FvH4_3g00330
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013499
Physical Location & Seq
Reverse (-)
193336 .. 196048
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00330.t1

Sequence Viewer

Length: 1419 bp
ATGGAGGTGCTGACCGGCGACCGGAATATGCCGGACCCAGCAGCAGCAGAAGGTGAGGAGTTCCCGGCGCACATAACCCCATTATTCACTGAGCCACACCAACAACTTGTCTTTGACAATCCAACGGCGGCTGCTATGTCGCCACAGAAGCTCCGTATCAGGTGCAACGGCAGGTCTGCTCTACCAGATGATGCTGATCTCGCCTTACTCGACAAAGGCTTGTCACCACGTAATGATGTTGATGAGGCACACGGTGTACAATTCGTGAATTGGAAGCGGAAGAGAAAGAGAACATCGTCTTGTGCTAGTTCTACAAGAAAGAGCAGAAGGCTTGAAAAGCTCGAGCATTTTCTGGAAAGTCTGGTAAACAAAATGCTGGAGAAGCAGGAACAGATGCATATGAAGTTGATAGAAATGGTACAAGACAAGGAGAAAGAGAGAATAGCCAAAGAAGAAGCCTGGAAACAGCAGGAGTTGGAGAGGATCATAAGGGATGAGGAGGTAAGGGCGCAAGAGACATCACGCAGCCTTGCCATCGTTAACTTTATACAAAATCTTTTGGGCAGTGATGTTCAACTCCCCCAACCAGCTCCAATTGTAGCACAACCAGTAGCAGCTGTACCTTCTAGTTCCCATCATGACCATCAATGTGTGGAAGAAAATGATAAGCTTCACAAAGACTGTCATGTTCAAGTTCCCCAACCAGCTCCACTTGTAGCACAATCAGTAGCAACAGTACCTCCTACTTCTAATGATGACCATCAATGTGCGGAACAAAATGATAATCTTCACAAAGACACGGCCACTGTCAAGTGTAGTGCAACAGCAACTGATAAAAGATGGATCCAAGCTGAAGCGCAGGCACTTATAGGGCTGTGGGCTAGTGTGGAGCATAAGTTCCGCACCCCATCAAATTCTGGCCCTGGAGCTCTATGGGAGGAGATAGCTCTTGGGATGAACAGTATGGGCTTTGATCGCACAGGGAAGAAGTGTAAAGAGAAATGGGAAAACATGAACAAGTACTTCAAAAAGGCAATCACAAATGACAGAAAGCGTTCTGCGAATGACAAGACTTGTCAATATTTTAATGAATTGGAGTTGCTCTACAAAAGTGGACTTCTGAATCTGGGGAATGTCGGCTTTAGTCCCCCAAGCAATCTGGAGAATGGCGGGTTTAGTCCCACAAGCAATCTGGGGAATGGCAGTTGTAGTCCCACAAGCAATATGGGGAATGGCAGCCTTAGTCCCACAAGCAATATGGGGAATGGCGGCTTTAGTCCTACAAACAACCAGAAGAGTGGAAACGAGTCCCAACAGTGCGTGCCCAGTGACACTAGTCTTCTAACTCGTGCCACAATACCGTTTCAGATCGATGAAGCTTCAGAGAGAACAAGAGTAGTGGTTTCTCCTGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_4

Protein Analysis

473

Amino Acids

52.26

Weight (kDa)

