FvH4_3g02852

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010536
Physical Location & Seq
Forward (+)
1528115 .. 1528692
578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02852.t1

Sequence Viewer

Length: 489 bp
ATGCAACACGTGTCCAAAACAGACTTCGCCCAGGCCTCGCTCACCGCCCCGCATCATATCACTGTAGCGTCGTACGCGAACTCGAGCACCAAGCGATCCTATCCCGCGGGTACTACAGTCCATTTCCAACTCTACCTCGCCATGCACTGTAGAATCCTACACGAACTAGAACAAAGGATCCCGCGAGGACATCGAATCCCGCGAGGTATCAGAATCCTTGCACAATTCCAACTCGCGTGTTGCCAGACTTCGCCACCCTTCGTGGGCTTCGCATTAAAAAAGGGCCACAGTGCTATTATTCTAGATGTTAAATCCTCCTTTACTACTACTAATAACTTCCAGTTGCAATTTGCTCATAGACAAGCCAATATGGTGGCACATAGGTTAGCAGGTCTTGGTCTACATTCCAATATTCATTGTGACTGGTTCTCTATTGCTCCAGAACTCATTCTGGATGCCATGATGTACAATCAGAATCGTATTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

163

Amino Acids

18.28

Weight (kDa)

9.8

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 400
AccII CGCG 5 cut(s) 77, 107, 184, 202, 236
AciI CCGC 6 cut(s) 45, 50, 105, 107, 182, 200
AclWI GGATC 3 cut(s) 90, 172, 185
AcvI CACGTG 1 cut(s) 10
AfaI GTAC 3 cut(s) 74, 112, 467
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 2 cut(s) 7, 9
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
Alw21I GWGCWC 1 cut(s) 89
AlwI GGATC 3 cut(s) 90, 172, 185
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 2 cut(s) 33, 283
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 82
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BamHI GGATCC 1 cut(s) 177
BbrPI CACGTG 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 32
BfaI CTAG 2 cut(s) 167, 302
BfmI CTRYAG 3 cut(s) 63, 114, 148
BfuAI ACCTGC 1 cut(s) 380
Bme1390I CCNGG 1 cut(s) 32
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 2 cut(s) 61, 445
BpmI CTGGAG 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 30, 105
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 340, 428
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 30, 105
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 1 cut(s) 263
BseNI ACTGG 2 cut(s) 340, 428
Bsh1236I CGCG 5 cut(s) 77, 107, 184, 202, 236
BshFI GGCC 2 cut(s) 35, 285
BsiHKAI GWGCWC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 82
BsiWI CGTACG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 263
BsnI GGCC 2 cut(s) 35, 285
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 89
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 2 cut(s) 95, 177
BspACI CCGC 6 cut(s) 45, 50, 105, 107, 182, 200
BspANI GGCC 2 cut(s) 35, 285
BspFNI CGCG 5 cut(s) 77, 107, 184, 202, 236
BspLI GGNNCC 1 cut(s) 179
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 90, 172, 185
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 2 cut(s) 340, 428
BssECI CCNNGG 2 cut(s) 30, 105
BssMI GATC 2 cut(s) 95, 177
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 4 cut(s) 64, 118, 149, 290
BstAUI TGTACA 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 5 cut(s) 77, 107, 184, 202, 236
BstKTI GATC 2 cut(s) 98, 180
BstMBI GATC 2 cut(s) 95, 177
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 3 cut(s) 63, 114, 148
BstUI CGCG 5 cut(s) 77, 107, 184, 202, 236
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 2 cut(s) 35, 285
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 3 cut(s) 60, 145, 295
BveI ACCTGC 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 283
Cfr42I CCGCGG 1 cut(s) 108
CseI GACGC 1 cut(s) 57
Csp6I GTAC 3 cut(s) 73, 111, 466
CviAII CATG 2 cut(s) 142, 460
CviJI RGCY 4 cut(s) 35, 267, 285, 365
