Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

9205743 .. 9208919

Gene Structure 3177 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00168310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGCAGTGGCAGACTTCTTCGCCGGAGAGATTATCACGGAGCTCCTGAAGAACCTGGTAGCCATATCCAAAAAATCATGGCAGTTCAAAGACAGCGCCAACGGCCTCAAATACTCCATAGAACAGTTCCTCCCCATAATCCAAGAAATCAAGTACTCCGGCGTCGAGTTGCCGGCCATCCGCCAGTCTCAGCTCGACCGTCTCTCGGAGACTCTCCGGCAGGGCCATGAGCTGTCCCACAAGGTCCTCCTCTCCTCTCGCTACAACATTTACAAGAACCTCCAATATGCCCGCAAGATGGAGAGGATCGAAAAGACCGTCGCCAAGTTCATCCAGGGCCCATTGCAGGCCCATATCTTGGCCGACGTCCACCACATAAGGTTCCAGACCACCGAGCGCTTCGACCGCCTTGAAGGCTCCAGCTACCGCCTGGAGCAGAGGCTCGGCGCTATGAAGATTGGAGCTTGCAGTGGCGGCGGAGGTGTGTCGTGGGTGGAAGAGGCTGTGAGGCGCGTGGACGAGGAGGGTCGGATGCAATGGGACAGCAGCAGTGAGTTGGTTTTGGGCTTGGGTTTCGGGTTGAAGAAAGTCAAGGAAATGGTTCTTGGCAGAGATGACTTGTTCGTTGGGATTAGTGGGATTGGCGGCTCGGGAAAAACCACTCTTGTTAAGGAGTTGATTAAGGATGATCATGTTCGATCTTACTTTGGTGAGAGGATCCTGTTCCTAACGGTGTCACAATCTCCGGATATAGAGGATTTGAAGGCCAAGATCTTGGGGTTCATTATGGGGAGTCAAGTTTTGAGTCCCAGTTCTGTGCTTCCTCAAATGAACTTTCAGTTTGAATGGAGAAACCCAACAAAGACCCTGGTGGTTTTGGATGATGTTTGGTCACTTTCAACGCTTCAGCCACTGCTCTTCAACATTCCTGGTTGTAAAACCCTTGTGGTTTCACGTTTCAAATTCCAAACAGTAACAAAAACCACCTATGACGTGGAGTTGCTGGGTGAAGATGAAGCAGTCTCTCTGTTTTGCCACTCTGCTTTTGGCCAAAAGTTCATTCCTCCTGGTGCTGATAAGAAATTGGTCAAGCAGATTGTTGATGAGTGTAAGAGGCTTCCTTTGGCTCTCAAAGTGATTGGGTCATCTTTACGAGACCAGCCTGAAATGATTTGGGCAAATGCAAGCAAAAGGTTGTCACGAGGGGAAAGCATCGGCGAGTCGCATGAGAACAACCTACTTGATCGGATGGCAATCAGTGTGAAATGCTTAAGCCCCAGAGTCAGGGAATGTTTCCTGGATTTGGGTGCCTTTCCAGAAGACAAGAAGATCCCACTGGATGTTCTTATCAACATTTGGGTTGAAACCCATGATCACATTGATGAAGAACAAGCCTTTGCCATTCTTCACGAGCTTTCGGAAAAGAATCTTGTCACCCTAGTAAAAGATGCAAGAGATGGAGACATATATAGCAGTTACTATGACATCTCAGTTACACAACATGATGTCTTGCGAGACCTTGCTCTTCATTTGAGTAATAATGGCTCTGTAAATCAACGCAGCCGATTACTGATGCCAAGAAGAGAGATAGAACTTCCAAGAGAATGGGACAGGCATTCTGATGAACCATTTCAAGCTCGAATTGTGTCAATTCATACAGGTGAAATGACAGAGATGGATTGGTTCGAGATGGAATTCCCCAAGGCTGAAGTGCTCATCTTGAATTTCGCCTCCAACGAGTACTTGTTACCTCCTTTCATTCGTAACATGCCAAAGTTGAAGGCATTGATCTTGATGAATTACAGTGCATCCAATGCAATTCTCCATGGTTTTTCTGTCTTGTCAAATTTGGGCAACTTGAGAAGCCTATGGCTTGAAAAAGTCTCGGTCCCTCCCTTATCCCCTGCTACCTTCCCTTCCAAAAAATTACGCAAGATTTCGTTAATCTTGTGTAAGATTAATAACAGCCTCCATCAATCAGTATTTCCTCGGCTCTTAGAACTCACAATTGATCATTGTGATGATTTGTTCGAGCTACCTTCAAGCATTTGTGGGATGCATTTGCTTTACAATCTAAGCATAACCAACTGTCACCATCTATGGCAACTGCCAGCGGATTTGAGTAAGCTGAAATCTCTACAAATCCTGAGGCTATATGCATGCCCATATCTGCAAAAACTTCCTCCTGACATTTGTGAGCTCTGTTGCTTGAAGTATCTAAACATTTCCCAATGCTGTAGTTTGAGGGGCCTTCCCTTAGACATTTATAAGTTGGCAAGTTTGGAAAAGATTGACATGAGGGAATGCTCACAAATCAGGAACCTTCCAAACTCTACAGCAGCTCCACCATCTCTTGGTCGTGTAGTATGTGATGAAGAGATTTCTTGGTTCTGGAAGAAGGCATTGCCAGACCTTCACGTTCAGGTTGCAGAGAAACGTTTCGATCTTGACTGGCTTGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

821

Amino Acids

93.43

Weight (kDa)

6.45

Isoelectric Point (pI)

50.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	9 - 129	2.7e-13	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	196 - 351	1.1e-15	NB-ARC domain
LRR_14	PF23598	642 - 769	4.2e-09	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2268
AatII	GACGTC	1 cut(s)	369
AccB1I	GGYRCC	1 cut(s)	1307
AccB7I	CCANNNNNTGG	2 cut(s)	358, 2354
AccII	CGCG	1 cut(s)	513
AccIII	TCCGGA	1 cut(s)	745
AciI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2114
AclI	AACGTT	1 cut(s)	2437
AclWI	GGATC	4 cut(s)	314, 712, 725, 1324
AcoI	YGGCCR	3 cut(s)	174, 360, 1048
AcsI	RAATTY	4 cut(s)	962, 1694, 1723, 1846
AcuI	CTGAAG	3 cut(s)	68, 890, 1728
AcyI	GRCGYC	2 cut(s)	162, 366
AfaI	GTAC	2 cut(s)	155, 1742
AfeI	AGCGCT	1 cut(s)	398
AfiI	CCNNNNNNNGG	7 cut(s)	205, 298, 346, 358, 1284, 1303, 2354
AflII	CTTAAG	1 cut(s)	1270
AjiI	CACGTC	1 cut(s)	994
AjnI	CCWGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
AjuI	GAANNNNNNNTTGG	4 cut(s)	588, 620, 1044, 1076
AloI	GAACNNNNNNTCC	2 cut(s)	114, 146
Alw21I	GWGCWC	3 cut(s)	45, 1716, 2202
Alw26I	GTCTC	8 cut(s)	192, 203, 206, 1027, 1149, 1455, 1509, 1888
AlwI	GGATC	4 cut(s)	314, 712, 725, 1324
AlwNI	CAGNNNCTG	1 cut(s)	913
Ama87I	CYCGRG	1 cut(s)	649
Aor13HI	TCCGGA	1 cut(s)	745
Aor51HI	AGCGCT	1 cut(s)	398
AoxI	GGCC	9 cut(s)	103, 174, 223, 337, 348, 360, 765, 1048, 2248
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	3 cut(s)	546, 1560, 2339
ApoI	RAATTY	4 cut(s)	962, 1694, 1723, 1846
ArsI	GACNNNNNNTTYG	2 cut(s)	303, 335
AseI	ATTAAT	1 cut(s)	1959
Asp700I	GAANNNNTTC	3 cut(s)	600, 1629, 2438
