FvH4_3g07360
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011269
Physical Location & Seq
Reverse (-)
4360124 .. 4362319
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07360.t2

Sequence Viewer

Length: 834 bp
ATGGGCTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGACTACAACACCGCCACCACCTCCACCCCCGAAAACAGCTCCGGCTCCCCTCCTCCTCCTCCCGCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCCTCCCCCAAGAAAAGCAGCCGGCGAGCCATACAGAAGAGAGTCGTGTCCATCCCCATCAAGGAAAACAGTAACACGAACACTCCGCCGTCTGATTCCTGGGCCTGGCGGAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCTAGGGGATATTACAGGTGCAGTAGCTCAAAAGGGTGCCCAGCGAGGAAGCAGGTGGAGAGAAGCCGAGCGGACCCCACTATGCTGATGGTCACCTACTCTTCGGAACACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCGCAAAACCCCAGACCAAAACCGAGGTCCGGGAGGAGGAGGAGGAGGAGACAGCGGCGGCCCAGCCGGAGCCCGACCCGGAGGAGAGGCGGTTCGCTGATCTGGGCGGCGACGAGGAGCCGGCCCTGGTGATCATCCCGAGCGCCGCCGACGAGTTCGGGTGGTTCGCCGCCGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCGATTTTCACGGAGAGCGGTGGGCCCGACTTTGGTGACGTGGCGGGGATGGTGTTCCCGATGGGCGAGGAGGACGAGTCGTTATTCGCCGATCTCGGGGAGCTGCCCGAGTGCTCGTTTGTGTTCCGGCACCGCCGTGTCGGAGGCGGAGGAGGTGTGGGACCACAAGTTCAGATCTGTTGA

Protein Analysis

278

Amino Acids

30.43

Weight (kDa)

5.19

Isoelectric Point (pI)

