Rh5AG089500
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011269
Physical Location & Seq
Forward (+)
7990056 .. 7992117
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG089500.1

Sequence Viewer

Length: 723 bp
ATGTATGTAGTCAATTCGATCACCAATATGAAATTAACGTTTAAATTTCTTGTTTACAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAATCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCACGAAACCCGGGATCAAAACCGAGACCCGGGAAGAGGAGACGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGAGGCGGTTCGCGGCGGATCTGGGCGGCGGCGACGAGGAGTCGTCGCTGGTGATCATCCCGAGCGTCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCCATTTTCGCTGAGAGCGGCGGTGGGGCCGACACGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCGCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGTGTGGGAGGAGGAGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

240

Amino Acids

26.43

Weight (kDa)

5.63

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 47 - 104 5.8e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
AatII GACGTC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 672
AccBSI CCGCTC 1 cut(s) 558
AccII CGCG 4 cut(s) 261, 425, 503, 526
AclI AACGTT 1 cut(s) 38
AclWI GGATC 2 cut(s) 359, 438
AcoI YGGCCR 4 cut(s) 163, 314, 384, 388
AcsI RAATTY 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 75, 161
AfiI CCNNNNNNNGG 7 cut(s) 103, 136, 179, 316, 366, 373, 682
AhdI GACNNNNNGTC 1 cut(s) 451
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 2 cut(s) 149, 311
AleI CACNNNNGTG 2 cut(s) 278, 678
AloI GAACNNNNNNTCC 2 cut(s) 404, 436
AluBI AGCT 2 cut(s) 219, 710
AluI AGCT 2 cut(s) 219, 710
Alw21I GWGCWC 2 cut(s) 303, 659
Alw26I GTCTC 2 cut(s) 356, 371
AlwI GGATC 2 cut(s) 359, 438
Ama87I CYCGRG 4 cut(s) 347, 366, 472, 650
AoxI GGCC 6 cut(s) 147, 163, 314, 384, 388, 567
ApoI RAATTY 1 cut(s) 44
AspS9I GGNCC 4 cut(s) 147, 265, 567, 701
AsuC2I CCSGG 5 cut(s) 348, 349, 367, 368, 407
AsuHPI GGTGA 5 cut(s) 13, 177, 277, 292, 475
AvaI CYCGRG 4 cut(s) 347, 366, 472, 650
AvaII GGWCC 2 cut(s) 265, 701
BanI GGYRCC 1 cut(s) 672
BanII GRGCYC 2 cut(s) 185, 542
Bbv12I GWGCWC 2 cut(s) 303, 659
BccI CCATC 3 cut(s) 182, 586, 598
BceAI ACGGC 5 cut(s) 118, 178, 352, 396, 663
BciT130I CCWGG 2 cut(s) 151, 313
BclI TGATCA 2 cut(s) 282, 465
BcnI CCSGG 5 cut(s) 348, 349, 367, 368, 407
BcoDI GTCTC 2 cut(s) 356, 371
BfaI CTAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 336
BglII AGATCT 1 cut(s) 714
BisI GCNGC 6 cut(s) 259, 384, 426, 439, 442, 559
BlsI GCNGC 6 cut(s) 260, 385, 427, 440, 443, 560
Bme1390I CCNGG 7 cut(s) 151, 313, 348, 349, 367, 368, 407
Bme18I GGWCC 2 cut(s) 265, 701
BmeRI GACNNNNNGTC 1 cut(s) 451
BmeT110I CYCGRG 4 cut(s) 347, 366, 472, 650
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 4 cut(s) 147, 265, 567, 701
BmiI GGNNCC 5 cut(s) 267, 399, 568, 674, 702
BmrFI CCNGG 7 cut(s) 151, 313, 348, 349, 367, 368, 407
BpmI CTGGAG 2 cut(s) 172, 554
BpuMI CCSGG 5 cut(s) 348, 349, 367, 368, 407
BsaHI GRCGYC 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 311, 334, 347, 366
BsaXI ACNNNNNCTCC 6 cut(s) 109, 139, 404, 434, 681, 711