AspLEI	GCGC	4 cut(s)	98, 399, 449, 513
AspS9I	GGNCC	7 cut(s)	223, 244, 337, 338, 349, 1888, 2248
AsuHPI	GGTGA	5 cut(s)	722, 1019, 1426, 1673, 2084
AvaI	CYCGRG	1 cut(s)	649
AvaII	GGWCC	2 cut(s)	244, 1888
AxyI	CCTNAGG	1 cut(s)	2147
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	1 cut(s)	1050
BamHI	GGATCC	1 cut(s)	717
BanI	GGYRCC	1 cut(s)	1307
BanII	GRGCYC	3 cut(s)	45, 341, 2202
BauI	CACGAG	2 cut(s)	1200, 1409
BbsI	GAAGAC	1 cut(s)	1326
Bbv12I	GWGCWC	3 cut(s)	45, 1716, 2202
BbvI	GCAGC	3 cut(s)	558, 1572, 2351
BccI	CCATC	9 cut(s)	185, 292, 1243, 1451, 1669, 1684, 1980, 2103, 2356
BceAI	ACGGC	1 cut(s)	118
BciT130I	CCWGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
BclI	TGATCA	3 cut(s)	688, 1372, 2011
BcoDI	GTCTC	8 cut(s)	192, 203, 206, 1027, 1149, 1455, 1509, 1888
BfaI	CTAG	1 cut(s)	1439
BfmI	CTRYAG	2 cut(s)	2236, 2334
BfoI	RGCGCY	3 cut(s)	99, 400, 450
BfrI	CTTAAG	1 cut(s)	1270
BglI	GCCNNNNNGGC	1 cut(s)	414
BglII	AGATCT	1 cut(s)	771
BisI	GCNGC	5 cut(s)	475, 547, 646, 1561, 2340
BlsI	GCNGC	5 cut(s)	476, 548, 647, 1562, 2341
BmcAI	AGTACT	2 cut(s)	155, 1742
Bme1390I	CCNGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
Bme18I	GGWCC	2 cut(s)	244, 1888
BmeT110I	CYCGRG	1 cut(s)	649
BmgBI	CACGTC	1 cut(s)	994
BmgT120I	GGNCC	7 cut(s)	223, 244, 337, 338, 349, 1888, 2248
BmiI	GGNNCC	8 cut(s)	339, 383, 418, 719, 1309, 1890, 2249, 2321
BmrFI	CCNGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
BmrI	ACTGGG	1 cut(s)	804
BmsI	GCATC	6 cut(s)	522, 1221, 1438, 1563, 1817, 2046
BmuI	ACTGGG	1 cut(s)	804
BpiI	GAAGAC	1 cut(s)	1326
BpmI	CTGGAG	2 cut(s)	403, 452
BpuEI	CTTGAG	1 cut(s)	1879
BsaBI	GATNNNNATC	1 cut(s)	1253
BsaHI	GRCGYC	2 cut(s)	162, 366
BsaI	GGTCTC	2 cut(s)	1149, 1509
BsaJI	CCNNGG	5 cut(s)	334, 867, 1701, 1825, 1988
BsaWI	WCCGGW	1 cut(s)	745
BsaXI	ACNNNNNCTCC	4 cut(s)	114, 144, 2326, 2356
Bsc4I	CCNNNNNNNGG	7 cut(s)	205, 298, 346, 358, 1284, 1303, 2354
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	4 cut(s)	184, 810, 1341, 2456
Bse21I	CCTNAGG	1 cut(s)	2147
Bse3DI	GCAATG	3 cut(s)	341, 542, 2402
Bse8I	GATNNNNATC	1 cut(s)	1253
BseAI	TCCGGA	1 cut(s)	745
BseBI	CCWGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
BseDI	CCNNGG	5 cut(s)	334, 867, 1701, 1825, 1988
