Basic Information

Type: Sequence Only

Biological Identity

Species

rosa_samantha

Chromosome

Unknown

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

0 .. 0

Gene Structure 1 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG405400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGGCAGTGGCAGACTTCTTCGCCGGAGAGATTATCACGGAGCTCCTGAAGAACCTGATAGCCATATCCAAAAAATCATGGCAGTTCAAAGACAGCGCCAACGGTCTCAAAGACTCCATAGAACAGCTCCTCCCCATAATCCAAGAAATCAAGTACTCCGGCGTCGAGTTGCCGGCCATCCGCCAGTTTCAGCTCGACCGTCTCTCGGAGACTCTCCGGCAGGGACATGAGCTGGCCCACAAGGTCCTCCTCTCCTCTCGCTACAACATTTACAAGAACCTCCAATATGCCCGCAAGATGGAGAGGATCGAAAAGACCGTCGCCAAGTTCATCCAGGGCCCATTGCAGGCCCATATCTTGGCCGACGTCCACCACATAAGGTTCCAGACCACCGAGCGCTTCGACCGCCTGGAAGGCTCCAGCTACCGCCTGGAGCAGAGGCTCGGCGCTATAAAGATTGGAGCTTGCAGTGGCGGCGGAGGTGTGTCGTGGGTGGAAGAGGCTGTGAGGCGCGTGGAGGAGGAGGGTCGGATGCAATGGGAGAGCAGCAGTGAGTTGGTTTTGGGCTTGGGTTTCGGGTTGAAGAAAGTCAAGGAAATGGTTCTTGGCAGAGATGACTTGTTCGTTGGGATTAGTGGGATTGGCGGCTCGGGAAAAACCACTCTTGTTAAGGAGTTGATTAAGGATGATCATGTTCGATCTTACTTTGGTGAGAGAATCCTGTTCCTGACGGTGTCACAATCTCCGGATATAGAGGATTTGAAGGCCAAGATCTTGGGGTTCATTATGGGGGGTCAAGTTTCGAGTCCCAGTTCTGTGCTTCCTCAAATGAACTATCAGTTTGAATGGAGAAACCCAACCCTGGTGGTTTTGGATGATGTTTGGTCACTTTCAACGCTTCAGCCACTGCTCTTCAACATTCCTGGTTGTAAAACCCTTGTGGTTTCACGTTTCAAATTCCAAACAGTAACAAAAGCCACCTATGACGTGGAGTTGCTGGGTGAAGATGAAGCAGTCTCTCTGTTTTGCCACTCTGCTTTTGGCCAAACGTTCATTCCTCCTGGTGCTGATAAGAAATTGGTCAAGCAGATTGTTGATGAGTGTAAGAGGCTTCCTTTGGCTCTCAAAGTGATTGGGTCTTCTTTACGAGACCAGCCTGAAATGATTTGGGCAAATGCAAGCAAAAGGTTGTCACGAGGGGAAAGCATCGGCGAGTCGCATGAGAACAACCTACTTGATCGGATGGCAATCAGTGTGAAATGCTTAAGCCCCAGAGTCAGGGAATGTTTCCTGGATTTGGGTGCCTTTCCAGAAGACAAGAAGATCCCACTGGATGTTCTTATCAACATTTGGGTTGAAACCCATGATCACGTTGATGAAGAACAAGCCTTTGCTATTCTTCACGAGCTTTCGGACAAGAATCTTGTCACCCTAGTGAAAGATGCAAGAGATGGAGACATATATAGCAGTTGCTATGACATCTCAGTTACACAACATGATGTCTTGCGAGACCTTGCTCTTCATTTGAGTAATAATGGCTCCGTGAATCAACGCAGCCGATTACTGATGCCAAGAAGAGAGATAGAACTTCCAAGAGAATGGGACAGGCATTCCGATGAACCATTTCAAGCTCGAATTGTATCGATTCATACAGGTGAAATGACAGAGATGGATTGGTTCGAGATGGAATTTCCCAAGGCTGAAGTGCTCATCTTGAATTTCGCCTCCAACGAGTACTTGTTACCTCCTTTTATTCGTAACATGCCAAAGTTGAAGGCATTGATCTTGATAAATTACAGTGCATCCAATGCAATTCTCCATGGTTTTTCTGTCTTGTCAAATTTGGGCAACTTGAGAAGCCTATGGCTTGAAAAAGTCTCAGTGCCTCCCTTAGACCCTGCTACCTTCCCTTCCAAAAAATTACGCAAGATTTCATTAATCTTGTGTAAGATTAATAAGAGCCTCGATCAATCAGCATTTCCTCGGCTCTTAGAACTCACAATTGATCATTGTGATGATTTGTTCGAGCTACCTTCAAGCATTTGTGGGATGCATTTGCTTAAAAATCTAAGCATAACCAACTGTCACCATCTATGGCAACTGCCAGCGGATTTGAGTAAGCTGAAATCTCTACAAATCCTAAGGCTATATGCATGCCCACATCTGCAAAAACTTCCTCCTGACATTTGTGAGCTCTGTTGCTTGAAGTATCTAAACATTTCCCAATGCTGTAGTTTGAGGGGCCTTCCCTTAAACATTGGTAAGTTGGCAAGTTTGGAAAAGATTGACATGAGGGAATGCTCACAAATCAGGAACCTTCCAAATTCTACAGCAGCTTTACCATCTCTTGGTCGTGTAGTATGTGATGAAGAGATTTCTTGGTTCTGGAAGAAGGCATTGCCAGACCTTCATGTTCAGGTTGCAGAGAAAAGTTTCGATCTTGACTGGCTTGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