Bsc4I CCNNNNNNNGG 7 cut(s) 103, 136, 179, 316, 366, 373, 682
Bse118I RCCGGY 3 cut(s) 63, 316, 386
BseBI CCWGG 2 cut(s) 151, 313
BseDI CCNNGG 4 cut(s) 311, 334, 347, 366
BseGI GGATG 3 cut(s) 468, 583, 597
BseLI CCNNNNNNNGG 7 cut(s) 103, 136, 179, 316, 366, 373, 682
BseMII CTCAG 1 cut(s) 543
BseRI GAGGAG 5 cut(s) 389, 425, 464, 702, 705
BseX3I CGGCCG 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 232
Bsh1236I CGCG 4 cut(s) 261, 425, 503, 526
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 6 cut(s) 149, 165, 316, 386, 390, 569
BshNI GGYRCC 1 cut(s) 672
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 303, 659
BsiHKCI CYCGRG 4 cut(s) 347, 366, 472, 650
BsiSI CCGG 7 cut(s) 64, 317, 348, 367, 387, 395, 407
BslFI GGGAC 1 cut(s) 714
BslI CCNNNNNNNGG 7 cut(s) 103, 136, 179, 316, 366, 373, 682
BsmAI GTCTC 2 cut(s) 356, 371
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 714
BsnI GGCC 6 cut(s) 149, 165, 316, 386, 390, 569
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 4 cut(s) 347, 366, 472, 650
Bsp1286I GDGCHC 4 cut(s) 185, 303, 542, 659
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
BspANI GGCC 6 cut(s) 149, 165, 316, 386, 390, 569
BspCNI CTCAG 1 cut(s) 544
BspFNI CGCG 4 cut(s) 261, 425, 503, 526
BspLI GGNNCC 5 cut(s) 267, 399, 568, 674, 702
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 2 cut(s) 359, 438
BspT107I GGYRCC 1 cut(s) 672
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 558
BsrFI RCCGGY 3 cut(s) 63, 316, 386
BsrGI TGTACA 1 cut(s) 73
BssAI RCCGGY 3 cut(s) 63, 316, 386
BssECI CCNNGG 4 cut(s) 311, 334, 347, 366
BssMI GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
BssNI GRCGYC 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 151, 313
Bst4CI ACNGT 2 cut(s) 59, 113
Bst6I CTCTTC 3 cut(s) 74, 366, 606
BstACI GRCGYC 1 cut(s) 518
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 6 cut(s) 65, 69, 318, 388, 392, 659
BstDEI CTNAG 2 cut(s) 552, 711
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 3 cut(s) 468, 583, 597
BstFNI CGCG 4 cut(s) 261, 425, 503, 526
BstKTI GATC 7 cut(s) 21, 285, 354, 433, 468, 637, 717
BstMAI GTCTC 2 cut(s) 356, 371
BstMBI GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNI CCWGG 2 cut(s) 151, 313
BstSCI CCNGG 7 cut(s) 149, 311, 346, 347, 365, 366, 405
BstUI CGCG 4 cut(s) 261, 425, 503, 526
BstX2I RGATCY 2 cut(s) 430, 714
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 2 cut(s) 430, 714
BstZI CGGCCG 1 cut(s) 384
BsuRI GGCC 6 cut(s) 149, 165, 316, 386, 390, 569
BtgI CCRYGG 1 cut(s) 334
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 3 cut(s) 468, 583, 597
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 6 cut(s) 65, 69, 318, 388, 392, 659
Cfr10I RCCGGY 3 cut(s) 63, 316, 386
Cfr13I GGNCC 4 cut(s) 147, 265, 567, 701
Cfr9I CCCGGG 2 cut(s) 347, 366
CseI GACGC 1 cut(s) 466
Csp6I GTAC 2 cut(s) 74, 160
CviAII CATG 2 cut(s) 144, 508
CviQI GTAC 2 cut(s) 74, 160
DdeI CTNAG 2 cut(s) 552, 711
DpnI GATC 7 cut(s) 20, 284, 353, 432, 467, 636, 716
DpnII GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
DraI TTTAAA 1 cut(s) 43
