pycom10g23050
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011269
Physical Location & Seq
Reverse (-)
25289433 .. 25290660
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23050.1

Sequence Viewer

Length: 810 bp
ATGGACCCCACATTCTTCAGCAGCAACAGCAAACAGATTATGAGCGATCAGGAGGAGAATGACAACACCACCTCCTCCACGCCGGAAAACAGCTCAGACTCTCCTCCGCCGTCTACCAATTTCAGCGACTACTCCAAGATCACCACTTCCACCTCTTCCCCCAAAAAAAGTGCTCGGCGAGCTATACAGAAACGAGTGGTGTCGGTACCCATAAAAAATGAGAACAGCAACACTCCGCCGCCTTCAGATTCTTGGGCTTGGAGAAAGTACGGCCAGAAACCCATCAAAGGCTCCCCTTATCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGATGCCCGGCGAGGAAACAAGTGGAAAGAAGCCGCGTGGACCCCACCATGCTGGTCATCACCTACTCCGCCGAACACAACCACCCTTGGCCCGCCTCTCGCAACCACCACAACAACCGCAGCATTTCAAATCCCGCCGCTGGAACCAAACCCGGCCTCACCCCGGAGGCCCAGCAACCCGACCACCAAGACCAAGAGCAAGACCCGAGGTTCGCGGATCTAAACCACGAGTCGCTTCTCGTAAACGACGATTACGTCTGGTTCGCTGACATGGAGACGACGTCATCGACGGTGCTCGAGAGCCCTATCTTCGCCGAGAGCGGCGGCTCCGTCGGGTCCGACTCCGGGGACATGGCGGAAATGGTGTTCCCGATGGGAGAAGAGGACGAGTCTCTATTCGCCGATCTCGGCGAGCTGCCGGAGTATTCCCTCGTGTTTCGACACCGCGGCGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

270

Amino Acids

29.79

Weight (kDa)

