Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0008065

Physical Location & Seq

Genomic Coordinates Reverse (-)

9522603 .. 9524027

Gene Structure 1425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG103500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGCTCAATGGTTCCATTCCGAAAAGCATGGCTCACCTTAATCGGCTAACGACTCTGGATCTCTCTCACAATCATCTCTCAGGACCCATTCCAGGGTCTGTGATCTCAGGGATGAAAAGCATGCAGATATATTTGAATTTCTCATACAATTTCTTGGATGGCAGCATTCCAGACGAGCTTGGCATGTTGGAAATGGTGCAGGCCATTGACATTTCCAACAATAATCTTTCAGGGATGATTCCTGTAGCACTAGAAGGCTGCAGGAATTTGTACTCTCTTGATCTGTCAGGTAACAAACTTTCTGGGCCAATTCAAGCTGAAGTTTTTGCTAAGATGGACACCTTCACAAGCTTGAACCTTTCAAGGAATCACTTAGATGGTGAACTCCCAGAAAAGTTGACAAATCTGAAGCATCTTAGCTCCCTTGATCTTTCCCAGAATTTTCTGAGGGGAGTTATCCGTGAGAGCTTTACCAATTTTACCAGCTTGAAACACTTGAACCTCTCTTTCAATCAACTTGAAGGCCCTGTTCCAGACACCGGACTATTCAGAAGTATAAATGCATCCAGCTTGGTAGGAAACCCGGATCTCTGTGGAAACAAACTGCTCAAGACATGCAAGAAAAGTTCACACCGGATATCCAAGACAACCATGTATGTTCTCGTGGCGCTTGGAATAATTTCTCTACTGCTTATTTTAGTGATCATTTTCCTAATCCGCAGCCGGTTCACCAAAATGTGTAAGCCAGAAAAGATTGAAAATCCAGAACCAGAGTGTGCTGCAGCTTTGGCACTCAAGAGGTTTGATCAAAAGGATTTAGAAAATGCCACTAGTTTCTTTAGCAAAGAGAACATTTTAGGCACAAGCAGGTTGAGTACTGTGTACAAGGGTCAACTGGAAGATGAGCAGATAGTAGCCATAAAGAGATTGAATCTCCAGGAATTCTCAGTTGAGTCAGATAAATGCTTCAATAGAGAAATCAAGACACTGAGTCAACTAAGGCACAGGAATTTGGTGAAGGTACTTGGCTATGCATGGGAGAGCGGGAAATTAAAGGCCTTGGTTCTGGAATACATGGAGAATGGAAACCTGGAGAACATTATACATGAAGATGGGATGAATCAGTGGAGATTGACATTCTCTCAGAGAATCAATATTTTAATCTCTATAGCAAGTGGACTGGATTACCTGCATTCAGGATATGATTTTCCCATCGTTCATTGCGACTTGAAGCCTTCTAACATCTTGTTGGATGAGGATTGGGAGGCCCATGTGAGTGATTTTGGAACAGCTAGAATGTTGGGTGTTCATCTCCAGGATGGGAGTAGCCTTTCCACGGCATCAGCTTTCCAAGGCACAATTGGCTACTTAGCACCAGGTGACATTTTGCTGGCTATTCTCAATTATAAACTCATCTCCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006898 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010359 GO:0012505 GO:0016020 GO:0016043 GO:0016045 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031410 GO:0031982 GO:0032409 GO:0032412 GO:0032879 GO:0033036 GO:0033037 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0042545 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065009 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098542 GO:0098543 GO:0098581 GO:0098657 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

474

Amino Acids

52.75

Weight (kDa)

6.37

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	63 - 100	5.7e-06	Leucine rich repeat
LRR_8	PF13855	67 - 125	2.9e-08	Leucine rich repeat
LRR_8	PF13855	116 - 173	2.8e-08	Leucine rich repeat
Pkinase	PF00069	281 - 459	8.3e-32	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	283 - 434	7.7e-26	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46330 AT5G46330
fragaria_vesca	FvH4_3g06100
malus_domestica	MD05G1289000.v1.1
prunus_persica	Prupe.4G076500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0013311
rosa_laevigata	RLG00000032007 RLG00000032011
rosa_multiflora	Rmu_co8051088.1_g000001 Rmu_sc0003458.1_g000007 Rmu_sc0003458.1_g000011 Rmu_sc0004521.1_g000021 Rmu_sc0007948.1_g000008
rosa_roxburghii	Rroxscaffold_1G00063230
rosa_rugosa	Rorug05G0008800
rosa_samantha	Rh5AG103400 Rh5AG103500 Rh5AG104200 Rh5BG100200 Rh5CG111400 Rh5CG111800 Rh5DG099000
rosa_wichuraiana	Rw5G009030 Rw5G009070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1407
Acc36I	ACCTGC	2 cut(s)	858, 1197
AccBSI	CCGCTC	1 cut(s)	1044
AciI	CCGC	2 cut(s)	718, 1044
AclWI	GGATC	2 cut(s)	66, 594
AcsI	RAATTY	5 cut(s)	136, 265, 439, 941, 1009
AcuI	CTGAAG	2 cut(s)	339, 428
AdeI	CACNNNGTG	1 cut(s)	1379
AfaI	GTAC	4 cut(s)	272, 877, 884, 1023
AfiI	CCNNNNNNNGG	4 cut(s)	92, 93, 539, 1336
AhdI	GACNNNNNGTC	1 cut(s)	990
AhlI	ACTAGT	1 cut(s)	830
AjnI	CCWGG	5 cut(s)	91, 936, 1089, 1314, 1375
AlwI	GGATC	2 cut(s)	66, 594
AlwNI	CAGNNNCTG	1 cut(s)	98
AoxI	GGCC	5 cut(s)	201, 305, 523, 1056, 1266
ApeKI	GCWGC	5 cut(s)	162, 258, 720, 779, 782
ApoI	RAATTY	5 cut(s)	136, 265, 439, 941, 1009
Asp700I	GAANNNNTTC	1 cut(s)	679
AspLEI	GCGC	1 cut(s)	670
AspS9I	GGNCC	4 cut(s)	83, 305, 524, 1267
AsuC2I	CCSGG	1 cut(s)	584
AsuHPI	GGTGA	5 cut(s)	26, 392, 721, 1027, 1391
AvaII	GGWCC	1 cut(s)	83
BauI	CACGAG	1 cut(s)	662
BbvI	GCAGC	5 cut(s)	174, 245, 732, 766, 794
BccI	CCATC	6 cut(s)	152, 328, 371, 1106, 1220, 1313
BceAI	ACGGC	1 cut(s)	1353
BciT130I	CCWGG	5 cut(s)	93, 938, 1091, 1316, 1377
BclI	TGATCA	2 cut(s)	702, 805
BcnI	CCSGG	1 cut(s)	584
BcuI	ACTAGT	1 cut(s)	830
BfaI	CTAG	3 cut(s)	251, 831, 1293
BfmI	CTRYAG	4 cut(s)	243, 259, 780, 1167
BfoI	RGCGCY	1 cut(s)	671
