Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

2623467 .. 2625562

Gene Structure 2096 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g03380.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGGGACCCAAATTCTCAGTCCCAATTCTGTGCTTCCCCAATGGAACTTGCAGTATGAATGGATAACCGTAACGAAATCTCTGGTGGTTTTGGATGATGTGTGGTCACTGGCAGTGCTTGAGCCGCTGCTCCTTAAGATCGCGGGCTGCAAGTTTCTTGTGGTCTCACGTTTCAAATTCCCAAACGTTCTCGATGCCACTTATGATGTTGAGTTGTTGAGGGAAGAAGAAGCACTCTCTCTGTTTTGTCACTCTGCTTTTGGCCAAAAGTCCATTCCTCCTGCGGCTGATAAAAATTTGGTCAAGCAGATTGTTAACGAGTGCAAGCGGCTTCCTTTGGCTCTCAAAGTGATTGGAGCTTCATTGAAGGACCAGCCTGAATTGTGTTGGGCAAATGCAAGGAGCAAGTTGTCAAAAGGGGAAGCTTTTTGTGAGTCCCATGAGAACAATCTACTTCAGCGAATGGCAGCGAGTCTCGACTGCTTATCCAAAAAAGTTAAATCATGTTTCTTAGATTTGGGTGCGTTTCCAGAAGACAAGAAGATCCCTCTTGATATCCTTACCAATATGTGGGTTGAAATACATGGCATCGATGAAGAAGAAGCGTTTGCTATACTTGTCGAGCTTTCGAACAAAAATCTCCTCACCCTGGTGAAAGATGCAAGAGATGGAGACATATATAGCAGTTGCTATGACATATCTGTTACACAACATGATGTGCTGAGGGACCTTGCTCTTCATTTGAGCAGTGATGGCAGCGTGAACGAACGTAGTCGATTGCTTATGCCAAGAAGAGAGACTGAACTTCCAAGAGAATGGGAGAGGGATTCAGATCAGCCATTTCACGCGAAGATTGTATCAGTTCATACCGGTGAAATGAGAGAGATGGACTGGTTCCAGATGGAGTTTCCCAAATGTGAAGTGCTGATTTTGAATTTTTCATCGGACGACGAGTACCTTCTTCCTCCTTTCATTGAAAACATGCCAAAGTTGAGGGCATTGATAATCATGAATTACAGTACATCAAATTGCATCCTAAATGGCTTTTCAGTCTTCTCAAACTTAACCAACTTGAGAAGCCTCTGGCTCGAAAAAGTATTTGTCCCTCGATTATCTACCATCCCCCTGAAAAATCTGAAGAAAATCTATATAATCCTATGCAAGATAAGTAATAGCCTTGATCAATCGGCCTTTCCTCGGCTCACAGAGCTCACCATCGATCATTGCGATGACTTGTTCCAGCTGCCTTCGTGCATTTACGCAATGCCTTCGCTCAAGAACTTGAGCGTAACCAACTGCCACAGCCTATACCACCTCCCAGCTGATTTGGGAAGGCTGCAATCTCTACAGATCCTAAGGCTATACGCGTGTCCGGCACTGCAGACGCTTCCGGCCAGCATTTGCGAGCTACTTCGCTTGAAGTACATTGACATTTCCCAATGTGTAAATTTGAGATGCCTACCCATGGACATTGGTAAGCTAGCAAGTTTGGAAAAGATTGACATGAGGGAATGTTCACAAATTAGCAATCTGCCGAAATCCGCTGCAGCGTTACAGTCTCTGCGCCGTGTAGTGTGCGACGAGGATATTTCTTGGATGTGGAGAGATGCAGAGATGGCATTGCCGGAGCTTCATGTTCAGGTGGTGGAGAAACATTTCGATCTGGACTGGCTCGATGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

561

Amino Acids

63.86

Weight (kDa)

5.34

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	570
AccII	CGCG	3 cut(s)	143, 848, 1367
AciI	CCGC	5 cut(s)	125, 143, 284, 328, 1542
AclI	AACGTT	1 cut(s)	186
AclWI	GGATC	2 cut(s)	538, 1345
AcoI	YGGCCR	2 cut(s)	262, 1392
AcsI	RAATTY	5 cut(s)	12, 176, 295, 934, 1447
AcuI	CTGAAG	2 cut(s)	440, 1157
AfaI	GTAC	3 cut(s)	956, 1021, 1424
