Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

44483911 .. 44492023

Gene Structure 8113 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0293000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGGAGACAGTGAGACAGTCGTTGCTCAAACAAATGCAATGATGGGATATCCATCAGCAGCCTATACTACGACAGGTTATTCTGATTCCAGCTCAAATATTGCTCAGGAGGCTACCGCAGAAATTTCTGCTCCTCCTGCTCCTGTAGATGCAACCCATCCAATGCCTGGTGATGGAAATGCATATTCTGTGGCTTCCGATTCTCTCATGCAAGAATCACATTCTTATGCAACATATGAGGCTCAACCAGCTGTTGGTGTTGCTGATGCTGGTGGGAATGTTGCTAGCAGTGAACATGAAACCATGGCATCATTGCAAGCTGCCAGTTATGATTCTACTGCTAATGGTGTCGCTACTTCTGGGGTGGGAACTGTTTCATCTGTTGGAAATGGAGATGCTTCAGACAACATGGCTGGATCTCTGGATACACAACAATATGTGGATGGTTCTGCTATGTCTGCCGAAGAAGATAGACTATGGAACATTGTGAGGGCTAATTCTTTAGATTTTAATGCCTGGACTGCTTTAATTGAAGAGACAGAGAAGGTTGCAGAGGATAACATATTAAAAATTCGAAAAGTTTATGATGCTTTTCTAGCAGAGTTTCCTTTGTGTTATGGGTATTGGAAAAAGTATGCAGATCATGAGGCGCGTTTTGGTGTTATTGATAAAGTTGTGGAGGTTTATGAACGAGCAGTTCAAGCTGTTACCTATTCAGTTGACATTTGGTTGCATTACTGCACATTTGCCACAACTACTTTTGGAGATCCAGACACTATCAGAAGGCTTTTTGAGCGAGGATTAGCATATGTTGGAACAGATTACTTGTCCTTTCCTCTTTGGGACAGGTACATTGATTATGAATTTATGCAGCAAGCATGGGGCCAGCTTGTTGTGATCTACTCACGAATATTAGAGAATCCGAACCAACAACTGGATCGATATTTAAACAGTTTTAAGGAGTTAGCTGGAAGTCGGCCTCTCTCAGAATTGAGAACTGCTGAAGAAGCTGCTGCTGCTGCTGCAGCAGCAGCAAGTGCCACTTCAGAGGATGGTGTACAAGCAAACGGGGAAGATGCTCAACCTGATGGTGCAGAACCATCTCCTGCTATCTTAAAAGAAGCGGAGGAGTTGGAGAAGTTTATCGCCATTCGAGAAGAGATGTATAAAAAAGCTAAAGAGTTCGATTCTAAGATAATTAATTTTGAAACAGCTATTAGGAGGCCTTACTTTCACGTGAGGCCCCTTAACGCTGCAGAGCTTGAAAATTGGAACAACTATCTTGACTTTATTGAAAGGGAAGGTGACTTTAACAAGGTGGTCAAGTTATATGAAAGATGCCTGATTGCTTGCGCTAATTATCCTGAATACTGGATTCGTTATGTCCTAAGTATGGAAGCTAGTGGAAGTTTGGATCTTGCCAATAATGCTCTTGATCGTGCAACCCAGGTCTTTGTGAAGAGACAACCAGAGATTCACCTTTTTGCTGCTCGCTTCAAAGAGCAAAGTGGAGATATCCTTGGTGCTCAGGCTGCCTATCAACTCGTACATTCTGAAATTTCACCTGGTCTTCTTGAAGCAATCATTAAGCATGCAAACATGGAACACCGTCTGGGAAATATGGAAGCTGCCTACTCCTTGTTTGAACAGGCCATTGCCATTGAAAAAGGAAAGGAGCATTCTCAGACCTTACCCGTGCTGTTTGCTCAATACGCACGGTTCGCATACTTGGTATCTTTGAATGCTGAAAAAGCCAGGGCTATCTTTCTTGAAGCGGTCGAGCATGTTCAATTGTCAAAAGCATTCCTCGAGGCCCTGATACATTTCGAGTCGTTACATTCGTTGCCAAAGCAAATTGATTACATAGATTCCTTGGTTGTGAAGTTTGTAGAGTCCAACTCAGAGAACCCCAATTTTGCGAGTGTTGCTGACAGAGAGGAGTTATCTTGCATCTATTTGGAGTTTCTAAATCTCTTTGGGGATATACAGTCTATCAAGAAGGCTGAGGATCGGCATGCTAAGCTCTTTTTACTTCATAGGAGCACCACTTCAGAATTGAAAAAACGGCAAGCTGAAGATTACCTATCTTCTGATAAAGCAAAGATGGCAAAATCCTACTCTGGTGTTCCTTCACCTGCCCAGTCTATTATGGGAGCATATCCAAATGCACAAAACCAGTGGGCAGCTGGTTATGGTGTGCAACCTCAAGCTTGGCCACCAGCTACACAAGCACAGGGACAACAATGGACCCCTGGTTATACCCAACAGGCATACAATGCATATGGTGGTTATCAGCAAGTACCTGCTGCAGTGCCACAGACTGCTACATATCCCGCCACATATCCCGCCACATATCCTGCTCAGCCGGCGGCTTTCCCCCCACAAAGCTATGCACAGCCAGCAGCCGCAGCTGCTGCAGTAGCAGCTGTAGCACCAGCTCAGCAACCTGCTCCGGCTCAACAGGCGTATTATGGGAATTACTACCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

829

Amino Acids

91.34

Weight (kDa)

4.78

Isoelectric Point (pI)

43.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_PRP39_N	PF23240	157 - 321	9.8e-61	PRP39 N-terminal HAT repeat
Suf	PF05843	158 - 288	1.5e-13	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_N	PF23233	165 - 306	1e-07	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C	PF23241	209 - 270	3.3e-06	PRP39 C-terminal HAT repeat
Suf	PF05843	379 - 599	1.3e-06	Suppressor of forked protein (Suf)
HAT_PRP39_C	PF23241	398 - 537	7.2e-48	PRP39 C-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2143
AbsI	CCTCGAGG	1 cut(s)	1808
Acc36I	ACCTGC	3 cut(s)	2143, 2311, 2455
AccB7I	CCANNNNNTGG	3 cut(s)	167, 254, 934
AccII	CGCG	1 cut(s)	652
AciI	CCGC	7 cut(s)	117, 1124, 1775, 2334, 2346, 2369, 2406
AclWI	GGATC	5 cut(s)	424, 761, 945, 1422, 2016
AcoI	YGGCCR	1 cut(s)	2213
AcsI	RAATTY	4 cut(s)	123, 570, 863, 1557
AcuI	CTGAAG	5 cut(s)	384, 1023, 1029, 2034, 2094
AcvI	CACGTG	1 cut(s)	1237
AfaI	GTAC	4 cut(s)	851, 1059, 1548, 2301
AfiI	CCNNNNNNNGG	5 cut(s)	167, 173, 254, 934, 1393
AjnI	CCWGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
Alw21I	GWGCWC	2 cut(s)	1528, 2045
Alw26I	GTCTC	3 cut(s)	8, 530, 1456
AlwI	GGATC	5 cut(s)	424, 761, 945, 1422, 2016
AlwNI	CAGNNNCTG	1 cut(s)	2414
Ama87I	CYCGRG	1 cut(s)	1808
AoxI	GGCC	7 cut(s)	883, 977, 1223, 1241, 1650, 1812, 2213
ApoI	RAATTY	4 cut(s)	123, 570, 863, 1557
AseI	ATTAAT	1 cut(s)	1200
Asp700I	GAANNNNTTC	1 cut(s)	373
AspLEI	GCGC	2 cut(s)	652, 1355
AspS9I	GGNCC	4 cut(s)	883, 1242, 1813, 2247
AsuHPI	GGTGA	5 cut(s)	182, 1316, 1469, 1554, 2124
AsuII	TTCGAA	1 cut(s)	574
AsuNHI	GCTAGC	1 cut(s)	284
AvaI	CYCGRG	1 cut(s)	1808
AvaII	GGWCC	1 cut(s)	2247
