RchiOBHm_Chr5g0038961

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010536
Physical Location & Seq
Reverse (-)
33706447 .. 33707016
570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31754

Sequence Viewer

Length: 570 bp
ATGGCCAGGCATGATGAGTACATGCAAGCTAACCGGTCTGCTGCCATCTCTTTTCAAGACCGATCACAACCCAAGAAGCACTGGACTCCCCCAGCTGATGGTTTCTTAAAGTTGAATGCGGATGGTTCCTTCCTGCCTTCAATCCCGTATGGTGGTACGGGGGGTGTAATTAGAGACTCCCATGGTCAGTTCATTGCTGGCTTCTCCTGTAGAATGGAGTTTGTTAGCTCCCCGTTACACATTGAGTTACTTGCTTTGAAGAATGGATTAGAGTTGCTTCAAGCTATGCAGATCACACGAGCTGTAGTAGAGAGTGACTGCTTACTTGCGGTGCAGGCTATTAACTTAACAGTTGGGGAACTCTCCTCTTTGAGTGCCTTGATCACAGACCTCCAGTCTCTATTAGCTGCAACTACAGAAGTTGAGCTCTATTTTGTCCCCCGTCAGGCTAATGTTGTAGCACATAGACTAGCTAGCTGTAGCTATGAATCTAATATCAATGTTGATTGGTTTGTTAATGCTCCAAAATTCATCTCGGATGCCCTCATGTACGATTTTCATCGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

189

Amino Acids

20.86

Weight (kDa)

5.61

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 39 - 159 7e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AciI CCGC 2 cut(s) 119, 329
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 527
AfaI GTAC 3 cut(s) 20, 157, 551
AfiI CCNNNNNNNGG 3 cut(s) 98, 152, 445
AgeI ACCGGT 1 cut(s) 33
AgsI TTSAA 5 cut(s) 56, 115, 141, 259, 281
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 1 cut(s) 5
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 168, 402
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 41, 407
ApoI RAATTY 1 cut(s) 527
AsiGI ACCGGT 1 cut(s) 33
AsuNHI GCTAGC 1 cut(s) 473
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 429
BauI CACGAG 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 28, 394
BccI CCATC 3 cut(s) 53, 92, 116
BciT130I CCWGG 1 cut(s) 7
BclI TGATCA 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 168, 402
BfaI CTAG 2 cut(s) 470, 474
BfmI CTRYAG 4 cut(s) 208, 303, 414, 478
BisI GCNGC 2 cut(s) 42, 408
BlsI GCNGC 2 cut(s) 43, 409
Bme1390I CCNGG 1 cut(s) 7
BmeRI GACNNNNNGTC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 7
BmsI GCATC 1 cut(s) 529
BmtI GCTAGC 1 cut(s) 477
BpmI CTGGAG 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 152, 445
Bse118I RCCGGY 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 86, 394
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 2 cut(s) 127, 544
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 3 cut(s) 98, 152, 445
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 86, 394
BseRI GAGGAG 1 cut(s) 355
BseXI GCAGC 2 cut(s) 28, 394
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 353
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 3 cut(s) 98, 152, 445
BsmAI GTCTC 2 cut(s) 168, 402
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 3 cut(s) 62, 291, 381
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 2 cut(s) 119, 329
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 1 cut(s) 127
BspOI GCTAGC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 33
BsrI ACTGG 2 cut(s) 86, 394
BssAI RCCGGY 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 3 cut(s) 62, 291, 381
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 181
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 27, 199, 336, 475
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 2 cut(s) 127, 544
BstKTI GATC 3 cut(s) 65, 294, 384
BstMAI GTCTC 2 cut(s) 168, 402
BstMBI GATC 3 cut(s) 62, 291, 381
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 7
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 5
BstSFI CTRYAG 4 cut(s) 208, 303, 414, 478
BstV1I GCAGC 2 cut(s) 28, 394
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 2 cut(s) 127, 544
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 4 cut(s) 27, 199, 336, 475
Cfr10I RCCGGY 1 cut(s) 33
Csp6I GTAC 3 cut(s) 19, 156, 550
CspAI ACCGGT 1 cut(s) 33
CviAII CATG 4 cut(s) 11, 22, 182, 547
CviQI GTAC 3 cut(s) 19, 156, 550
DpnI GATC 3 cut(s) 64, 293, 383
DpnII GATC 3 cut(s) 62, 291, 381
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 4 cut(s) 14, 25, 185, 550
FaiI YATR 8 cut(s) 12, 23, 150, 183, 287, 465, 486, 548
FaqI GGGAC 1 cut(s) 422
FatI CATG 4 cut(s) 10, 21, 181, 546
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 42, 408
FokI GGATG 2 cut(s) 134, 551
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 2 cut(s) 42, 408
FspBI CTAG 2 cut(s) 470, 474
GluI GCNGC 2 cut(s) 42, 408
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 34
Hin1II CATG 4 cut(s) 14, 25, 185, 550
HinfI GANTC 3 cut(s) 85, 176, 488
HpaII CCGG 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 538
Hpy188III TCNNGA 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 139, 147
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 4 cut(s) 25, 289, 334, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
Hsp92II CATG 4 cut(s) 14, 25, 185, 550
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 3 cut(s) 62, 291, 381
LmnI GCTCC 2 cut(s) 233, 526
Lsp1109I GCAGC 2 cut(s) 28, 394
LweI GCATC 1 cut(s) 529
MaeI CTAG 2 cut(s) 470, 474
MaeIII GTNAC 3 cut(s) 234, 246, 314
MalI GATC 3 cut(s) 64, 293, 383
MboI GATC 3 cut(s) 62, 291, 381
MboII GAAGA 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 168, 527
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 79, 170
MnlI CCTC 3 cut(s) 376, 401, 554
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 107, 342, 347, 516
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 7
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 335
NcoI CCATGG 1 cut(s) 181
NdeII GATC 3 cut(s) 62, 291, 381
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 4 cut(s) 14, 25, 185, 550
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 314
NspI RCATGY 1 cut(s) 25
PctI GAATGC 1 cut(s) 121
PfeI GAWTC 1 cut(s) 488
PflMI CCANNNNNTGG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 33
PkrI GCNGC 2 cut(s) 43, 409
PleI GAGTC 2 cut(s) 79, 170
PpsI GAGTC 2 cut(s) 79, 170
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 5
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 3 cut(s) 20, 157, 551
RsaNI GTAC 3 cut(s) 19, 156, 550
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 107, 342, 347, 516
SatI GCNGC 2 cut(s) 42, 408
Sau3AI GATC 3 cut(s) 62, 291, 381
SchI GAGTC 2 cut(s) 79, 170
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 4 cut(s) 208, 303, 414, 478
Sse9I AATT 2 cut(s) 168, 527
SsiI CCGC 2 cut(s) 119, 329
SspMI CTAG 2 cut(s) 470, 474
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 352
TaqII GACCGA 1 cut(s) 75
TasI AATT 2 cut(s) 168, 527
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 4 cut(s) 107, 342, 347, 516
Tru9I TTAA 4 cut(s) 107, 342, 347, 516
TscAI CASTG 1 cut(s) 86
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 41, 407
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 4 cut(s) 181, 501, 520, 548
TspRI CASTG 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 98
XapI RAATTY 1 cut(s) 527
XceI RCATGY 1 cut(s) 25
XspI CTAG 2 cut(s) 470, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.