819

Amino Acids

92.76

Weight (kDa)

6.23

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	9 - 129	3.9e-14	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	196 - 349	3.9e-15	NB-ARC domain
LRR_14	PF23598	640 - 768	1.6e-11	Leucine-rich repeat region
LRR_14	PF23598	728 - 791	1.4e-06	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	369
AccB1I	GGYRCC	1 cut(s)	1301
AccB7I	CCANNNNNTGG	2 cut(s)	358, 2348
AccII	CGCG	1 cut(s)	513
AccIII	TCCGGA	1 cut(s)	745
AciI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2108
AclI	AACGTT	1 cut(s)	1049
AclWI	GGATC	2 cut(s)	314, 1318
AcoI	YGGCCR	3 cut(s)	174, 360, 1042
AcsI	RAATTY	5 cut(s)	956, 1688, 1717, 1840, 2323
AcuI	CTGAAG	3 cut(s)	68, 884, 1722
AcyI	GRCGYC	2 cut(s)	162, 366
AfaI	GTAC	2 cut(s)	155, 1736
AfeI	AGCGCT	1 cut(s)	398
AfiI	CCNNNNNNNGG	8 cut(s)	205, 298, 346, 358, 862, 1278, 1297, 2348
AflII	CTTAAG	1 cut(s)	1264
AjiI	CACGTC	1 cut(s)	988
AjnI	CCWGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
AjuI	GAANNNNNNNTTGG	4 cut(s)	588, 620, 1038, 1070
AleI	CACNNNNGTG	1 cut(s)	1433
AloI	GAACNNNNNNTCC	2 cut(s)	114, 146
Alw21I	GWGCWC	3 cut(s)	45, 1710, 2196
Alw26I	GTCTC	8 cut(s)	110, 203, 206, 1021, 1143, 1449, 1503, 1882
AlwI	GGATC	2 cut(s)	314, 1318
AlwNI	CAGNNNCTG	1 cut(s)	907
Ama87I	CYCGRG	1 cut(s)	649
Aor13HI	TCCGGA	1 cut(s)	745
Aor51HI	AGCGCT	1 cut(s)	398
AoxI	GGCC	8 cut(s)	174, 234, 337, 348, 360, 765, 1042, 2242
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	3 cut(s)	546, 1554, 2333
ApoI	RAATTY	5 cut(s)	956, 1688, 1717, 1840, 2323
ArsI	GACNNNNNNTTYG	2 cut(s)	303, 335
AseI	ATTAAT	2 cut(s)	1937, 1953
Asp700I	GAANNNNTTC	3 cut(s)	600, 1623, 2432
AspLEI	GCGC	4 cut(s)	98, 399, 449, 513
AspS9I	GGNCC	6 cut(s)	235, 244, 337, 338, 349, 2242
AsuHPI	GGTGA	5 cut(s)	722, 1013, 1420, 1667, 2078
AvaI	CYCGRG	1 cut(s)	649
AvaII	GGWCC	1 cut(s)	244
AxyI	CCTNAGG	1 cut(s)	2141
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	1 cut(s)	1044
BanI	GGYRCC	1 cut(s)	1301
BanII	GRGCYC	3 cut(s)	45, 341, 2196
BauI	CACGAG	2 cut(s)	1194, 1403
BbsI	GAAGAC	2 cut(s)	1131, 1320
Bbv12I	GWGCWC	3 cut(s)	45, 1710, 2196
BbvI	GCAGC	3 cut(s)	558, 1566, 2345