BalI	TGGCCA	1 cut(s)	2215
BbrPI	CACGTG	1 cut(s)	1237
BbsI	GAAGAC	1 cut(s)	1562
Bbv12I	GWGCWC	2 cut(s)	1528, 2045
BbvCI	CCTCAGC	1 cut(s)	2004
BccI	CCATC	9 cut(s)	37, 61, 165, 167, 437, 1046, 1082, 1108, 2098
BceAI	ACGGC	1 cut(s)	2081
BciT130I	CCWGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
BciVI	GTATCC	1 cut(s)	418
BcoDI	GTCTC	3 cut(s)	8, 530, 1456
BfaI	CTAG	3 cut(s)	285, 596, 1401
BfmI	CTRYAG	6 cut(s)	144, 1023, 1254, 2307, 2415, 2427
BfuAI	ACCTGC	3 cut(s)	2143, 2311, 2455
BfuI	GTATCC	1 cut(s)	418
BlpI	GCTNAGC	3 cut(s)	2019, 2361, 2439
Bme1390I	CCNGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
Bme18I	GGWCC	1 cut(s)	2247
BmeT110I	CYCGRG	1 cut(s)	1808
BmgT120I	GGNCC	4 cut(s)	883, 1242, 1813, 2247
BmiI	GGNNCC	3 cut(s)	884, 1244, 2249
BmrFI	CCNGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
BmrI	ACTGGG	1 cut(s)	2134
BmsI	GCATC	8 cut(s)	139, 256, 317, 385, 577, 1066, 1328, 1959
BmtI	GCTAGC	1 cut(s)	288
BmuI	ACTGGG	1 cut(s)	2134
BpiI	GAAGAC	1 cut(s)	1562
BplI	GAGNNNNNCTC	2 cut(s)	1883, 1915
Bpu10I	CCTNAGC	3 cut(s)	105, 1527, 2004
Bpu1102I	GCTNAGC	3 cut(s)	2019, 2361, 2439
Bpu14I	TTCGAA	1 cut(s)	574
BpuEI	CTTGAG	1 cut(s)	2190
Bsa29I	ATCGAT	1 cut(s)	940
BsaAI	YACGTR	1 cut(s)	1237
BsaBI	GATNNNNATC	1 cut(s)	52
BsaJI	CCNNGG	6 cut(s)	303, 1446, 1519, 1755, 1872, 2251
Bsc4I	CCNNNNNNNGG	5 cut(s)	167, 173, 254, 934, 1393
Bse118I	RCCGGY	1 cut(s)	2365
Bse1I	ACTGG	6 cut(s)	324, 939, 1376, 2140, 2176, 2485
Bse3DI	GCAATG	3 cut(s)	45, 311, 1653
Bse8I	GATNNNNATC	1 cut(s)	52
BseBI	CCWGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
BseCI	ATCGAT	1 cut(s)	940
BseDI	CCNNGG	6 cut(s)	303, 1446, 1519, 1755, 1872, 2251
BseGI	GGATG	3 cut(s)	157, 448, 1057
BseJI	GATNNNNATC	1 cut(s)	52
BseLI	CCNNNNNNNGG	5 cut(s)	167, 173, 254, 934, 1393
BseMI	GCAATG	3 cut(s)	45, 311, 1653
BseMII	CTCAG	8 cut(s)	119, 999, 1541, 1697, 1914, 1995, 2375, 2453
BseNI	ACTGG	6 cut(s)	324, 939, 1376, 2140, 2176, 2485
BseRI	GAGGAG	3 cut(s)	123, 1142, 1952
BsgI	GTGCAG	2 cut(s)	724, 1113
Bsh1236I	CGCG	1 cut(s)	652
Bsh1285I	CGRYCG	1 cut(s)	1779
BshFI	GGCC	7 cut(s)	885, 979, 1225, 1243, 1652, 1814, 2215
BshVI	ATCGAT	1 cut(s)	940
BsiEI	CGRYCG	1 cut(s)	1779
BsiHKAI	GWGCWC	2 cut(s)	1528, 2045
BsiHKCI	CYCGRG	1 cut(s)	1808