6.15

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 279 - 367 1.1e-20 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47660
fragaria_vesca FvH4_3g00330
malus_domestica MD05G1361500.v1.1 MD10G1338800.v1.1
prunus_persica Prupe.4G003900_v2.0.a1
pyrus_communis pycom05g32800 pycom10g28850
rosa_chinensis RchiOBHm_Chr5g0000511
rosa_laevigata RLG00000030873
rosa_multiflora Rmu_co7961544.1_g000001
rosa_roxburghii Rroxscaffold_1G00075670
rosa_rugosa Rorug04G0384300
rosa_samantha Rh5AG004200 Rh5BG005700 Rh5CG004600 Rh5DG004500 Rh5DG004600
rosa_wichuraiana Rw5G000430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AciI CCGC 6 cut(s) 128, 277, 770, 901, 1170, 1269
AclWI GGATC 3 cut(s) 491, 838, 851
AcoI YGGCCR 1 cut(s) 801
AcsI RAATTY 1 cut(s) 913
AcuI CTGAAG 2 cut(s) 873, 1365
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 5 cut(s) 258, 420, 621, 738, 1022
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 4 cut(s) 335, 575, 692, 1027
AhlI ACTAGT 1 cut(s) 1334
AjnI CCWGG 2 cut(s) 458, 922
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 1 cut(s) 509
AlwI GGATC 3 cut(s) 491, 838, 851
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 2 cut(s) 801, 919
ApeKI GCWGC 6 cut(s) 41, 44, 131, 525, 614, 1236
ApoI RAATTY 1 cut(s) 913
Asp700I GAANNNNTTC 1 cut(s) 1054
AspLEI GCGC 3 cut(s) 70, 511, 859
AspS9I GGNCC 2 cut(s) 34, 920
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 65, 216
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 1326
BamHI GGATCC 1 cut(s) 843
BanII GRGCYC 1 cut(s) 931
BauI CACGAG 1 cut(s) 1347
BbsI GAAGAC 1 cut(s) 1331
Bbv12I GWGCWC 1 cut(s) 931
BbvI GCAGC 6 cut(s) 53, 56, 118, 537, 626, 1248
BccI CCATC 6 cut(s) 542, 642, 651, 768, 834, 916
BceAI ACGGC 3 cut(s) 141, 184, 816
BciT130I CCWGG 2 cut(s) 460, 924
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 509
BcuI ACTAGT 1 cut(s) 1334
BfaI CTAG 4 cut(s) 306, 627, 882, 1335
BfuAI ACCTGC 1 cut(s) 162
BisI GCNGC 8 cut(s) 42, 45, 129, 132, 526, 615, 1237, 1270
BlsI GCNGC 8 cut(s) 43, 46, 130, 133, 527, 616, 1238, 1271
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 3 cut(s) 65, 460, 924
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 34, 920
BmiI GGNNCC 2 cut(s) 36, 845
BmrFI CCNGG 3 cut(s) 65, 460, 924
BmrI ACTGGG 1 cut(s) 1320
BmsI GCATC 2 cut(s) 181, 384
BmuI ACTGGG 1 cut(s) 1320
BoxI GACNNNNGTC 1 cut(s) 1335
BpiI GAAGAC 1 cut(s) 1331
BpmI CTGGAG 3 cut(s) 398, 945, 1181
BpuMI CCSGG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 1371
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 2 cut(s) 195, 759
BsaJI CCNNGG 1 cut(s) 922
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 6 cut(s) 135, 165, 724, 754, 881, 911
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 608, 1326
Bse8I GATNNNNATC 2 cut(s) 195, 759
BseBI CCWGG 2 cut(s) 460, 924
BseCI ATCGAT 1 cut(s) 1371
BseDI CCNNGG 1 cut(s) 922
BseGI GGATG 2 cut(s) 499, 960
BseJI GATNNNNATC 2 cut(s) 195, 759
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 608, 1326
BseRI GAGGAG 3 cut(s) 71, 512, 953
BseSI GKGCMC 1 cut(s) 1326
BseXI GCAGC 6 cut(s) 53, 56, 118, 537, 626, 1248
BseYI CCCAGC 1 cut(s) 37
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 803, 921
BshVI ATCGAT 1 cut(s) 1371
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 931
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 4 cut(s) 15, 22, 32, 65
BslFI GGGAC 5 cut(s) 1131, 1164, 1197, 1230, 1294
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 1 cut(s) 509
BsmFI GGGAC 5 cut(s) 1131, 1164, 1197, 1230, 1294
BsnI GGCC 2 cut(s) 803, 921
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 931, 1326
Bsp1407I TGTACA 1 cut(s) 256
Bsp143I GATC 5 cut(s) 196, 483, 843, 973, 1368
BspACI CCGC 6 cut(s) 128, 277, 770, 901, 1170, 1269
BspANI GGCC 2 cut(s) 803, 921
BspCNI CTCAG 1 cut(s) 82