AfiI	CCNNNNNNNGG	4 cut(s)	570, 649, 1197, 1465
AflII	CTTAAG	1 cut(s)	134
AflIII	ACRYGT	1 cut(s)	1365
AgeI	ACCGGT	1 cut(s)	869
AgsI	TTSAA	6 cut(s)	175, 367, 578, 934, 977, 1420
AjnI	CCWGG	1 cut(s)	648
AjuI	GAANNNNNNNTTGG	2 cut(s)	258, 290
AleI	CACNNNNGTG	1 cut(s)	650
AluBI	AGCT	9 cut(s)	359, 425, 625, 1210, 1243, 1322, 1408, 1480, 1630
AluI	AGCT	9 cut(s)	359, 425, 625, 1210, 1243, 1322, 1408, 1480, 1630
Alw21I	GWGCWC	1 cut(s)	1212
Alw26I	GTCTC	5 cut(s)	169, 479, 666, 791, 1563
AlwI	GGATC	2 cut(s)	538, 1345
AlwNI	CAGNNNCTG	1 cut(s)	1561
AoxI	GGCC	3 cut(s)	262, 1188, 1392
ApeKI	GCWGC	8 cut(s)	127, 147, 467, 756, 1243, 1336, 1544, 1547
ApoI	RAATTY	5 cut(s)	12, 176, 295, 934, 1447
ArsI	GACNNNNNNTTYG	1 cut(s)	36
AsiGI	ACCGGT	1 cut(s)	869
Asp700I	GAANNNNTTC	1 cut(s)	1655
AspLEI	GCGC	1 cut(s)	1566
AspS9I	GGNCC	3 cut(s)	6, 370, 727
AsuHPI	GGTGA	4 cut(s)	637, 664, 884, 1204
AsuII	TTCGAA	1 cut(s)	629
AsuNHI	GCTAGC	1 cut(s)	1480
AvaII	GGWCC	3 cut(s)	6, 370, 727
AxyI	CCTNAGG	1 cut(s)	1355
BalI	TGGCCA	1 cut(s)	264
BanII	GRGCYC	1 cut(s)	1212
BbsI	GAAGAC	2 cut(s)	540, 1045
Bbv12I	GWGCWC	1 cut(s)	1212
BbvCI	CCTCAGC	1 cut(s)	722
BbvI	GCAGC	8 cut(s)	114, 134, 479, 768, 1230, 1323, 1531, 1559
BccI	CCATC	7 cut(s)	662, 746, 880, 895, 1127, 1223, 1609
BceAI	ACGGC	1 cut(s)	1551
BcgI	CGANNNNNNTGC	2 cut(s)	1251, 1285
BciT130I	CCWGG	1 cut(s)	650
BclI	TGATCA	1 cut(s)	1180
BcoDI	GTCTC	5 cut(s)	169, 479, 666, 791, 1563
BfaI	CTAG	1 cut(s)	1481
BfmI	CTRYAG	3 cut(s)	1346, 1379, 1545
BfrI	CTTAAG	1 cut(s)	134
Bme1390I	CCNGG	1 cut(s)	650
Bme18I	GGWCC	3 cut(s)	6, 370, 727
BmgT120I	GGNCC	3 cut(s)	6, 370, 727
BmiI	GGNNCC	4 cut(s)	7, 8, 728, 896
BmrFI	CCNGG	1 cut(s)	650
BmsI	GCATC	6 cut(s)	184, 597, 649, 1041, 1445, 1597
BmtI	GCTAGC	1 cut(s)	1484
BpiI	GAAGAC	2 cut(s)	540, 1045
Bpu10I	CCTNAGC	1 cut(s)	722
Bpu14I	TTCGAA	1 cut(s)	629
BpuEI	CTTGAG	4 cut(s)	140, 1093, 1259, 1303
Bsa29I	ATCGAT	2 cut(s)	591, 1218
BsaBI	GATNNNNATC	1 cut(s)	831
BsaI	GGTCTC	1 cut(s)	169
BsaJI	CCNNGG	3 cut(s)	648, 1196, 1464
BsaWI	WCCGGW	1 cut(s)	869
Bsc4I	CCNNNNNNNGG	4 cut(s)	570, 649, 1197, 1465
Bse118I	RCCGGY	1 cut(s)	869
Bse1I	ACTGG	3 cut(s)	114, 896, 1673
Bse21I	CCTNAGG	1 cut(s)	1355
Bse3DI	GCAATG	3 cut(s)	1222, 1269, 1619
Bse8I	GATNNNNATC	1 cut(s)	831
BseBI	CCWGG	1 cut(s)	650
BseCI	ATCGAT	2 cut(s)	591, 1218