BseGI	GGATG	9 cut(s)	177, 330, 537, 691, 886, 1254, 1345, 1808, 2061
BseJI	GATNNNNATC	1 cut(s)	1253
BseLI	CCNNNNNNNGG	7 cut(s)	205, 298, 346, 358, 1284, 1303, 2354
BseMI	GCAATG	3 cut(s)	341, 542, 2402
BseMII	CTCAG	3 cut(s)	203, 1503, 2138
BseNI	ACTGG	4 cut(s)	184, 810, 1341, 2456
BseRI	GAGGAG	3 cut(s)	239, 244, 536
BseSI	GKGCMC	1 cut(s)	341
BseXI	GCAGC	3 cut(s)	558, 1572, 2351
BseYI	CCCAGC	1 cut(s)	1003
Bsh1236I	CGCG	1 cut(s)	513
Bsh1285I	CGRYCG	2 cut(s)	199, 406
BshFI	GGCC	9 cut(s)	105, 176, 225, 339, 350, 362, 767, 1050, 2250
BshNI	GGYRCC	1 cut(s)	1307
BsiEI	CGRYCG	2 cut(s)	199, 406
BsiHKAI	GWGCWC	3 cut(s)	45, 1716, 2202
BsiHKCI	CYCGRG	1 cut(s)	649
BsiSI	CCGG	5 cut(s)	24, 159, 173, 217, 746
BslFI	GGGAC	5 cut(s)	220, 554, 792, 1622, 1874
BslI	CCNNNNNNNGG	7 cut(s)	205, 298, 346, 358, 1284, 1303, 2354
BsmAI	GTCTC	8 cut(s)	192, 203, 206, 1027, 1149, 1455, 1509, 1888
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	5 cut(s)	220, 554, 792, 1622, 1874
BsmI	GAATGC	2 cut(s)	1615, 2309
BsnI	GGCC	9 cut(s)	105, 176, 225, 339, 350, 362, 767, 1050, 2250
Bso31I	GGTCTC	2 cut(s)	1149, 1509
BsoBI	CYCGRG	1 cut(s)	649
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	4 cut(s)	45, 341, 1716, 2202
Bsp13I	TCCGGA	1 cut(s)	745
Bsp19I	CCATGG	1 cut(s)	1825
BspACI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2114
BspANI	GGCC	9 cut(s)	105, 176, 225, 339, 350, 362, 767, 1050, 2250
BspCNI	CTCAG	3 cut(s)	202, 1502, 2139
BspEI	TCCGGA	1 cut(s)	745
BspFNI	CGCG	1 cut(s)	513
BspLI	GGNNCC	8 cut(s)	339, 383, 418, 719, 1309, 1890, 2249, 2321
BspPI	GGATC	4 cut(s)	314, 712, 725, 1324
BspQI	GCTCTTC	2 cut(s)	923, 1530
BspT107I	GGYRCC	1 cut(s)	1307
BspTI	CTTAAG	1 cut(s)	1270
BspTNI	GGTCTC	2 cut(s)	1149, 1509
BsrDI	GCAATG	3 cut(s)	341, 542, 2402
BsrFI	RCCGGY	1 cut(s)	172
BsrI	ACTGG	4 cut(s)	184, 810, 1341, 2456
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	5 cut(s)	334, 867, 1701, 1825, 1988
BssNI	GRCGYC	2 cut(s)	162, 366
BssSI	CACGAG	2 cut(s)	1200, 1409
BssT1I	CCWWGG	2 cut(s)	1701, 1825
Bst2BI	CACGAG	2 cut(s)	1200, 1409
Bst2UI	CCWGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
Bst4CI	ACNGT	7 cut(s)	126, 200, 319, 733, 973, 1805, 2090
