Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008065

Physical Location & Seq

Genomic Coordinates Forward (+)

42164570 .. 42168729

Gene Structure 4160 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1289000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3474 bp

ATGGTGTCTCAGAGATTAAGTTTGGTCGTAGTCTTACTCTGTTCTGCTCTACTTACTGTTCATTCGGCTCAGCCAAGCTTGGAGGTTGAAGTTGAGGCCTTGCATGCATTCAAGAAATCGATCCACGGCGACCCCAACAGAGCACTTGCAGACTGGACAGATGCAAACCATCACTGCAATTGGTCTGGTGTTGCCTGTGGCCCTTCCACAAATCGTGTCATTTCGATTTCCATGTATGACAAGCAGCTCAGCGGTCAGATATCACCGTTCCTCGGAAACATCTCAGGCCTCCAGGTTTTTGATTTAACTTTAAATTCATTCACTGGACATATTCCAGCTGAGCTGGGGCTTTGCTCCCAACTCTCTGAACTTATTTTGTATCAAAATGCTCTTTCGGGTCCAATTCCTCCAGAGCTAGGCAACCTTGGAAATCTTCGGGCATTAGATTTAGGTGATAACTCTTTAACTGGAAGCATCCCGGAGAGCATTTGCAACTGCAAAAACCTGTCACAGTTTGGTGCCAATTCTAATAATCTCACCGGAAAAATCCCATCAAATATAGGAAATCTTGTTAATCTTCAGCTCTTCGCGGTGTATCGCAACAACTTGGTAGGCTCAATACCTGCTTCCATTGGTAGGTTGAGAGTTTTGGAAGCTCTAGATTTAAGTCAAAACCAGTTGTCAGGTGTAATGCCCAGAGAGCTAGGGAATTTGTCAAATTTAGAAATGCTTCTCTTGCTTGAGAATTCCTTTTTCGGGAATATCCCTAGCGAACTAGGTCGTTGTAAGAAGCTTGTTAATCTTGAACTCTATGACAATCAGTTCACCGGAGGCATTCCTTCTGAACTTGGAGATTTGCTTCACTTAGAAACATTGAGATTGTACAACAATGGTCTAAATTCCACAATCCCACTTTCCATTTTTCAGTTGACGTCGTTAACCCATTTAGGACTTTCACAGAATGAGTTAACCGGAACAATACCAGCCGAGATTGAATCTTTAAGATCATTGCAAGTACTGACCCTGCATTCCAACAAGTTCACCGGCGAGATCCCTTCATCCTTGACAAGCCTGACAAATCTAACATATTTGTCAATGGGCATCAATTTCCTAACCGGGGAGCTTCCATCAAATATCGGGTTGCTTTATAATCTGAAAAACCTGACCATGAACAGCAACTTTCTTGAGGGATCCATTCCATCAAGTATCACCAATTGTACACATCTTTTAGTTATAAGCTTAGCTTATAACAGAATAACAGGGAAAATTCCACAGGGGCTGGGGCAGTTACCAAATCTTACTTTCCTTTCGTTTGGATCAAATAAGTTGTATGGAGAAATCCCAGATGACCTCTTCAATTGTACAAGCCTAAAAACTCTAGATTTGGCCATGAACAACTTTAGCGGTTTGCTGAAACCAGGAATTAGAAAACTCTCAAATCTCAGGCTTTTTAATGCCATCTCAAATTCATTTGTCGGGCCAATTCCACCAGAAATTGGCCAATTAAGCCAACTTATCCTCCTTTCACTTGCTGAGAATAGCTTTTCGGGTCCGGTTCCACCACAGTTATCTAAGCTCTCTCAGCTCCAAGGGCTTTCCCTGCACGACAATGCTTTAGAAGGAGCCATACCTGAGGAAATTTTTAAACTGAAACAGCTAACCAATCTTCAGTTGCAACACAATAAACTGACGGGTCCAATTCCAGATTCTGTGTCAAAACTTGAGTTGCTTTCATACTTAAACCTCGGGCATAATATGCTCAATGGTTCCATTCCAAAAAGCATGGCTGGCCTTAATCGGCTGACAACCCTGGATCTCTCTCACAACCGTCTCTCGGGATCCATTCCTGGATCAGTGGTCTCAAGCATGAGAAGCATGCAGATATATCTGAACTTCTCATACAATTTCTTGGATGGCACCATTCCGGATGAGCTTGGTAAGTTGGAAATGGTACAAGCCATTGACATTTCCAACAATAATCTCTCTGGAATCATTCCCAGAACAATAGAAGGCTGCAAGAACTTGTTCTCTTTTGATCTATCGGGGAACAAACTTTCTGGTTCAATTCCAGTTGAACCTTTTGCCCAGATGGACATCCTCACAAGCTTGAATCTTTCAAGGAACAACTTAGATGGTGAAATCCCGGAAAAGTTATCAAATCTGAAGCATCTTAGTTCCCTTGACCTTTCTCAAAATCATCTGAGAGGCATTATCCCTGAGAACTTTGCCAATCTTTCCAGCTTGAAGCACTTAAATGTCTCTTTCAATGGACTTCAAGGCCATGTTCCAGACAGTGGAATATTCAAAAAGATGAATGTGTCCAGCTTGATGGGGAACCCAGATCTCTGTGGTAACAAATTTCTCAAGATATGCAAAAAAAGTTCACACCGGCTGTCCAAGAAAACCAAGTTTATCCTGTTGGTGCTTGGATCTGCTTCTCTACTTCTGGTTCTAGTGCTCAGTATTCACATTCTCCACCGCTTCACCAACTCACATAGACCAGAAAAGCTTGAGGATCCAGAATATGAGTATGCTTCAGCATTGACCCTCAAGAGGTTTGATCAAAAGGATTTAGAAACTGCCACCGGTTGCTTCAGCAAAGATAATATTATAGGTGCAAGCAATTTGAGCACTGTGTACAAGGGTGAACTAGAAGACGGGCAGATGGTAGCCATAAAGAGATTGAATCTGCACCAGTTCTCTGTCGAGTCTGATAAATGCTTCAATAGAGAAATCAAGACACTGTCCCAACTACGGCATAGGAATTTGGTGAAGGTACTCGGTTATGCATGGGAGAGCATGAAGTTAAAGGCCTTGGTCCTGACGTACATGAAGAACGGGAACCTGGACAGCATTATACATGAAGATGAGTTGAATAATTGGAAATGCACATTATCTGAGAGAACCAACATTTTAATTTCAATAGCAAGCGGATTGGATTACCTGCATTCGGGTTATGATTTTCCGATTGTTCATTGTGACTTGAAGCCTTCTAACATATTGTTGGATGATGACTGGGTGGCCCATGTGAGTGATTTCGGGACAGCTAGAATGTTGGGTGTTCATCTCCAGGACGGGAGTAGCCTTTCCTCAGCATCAGCTTTCCAAGGAACAATTGGCTACCTGGCACCAGAATTTGCATATATGAGGAAAATCACAACTAAGGTCGACTTATTCAGCTTTGGTATAATAGTGATGGAGCTGCTGACAAAGAAAAGGCCAACAGGACTTATGGAAGAGAATGGGCTGCCAGTGAGCTTGTCTCAACTTGTGGAGAAATCCGTTGCAAATGGAACGAAGACGCTTCTTCAAGTTCTGGACCCTATGCTAGCTTCAAATATCTCAACGGAAGGGGAGAAAATTGCAGAAGAGCTACTTAAGATAGCCTTGCTTTGCACCAACCCGGATCCTGACAACCGACCTAACATGAATGAGGTGTTGACCACCCTTCTGAAGCTGAAAAGGGAAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006898 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010359 GO:0012505 GO:0016020 GO:0016043 GO:0016045 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031410 GO:0031982 GO:0032409 GO:0032412 GO:0032879 GO:0033036 GO:0033037 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0042545 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065009 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098542 GO:0098543 GO:0098581 GO:0098657 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1158

Amino Acids

126.73

Weight (kDa)

6.26

Isoelectric Point (pI)

