Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009528

Physical Location & Seq

Genomic Coordinates Reverse (-)

3122758 .. 3129739

Gene Structure 6982 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1042500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGCAGTGACGGACTTCTTCGTCGGAGAGATCATCACGGAGCTCCTGAGAACCTTAATCGCCATCTCCCGCAAATCATGCCACAGCAGAGGAACCGCCGAGGAACTCAAATCCTTAGTTGAGCAGCTCTTCCCCATAATCCAAGAAATCAAGTACTCCGGCGTCGAGCTACCGGCCGAACGGCAGTCCCAGCTCGACCGCATGTCGGAAGTTCTCCAGAACGGCCTCGAATTGGCCCGAAAGGTCCACGCGACACCGCGCTGGAACTTTTACAGGCACGTCAGATTGGCGAAAAAGATGGAGGCTTTCGAGAAGACAGTGTCCAGATTTCTTCAGGGCCCATTACAGGTCCATATCTTGGCCGACGTCCACCACATAAGGGCGGAGGCGGATCAGCGGTTCGACCGGCTTGATTGGTCGAACCGGATGCTGGAACAGCGGCTTGGGACTTTAAAGATTGGGACTTGCGGAGGCGGGGGTGTCGGTGGCGGGTGGATGGAGGAGGCTGTGAGGAGGGTGGAGGAGGAAGGGCAGATGAAGTGGGAGGTGAGCAGTTCTGTGGTGTTGGGATTGGGGGTGGGGATGAAGACGGTGAAGGGAATGATTTTTGGGAGGGAGGATTTGAGGGTCGTTGGAATTTGTGGGATTGGTGGCTCTGGAAAAACCACTCTCGCTAGAGAGCTTTGCAAGGATGATCAAGTTAGATCTTATTTCAGTGAGAGAATTCTGTTCCTGACGGTATCACAGTCTCCAGATGTGAACCAGTTGAAGGCCAAGATTGTGCAGTTCATCATGGGGAACCAAGTTCTCAGTCCCAATTCTGTGCTTCCCCAATGGAACTTGCAATACGAGTGGATCACCCCAACGAAAACTCTGGTGGTTTTGGATGATGTGTGGTCACTGGCAGTGCTTGAGCCGCTGCTCTTTAACATCCCTGGCTGCAAGTTTCTTGTGGTCTCACGTTTCAAATTCCCAAACGTTCTCGATGCCACTTATGATGTTGAGTTGTTGAGGGAAGAAGAAGCACTCTCTCTGTTTTGTCACTCTGCTTTTGGCCAAAAGTCGATTCCTCCGGCTGCTGATAAAAATTTGGTCAAGCAGATTGTTAACGAGTGCAAGCGGCTTCCTTTGGCTCTCAAAGTGATTGGAGCTTCATTGAAGGACCAGCCTGAATTGTGTTGGGCAAATGCAAGGAGCAAGTTGTCGAAAGGGGAAGCTTTTTGTGAGTCCCATGAGAACAATCTACTTAAACGTATGGCAACGACTCTTGACTGCTTATCCAAAAAAGTTAAATCATGTTTCTTGGATTTGGGTGCGTTTCCAGAAGACAAGAAGATCCCTCTTGATGTCCTTACCAATATGTGGGTTGAAATACATGGCATCGACGAAGAAGAAGCGTTTGCTATTCTTGTTGAGCTTTCAAACAAAAATCTCCTCACCCTGGTGAAAGATGCAAGAGATGGAGACATATATAGCAGTTGCTATGACATATCTGTTACACAACATGATGTGCTGAGGGACCTTGCTCTTCATTTGAGCAGTGATGGCAGCGTGAACGAACGCAGTCGATTGCTTATGCCAAGAAGAGAGACTGAACTTCCAAGAGAATGGGAGAGGGATTCAGATCAGCCATTTCACGCGAAGATTGTATCAGTTCATACCGGTGAAATGAAAGAGATGGACTGGTTCCAGATGGAGTTTCCCAAATGTGAAGTGCTGATTTTGAATTTTTCATCCGACAACGAGTACCTTTTGCCTCCTTTTATTGAAAACATGCCAAAGTTGAGGGCATTGATAATCATGAATTACAGTACATCAAATGCCATCCTAAATGGCTTTTCAGTCTTCTCAAATTTTCCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009987 GO:0010035 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0023052 GO:0042221 GO:0042742 GO:0043207 GO:0044237 GO:0044464 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0080167 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

