Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008065

Physical Location & Seq

Genomic Coordinates Reverse (-)

9745468 .. 9768259

Gene Structure 22792 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G009070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3477 bp

ATGGAGTCTCATAGTGTCAGTTTGGTCATAGTCATAGTCTGTTCTGTTCTACTTGCTGGTCTGTCTGCTCAGCCAAGCTTGGAGGTTGAAGTCGAGGCCTTGAAGGCATTCAAGAAATCCATCACCAGCGACCCGAATGGAACACTTGCAGATTGGACGTCAGACGCAAGCCATCACTGCAATTGGTCTGGCATTGCCTGTGACCCCTCAACAAATCAAGTCATTTCAATTTCTCTGGCGGAAAAGCAGCTCACAGGCGTGATCTCACCCTTCCTCGGAAACATTTCAGGCCTCCAGGTTCTTGATCTAACTTCAAACTCATTCACTGGACATATTCCAGCTGAGCTGGGACTTTGCTCCCAACTTTCTCTACTTGCTCTGTATCAAAATTCTCTTTCGGGTTCAATTCCACCAGAACTAGGAAACCTTGGTAATCTGCAGTTATTAGATTTGGGTGATAACCTTTTGAGTGGAAGCATGCCGGAGAGCATTTGCAACTGTAGAAACTTGTCTTTGTTTGGTGCGGAATTTAATAACCTCACTGGCAAACTCCCTTCAAACATAGGAAATCTTGTTAATCTTGAGCTTCTTATAGCAAGTGGCAACAACTTTGTAGGCTCATTACCTGCTTCCATGGGTAAGTTGGCTGCTTTCAAAGAGGTTGATTTAAGTAAAAACCAGTTGTCCGGTGCAATACCTAAGGAGCTAGGGAATCTGTCAAATTTAGAACAACTGGTAGTGTTTGATAATTCCTTTGTTGGGGAAATCCCTTCTGAACTAAGTCGGTGTAAGAAGCTTGTTAACCTTGAACTTTACGAGAATCATTTCACCGGAAGCATTCCCTCTGAGCTTGGAAGTTTGGTTCATTTAGAAACATTGAGATTGTACAAGAATAGGCTAAACTCTACAATTCCACTCTCCATTTTTCAGCTGAAATCATTAACCCATTTAGGACTTTCAGAAAATGAGTTAACTGGGACAATACCATCCGAGCTTGGATCTTTAAGATCATTGCAAGTATTGACCCTGCACTCCAATAAGTTTACCGGAGAGATCCCCTCATCCTTGACAAGCTTGACAAATCTAACATATCTGTCAATGAGCTTAAATTCTCTAACAGGGGAACTTCCATCAAACATAGGGTTGCTTTATAATCTTAAAAACCTCTCCATGAATGGCAACCTTCTTGAGGGATCCATACCATCTAGTATCACCAACTGTACCAATCTTCAAGTTATAAGCTTGGCTTACAATAGAATAACTGGAAAAATACCTCAGGGTCTGGGGCAGTTGCTGAAGCTTAATTTCCTTTCTGTTGGATCCAATAAATTGTTCGGAGAAATCCCAGATGACCTCTTCAATTGCACTAGTCTTCTCACTCTAGATTTGGGCCTGAACAACTTTAGTGGTTATCTGAAACCCGGATTTGGAAAACTTTCCAATCTCAGGGTTTTGAGAGCCACCTCCAATTCATTTGTCGGACAAATTCCACCAGAGATTGGCCAACTAAACCAACTTATCATCCTCACACTTGCTGAGAATAGATTCTCAGGTCCAGTTCCACCACAGCTGTCTAATCTCTCTCACCTACAAGGGCTATCCCTGCACGACAATGCTTTAGAAGGAGCCATCCCTGAGAAACTTTTTGAGCTAAAAGAGCTAACTGTTCTAGAGTTGCAGCAAAACAAACTGACCGGTCCAATTCCAGATACTGTCTCAAAACTTGAGTTGCTTTCATATTTAAACCTCCAAGGTAATATGCTCAATGGTTCCATTCCGAAAAGCATGGCTCACCTTAATCGGCTAACGACTCTGGATCTCTCTCACAATCATCTCTCAGGACCCATTCCAGGGTCTGTGATCTCAGGGATGAAAAGCATGCAGATATATTTGAATTTCTCATACAATTTCTTGGATGGCAGCATTCCAGACGAGCTTGGCATGTTGGAAATGGTGCAGGCCATTGACATTTCCAACAATAATCTTTCAGGGATGATTCCTGTAGCACTAGAAGGCTGCAGGAATTTGTACTCTCTTGATCTGTCAGGTAACAAACTTTCTGGGCCAATTCAAGCTGAAGTTTTTGCTAAGATGGACACCTTCACAAGCTTGAACCTTTCAAGGAATCACTTAGATGGTGAACTCCCAGAAAAGTTGACAAATCTGAAGCATCTTAGCTCCCTTGATCTTTCCCAGAATTTTCTGAGGGGAGTTATCCGTGAGAGCTTTACCAATTTTACCAGCTTGAAACACTTGAACCTCTCTTTCAATCAACTTGAAGGCCCTGTTCCAGACACCGGACTATTCAGAAGTATAAATGCATCCAGCTTGGTAGGAAACCCGGATCTCTGTGGAAACAAACTGCTCAAGACATGCAAGAAAAGTTCACACCGGATATCCAAGACAACCATGTATGTTCTCGTGGCGCTTGGAATAATTTCTCTACTGCTTATTTTAGTGATCATTTTCCTAATCCGCAGCCGGTTCACCAAAATGTGTAAGCCAGAAAAGATTGAAAATCCAGAACCAGAGTGTGCTGCAGCTTTGGCACTCAAGAGGTTTGATCAAAAGGATTTAGAAAATGCCACTACTTTCTTTAGCAAAGAGAACATTTTAGGCACAAGCAGGTTGAGTACTGTGTACAAGGGTCAACTGGAAGATGAGCAGATAGTAGCCATAAAGAGATTGAATCTCCAGGAATTCTCAGTTGAGTCAGATAAATGCTTCAATAGAGAAATCAAGACACTGAGTCAACTAAGGCACAGGAATTTGGTGAAGGTACTTGGCTATGCATGGGAGAGCGGGAAATTAAAGGCCTTGGTTCTGGAATACATGGAGAATGGAAACCTGGAGAACATTATACATGAAGATGGGATGAATCAGTGGAGATTGACATTCTCTCAGAGAATCAATATTTTAATCTCTATAGCAAGTGGACTGGATTACCTGCATTCAGGATATGATTTTCCCATCGTTCATTGCGACTTGAAGCCTTCTAACATCTTGTTGGATGAGGATTGGGAGGCCCATGTGAGTGATTTTGGAACAGCTAGAATGTTGGGTGTTCATCTCCAGGATGGGAGTAGCCTTTCCACGGCATCAGCTTTCCAAGGCACAATTGGCTACTTAGCACCAGAGTTTGCATACATGTGGAAAATCACAACCAAGGTAGACGTGTTCAGCTTTGGTGTAATATTGATGGAGTTACTGACAAGACAAAGGCCAACAGGACTCATGGAAGAGAATGGGCTGCCAATGAGCTTGCACCAACTAGTGGAGGAAGCCCTTGCAAATGGATCAGATTCGCTTCTTGAAGTTCTGGACCCATTGCTAACTTCAAATATCTCCAAGGAACAGGAGGCAAGTGCAGAAGAGCTGCTCAAGCTAGCCTTGTTTTGCACCAACCAAAATCCCGAGGAGCGACCTATCGTGAATGAGGTGTTGTCTGCTCTTTTGAAGCTGAAAAAGGCAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006898 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010359 GO:0012505 GO:0016020 GO:0016043 GO:0016045 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031410 GO:0031982 GO:0032409 GO:0032412 GO:0032879 GO:0033036 GO:0033037 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0042545 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065009 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098542 GO:0098543 GO:0098581 GO:0098657 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1158