BsiSI	CCGG	2 cut(s)	2366, 2453
BslFI	GGGAC	2 cut(s)	857, 2250
BslI	CCNNNNNNNGG	5 cut(s)	167, 173, 254, 934, 1393
BsmAI	GTCTC	3 cut(s)	8, 530, 1456
BsmFI	GGGAC	2 cut(s)	857, 2250
BsmI	GAATGC	3 cut(s)	1678, 1747, 1802
BsnI	GGCC	7 cut(s)	885, 979, 1225, 1243, 1652, 1814, 2215
BsoBI	CYCGRG	1 cut(s)	1808
Bsp119I	TTCGAA	1 cut(s)	574
Bsp1286I	GDGCHC	2 cut(s)	1528, 2045
Bsp1407I	TGTACA	1 cut(s)	1057
Bsp143I	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
Bsp1720I	GCTNAGC	3 cut(s)	2019, 2361, 2439
Bsp19I	CCATGG	1 cut(s)	303
BspACI	CCGC	7 cut(s)	117, 1124, 1775, 2334, 2346, 2369, 2406
BspANI	GGCC	7 cut(s)	885, 979, 1225, 1243, 1652, 1814, 2215
BspCNI	CTCAG	8 cut(s)	118, 998, 1540, 1696, 1913, 1996, 2374, 2452
BspDI	ATCGAT	1 cut(s)	940
BspFNI	CGCG	1 cut(s)	652
BspHI	TCATGA	1 cut(s)	643
BspLI	GGNNCC	3 cut(s)	884, 1244, 2249
BspMAI	CTGCAG	4 cut(s)	1027, 1258, 2311, 2419
BspMI	ACCTGC	3 cut(s)	2143, 2311, 2455
BspOI	GCTAGC	1 cut(s)	288
BspPI	GGATC	5 cut(s)	424, 761, 945, 1422, 2016
BspT104I	TTCGAA	1 cut(s)	574
BsrDI	GCAATG	3 cut(s)	45, 311, 1653
BsrFI	RCCGGY	1 cut(s)	2365
BsrGI	TGTACA	1 cut(s)	1057
BsrI	ACTGG	6 cut(s)	324, 939, 1376, 2140, 2176, 2485
BssAI	RCCGGY	1 cut(s)	2365
BssECI	CCNNGG	6 cut(s)	303, 1446, 1519, 1755, 1872, 2251
BssMI	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
BssT1I	CCWWGG	3 cut(s)	303, 1519, 1872
Bst2UI	CCWGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
Bst4CI	ACNGT	7 cut(s)	11, 19, 373, 953, 1610, 1719, 1989
Bst6I	CTCTTC	3 cut(s)	528, 1152, 1454
BstAPI	GCANNNNNTGC	3 cut(s)	1037, 2276, 2414
BstAUI	TGTACA	1 cut(s)	1057
BstBAI	YACGTR	1 cut(s)	1237
BstBI	TTCGAA	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	303
BstF5I	GGATG	3 cut(s)	157, 448, 1057
BstFNI	CGCG	1 cut(s)	652
BstHHI	GCGC	2 cut(s)	652, 1355
BstKTI	GATC	8 cut(s)	419, 643, 769, 900, 940, 1417, 1438, 2011
BstMAI	GTCTC	3 cut(s)	8, 530, 1456
BstMBI	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
BstMCI	CGRYCG	1 cut(s)	1779
BstNI	CCWGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
BstNSI	RCATGY	3 cut(s)	1595, 1787, 2018
BstSCI	CCNGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
BstSFI	CTRYAG	6 cut(s)	144, 1023, 1254, 2307, 2415, 2427
BstUI	CGCG	1 cut(s)	652