Bst6I	CTCTTC	5 cut(s)	492, 923, 1530, 1576, 2370
BstACI	GRCGYC	2 cut(s)	162, 366
BstAFI	CTTAAG	1 cut(s)	1270
BstAPI	GCANNNNNTGC	1 cut(s)	1814
BstC8I	GCNNGC	7 cut(s)	174, 292, 348, 466, 1186, 2112, 2161
BstDEI	CTNAG	6 cut(s)	189, 1489, 1996, 2075, 2147, 2257
BstDSI	CCRYGG	1 cut(s)	1825
BstF5I	GGATG	9 cut(s)	177, 330, 537, 691, 886, 1254, 1345, 1808, 2061
BstFNI	CGCG	1 cut(s)	513
BstH2I	RGCGCY	3 cut(s)	99, 400, 450
BstHHI	GCGC	4 cut(s)	98, 399, 449, 513
BstMAI	GTCTC	8 cut(s)	192, 203, 206, 1027, 1149, 1455, 1509, 1888
BstMCI	CGRYCG	2 cut(s)	199, 406
BstMWI	GCNNNNNNNGC	5 cut(s)	102, 405, 414, 474, 1814
BstNI	CCWGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
BstNSI	RCATGY	2 cut(s)	1771, 2163
BstSCI	CCNGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
BstSFI	CTRYAG	2 cut(s)	2236, 2334
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	1 cut(s)	513
BstV1I	GCAGC	3 cut(s)	558, 1572, 2351
BstV2I	GAAGAC	1 cut(s)	1326
BstX2I	RGATCY	3 cut(s)	717, 771, 1329
BstXI	CCANNNNNNTGG	2 cut(s)	775, 1605
BstYI	RGATCY	3 cut(s)	717, 771, 1329
Bsu36I	CCTNAGG	1 cut(s)	2147
BsuRI	GGCC	9 cut(s)	105, 176, 225, 339, 350, 362, 767, 1050, 2250
BtgI	CCRYGG	1 cut(s)	1825
BtrI	CACGTC	1 cut(s)	994
BtsCI	GGATG	9 cut(s)	177, 330, 537, 691, 886, 1254, 1345, 1808, 2061
BtsI	GCAGTG	4 cut(s)	12, 475, 556, 911
BtsIMutI	CAGTG	7 cut(s)	12, 475, 556, 911, 1264, 1334, 1810
Cac8I	GCNNGC	7 cut(s)	174, 292, 348, 466, 1186, 2112, 2161
CaiI	CAGNNNCTG	1 cut(s)	913
CfoI	GCGC	4 cut(s)	98, 399, 449, 513
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	7 cut(s)	223, 244, 337, 338, 349, 1888, 2248
CseI	GACGC	1 cut(s)	151
CsiI	ACCWGGT	1 cut(s)	54
Csp6I	GTAC	2 cut(s)	154, 1741
CviQI	GTAC	2 cut(s)	154, 1741
DdeI	CTNAG	6 cut(s)	189, 1489, 1996, 2075, 2147, 2257
EaeI	YGGCCR	3 cut(s)	174, 360, 1048
Eam1104I	CTCTTC	5 cut(s)	492, 923, 1530, 1576, 2370
EarI	CTCTTC	5 cut(s)	492, 923, 1530, 1576, 2370
EciI	GGCGGA	2 cut(s)	170, 492
Ecl136II	GAGCTC	2 cut(s)	43, 2200
Eco130I	CCWWGG	2 cut(s)	1701, 1825
Eco24I	GRGCYC	3 cut(s)	45, 341, 2202
Eco31I	GGTCTC	2 cut(s)	1149, 1509
Eco47I	GGWCC	2 cut(s)	244, 1888
Eco47III	AGCGCT	1 cut(s)	398
Eco53kI	GAGCTC	2 cut(s)	43, 2200