5.19

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 83 - 140 3e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 587
AatII GACGTC 1 cut(s) 617
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 113
AccII CGCG 3 cut(s) 369, 548, 780
AclWI GGATC 1 cut(s) 558
AcoI YGGCCR 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 2 cut(s) 207, 269
AfiI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 678, 784
AgsI TTSAA 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 683, 715
AluBI AGCT 5 cut(s) 93, 182, 327, 748, 797
AluI AGCT 5 cut(s) 93, 182, 327, 748, 797
Alw21I GWGCWC 2 cut(s) 175, 630
Alw26I GTCTC 2 cut(s) 602, 729
AlwI GGATC 1 cut(s) 558
Ama87I CYCGRG 2 cut(s) 538, 629
AoxI GGCC 4 cut(s) 271, 422, 487, 501
ApeKI GCWGC 3 cut(s) 21, 453, 748
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp718I GGTACC 1 cut(s) 205
AspS9I GGNCC 6 cut(s) 4, 373, 423, 502, 669, 788
AsuC2I CCSGG 4 cut(s) 341, 486, 497, 679
AsuHPI GGTGA 3 cut(s) 133, 385, 484
AvaI CYCGRG 2 cut(s) 538, 629
AvaII GGWCC 4 cut(s) 4, 373, 669, 788
BanI GGYRCC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 638
BauI CACGAG 2 cut(s) 560, 764
Bbv12I GWGCWC 2 cut(s) 175, 630
BbvI GCAGC 3 cut(s) 33, 465, 735
BccI CCATC 2 cut(s) 290, 700
BceAI ACGGC 2 cut(s) 94, 286
BcnI CCSGG 4 cut(s) 341, 486, 497, 679
BcoDI GTCTC 2 cut(s) 602, 729
BglII AGATCT 1 cut(s) 801
BisI GCNGC 9 cut(s) 22, 239, 367, 454, 471, 655, 658, 749, 781
BlsI GCNGC 9 cut(s) 23, 240, 368, 455, 472, 656, 659, 750, 782
Bme1390I CCNGG 4 cut(s) 341, 486, 497, 679
Bme18I GGWCC 4 cut(s) 4, 373, 669, 788
BmeT110I CYCGRG 2 cut(s) 538, 629
BmgT120I GGNCC 6 cut(s) 4, 373, 423, 502, 669, 788
BmiI GGNNCC 8 cut(s) 6, 207, 292, 375, 478, 661, 670, 789
BmrFI CCNGG 4 cut(s) 341, 486, 497, 679
BmsI GCATC 1 cut(s) 326
BpuMI CCSGG 4 cut(s) 341, 486, 497, 679
BsaHI GRCGYC 1 cut(s) 614
BsaJI CCNNGG 5 cut(s) 419, 495, 539, 678, 778
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 599, 629
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 473, 496, 678, 784
BseDI CCNNGG 5 cut(s) 419, 495, 539, 678, 778
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 678, 784
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 3 cut(s) 64, 68, 93
BseXI GCAGC 3 cut(s) 33, 465, 735
BseYI CCCAGC 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 3 cut(s) 369, 548, 780
BshFI GGCC 4 cut(s) 273, 424, 489, 503
BshNI GGYRCC 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 175, 630
BsiHKCI CYCGRG 2 cut(s) 538, 629
BsiSI CCGG 6 cut(s) 83, 341, 486, 497, 678, 752
BslFI GGGAC 2 cut(s) 695, 801
BslI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 678, 784
BsmAI GTCTC 2 cut(s) 602, 729
BsmBI CGTCTC 1 cut(s) 602
BsmFI GGGAC 2 cut(s) 695, 801
BsnI GGCC 4 cut(s) 273, 424, 489, 503
BsoBI CYCGRG 2 cut(s) 538, 629
Bsp1286I GDGCHC 3 cut(s) 175, 630, 638
Bsp143I GATC 5 cut(s) 46, 138, 550, 736, 801
BspANI GGCC 4 cut(s) 273, 424, 489, 503
BspCNI CTCAG 1 cut(s) 107
BspFNI CGCG 3 cut(s) 369, 548, 780
BspLI GGNNCC 8 cut(s) 6, 207, 292, 375, 478, 661, 670, 789
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 654
BssECI CCNNGG 5 cut(s) 419, 495, 539, 678, 778
BssMI GATC 5 cut(s) 46, 138, 550, 736, 801
BssNI GRCGYC 1 cut(s) 614
BssSI CACGAG 2 cut(s) 560, 764
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 2 cut(s) 560, 764
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 2 cut(s) 160, 708
BstACI GRCGYC 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 180, 426, 746
BstDEI CTNAG 2 cut(s) 94, 798
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 3 cut(s) 369, 548, 780
BstKTI GATC 5 cut(s) 49, 141, 553, 739, 804
BstMAI GTCTC 2 cut(s) 602, 729
BstMBI GATC 5 cut(s) 46, 138, 550, 736, 801
BstMWI GCNNNNNNNGC 2 cut(s) 27, 179
BstSCI CCNGG 4 cut(s) 339, 484, 495, 677
BstUI CGCG 3 cut(s) 369, 548, 780
BstV1I GCAGC 3 cut(s) 33, 465, 735
BstX2I RGATCY 2 cut(s) 550, 801
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 550, 801
BsuRI GGCC 4 cut(s) 273, 424, 489, 503
BtgI CCRYGG 1 cut(s) 778
BtsCI GGATG 1 cut(s) 341
Cac8I GCNNGC 3 cut(s) 180, 426, 746
Cfr13I GGNCC 6 cut(s) 4, 373, 423, 502, 669, 788
Cfr42I CCGCGG 1 cut(s) 781
Csp6I GTAC 2 cut(s) 206, 268
CviAII CATG 3 cut(s) 382, 604, 685
CviQI GTAC 2 cut(s) 206, 268
DdeI CTNAG 2 cut(s) 94, 798