Amino Acids

126.57

Weight (kDa)

5.48

Isoelectric Point (pI)

29.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 68	1.9e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	182 - 272	8.3e-07	Leucine-rich repeat region
LRR_14	PF23598	267 - 371	8.7e-11	Leucine-rich repeat region
LRR_8	PF13855	289 - 348	6.3e-07	Leucine rich repeat
LRR_14	PF23598	378 - 488	2.2e-07	Leucine-rich repeat region
LRR_14	PF23598	472 - 561	3.1e-07	Leucine-rich repeat region
LRR_8	PF13855	553 - 612	5.5e-09	Leucine rich repeat
LRR_8	PF13855	653 - 711	8.3e-08	Leucine rich repeat
LRR_8	PF13855	702 - 759	7.7e-08	Leucine rich repeat
Pkinase	PF00069	867 - 1149	4.6e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	869 - 1150	5.4e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46330 AT5G46330
fragaria_vesca	FvH4_3g06100
malus_domestica	MD05G1289000.v1.1
prunus_persica	Prupe.4G076500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0013311
rosa_laevigata	RLG00000032007 RLG00000032011
rosa_multiflora	Rmu_co8051088.1_g000001 Rmu_sc0003458.1_g000007 Rmu_sc0003458.1_g000011 Rmu_sc0004521.1_g000021 Rmu_sc0007948.1_g000008
rosa_roxburghii	Rroxscaffold_1G00063230
rosa_rugosa	Rorug05G0008800
rosa_samantha	Rh5AG103400 Rh5AG103500 Rh5AG104200 Rh5BG100200 Rh5CG111400 Rh5CG111800 Rh5DG099000
rosa_wichuraiana	Rw5G009030 Rw5G009070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1152, 1238
AatII	GACGTC	1 cut(s)	161
Acc36I	ACCTGC	3 cut(s)	634, 2616, 2955
AccB7I	CCANNNNNTGG	2 cut(s)	1499, 3274
AccBSI	CCGCTC	1 cut(s)	2802
AccI	GTMKAC	1 cut(s)	3171
AciI	CCGC	4 cut(s)	239, 524, 2476, 2802
AclWI	GGATC	9 cut(s)	1006, 1048, 1188, 1201, 1314, 1327, 1824, 2352, 3304
AcoI	YGGCCR	1 cut(s)	1501
AcuI	CTGAAG	3 cut(s)	1316, 2097, 2186
AcyI	GRCGYC	1 cut(s)	158
AfaI	GTAC	6 cut(s)	887, 1222, 2030, 2635, 2642, 2781
AflIII	ACRYGT	2 cut(s)	3147, 3174
AgeI	ACCGGT	1 cut(s)	1694
AhdI	GACNNNNNGTC	1 cut(s)	2748
AhlI	ACTAGT	2 cut(s)	1367, 3271
AjiI	CACGTC	1 cut(s)	3175
AjnI	CCWGG	5 cut(s)	294, 1849, 2694, 2847, 3072
AjuI	GAANNNNNNNTTGG	2 cut(s)	1316, 1348
AleI	CACNNNNGTG	1 cut(s)	257
AloI	GAACNNNNNNTCC	2 cut(s)	846, 878
Alw26I	GTCTC	2 cut(s)	12, 1720