BseDI	CCNNGG	3 cut(s)	648, 1196, 1464
BseGI	GGATG	4 cut(s)	100, 1032, 1119, 1602
BseJI	GATNNNNATC	1 cut(s)	831
BseLI	CCNNNNNNNGG	4 cut(s)	570, 649, 1197, 1465
BseMI	GCAATG	3 cut(s)	1222, 1269, 1619
BseMII	CTCAG	2 cut(s)	31, 713
BseNI	ACTGG	3 cut(s)	114, 896, 1673
BseRI	GAGGAG	1 cut(s)	632
BseXI	GCAGC	8 cut(s)	114, 134, 479, 768, 1230, 1323, 1531, 1559
BseYI	CCCAGC	1 cut(s)	1318
Bsh1236I	CGCG	3 cut(s)	143, 848, 1367
BshFI	GGCC	3 cut(s)	264, 1190, 1394
BshTI	ACCGGT	1 cut(s)	869
BshVI	ATCGAT	2 cut(s)	591, 1218
BsiHKAI	GWGCWC	1 cut(s)	1212
BsiSI	CCGG	4 cut(s)	870, 1373, 1391, 1625
BslFI	GGGAC	5 cut(s)	6, 19, 421, 740, 1088
BslI	CCNNNNNNNGG	4 cut(s)	570, 649, 1197, 1465
BsmAI	GTCTC	5 cut(s)	169, 479, 666, 791, 1563
BsmFI	GGGAC	5 cut(s)	6, 19, 421, 740, 1088
BsnI	GGCC	3 cut(s)	264, 1190, 1394
Bso31I	GGTCTC	1 cut(s)	169
Bsp119I	TTCGAA	1 cut(s)	629
Bsp1286I	GDGCHC	1 cut(s)	1212
Bsp143I	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
Bsp19I	CCATGG	1 cut(s)	1464
BspACI	CCGC	5 cut(s)	125, 143, 284, 328, 1542
BspANI	GGCC	3 cut(s)	264, 1190, 1394
BspCNI	CTCAG	2 cut(s)	30, 714
BspDI	ATCGAT	2 cut(s)	591, 1218
BspFNI	CGCG	3 cut(s)	143, 848, 1367
BspHI	TCATGA	1 cut(s)	1008
BspLI	GGNNCC	4 cut(s)	7, 8, 728, 896
BspMAI	CTGCAG	2 cut(s)	1383, 1549
BspOI	GCTAGC	1 cut(s)	1484
BspPI	GGATC	2 cut(s)	538, 1345
BspQI	GCTCTTC	1 cut(s)	741
BspT104I	TTCGAA	1 cut(s)	629
BspTI	CTTAAG	1 cut(s)	134
BspTNI	GGTCTC	1 cut(s)	169
BsrDI	GCAATG	3 cut(s)	1222, 1269, 1619
BsrFI	RCCGGY	1 cut(s)	869
BsrI	ACTGG	3 cut(s)	114, 896, 1673
BssAI	RCCGGY	1 cut(s)	869
BssECI	CCNNGG	3 cut(s)	648, 1196, 1464
BssMI	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
BssT1I	CCWWGG	1 cut(s)	1464
Bst2UI	CCWGG	1 cut(s)	650
Bst4CI	ACNGT	3 cut(s)	70, 1019, 1557
Bst6I	CTCTTC	2 cut(s)	741, 787
BstAFI	CTTAAG	1 cut(s)	134
BstBI	TTCGAA	1 cut(s)	629
BstC8I	GCNNGC	5 cut(s)	145, 326, 1396, 1406, 1482
BstDEI	CTNAG	4 cut(s)	17, 511, 722, 1355
BstDSI	CCRYGG	1 cut(s)	1464
BstF5I	GGATG	4 cut(s)	100, 1032, 1119, 1602
BstFNI	CGCG	3 cut(s)	143, 848, 1367
BstHHI	GCGC	1 cut(s)	1566
BstKTI	GATC	7 cut(s)	141, 546, 835, 1183, 1222, 1353, 1663
BstMAI	GTCTC	5 cut(s)	169, 479, 666, 791, 1563
BstMBI	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
BstMWI	GCNNNNNNNGC	5 cut(s)	124, 753, 1207, 1373, 1616
BstNI	CCWGG	1 cut(s)	650
BstNSI	RCATGY	1 cut(s)	985