DpnI GATC 5 cut(s) 48, 140, 552, 738, 803
DpnII GATC 5 cut(s) 46, 138, 550, 736, 801
DrdI GACNNNNNNGTC 1 cut(s) 587
DseDI GACNNNNNNGTC 1 cut(s) 587
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 160, 708
EarI CTCTTC 2 cut(s) 160, 708
EciI GGCGGA 4 cut(s) 96, 225, 391, 704
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 638
Eco47I GGWCC 4 cut(s) 4, 373, 669, 788
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 2 cut(s) 538, 629
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 638
ErhI CCWWGG 1 cut(s) 419
Esp3I CGTCTC 1 cut(s) 602
FaeI CATG 3 cut(s) 385, 607, 688
FaiI YATR 6 cut(s) 41, 185, 212, 383, 605, 686
FaqI GGGAC 2 cut(s) 695, 801
FatI CATG 3 cut(s) 381, 603, 684
FauI CCCGC 2 cut(s) 433, 475
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 9 cut(s) 22, 239, 367, 454, 471, 655, 658, 749, 781
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 9 cut(s) 22, 239, 367, 454, 471, 655, 658, 749, 781
GluI GCNGC 9 cut(s) 22, 239, 367, 454, 471, 655, 658, 749, 781
GsaI CCCAGC 1 cut(s) 508
HaeIII GGCC 4 cut(s) 273, 424, 489, 503
HapII CCGG 6 cut(s) 83, 341, 486, 497, 678, 752
Hin1I GRCGYC 1 cut(s) 614
Hin1II CATG 3 cut(s) 385, 607, 688
HinfI GANTC 5 cut(s) 98, 248, 563, 674, 722
HpaII CCGG 6 cut(s) 83, 341, 486, 497, 678, 752
HphI GGTGA 3 cut(s) 133, 385, 484
Hpy166II GTNNAC 3 cut(s) 114, 373, 577
Hpy188I TCNGA 4 cut(s) 97, 247, 673, 801
Hpy188III TCNNGA 3 cut(s) 50, 631, 703
Hpy8I GTNNAC 3 cut(s) 114, 373, 577
Hpy99I CGWCG 4 cut(s) 584, 616, 625, 668
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 2 cut(s) 588, 614
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 179
HpyF3I CTNAG 2 cut(s) 94, 798
HpySE526I ACGT 2 cut(s) 588, 614
Hsp92I GRCGYC 1 cut(s) 614
Hsp92II CATG 3 cut(s) 385, 607, 688
KpnI GGTACC 1 cut(s) 209
KspI CCGCGG 1 cut(s) 781
Kzo9I GATC 5 cut(s) 46, 138, 550, 736, 801
LmnI GCTCC 2 cut(s) 296, 665
Lsp1109I GCAGC 3 cut(s) 33, 465, 735
LweI GCATC 1 cut(s) 326
MaeII ACGT 2 cut(s) 588, 614
MalI GATC 5 cut(s) 48, 140, 552, 738, 803
MbiI CCGCTC 1 cut(s) 654
MboI GATC 5 cut(s) 46, 138, 550, 736, 801
MboII GAAGA 4 cut(s) 7, 147, 634, 725
MflI RGATCY 2 cut(s) 550, 801
MhlI GDGCHC 3 cut(s) 175, 630, 638
MluCI AATT 1 cut(s) 118
MlyI GAGTC 4 cut(s) 92, 572, 668, 731
MmeI TCCRAC 1 cut(s) 696
MspA1I CMGCKG 2 cut(s) 473, 780
MspI CCGG 6 cut(s) 83, 341, 486, 497, 678, 752
MspR9I CCNGG 4 cut(s) 341, 486, 497, 679
MvnI CGCG 3 cut(s) 369, 548, 780
MwoI GCNNNNNNNGC 2 cut(s) 27, 179
NciI CCSGG 4 cut(s) 341, 486, 497, 679
NdeII GATC 5 cut(s) 46, 138, 550, 736, 801
NlaIII CATG 3 cut(s) 385, 607, 688
NlaIV GGNNCC 8 cut(s) 6, 207, 292, 375, 478, 661, 670, 789
NmeAIII GCCGAG 3 cut(s) 154, 673, 720
PaeR7I CTCGAG 1 cut(s) 629
PcsI WCGNNNNNNNCGW 3 cut(s) 579, 585, 617
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 613
PkrI GCNGC 9 cut(s) 23, 240, 368, 455, 472, 656, 659, 750, 782
PleI GAGTC 4 cut(s) 92, 571, 668, 730
PpsI GAGTC 4 cut(s) 92, 571, 668, 730
PspFI CCCAGC 1 cut(s) 504
PspN4I GGNNCC 8 cut(s) 6, 207, 292, 375, 478, 661, 670, 789
PspPI GGNCC 6 cut(s) 4, 373, 423, 502, 669, 788
PsuI RGATCY 2 cut(s) 550, 801
PsyI GACNNNGTC 1 cut(s) 613
RsaI GTAC 2 cut(s) 207, 269
RsaNI GTAC 2 cut(s) 206, 268
SacII CCGCGG 1 cut(s) 781
SatI GCNGC 9 cut(s) 22, 239, 367, 454, 471, 655, 658, 749, 781
Sau3AI GATC 5 cut(s) 46, 138, 550, 736, 801
Sau96I GGNCC 6 cut(s) 4, 373, 423, 502, 669, 788
SchI GAGTC 4 cut(s) 92, 572, 668, 731
ScrFI CCNGG 4 cut(s) 341, 486, 497, 679
SduI GDGCHC 3 cut(s) 175, 630, 638
SfaNI GCATC 1 cut(s) 326
Sfr274I CTCGAG 1 cut(s) 629
Sfr303I CCGCGG 1 cut(s) 781
SgrBI CCGCGG 1 cut(s) 781
SinI GGWCC 4 cut(s) 4, 373, 669, 788
SlaI CTCGAG 1 cut(s) 629
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 1 cut(s) 118
StyD4I CCNGG 4 cut(s) 339, 484, 495, 677
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 2 cut(s) 591, 617
TaqI TCGA 3 cut(s) 620, 630, 772
TasI AATT 1 cut(s) 118
TauI GCSGC 6 cut(s) 241, 369, 473, 657, 660, 783
TfiI GAWTC 1 cut(s) 248
TseI GCWGC 3 cut(s) 21, 453, 748
TspGWI ACGGA 1 cut(s) 652
Tth111I GACNNNGTC 1 cut(s) 613
VpaK11BI GGWCC 4 cut(s) 4, 373, 669, 788
XhoI CTCGAG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 113
ZraI GACGTC 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.