BfuAI	ACCTGC	2 cut(s)	858, 1197
BisI	GCNGC	5 cut(s)	163, 259, 721, 780, 783
BlsI	GCNGC	5 cut(s)	164, 260, 722, 781, 784
BmcAI	AGTACT	1 cut(s)	877
Bme1390I	CCNGG	6 cut(s)	93, 584, 938, 1091, 1316, 1377
Bme18I	GGWCC	1 cut(s)	83
BmeRI	GACNNNNNGTC	1 cut(s)	990
BmgT120I	GGNCC	4 cut(s)	83, 305, 524, 1267
BmiI	GGNNCC	2 cut(s)	13, 85
BmrFI	CCNGG	6 cut(s)	93, 584, 938, 1091, 1316, 1377
BmsI	GCATC	3 cut(s)	421, 572, 1349
BpmI	CTGGAG	3 cut(s)	920, 1112, 1298
BpuEI	CTTGAG	2 cut(s)	593, 779
BpuMI	CCSGG	1 cut(s)	584
BsaJI	CCNNGG	4 cut(s)	92, 1059, 1335, 1351
BsaWI	WCCGGW	2 cut(s)	539, 633
Bsc4I	CCNNNNNNNGG	4 cut(s)	92, 93, 539, 1336
Bse118I	RCCGGY	1 cut(s)	723
Bse1I	ACTGG	2 cut(s)	900, 1185
Bse3DI	GCAATG	1 cut(s)	1219
BseBI	CCWGG	5 cut(s)	93, 938, 1091, 1316, 1377
BseDI	CCNNGG	4 cut(s)	92, 1059, 1335, 1351
BseGI	GGATG	7 cut(s)	117, 163, 240, 563, 1122, 1258, 1324
BseLI	CCNNNNNNNGG	4 cut(s)	92, 93, 539, 1336
BseMI	GCAATG	1 cut(s)	1219
BseMII	CTCAG	6 cut(s)	93, 120, 437, 960, 980, 1157
BseNI	ACTGG	2 cut(s)	900, 1185
BseXI	GCAGC	5 cut(s)	174, 245, 732, 766, 794
BsgI	GTGCAG	1 cut(s)	218
BshFI	GGCC	5 cut(s)	203, 307, 525, 1058, 1268
BsiSI	CCGG	4 cut(s)	540, 584, 634, 724
BslI	CCNNNNNNNGG	4 cut(s)	92, 93, 539, 1336
BsmI	GAATGC	2 cut(s)	165, 1192
BsnI	GGCC	5 cut(s)	203, 307, 525, 1058, 1268
Bsp1407I	TGTACA	1 cut(s)	882
Bsp143I	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
BspACI	CCGC	2 cut(s)	718, 1044
BspANI	GGCC	5 cut(s)	203, 307, 525, 1058, 1268
BspCNI	CTCAG	6 cut(s)	92, 119, 438, 959, 981, 1156
BspLI	GGNNCC	2 cut(s)	13, 85
BspMAI	CTGCAG	2 cut(s)	263, 784
BspMI	ACCTGC	2 cut(s)	858, 1197
BspPI	GGATC	2 cut(s)	66, 594
BsrBI	CCGCTC	1 cut(s)	1044
BsrDI	GCAATG	1 cut(s)	1219
BsrFI	RCCGGY	1 cut(s)	723
BsrGI	TGTACA	1 cut(s)	882
BsrI	ACTGG	2 cut(s)	900, 1185
BssAI	RCCGGY	1 cut(s)	723
BssECI	CCNNGG	4 cut(s)	92, 1059, 1335, 1351
BssMI	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
BssSI	CACGAG	1 cut(s)	662
BssT1I	CCWWGG	2 cut(s)	1059, 1351
Bst2BI	CACGAG	1 cut(s)	662
Bst2UI	CCWGG	5 cut(s)	93, 938, 1091, 1316, 1377
Bst4CI	ACNGT	1 cut(s)	880
BstAUI	TGTACA	1 cut(s)	882
BstC8I	GCNNGC	3 cut(s)	122, 201, 1392
BstDSI	CCRYGG	1 cut(s)	1335
BstF5I	GGATG	7 cut(s)	117, 163, 240, 563, 1122, 1258, 1324
BstH2I	RGCGCY	1 cut(s)	671