65.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 81 - 138 1.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
AatII GACGTC 2 cut(s) 132, 632
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 333, 780
AccBSI CCGCTC 2 cut(s) 368, 669
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 637
AcoI YGGCCR 2 cut(s) 268, 419
AcyI GRCGYC 2 cut(s) 129, 629
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 8 cut(s) 211, 255, 284, 421, 471, 478, 683, 747
AgsI TTSAA 2 cut(s) 22, 124
AjiI CACGTC 1 cut(s) 691
AjnI CCWGG 4 cut(s) 248, 254, 416, 567
AleI CACNNNNGTG 1 cut(s) 786
AloI GAACNNNNNNTCC 3 cut(s) 518, 550, 804
AluBI AGCT 3 cut(s) 93, 324, 754
AluI AGCT 3 cut(s) 93, 324, 754
Alw21I GWGCWC 1 cut(s) 767
Alw26I GTCTC 1 cut(s) 485
Ama87I CYCGRG 3 cut(s) 580, 746, 758
AoxI GGCC 6 cut(s) 252, 268, 419, 501, 564, 674
ApaI GGGCCC 1 cut(s) 678
ApeKI GCWGC 2 cut(s) 168, 754
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AspA2I CCTAGG 1 cut(s) 299
AspLEI GCGC 2 cut(s) 446, 587
AspS9I GGNCC 8 cut(s) 252, 370, 469, 502, 565, 674, 675, 812
AsuC2I CCSGG 2 cut(s) 473, 521
AsuHPI GGTGA 4 cut(s) 282, 382, 583, 698
AvaI CYCGRG 3 cut(s) 580, 746, 758
AvaII GGWCC 3 cut(s) 370, 469, 812
AvrII CCTAGG 1 cut(s) 299
BaeGI GKGCMC 2 cut(s) 338, 678
BanI GGYRCC 2 cut(s) 333, 780
BanII GRGCYC 5 cut(s) 8, 290, 516, 653, 678
Bbv12I GWGCWC 1 cut(s) 767
BbvI GCAGC 2 cut(s) 180, 741
BccI CCATC 6 cut(s) 209, 215, 287, 379, 694, 706
BceAI ACGGC 4 cut(s) 223, 283, 457, 771
BciT130I CCWGG 4 cut(s) 250, 256, 418, 569
BclI TGATCA 2 cut(s) 46, 573
BcnI CCSGG 2 cut(s) 473, 521
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 2 cut(s) 300, 429
BfoI RGCGCY 1 cut(s) 588
BfuAI ACCTGC 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 825
BisI GCNGC 7 cut(s) 169, 498, 501, 550, 588, 612, 755
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 7 cut(s) 170, 499, 502, 551, 589, 613, 756
Bme1390I CCNGG 6 cut(s) 250, 256, 418, 473, 521, 569
Bme18I GGWCC 3 cut(s) 370, 469, 812
BmeT110I CYCGRG 3 cut(s) 580, 746, 758
BmgBI CACGTC 1 cut(s) 691
BmgT120I GGNCC 8 cut(s) 252, 370, 469, 502, 565, 674, 675, 812
BmiI GGNNCC 9 cut(s) 7, 100, 335, 372, 513, 561, 676, 782, 813
BmrFI CCNGG 6 cut(s) 250, 256, 418, 473, 521, 569
BpmI CTGGAG 1 cut(s) 665
BpuMI CCSGG 2 cut(s) 473, 521
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 129, 629
BsaJI CCNNGG 6 cut(s) 249, 299, 416, 439, 465, 567
BsaXI ACNNNNNCTCC 8 cut(s) 217, 247, 518, 548, 713, 743, 792, 822
Bsc4I CCNNNNNNNGG 8 cut(s) 211, 255, 284, 421, 471, 478, 683, 747
Bse118I RCCGGY 3 cut(s) 171, 421, 562
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 4 cut(s) 250, 256, 418, 569
BseDI CCNNGG 6 cut(s) 249, 299, 416, 439, 465, 567
BseGI GGATG 3 cut(s) 201, 576, 705
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 8 cut(s) 211, 255, 284, 421, 471, 478, 683, 747
BseSI GKGCMC 2 cut(s) 338, 678
BseXI GCAGC 2 cut(s) 180, 741
BseYI CCCAGC 2 cut(s) 337, 504
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 637
BshFI GGCC 6 cut(s) 254, 270, 421, 503, 566, 676
BshNI GGYRCC 2 cut(s) 333, 780
BsiHKAI GWGCWC 1 cut(s) 767
BsiHKCI CYCGRG 3 cut(s) 580, 746, 758
BsiSI CCGG 8 cut(s) 96, 172, 422, 472, 509, 521, 563, 778
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 8 cut(s) 211, 255, 284, 421, 471, 478, 683, 747
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 825
BsnI GGCC 6 cut(s) 254, 270, 421, 503, 566, 676
BsoBI CYCGRG 3 cut(s) 580, 746, 758
Bsp120I GGGCCC 1 cut(s) 674