31.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 66	1.5e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	181 - 270	1.3e-08	Leucine-rich repeat region
LRR_14	PF23598	260 - 367	1.2e-10	Leucine-rich repeat region
LRR_14	PF23598	316 - 391	3e-07	Leucine-rich repeat region
LRR_8	PF13855	384 - 443	1.4e-06	Leucine rich repeat
LRR_14	PF23598	498 - 627	1.4e-08	Leucine-rich repeat region
LRR_8	PF13855	552 - 611	1e-10	Leucine rich repeat
LRR_8	PF13855	633 - 685	2.6e-06	Leucine rich repeat
LRR_8	PF13855	688 - 734	2.4e-06	Leucine rich repeat
LRR_8	PF13855	700 - 758	4.2e-09	Leucine rich repeat
Pkinase	PF00069	866 - 1148	2.6e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	868 - 1150	3.6e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46330 AT5G46330
fragaria_vesca	FvH4_3g06100
malus_domestica	MD05G1289000.v1.1
prunus_persica	Prupe.4G076500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0013311
rosa_laevigata	RLG00000032007 RLG00000032011
rosa_multiflora	Rmu_co8051088.1_g000001 Rmu_sc0003458.1_g000007 Rmu_sc0003458.1_g000011 Rmu_sc0004521.1_g000021 Rmu_sc0007948.1_g000008
rosa_roxburghii	Rroxscaffold_1G00063230
rosa_rugosa	Rorug05G0008800
rosa_samantha	Rh5AG103400 Rh5AG103500 Rh5AG104200 Rh5BG100200 Rh5CG111400 Rh5CG111800 Rh5DG099000
rosa_wichuraiana	Rw5G009030 Rw5G009070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1149, 1235, 1248
AatII	GACGTC	1 cut(s)	935
Acc36I	ACCTGC	2 cut(s)	631, 2952
AccB1I	GGYRCC	3 cut(s)	518, 1916, 3127
AccB7I	CCANNNNNTGG	2 cut(s)	1496, 2294
AccI	GTMKAC	1 cut(s)	3168
AccII	CGCG	1 cut(s)	590
AccIII	TCCGGA	1 cut(s)	1924
AciI	CCGC	5 cut(s)	252, 590, 1404, 2479, 2931
AcoI	YGGCCR	2 cut(s)	1386, 1498
AcuI	CTGAAG	6 cut(s)	563, 1652, 2183, 2520, 2578, 3473
AcyI	GRCGYC	1 cut(s)	932
AfaI	GTAC	8 cut(s)	884, 1017, 1219, 1363, 1953, 2639, 2778, 2828
AflII	CTTAAG	1 cut(s)	3377
AgeI	ACCGGT	1 cut(s)	2585
AjnI	CCWGG	7 cut(s)	291, 1417, 1809, 1846, 2844, 3069, 3123
AjuI	GAANNNNNNNTTGG	2 cut(s)	1690, 1722
Alw21I	GWGCWC	3 cut(s)	145, 2460, 2633
Alw26I	GTCTC	5 cut(s)	12, 1835, 1864, 2263, 3267
AlwNI	CAGNNNCTG	2 cut(s)	1279, 1709
Ama87I	CYCGRG	2 cut(s)	1745, 1834
Aor13HI	TCCGGA	1 cut(s)	1924
ApeKI	GCWGC	4 cut(s)	244, 2013, 3202, 3247
AsiGI	ACCGGT	1 cut(s)	2585
Asp700I	GAANNNNTTC	2 cut(s)	729, 2024