622

Amino Acids

70.35

Weight (kDa)

5.92

Isoelectric Point (pI)

46.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	8 - 130	2.9e-10	Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC	PF00931	196 - 354	3.3e-18	NB-ARC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33300 AT4G33300
fragaria_vesca	FvH4_2g40720
malus_domestica	MD08G1041800.v1.1 MD08G1042400.v1.1 MD08G1042500.v1.1
prunus_persica	Prupe.1G389500_v2.0.a1
pyrus_communis	pycom08g03380 pycom08g03410 pycom08g03430 pycom08g03440
rosa_chinensis	RchiOBHm_Chr6g0299881
rosa_laevigata	RLG00000011379
rosa_multiflora	Rmu_ssc0000158.1_g000014
rosa_roxburghii	Rroxscaffold_7G00168310
rosa_rugosa	Rorug06G0293000
rosa_samantha	Rh6AG404300 Rh6BG412500 Rh6CG418500 Rh6DG405400
rosa_wichuraiana	Rw6G035360 Rw6G035380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	20
AatII	GACGTC	1 cut(s)	369
AccB7I	CCANNNNNTGG	2 cut(s)	358, 1362
AccII	CGCG	3 cut(s)	251, 259, 1640
AclI	AACGTT	1 cut(s)	978
AclWI	GGATC	3 cut(s)	399, 863, 1330
AcoI	YGGCCR	3 cut(s)	174, 360, 1054
AcsI	RAATTY	6 cut(s)	636, 723, 968, 1087, 1726, 1852
AcuI	CTGAAG	1 cut(s)	317
AcyI	GRCGYC	2 cut(s)	162, 366
AfaI	GTAC	3 cut(s)	155, 1748, 1813
AfiI	CCNNNNNNNGG	9 cut(s)	205, 232, 346, 358, 379, 430, 769, 1362, 1441
AgeI	ACCGGT	1 cut(s)	1661
AgsI	TTSAA	7 cut(s)	769, 967, 1159, 1370, 1422, 1726, 1769
AhdI	GACNNNNNGTC	1 cut(s)	202
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	2 cut(s)	934, 1440
AjuI	GAANNNNNNNTTGG	4 cut(s)	426, 458, 1050, 1082
AleI	CACNNNNGTG	1 cut(s)	1442
AloI	GAACNNNNNNTCC	2 cut(s)	383, 415
AluBI	AGCT	8 cut(s)	43, 127, 169, 193, 682, 1151, 1217, 1417
AluI	AGCT	8 cut(s)	43, 127, 169, 193, 682, 1151, 1217, 1417
Alw21I	GWGCWC	1 cut(s)	45
Alw26I	GTCTC	4 cut(s)	753, 961, 1458, 1583
AlwI	GGATC	3 cut(s)	399, 863, 1330
AoxI	GGCC	7 cut(s)	174, 223, 234, 337, 360, 771, 1054
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	5 cut(s)	124, 919, 939, 1076, 1548
ApoI	RAATTY	6 cut(s)	636, 723, 968, 1087, 1726, 1852
AsiGI	ACCGGT	1 cut(s)	1661
AspLEI	GCGC	1 cut(s)	261
AspS9I	GGNCC	7 cut(s)	235, 244, 337, 338, 349, 1162, 1519
AsuHPI	GGTGA	6 cut(s)	559, 604, 850, 1429, 1456, 1676
AvaII	GGWCC	4 cut(s)	244, 349, 1162, 1519
