Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0008065

Physical Location & Seq

Genomic Coordinates Forward (+)

3513835 .. 3518325

Gene Structure 4491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g06100.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3477 bp

ATGGAGTCTCACAGAGTCAGTTTGGCCATAGTCATAGTCTGTTCTGCTCTGCTTACTGTTCTATCAGCTCAGACAAGCTTGGAGGTTGAAGTCGAGGCCTTGAAGGCGTTCAAGAAATCCATCACCAGCGACCCCAATGGAACACTTACAGACTGGACCTCAGAGGCAAGCCATCACTGCAATTGGTCTGGCATTGCCTGTGACCCCTCAACGAATCAAGTTACCTCTATTTCTCTGGTGGAAAAGCAGCTCGCAGGCGTGATCTCACCCTTCCTTGGAAACATTTCAGGCCTCCAGGTTCTTGATCTAACTTCAAACTCATTCACAGGACATATTCCAGCTGAGCTGGGACTATGCTCCCAACTTTCCCAACTTGCTCTGTATCAAAATTCTCTTTCGGGTTCAATTCCACCAGAACTAGGAAACCTTGGTAATCTGCAGTCATTAGATTTGGGGGATAACTTTTTGAGTGGAAGCATACCAGAGAGCATTTGCAACTGCAGAAACTTGTCTTTGGTTGGTGCGGAATTTAATAACCTCACCGGCAAAATCCCATCAAATATAGGAAACCTTGTTAATCTTCAGCTTCTTCTAGCAAATGGCAACAACTTTACAGGCTCATTACCTGCTTCCATGGGTAAGTTGGCAGCTTTCAAAGCAGTTGATTTAAGTAAAAACCAGTTGTCCGGTGCACTACCTAGGGAGCTAGGGAATCTGTCAAATTTAGAACAACTGGTAGTGTTTGAAAATTCCTTTGTTGGGGAAATCCCTTCTGAACTAAGTTGGTGTAAGAAGCTAGTTAACCTTGAAATTTACAGCAATCATTTCACCGGAAGCATTCCCCCTGAGCTTGGAAGTTTGGTTCACTTGGAAACATTGAGATTGTACAAGAATAGGCTAAACTCCACAATCCCACTCTCCATTTTTCAGCTGAAATCATTAACCCATTTAGAACTTTCAAACAATGAGTTAAGCGGGACAATACCATCCGAGCTTGGATCTTTGAGATCATTGCAAATGTTGACCCTGCACTCCAATAAGTTTACTGGAAAGATACCCTCATCCTTGACAAGCTTGACAAATCTGACATATTTGTCAATGAGCTTAAATTCTCTAACAGGGGAGCTTCCATCAAACATAGGGTCGCTTTATAATCTCAAAAACCTCTCCATGAATGGCAACCTTCTTGAGGGATCCATACCATCTAGTATTACCAACTGCACCAATCTTCAAGTTATAAGCTTGGCTATTAATAGAATAACTGGGAAAATACCTCAGGGTCTAGGGCAGCTTCAAAATCTTAGTTTCTTTTCTGTTTGGTCAAATAAATTGTTCGGAGAAATCCCAGATGACCTCTTCAATTGCACAACTCTTTCCACTCTAGATTTGGGCCTGAACAACTTTAGCGGTTATCTGAAACCTCGAATTGGAAAACTTTCCAATCTCAGGCGTTTGAAAGCCTTCGCTAATTCATTTGTCGGAAAAATTCCACCAGAGATTGGCCAACTGAACCAACTTATCGTCCTTGACCTTGGTGAAAATAGATTCTCAGGTCCAGTTCCACCACAGCTGTCTAATCTCTCACACCTACAAGGGCTTTCCCTGGACAACAATGCTTTAGAAGGAGCCATCCCCGAGAAACTTTTTGAGCTAAAAGAGTTAACTAAACTTGAGTTGCAGCAGAACAAACTGATTGGTCCAATTCCAGATAGTGTCTCTAAACTTGAGTTGCTTTCATACTTAAACCTCCAAGGTAACATGCTCAACGGTTCCATTCCGAAAAGCATGGCTCACCTTAATCGGCTAACGACTGTGGATCTCTCTCACAATCATCTCTCAGGACCCATTCCAGGGTCTGTGGTCTCAGGGATGAAAAGCATGCAGATATATTTGAATTTCTCATACAATTTCTTGGATGGCAGCATTCCAGACGAGCTTGGCATGTTGGGAATGGTGCAGGCCATTGACATTTCCAACAACAATTTTTCAGGAATGATTCCTAGAGCGCTAGAAGGCTGCAGGAACTTGTACTCTCTTGATCTGTCAGGTAACAAACTTTCTGGGCCAATTCAAGCTGAAGTTTTTGCCAAGATGGACACCCTCACAAGCTTGAACCTTTCAAGGAACAACTTAGATGGTGAACTCCCAGAAGAGTTGACAAATTTGAAGCATCTTAGCTCCCTTGATCTTTCCCAGAATTCTCTGAGGGGAGTTATCCCTGAGGGCTTTTCCAATTTTACCACCACCTTGAAACACTTGAACCTCTCTTTCAATCAACTTGAAGGCCCTCTTCCAGACACCGGACTATTCAGAAGTATGAATGCATCCAGCTTGGTAGGAAACCCAGACCTCTGTGGAGACATACTGCTGAAGACATGCAAGAAAAGTTCACGGATATCAAAGACAACCATGTACGTTCTCGTGTCGCTTGGAATTGCTTCTGTGCTTCTGATTCTAGTGATCATTTTTCTATTCCTCAACCGATTCAACAAATTGCGTAAGCAAGAAAAGGTTGAAAATCCAGAACTAGAATGTGCTACAGCTTTGACACTCAAGAGGTTTGATCCAAAGGATTTAGAAAATGCCACTGGTCGTTTCAGCAAAGAGAACATTCTAGGTGCAAGCAGCTTGAGCACAGTGTACAAAGGTGGACTAGAAGATGGGCAGATAGTAGCTATAAAGAGTTTGAATCTCCAGCAATTCTCAGTTGAGTCAGATAAATGCTTCAACAGAGAGATCAAGACACTGAGTCAACTAAGGCATAGGAATTTGGTGAAGGTACTTGGCTATGCATGGGAGAGCGGGAAATTAAAGGCCTTAGTTCTGGAGTACATGGAGAACGGGAACCTGGAGAACATTATACATGAAGATGGGATGAATCAGTGGAGATTGACATTCTCTCAAAGAATCAATATTCTAGTCTCTATAGCAAGTGGATTGGATTACCTTCATTCAGGCTATGATTTTCCAATTGTTCATTGCGACTTGAAGCCTTCTAACATCTTGTTGGATGATGATTGGGAGGCCCATGTGAGTGATTTTGGAACAGCTAGAATGTTGGGTGTTCATCTCCAGGATGGGAGTAGCCTTTCCACGGCATCAGCTTTTCAAGGCACAATTGGCTACTTAGCACCAGAGTTTGCATACATGAGGAAAATCACAACCAAGGTAGACGTGTTCAGCTTTGGCGTAATAGTGATGGAGTTACTGACAAGACAAAGGCCAACAGGAATCATGGAAGAAAATGGGCAGCCAATGAGCTTGCACCAACTAGTGGAGAAAGCCCTTGCAAATGGATCACATTCGCTTCTTGAAGTTCTGGATCCAATGCTAACTTCAAATATCTCCAAGGTACAAGAGGCTAGTGCAGAAGAGCTGCTCAAGCTAGCCTTGATTTGCACCAACCAAATTCCCGAGGAGAGACCTATCATGAATGAGGTGTTGTCTGCCCTTAAGAAGCTGAAAAAGGCAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006898 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010359 GO:0012505 GO:0016020 GO:0016043 GO:0016045 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031410 GO:0031982 GO:0032409 GO:0032412 GO:0032879 GO:0033036 GO:0033037 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0042545 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065009 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098542 GO:0098543 GO:0098581 GO:0098657 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1159

Amino Acids

126.36

Weight (kDa)

5.89

Isoelectric Point (pI)

27.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 68	3.3e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	182 - 274	6.7e-07	Leucine-rich repeat region
LRR_14	PF23598	278 - 370	3.5e-10	Leucine-rich repeat region
