RchiOBHm_Chr4g0413301

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010536
Physical Location & Seq
Forward (+)
37562595 .. 37563300
706 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38383

Sequence Viewer

Length: 357 bp
ATGGATTTTGTTAGCTCACCCTTACATGCTGAGTTGCTTGCCTTGAAGAATGGGCTGGAATTGCTGCAGGCTATGAATATTACTCGTGCCACTATTGAAAGTGATTGTTTGGTTGCTGTGCAGGCTATAAATTCCTTAACCCCAGACCTCTCTCCCTTGGGTGCGTTGATTGTGGATGTTCAAGAGCTGTTAGCTGCCAGTTTGGACATCAAACTCTCCCATGTTCCACGCCAAGCTAACACTGTGGCGCATAGGTTGGCTAGTTATAGCTTTGAATGTAATGTTCATCTTGAATGGTTCTCTATTGCTCCAGAATTCATCCTGGATGCCCTTTTGTATGATTTCAATCGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

118

Amino Acids

13.02

Weight (kDa)

4.65

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 5 - 89 2.2e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 130, 314
AgsI TTSAA 6 cut(s) 46, 98, 182, 275, 293, 346
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 5 cut(s) 15, 187, 194, 236, 270
AluI AGCT 5 cut(s) 15, 187, 194, 236, 270
ApeKI GCWGC 2 cut(s) 64, 194
ApoI RAATTY 2 cut(s) 130, 314
AspLEI GCGC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 9
BauI CACGAG 1 cut(s) 84
BbvI GCAGC 2 cut(s) 51, 181
BciT130I CCWGG 1 cut(s) 323
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 65
BisI GCNGC 2 cut(s) 65, 195
BlsI GCNGC 2 cut(s) 66, 196
Bme1390I CCNGG 1 cut(s) 323
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 294
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 3 cut(s) 181, 318, 331
BseJI GATNNNNATC 1 cut(s) 345
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 51, 181
BsgI GTGCAG 1 cut(s) 140
BspCNI CTCAG 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 69
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 156
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 244
BstC8I GCNNGC 3 cut(s) 39, 69, 123
BstDEI CTNAG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 181, 318, 331
BstHHI GCGC 1 cut(s) 250
BstMWI GCNNNNNNNGC 2 cut(s) 61, 122
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 51, 181
BtsCI GGATG 3 cut(s) 181, 318, 331
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 3 cut(s) 39, 69, 123
CfoI GCGC 1 cut(s) 250
CviAII CATG 2 cut(s) 26, 221
CviJI RGCY 9 cut(s) 15, 55, 71, 125, 187, 194, 236, 260, 270
CviKI_1 RGCY 9 cut(s) 15, 55, 71, 125, 187, 194, 236, 260, 270
DdeI CTNAG 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 2 cut(s) 29, 224
FaiI YATR 7 cut(s) 27, 74, 128, 222, 252, 267, 339
FatI CATG 2 cut(s) 25, 220
Fnu4HI GCNGC 2 cut(s) 65, 195
FokI GGATG 3 cut(s) 188, 305, 338
Fsp4HI GCNGC 2 cut(s) 65, 195
FspBI CTAG 1 cut(s) 261
GlaI GCGC 1 cut(s) 249
GluI GCNGC 2 cut(s) 65, 195
GsuI CTGGAG 1 cut(s) 294
HhaI GCGC 1 cut(s) 250
Hin1II CATG 2 cut(s) 29, 224
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HphI GGTGA 1 cut(s) 9
Hpy188III TCNNGA 3 cut(s) 182, 290, 311
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 2 cut(s) 67, 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 122
HpyF3I CTNAG 1 cut(s) 30
Hsp92II CATG 2 cut(s) 29, 224
HspAI GCGC 1 cut(s) 248
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 8 cut(s) 41, 53, 107, 156, 211, 308, 324, 335
Lsp1109I GCAGC 2 cut(s) 51, 181
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 261
MboII GAAGA 1 cut(s) 58
MluCI AATT 3 cut(s) 59, 130, 314
MnlI CCTC 1 cut(s) 158
MseI TTAA 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 61, 122
NlaIII CATG 2 cut(s) 29, 224
NspI RCATGY 1 cut(s) 29
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 2 cut(s) 66, 196
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PstI CTGCAG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 2 cut(s) 65, 195
ScrFI CCNGG 1 cut(s) 323
SetI ASST 7 cut(s) 17, 150, 189, 196, 238, 257, 272
SfaNI GCATC 1 cut(s) 316
SfcI CTRYAG 1 cut(s) 65
Sse9I AATT 3 cut(s) 59, 130, 314
SspI AATATT 1 cut(s) 79
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 244
TasI AATT 3 cut(s) 59, 130, 314
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TscAI CASTG 1 cut(s) 247
TseI GCWGC 2 cut(s) 64, 194
TspDTI ATGAA 3 cut(s) 89, 275, 307
TspRI CASTG 1 cut(s) 247
XapI RAATTY 2 cut(s) 130, 314
XceI RCATGY 1 cut(s) 29
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.