BccI	CCATC	8 cut(s)	185, 292, 1237, 1445, 1663, 1678, 2097, 2350
BciT130I	CCWGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
BclI	TGATCA	3 cut(s)	688, 1366, 2005
BcoDI	GTCTC	8 cut(s)	110, 203, 206, 1021, 1143, 1449, 1503, 1882
BfaI	CTAG	1 cut(s)	1433
BfmI	CTRYAG	2 cut(s)	2230, 2328
BfoI	RGCGCY	3 cut(s)	99, 400, 450
BfrI	CTTAAG	1 cut(s)	1264
BglI	GCCNNNNNGGC	1 cut(s)	414
BglII	AGATCT	1 cut(s)	771
BisI	GCNGC	5 cut(s)	475, 547, 646, 1555, 2334
BlsI	GCNGC	5 cut(s)	476, 548, 647, 1556, 2335
BmcAI	AGTACT	2 cut(s)	155, 1736
Bme1390I	CCNGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
Bme18I	GGWCC	1 cut(s)	244
BmeT110I	CYCGRG	1 cut(s)	649
BmgBI	CACGTC	1 cut(s)	988
BmgT120I	GGNCC	6 cut(s)	235, 244, 337, 338, 349, 2242
BmiI	GGNNCC	7 cut(s)	339, 383, 418, 1303, 1540, 2243, 2315
BmrFI	CCNGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
BmrI	ACTGGG	1 cut(s)	804
BmsI	GCATC	6 cut(s)	522, 1215, 1432, 1557, 1811, 2040
BmuI	ACTGGG	1 cut(s)	804
BpiI	GAAGAC	2 cut(s)	1131, 1320
BpmI	CTGGAG	2 cut(s)	403, 452
BpuEI	CTTGAG	1 cut(s)	1873
Bsa29I	ATCGAT	1 cut(s)	1643
BsaBI	GATNNNNATC	1 cut(s)	1247
BsaHI	GRCGYC	2 cut(s)	162, 366
BsaI	GGTCTC	3 cut(s)	110, 1143, 1503
BsaJI	CCNNGG	5 cut(s)	334, 861, 1695, 1819, 1982
BsaWI	WCCGGW	1 cut(s)	745
BsaXI	ACNNNNNCTCC	2 cut(s)	114, 144
Bsc4I	CCNNNNNNNGG	8 cut(s)	205, 298, 346, 358, 862, 1278, 1297, 2348
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	4 cut(s)	184, 810, 1335, 2450
Bse21I	CCTNAGG	1 cut(s)	2141
Bse3DI	GCAATG	3 cut(s)	341, 542, 2396
Bse8I	GATNNNNATC	1 cut(s)	1247
BseAI	TCCGGA	1 cut(s)	745
BseBI	CCWGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
BseCI	ATCGAT	1 cut(s)	1643
BseDI	CCNNGG	5 cut(s)	334, 861, 1695, 1819, 1982
BseGI	GGATG	9 cut(s)	177, 330, 537, 691, 880, 1248, 1339, 1802, 2055
BseJI	GATNNNNATC	1 cut(s)	1247
BseLI	CCNNNNNNNGG	8 cut(s)	205, 298, 346, 358, 862, 1278, 1297, 2348
BseMI	GCAATG	3 cut(s)	341, 542, 2396
BseMII	CTCAG	2 cut(s)	1497, 1893
BseNI	ACTGG	4 cut(s)	184, 810, 1335, 2450
BseRI	GAGGAG	5 cut(s)	119, 239, 244, 533, 536
BseSI	GKGCMC	1 cut(s)	341
BseXI	GCAGC	3 cut(s)	558, 1566, 2345
BseYI	CCCAGC	1 cut(s)	997