LRR_8	PF13855	289 - 348	9.8e-08	Leucine rich repeat
LRR_14	PF23598	355 - 465	5.4e-08	Leucine-rich repeat region
LRR_14	PF23598	455 - 559	1.2e-07	Leucine-rich repeat region
LRR_8	PF13855	553 - 612	1.6e-07	Leucine rich repeat
LRR_8	PF13855	653 - 711	6e-09	Leucine rich repeat
LRR_8	PF13855	674 - 735	2e-08	Leucine rich repeat
LRR_8	PF13855	700 - 760	2.4e-06	Leucine rich repeat
Pkinase	PF00069	867 - 1149	5.2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	869 - 1151	5e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46330 AT5G46330
fragaria_vesca	FvH4_3g06100
malus_domestica	MD05G1289000.v1.1
prunus_persica	Prupe.4G076500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0013311
rosa_laevigata	RLG00000032007 RLG00000032011
rosa_multiflora	Rmu_co8051088.1_g000001 Rmu_sc0003458.1_g000007 Rmu_sc0003458.1_g000011 Rmu_sc0004521.1_g000021 Rmu_sc0007948.1_g000008
rosa_roxburghii	Rroxscaffold_1G00063230
rosa_rugosa	Rorug05G0008800
rosa_samantha	Rh5AG103400 Rh5AG103500 Rh5AG104200 Rh5BG100200 Rh5CG111400 Rh5CG111800 Rh5DG099000
rosa_wichuraiana	Rw5G009030 Rw5G009070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1152, 1238
AasI	GACNNNNNNGTC	1 cut(s)	1093
Acc36I	ACCTGC	1 cut(s)	634
AccB7I	CCANNNNNTGG	2 cut(s)	1499, 3274
AccBSI	CCGCTC	1 cut(s)	2802
AccI	GTMKAC	1 cut(s)	3171
AciI	CCGC	4 cut(s)	524, 975, 1407, 2802
AclWI	GGATC	8 cut(s)	1006, 1188, 1201, 1824, 2558, 3304, 3317, 3330
AcoI	YGGCCR	2 cut(s)	24, 1501
AcuI	CTGAAG	3 cut(s)	566, 2097, 2390
AfaI	GTAC	7 cut(s)	887, 2030, 2414, 2642, 2781, 2831, 3354
AfeI	AGCGCT	1 cut(s)	2007
AflII	CTTAAG	1 cut(s)	3452
AflIII	ACRYGT	1 cut(s)	3174
AhdI	GACNNNNNGTC	1 cut(s)	2748
AhlI	ACTAGT	1 cut(s)	3271
AjiI	CACGTC	1 cut(s)	3175
AjnI	CCWGG	5 cut(s)	294, 1602, 1849, 2847, 3072
AjuI	GAANNNNNNNTTGG	2 cut(s)	3319, 3351
AloI	GAACNNNNNNTCC	2 cut(s)	846, 878
Alw21I	GWGCWC	2 cut(s)	694, 2636
Alw26I	GTCTC	6 cut(s)	12, 1720, 1867, 2352, 2926, 3415
Alw44I	GTGCAC	1 cut(s)	690
AlwI	GGATC	8 cut(s)	1006, 1188, 1201, 1824, 2558, 3304, 3317, 3330
AlwNI	CAGNNNCTG	1 cut(s)	1856
Ama87I	CYCGRG	2 cut(s)	1634, 3413
Aor51HI	AGCGCT	1 cut(s)	2007
ApaLI	GTGCAC	1 cut(s)	690
ApeKI	GCWGC	9 cut(s)	247, 647, 1288, 1678, 1920, 2016, 2625, 3250, 3376
ArsI	GACNNNNNNTTYG	2 cut(s)	1127, 1159
AseI	ATTAAT	1 cut(s)	1251
Asp700I	GAANNNNTTC	5 cut(s)	107, 283, 1435, 1460, 2437
AspA2I	CCTAGG	1 cut(s)	698