Bsp1286I GDGCHC 7 cut(s) 8, 290, 338, 516, 653, 678, 767
Bsp143I GATC 6 cut(s) 46, 144, 541, 573, 742, 825
BspANI GGCC 6 cut(s) 254, 270, 421, 503, 566, 676
BspFNI CGCG 1 cut(s) 637
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 9 cut(s) 7, 100, 335, 372, 513, 561, 676, 782, 813
BspMI ACCTGC 1 cut(s) 340
BspT107I GGYRCC 2 cut(s) 333, 780
BsrBI CCGCTC 2 cut(s) 368, 669
BsrFI RCCGGY 3 cut(s) 171, 421, 562
BssAI RCCGGY 3 cut(s) 171, 421, 562
BssECI CCNNGG 6 cut(s) 249, 299, 416, 439, 465, 567
BssMI GATC 6 cut(s) 46, 144, 541, 573, 742, 825
BssNI GRCGYC 2 cut(s) 129, 629
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 4 cut(s) 250, 256, 418, 569
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 2 cut(s) 182, 403
BstACI GRCGYC 2 cut(s) 129, 629
BstC8I GCNNGC 4 cut(s) 173, 177, 423, 564
BstDSI CCRYGG 1 cut(s) 439
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 3 cut(s) 201, 576, 705
BstFNI CGCG 1 cut(s) 637
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 2 cut(s) 446, 587
BstKTI GATC 6 cut(s) 49, 147, 544, 576, 745, 828
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 6 cut(s) 46, 144, 541, 573, 742, 825
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNI CCWGG 4 cut(s) 250, 256, 418, 569
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 6 cut(s) 248, 254, 416, 471, 519, 567
BstSLI GKGCMC 2 cut(s) 338, 678
BstUI CGCG 1 cut(s) 637
BstV1I GCAGC 2 cut(s) 180, 741
BstX2I RGATCY 1 cut(s) 825
BstYI RGATCY 1 cut(s) 825
BsuRI GGCC 6 cut(s) 254, 270, 421, 503, 566, 676
BtgI CCRYGG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 691
BtsCI GGATG 3 cut(s) 201, 576, 705
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 4 cut(s) 173, 177, 423, 564
CciI TCATGA 1 cut(s) 33
CfoI GCGC 2 cut(s) 446, 587
Cfr10I RCCGGY 3 cut(s) 171, 421, 562
Cfr13I GGNCC 8 cut(s) 252, 370, 469, 502, 565, 674, 675, 812
Csp6I GTAC 1 cut(s) 265
CviAII CATG 2 cut(s) 34, 619
CviQI GTAC 1 cut(s) 265
DpnI GATC 6 cut(s) 48, 146, 543, 575, 744, 827
DpnII GATC 6 cut(s) 46, 144, 541, 573, 742, 825
EaeI YGGCCR 2 cut(s) 268, 419
Eam1104I CTCTTC 2 cut(s) 182, 403
EarI CTCTTC 2 cut(s) 182, 403
EciI GGCGGA 3 cut(s) 225, 274, 813
Eco130I CCWWGG 1 cut(s) 299
Eco24I GRGCYC 5 cut(s) 8, 290, 516, 653, 678
Eco47I GGWCC 3 cut(s) 370, 469, 812
Eco88I CYCGRG 3 cut(s) 580, 746, 758
Eco91I GGTNACC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 388
EcoRII CCWGG 4 cut(s) 248, 254, 416, 567
EcoT14I CCWWGG 1 cut(s) 299
EcoT38I GRGCYC 5 cut(s) 8, 290, 516, 653, 678
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 2 cut(s) 37, 622
FaiI YATR 4 cut(s) 35, 182, 380, 620
FaqI GGGAC 1 cut(s) 825
FatI CATG 2 cut(s) 33, 618
FauI CCCGC 3 cut(s) 124, 630, 688
FbaI TGATCA 2 cut(s) 46, 573
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 7 cut(s) 169, 498, 501, 550, 588, 612, 755
FokI GGATG 3 cut(s) 188, 563, 712
FriOI GRGCYC 5 cut(s) 8, 290, 516, 653, 678
Fsp4HI GCNGC 7 cut(s) 169, 498, 501, 550, 588, 612, 755
FspBI CTAG 2 cut(s) 300, 429
GlaI GCGC 2 cut(s) 445, 586
GluI GCNGC 7 cut(s) 169, 498, 501, 550, 588, 612, 755
GsaI CCCAGC 2 cut(s) 341, 508
GsuI CTGGAG 1 cut(s) 665
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 6 cut(s) 254, 270, 421, 503, 566, 676
HapII CCGG 8 cut(s) 96, 172, 422, 472, 509, 521, 563, 778
HhaI GCGC 2 cut(s) 446, 587
Hin1I GRCGYC 2 cut(s) 129, 629
Hin1II CATG 2 cut(s) 37, 622
Hin6I GCGC 2 cut(s) 444, 585
HinP1I GCGC 2 cut(s) 444, 585
HinfI GANTC 3 cut(s) 192, 245, 728
HpaII CCGG 8 cut(s) 96, 172, 422, 472, 509, 521, 563, 778