BspDI ATCGAT 1 cut(s) 1371
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 36, 845
BspMI ACCTGC 1 cut(s) 162
BspPI GGATC 3 cut(s) 491, 838, 851
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 256
BsrI ACTGG 2 cut(s) 608, 1326
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 922
BssMI GATC 5 cut(s) 196, 483, 843, 973, 1368
BssSI CACGAG 1 cut(s) 1347
Bst2BI CACGAG 1 cut(s) 1347
Bst2UI CCWGG 2 cut(s) 460, 924
Bst4CI ACNGT 7 cut(s) 254, 683, 736, 808, 962, 1317, 1362
Bst6I CTCTTC 2 cut(s) 275, 1289
BstAUI TGTACA 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 861, 1322
BstDEI CTNAG 2 cut(s) 90, 1241
BstF5I GGATG 2 cut(s) 499, 960
BstHHI GCGC 3 cut(s) 70, 511, 859
BstKTI GATC 5 cut(s) 199, 486, 846, 976, 1371
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 5 cut(s) 196, 483, 843, 973, 1368
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 5 cut(s) 148, 200, 337, 382, 975
BstNI CCWGG 2 cut(s) 460, 924
BstPAI GACNNNNGTC 1 cut(s) 1335
BstSCI CCNGG 3 cut(s) 63, 458, 922
BstSLI GKGCMC 1 cut(s) 1326
BstV1I GCAGC 6 cut(s) 53, 56, 118, 537, 626, 1248
BstV2I GAAGAC 1 cut(s) 1331
BstX2I RGATCY 1 cut(s) 843
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 1 cut(s) 843
Bsu15I ATCGAT 1 cut(s) 1371
BsuRI GGCC 2 cut(s) 803, 921
BsuTUI ATCGAT 1 cut(s) 1371
BtsCI GGATG 2 cut(s) 499, 960
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 5 cut(s) 87, 571, 804, 1322, 1333
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 2 cut(s) 861, 1322
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 1 cut(s) 637
CfoI GCGC 3 cut(s) 70, 511, 859
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 34, 920
ClaI ATCGAT 1 cut(s) 1371
Csp6I GTAC 5 cut(s) 257, 419, 620, 737, 1021
CspCI CAANNNNNGTGG 2 cut(s) 1380, 1415
CviAII CATG 3 cut(s) 638, 686, 1012
CviQI GTAC 5 cut(s) 257, 419, 620, 737, 1021
DdeI CTNAG 2 cut(s) 90, 1241
DpnI GATC 5 cut(s) 198, 485, 845, 975, 1370
DpnII GATC 5 cut(s) 196, 483, 843, 973, 1368
DraIII CACNNNGTG 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 801
Eam1104I CTCTTC 2 cut(s) 275, 1289
EarI CTCTTC 2 cut(s) 275, 1289
Ecl136II GAGCTC 1 cut(s) 929
Eco24I GRGCYC 1 cut(s) 931
Eco47I GGWCC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 929
Eco57I CTGAAG 2 cut(s) 873, 1365
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 929
EcoRII CCWGG 2 cut(s) 458, 922
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 931
FaeI CATG 3 cut(s) 641, 689, 1015
FaqI GGGAC 5 cut(s) 1131, 1164, 1197, 1230, 1294
FatI CATG 3 cut(s) 637, 685, 1011
FauI CCCGC 1 cut(s) 1163
FauNDI CATATG 1 cut(s) 399
Fnu4HI GCNGC 8 cut(s) 42, 45, 129, 132, 526, 615, 1237, 1270
FokI GGATG 2 cut(s) 506, 967
FriOI GRGCYC 1 cut(s) 931
Fsp4HI GCNGC 8 cut(s) 42, 45, 129, 132, 526, 615, 1237, 1270
FspBI CTAG 4 cut(s) 306, 627, 882, 1335
GlaI GCGC 3 cut(s) 69, 510, 858
GluI GCNGC 8 cut(s) 42, 45, 129, 132, 526, 615, 1237, 1270
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 3 cut(s) 398, 945, 1181
HaeIII GGCC 2 cut(s) 803, 921
HapII CCGG 4 cut(s) 15, 22, 32, 65
HhaI GCGC 3 cut(s) 70, 511, 859
Hin1II CATG 3 cut(s) 641, 689, 1015
Hin6I GCGC 3 cut(s) 68, 509, 857
HinP1I GCGC 3 cut(s) 68, 509, 857
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 2 cut(s) 668, 1377
HinfI GANTC 2 cut(s) 1123, 1307
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 4 cut(s) 15, 22, 32, 65
HphI GGTGA 2 cut(s) 65, 216
Hpy166II GTNNAC 4 cut(s) 257, 367, 541, 1115
Hpy188I TCNGA 3 cut(s) 1122, 1368, 1384
Hpy188III TCNNGA 4 cut(s) 265, 353, 638, 1160
Hpy8I GTNNAC 4 cut(s) 257, 367, 541, 1115
HpyAV CCTTC 3 cut(s) 44, 321, 633
HpyCH4III ACNGT 7 cut(s) 254, 683, 736, 808, 962, 1317, 1362
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 165, 397, 821
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 200, 337, 382, 975
HpyF3I CTNAG 2 cut(s) 90, 1241