AspLEI	GCGC	1 cut(s)	2008
AspS9I	GGNCC	8 cut(s)	156, 1390, 1553, 1697, 1841, 2063, 2285, 3025
AsuHPI	GGTGA	8 cut(s)	115, 258, 532, 820, 1547, 1784, 2150, 2785
AsuNHI	GCTAGC	1 cut(s)	3385
AvaI	CYCGRG	2 cut(s)	1634, 3413
AvaII	GGWCC	4 cut(s)	156, 1553, 1697, 1841
AvrII	CCTAGG	1 cut(s)	698
AxyI	CCTNAGG	2 cut(s)	1275, 2220
BaeGI	GKGCMC	1 cut(s)	694
BalI	TGGCCA	2 cut(s)	26, 1503
BamHI	GGATCC	2 cut(s)	1193, 3322
BauI	CACGAG	1 cut(s)	2420
BbsI	GAAGAC	1 cut(s)	2378
Bbv12I	GWGCWC	2 cut(s)	694, 2636
BbvI	GCAGC	9 cut(s)	259, 659, 1300, 1690, 1932, 2003, 2637, 3262, 3363
BceAI	ACGGC	1 cut(s)	3111
BciT130I	CCWGG	5 cut(s)	296, 1604, 1851, 2849, 3074
BclI	TGATCA	1 cut(s)	2460
BcoDI	GTCTC	6 cut(s)	12, 1720, 1867, 2352, 2926, 3415
BcuI	ACTAGT	1 cut(s)	3271
BfmI	CTRYAG	5 cut(s)	437, 499, 2017, 2538, 2925
BfoI	RGCGCY	1 cut(s)	2009
BfrI	CTTAAG	1 cut(s)	3452
BfuAI	ACCTGC	1 cut(s)	634
BglI	GCCNNNNNGGC	1 cut(s)	104
BisI	GCNGC	9 cut(s)	248, 648, 1289, 1679, 1921, 2017, 2626, 3251, 3377
BlnI	CCTAGG	1 cut(s)	698
BlpI	GCTNAGC	1 cut(s)	342
BlsI	GCNGC	9 cut(s)	249, 649, 1290, 1680, 1922, 2018, 2627, 3252, 3378
Bme1390I	CCNGG	5 cut(s)	296, 1604, 1851, 2849, 3074
Bme18I	GGWCC	4 cut(s)	156, 1553, 1697, 1841
BmeRI	GACNNNNNGTC	1 cut(s)	2748
BmeT110I	CYCGRG	2 cut(s)	1634, 3413
BmgBI	CACGTC	1 cut(s)	3175
BmgT120I	GGNCC	8 cut(s)	156, 1390, 1553, 1697, 1841, 2063, 2285, 3025
BmiI	GGNNCC	6 cut(s)	1195, 1627, 1771, 1843, 2846, 3324
BmrFI	CCNGG	5 cut(s)	296, 1604, 1851, 2849, 3074
BmrI	ACTGGG	1 cut(s)	1272
BmsI	GCATC	3 cut(s)	2179, 2333, 3107
BmtI	GCTAGC	1 cut(s)	3389
BmuI	ACTGGG	1 cut(s)	1272
BpiI	GAAGAC	1 cut(s)	2378
BpmI	CTGGAG	5 cut(s)	278, 2678, 2846, 2870, 3056
Bpu10I	CCTNAGC	1 cut(s)	846
Bpu1102I	GCTNAGC	1 cut(s)	342
BpuEI	CTTGAG	6 cut(s)	1208, 1691, 1745, 2537, 2650, 3365
BsaI	GGTCTC	2 cut(s)	1867, 3415
BsaWI	WCCGGW	3 cut(s)	686, 830, 2300
Bse118I	RCCGGY	1 cut(s)	542
Bse1I	ACTGG	7 cut(s)	158, 679, 738, 1051, 1267, 1556, 2593
Bse21I	CCTNAGG	2 cut(s)	1275, 2220
Bse3DI	GCAATG	3 cut(s)	192, 1010, 2977
BseBI	CCWGG	5 cut(s)	296, 1604, 1851, 2849, 3074
BseGI	GGATG	9 cut(s)	986, 1061, 1629, 1875, 1921, 2324, 2880, 3016, 3082
BseMI	GCAATG	3 cut(s)	192, 1010, 2977
BseNI	ACTGG	7 cut(s)	158, 679, 738, 1051, 1267, 1556, 2593
BseRI	GAGGAG	1 cut(s)	3431
BseSI	GKGCMC	1 cut(s)	694