Bsh1236I	CGCG	1 cut(s)	513
Bsh1285I	CGRYCG	2 cut(s)	199, 406
BshFI	GGCC	8 cut(s)	176, 236, 339, 350, 362, 767, 1044, 2244
BshNI	GGYRCC	1 cut(s)	1301
BshVI	ATCGAT	1 cut(s)	1643
BsiEI	CGRYCG	2 cut(s)	199, 406
BsiHKAI	GWGCWC	3 cut(s)	45, 1710, 2196
BsiHKCI	CYCGRG	1 cut(s)	649
BsiSI	CCGG	5 cut(s)	24, 159, 173, 217, 746
BslFI	GGGAC	3 cut(s)	237, 792, 1616
BslI	CCNNNNNNNGG	8 cut(s)	205, 298, 346, 358, 862, 1278, 1297, 2348
BsmAI	GTCTC	8 cut(s)	110, 203, 206, 1021, 1143, 1449, 1503, 1882
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	3 cut(s)	237, 792, 1616
BsmI	GAATGC	2 cut(s)	1609, 2303
BsnI	GGCC	8 cut(s)	176, 236, 339, 350, 362, 767, 1044, 2244
Bso31I	GGTCTC	3 cut(s)	110, 1143, 1503
BsoBI	CYCGRG	1 cut(s)	649
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	4 cut(s)	45, 341, 1710, 2196
Bsp13I	TCCGGA	1 cut(s)	745
Bsp19I	CCATGG	1 cut(s)	1819
BspACI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2108
BspANI	GGCC	8 cut(s)	176, 236, 339, 350, 362, 767, 1044, 2244
BspCNI	CTCAG	2 cut(s)	1496, 1892
BspDI	ATCGAT	1 cut(s)	1643
BspEI	TCCGGA	1 cut(s)	745
BspFNI	CGCG	1 cut(s)	513
BspLI	GGNNCC	7 cut(s)	339, 383, 418, 1303, 1540, 2243, 2315
BspPI	GGATC	2 cut(s)	314, 1318
BspQI	GCTCTTC	2 cut(s)	917, 1524
BspT107I	GGYRCC	1 cut(s)	1301
BspTI	CTTAAG	1 cut(s)	1264
BspTNI	GGTCTC	3 cut(s)	110, 1143, 1503
BsrDI	GCAATG	3 cut(s)	341, 542, 2396
BsrFI	RCCGGY	1 cut(s)	172
BsrI	ACTGG	4 cut(s)	184, 810, 1335, 2450
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	5 cut(s)	334, 861, 1695, 1819, 1982
BssNI	GRCGYC	2 cut(s)	162, 366
BssSI	CACGAG	2 cut(s)	1194, 1403
BssT1I	CCWWGG	2 cut(s)	1695, 1819
Bst2BI	CACGAG	2 cut(s)	1194, 1403
Bst2UI	CCWGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
Bst4CI	ACNGT	7 cut(s)	104, 200, 319, 733, 967, 1799, 2084
Bst6I	CTCTTC	5 cut(s)	492, 917, 1524, 1570, 2364
BstACI	GRCGYC	2 cut(s)	162, 366
BstAFI	CTTAAG	1 cut(s)	1264
BstAPI	GCANNNNNTGC	1 cut(s)	1808
BstC8I	GCNNGC	8 cut(s)	174, 234, 292, 348, 466, 1180, 2106, 2155
BstDEI	CTNAG	6 cut(s)	1483, 1879, 1891, 1990, 2069, 2141
BstDSI	CCRYGG	1 cut(s)	1819
BstF5I	GGATG	9 cut(s)	177, 330, 537, 691, 880, 1248, 1339, 1802, 2055