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 3 cut(s) 641, 689, 1015
HspAI GCGC 3 cut(s) 68, 509, 857
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 5 cut(s) 196, 483, 843, 973, 1368
LmnI GCTCC 5 cut(s) 156, 595, 712, 889, 926
Lsp1109I GCAGC 6 cut(s) 53, 56, 118, 537, 626, 1248
LweI GCATC 2 cut(s) 181, 384
MaeI CTAG 4 cut(s) 306, 627, 882, 1335
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 2 cut(s) 222, 1328
MalI GATC 5 cut(s) 198, 485, 845, 975, 1370
MboI GATC 5 cut(s) 196, 483, 843, 973, 1368
MboII GAAGA 7 cut(s) 292, 464, 668, 779, 997, 1306, 1331
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 1 cut(s) 843
MhlI GDGCHC 2 cut(s) 931, 1326
MluCI AATT 5 cut(s) 260, 268, 594, 913, 1091
MlyI GAGTC 1 cut(s) 1316
MmeI TCCRAC 2 cut(s) 146, 456
MnlI CCTC 7 cut(s) 49, 238, 474, 490, 493, 750, 931
Mph1103I ATGCAT 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 3 cut(s) 540, 1086, 1417
MslI CAYNNNNRTG 2 cut(s) 648, 765
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 4 cut(s) 15, 22, 32, 65
MspR9I CCNGG 3 cut(s) 65, 460, 924
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 2 cut(s) 460, 924
MwoI GCNNNNNNNGC 5 cut(s) 148, 200, 337, 382, 975
NciI CCSGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 399
NdeII GATC 5 cut(s) 196, 483, 843, 973, 1368
NlaIII CATG 3 cut(s) 641, 689, 1015
NlaIV GGNNCC 2 cut(s) 36, 845
NmuCI GTSAC 2 cut(s) 222, 1328
NsiI ATGCAT 1 cut(s) 399
PaeR7I CTCGAG 1 cut(s) 341
PagI TCATGA 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 1 cut(s) 1123
PkrI GCNGC 8 cut(s) 43, 46, 130, 133, 527, 616, 1238, 1271
PleI GAGTC 1 cut(s) 1315
PpsI GAGTC 1 cut(s) 1315
Ppu21I YACGTR 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 1335
Psp124BI GAGCTC 1 cut(s) 931
Psp6I CCWGG 2 cut(s) 458, 922
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 458, 922
PspN4I GGNNCC 2 cut(s) 36, 845
PspPI GGNCC 2 cut(s) 34, 920
PspXI VCTCGAGB 1 cut(s) 341
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 843
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 5 cut(s) 258, 420, 621, 738, 1022
RsaNI GTAC 5 cut(s) 257, 419, 620, 737, 1021
RseI CAYNNNNRTG 2 cut(s) 648, 765
SacI GAGCTC 1 cut(s) 931
SaqAI TTAA 3 cut(s) 540, 1086, 1417
SatI GCNGC 8 cut(s) 42, 45, 129, 132, 526, 615, 1237, 1270
Sau3AI GATC 5 cut(s) 196, 483, 843, 973, 1368
Sau96I GGNCC 2 cut(s) 34, 920
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 1 cut(s) 1316
ScrFI CCNGG 3 cut(s) 65, 460, 924
SduI GDGCHC 2 cut(s) 931, 1326
SfaNI GCATC 2 cut(s) 181, 384
Sfr274I CTCGAG 1 cut(s) 341
SinI GGWCC 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 2 cut(s) 648, 765
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 1334
Sse9I AATT 5 cut(s) 260, 268, 594, 913, 1091
SsiI CCGC 6 cut(s) 128, 277, 770, 901, 1170, 1269
SspI AATATT 1 cut(s) 1082
SspMI CTAG 4 cut(s) 306, 627, 882, 1335
SstI GAGCTC 1 cut(s) 931
StyD4I CCNGG 3 cut(s) 63, 458, 922
TaaI ACNGT 7 cut(s) 254, 683, 736, 808, 962, 1317, 1362
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 210, 342, 1371
TasI AATT 5 cut(s) 260, 268, 594, 913, 1091
TatI WGTACW 2 cut(s) 256, 1020
TauI GCSGC 2 cut(s) 131, 1272
TfiI GAWTC 1 cut(s) 1123
Tru1I TTAA 3 cut(s) 540, 1086, 1417
Tru9I TTAA 3 cut(s) 540, 1086, 1417
TscAI CASTG 5 cut(s) 94, 571, 811, 1322, 1333
TseFI GTSAC 2 cut(s) 222, 1328
TseI GCWGC 6 cut(s) 41, 44, 131, 525, 614, 1236
Tsp45I GTSAC 2 cut(s) 222, 1328
TspDTI ATGAA 5 cut(s) 416, 971, 1028, 1104, 1389
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 5 cut(s) 94, 571, 811, 1322, 1333
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 3 cut(s) 1189, 1222, 1255
XhoI CTCGAG 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 4 cut(s) 306, 627, 882, 1335
ZrmI AGTACT 1 cut(s) 1022
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.