BseXI	GCAGC	9 cut(s)	259, 659, 1300, 1690, 1932, 2003, 2637, 3262, 3363
BseYI	CCCAGC	1 cut(s)	346
BsgI	GTGCAG	4 cut(s)	1013, 1204, 1976, 3387
BsiHKAI	GWGCWC	2 cut(s)	694, 2636
BsiHKCI	CYCGRG	2 cut(s)	1634, 3413
BsiSI	CCGG	4 cut(s)	543, 687, 831, 2301
BslFI	GGGAC	2 cut(s)	363, 991
BsmAI	GTCTC	6 cut(s)	12, 1720, 1867, 2352, 2926, 3415
BsmFI	GGGAC	2 cut(s)	363, 991
BsmI	GAATGC	3 cut(s)	837, 1923, 2326
Bso31I	GGTCTC	2 cut(s)	1867, 3415
BsoBI	CYCGRG	2 cut(s)	1634, 3413
Bsp1286I	GDGCHC	2 cut(s)	694, 2636
Bsp1407I	TGTACA	2 cut(s)	885, 2640
Bsp1720I	GCTNAGC	1 cut(s)	342
Bsp19I	CCATGG	1 cut(s)	633
BspACI	CCGC	4 cut(s)	524, 975, 1407, 2802
BspHI	TCATGA	1 cut(s)	3429
BspLI	GGNNCC	6 cut(s)	1195, 1627, 1771, 1843, 2846, 3324
BspMAI	CTGCAG	3 cut(s)	441, 503, 2021
BspMI	ACCTGC	1 cut(s)	634
BspOI	GCTAGC	1 cut(s)	3389
BspPI	GGATC	8 cut(s)	1006, 1188, 1201, 1824, 2558, 3304, 3317, 3330
BspQI	GCTCTTC	1 cut(s)	3366
BspTI	CTTAAG	1 cut(s)	3452
BspTNI	GGTCTC	2 cut(s)	1867, 3415
BsrBI	CCGCTC	1 cut(s)	2802
BsrDI	GCAATG	3 cut(s)	192, 1010, 2977
BsrFI	RCCGGY	1 cut(s)	542
BsrGI	TGTACA	2 cut(s)	885, 2640
BsrI	ACTGG	7 cut(s)	158, 679, 738, 1051, 1267, 1556, 2593
BssAI	RCCGGY	1 cut(s)	542
BssSI	CACGAG	1 cut(s)	2420
BssT1I	CCWWGG	8 cut(s)	274, 427, 633, 698, 1531, 1750, 3165, 3348
Bst2BI	CACGAG	1 cut(s)	2420
Bst2UI	CCWGG	5 cut(s)	296, 1604, 1851, 2849, 3074
Bst4CI	ACNGT	4 cut(s)	58, 1769, 1813, 2638
Bst6I	CTCTTC	4 cut(s)	1361, 2145, 2295, 3366
BstAFI	CTTAAG	1 cut(s)	3452
BstAUI	TGTACA	2 cut(s)	885, 2640
BstC8I	GCNNGC	8 cut(s)	169, 252, 256, 1880, 1959, 2623, 3263, 3387
BstDSI	CCRYGG	2 cut(s)	633, 3093
BstENI	CCTNNNNNAGG	1 cut(s)	1187
BstF5I	GGATG	9 cut(s)	986, 1061, 1629, 1875, 1921, 2324, 2880, 3016, 3082
BstH2I	RGCGCY	1 cut(s)	2009
BstHHI	GCGC	1 cut(s)	2008
BstMAI	GTCTC	6 cut(s)	12, 1720, 1867, 2352, 2926, 3415
BstMWI	GCNNNNNNNGC	6 cut(s)	104, 177, 656, 2631, 3120, 3382
BstNI	CCWGG	5 cut(s)	296, 1604, 1851, 2849, 3074
BstNSI	RCATGY	4 cut(s)	1762, 1882, 1945, 2379
BstSCI	CCNGG	5 cut(s)	294, 1602, 1849, 2847, 3072
BstSFI	CTRYAG	5 cut(s)	437, 499, 2017, 2538, 2925
BstSLI	GKGCMC	1 cut(s)	694
BstV1I	GCAGC	9 cut(s)	259, 659, 1300, 1690, 1932, 2003, 2637, 3262, 3363
BstV2I	GAAGAC	1 cut(s)	2378
BstX2I	RGATCY	4 cut(s)	998, 1193, 1816, 3322
BstYI	RGATCY	4 cut(s)	998, 1193, 1816, 3322
Bsu36I	CCTNAGG	2 cut(s)	1275, 2220
BtgI	CCRYGG	2 cut(s)	633, 3093