BstFNI	CGCG	1 cut(s)	513
BstH2I	RGCGCY	3 cut(s)	99, 400, 450
BstHHI	GCGC	4 cut(s)	98, 399, 449, 513
BstMAI	GTCTC	8 cut(s)	110, 203, 206, 1021, 1143, 1449, 1503, 1882
BstMCI	CGRYCG	2 cut(s)	199, 406
BstMWI	GCNNNNNNNGC	4 cut(s)	405, 414, 474, 1808
BstNI	CCWGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
BstNSI	RCATGY	2 cut(s)	1765, 2157
BstSCI	CCNGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
BstSFI	CTRYAG	2 cut(s)	2230, 2328
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	1 cut(s)	513
BstV1I	GCAGC	3 cut(s)	558, 1566, 2345
BstV2I	GAAGAC	2 cut(s)	1131, 1320
BstX2I	RGATCY	2 cut(s)	771, 1323
BstXI	CCANNNNNNTGG	2 cut(s)	775, 1599
BstYI	RGATCY	2 cut(s)	771, 1323
Bsu15I	ATCGAT	1 cut(s)	1643
Bsu36I	CCTNAGG	1 cut(s)	2141
BsuRI	GGCC	8 cut(s)	176, 236, 339, 350, 362, 767, 1044, 2244
BsuTUI	ATCGAT	1 cut(s)	1643
BtgI	CCRYGG	1 cut(s)	1819
BtrI	CACGTC	1 cut(s)	988
BtsCI	GGATG	9 cut(s)	177, 330, 537, 691, 880, 1248, 1339, 1802, 2055
BtsI	GCAGTG	4 cut(s)	12, 475, 556, 905
BtsIMutI	CAGTG	8 cut(s)	12, 475, 556, 905, 1258, 1328, 1804, 1887
Cac8I	GCNNGC	8 cut(s)	174, 234, 292, 348, 466, 1180, 2106, 2155
CaiI	CAGNNNCTG	1 cut(s)	907
CfoI	GCGC	4 cut(s)	98, 399, 449, 513
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	6 cut(s)	235, 244, 337, 338, 349, 2242
ClaI	ATCGAT	1 cut(s)	1643
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	2 cut(s)	154, 1735
CspCI	CAANNNNNGTGG	2 cut(s)	846, 881
CviQI	GTAC	2 cut(s)	154, 1735
DdeI	CTNAG	6 cut(s)	1483, 1879, 1891, 1990, 2069, 2141
EaeI	YGGCCR	3 cut(s)	174, 360, 1042
Eam1104I	CTCTTC	5 cut(s)	492, 917, 1524, 1570, 2364
EarI	CTCTTC	5 cut(s)	492, 917, 1524, 1570, 2364
EciI	GGCGGA	2 cut(s)	170, 492
Ecl136II	GAGCTC	2 cut(s)	43, 2194
Eco130I	CCWWGG	2 cut(s)	1695, 1819
Eco24I	GRGCYC	3 cut(s)	45, 341, 2196
Eco31I	GGTCTC	3 cut(s)	110, 1143, 1503
Eco47I	GGWCC	1 cut(s)	244
Eco47III	AGCGCT	1 cut(s)	398
Eco53kI	GAGCTC	2 cut(s)	43, 2194
Eco57I	CTGAAG	3 cut(s)	68, 884, 1722
Eco81I	CCTNAGG	1 cut(s)	2141
Eco88I	CYCGRG	1 cut(s)	649
EcoICRI	GAGCTC	2 cut(s)	43, 2194
EcoO109I	RGGNCCY	3 cut(s)	244, 337, 2242
EcoRII	CCWGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