AspS9I	GGNCC	8 cut(s)	200, 398, 1478, 1550, 1694, 2818, 3022, 3319
AsuC2I	CCSGG	4 cut(s)	479, 1117, 2144, 3404
AsuNHI	GCTAGC	1 cut(s)	3328
AvaI	CYCGRG	2 cut(s)	1745, 1834
AvaII	GGWCC	5 cut(s)	398, 1550, 1694, 2818, 3319
AxyI	CCTNAGG	1 cut(s)	1632
BalI	TGGCCA	2 cut(s)	1388, 1500
BamHI	GGATCC	4 cut(s)	1190, 1838, 2515, 3406
BanI	GGYRCC	3 cut(s)	518, 1916, 3127
BarI	GAAGNNNNNNTAC	2 cut(s)	1611, 1643
BbsI	GAAGAC	2 cut(s)	2661, 3305
Bbv12I	GWGCWC	3 cut(s)	145, 2460, 2633
BbvCI	CCTCAGC	1 cut(s)	3091
BbvI	GCAGC	4 cut(s)	256, 2000, 3189, 3234
BceAI	ACGGC	2 cut(s)	142, 2771
BciT130I	CCWGG	7 cut(s)	293, 1419, 1811, 1848, 2846, 3071, 3125
BclI	TGATCA	1 cut(s)	2560
BcnI	CCSGG	4 cut(s)	479, 1117, 2144, 3404
BcoDI	GTCTC	5 cut(s)	12, 1835, 1864, 2263, 3267
BfrI	CTTAAG	1 cut(s)	3377
BfuAI	ACCTGC	2 cut(s)	631, 2952
BglII	AGATCT	1 cut(s)	2341
BisI	GCNGC	4 cut(s)	245, 2014, 3203, 3248
BlpI	GCTNAGC	4 cut(s)	69, 248, 339, 1240
BlsI	GCNGC	4 cut(s)	246, 2015, 3204, 3249
BmcAI	AGTACT	1 cut(s)	1017
Bme18I	GGWCC	5 cut(s)	398, 1550, 1694, 2818, 3319
BmeT110I	CYCGRG	2 cut(s)	1745, 1834
BmgT120I	GGNCC	8 cut(s)	200, 398, 1478, 1550, 1694, 2818, 3022, 3319
BmrI	ACTGGG	1 cut(s)	3025
BmsI	GCATC	5 cut(s)	151, 483, 1110, 2176, 3104
BmtI	GCTAGC	1 cut(s)	3332
BmuI	ACTGGG	1 cut(s)	3025
BpiI	GAAGAC	2 cut(s)	2661, 3305
BplI	GAGNNNNNCTC	2 cut(s)	3247, 3279
BpmI	CTGGAG	3 cut(s)	275, 393, 3053
Bpu10I	CCTNAGC	1 cut(s)	3091
Bpu1102I	GCTNAGC	4 cut(s)	69, 248, 339, 1240
BpuEI	CTTGAG	7 cut(s)	761, 1205, 1742, 1846, 2348, 2531, 2534
BpuMI	CCSGG	4 cut(s)	479, 1117, 2144, 3404
Bsa29I	ATCGAT	1 cut(s)	119
BsaBI	GATNNNNATC	1 cut(s)	2093
BsaHI	GRCGYC	1 cut(s)	932
BsaI	GGTCTC	1 cut(s)	1864
BsaJI	CCNNGG	9 cut(s)	124, 271, 424, 1116, 1588, 1744, 1809, 2814, 3106
BsaWI	WCCGGW	6 cut(s)	539, 827, 971, 1552, 1924, 2585
BsaXI	ACNNNNNCTCC	2 cut(s)	1503, 1533
Bse118I	RCCGGY	3 cut(s)	1043, 2388, 2585
Bse1I	ACTGG	8 cut(s)	158, 328, 472, 676, 2069, 2695, 3020, 3251
Bse21I	CCTNAGG	1 cut(s)	1632
Bse3DI	GCAATG	1 cut(s)	1007
Bse8I	GATNNNNATC	1 cut(s)	2093
BseAI	TCCGGA	1 cut(s)	1924
BseBI	CCWGG	7 cut(s)	293, 1419, 1811, 1848, 2846, 3071, 3125
BseCI	ATCGAT	1 cut(s)	119
BseDI	CCNNGG	9 cut(s)	124, 271, 424, 1116, 1588, 1744, 1809, 2814, 3106
BseGI	GGATG	6 cut(s)	474, 1058, 1918, 1933, 2094, 3013