AlwI	GGATC	9 cut(s)	1006, 1048, 1188, 1201, 1314, 1327, 1824, 2352, 3304
AlwNI	CAGNNNCTG	4 cut(s)	1282, 1294, 1712, 1856
Ama87I	CYCGRG	1 cut(s)	3413
AsiGI	ACCGGT	1 cut(s)	1694
Asp700I	GAANNNNTTC	6 cut(s)	107, 283, 824, 1435, 1544, 2437
AspLEI	GCGC	1 cut(s)	2428
AspS9I	GGNCC	8 cut(s)	1390, 1553, 1697, 1841, 2063, 2282, 3025, 3322
AsuC2I	CCSGG	2 cut(s)	1422, 2342
AsuNHI	GCTAGC	1 cut(s)	3385
AvaI	CYCGRG	1 cut(s)	3413
AvaII	GGWCC	4 cut(s)	1553, 1697, 1841, 3322
AxyI	CCTNAGG	2 cut(s)	699, 1275
BalI	TGGCCA	1 cut(s)	1503
BamHI	GGATCC	2 cut(s)	1193, 1319
BauI	CACGAG	1 cut(s)	2420
BbsI	GAAGAC	1 cut(s)	1364
BceAI	ACGGC	1 cut(s)	3111
BciT130I	CCWGG	5 cut(s)	296, 1851, 2696, 2849, 3074
BclI	TGATCA	2 cut(s)	2460, 2563
BcnI	CCSGG	2 cut(s)	1422, 2342
BcoDI	GTCTC	2 cut(s)	12, 1720
BcuI	ACTAGT	2 cut(s)	1367, 3271
BfmI	CTRYAG	6 cut(s)	437, 499, 2001, 2017, 2538, 2925
BfoI	RGCGCY	1 cut(s)	2429
BfuAI	ACCTGC	3 cut(s)	634, 2616, 2955
BglI	GCCNNNNNGGC	1 cut(s)	104
BlpI	GCTNAGC	2 cut(s)	69, 342
BmcAI	AGTACT	1 cut(s)	2635
Bme1390I	CCNGG	7 cut(s)	296, 1422, 1851, 2342, 2696, 2849, 3074
Bme18I	GGWCC	4 cut(s)	1553, 1697, 1841, 3322
BmeRI	GACNNNNNGTC	1 cut(s)	2748
BmeT110I	CYCGRG	1 cut(s)	3413
BmgBI	CACGTC	1 cut(s)	3175
BmgT120I	GGNCC	8 cut(s)	1390, 1553, 1697, 1841, 2063, 2282, 3025, 3322
BmiI	GGNNCC	6 cut(s)	1195, 1321, 1627, 1771, 1843, 3324
BmrFI	CCNGG	7 cut(s)	296, 1422, 1851, 2342, 2696, 2849, 3074
BmrI	ACTGGG	1 cut(s)	984
BmsI	GCATC	3 cut(s)	2179, 2330, 3107
BmtI	GCTAGC	1 cut(s)	3389
BmuI	ACTGGG	1 cut(s)	984
BpiI	GAAGAC	1 cut(s)	1364
BplI	GAGNNNNNCTC	2 cut(s)	1043, 1075
BpmI	CTGGAG	4 cut(s)	278, 2678, 2870, 3056
Bpu1102I	GCTNAGC	2 cut(s)	69, 342
BpuEI	CTTGAG	6 cut(s)	602, 1208, 1745, 2351, 2537, 3365
BpuMI	CCSGG	2 cut(s)	1422, 2342
BsaHI	GRCGYC	1 cut(s)	158
BsaWI	WCCGGW	6 cut(s)	686, 830, 1046, 1694, 2297, 2391
Bse118I	RCCGGY	2 cut(s)	1694, 2481
Bse1I	ACTGG	9 cut(s)	331, 547, 679, 738, 979, 1267, 1556, 2658, 2943
Bse21I	CCTNAGG	2 cut(s)	699, 1275
Bse3DI	GCAATG	4 cut(s)	192, 1010, 2977, 3326
BseBI	CCWGG	5 cut(s)	296, 1851, 2696, 2849, 3074
BseMI	GCAATG	4 cut(s)	192, 1010, 2977, 3326
BseNI	ACTGG	9 cut(s)	331, 547, 679, 738, 979, 1267, 1556, 2658, 2943
BseRI	GAGGAG	1 cut(s)	3431