EcoT14I	CCWWGG	2 cut(s)	1695, 1819
EcoT22I	ATGCAT	2 cut(s)	2055, 2155
EcoT38I	GRGCYC	3 cut(s)	45, 341, 2196
ErhI	CCWWGG	2 cut(s)	1695, 1819
Esp3I	CGTCTC	1 cut(s)	206
FaqI	GGGAC	3 cut(s)	237, 792, 1616
FauI	CCCGC	1 cut(s)	299
FbaI	TGATCA	3 cut(s)	688, 1366, 2005
Fnu4HI	GCNGC	5 cut(s)	475, 547, 646, 1555, 2334
FokI	GGATG	9 cut(s)	164, 317, 544, 698, 887, 1255, 1346, 1789, 2062
FriOI	GRGCYC	3 cut(s)	45, 341, 2196
Fsp4HI	GCNGC	5 cut(s)	475, 547, 646, 1555, 2334
FspBI	CTAG	1 cut(s)	1433
GlaI	GCGC	4 cut(s)	97, 398, 448, 512
GluI	GCNGC	5 cut(s)	475, 547, 646, 1555, 2334
GsaI	CCCAGC	1 cut(s)	1001
GsuI	CTGGAG	2 cut(s)	403, 452
HaeII	RGCGCY	3 cut(s)	99, 400, 450
HaeIII	GGCC	8 cut(s)	176, 236, 339, 350, 362, 767, 1044, 2244
HapII	CCGG	5 cut(s)	24, 159, 173, 217, 746
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	4 cut(s)	98, 399, 449, 513
Hin1I	GRCGYC	2 cut(s)	162, 366
Hin6I	GCGC	4 cut(s)	96, 397, 447, 511
HinP1I	GCGC	4 cut(s)	96, 397, 447, 511
HinfI	GANTC	9 cut(s)	113, 211, 717, 805, 1214, 1275, 1420, 1546, 1645
HpaII	CCGG	5 cut(s)	24, 159, 173, 217, 746
HphI	GGTGA	5 cut(s)	722, 1013, 1420, 1667, 2078
Hpy166II	GTNNAC	1 cut(s)	370
Hpy188I	TCNGA	5 cut(s)	208, 531, 1242, 1414, 1615
Hpy8I	GTNNAC	1 cut(s)	370
Hpy99I	CGWCG	3 cut(s)	167, 323, 368
HpyCH4III	ACNGT	7 cut(s)	104, 200, 319, 733, 967, 1799, 2084
HpyCH4IV	ACGT	5 cut(s)	366, 949, 987, 1049, 1371
HpyF10VI	GCNNNNNNNGC	4 cut(s)	405, 414, 474, 1808
HpyF3I	CTNAG	6 cut(s)	1483, 1879, 1891, 1990, 2069, 2141
HpySE526I	ACGT	5 cut(s)	366, 949, 987, 1049, 1371
Hsp92I	GRCGYC	2 cut(s)	162, 366
HspAI	GCGC	4 cut(s)	96, 397, 447, 511
Kpn2I	TCCGGA	1 cut(s)	745
KroI	GCCGGC	1 cut(s)	172
KroNI	GCCGGC	1 cut(s)	174
Ksp22I	TGATCA	3 cut(s)	688, 1366, 2005
LguI	GCTCTTC	2 cut(s)	917, 1524
LmnI	GCTCC	7 cut(s)	40, 48, 132, 422, 433, 461, 1544
Lsp1109I	GCAGC	3 cut(s)	558, 1566, 2345
LweI	GCATC	6 cut(s)	522, 1215, 1432, 1557, 1811, 2040
MaeI	CTAG	1 cut(s)	1433
MaeII	ACGT	5 cut(s)	366, 949, 987, 1049, 1371
MaeIII	GTNAC	9 cut(s)	735, 885, 967, 1191, 1426, 1486, 1740, 1757, 2084
MfeI	CAATTG	1 cut(s)	2001
MflI	RGATCY	2 cut(s)	771, 1323
MhlI	GDGCHC	4 cut(s)	45, 341, 1710, 2196