BtrI	CACGTC	1 cut(s)	3175
BtsCI	GGATG	9 cut(s)	986, 1061, 1629, 1875, 1921, 2324, 2880, 3016, 3082
BtsI	GCAGTG	1 cut(s)	175
BtsIMutI	CAGTG	5 cut(s)	175, 2586, 2643, 2744, 2888
BveI	ACCTGC	1 cut(s)	634
Cac8I	GCNNGC	8 cut(s)	169, 252, 256, 1880, 1959, 2623, 3263, 3387
CaiI	CAGNNNCTG	1 cut(s)	1856
CciI	TCATGA	1 cut(s)	3429
CfoI	GCGC	1 cut(s)	2008
Cfr10I	RCCGGY	1 cut(s)	542
Cfr13I	GGNCC	8 cut(s)	156, 1390, 1553, 1697, 1841, 2063, 2285, 3025
Csp6I	GTAC	7 cut(s)	886, 2029, 2413, 2641, 2780, 2830, 3353
CviQI	GTAC	7 cut(s)	886, 2029, 2413, 2641, 2780, 2830, 3353
DrdI	GACNNNNNNGTC	1 cut(s)	1093
DriI	GACNNNNNGTC	1 cut(s)	2748
DseDI	GACNNNNNNGTC	1 cut(s)	1093
EaeI	YGGCCR	2 cut(s)	24, 1501
Eam1104I	CTCTTC	4 cut(s)	1361, 2145, 2295, 3366
Eam1105I	GACNNNNNGTC	1 cut(s)	2748
EarI	CTCTTC	4 cut(s)	1361, 2145, 2295, 3366
Eco130I	CCWWGG	8 cut(s)	274, 427, 633, 698, 1531, 1750, 3165, 3348
Eco147I	AGGCCT	3 cut(s)	98, 291, 2816
Eco31I	GGTCTC	2 cut(s)	1867, 3415
Eco32I	GATATC	1 cut(s)	2397
Eco47I	GGWCC	4 cut(s)	156, 1553, 1697, 1841
Eco47III	AGCGCT	1 cut(s)	2007
Eco57I	CTGAAG	3 cut(s)	566, 2097, 2390
Eco81I	CCTNAGG	2 cut(s)	1275, 2220
Eco88I	CYCGRG	2 cut(s)	1634, 3413
EcoNI	CCTNNNNNAGG	1 cut(s)	1187
EcoO109I	RGGNCCY	2 cut(s)	1841, 2285
EcoRI	GAATTC	1 cut(s)	2197
EcoRII	CCWGG	5 cut(s)	294, 1602, 1849, 2847, 3072
EcoRV	GATATC	1 cut(s)	2397
EcoT14I	CCWWGG	8 cut(s)	274, 427, 633, 698, 1531, 1750, 3165, 3348
EcoT22I	ATGCAT	2 cut(s)	2326, 2794
ErhI	CCWWGG	8 cut(s)	274, 427, 633, 698, 1531, 1750, 3165, 3348
FalI	AAGNNNNNCTT	4 cut(s)	2274, 2306, 3374, 3406
FaqI	GGGAC	2 cut(s)	363, 991
FauI	CCCGC	2 cut(s)	968, 2795
FbaI	TGATCA	1 cut(s)	2460
FblI	GTMKAC	1 cut(s)	3171
Fnu4HI	GCNGC	9 cut(s)	248, 648, 1289, 1679, 1921, 2017, 2626, 3251, 3377
FokI	GGATG	9 cut(s)	973, 1048, 1616, 1882, 1928, 2311, 2887, 3023, 3089
Fsp4HI	GCNGC	9 cut(s)	248, 648, 1289, 1679, 1921, 2017, 2626, 3251, 3377
GlaI	GCGC	1 cut(s)	2007
GluI	GCNGC	9 cut(s)	248, 648, 1289, 1679, 1921, 2017, 2626, 3251, 3377
GsaI	CCCAGC	1 cut(s)	350
GsuI	CTGGAG	5 cut(s)	278, 2678, 2846, 2870, 3056
HaeII	RGCGCY	1 cut(s)	2009
HapII	CCGG	4 cut(s)	543, 687, 831, 2301
HhaI	GCGC	1 cut(s)	2008
Hin6I	GCGC	1 cut(s)	2006
HinP1I	GCGC	1 cut(s)	2006
HincII	GTYRAC	5 cut(s)	802, 1023, 1662, 2157, 2753
HindII	GTYRAC	5 cut(s)	802, 1023, 1662, 2157, 2753