BaeGI	GKGCMC	1 cut(s)	341
BalI	TGGCCA	1 cut(s)	1056
BanII	GRGCYC	2 cut(s)	45, 341
BbsI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
Bbv12I	GWGCWC	1 cut(s)	45
BbvCI	CCTCAGC	1 cut(s)	1514
BbvI	GCAGC	5 cut(s)	136, 906, 926, 1063, 1560
BccI	CCATC	8 cut(s)	71, 292, 490, 1454, 1538, 1672, 1687, 1832
BceAI	ACGGC	2 cut(s)	197, 238
BcgI	CGANNNNNNTGC	2 cut(s)	409, 443
BciT130I	CCWGG	2 cut(s)	936, 1442
BclI	TGATCA	1 cut(s)	694
BcoDI	GTCTC	4 cut(s)	753, 961, 1458, 1583
BfaI	CTAG	1 cut(s)	675
BglII	AGATCT	1 cut(s)	704
BisI	GCNGC	8 cut(s)	125, 440, 917, 920, 940, 1077, 1121, 1549
BlsI	GCNGC	8 cut(s)	126, 441, 918, 921, 941, 1078, 1122, 1550
BmcAI	AGTACT	1 cut(s)	155
Bme1390I	CCNGG	2 cut(s)	936, 1442
Bme18I	GGWCC	4 cut(s)	244, 349, 1162, 1519
BmeRI	GACNNNNNGTC	1 cut(s)	202
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	7 cut(s)	235, 244, 337, 338, 349, 1162, 1519
BmiI	GGNNCC	5 cut(s)	94, 339, 800, 1520, 1688
BmrFI	CCNGG	2 cut(s)	936, 1442
BmsI	GCATC	4 cut(s)	417, 976, 1389, 1441
BpiI	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BpmI	CTGGAG	2 cut(s)	200, 735
Bpu10I	CCTNAGC	1 cut(s)	1514
BpuEI	CTTGAG	1 cut(s)	932
BsaBI	GATNNNNATC	1 cut(s)	1623
BsaHI	GRCGYC	2 cut(s)	162, 366
BsaI	GGTCTC	1 cut(s)	961
BsaJI	CCNNGG	3 cut(s)	99, 934, 1440
BsaWI	WCCGGW	2 cut(s)	423, 1661
Bsc4I	CCNNNNNNNGG	9 cut(s)	205, 232, 346, 358, 379, 430, 769, 1362, 1441
Bse118I	RCCGGY	3 cut(s)	172, 405, 1661
Bse1I	ACTGG	3 cut(s)	763, 906, 1688
Bse8I	GATNNNNATC	1 cut(s)	1623
BseBI	CCWGG	2 cut(s)	936, 1442
BseDI	CCNNGG	3 cut(s)	99, 934, 1440
BseGI	GGATG	8 cut(s)	432, 501, 588, 697, 892, 930, 1733, 1824
BseJI	GATNNNNATC	1 cut(s)	1623
BseLI	CCNNNNNNNGG	9 cut(s)	205, 232, 346, 358, 379, 430, 769, 1362, 1441
BseMII	CTCAG	3 cut(s)	38, 823, 1505
BseNI	ACTGG	3 cut(s)	763, 906, 1688
BseRI	GAGGAG	4 cut(s)	515, 526, 536, 1424
BseSI	GKGCMC	1 cut(s)	341
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	5 cut(s)	136, 906, 926, 1063, 1560
BseYI	CCCAGC	1 cut(s)	189
BsgI	GTGCAG	1 cut(s)	803
Bsh1236I	CGCG	3 cut(s)	251, 259, 1640
Bsh1285I	CGRYCG	3 cut(s)	177, 199, 406
BshFI	GGCC	7 cut(s)	176, 225, 236, 339, 362, 773, 1056
BshTI	ACCGGT	1 cut(s)	1661
BsiEI	CGRYCG	3 cut(s)	177, 199, 406
BsiHKAI	GWGCWC	1 cut(s)	45
BsiSI	CCGG	6 cut(s)	159, 173, 406, 424, 1073, 1662
BslFI	GGGAC	6 cut(s)	172, 460, 475, 798, 1213, 1532