BseJI	GATNNNNATC	1 cut(s)	2093
BseMI	GCAATG	1 cut(s)	1007
BseNI	ACTGG	8 cut(s)	158, 328, 472, 676, 2069, 2695, 3020, 3251
BseXI	GCAGC	4 cut(s)	256, 2000, 3189, 3234
BseYI	CCCAGC	2 cut(s)	343, 1279
BsgI	GTGCAG	2 cut(s)	1586, 2675
Bsh1236I	CGCG	1 cut(s)	590
BshNI	GGYRCC	3 cut(s)	518, 1916, 3127
BshTI	ACCGGT	1 cut(s)	2585
BshVI	ATCGAT	1 cut(s)	119
BsiHKAI	GWGCWC	3 cut(s)	145, 2460, 2633
BsiHKCI	CYCGRG	2 cut(s)	1745, 1834
BslFI	GGGAC	2 cut(s)	2731, 3055
BsmAI	GTCTC	5 cut(s)	12, 1835, 1864, 2263, 3267
BsmBI	CGTCTC	1 cut(s)	1835
BsmFI	GGGAC	2 cut(s)	2731, 3055
BsmI	GAATGC	4 cut(s)	107, 834, 1027, 2947
Bso31I	GGTCTC	1 cut(s)	1864
BsoBI	CYCGRG	2 cut(s)	1745, 1834
Bsp1286I	GDGCHC	3 cut(s)	145, 2460, 2633
Bsp13I	TCCGGA	1 cut(s)	1924
Bsp1407I	TGTACA	4 cut(s)	882, 1217, 1361, 2637
Bsp1720I	GCTNAGC	4 cut(s)	69, 248, 339, 1240
BspACI	CCGC	5 cut(s)	252, 590, 1404, 2479, 2931
BspDI	ATCGAT	1 cut(s)	119
BspEI	TCCGGA	1 cut(s)	1924
BspFNI	CGCG	1 cut(s)	590
BspMI	ACCTGC	2 cut(s)	631, 2952
BspOI	GCTAGC	1 cut(s)	3332
BspQI	GCTCTTC	2 cut(s)	590, 3363
BspT107I	GGYRCC	3 cut(s)	518, 1916, 3127
BspTI	CTTAAG	1 cut(s)	3377
BspTNI	GGTCTC	1 cut(s)	1864
BsrDI	GCAATG	1 cut(s)	1007
BsrFI	RCCGGY	3 cut(s)	1043, 2388, 2585
BsrGI	TGTACA	4 cut(s)	882, 1217, 1361, 2637
BsrI	ACTGG	8 cut(s)	158, 328, 472, 676, 2069, 2695, 3020, 3251
BssAI	RCCGGY	3 cut(s)	1043, 2388, 2585
BssECI	CCNNGG	9 cut(s)	124, 271, 424, 1116, 1588, 1744, 1809, 2814, 3106
BssNI	GRCGYC	1 cut(s)	932
BssT1I	CCWWGG	4 cut(s)	424, 1588, 2814, 3106
Bst2UI	CCWGG	7 cut(s)	293, 1419, 1811, 1848, 2846, 3071, 3125
Bst4CI	ACNGT	8 cut(s)	58, 267, 513, 1566, 1829, 2294, 2635, 2745
Bst6I	CTCTTC	4 cut(s)	590, 1358, 3231, 3363
BstACI	GRCGYC	1 cut(s)	932
BstAFI	CTTAAG	1 cut(s)	3377
BstAPI	GCANNNNNTGC	1 cut(s)	1756
BstAUI	TGTACA	4 cut(s)	882, 1217, 1361, 2637
BstC8I	GCNNGC	6 cut(s)	105, 1789, 1877, 2620, 2929, 3330
BstDSI	CCRYGG	1 cut(s)	124
BstF5I	GGATG	6 cut(s)	474, 1058, 1918, 1933, 2094, 3013
BstFNI	CGCG	1 cut(s)	590
BstMAI	GTCTC	5 cut(s)	12, 1835, 1864, 2263, 3267
BstNI	CCWGG	7 cut(s)	293, 1419, 1811, 1848, 2846, 3071, 3125
BstNSI	RCATGY	2 cut(s)	107, 1879
BstUI	CGCG	1 cut(s)	590
BstV1I	GCAGC	4 cut(s)	256, 2000, 3189, 3234
BstV2I	GAAGAC	2 cut(s)	2661, 3305