HindIII	AAGCTT	4 cut(s)	76, 1072, 1240, 2107
HpaI	GTTAAC	2 cut(s)	802, 1662
HpaII	CCGG	4 cut(s)	543, 687, 831, 2301
HphI	GGTGA	8 cut(s)	115, 258, 532, 820, 1547, 1784, 2150, 2785
HpyCH4III	ACNGT	4 cut(s)	58, 1769, 1813, 2638
HpyCH4IV	ACGT	2 cut(s)	2415, 3174
HpyF10VI	GCNNNNNNNGC	6 cut(s)	104, 177, 656, 2631, 3120, 3382
HpySE526I	ACGT	2 cut(s)	2415, 3174
HspAI	GCGC	1 cut(s)	2006
Ksp22I	TGATCA	1 cut(s)	2460
KspAI	GTTAAC	2 cut(s)	802, 1662
LguI	GCTCTTC	1 cut(s)	3366
LmnI	GCTCC	5 cut(s)	362, 703, 1123, 1625, 2183
Lsp1109I	GCAGC	9 cut(s)	259, 659, 1300, 1690, 1932, 2003, 2637, 3262, 3363
LweI	GCATC	3 cut(s)	2179, 2333, 3107
MaeII	ACGT	2 cut(s)	2415, 3174
MaeIII	GTNAC	5 cut(s)	200, 220, 1754, 2048, 3204
MbiI	CCGCTC	1 cut(s)	2802
MfeI	CAATTG	4 cut(s)	181, 1360, 2970, 3117
MflI	RGATCY	4 cut(s)	998, 1193, 1816, 3322
MhlI	GDGCHC	2 cut(s)	694, 2636
MlsI	TGGCCA	2 cut(s)	26, 1503
MluNI	TGGCCA	2 cut(s)	26, 1503
MlyI	GAGTC	4 cut(s)	14, 24, 2720, 2758
MmeI	TCCRAC	3 cut(s)	1459, 1998, 2988
Mox20I	TGGCCA	2 cut(s)	26, 1503
Mph1103I	ATGCAT	2 cut(s)	2326, 2794
MroXI	GAANNNNTTC	5 cut(s)	107, 283, 1435, 1460, 2437
MscI	TGGCCA	2 cut(s)	26, 1503
MslI	CAYNNNNRTG	2 cut(s)	2868, 3033
Msp20I	TGGCCA	2 cut(s)	26, 1503
MspA1I	CMGCKG	3 cut(s)	341, 931, 1570
MspCI	CTTAAG	1 cut(s)	3452
MspI	CCGG	4 cut(s)	543, 687, 831, 2301
MspR9I	CCNGG	5 cut(s)	296, 1604, 1851, 2849, 3074
MunI	CAATTG	4 cut(s)	181, 1360, 2970, 3117
Mva1269I	GAATGC	3 cut(s)	837, 1923, 2326
MvaI	CCWGG	5 cut(s)	296, 1604, 1851, 2849, 3074
MwoI	GCNNNNNNNGC	6 cut(s)	104, 177, 656, 2631, 3120, 3382
NcoI	CCATGG	1 cut(s)	633
NheI	GCTAGC	1 cut(s)	3385
NlaIV	GGNNCC	6 cut(s)	1195, 1627, 1771, 1843, 2846, 3324
NmuCI	GTSAC	1 cut(s)	200
NsiI	ATGCAT	2 cut(s)	2326, 2794
NspI	RCATGY	4 cut(s)	1762, 1882, 1945, 2379
PaeI	GCATGC	1 cut(s)	1882
PagI	TCATGA	1 cut(s)	3429
PceI	AGGCCT	3 cut(s)	98, 291, 2816
PciSI	GCTCTTC	1 cut(s)	3366
PctI	GAATGC	3 cut(s)	837, 1923, 2326
PdmI	GAANNNNTTC	5 cut(s)	107, 283, 1435, 1460, 2437
PflMI	CCANNNNNTGG	2 cut(s)	1499, 3274
PfoI	TCCNGGA	1 cut(s)	3072
PkrI	GCNGC	9 cut(s)	249, 649, 1290, 1680, 1922, 2018, 2627, 3252, 3378
PleI	GAGTC	4 cut(s)	13, 23, 2719, 2757
PpsI	GAGTC	4 cut(s)	13, 23, 2719, 2757
PpuMI	RGGWCCY	1 cut(s)	1841
PshBI	ATTAAT	1 cut(s)	1251
PsiI	TTATAA	2 cut(s)	1152, 1238
Psp5II	RGGWCCY	1 cut(s)	1841
Psp6I	CCWGG	5 cut(s)	294, 1602, 1849, 2847, 3072