BslI	CCNNNNNNNGG	9 cut(s)	205, 232, 346, 358, 379, 430, 769, 1362, 1441
BsmAI	GTCTC	4 cut(s)	753, 961, 1458, 1583
BsmFI	GGGAC	6 cut(s)	172, 460, 475, 798, 1213, 1532
BsnI	GGCC	7 cut(s)	176, 225, 236, 339, 362, 773, 1056
Bso31I	GGTCTC	1 cut(s)	961
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	2 cut(s)	45, 341
Bsp143I	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
BspANI	GGCC	7 cut(s)	176, 225, 236, 339, 362, 773, 1056
BspCNI	CTCAG	3 cut(s)	39, 822, 1506
BspFNI	CGCG	3 cut(s)	251, 259, 1640
BspHI	TCATGA	1 cut(s)	1800
BspLI	GGNNCC	5 cut(s)	94, 339, 800, 1520, 1688
BspPI	GGATC	3 cut(s)	399, 863, 1330
BspQI	GCTCTTC	2 cut(s)	134, 1533
BspTNI	GGTCTC	1 cut(s)	961
BsrFI	RCCGGY	3 cut(s)	172, 405, 1661
BsrI	ACTGG	3 cut(s)	763, 906, 1688
BssAI	RCCGGY	3 cut(s)	172, 405, 1661
BssECI	CCNNGG	3 cut(s)	99, 934, 1440
BssMI	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
BssNI	GRCGYC	2 cut(s)	162, 366
Bst2UI	CCWGG	2 cut(s)	936, 1442
Bst4CI	ACNGT	5 cut(s)	319, 592, 739, 747, 1811
Bst6I	CTCTTC	3 cut(s)	134, 1533, 1579
BstACI	GRCGYC	2 cut(s)	162, 366
BstAPI	GCANNNNNTGC	1 cut(s)	78
BstC8I	GCNNGC	1 cut(s)	1118
BstDEI	CTNAG	4 cut(s)	47, 115, 809, 1514
BstF5I	GGATG	8 cut(s)	432, 501, 588, 697, 892, 930, 1733, 1824
BstFNI	CGCG	3 cut(s)	251, 259, 1640
BstHHI	GCGC	1 cut(s)	261
BstKTI	GATC	7 cut(s)	33, 394, 697, 707, 858, 1338, 1627
BstMAI	GTCTC	4 cut(s)	753, 961, 1458, 1583
BstMBI	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
BstMCI	CGRYCG	3 cut(s)	177, 199, 406
BstMWI	GCNNNNNNNGC	5 cut(s)	78, 190, 436, 916, 1545
BstNI	CCWGG	2 cut(s)	936, 1442
BstNSI	RCATGY	2 cut(s)	205, 1777
BstSCI	CCNGG	2 cut(s)	934, 1440
BstSLI	GKGCMC	1 cut(s)	341
BstUI	CGCG	3 cut(s)	251, 259, 1640
BstV1I	GCAGC	5 cut(s)	136, 906, 926, 1063, 1560
BstV2I	GAAGAC	4 cut(s)	320, 593, 1332, 1837
BstX2I	RGATCY	2 cut(s)	704, 1335
BstXI	CCANNNNNNTGG	1 cut(s)	1608
BstYI	RGATCY	2 cut(s)	704, 1335
BstZI	CGGCCG	1 cut(s)	174
BsuRI	GGCC	7 cut(s)	176, 225, 236, 339, 362, 773, 1056
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	8 cut(s)	432, 501, 588, 697, 892, 930, 1733, 1824
BtsI	GCAGTG	3 cut(s)	12, 912, 1546
BtsIMutI	CAGTG	6 cut(s)	12, 324, 721, 899, 912, 1546
Cac8I	GCNNGC	1 cut(s)	1118
CciI	TCATGA	1 cut(s)	1800
CfoI	GCGC	1 cut(s)	261
Cfr10I	RCCGGY	3 cut(s)	172, 405, 1661