BstHHI	GCGC	1 cut(s)	670
BstKTI	GATC	7 cut(s)	61, 105, 283, 430, 589, 705, 808
BstMBI	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
BstMWI	GCNNNNNNNGC	2 cut(s)	788, 1362
BstNI	CCWGG	5 cut(s)	93, 938, 1091, 1316, 1377
BstNSI	RCATGY	3 cut(s)	124, 187, 618
BstSCI	CCNGG	6 cut(s)	91, 582, 936, 1089, 1314, 1375
BstSFI	CTRYAG	4 cut(s)	243, 259, 780, 1167
BstV1I	GCAGC	5 cut(s)	174, 245, 732, 766, 794
BstX2I	RGATCY	2 cut(s)	58, 586
BstYI	RGATCY	2 cut(s)	58, 586
BsuRI	GGCC	5 cut(s)	203, 307, 525, 1058, 1268
BtgI	CCRYGG	1 cut(s)	1335
BtsCI	GGATG	7 cut(s)	117, 163, 240, 563, 1122, 1258, 1324
BtsIMutI	CAGTG	2 cut(s)	986, 1130
BveI	ACCTGC	2 cut(s)	858, 1197
Cac8I	GCNNGC	3 cut(s)	122, 201, 1392
CaiI	CAGNNNCTG	1 cut(s)	98
CfoI	GCGC	1 cut(s)	670
Cfr10I	RCCGGY	1 cut(s)	723
Cfr13I	GGNCC	4 cut(s)	83, 305, 524, 1267
CsiI	ACCWGGT	1 cut(s)	1375
Csp6I	GTAC	4 cut(s)	271, 876, 883, 1022
CviAII	CATG	9 cut(s)	28, 121, 184, 615, 652, 1035, 1075, 1106, 1271
CviQI	GTAC	4 cut(s)	271, 876, 883, 1022
DpnI	GATC	7 cut(s)	60, 104, 282, 429, 588, 704, 807
DpnII	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
DraIII	CACNNNGTG	1 cut(s)	1379
DriI	GACNNNNNGTC	1 cut(s)	990
Eam1105I	GACNNNNNGTC	1 cut(s)	990
Eco130I	CCWWGG	2 cut(s)	1059, 1351
Eco147I	AGGCCT	1 cut(s)	1058
Eco32I	GATATC	1 cut(s)	639
Eco47I	GGWCC	1 cut(s)	83
Eco57I	CTGAAG	2 cut(s)	339, 428
EcoO109I	RGGNCCY	2 cut(s)	83, 524
EcoRI	GAATTC	1 cut(s)	941
EcoRII	CCWGG	5 cut(s)	91, 936, 1089, 1314, 1375
EcoRV	GATATC	1 cut(s)	639
EcoT14I	CCWWGG	2 cut(s)	1059, 1351
EcoT22I	ATGCAT	2 cut(s)	565, 1036
ErhI	CCWWGG	2 cut(s)	1059, 1351
FaeI	CATG	9 cut(s)	31, 124, 187, 618, 655, 1038, 1078, 1109, 1274
FatI	CATG	9 cut(s)	27, 120, 183, 614, 651, 1034, 1074, 1105, 1270
FauI	CCCGC	1 cut(s)	1037
FbaI	TGATCA	2 cut(s)	702, 805
Fnu4HI	GCNGC	5 cut(s)	163, 259, 721, 780, 783
FokI	GGATG	7 cut(s)	124, 170, 247, 550, 1129, 1265, 1331
Fsp4HI	GCNGC	5 cut(s)	163, 259, 721, 780, 783
FspBI	CTAG	3 cut(s)	251, 831, 1293
GlaI	GCGC	1 cut(s)	669
GluI	GCNGC	5 cut(s)	163, 259, 721, 780, 783
GsuI	CTGGAG	3 cut(s)	920, 1112, 1298
HaeII	RGCGCY	1 cut(s)	671
HaeIII	GGCC	5 cut(s)	203, 307, 525, 1058, 1268
HapII	CCGG	4 cut(s)	540, 584, 634, 724
HhaI	GCGC	1 cut(s)	670