BstV2I	GAAGAC	1 cut(s)	1562
BstX2I	RGATCY	3 cut(s)	416, 766, 1414
BstYI	RGATCY	3 cut(s)	416, 766, 1414
Bsu15I	ATCGAT	1 cut(s)	940
BsuI	GTATCC	1 cut(s)	418
BsuRI	GGCC	7 cut(s)	885, 979, 1225, 1243, 1652, 1814, 2215
BsuTUI	ATCGAT	1 cut(s)	940
BtgI	CCRYGG	1 cut(s)	303
BtsCI	GGATG	3 cut(s)	157, 448, 1057
BtsI	GCAGTG	2 cut(s)	295, 2316
BtsIMutI	CAGTG	4 cut(s)	16, 295, 2183, 2316
BveI	ACCTGC	3 cut(s)	2143, 2311, 2455
CaiI	CAGNNNCTG	1 cut(s)	2414
CciI	TCATGA	1 cut(s)	643
CfoI	GCGC	2 cut(s)	652, 1355
Cfr10I	RCCGGY	1 cut(s)	2365
Cfr13I	GGNCC	4 cut(s)	883, 1242, 1813, 2247
ClaI	ATCGAT	1 cut(s)	940
CsiI	ACCWGGT	1 cut(s)	1564
Csp6I	GTAC	4 cut(s)	850, 1058, 1547, 2300
CspCI	CAANNNNNGTGG	2 cut(s)	2159, 2194
CviQI	GTAC	4 cut(s)	850, 1058, 1547, 2300
DpnI	GATC	8 cut(s)	418, 642, 768, 899, 939, 1416, 1437, 2010
DpnII	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
DraI	TTTAAA	1 cut(s)	948
EaeI	YGGCCR	1 cut(s)	2213
Eam1104I	CTCTTC	3 cut(s)	528, 1152, 1454
EarI	CTCTTC	3 cut(s)	528, 1152, 1454
Eco130I	CCWWGG	3 cut(s)	303, 1519, 1872
Eco147I	AGGCCT	1 cut(s)	1225
Eco32I	GATATC	2 cut(s)	50, 1516
Eco47I	GGWCC	1 cut(s)	2247
Eco57I	CTGAAG	5 cut(s)	384, 1023, 1029, 2034, 2094
Eco72I	CACGTG	1 cut(s)	1237
Eco88I	CYCGRG	1 cut(s)	1808
EcoO109I	RGGNCCY	2 cut(s)	1242, 1813
EcoRII	CCWGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
EcoRV	GATATC	2 cut(s)	50, 1516
EcoT14I	CCWWGG	3 cut(s)	303, 1519, 1872
EcoT22I	ATGCAT	2 cut(s)	184, 2281
ErhI	CCWWGG	3 cut(s)	303, 1519, 1872
FalI	AAGNNNNNCTT	2 cut(s)	1027, 1059
FaqI	GGGAC	2 cut(s)	857, 2250
FauI	CCCGC	2 cut(s)	2341, 2353
FauNDI	CATATG	3 cut(s)	235, 808, 2281
FokI	GGATG	3 cut(s)	144, 455, 1064
FspBI	CTAG	3 cut(s)	285, 596, 1401
GlaI	GCGC	2 cut(s)	651, 1354
HaeIII	GGCC	7 cut(s)	885, 979, 1225, 1243, 1652, 1814, 2215
HapII	CCGG	2 cut(s)	2366, 2453
HhaI	GCGC	2 cut(s)	652, 1355
Hin6I	GCGC	2 cut(s)	650, 1353
HinP1I	GCGC	2 cut(s)	650, 1353
HincII	GTYRAC	1 cut(s)	721
HindII	GTYRAC	1 cut(s)	721
HindIII	AAGCTT	1 cut(s)	2208
HpaII	CCGG	2 cut(s)	2366, 2453
HphI	GGTGA	5 cut(s)	182, 1316, 1469, 1554, 2124
Hpy166II	GTNNAC	3 cut(s)	293, 721, 1058
Hpy8I	GTNNAC	3 cut(s)	293, 721, 1058
HpyAV	CCTTC	5 cut(s)	538, 777, 1295, 1993, 2139
HpyCH4III	ACNGT	7 cut(s)	11, 19, 373, 953, 1610, 1719, 1989