BseYI	CCCAGC	1 cut(s)	346
BsgI	GTGCAG	4 cut(s)	1013, 1589, 1976, 3387
BshTI	ACCGGT	1 cut(s)	1694
BsiHKCI	CYCGRG	1 cut(s)	3413
BslFI	GGGAC	2 cut(s)	363, 991
BsmAI	GTCTC	2 cut(s)	12, 1720
BsmFI	GGGAC	2 cut(s)	363, 991
BsmI	GAATGC	4 cut(s)	107, 837, 1923, 2950
BsoBI	CYCGRG	1 cut(s)	3413
Bsp1407I	TGTACA	2 cut(s)	885, 2640
Bsp1720I	GCTNAGC	2 cut(s)	69, 342
Bsp19I	CCATGG	1 cut(s)	633
BspACI	CCGC	4 cut(s)	239, 524, 2476, 2802
BspLI	GGNNCC	6 cut(s)	1195, 1321, 1627, 1771, 1843, 3324
BspMAI	CTGCAG	3 cut(s)	441, 2021, 2542
BspMI	ACCTGC	3 cut(s)	634, 2616, 2955
BspOI	GCTAGC	1 cut(s)	3389
BspPI	GGATC	9 cut(s)	1006, 1048, 1188, 1201, 1314, 1327, 1824, 2352, 3304
BspQI	GCTCTTC	1 cut(s)	3366
BsrBI	CCGCTC	1 cut(s)	2802
BsrDI	GCAATG	4 cut(s)	192, 1010, 2977, 3326
BsrFI	RCCGGY	2 cut(s)	1694, 2481
BsrGI	TGTACA	2 cut(s)	885, 2640
BsrI	ACTGG	9 cut(s)	331, 547, 679, 738, 979, 1267, 1556, 2658, 2943
BssAI	RCCGGY	2 cut(s)	1694, 2481
BssNI	GRCGYC	1 cut(s)	158
BssSI	CACGAG	1 cut(s)	2420
BssT1I	CCWWGG	7 cut(s)	427, 633, 1750, 2817, 3109, 3165, 3348
Bst2BI	CACGAG	1 cut(s)	2420
Bst2UI	CCWGG	5 cut(s)	296, 1851, 2696, 2849, 3074
Bst4CI	ACNGT	5 cut(s)	500, 1220, 1666, 1714, 2638
Bst6I	CTCTTC	3 cut(s)	1361, 3234, 3366
BstACI	GRCGYC	1 cut(s)	158
BstAUI	TGTACA	2 cut(s)	885, 2640
BstC8I	GCNNGC	6 cut(s)	169, 479, 1880, 1959, 3263, 3387
BstDSI	CCRYGG	2 cut(s)	633, 3093
BstENI	CCTNNNNNAGG	1 cut(s)	1187
BstH2I	RGCGCY	1 cut(s)	2429
BstHHI	GCGC	1 cut(s)	2428
BstMAI	GTCTC	2 cut(s)	12, 1720
BstMWI	GCNNNNNNNGC	7 cut(s)	104, 177, 1603, 1657, 2546, 3120, 3382
BstNI	CCWGG	5 cut(s)	296, 1851, 2696, 2849, 3074
BstNSI	RCATGY	5 cut(s)	481, 1882, 1945, 2376, 3151
BstSCI	CCNGG	7 cut(s)	294, 1420, 1849, 2340, 2694, 2847, 3072
BstSFI	CTRYAG	6 cut(s)	437, 499, 2001, 2017, 2538, 2925
BstV2I	GAAGAC	1 cut(s)	1364
BstX2I	RGATCY	6 cut(s)	998, 1053, 1193, 1319, 1816, 2344
BstYI	RGATCY	6 cut(s)	998, 1053, 1193, 1319, 1816, 2344
Bsu36I	CCTNAGG	2 cut(s)	699, 1275
BtgI	CCRYGG	2 cut(s)	633, 3093
BtrI	CACGTC	1 cut(s)	3175
BtsI	GCAGTG	1 cut(s)	175
BtsIMutI	CAGTG	5 cut(s)	175, 324, 540, 2744, 2888
BveI	ACCTGC	3 cut(s)	634, 2616, 2955
Cac8I	GCNNGC	6 cut(s)	169, 479, 1880, 1959, 3263, 3387