MlsI	TGGCCA	1 cut(s)	1044
MluNI	TGGCCA	1 cut(s)	1044
MlyI	GAGTC	5 cut(s)	107, 205, 814, 1223, 1284
MmeI	TCCRAC	2 cut(s)	509, 1752
Mox20I	TGGCCA	1 cut(s)	1044
Mph1103I	ATGCAT	2 cut(s)	2055, 2155
MroI	TCCGGA	1 cut(s)	745
MroNI	GCCGGC	1 cut(s)	172
MroXI	GAANNNNTTC	3 cut(s)	600, 1623, 2432
MscI	TGGCCA	1 cut(s)	1044
MseI	TTAA	7 cut(s)	669, 681, 1265, 1937, 1953, 2061, 2252
MslI	CAYNNNNRTG	5 cut(s)	1374, 1433, 1614, 1653, 2013
Msp20I	TGGCCA	1 cut(s)	1044
MspA1I	CMGCKG	1 cut(s)	2108
MspCI	CTTAAG	1 cut(s)	1264
MspI	CCGG	5 cut(s)	24, 159, 173, 217, 746
MspR9I	CCNGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
MunI	CAATTG	1 cut(s)	2001
Mva1269I	GAATGC	2 cut(s)	1609, 2303
MvaI	CCWGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
MvnI	CGCG	1 cut(s)	513
MwoI	GCNNNNNNNGC	4 cut(s)	405, 414, 474, 1808
NaeI	GCCGGC	1 cut(s)	174
NcoI	CCATGG	1 cut(s)	1819
NgoMIV	GCCGGC	1 cut(s)	172
NlaIV	GGNNCC	7 cut(s)	339, 383, 418, 1303, 1540, 2243, 2315
NmeAIII	GCCGAG	2 cut(s)	423, 1963
NmuCI	GTSAC	5 cut(s)	735, 885, 1191, 1426, 2084
NsiI	ATGCAT	2 cut(s)	2055, 2155
NspI	RCATGY	2 cut(s)	1765, 2157
OliI	CACNNNNGTG	1 cut(s)	1433
PaeI	GCATGC	1 cut(s)	2157
PciSI	GCTCTTC	2 cut(s)	917, 1524
PcsI	WCGNNNNNNNCGW	2 cut(s)	315, 1728
PctI	GAATGC	2 cut(s)	1609, 2303
PdiI	GCCGGC	1 cut(s)	174
PdmI	GAANNNNTTC	3 cut(s)	600, 1623, 2432
PfeI	GAWTC	4 cut(s)	717, 1420, 1546, 1645
PflFI	GACNNNGTC	1 cut(s)	733
PflMI	CCANNNNNTGG	2 cut(s)	358, 2348
PfoI	TCCNGGA	1 cut(s)	1290
PkrI	GCNGC	5 cut(s)	476, 548, 647, 1556, 2335
PleI	GAGTC	5 cut(s)	107, 205, 813, 1222, 1283
PpsI	GAGTC	5 cut(s)	107, 205, 813, 1222, 1283
PpuMI	RGGWCCY	1 cut(s)	244
PshBI	ATTAAT	2 cut(s)	1937, 1953
Psp124BI	GAGCTC	2 cut(s)	45, 2196
Psp1406I	AACGTT	1 cut(s)	1049
Psp5II	RGGWCCY	1 cut(s)	244
Psp6I	CCWGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
PspFI	CCCAGC	1 cut(s)	997
PspGI	CCWGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
PspN4I	GGNNCC	7 cut(s)	339, 383, 418, 1303, 1540, 2243, 2315
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	6 cut(s)	235, 244, 337, 338, 349, 2242
PspPPI	RGGWCCY	1 cut(s)	244
PstNI	CAGNNNCTG	1 cut(s)	907