HpyCH4IV	ACGT	1 cut(s)	1236
HpySE526I	ACGT	1 cut(s)	1236
HspAI	GCGC	2 cut(s)	650, 1353
KroI	GCCGGC	1 cut(s)	2365
KroNI	GCCGGC	1 cut(s)	2367
Kzo9I	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
LmnI	GCTCC	6 cut(s)	136, 145, 1675, 2040, 2153, 2455
LweI	GCATC	8 cut(s)	139, 256, 317, 385, 577, 1066, 1328, 1959
MabI	ACCWGGT	1 cut(s)	1564
MaeI	CTAG	3 cut(s)	285, 596, 1401
MaeII	ACGT	1 cut(s)	1236
MaeIII	GTNAC	3 cut(s)	706, 1304, 1833
MalI	GATC	8 cut(s)	418, 642, 768, 899, 939, 1416, 1437, 2010
MboI	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
MfeI	CAATTG	1 cut(s)	1790
MflI	RGATCY	3 cut(s)	416, 766, 1414
MhlI	GDGCHC	2 cut(s)	1528, 2045
MlsI	TGGCCA	1 cut(s)	2215
MluNI	TGGCCA	1 cut(s)	2215
MlyI	GAGTC	2 cut(s)	1838, 1901
MmeI	TCCRAC	4 cut(s)	364, 793, 1113, 1920
Mox20I	TGGCCA	1 cut(s)	2215
Mph1103I	ATGCAT	2 cut(s)	184, 2281
MroNI	GCCGGC	1 cut(s)	2365
MroXI	GAANNNNTTC	1 cut(s)	373
MscI	TGGCCA	1 cut(s)	2215
MslI	CAYNNNNRTG	1 cut(s)	225
Msp20I	TGGCCA	1 cut(s)	2215
MspA1I	CMGCKG	4 cut(s)	251, 2186, 2411, 2426
MspI	CCGG	2 cut(s)	2366, 2453
MspR9I	CCNGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
MunI	CAATTG	1 cut(s)	1790
Mva1269I	GAATGC	3 cut(s)	1678, 1747, 1802
MvaI	CCWGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
MvnI	CGCG	1 cut(s)	652
NaeI	GCCGGC	1 cut(s)	2367
NcoI	CCATGG	1 cut(s)	303
NdeI	CATATG	3 cut(s)	235, 808, 2281
NdeII	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
NgoMIV	GCCGGC	1 cut(s)	2365
NheI	GCTAGC	1 cut(s)	284
NlaIV	GGNNCC	3 cut(s)	884, 1244, 2249
NmuCI	GTSAC	1 cut(s)	1304
NsiI	ATGCAT	2 cut(s)	184, 2281
NspI	RCATGY	3 cut(s)	1595, 1787, 2018
NspV	TTCGAA	1 cut(s)	574
PaeI	GCATGC	2 cut(s)	1595, 2018
PaeR7I	CTCGAG	1 cut(s)	1808
PagI	TCATGA	1 cut(s)	643
PaqCI	CACCTGC	1 cut(s)	2143
PceI	AGGCCT	1 cut(s)	1225
PcsI	WCGNNNNNNNCGW	1 cut(s)	1838
PctI	GAATGC	3 cut(s)	1678, 1747, 1802
PdiI	GCCGGC	1 cut(s)	2367
PdmI	GAANNNNTTC	1 cut(s)	373
PfeI	GAWTC	9 cut(s)	86, 200, 215, 332, 919, 1187, 1375, 1474, 1868
PflMI	CCANNNNNTGG	3 cut(s)	167, 254, 934
PleI	GAGTC	2 cut(s)	1837, 1900
PmaCI	CACGTG	1 cut(s)	1237
PmlI	CACGTG	1 cut(s)	1237
PpsI	GAGTC	2 cut(s)	1837, 1900
Ppu21I	YACGTR	1 cut(s)	1237
PshBI	ATTAAT	1 cut(s)	1200