CaiI	CAGNNNCTG	4 cut(s)	1282, 1294, 1712, 1856
CfoI	GCGC	1 cut(s)	2428
Cfr10I	RCCGGY	2 cut(s)	1694, 2481
Cfr13I	GGNCC	8 cut(s)	1390, 1553, 1697, 1841, 2063, 2282, 3025, 3322
CseI	GACGC	1 cut(s)	173
Csp6I	GTAC	6 cut(s)	886, 1221, 2029, 2634, 2641, 2780
CspAI	ACCGGT	1 cut(s)	1694
CspCI	CAANNNNNGTGG	2 cut(s)	1387, 1422
CviQI	GTAC	6 cut(s)	886, 1221, 2029, 2634, 2641, 2780
DraI	TTTAAA	1 cut(s)	1743
DriI	GACNNNNNGTC	1 cut(s)	2748
EaeI	YGGCCR	1 cut(s)	1501
Eam1104I	CTCTTC	3 cut(s)	1361, 3234, 3366
Eam1105I	GACNNNNNGTC	1 cut(s)	2748
EarI	CTCTTC	3 cut(s)	1361, 3234, 3366
EciI	GGCGGA	1 cut(s)	254
Eco130I	CCWWGG	7 cut(s)	427, 633, 1750, 2817, 3109, 3165, 3348
Eco147I	AGGCCT	3 cut(s)	98, 291, 2816
Eco32I	GATATC	1 cut(s)	2397
Eco47I	GGWCC	4 cut(s)	1553, 1697, 1841, 3322
Eco57I	CTGAAG	3 cut(s)	1316, 2097, 2186
Eco81I	CCTNAGG	2 cut(s)	699, 1275
Eco88I	CYCGRG	1 cut(s)	3413
EcoNI	CCTNNNNNAGG	1 cut(s)	1187
EcoO109I	RGGNCCY	2 cut(s)	1841, 2282
EcoRI	GAATTC	1 cut(s)	2699
EcoRII	CCWGG	5 cut(s)	294, 1849, 2694, 2847, 3072
EcoRV	GATATC	1 cut(s)	2397
EcoT14I	CCWWGG	7 cut(s)	427, 633, 1750, 2817, 3109, 3165, 3348
EcoT22I	ATGCAT	2 cut(s)	2323, 2794
ErhI	CCWWGG	7 cut(s)	427, 633, 1750, 2817, 3109, 3165, 3348
FalI	AAGNNNNNCTT	4 cut(s)	1231, 1263, 3374, 3406
FaqI	GGGAC	2 cut(s)	363, 991
FauI	CCCGC	1 cut(s)	2795
FbaI	TGATCA	2 cut(s)	2460, 2563
FblI	GTMKAC	1 cut(s)	3171
GlaI	GCGC	1 cut(s)	2427
GsaI	CCCAGC	1 cut(s)	350
GsuI	CTGGAG	4 cut(s)	278, 2678, 2870, 3056
HaeII	RGCGCY	1 cut(s)	2429
HgaI	GACGC	1 cut(s)	173
HhaI	GCGC	1 cut(s)	2428
Hin1I	GRCGYC	1 cut(s)	158
Hin6I	GCGC	1 cut(s)	2426
HinP1I	GCGC	1 cut(s)	2426
HincII	GTYRAC	5 cut(s)	802, 972, 2157, 2651, 2753
HindII	GTYRAC	5 cut(s)	802, 972, 2157, 2651, 2753
HindIII	AAGCTT	6 cut(s)	76, 794, 1072, 1240, 1298, 2107
HpaI	GTTAAC	2 cut(s)	802, 972
HpyCH4III	ACNGT	5 cut(s)	500, 1220, 1666, 1714, 2638
HpyCH4IV	ACGT	2 cut(s)	158, 3174
HpyF10VI	GCNNNNNNNGC	7 cut(s)	104, 177, 1603, 1657, 2546, 3120, 3382
HpySE526I	ACGT	2 cut(s)	158, 3174
Hsp92I	GRCGYC	1 cut(s)	158
HspAI	GCGC	1 cut(s)	2426
Ksp22I	TGATCA	2 cut(s)	2460, 2563
KspAI	GTTAAC	2 cut(s)	802, 972
LguI	GCTCTTC	1 cut(s)	3366