BstSCI	CCNGG	1 cut(s)	648
BstSFI	CTRYAG	3 cut(s)	1346, 1379, 1545
BstUI	CGCG	3 cut(s)	143, 848, 1367
BstV1I	GCAGC	8 cut(s)	114, 134, 479, 768, 1230, 1323, 1531, 1559
BstV2I	GAAGAC	2 cut(s)	540, 1045
BstX2I	RGATCY	2 cut(s)	543, 1350
BstXI	CCANNNNNNTGG	1 cut(s)	816
BstYI	RGATCY	2 cut(s)	543, 1350
Bsu15I	ATCGAT	2 cut(s)	591, 1218
Bsu36I	CCTNAGG	1 cut(s)	1355
BsuRI	GGCC	3 cut(s)	264, 1190, 1394
BsuTUI	ATCGAT	2 cut(s)	591, 1218
BtgI	CCRYGG	1 cut(s)	1464
BtgZI	GCGATG	1 cut(s)	1242
BtsCI	GGATG	4 cut(s)	100, 1032, 1119, 1602
BtsI	GCAGTG	3 cut(s)	120, 754, 1376
BtsIMutI	CAGTG	4 cut(s)	107, 120, 754, 1376
Cac8I	GCNNGC	5 cut(s)	145, 326, 1396, 1406, 1482
CaiI	CAGNNNCTG	1 cut(s)	1561
CciI	TCATGA	1 cut(s)	1008
CfoI	GCGC	1 cut(s)	1566
Cfr10I	RCCGGY	1 cut(s)	869
Cfr13I	GGNCC	3 cut(s)	6, 370, 727
ClaI	ATCGAT	2 cut(s)	591, 1218
CseI	GACGC	1 cut(s)	1393
Csp6I	GTAC	3 cut(s)	955, 1020, 1423
CspAI	ACCGGT	1 cut(s)	869
CviAII	CATG	9 cut(s)	440, 504, 584, 713, 982, 1009, 1465, 1504, 1634
CviQI	GTAC	3 cut(s)	955, 1020, 1423
DdeI	CTNAG	4 cut(s)	17, 511, 722, 1355
DpnI	GATC	7 cut(s)	140, 545, 834, 1182, 1221, 1352, 1662
DpnII	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
EaeI	YGGCCR	2 cut(s)	262, 1392
Eam1104I	CTCTTC	2 cut(s)	741, 787
EarI	CTCTTC	2 cut(s)	741, 787
Ecl136II	GAGCTC	1 cut(s)	1210
Eco130I	CCWWGG	1 cut(s)	1464
Eco24I	GRGCYC	1 cut(s)	1212
Eco31I	GGTCTC	1 cut(s)	169
Eco32I	GATATC	1 cut(s)	556
Eco47I	GGWCC	3 cut(s)	6, 370, 727
Eco53kI	GAGCTC	1 cut(s)	1210
Eco57I	CTGAAG	2 cut(s)	440, 1157
Eco81I	CCTNAGG	1 cut(s)	1355
EcoICRI	GAGCTC	1 cut(s)	1210
EcoO109I	RGGNCCY	2 cut(s)	6, 727
EcoRII	CCWGG	1 cut(s)	648
EcoRV	GATATC	1 cut(s)	556
EcoT14I	CCWWGG	1 cut(s)	1464
EcoT38I	GRGCYC	1 cut(s)	1212
ErhI	CCWWGG	1 cut(s)	1464
FaeI	CATG	9 cut(s)	443, 507, 587, 716, 985, 1012, 1468, 1507, 1637
FaqI	GGGAC	5 cut(s)	6, 19, 421, 740, 1088
FatI	CATG	9 cut(s)	439, 503, 583, 712, 981, 1008, 1464, 1503, 1633
FauI	CCCGC	1 cut(s)	136
FbaI	TGATCA	1 cut(s)	1180
FokI	GGATG	4 cut(s)	107, 1019, 1106, 1609
FriOI	GRGCYC	1 cut(s)	1212
FspBI	CTAG	1 cut(s)	1481
GlaI	GCGC	1 cut(s)	1565
GsaI	CCCAGC	1 cut(s)	1322
HaeIII	GGCC	3 cut(s)	264, 1190, 1394
HapII	CCGG	4 cut(s)	870, 1373, 1391, 1625
HgaI	GACGC	1 cut(s)	1393
HhaI	GCGC	1 cut(s)	1566
Hin1II	CATG	9 cut(s)	443, 507, 587, 716, 985, 1012, 1468, 1507, 1637