BstX2I	RGATCY	8 cut(s)	1050, 1190, 1813, 1838, 2341, 2429, 2515, 3406
BstXI	CCANNNNNNTGG	1 cut(s)	2329
BstYI	RGATCY	8 cut(s)	1050, 1190, 1813, 1838, 2341, 2429, 2515, 3406
Bsu15I	ATCGAT	1 cut(s)	119
Bsu36I	CCTNAGG	1 cut(s)	1632
BsuTUI	ATCGAT	1 cut(s)	119
BtgI	CCRYGG	1 cut(s)	124
BtsCI	GGATG	6 cut(s)	474, 1058, 1918, 1933, 2094, 3013
BtsI	GCAGTG	1 cut(s)	172
BtsIMutI	CAGTG	7 cut(s)	172, 321, 1860, 2299, 2631, 2741, 3258
BveI	ACCTGC	2 cut(s)	631, 2952
Cac8I	GCNNGC	6 cut(s)	105, 1789, 1877, 2620, 2929, 3330
CaiI	CAGNNNCTG	2 cut(s)	1279, 1709
Cfr10I	RCCGGY	3 cut(s)	1043, 2388, 2585
Cfr13I	GGNCC	8 cut(s)	200, 398, 1478, 1550, 1694, 2818, 3022, 3319
ClaI	ATCGAT	1 cut(s)	119
CseI	GACGC	1 cut(s)	3310
Csp6I	GTAC	8 cut(s)	883, 1016, 1218, 1362, 1952, 2638, 2777, 2827
CspAI	ACCGGT	1 cut(s)	2585
CviQI	GTAC	8 cut(s)	883, 1016, 1218, 1362, 1952, 2638, 2777, 2827
DraI	TTTAAA	2 cut(s)	312, 1645
EaeI	YGGCCR	2 cut(s)	1386, 1498
Eam1104I	CTCTTC	4 cut(s)	590, 1358, 3231, 3363
EarI	CTCTTC	4 cut(s)	590, 1358, 3231, 3363
Eco130I	CCWWGG	4 cut(s)	424, 1588, 2814, 3106
Eco147I	AGGCCT	3 cut(s)	98, 288, 2813
Eco31I	GGTCTC	1 cut(s)	1864
Eco32I	GATATC	1 cut(s)	261
Eco47I	GGWCC	5 cut(s)	398, 1550, 1694, 2818, 3319
Eco57I	CTGAAG	6 cut(s)	563, 1652, 2183, 2520, 2578, 3473
Eco81I	CCTNAGG	1 cut(s)	1632
Eco88I	CYCGRG	2 cut(s)	1745, 1834
EcoRI	GAATTC	1 cut(s)	745
EcoRII	CCWGG	7 cut(s)	291, 1417, 1809, 1846, 2844, 3069, 3123
EcoRV	GATATC	1 cut(s)	261
EcoT14I	CCWWGG	4 cut(s)	424, 1588, 2814, 3106
EcoT22I	ATGCAT	2 cut(s)	109, 2791
ErhI	CCWWGG	4 cut(s)	424, 1588, 2814, 3106
Esp3I	CGTCTC	1 cut(s)	1835
FalI	AAGNNNNNCTT	8 cut(s)	1228, 1260, 3155, 3187, 3360, 3392, 3371, 3403
FaqI	GGGAC	2 cut(s)	2731, 3055
FbaI	TGATCA	1 cut(s)	2560
FblI	GTMKAC	1 cut(s)	3168
Fnu4HI	GCNGC	4 cut(s)	245, 2014, 3203, 3248
FokI	GGATG	6 cut(s)	461, 1045, 1925, 1940, 2081, 3020
Fsp4HI	GCNGC	4 cut(s)	245, 2014, 3203, 3248
GluI	GCNGC	4 cut(s)	245, 2014, 3203, 3248
GsaI	CCCAGC	2 cut(s)	347, 1283
GsuI	CTGGAG	3 cut(s)	275, 393, 3053
HgaI	GACGC	1 cut(s)	3310
Hin1I	GRCGYC	1 cut(s)	932
HincII	GTYRAC	5 cut(s)	930, 939, 969, 3169, 3441
HindII	GTYRAC	5 cut(s)	930, 939, 969, 3169, 3441
HindIII	AAGCTT	5 cut(s)	76, 791, 1237, 2104, 2507