Cfr13I	GGNCC	7 cut(s)	235, 244, 337, 338, 349, 1162, 1519
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	3 cut(s)	154, 1747, 1812
CspAI	ACCGGT	1 cut(s)	1661
CspCI	CAANNNNNGTGG	2 cut(s)	833, 868
CviAII	CATG	9 cut(s)	78, 202, 793, 1232, 1296, 1376, 1505, 1774, 1801
CviQI	GTAC	3 cut(s)	154, 1747, 1812
DdeI	CTNAG	4 cut(s)	47, 115, 809, 1514
DpnI	GATC	7 cut(s)	32, 393, 696, 706, 857, 1337, 1626
DpnII	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
DraI	TTTAAA	1 cut(s)	453
DrdI	GACNNNNNNGTC	1 cut(s)	20
DriI	GACNNNNNGTC	1 cut(s)	202
DseDI	GACNNNNNNGTC	1 cut(s)	20
EaeI	YGGCCR	3 cut(s)	174, 360, 1054
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	3 cut(s)	134, 1533, 1579
Eam1105I	GACNNNNNGTC	1 cut(s)	202
EarI	CTCTTC	3 cut(s)	134, 1533, 1579
EciI	GGCGGA	2 cut(s)	398, 404
Ecl136II	GAGCTC	1 cut(s)	43
EclXI	CGGCCG	1 cut(s)	174
Eco24I	GRGCYC	2 cut(s)	45, 341
Eco31I	GGTCTC	1 cut(s)	961
Eco47I	GGWCC	4 cut(s)	244, 349, 1162, 1519
Eco52I	CGGCCG	1 cut(s)	174
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	1 cut(s)	317
EcoICRI	GAGCTC	1 cut(s)	43
EcoO109I	RGGNCCY	2 cut(s)	337, 1519
EcoRI	GAATTC	1 cut(s)	723
EcoRII	CCWGG	2 cut(s)	934, 1440
EcoT38I	GRGCYC	2 cut(s)	45, 341
FaeI	CATG	9 cut(s)	81, 205, 796, 1235, 1299, 1379, 1508, 1777, 1804
FaqI	GGGAC	6 cut(s)	172, 460, 475, 798, 1213, 1532
FatI	CATG	9 cut(s)	77, 201, 792, 1231, 1295, 1375, 1504, 1773, 1800
FauI	CCCGC	3 cut(s)	77, 467, 482
FbaI	TGATCA	1 cut(s)	694
Fnu4HI	GCNGC	8 cut(s)	125, 440, 917, 920, 940, 1077, 1121, 1549
FokI	GGATG	8 cut(s)	439, 508, 595, 704, 899, 917, 1720, 1811
FriOI	GRGCYC	2 cut(s)	45, 341
Fsp4HI	GCNGC	8 cut(s)	125, 440, 917, 920, 940, 1077, 1121, 1549
FspBI	CTAG	1 cut(s)	675
GlaI	GCGC	1 cut(s)	260
GluI	GCNGC	8 cut(s)	125, 440, 917, 920, 940, 1077, 1121, 1549
GsaI	CCCAGC	1 cut(s)	193
GsuI	CTGGAG	2 cut(s)	200, 735
HaeIII	GGCC	7 cut(s)	176, 225, 236, 339, 362, 773, 1056
HapII	CCGG	6 cut(s)	159, 173, 406, 424, 1073, 1662
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	1 cut(s)	261
Hin1I	GRCGYC	2 cut(s)	162, 366
Hin1II	CATG	9 cut(s)	81, 205, 796, 1235, 1299, 1379, 1508, 1777, 1804
Hin6I	GCGC	1 cut(s)	259
HinP1I	GCGC	1 cut(s)	259
HincII	GTYRAC	1 cut(s)	1108
HindII	GTYRAC	1 cut(s)	1108
HindIII	AAGCTT	1 cut(s)	1215
HinfI	GANTC	4 cut(s)	1066, 1226, 1264, 1619
HpaI	GTTAAC	1 cut(s)	1108
HpaII	CCGG	6 cut(s)	159, 173, 406, 424, 1073, 1662