Eco57I	CTGAAG	3 cut(s)	68, 890, 1728
Eco81I	CCTNAGG	1 cut(s)	2147
Eco88I	CYCGRG	1 cut(s)	649
EcoICRI	GAGCTC	2 cut(s)	43, 2200
EcoO109I	RGGNCCY	3 cut(s)	244, 337, 2248
EcoRI	GAATTC	1 cut(s)	1694
EcoRII	CCWGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
EcoT14I	CCWWGG	2 cut(s)	1701, 1825
EcoT22I	ATGCAT	2 cut(s)	2061, 2161
EcoT38I	GRGCYC	3 cut(s)	45, 341, 2202
ErhI	CCWWGG	2 cut(s)	1701, 1825
Esp3I	CGTCTC	1 cut(s)	206
FaqI	GGGAC	5 cut(s)	220, 554, 792, 1622, 1874
FauI	CCCGC	1 cut(s)	299
FbaI	TGATCA	3 cut(s)	688, 1372, 2011
Fnu4HI	GCNGC	5 cut(s)	475, 547, 646, 1561, 2340
FokI	GGATG	9 cut(s)	164, 317, 544, 698, 893, 1261, 1352, 1795, 2068
FriOI	GRGCYC	3 cut(s)	45, 341, 2202
Fsp4HI	GCNGC	5 cut(s)	475, 547, 646, 1561, 2340
FspBI	CTAG	1 cut(s)	1439
GlaI	GCGC	4 cut(s)	97, 398, 448, 512
GluI	GCNGC	5 cut(s)	475, 547, 646, 1561, 2340
GsaI	CCCAGC	1 cut(s)	1007
GsuI	CTGGAG	2 cut(s)	403, 452
HaeII	RGCGCY	3 cut(s)	99, 400, 450
HaeIII	GGCC	9 cut(s)	105, 176, 225, 339, 350, 362, 767, 1050, 2250
HapII	CCGG	5 cut(s)	24, 159, 173, 217, 746
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	4 cut(s)	98, 399, 449, 513
Hin1I	GRCGYC	2 cut(s)	162, 366
Hin6I	GCGC	4 cut(s)	96, 397, 447, 511
HinP1I	GCGC	4 cut(s)	96, 397, 447, 511
HinfI	GANTC	6 cut(s)	211, 793, 805, 1220, 1281, 1426
HpaII	CCGG	5 cut(s)	24, 159, 173, 217, 746
HphI	GGTGA	5 cut(s)	722, 1019, 1426, 1673, 2084
Hpy166II	GTNNAC	2 cut(s)	370, 517
Hpy188I	TCNGA	5 cut(s)	208, 531, 1248, 1420, 1621
Hpy8I	GTNNAC	2 cut(s)	370, 517
Hpy99I	CGWCG	3 cut(s)	167, 323, 368
HpyCH4III	ACNGT	7 cut(s)	126, 200, 319, 733, 973, 1805, 2090
HpyCH4IV	ACGT	5 cut(s)	366, 955, 993, 2418, 2437
HpyF10VI	GCNNNNNNNGC	5 cut(s)	102, 405, 414, 474, 1814
HpyF3I	CTNAG	6 cut(s)	189, 1489, 1996, 2075, 2147, 2257
HpySE526I	ACGT	5 cut(s)	366, 955, 993, 2418, 2437
Hsp92I	GRCGYC	2 cut(s)	162, 366
HspAI	GCGC	4 cut(s)	96, 397, 447, 511
Kpn2I	TCCGGA	1 cut(s)	745
KroI	GCCGGC	1 cut(s)	172
KroNI	GCCGGC	1 cut(s)	174
Ksp22I	TGATCA	3 cut(s)	688, 1372, 2011
LguI	GCTCTTC	2 cut(s)	923, 1530
LmnI	GCTCC	6 cut(s)	40, 48, 422, 433, 461, 2347
Lsp1109I	GCAGC	3 cut(s)	558, 1572, 2351
LweI	GCATC	6 cut(s)	522, 1221, 1438, 1563, 1817, 2046
MabI	ACCWGGT	1 cut(s)	54