HinfI	GANTC	6 cut(s)	995, 1706, 1989, 2110, 2686, 2708
HpaI	GTTAAC	2 cut(s)	939, 969
Hpy99I	CGWCG	1 cut(s)	937
HpyAV	CCTTC	9 cut(s)	213, 849, 1065, 1613, 2003, 2767, 3000, 3344, 3458
HpyCH4III	ACNGT	8 cut(s)	58, 267, 513, 1566, 1829, 2294, 2635, 2745
HpyCH4IV	ACGT	2 cut(s)	932, 2825
HpySE526I	ACGT	2 cut(s)	932, 2825
Hsp92I	GRCGYC	1 cut(s)	932
Kpn2I	TCCGGA	1 cut(s)	1924
Ksp22I	TGATCA	1 cut(s)	2560
KspAI	GTTAAC	2 cut(s)	939, 969
LguI	GCTCTTC	2 cut(s)	590, 3363
LmnI	GCTCC	5 cut(s)	359, 1120, 1590, 1622, 3199
Lsp1109I	GCAGC	4 cut(s)	256, 2000, 3189, 3234
LweI	GCATC	5 cut(s)	151, 483, 1110, 2176, 3104
MaeII	ACGT	2 cut(s)	932, 2825
MaeIII	GTNAC	4 cut(s)	507, 1287, 2351, 2978
MfeI	CAATTG	4 cut(s)	178, 1213, 1357, 3114
MflI	RGATCY	8 cut(s)	1050, 1190, 1813, 1838, 2341, 2429, 2515, 3406
MhlI	GDGCHC	3 cut(s)	145, 2460, 2633
MlsI	TGGCCA	2 cut(s)	1388, 1500
MluNI	TGGCCA	2 cut(s)	1388, 1500
MlyI	GAGTC	1 cut(s)	2717
MmeI	TCCRAC	4 cut(s)	1056, 1923, 1995, 2985
Mox20I	TGGCCA	2 cut(s)	1388, 1500
Mph1103I	ATGCAT	2 cut(s)	109, 2791
MroI	TCCGGA	1 cut(s)	1924
MroXI	GAANNNNTTC	2 cut(s)	729, 2024
MscI	TGGCCA	2 cut(s)	1388, 1500
MslI	CAYNNNNRTG	4 cut(s)	1608, 2253, 2865, 3030
Msp20I	TGGCCA	2 cut(s)	1388, 1500
MspA1I	CMGCKG	2 cut(s)	252, 338
MspCI	CTTAAG	1 cut(s)	3377
MunI	CAATTG	4 cut(s)	178, 1213, 1357, 3114
Mva1269I	GAATGC	4 cut(s)	107, 834, 1027, 2947
MvaI	CCWGG	7 cut(s)	293, 1419, 1811, 1848, 2846, 3071, 3125
MvnI	CGCG	1 cut(s)	590
NciI	CCSGG	4 cut(s)	479, 1117, 2144, 3404
NheI	GCTAGC	1 cut(s)	3328
NmeAIII	GCCGAG	1 cut(s)	1012
NmuCI	GTSAC	2 cut(s)	507, 2978
NsiI	ATGCAT	2 cut(s)	109, 2791
NspI	RCATGY	2 cut(s)	107, 1879
PaeI	GCATGC	2 cut(s)	107, 1879
PceI	AGGCCT	3 cut(s)	98, 288, 2813
PciSI	GCTCTTC	2 cut(s)	590, 3363
PctI	GAATGC	4 cut(s)	107, 834, 1027, 2947
PdmI	GAANNNNTTC	2 cut(s)	729, 2024
PfeI	GAWTC	5 cut(s)	995, 1706, 1989, 2110, 2686
PflFI	GACNNNGTC	1 cut(s)	2743
PflMI	CCANNNNNTGG	2 cut(s)	1496, 2294
PfoI	TCCNGGA	4 cut(s)	477, 1846, 2142, 3069
PinAI	ACCGGT	1 cut(s)	2585
PkrI	GCNGC	4 cut(s)	246, 2015, 3204, 3249
PleI	GAGTC	1 cut(s)	2716
PpsI	GAGTC	1 cut(s)	2716
PsiI	TTATAA	3 cut(s)	1149, 1235, 1248
Psp6I	CCWGG	7 cut(s)	291, 1417, 1809, 1846, 2844, 3069, 3123
PspFI	CCCAGC	2 cut(s)	343, 1279
