RchiOBHm_Chr2g0122921

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010536
Physical Location & Seq
Reverse (-)
36078092 .. 36078866
775 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49528

Sequence Viewer

Length: 573 bp
ATGGCTTGGCGTGAGGTATATCTACAGGCTACTTGTTCTTCAGCTACTACAAAATGGGCCCGGGCAAAACCTAGTAAGGTGTGGGCTACGCCGGAGGAAGGTAGCTTGAAGCTTAATGTGGACGGGGCGTTCCTGGCCTCTATTCAGTATGGTGGTACGCGAGGGGTACTTAGAAACTCTCAGGGTCAGTTCCTAGCTGGTTTTTCCTTCAGAGTGGATTTTGTCACCTACCCACTGCATGTCGAACTCCTTACATTGAAAAATGGCCTAGAATTGTTGCAAGCCATGCAGATTACTCATGCCCCCGTCGAGAGTGATTGTCTAATTGTGGTGCAGGCTATTGCTTCTATAACCCCTAACTTGTCTCCCTTAAGTGCATTAATCACAGATATTCAAGGCCTTCTAGCTGGAAGTGAGGACTTAAAGCTTCTCTATGTTCCTCGTCAAGCCAATACATTAGCACATAGGTTGGCTAACTTCAACTTTGATTCCAATGTCCATCTTGACTGGTTTGTTAATGCTCCGGAATTTACCCTTGATGCCCTAATGTATGATTTCAGTCGTATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

190

Amino Acids

21.04

Weight (kDa)

6.06

Isoelectric Point (pI)

