14I	CCWWGG	1 cut(s)	2262
EcoT22I	ATGCAT	1 cut(s)	2054
EcoT38I	GRGCYC	3 cut(s)	45, 341, 2010
ErhI	CCWWGG	1 cut(s)	2262
Esp3I	CGTCTC	1 cut(s)	2397
FaqI	GGGAC	6 cut(s)	460, 475, 798, 1213, 1532, 1880
FauI	CCCGC	5 cut(s)	77, 376, 461, 467, 482
FbaI	TGATCA	2 cut(s)	694, 1978
FriOI	GRGCYC	3 cut(s)	45, 341, 2010
FspBI	CTAG	2 cut(s)	675, 1442
FspI	TGCGCA	1 cut(s)	2059
GlaI	GCGC	3 cut(s)	260, 2059, 2363
GsaI	CCCAGC	1 cut(s)	2120
GsuI	CTGGAG	2 cut(s)	200, 735
HaeIII	GGCC	7 cut(s)	225, 339, 362, 773, 1056, 1967, 2192
HapII	CCGG	9 cut(s)	159, 173, 406, 424, 935, 1073, 2171, 2189, 2423
HgaI	GACGC	3 cut(s)	151, 2191, 2414
HhaI	GCGC	3 cut(s)	261, 2060, 2364
Hin1I	GRCGYC	2 cut(s)	162, 366
Hin6I	GCGC	3 cut(s)	259, 2058, 2362
HinP1I	GCGC	3 cut(s)	259, 2058, 2362
HincII	GTYRAC	1 cut(s)	1108
HindII	GTYRAC	1 cut(s)	1108
HindIII	AAGCTT	2 cut(s)	1215, 1395
HinfI	GANTC	4 cut(s)	93, 1066, 1226, 1264
HpaI	GTTAAC	1 cut(s)	1108
HpaII	CCGG	9 cut(s)	159, 173, 406, 424, 935, 1073, 2171, 2189, 2423
HphI	GGTGA	5 cut(s)	559, 604, 1429, 1676, 2002
Hpy166II	GTNNAC	5 cut(s)	370, 760, 1108, 1555, 2315
Hpy188I	TCNGA	5 cut(s)	208, 284, 1624, 1738, 1929
Hpy8I	GTNNAC	5 cut(s)	370, 760, 1108, 1555, 2315
Hpy99I	CGWCG	7 cut(s)	26, 40, 167, 368, 1388, 2381, 2477
HpyAV	CCTTC	9 cut(s)	214, 521, 589, 763, 1153, 1760, 2076, 2267, 2407
HpyCH4III	ACNGT	7 cut(s)	319, 592, 739, 747, 1685, 1811, 2355
HpyCH4IV	ACGT	7 cut(s)	279, 366, 961, 978, 1252, 1814, 2348
HpyF10VI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 2005, 2171, 2414
HpyF3I	CTNAG	6 cut(s)	115, 809, 1514, 1899, 1928, 2153
HpySE526I	ACGT	7 cut(s)	279, 366, 961, 978, 1252, 1814, 2348
Hsp92I	GRCGYC	2 cut(s)	162, 366
HspAI	GCGC	3 cut(s)	259, 2058, 2362
Ksp22I	TGATCA	2 cut(s)	694, 1978
KspAI	GTTAAC	1 cut(s)	1108
LguI	GCTCTTC	1 cut(s)	1533
LmnI	GCTCC	6 cut(s)	40, 48, 132, 1148, 1194, 2425
Lsp1109I	GCAGC	8 cut(s)	136, 906, 926, 1063, 1560, 2028, 2121, 2329
LweI	GCATC	6 cut(s)	417, 976, 1389, 1441, 1833, 2243
MaeI	CTAG	2 cut(s)	675, 1442
MaeII	ACGT	7 cut(s)	279, 366, 961, 978, 1252, 1814, 2348
MaeIII	GTNAC	7 cut(s)	7, 897, 1040, 1495, 2086, 2205, 2349
MflI	RGATCY	4 cut(s)	704, 930, 1335, 2148
MhlI	GDGCHC	3 cut(s)	45, 341, 2010
MlsI	TGGCCA	2 cut(s)	1056, 1967
MluNI	TGGCCA	2 cut(s)	1056, 1967
MlyI	GAGTC	2 cut(s)	1235, 1273
MmeI	TCCRAC	3 cut(s)	186, 613, 1441
Mox20I	TGGCCA	2 cut(s)	1056, 1967
Mph1103I	ATGCAT	1 cut(s)	2054
MroXI	GAANNNNTTC	1 cut(s)	2453
MscI	TGGCCA	2 cut(s)	1056, 1967
MseI	TTAA	7 cut(s)	56, 452, 927, 1107, 1248, 1290, 1856
MslI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2025
Msp20I	TGGCCA	2 cut(s)	1056, 1967
MspA1I	CMGCKG	6 cut(s)	397, 439, 919, 2041, 2120, 2342
MspI	CCGG	9 cut(s)	159, 173, 406, 424, 935, 1073, 2171, 2189, 2423
MspR9I	CCNGG	3 cut(s)	935, 936, 1073
MvnI	CGCG	3 cut(s)	251, 259, 1640
MwoI	GCNNNNNNNGC	8 cut(s)	78, 190, 436, 916, 1545, 2005, 2171, 2414
NciI	CCSGG	3 cut(s)	935, 936, 1073
NcoI	CCATGG	1 cut(s)	2262
NlaIV	GGNNCC	4 cut(s)	339, 448, 800, 1520
NmeAIII	GCCGAG	2 cut(s)	124, 1975