HphI	GGTGA	6 cut(s)	559, 604, 850, 1429, 1456, 1676
Hpy166II	GTNNAC	5 cut(s)	247, 370, 760, 1108, 1555
Hpy188I	TCNGA	5 cut(s)	26, 208, 284, 1624, 1738
Hpy8I	GTNNAC	5 cut(s)	247, 370, 760, 1108, 1555
Hpy99I	CGWCG	4 cut(s)	26, 167, 368, 1388
HpyAV	CCTTC	4 cut(s)	521, 589, 763, 1153
HpyCH4III	ACNGT	5 cut(s)	319, 592, 739, 747, 1811
HpyCH4IV	ACGT	5 cut(s)	279, 366, 961, 978, 1252
HpyCH4V	TGCA	7 cut(s)	687, 784, 844, 942, 1116, 1190, 1454
HpyF10VI	GCNNNNNNNGC	5 cut(s)	78, 190, 436, 916, 1545
HpyF3I	CTNAG	4 cut(s)	47, 115, 809, 1514
HpySE526I	ACGT	5 cut(s)	279, 366, 961, 978, 1252
Hsp92I	GRCGYC	2 cut(s)	162, 366
Hsp92II	CATG	9 cut(s)	81, 205, 796, 1235, 1299, 1379, 1508, 1777, 1804
HspAI	GCGC	1 cut(s)	259
Ksp22I	TGATCA	1 cut(s)	694
KspAI	GTTAAC	1 cut(s)	1108
Kzo9I	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
LguI	GCTCTTC	2 cut(s)	134, 1533
LmnI	GCTCC	4 cut(s)	40, 48, 1148, 1194
Lsp1109I	GCAGC	5 cut(s)	136, 906, 926, 1063, 1560
LweI	GCATC	4 cut(s)	417, 976, 1389, 1441
MaeI	CTAG	1 cut(s)	675
MaeII	ACGT	5 cut(s)	279, 366, 961, 978, 1252
MaeIII	GTNAC	4 cut(s)	7, 897, 1040, 1495
MalI	GATC	7 cut(s)	32, 393, 696, 706, 857, 1337, 1626
MboI	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
MflI	RGATCY	2 cut(s)	704, 1335
MhlI	GDGCHC	2 cut(s)	45, 341
MlsI	TGGCCA	1 cut(s)	1056
MluNI	TGGCCA	1 cut(s)	1056
MlyI	GAGTC	2 cut(s)	1235, 1258
MmeI	TCCRAC	4 cut(s)	4, 186, 613, 1761
Mox20I	TGGCCA	1 cut(s)	1056
MscI	TGGCCA	1 cut(s)	1056
MseI	TTAA	6 cut(s)	56, 452, 927, 1107, 1248, 1290
MslI	CAYNNNNRTG	2 cut(s)	1442, 1662
Msp20I	TGGCCA	1 cut(s)	1056
MspA1I	CMGCKG	3 cut(s)	397, 439, 919
MspI	CCGG	6 cut(s)	159, 173, 406, 424, 1073, 1662
MspR9I	CCNGG	2 cut(s)	936, 1442
MvaI	CCWGG	2 cut(s)	936, 1442
MvnI	CGCG	3 cut(s)	251, 259, 1640
MwoI	GCNNNNNNNGC	5 cut(s)	78, 190, 436, 916, 1545
NdeII	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
NlaIII	CATG	9 cut(s)	81, 205, 796, 1235, 1299, 1379, 1508, 1777, 1804
NlaIV	GGNNCC	5 cut(s)	94, 339, 800, 1520, 1688
NmeAIII	GCCGAG	1 cut(s)	124
NmuCI	GTSAC	3 cut(s)	7, 897, 1040
NspI	RCATGY	2 cut(s)	205, 1777
OliI	CACNNNNGTG	1 cut(s)	1442
PagI	TCATGA	1 cut(s)	1800
PciSI	GCTCTTC	2 cut(s)	134, 1533
PfeI	GAWTC	2 cut(s)	1066, 1619
PflFI	GACNNNGTC	1 cut(s)	319
PflMI	CCANNNNNTGG	2 cut(s)	358, 1362
PinAI	ACCGGT	1 cut(s)	1661