LmnI	GCTCC	5 cut(s)	362, 703, 1625, 2183, 3418
LweI	GCATC	3 cut(s)	2179, 2330, 3107
MaeII	ACGT	2 cut(s)	158, 3174
MaeIII	GTNAC	3 cut(s)	200, 2048, 3204
MbiI	CCGCTC	1 cut(s)	2802
MboII	GAAGA	7 cut(s)	1220, 1348, 1364, 2669, 2879, 3251, 3383
MfeI	CAATTG	3 cut(s)	181, 1360, 3117
MflI	RGATCY	6 cut(s)	998, 1053, 1193, 1319, 1816, 2344
MlsI	TGGCCA	1 cut(s)	1503
MluNI	TGGCCA	1 cut(s)	1503
MlyI	GAGTC	5 cut(s)	14, 1804, 2720, 2758, 3225
MmeI	TCCRAC	5 cut(s)	1297, 1459, 1926, 1998, 2988
Mox20I	TGGCCA	1 cut(s)	1503
Mph1103I	ATGCAT	2 cut(s)	2323, 2794
MroXI	GAANNNNTTC	6 cut(s)	107, 283, 824, 1435, 1544, 2437
MscI	TGGCCA	1 cut(s)	1503
MslI	CAYNNNNRTG	7 cut(s)	257, 1611, 2133, 2492, 2868, 3033, 3148
Msp20I	TGGCCA	1 cut(s)	1503
MspA1I	CMGCKG	3 cut(s)	341, 931, 1570
MspR9I	CCNGG	7 cut(s)	296, 1422, 1851, 2342, 2696, 2849, 3074
MunI	CAATTG	3 cut(s)	181, 1360, 3117
Mva1269I	GAATGC	4 cut(s)	107, 837, 1923, 2950
MvaI	CCWGG	5 cut(s)	296, 1851, 2696, 2849, 3074
MwoI	GCNNNNNNNGC	7 cut(s)	104, 177, 1603, 1657, 2546, 3120, 3382
NciI	CCSGG	2 cut(s)	1422, 2342
NcoI	CCATGG	1 cut(s)	633
NheI	GCTAGC	1 cut(s)	3385
NlaIV	GGNNCC	6 cut(s)	1195, 1321, 1627, 1771, 1843, 3324
NmuCI	GTSAC	1 cut(s)	200
NsiI	ATGCAT	2 cut(s)	2323, 2794
NspI	RCATGY	5 cut(s)	481, 1882, 1945, 2376, 3151
OliI	CACNNNNGTG	1 cut(s)	257
PaeI	GCATGC	2 cut(s)	481, 1882
PceI	AGGCCT	3 cut(s)	98, 291, 2816
PciI	ACATGT	1 cut(s)	3147
PciSI	GCTCTTC	1 cut(s)	3366
PcsI	WCGNNNNNNNCGW	1 cut(s)	2979
PctI	GAATGC	4 cut(s)	107, 837, 1923, 2950
PdmI	GAANNNNTTC	6 cut(s)	107, 283, 824, 1435, 1544, 2437
PfeI	GAWTC	9 cut(s)	712, 820, 1545, 1996, 2125, 2689, 2878, 2907, 3302
PflMI	CCANNNNNTGG	2 cut(s)	1499, 3274
PfoI	TCCNGGA	2 cut(s)	2694, 3072
PinAI	ACCGGT	1 cut(s)	1694
PleI	GAGTC	5 cut(s)	13, 1804, 2719, 2757, 3225
PpsI	GAGTC	5 cut(s)	13, 1804, 2719, 2757, 3225
PpuMI	RGGWCCY	1 cut(s)	1841
PscI	ACATGT	1 cut(s)	3147
PsiI	TTATAA	2 cut(s)	1152, 1238
Psp5II	RGGWCCY	1 cut(s)	1841
Psp6I	CCWGG	5 cut(s)	294, 1849, 2694, 2847, 3072
PspFI	CCCAGC	1 cut(s)	346
PspGI	CCWGG	5 cut(s)	294, 1849, 2694, 2847, 3072
PspN4I	GGNNCC	6 cut(s)	1195, 1321, 1627, 1771, 1843, 3324
PspPI	GGNCC	8 cut(s)	1390, 1553, 1697, 1841, 2063, 2282, 3025, 3322