MaeI	CTAG	1 cut(s)	1439
MaeII	ACGT	5 cut(s)	366, 955, 993, 2418, 2437
MfeI	CAATTG	1 cut(s)	2007
MflI	RGATCY	3 cut(s)	717, 771, 1329
MhlI	GDGCHC	4 cut(s)	45, 341, 1716, 2202
MlsI	TGGCCA	1 cut(s)	1050
MluNI	TGGCCA	1 cut(s)	1050
MlyI	GAGTC	5 cut(s)	205, 802, 814, 1229, 1290
MmeI	TCCRAC	2 cut(s)	509, 1758
Mox20I	TGGCCA	1 cut(s)	1050
Mph1103I	ATGCAT	2 cut(s)	2061, 2161
MroI	TCCGGA	1 cut(s)	745
MroNI	GCCGGC	1 cut(s)	172
MroXI	GAANNNNTTC	3 cut(s)	600, 1629, 2438
MscI	TGGCCA	1 cut(s)	1050
MseI	TTAA	5 cut(s)	669, 681, 1271, 1943, 1959
MslI	CAYNNNNRTG	4 cut(s)	1380, 1620, 1659, 2019
Msp20I	TGGCCA	1 cut(s)	1050
MspA1I	CMGCKG	1 cut(s)	2114
MspCI	CTTAAG	1 cut(s)	1270
MspI	CCGG	5 cut(s)	24, 159, 173, 217, 746
MspR9I	CCNGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
MunI	CAATTG	1 cut(s)	2007
Mva1269I	GAATGC	2 cut(s)	1615, 2309
MvaI	CCWGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
MvnI	CGCG	1 cut(s)	513
MwoI	GCNNNNNNNGC	5 cut(s)	102, 405, 414, 474, 1814
NaeI	GCCGGC	1 cut(s)	174
NcoI	CCATGG	1 cut(s)	1825
NgoMIV	GCCGGC	1 cut(s)	172
NlaIV	GGNNCC	8 cut(s)	339, 383, 418, 719, 1309, 1890, 2249, 2321
NmeAIII	GCCGAG	2 cut(s)	423, 1969
NmuCI	GTSAC	5 cut(s)	735, 891, 1197, 1432, 2090
NsiI	ATGCAT	2 cut(s)	2061, 2161
NspI	RCATGY	2 cut(s)	1771, 2163
PaeI	GCATGC	1 cut(s)	2163
PciSI	GCTCTTC	2 cut(s)	923, 1530
PcsI	WCGNNNNNNNCGW	2 cut(s)	315, 1734
PctI	GAATGC	2 cut(s)	1615, 2309
PdiI	GCCGGC	1 cut(s)	174
PdmI	GAANNNNTTC	3 cut(s)	600, 1629, 2438
PfeI	GAWTC	1 cut(s)	1426
PflMI	CCANNNNNTGG	2 cut(s)	358, 2354
PfoI	TCCNGGA	1 cut(s)	1296
PkrI	GCNGC	5 cut(s)	476, 548, 647, 1562, 2341
PleI	GAGTC	5 cut(s)	205, 801, 813, 1228, 1289
PpsI	GAGTC	5 cut(s)	205, 801, 813, 1228, 1289
PpuMI	RGGWCCY	1 cut(s)	244
PshBI	ATTAAT	1 cut(s)	1959
PsiI	TTATAA	1 cut(s)	2268
Psp124BI	GAGCTC	2 cut(s)	45, 2202
Psp1406I	AACGTT	1 cut(s)	2437
Psp5II	RGGWCCY	1 cut(s)	244
Psp6I	CCWGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
PspFI	CCCAGC	1 cut(s)	1003
PspGI	CCWGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
PspN4I	GGNNCC	8 cut(s)	339, 383, 418, 719, 1309, 1890, 2249, 2321
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	7 cut(s)	223, 244, 337, 338, 349, 1888, 2248