PsuI	RGATCY	2 cut(s)	771, 1323
PsyI	GACNNNGTC	1 cut(s)	733
RsaI	GTAC	2 cut(s)	155, 1736
RsaNI	GTAC	2 cut(s)	154, 1735
RseI	CAYNNNNRTG	5 cut(s)	1374, 1433, 1614, 1653, 2013
SacI	GAGCTC	2 cut(s)	45, 2196
SapI	GCTCTTC	2 cut(s)	917, 1524
SaqAI	TTAA	7 cut(s)	669, 681, 1265, 1937, 1953, 2061, 2252
SatI	GCNGC	5 cut(s)	475, 547, 646, 1555, 2334
Sau96I	GGNCC	6 cut(s)	235, 244, 337, 338, 349, 2242
ScaI	AGTACT	2 cut(s)	155, 1736
SchI	GAGTC	5 cut(s)	107, 205, 814, 1223, 1284
ScrFI	CCNGG	7 cut(s)	335, 410, 431, 863, 924, 1062, 1292
SduI	GDGCHC	4 cut(s)	45, 341, 1710, 2196
SfaNI	GCATC	6 cut(s)	522, 1215, 1432, 1557, 1811, 2040
SfcI	CTRYAG	2 cut(s)	2230, 2328
SinI	GGWCC	1 cut(s)	244
SmiMI	CAYNNNNRTG	5 cut(s)	1374, 1433, 1614, 1653, 2013
SmlI	CTYRAG	2 cut(s)	1264, 1852
SmoI	CTYRAG	2 cut(s)	1264, 1852
SphI	GCATGC	1 cut(s)	2157
SsiI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2108
SspMI	CTAG	1 cut(s)	1433
SstI	GAGCTC	2 cut(s)	45, 2196
StyD4I	CCNGG	7 cut(s)	333, 408, 429, 861, 922, 1060, 1290
StyI	CCWWGG	2 cut(s)	1695, 1819
TaaI	ACNGT	7 cut(s)	104, 200, 319, 733, 967, 1799, 2084
TaiI	ACGT	5 cut(s)	369, 952, 990, 1052, 1374
TatI	WGTACW	2 cut(s)	153, 1734
TauI	GCSGC	2 cut(s)	477, 648
TfiI	GAWTC	4 cut(s)	717, 1420, 1546, 1645
Tru1I	TTAA	7 cut(s)	669, 681, 1265, 1937, 1953, 2061, 2252
Tru9I	TTAA	7 cut(s)	669, 681, 1265, 1937, 1953, 2061, 2252
TscAI	CASTG	8 cut(s)	12, 475, 556, 912, 1258, 1335, 1804, 1887
TseFI	GTSAC	5 cut(s)	735, 885, 1191, 1426, 2084
TseI	GCWGC	3 cut(s)	546, 1554, 2333
Tsp45I	GTSAC	5 cut(s)	735, 885, 1191, 1426, 2084
TspGWI	ACGGA	2 cut(s)	53, 1531
TspRI	CASTG	8 cut(s)	12, 475, 556, 912, 1258, 1335, 1804, 1887
Tth111I	GACNNNGTC	1 cut(s)	733
Van91I	CCANNNNNTGG	2 cut(s)	358, 2348
Vha464I	CTTAAG	1 cut(s)	1264
VpaK11BI	GGWCC	1 cut(s)	244
VspI	ATTAAT	2 cut(s)	1937, 1953
XapI	RAATTY	5 cut(s)	956, 1688, 1717, 1840, 2323
XceI	RCATGY	2 cut(s)	1765, 2157
XcmI	CCANNNNNNNNNTGG	3 cut(s)	427, 985, 1037
XmnI	GAANNNNTTC	3 cut(s)	600, 1623, 2432
XspI	CTAG	1 cut(s)	1433
ZraI	GACGTC	1 cut(s)	367
ZrmI	AGTACT	2 cut(s)	155, 1736
Zsp2I	ATGCAT	2 cut(s)	2055, 2155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.