Hin6I	GCGC	1 cut(s)	1564
HinP1I	GCGC	1 cut(s)	1564
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HindIII	AAGCTT	1 cut(s)	423
HinfI	GANTC	3 cut(s)	434, 472, 827
HpaI	GTTAAC	1 cut(s)	316
HpaII	CCGG	4 cut(s)	870, 1373, 1391, 1625
HphI	GGTGA	4 cut(s)	637, 664, 884, 1204
Hpy166II	GTNNAC	3 cut(s)	316, 763, 1517
Hpy188I	TCNGA	3 cut(s)	832, 946, 1137
Hpy188III	TCNNGA	8 cut(s)	191, 476, 530, 551, 898, 1009, 1276, 1664
Hpy8I	GTNNAC	3 cut(s)	316, 763, 1517
Hpy99I	CGWCG	2 cut(s)	953, 1583
HpyAV	CCTTC	5 cut(s)	361, 968, 1257, 1278, 1326
HpyCH4III	ACNGT	3 cut(s)	70, 1019, 1557
HpyCH4IV	ACGT	3 cut(s)	169, 186, 769
HpyF10VI	GCNNNNNNNGC	5 cut(s)	124, 753, 1207, 1373, 1616
HpyF3I	CTNAG	4 cut(s)	17, 511, 722, 1355
HpySE526I	ACGT	3 cut(s)	169, 186, 769
Hsp92II	CATG	9 cut(s)	443, 507, 587, 716, 985, 1012, 1468, 1507, 1637
HspAI	GCGC	1 cut(s)	1564
KflI	GGGWCCC	1 cut(s)	6
Ksp22I	TGATCA	1 cut(s)	1180
KspAI	GTTAAC	1 cut(s)	316
Kzo9I	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	4 cut(s)	135, 356, 402, 1627
Lsp1109I	GCAGC	8 cut(s)	114, 134, 479, 768, 1230, 1323, 1531, 1559
LweI	GCATC	6 cut(s)	184, 597, 649, 1041, 1445, 1597
MaeI	CTAG	1 cut(s)	1481
MaeII	ACGT	3 cut(s)	169, 186, 769
MaeIII	GTNAC	6 cut(s)	70, 105, 248, 703, 1288, 1551
MalI	GATC	7 cut(s)	140, 545, 834, 1182, 1221, 1352, 1662
MboI	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
MflI	RGATCY	2 cut(s)	543, 1350
MhlI	GDGCHC	1 cut(s)	1212
MlsI	TGGCCA	1 cut(s)	264
MluI	ACGCGT	1 cut(s)	1365
MluNI	TGGCCA	1 cut(s)	264
MlyI	GAGTC	2 cut(s)	443, 481
Mox20I	TGGCCA	1 cut(s)	264
MroXI	GAANNNNTTC	1 cut(s)	1655
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	4 cut(s)	135, 315, 498, 1064
MslI	CAYNNNNRTG	3 cut(s)	650, 870, 1227
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	4 cut(s)	127, 1243, 1322, 1544
MspCI	CTTAAG	1 cut(s)	134
MspI	CCGG	4 cut(s)	870, 1373, 1391, 1625
MspR9I	CCNGG	1 cut(s)	650
MvaI	CCWGG	1 cut(s)	650
MvnI	CGCG	3 cut(s)	143, 848, 1367
MwoI	GCNNNNNNNGC	5 cut(s)	124, 753, 1207, 1373, 1616
NcoI	CCATGG	1 cut(s)	1464
NdeII	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
NheI	GCTAGC	1 cut(s)	1480
NlaIII	CATG	9 cut(s)	443, 507, 587, 716, 985, 1012, 1468, 1507, 1637
NlaIV	GGNNCC	4 cut(s)	7, 8, 728, 896
NmeAIII	GCCGAG	1 cut(s)	1177
NmuCI	GTSAC	2 cut(s)	105, 248
NspI	RCATGY	1 cut(s)	985
NspV	TTCGAA	1 cut(s)	629