PspPPI	RGGWCCY	1 cut(s)	244
PstNI	CAGNNNCTG	1 cut(s)	913
PsuI	RGATCY	3 cut(s)	717, 771, 1329
RsaI	GTAC	2 cut(s)	155, 1742
RsaNI	GTAC	2 cut(s)	154, 1741
RseI	CAYNNNNRTG	4 cut(s)	1380, 1620, 1659, 2019
SacI	GAGCTC	2 cut(s)	45, 2202
SapI	GCTCTTC	2 cut(s)	923, 1530
SaqAI	TTAA	5 cut(s)	669, 681, 1271, 1943, 1959
SatI	GCNGC	5 cut(s)	475, 547, 646, 1561, 2340
Sau96I	GGNCC	7 cut(s)	223, 244, 337, 338, 349, 1888, 2248
ScaI	AGTACT	2 cut(s)	155, 1742
SchI	GAGTC	5 cut(s)	205, 802, 814, 1229, 1290
ScrFI	CCNGG	7 cut(s)	56, 335, 431, 869, 930, 1068, 1298
SduI	GDGCHC	4 cut(s)	45, 341, 1716, 2202
SexAI	ACCWGGT	1 cut(s)	54
SfaNI	GCATC	6 cut(s)	522, 1221, 1438, 1563, 1817, 2046
SfcI	CTRYAG	2 cut(s)	2236, 2334
SinI	GGWCC	2 cut(s)	244, 1888
SmiMI	CAYNNNNRTG	4 cut(s)	1380, 1620, 1659, 2019
SmlI	CTYRAG	2 cut(s)	1270, 1858
SmoI	CTYRAG	2 cut(s)	1270, 1858
SphI	GCATGC	1 cut(s)	2163
SsiI	CCGC	8 cut(s)	181, 292, 406, 427, 474, 477, 645, 2114
SspMI	CTAG	1 cut(s)	1439
SstI	GAGCTC	2 cut(s)	45, 2202
StyD4I	CCNGG	7 cut(s)	54, 333, 429, 867, 928, 1066, 1296
StyI	CCWWGG	2 cut(s)	1701, 1825
TaaI	ACNGT	7 cut(s)	126, 200, 319, 733, 973, 1805, 2090
TaiI	ACGT	5 cut(s)	369, 958, 996, 2421, 2440
TaqI	TCGA	9 cut(s)	165, 195, 309, 402, 697, 1639, 1686, 2031, 2442
TaqII	GACCGA	1 cut(s)	1876
TatI	WGTACW	2 cut(s)	153, 1740
TauI	GCSGC	2 cut(s)	477, 648
TfiI	GAWTC	1 cut(s)	1426
Tru1I	TTAA	5 cut(s)	669, 681, 1271, 1943, 1959
Tru9I	TTAA	5 cut(s)	669, 681, 1271, 1943, 1959
TscAI	CASTG	7 cut(s)	12, 475, 556, 918, 1264, 1341, 1810
TseFI	GTSAC	5 cut(s)	735, 891, 1197, 1432, 2090
TseI	GCWGC	3 cut(s)	546, 1560, 2339
Tsp45I	GTSAC	5 cut(s)	735, 891, 1197, 1432, 2090
TspGWI	ACGGA	1 cut(s)	53
TspRI	CASTG	7 cut(s)	12, 475, 556, 918, 1264, 1341, 1810
Van91I	CCANNNNNTGG	2 cut(s)	358, 2354
Vha464I	CTTAAG	1 cut(s)	1270
VpaK11BI	GGWCC	2 cut(s)	244, 1888
VspI	ATTAAT	1 cut(s)	1959
XapI	RAATTY	4 cut(s)	962, 1694, 1723, 1846
XceI	RCATGY	2 cut(s)	1771, 2163
XcmI	CCANNNNNNNNNTGG	3 cut(s)	427, 991, 1043
XmnI	GAANNNNTTC	3 cut(s)	600, 1629, 2438
XspI	CTAG	1 cut(s)	1439
ZraI	GACGTC	1 cut(s)	367
ZrmI	AGTACT	2 cut(s)	155, 1742
Zsp2I	ATGCAT	2 cut(s)	2061, 2161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.