PspPPI	RGGWCCY	1 cut(s)	1841
PstI	CTGCAG	3 cut(s)	441, 2021, 2542
PstNI	CAGNNNCTG	4 cut(s)	1282, 1294, 1712, 1856
PsuI	RGATCY	6 cut(s)	998, 1053, 1193, 1319, 1816, 2344
PvuII	CAGCTG	3 cut(s)	341, 931, 1570
RsaI	GTAC	6 cut(s)	887, 1222, 2030, 2635, 2642, 2781
RsaNI	GTAC	6 cut(s)	886, 1221, 2029, 2634, 2641, 2780
RseI	CAYNNNNRTG	7 cut(s)	257, 1611, 2133, 2492, 2868, 3033, 3148
SapI	GCTCTTC	1 cut(s)	3366
Sau96I	GGNCC	8 cut(s)	1390, 1553, 1697, 1841, 2063, 2282, 3025, 3322
ScaI	AGTACT	1 cut(s)	2635
SchI	GAGTC	5 cut(s)	14, 1804, 2720, 2758, 3225
ScrFI	CCNGG	7 cut(s)	296, 1422, 1851, 2342, 2696, 2849, 3074
SfaNI	GCATC	3 cut(s)	2179, 2330, 3107
SfcI	CTRYAG	6 cut(s)	437, 499, 2001, 2017, 2538, 2925
SinI	GGWCC	4 cut(s)	1553, 1697, 1841, 3322
SmiMI	CAYNNNNRTG	7 cut(s)	257, 1611, 2133, 2492, 2868, 3033, 3148
SmlI	CTYRAG	6 cut(s)	581, 1187, 1724, 2366, 2552, 3380
SmoI	CTYRAG	6 cut(s)	581, 1187, 1724, 2366, 2552, 3380
SpeI	ACTAGT	2 cut(s)	1367, 3271
SphI	GCATGC	2 cut(s)	481, 1882
SseBI	AGGCCT	3 cut(s)	98, 291, 2816
SsiI	CCGC	4 cut(s)	239, 524, 2476, 2802
SspI	AATATT	2 cut(s)	2914, 3195
StuI	AGGCCT	3 cut(s)	98, 291, 2816
StyD4I	CCNGG	7 cut(s)	294, 1420, 1849, 2340, 2694, 2847, 3072
StyI	CCWWGG	7 cut(s)	427, 633, 1750, 2817, 3109, 3165, 3348
TaaI	ACNGT	5 cut(s)	500, 1220, 1666, 1714, 2638
TaiI	ACGT	2 cut(s)	161, 3177
TaqI	TCGA	1 cut(s)	93
TatI	WGTACW	4 cut(s)	885, 2028, 2633, 2640
TfiI	GAWTC	9 cut(s)	712, 820, 1545, 1996, 2125, 2689, 2878, 2907, 3302
TscAI	CASTG	5 cut(s)	182, 331, 547, 2751, 2888
TseFI	GTSAC	1 cut(s)	200
Tsp45I	GTSAC	1 cut(s)	200
TspDTI	ATGAA	9 cut(s)	854, 1187, 1461, 1725, 1886, 2880, 2891, 2966, 3056
TspGWI	ACGGA	1 cut(s)	2207
TspRI	CASTG	5 cut(s)	182, 331, 547, 2751, 2888
Van91I	CCANNNNNTGG	2 cut(s)	1499, 3274
VpaK11BI	GGWCC	4 cut(s)	1553, 1697, 1841, 3322
XagI	CCTNNNNNAGG	1 cut(s)	1187
XbaI	TCTAGA	2 cut(s)	1381, 1669
XceI	RCATGY	5 cut(s)	481, 1882, 1945, 2376, 3151
XcmI	CCANNNNNNNNNTGG	2 cut(s)	640, 3116
XmiI	GTMKAC	1 cut(s)	3171
XmnI	GAANNNNTTC	6 cut(s)	107, 283, 824, 1435, 1544, 2437
ZraI	GACGTC	1 cut(s)	159
ZrmI	AGTACT	1 cut(s)	2635
Zsp2I	ATGCAT	2 cut(s)	2323, 2794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.