24.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 39 - 160 2.2e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 160
AccIII TCCGGA 1 cut(s) 523
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 24, 193
AfaI GTAC 2 cut(s) 157, 168
AfiI CCNNNNNNNGG 1 cut(s) 98
AflII CTTAAG 1 cut(s) 370
AgsI TTSAA 4 cut(s) 109, 259, 395, 481
AjnI CCWGG 1 cut(s) 132
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 6 cut(s) 44, 105, 112, 197, 407, 427
AluI AGCT 6 cut(s) 44, 105, 112, 197, 407, 427
Alw26I GTCTC 1 cut(s) 369
Ama87I CYCGRG 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 4 cut(s) 57, 135, 265, 397
ApaI GGGCCC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 527
AseI ATTAAT 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 57, 58
AsuC2I CCSGG 2 cut(s) 61, 62
AsuHPI GGTGA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 61
BccI CCATC 1 cut(s) 507
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 2 cut(s) 61, 62
BcoDI GTCTC 1 cut(s) 369
BfaI CTAG 4 cut(s) 72, 194, 269, 404
BfmI CTRYAG 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 370
Bme1390I CCNGG 3 cut(s) 61, 62, 134
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 57, 58
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 3 cut(s) 61, 62, 134
BmsI GCATC 1 cut(s) 529
BpuMI CCSGG 2 cut(s) 61, 62
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 512
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 60
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 4 cut(s) 59, 137, 267, 399
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 3 cut(s) 61, 92, 524
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 369
BsnI GGCC 4 cut(s) 59, 137, 267, 399
BsoBI CYCGRG 1 cut(s) 60
Bsp120I GGGCCC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 61
Bsp13I TCCGGA 1 cut(s) 523
BspANI GGCC 4 cut(s) 59, 137, 267, 399
BspCNI CTCAG 1 cut(s) 193
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 370
BsrI ACTGG 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 134
BstAFI CTTAAG 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 286
BstC8I GCNNGC 2 cut(s) 282, 336
BstDEI CTNAG 2 cut(s) 170, 180
BstFNI CGCG 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 369
BstMWI GCNNNNNNNGC 2 cut(s) 134, 286
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 3 cut(s) 59, 60, 132
BstSFI CTRYAG 1 cut(s) 23
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 1 cut(s) 160
BsuRI GGCC 4 cut(s) 59, 137, 267, 399
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 1 cut(s) 233
Cac8I GCNNGC 2 cut(s) 282, 336
Cfr13I GGNCC 2 cut(s) 57, 58
Cfr9I CCCGGG 1 cut(s) 60
Csp6I GTAC 2 cut(s) 156, 167
CviAII CATG 3 cut(s) 239, 286, 299
CviQI GTAC 2 cut(s) 156, 167
DdeI CTNAG 2 cut(s) 170, 180
Eco147I AGGCCT 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 24, 193
Eco88I CYCGRG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 3 cut(s) 242, 289, 302
FaiI YATR 9 cut(s) 19, 150, 240, 287, 300, 350, 435, 465, 552
FatI CATG 3 cut(s) 238, 285, 298
FriOI GRGCYC 1 cut(s) 61
FspBI CTAG 4 cut(s) 72, 194, 269, 404
HaeIII GGCC 4 cut(s) 59, 137, 267, 399
HapII CCGG 3 cut(s) 61, 92, 524
Hin1II CATG 3 cut(s) 242, 289, 302
HindIII AAGCTT 2 cut(s) 110, 425
HinfI GANTC 1 cut(s) 488
HpaII CCGG 3 cut(s) 61, 92, 524
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 3 cut(s) 310, 503, 524
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 92, 217, 410
HpyCH4V TGCA 5 cut(s) 238, 280, 289, 334, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 286
HpyF3I CTNAG 2 cut(s) 170, 180
Hsp92II CATG 3 cut(s) 242, 289, 302
Kpn2I TCCGGA 1 cut(s) 523
LmnI GCTCC 1 cut(s) 526
LweI GCATC 1 cut(s) 529
MaeI CTAG 4 cut(s) 72, 194, 269, 404
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 3 cut(s) 272, 324, 527
MnlI CCTC 6 cut(s) 7, 88, 148, 155, 409, 450
MroI TCCGGA 1 cut(s) 523
MseI TTAA 5 cut(s) 114, 371, 380, 422, 516
MspCI CTTAAG 1 cut(s) 370
MspI CCGG 3 cut(s) 61, 92, 524
MspR9I CCNGG 3 cut(s) 61, 62, 134
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 134, 286
NciI CCSGG 2 cut(s) 61, 62
NlaIII CATG 3 cut(s) 242, 289, 302
NlaIV GGNNCC 1 cut(s) 59
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 242
PceI AGGCCT 1 cut(s) 399
PfeI GAWTC 1 cut(s) 488
PshBI ATTAAT 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 59
PspOMI GGGCCC 1 cut(s) 57
PspPI GGNCC 2 cut(s) 57, 58
RsaI GTAC 2 cut(s) 157, 168
RsaNI GTAC 2 cut(s) 156, 167
SaqAI TTAA 5 cut(s) 114, 371, 380, 422, 516
Sau96I GGNCC 2 cut(s) 57, 58
ScrFI CCNGG 3 cut(s) 61, 62, 134
SduI GDGCHC 1 cut(s) 61
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 23
SmaI CCCGGG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SrfI GCCCGGGC 1 cut(s) 62
Sse9I AATT 3 cut(s) 272, 324, 527
SseBI AGGCCT 1 cut(s) 399
SspMI CTAG 4 cut(s) 72, 194, 269, 404
StuI AGGCCT 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 59, 60, 132
TaqI TCGA 2 cut(s) 243, 309
TasI AATT 3 cut(s) 272, 324, 527
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 5 cut(s) 114, 371, 380, 422, 516
Tru9I TTAA 5 cut(s) 114, 371, 380, 422, 516
TscAI CASTG 1 cut(s) 240
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspMI CCCGGG 1 cut(s) 60
TspRI CASTG 1 cut(s) 240
Vha464I CTTAAG 1 cut(s) 370
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 1 cut(s) 527
XceI RCATGY 1 cut(s) 242
XmaI CCCGGG 1 cut(s) 60
XspI CTAG 4 cut(s) 72, 194, 269, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.