PkrI	GCNGC	8 cut(s)	126, 441, 918, 921, 941, 1078, 1122, 1550
PleI	GAGTC	2 cut(s)	1234, 1258
PpsI	GAGTC	2 cut(s)	1234, 1258
PpuMI	RGGWCCY	1 cut(s)	1519
Psp124BI	GAGCTC	1 cut(s)	45
Psp1406I	AACGTT	1 cut(s)	978
Psp5II	RGGWCCY	1 cut(s)	1519
Psp6I	CCWGG	2 cut(s)	934, 1440
PspFI	CCCAGC	1 cut(s)	189
PspGI	CCWGG	2 cut(s)	934, 1440
PspN4I	GGNNCC	5 cut(s)	94, 339, 800, 1520, 1688
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	7 cut(s)	235, 244, 337, 338, 349, 1162, 1519
PspPPI	RGGWCCY	1 cut(s)	1519
PsrI	GAACNNNNNNTAC	2 cut(s)	830, 862
PsuI	RGATCY	2 cut(s)	704, 1335
PsyI	GACNNNGTC	1 cut(s)	319
RsaI	GTAC	3 cut(s)	155, 1748, 1813
RsaNI	GTAC	3 cut(s)	154, 1747, 1812
RseI	CAYNNNNRTG	2 cut(s)	1442, 1662
SacI	GAGCTC	1 cut(s)	45
SapI	GCTCTTC	2 cut(s)	134, 1533
SaqAI	TTAA	6 cut(s)	56, 452, 927, 1107, 1248, 1290
SatI	GCNGC	8 cut(s)	125, 440, 917, 920, 940, 1077, 1121, 1549
Sau3AI	GATC	7 cut(s)	30, 391, 694, 704, 855, 1335, 1624
Sau96I	GGNCC	7 cut(s)	235, 244, 337, 338, 349, 1162, 1519
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	2 cut(s)	1235, 1258
ScrFI	CCNGG	2 cut(s)	936, 1442
SduI	GDGCHC	2 cut(s)	45, 341
SfaNI	GCATC	4 cut(s)	417, 976, 1389, 1441
SinI	GGWCC	4 cut(s)	244, 349, 1162, 1519
SmiMI	CAYNNNNRTG	2 cut(s)	1442, 1662
SmlI	CTYRAG	1 cut(s)	911
SmoI	CTYRAG	1 cut(s)	911
SspMI	CTAG	1 cut(s)	675
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	2 cut(s)	934, 1440
TaaI	ACNGT	5 cut(s)	319, 592, 739, 747, 1811
TaiI	ACGT	5 cut(s)	282, 369, 964, 981, 1255
TatI	WGTACW	2 cut(s)	153, 1811
TauI	GCSGC	3 cut(s)	442, 919, 1123
TfiI	GAWTC	2 cut(s)	1066, 1619
Tru1I	TTAA	6 cut(s)	56, 452, 927, 1107, 1248, 1290
Tru9I	TTAA	6 cut(s)	56, 452, 927, 1107, 1248, 1290
TscAI	CASTG	6 cut(s)	12, 324, 721, 906, 912, 1546
TseFI	GTSAC	3 cut(s)	7, 897, 1040
TseI	GCWGC	5 cut(s)	124, 919, 939, 1076, 1548
Tsp45I	GTSAC	3 cut(s)	7, 897, 1040
TspDTI	ATGAA	9 cut(s)	551, 599, 778, 1143, 1520, 1646, 1685, 1722, 1817
TspGWI	ACGGA	2 cut(s)	26, 53
TspRI	CASTG	6 cut(s)	12, 324, 721, 906, 912, 1546
Tth111I	GACNNNGTC	1 cut(s)	319
Van91I	CCANNNNNTGG	2 cut(s)	358, 1362
VpaK11BI	GGWCC	4 cut(s)	244, 349, 1162, 1519
XapI	RAATTY	6 cut(s)	636, 723, 968, 1087, 1726, 1852
XceI	RCATGY	2 cut(s)	205, 1777
XspI	CTAG	1 cut(s)	675
ZraI	GACGTC	1 cut(s)	367
ZrmI	AGTACT	1 cut(s)	155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.