OliI	CACNNNNGTG	1 cut(s)	650
PagI	TCATGA	1 cut(s)	1008
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	1 cut(s)	1224
PdmI	GAANNNNTTC	1 cut(s)	1655
PfeI	GAWTC	1 cut(s)	827
PflMI	CCANNNNNTGG	1 cut(s)	570
PinAI	ACCGGT	1 cut(s)	869
PleI	GAGTC	2 cut(s)	442, 480
PpsI	GAGTC	2 cut(s)	442, 480
PpuMI	RGGWCCY	2 cut(s)	6, 727
Psp124BI	GAGCTC	1 cut(s)	1212
Psp1406I	AACGTT	1 cut(s)	186
Psp5II	RGGWCCY	2 cut(s)	6, 727
Psp6I	CCWGG	1 cut(s)	648
PspFI	CCCAGC	1 cut(s)	1318
PspGI	CCWGG	1 cut(s)	648
PspN4I	GGNNCC	4 cut(s)	7, 8, 728, 896
PspPI	GGNCC	3 cut(s)	6, 370, 727
PspPPI	RGGWCCY	2 cut(s)	6, 727
PstI	CTGCAG	2 cut(s)	1383, 1549
PstNI	CAGNNNCTG	1 cut(s)	1561
PsuI	RGATCY	2 cut(s)	543, 1350
PvuII	CAGCTG	2 cut(s)	1243, 1322
RsaI	GTAC	3 cut(s)	956, 1021, 1424
RsaNI	GTAC	3 cut(s)	955, 1020, 1423
RseI	CAYNNNNRTG	3 cut(s)	650, 870, 1227
SacI	GAGCTC	1 cut(s)	1212
SapI	GCTCTTC	1 cut(s)	741
SaqAI	TTAA	4 cut(s)	135, 315, 498, 1064
Sau3AI	GATC	7 cut(s)	138, 543, 832, 1180, 1219, 1350, 1660
Sau96I	GGNCC	3 cut(s)	6, 370, 727
SchI	GAGTC	2 cut(s)	443, 481
ScrFI	CCNGG	1 cut(s)	650
SduI	GDGCHC	1 cut(s)	1212
SfaNI	GCATC	6 cut(s)	184, 597, 649, 1041, 1445, 1597
SfcI	CTRYAG	3 cut(s)	1346, 1379, 1545
SfuI	TTCGAA	1 cut(s)	629
SinI	GGWCC	3 cut(s)	6, 370, 727
SmiMI	CAYNNNNRTG	3 cut(s)	650, 870, 1227
SmlI	CTYRAG	5 cut(s)	119, 134, 1072, 1274, 1282
SmoI	CTYRAG	5 cut(s)	119, 134, 1072, 1274, 1282
SsiI	CCGC	5 cut(s)	125, 143, 284, 328, 1542
SspMI	CTAG	1 cut(s)	1481
SstI	GAGCTC	1 cut(s)	1212
StyD4I	CCNGG	1 cut(s)	648
StyI	CCWWGG	1 cut(s)	1464
TaaI	ACNGT	3 cut(s)	70, 1019, 1557
TaiI	ACGT	3 cut(s)	172, 189, 772
TatI	WGTACW	2 cut(s)	1019, 1422
TauI	GCSGC	3 cut(s)	127, 287, 331
TfiI	GAWTC	1 cut(s)	827
Tru1I	TTAA	4 cut(s)	135, 315, 498, 1064
Tru9I	TTAA	4 cut(s)	135, 315, 498, 1064
TscAI	CASTG	4 cut(s)	114, 120, 754, 1383
TseFI	GTSAC	2 cut(s)	105, 248
TseI	GCWGC	8 cut(s)	127, 147, 467, 756, 1243, 1336, 1544, 1547
Tsp45I	GTSAC	2 cut(s)	105, 248
TspDTI	ATGAA	9 cut(s)	72, 351, 609, 728, 854, 930, 961, 1025, 1622
TspRI	CASTG	4 cut(s)	114, 120, 754, 1383
Van91I	CCANNNNNTGG	1 cut(s)	570
Vha464I	CTTAAG	1 cut(s)	134
VpaK11BI	GGWCC	3 cut(s)	6, 370, 727
XapI	RAATTY	5 cut(s)	12, 176, 295, 934, 1447
XceI	RCATGY	1 cut(s)	985
XmnI	GAANNNNTTC	1 cut(s)	1655
XspI	CTAG	1 cut(s)	1481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.