Psp6I	CCWGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
PspCI	CACGTG	1 cut(s)	1237
PspGI	CCWGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
PspN4I	GGNNCC	3 cut(s)	884, 1244, 2249
PspPI	GGNCC	4 cut(s)	883, 1242, 1813, 2247
PspXI	VCTCGAGB	1 cut(s)	1808
PstI	CTGCAG	4 cut(s)	1027, 1258, 2311, 2419
PstNI	CAGNNNCTG	1 cut(s)	2414
PsuI	RGATCY	3 cut(s)	416, 766, 1414
PvuII	CAGCTG	4 cut(s)	251, 2186, 2411, 2426
RsaI	GTAC	4 cut(s)	851, 1059, 1548, 2301
RsaNI	GTAC	4 cut(s)	850, 1058, 1547, 2300
RseI	CAYNNNNRTG	1 cut(s)	225
Sau3AI	GATC	8 cut(s)	416, 640, 766, 897, 937, 1414, 1435, 2008
Sau96I	GGNCC	4 cut(s)	883, 1242, 1813, 2247
SchI	GAGTC	2 cut(s)	1838, 1901
ScrFI	CCNGG	6 cut(s)	168, 517, 1448, 1566, 1756, 2253
SduI	GDGCHC	2 cut(s)	1528, 2045
SexAI	ACCWGGT	1 cut(s)	1564
SfaNI	GCATC	8 cut(s)	139, 256, 317, 385, 577, 1066, 1328, 1959
SfcI	CTRYAG	6 cut(s)	144, 1023, 1254, 2307, 2415, 2427
Sfr274I	CTCGAG	1 cut(s)	1808
SfuI	TTCGAA	1 cut(s)	574
SinI	GGWCC	1 cut(s)	2247
SlaI	CTCGAG	1 cut(s)	1808
SmiMI	CAYNNNNRTG	1 cut(s)	225
SmlI	CTYRAG	2 cut(s)	1808, 2205
SmoI	CTYRAG	2 cut(s)	1808, 2205
SphI	GCATGC	2 cut(s)	1595, 2018
SseBI	AGGCCT	1 cut(s)	1225
SsiI	CCGC	7 cut(s)	117, 1124, 1775, 2334, 2346, 2369, 2406
SspI	AATATT	2 cut(s)	100, 912
SspMI	CTAG	3 cut(s)	285, 596, 1401
StuI	AGGCCT	1 cut(s)	1225
StyD4I	CCNGG	6 cut(s)	166, 515, 1446, 1564, 1754, 2251
StyI	CCWWGG	3 cut(s)	303, 1519, 1872
TaaI	ACNGT	7 cut(s)	11, 19, 373, 953, 1610, 1719, 1989
TaiI	ACGT	1 cut(s)	1239
TaqI	TCGA	7 cut(s)	574, 940, 1153, 1185, 1779, 1809, 1827
TatI	WGTACW	1 cut(s)	1057
TauI	GCSGC	2 cut(s)	2372, 2408
TfiI	GAWTC	9 cut(s)	86, 200, 215, 332, 919, 1187, 1375, 1474, 1868
TscAI	CASTG	4 cut(s)	16, 295, 2183, 2316
TseFI	GTSAC	1 cut(s)	1304
Tsp45I	GTSAC	1 cut(s)	1304
TspDTI	ATGAA	6 cut(s)	312, 366, 702, 876, 1347, 2024
TspRI	CASTG	4 cut(s)	16, 295, 2183, 2316
Van91I	CCANNNNNTGG	3 cut(s)	167, 254, 934
VpaK11BI	GGWCC	1 cut(s)	2247
VspI	ATTAAT	1 cut(s)	1200
XapI	RAATTY	4 cut(s)	123, 570, 863, 1557
XceI	RCATGY	3 cut(s)	1595, 1787, 2018
XcmI	CCANNNNNNNNNTGG	2 cut(s)	164, 2183
XhoI	CTCGAG	1 cut(s)	1808
XmnI	GAANNNNTTC	1 cut(s)	373
XspI	CTAG	3 cut(s)	285, 596, 1401
Zsp2I	ATGCAT	2 cut(s)	184, 2281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.