NmuCI	GTSAC	3 cut(s)	7, 897, 1040
NruI	TCGCGA	1 cut(s)	251
NsbI	TGCGCA	1 cut(s)	2059
NsiI	ATGCAT	1 cut(s)	2054
NspI	RCATGY	2 cut(s)	205, 1777
NspV	TTCGAA	1 cut(s)	1421
OliI	CACNNNNGTG	1 cut(s)	1442
PagI	TCATGA	1 cut(s)	1800
PciSI	GCTCTTC	1 cut(s)	1533
PcsI	WCGNNNNNNNCGW	1 cut(s)	2022
PdmI	GAANNNNTTC	1 cut(s)	2453
PfeI	GAWTC	2 cut(s)	93, 1066
PflFI	GACNNNGTC	1 cut(s)	319
PflMI	CCANNNNNTGG	2 cut(s)	358, 1362
PleI	GAGTC	2 cut(s)	1234, 1272
PpsI	GAGTC	2 cut(s)	1234, 1272
PpuMI	RGGWCCY	2 cut(s)	447, 1519
Psp124BI	GAGCTC	2 cut(s)	45, 2010
Psp1406I	AACGTT	2 cut(s)	978, 2348
Psp5II	RGGWCCY	2 cut(s)	447, 1519
PspFI	CCCAGC	1 cut(s)	2116
PspN4I	GGNNCC	4 cut(s)	339, 448, 800, 1520
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	8 cut(s)	223, 235, 337, 338, 349, 447, 1162, 1519
PspPPI	RGGWCCY	2 cut(s)	447, 1519
PstI	CTGCAG	1 cut(s)	2181
PstNI	CAGNNNCTG	1 cut(s)	2359
PsuI	RGATCY	4 cut(s)	704, 930, 1335, 2148
PsyI	GACNNNGTC	1 cut(s)	319
PvuII	CAGCTG	2 cut(s)	2041, 2120
RruI	TCGCGA	1 cut(s)	251
RsaI	GTAC	4 cut(s)	155, 1748, 1813, 2222
RsaNI	GTAC	4 cut(s)	154, 1747, 1812, 2221
RseI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2025
SacI	GAGCTC	2 cut(s)	45, 2010
SapI	GCTCTTC	1 cut(s)	1533
SaqAI	TTAA	7 cut(s)	56, 452, 927, 1107, 1248, 1290, 1856
Sau96I	GGNCC	8 cut(s)	223, 235, 337, 338, 349, 447, 1162, 1519
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	2 cut(s)	1235, 1273
ScrFI	CCNGG	3 cut(s)	935, 936, 1073
SduI	GDGCHC	3 cut(s)	45, 341, 2010
SfaNI	GCATC	6 cut(s)	417, 976, 1389, 1441, 1833, 2243
SfcI	CTRYAG	2 cut(s)	2144, 2177
SfuI	TTCGAA	1 cut(s)	1421
SinI	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
SmaI	CCCGGG	1 cut(s)	936
SmiMI	CAYNNNNRTG	3 cut(s)	1442, 1662, 2025
SmlI	CTYRAG	4 cut(s)	911, 1864, 2072, 2080
SmoI	CTYRAG	4 cut(s)	911, 1864, 2072, 2080
SspMI	CTAG	2 cut(s)	675, 1442
SstI	GAGCTC	2 cut(s)	45, 2010
StyD4I	CCNGG	3 cut(s)	933, 934, 1071
StyI	CCWWGG	1 cut(s)	2262
TaaI	ACNGT	7 cut(s)	319, 592, 739, 747, 1685, 1811, 2355
TaiI	ACGT	7 cut(s)	282, 369, 964, 981, 1255, 1817, 2351
TatI	WGTACW	2 cut(s)	153, 2220
TauI	GCSGC	3 cut(s)	442, 919, 1123
TfiI	GAWTC	2 cut(s)	93, 1066
Tru1I	TTAA	7 cut(s)	56, 452, 927, 1107, 1248, 1290, 1856
Tru9I	TTAA	7 cut(s)	56, 452, 927, 1107, 1248, 1290, 1856
TscAI	CASTG	6 cut(s)	324, 721, 906, 912, 1161, 2181
TseFI	GTSAC	3 cut(s)	7, 897, 1040
TseI	GCWGC	8 cut(s)	124, 919, 939, 1076, 1548, 2041, 2134, 2342
Tsp45I	GTSAC	3 cut(s)	7, 897, 1040
TspGWI	ACGGA	2 cut(s)	26, 53
TspMI	CCCGGG	1 cut(s)	934
TspRI	CASTG	6 cut(s)	324, 721, 906, 912, 1161, 2181
Tth111I	GACNNNGTC	1 cut(s)	319
Van91I	CCANNNNNTGG	2 cut(s)	358, 1362
VpaK11BI	GGWCC	5 cut(s)	235, 349, 447, 1162, 1519
XapI	RAATTY	8 cut(s)	52, 636, 723, 804, 968, 1087, 1726, 2245
XceI	RCATGY	2 cut(s)	205, 1777
XmaI	CCCGGG	1 cut(s)	934
XmnI	GAANNNNTTC	1 cut(s)	2453
XspI	CTAG	2 cut(s)	675, 1442
ZraI	GACGTC	1 cut(s)	367
ZrmI	AGTACT	1 cut(s)	155
Zsp2I	ATGCAT	1 cut(s)	2054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.