Hin1II	CATG	9 cut(s)	31, 124, 187, 618, 655, 1038, 1078, 1109, 1274
Hin6I	GCGC	1 cut(s)	668
HinP1I	GCGC	1 cut(s)	668
HincII	GTYRAC	3 cut(s)	399, 893, 995
HindII	GTYRAC	3 cut(s)	399, 893, 995
HindIII	AAGCTT	1 cut(s)	349
HinfI	GANTC	8 cut(s)	52, 238, 367, 931, 953, 991, 1120, 1149
HpaII	CCGG	4 cut(s)	540, 584, 634, 724
HphI	GGTGA	5 cut(s)	26, 392, 721, 1027, 1391
Hpy166II	GTNNAC	8 cut(s)	383, 399, 629, 729, 883, 893, 995, 1178
Hpy188I	TCNGA	6 cut(s)	21, 408, 447, 551, 958, 1146
Hpy8I	GTNNAC	8 cut(s)	383, 399, 629, 729, 883, 893, 995, 1178
HpyAV	CCTTC	5 cut(s)	248, 352, 515, 1012, 1245
HpyCH4III	ACNGT	1 cut(s)	880
HpyCH4V	TGCA	8 cut(s)	124, 199, 261, 563, 618, 782, 1034, 1192
HpyF10VI	GCNNNNNNNGC	2 cut(s)	788, 1362
Hsp92II	CATG	9 cut(s)	31, 124, 187, 618, 655, 1038, 1078, 1109, 1274
HspAI	GCGC	1 cut(s)	668
Ksp22I	TGATCA	2 cut(s)	702, 805
Kzo9I	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
LmnI	GCTCC	1 cut(s)	425
Lsp1109I	GCAGC	5 cut(s)	174, 245, 732, 766, 794
LweI	GCATC	3 cut(s)	421, 572, 1349
MabI	ACCWGGT	1 cut(s)	1375
MaeI	CTAG	3 cut(s)	251, 831, 1293
MaeIII	GTNAC	2 cut(s)	290, 1379
MalI	GATC	7 cut(s)	60, 104, 282, 429, 588, 704, 807
MbiI	CCGCTC	1 cut(s)	1044
MboI	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
MboII	GAAGA	2 cut(s)	911, 1121
MfeI	CAATTG	1 cut(s)	1359
MflI	RGATCY	2 cut(s)	58, 586
MlyI	GAGTC	3 cut(s)	46, 962, 1000
MmeI	TCCRAC	3 cut(s)	168, 240, 1230
MnlI	CCTC	5 cut(s)	441, 512, 792, 1249, 1258
Mph1103I	ATGCAT	2 cut(s)	565, 1036
MroXI	GAANNNNTTC	1 cut(s)	679
MseI	TTAA	3 cut(s)	39, 1052, 1160
MslI	CAYNNNNRTG	4 cut(s)	375, 734, 1110, 1275
MspI	CCGG	4 cut(s)	540, 584, 634, 724
MspR9I	CCNGG	6 cut(s)	93, 584, 938, 1091, 1316, 1377
MunI	CAATTG	1 cut(s)	1359
Mva1269I	GAATGC	2 cut(s)	165, 1192
MvaI	CCWGG	5 cut(s)	93, 938, 1091, 1316, 1377
MwoI	GCNNNNNNNGC	2 cut(s)	788, 1362
NciI	CCSGG	1 cut(s)	584
NdeII	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
NlaIII	CATG	9 cut(s)	31, 124, 187, 618, 655, 1038, 1078, 1109, 1274
NlaIV	GGNNCC	2 cut(s)	13, 85
NmuCI	GTSAC	1 cut(s)	1379
NsiI	ATGCAT	2 cut(s)	565, 1036
NspI	RCATGY	3 cut(s)	124, 187, 618
PaeI	GCATGC	1 cut(s)	124
PceI	AGGCCT	1 cut(s)	1058
PcsI	WCGNNNNNNNCGW	1 cut(s)	1221
PctI	GAATGC	2 cut(s)	165, 1192
PdmI	GAANNNNTTC	1 cut(s)	679