HphI GGTGA 4 cut(s) 282, 382, 583, 698
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 4 cut(s) 244, 403, 794, 825
Hpy188III TCNNGA 5 cut(s) 34, 50, 429, 580, 709
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 8 cut(s) 56, 131, 134, 557, 596, 631, 634, 637
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 3 cut(s) 129, 629, 690
HpyCH4V TGCA 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpySE526I ACGT 3 cut(s) 129, 629, 690
Hsp92I GRCGYC 2 cut(s) 129, 629
Hsp92II CATG 2 cut(s) 37, 622
HspAI GCGC 2 cut(s) 444, 585
KroI GCCGGC 3 cut(s) 171, 421, 562
KroNI GCCGGC 3 cut(s) 173, 423, 564
Ksp22I TGATCA 2 cut(s) 46, 573
Kzo9I GATC 6 cut(s) 46, 144, 541, 573, 742, 825
LmnI GCTCC 6 cut(s) 11, 98, 104, 511, 559, 751
Lsp1109I GCAGC 2 cut(s) 180, 741
MaeI CTAG 2 cut(s) 300, 429
MaeII ACGT 3 cut(s) 129, 629, 690
MaeIII GTNAC 3 cut(s) 221, 388, 686
MalI GATC 6 cut(s) 48, 146, 543, 575, 744, 827
MbiI CCGCTC 2 cut(s) 368, 669
MboI GATC 6 cut(s) 46, 144, 541, 573, 742, 825
MboII GAAGA 3 cut(s) 10, 199, 390
MflI RGATCY 1 cut(s) 825
MhlI GDGCHC 7 cut(s) 8, 290, 338, 516, 653, 678, 767
MlyI GAGTC 2 cut(s) 201, 737
MmeI TCCRAC 1 cut(s) 772
MroNI GCCGGC 3 cut(s) 171, 421, 562
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 8 cut(s) 96, 172, 422, 472, 509, 521, 563, 778
MspR9I CCNGG 6 cut(s) 250, 256, 418, 473, 521, 569
MvaI CCWGG 4 cut(s) 250, 256, 418, 569
MvnI CGCG 1 cut(s) 637
MwoI GCNNNNNNNGC 1 cut(s) 441
NaeI GCCGGC 3 cut(s) 173, 423, 564
NciI CCSGG 2 cut(s) 473, 521
NdeII GATC 6 cut(s) 46, 144, 541, 573, 742, 825
NgoMIV GCCGGC 3 cut(s) 171, 421, 562
NlaIII CATG 2 cut(s) 37, 622
NlaIV GGNNCC 9 cut(s) 7, 100, 335, 372, 513, 561, 676, 782, 813
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 388, 686
OliI CACNNNNGTG 1 cut(s) 786
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 340
PdiI GCCGGC 3 cut(s) 173, 423, 564
PfeI GAWTC 1 cut(s) 245
PflFI GACNNNGTC 2 cut(s) 131, 631
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 7 cut(s) 170, 499, 502, 551, 589, 613, 756
PleI GAGTC 2 cut(s) 200, 736
PpsI GAGTC 2 cut(s) 200, 736
Psp6I CCWGG 4 cut(s) 248, 254, 416, 567
PspEI GGTNACC 1 cut(s) 388
PspFI CCCAGC 2 cut(s) 337, 504
PspGI CCWGG 4 cut(s) 248, 254, 416, 567
PspN4I GGNNCC 9 cut(s) 7, 100, 335, 372, 513, 561, 676, 782, 813
PspOMI GGGCCC 1 cut(s) 674
PspPI GGNCC 8 cut(s) 252, 370, 469, 502, 565, 674, 675, 812
PsuI RGATCY 1 cut(s) 825
PsyI GACNNNGTC 2 cut(s) 131, 631
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 786
SatI GCNGC 7 cut(s) 169, 498, 501, 550, 588, 612, 755
Sau3AI GATC 6 cut(s) 46, 144, 541, 573, 742, 825
Sau96I GGNCC 8 cut(s) 252, 370, 469, 502, 565, 674, 675, 812
SchI GAGTC 2 cut(s) 201, 737
ScrFI CCNGG 6 cut(s) 250, 256, 418, 473, 521, 569
SduI GDGCHC 7 cut(s) 8, 290, 338, 516, 653, 678, 767
SinI GGWCC 3 cut(s) 370, 469, 812
SmiMI CAYNNNNRTG 1 cut(s) 786
SspMI CTAG 2 cut(s) 300, 429
StyD4I CCNGG 6 cut(s) 248, 254, 416, 471, 519, 567
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 3 cut(s) 132, 632, 693
TauI GCSGC 5 cut(s) 500, 503, 552, 590, 614
TfiI GAWTC 1 cut(s) 245
TseFI GTSAC 2 cut(s) 388, 686
TseI GCWGC 2 cut(s) 168, 754
Tsp45I GTSAC 2 cut(s) 388, 686
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 677
Tth111I GACNNNGTC 2 cut(s) 131, 631
VpaK11BI GGWCC 3 cut(s) 370, 469, 812
XbaI TCTAGA 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 300, 429
ZraI GACGTC 2 cut(s) 130, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.