CviKI_1 RGCY 4 cut(s) 35, 267, 285, 365
CviQI GTAC 3 cut(s) 73, 111, 466
DpnI GATC 2 cut(s) 97, 179
DpnII GATC 2 cut(s) 95, 177
Eco147I AGGCCT 1 cut(s) 35
Eco72I CACGTG 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 145, 463
FaiI YATR 6 cut(s) 57, 143, 357, 371, 381, 461
FatI CATG 2 cut(s) 141, 459
FauI CCCGC 5 cut(s) 57, 100, 112, 189, 207
FblI GTMKAC 1 cut(s) 400
FokI GGATG 1 cut(s) 467
FspBI CTAG 2 cut(s) 167, 302
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 2 cut(s) 35, 285
HgaI GACGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 145, 463
HinfI GANTC 4 cut(s) 153, 195, 213, 475
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 2 cut(s) 212, 474
Hpy188III TCNNGA 3 cut(s) 302, 440, 452
Hpy8I GTNNAC 1 cut(s) 401
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 4 cut(s) 64, 118, 149, 290
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 4 cut(s) 4, 145, 221, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 2 cut(s) 145, 463
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 2 cut(s) 95, 177
LmnI GCTCC 1 cut(s) 442
LpnPI CCDG 8 cut(s) 17, 44, 257, 353, 375, 409, 437, 453
LweI GCATC 2 cut(s) 61, 445
MaeI CTAG 2 cut(s) 167, 302
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 419
MalI GATC 2 cut(s) 97, 179
MboI GATC 2 cut(s) 95, 177
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 2 cut(s) 224, 347
MmeI TCCRAC 2 cut(s) 151, 253
MnlI CCTC 5 cut(s) 46, 146, 179, 197, 325
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 2 cut(s) 275, 309
MspA1I CMGCKG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 5 cut(s) 77, 107, 184, 202, 236
MwoI GCNNNNNNNGC 1 cut(s) 74
NdeII GATC 2 cut(s) 95, 177
NlaIII CATG 2 cut(s) 145, 463
NlaIV GGNNCC 1 cut(s) 179
NmuCI GTSAC 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 82
PceI AGGCCT 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 4 cut(s) 153, 195, 213, 475
Pfl23II CGTACG 1 cut(s) 72
PmaCI CACGTG 1 cut(s) 10
PmlI CACGTG 1 cut(s) 10
Ppu21I YACGTR 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 30
PspCI CACGTG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 30
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 283
PspXI VCTCGAGB 1 cut(s) 82
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 3 cut(s) 74, 112, 467
RsaNI GTAC 3 cut(s) 73, 111, 466
SacII CCGCGG 1 cut(s) 108
SaqAI TTAA 2 cut(s) 275, 309
Sau3AI GATC 2 cut(s) 95, 177
Sau96I GGNCC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 89
SetI ASST 5 cut(s) 12, 138, 208, 386, 394
SfaNI GCATC 2 cut(s) 61, 445
SfcI CTRYAG 3 cut(s) 63, 114, 148
Sfr274I CTCGAG 1 cut(s) 82
Sfr303I CCGCGG 1 cut(s) 108
SgrBI CCGCGG 1 cut(s) 108
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 2 cut(s) 224, 347
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 6 cut(s) 45, 50, 105, 107, 182, 200
SspI AATATT 1 cut(s) 412
SspMI CTAG 2 cut(s) 167, 302
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 4 cut(s) 64, 118, 149, 290
TaiI ACGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 83, 193
TasI AATT 2 cut(s) 224, 347
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 4 cut(s) 153, 195, 213, 475
Tru1I TTAA 2 cut(s) 275, 309
Tru9I TTAA 2 cut(s) 275, 309
TscAI CASTG 3 cut(s) 67, 152, 295
TseFI GTSAC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 404
TspRI CASTG 3 cut(s) 67, 152, 295
XbaI TCTAGA 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 167, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.