PfeI	GAWTC	5 cut(s)	238, 367, 931, 1120, 1149
PfoI	TCCNGGA	2 cut(s)	936, 1314
PkrI	GCNGC	5 cut(s)	164, 260, 722, 781, 784
PleI	GAGTC	3 cut(s)	46, 961, 999
PpsI	GAGTC	3 cut(s)	46, 961, 999
PpuMI	RGGWCCY	1 cut(s)	83
PsiI	TTATAA	1 cut(s)	1407
Psp5II	RGGWCCY	1 cut(s)	83
Psp6I	CCWGG	5 cut(s)	91, 936, 1089, 1314, 1375
PspGI	CCWGG	5 cut(s)	91, 936, 1089, 1314, 1375
PspN4I	GGNNCC	2 cut(s)	13, 85
PspPI	GGNCC	4 cut(s)	83, 305, 524, 1267
PspPPI	RGGWCCY	1 cut(s)	83
PstI	CTGCAG	2 cut(s)	263, 784
PstNI	CAGNNNCTG	1 cut(s)	98
PsuI	RGATCY	2 cut(s)	58, 586
RsaI	GTAC	4 cut(s)	272, 877, 884, 1023
RsaNI	GTAC	4 cut(s)	271, 876, 883, 1022
RseI	CAYNNNNRTG	4 cut(s)	375, 734, 1110, 1275
SaqAI	TTAA	3 cut(s)	39, 1052, 1160
SatI	GCNGC	5 cut(s)	163, 259, 721, 780, 783
Sau3AI	GATC	7 cut(s)	58, 102, 280, 427, 586, 702, 805
Sau96I	GGNCC	4 cut(s)	83, 305, 524, 1267
ScaI	AGTACT	1 cut(s)	877
SchI	GAGTC	3 cut(s)	46, 962, 1000
ScrFI	CCNGG	6 cut(s)	93, 584, 938, 1091, 1316, 1377
SexAI	ACCWGGT	1 cut(s)	1375
SfaNI	GCATC	3 cut(s)	421, 572, 1349
SfcI	CTRYAG	4 cut(s)	243, 259, 780, 1167
SinI	GGWCC	1 cut(s)	83
SmiMI	CAYNNNNRTG	4 cut(s)	375, 734, 1110, 1275
SmlI	CTYRAG	2 cut(s)	608, 794
SmoI	CTYRAG	2 cut(s)	608, 794
SpeI	ACTAGT	1 cut(s)	830
SphI	GCATGC	1 cut(s)	124
SseBI	AGGCCT	1 cut(s)	1058
SsiI	CCGC	2 cut(s)	718, 1044
SspI	AATATT	1 cut(s)	1156
SspMI	CTAG	3 cut(s)	251, 831, 1293
StuI	AGGCCT	1 cut(s)	1058
StyD4I	CCNGG	6 cut(s)	91, 582, 936, 1089, 1314, 1375
StyI	CCWWGG	2 cut(s)	1059, 1351
TaaI	ACNGT	1 cut(s)	880
TatI	WGTACW	3 cut(s)	270, 875, 882
TfiI	GAWTC	5 cut(s)	238, 367, 931, 1120, 1149
Tru1I	TTAA	3 cut(s)	39, 1052, 1160
Tru9I	TTAA	3 cut(s)	39, 1052, 1160
TscAI	CASTG	2 cut(s)	993, 1130
TseFI	GTSAC	1 cut(s)	1379
TseI	GCWGC	5 cut(s)	162, 258, 720, 779, 782
Tsp45I	GTSAC	1 cut(s)	1379
TspDTI	ATGAA	5 cut(s)	128, 1122, 1133, 1208, 1298
TspGWI	ACGGA	1 cut(s)	449
TspRI	CASTG	2 cut(s)	993, 1130
VpaK11BI	GGWCC	1 cut(s)	83
XapI	RAATTY	5 cut(s)	136, 265, 439, 941, 1009
XceI	RCATGY	3 cut(s)	124, 187, 618
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmnI	GAANNNNTTC	1 cut(s)	679
XspI	CTAG	3 cut(s)	251, 831, 1293
ZrmI	AGTACT	1 cut(s)	877
Zsp2I	ATGCAT	2 cut(s)	565, 1036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.