PspGI	CCWGG	7 cut(s)	291, 1417, 1809, 1846, 2844, 3069, 3123
PspPI	GGNCC	8 cut(s)	200, 398, 1478, 1550, 1694, 2818, 3022, 3319
PsrI	GAACNNNNNNTAC	2 cut(s)	1883, 1915
PstNI	CAGNNNCTG	2 cut(s)	1279, 1709
PsuI	RGATCY	8 cut(s)	1050, 1190, 1813, 1838, 2341, 2429, 2515, 3406
PsyI	GACNNNGTC	1 cut(s)	2743
PvuII	CAGCTG	1 cut(s)	338
RsaI	GTAC	8 cut(s)	884, 1017, 1219, 1363, 1953, 2639, 2778, 2828
RsaNI	GTAC	8 cut(s)	883, 1016, 1218, 1362, 1952, 2638, 2777, 2827
RseI	CAYNNNNRTG	4 cut(s)	1608, 2253, 2865, 3030
SalI	GTCGAC	1 cut(s)	3167
SapI	GCTCTTC	2 cut(s)	590, 3363
SatI	GCNGC	4 cut(s)	245, 2014, 3203, 3248
Sau96I	GGNCC	8 cut(s)	200, 398, 1478, 1550, 1694, 2818, 3022, 3319
ScaI	AGTACT	1 cut(s)	1017
SchI	GAGTC	1 cut(s)	2717
SduI	GDGCHC	3 cut(s)	145, 2460, 2633
SfaNI	GCATC	5 cut(s)	151, 483, 1110, 2176, 3104
SgrAI	CRCCGGYG	1 cut(s)	1043
SinI	GGWCC	5 cut(s)	398, 1550, 1694, 2818, 3319
SmiMI	CAYNNNNRTG	4 cut(s)	1608, 2253, 2865, 3030
SmlI	CTYRAG	8 cut(s)	740, 1184, 1721, 1861, 2363, 2510, 2549, 3377
SmoI	CTYRAG	8 cut(s)	740, 1184, 1721, 1861, 2363, 2510, 2549, 3377
SphI	GCATGC	2 cut(s)	107, 1879
SseBI	AGGCCT	3 cut(s)	98, 288, 2813
SsiI	CCGC	5 cut(s)	252, 590, 1404, 2479, 2931
SspI	AATATT	2 cut(s)	2301, 2608
StuI	AGGCCT	3 cut(s)	98, 288, 2813
StyI	CCWWGG	4 cut(s)	424, 1588, 2814, 3106
TaaI	ACNGT	8 cut(s)	58, 267, 513, 1566, 1829, 2294, 2635, 2745
TaiI	ACGT	2 cut(s)	935, 2828
TaqI	TCGA	4 cut(s)	119, 224, 2706, 3168
TatI	WGTACW	5 cut(s)	882, 1015, 1217, 1361, 2637
TfiI	GAWTC	5 cut(s)	995, 1706, 1989, 2110, 2686
TscAI	CASTG	7 cut(s)	179, 328, 1860, 2299, 2638, 2748, 3258
TseFI	GTSAC	2 cut(s)	507, 2978
TseI	GCWGC	4 cut(s)	244, 2013, 3202, 3247
Tsp45I	GTSAC	2 cut(s)	507, 2978
TspGWI	ACGGA	2 cut(s)	3271, 3362
TspRI	CASTG	7 cut(s)	179, 328, 1860, 2299, 2638, 2748, 3258
Tth111I	GACNNNGTC	1 cut(s)	2743
Van91I	CCANNNNNTGG	2 cut(s)	1496, 2294
Vha464I	CTTAAG	1 cut(s)	3377
VpaK11BI	GGWCC	5 cut(s)	398, 1550, 1694, 2818, 3319
XbaI	TCTAGA	2 cut(s)	658, 1378
XceI	RCATGY	2 cut(s)	107, 1879
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3113
XmiI	GTMKAC	1 cut(s)	3168
XmnI	GAANNNNTTC	2 cut(s)	729, 2024
ZraI	GACGTC	1 cut(s)	933
ZrmI	AGTACT	1 cut(s)	1017
Zsp2I	ATGCAT	2 cut(s)	109, 2791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.