PspFI	CCCAGC	1 cut(s)	346
PspGI	CCWGG	5 cut(s)	294, 1602, 1849, 2847, 3072
PspN4I	GGNNCC	6 cut(s)	1195, 1627, 1771, 1843, 2846, 3324
PspPI	GGNCC	8 cut(s)	156, 1390, 1553, 1697, 1841, 2063, 2285, 3025
PspPPI	RGGWCCY	1 cut(s)	1841
PstI	CTGCAG	3 cut(s)	441, 503, 2021
PstNI	CAGNNNCTG	1 cut(s)	1856
PsuI	RGATCY	4 cut(s)	998, 1193, 1816, 3322
PvuII	CAGCTG	3 cut(s)	341, 931, 1570
RsaI	GTAC	7 cut(s)	887, 2030, 2414, 2642, 2781, 2831, 3354
RsaNI	GTAC	7 cut(s)	886, 2029, 2413, 2641, 2780, 2830, 3353
RseI	CAYNNNNRTG	2 cut(s)	2868, 3033
SapI	GCTCTTC	1 cut(s)	3366
SatI	GCNGC	9 cut(s)	248, 648, 1289, 1679, 1921, 2017, 2626, 3251, 3377
Sau96I	GGNCC	8 cut(s)	156, 1390, 1553, 1697, 1841, 2063, 2285, 3025
SchI	GAGTC	4 cut(s)	14, 24, 2720, 2758
ScrFI	CCNGG	5 cut(s)	296, 1604, 1851, 2849, 3074
SduI	GDGCHC	2 cut(s)	694, 2636
SfaNI	GCATC	3 cut(s)	2179, 2333, 3107
SfcI	CTRYAG	5 cut(s)	437, 499, 2017, 2538, 2925
SinI	GGWCC	4 cut(s)	156, 1553, 1697, 1841
SmiMI	CAYNNNNRTG	2 cut(s)	2868, 3033
SmlI	CTYRAG	7 cut(s)	1187, 1670, 1724, 2552, 2629, 3380, 3452
SmoI	CTYRAG	7 cut(s)	1187, 1670, 1724, 2552, 2629, 3380, 3452
SpeI	ACTAGT	1 cut(s)	3271
SphI	GCATGC	1 cut(s)	1882
SseBI	AGGCCT	3 cut(s)	98, 291, 2816
SsiI	CCGC	4 cut(s)	524, 975, 1407, 2802
SspI	AATATT	1 cut(s)	2914
StuI	AGGCCT	3 cut(s)	98, 291, 2816
StyD4I	CCNGG	5 cut(s)	294, 1602, 1849, 2847, 3072
StyI	CCWWGG	8 cut(s)	274, 427, 633, 698, 1531, 1750, 3165, 3348
TaaI	ACNGT	4 cut(s)	58, 1769, 1813, 2638
TaiI	ACGT	2 cut(s)	2418, 3177
TaqI	TCGA	2 cut(s)	93, 1423
TatI	WGTACW	4 cut(s)	885, 2028, 2640, 2829
TscAI	CASTG	5 cut(s)	182, 2593, 2643, 2751, 2888
TseFI	GTSAC	1 cut(s)	200
TseI	GCWGC	9 cut(s)	247, 647, 1288, 1678, 1920, 2016, 2625, 3250, 3376
Tsp45I	GTSAC	1 cut(s)	200
TspGWI	ACGGA	1 cut(s)	2407
TspRI	CASTG	5 cut(s)	182, 2593, 2643, 2751, 2888
Van91I	CCANNNNNTGG	2 cut(s)	1499, 3274
Vha464I	CTTAAG	1 cut(s)	3452
VneI	GTGCAC	1 cut(s)	690
VpaK11BI	GGWCC	4 cut(s)	156, 1553, 1697, 1841
VspI	ATTAAT	1 cut(s)	1251
XagI	CCTNNNNNAGG	1 cut(s)	1187
XbaI	TCTAGA	1 cut(s)	1381
XceI	RCATGY	4 cut(s)	1762, 1882, 1945, 2379
XcmI	CCANNNNNNNNNTGG	2 cut(s)	640, 1384
XmaJI	CCTAGG	1 cut(s)	698
XmiI	GTMKAC	1 cut(s)	3171
XmnI	GAANNNNTTC	5 cut(s)	107, 283, 1435, 1460, 2437
Zsp2I	ATGCAT	2 cut(s)	2326, 2794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.