DriI GACNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 4 cut(s) 163, 314, 384, 388
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 3 cut(s) 74, 366, 606
Eam1105I GACNNNNNGTC 1 cut(s) 451
EarI CTCTTC 3 cut(s) 74, 366, 606
EciI GGCGGA 1 cut(s) 443
EclXI CGGCCG 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 185, 542
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 2 cut(s) 265, 701
Eco52I CGGCCG 1 cut(s) 384
Eco88I CYCGRG 4 cut(s) 347, 366, 472, 650
EcoRII CCWGG 2 cut(s) 149, 311
EcoT38I GRGCYC 2 cut(s) 185, 542
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 147, 511
FaiI YATR 4 cut(s) 6, 29, 145, 509
FaqI GGGAC 1 cut(s) 714
FatI CATG 2 cut(s) 143, 507
FauI CCCGC 4 cut(s) 60, 138, 519, 580
FbaI TGATCA 2 cut(s) 282, 465
Fnu4HI GCNGC 6 cut(s) 259, 384, 426, 439, 442, 559
FokI GGATG 3 cut(s) 455, 590, 604
FriOI GRGCYC 2 cut(s) 185, 542
FseI GGCCGGCC 1 cut(s) 390
Fsp4HI GCNGC 6 cut(s) 259, 384, 426, 439, 442, 559
FspBI CTAG 1 cut(s) 324
GluI GCNGC 6 cut(s) 259, 384, 426, 439, 442, 559
GsuI CTGGAG 2 cut(s) 172, 554
HaeIII GGCC 6 cut(s) 149, 165, 316, 386, 390, 569
HapII CCGG 7 cut(s) 64, 317, 348, 367, 387, 395, 407
HgaI GACGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 2 cut(s) 147, 511
HinfI GANTC 3 cut(s) 140, 452, 620
HpaII CCGG 7 cut(s) 64, 317, 348, 367, 387, 395, 407
HphI GGTGA 5 cut(s) 13, 177, 277, 292, 475
Hpy166II GTNNAC 3 cut(s) 55, 265, 484
Hpy188I TCNGA 3 cut(s) 139, 298, 714
Hpy188III TCNNGA 3 cut(s) 324, 472, 601
Hpy8I GTNNAC 3 cut(s) 55, 265, 484
Hpy99I CGWCG 7 cut(s) 139, 449, 460, 482, 520, 523, 526
HpyCH4III ACNGT 2 cut(s) 59, 113
HpyCH4IV ACGT 3 cut(s) 38, 518, 582
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 552, 711
HpySE526I ACGT 3 cut(s) 38, 518, 582
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 2 cut(s) 147, 511
KroI GCCGGC 3 cut(s) 63, 316, 386
KroNI GCCGGC 3 cut(s) 65, 318, 388
Ksp22I TGATCA 2 cut(s) 282, 465
Kzo9I GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
MaeI CTAG 1 cut(s) 324
MaeII ACGT 3 cut(s) 38, 518, 582
MaeIII GTNAC 1 cut(s) 113
MalI GATC 7 cut(s) 20, 284, 353, 432, 467, 636, 716
MbiI CCGCTC 1 cut(s) 558
MboI GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
MboII GAAGA 3 cut(s) 91, 383, 623
MflI RGATCY 2 cut(s) 430, 714
MhlI GDGCHC 4 cut(s) 185, 303, 542, 659
MluCI AATT 3 cut(s) 13, 32, 44
MlyI GAGTC 2 cut(s) 461, 629
MmeI TCCRAC 1 cut(s) 117
MroNI GCCGGC 3 cut(s) 63, 316, 386
MseI TTAA 2 cut(s) 35, 42
MslI CAYNNNNRTG 3 cut(s) 26, 278, 678
MspI CCGG 7 cut(s) 64, 317, 348, 367, 387, 395, 407
MspR9I CCNGG 7 cut(s) 151, 313, 348, 349, 367, 368, 407
MvaI CCWGG 2 cut(s) 151, 313
MvnI CGCG 4 cut(s) 261, 425, 503, 526
MwoI GCNNNNNNNGC 1 cut(s) 336
NaeI GCCGGC 3 cut(s) 65, 318, 388
NciI CCSGG 5 cut(s) 348, 349, 367, 368, 407
NdeII GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
NgoMIV GCCGGC 3 cut(s) 63, 316, 386
NlaIII CATG 2 cut(s) 147, 511
NlaIV GGNNCC 5 cut(s) 267, 399, 568, 674, 702
OliI CACNNNNGTG 2 cut(s) 278, 678
PaqCI CACCTGC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 676
PdiI GCCGGC 3 cut(s) 65, 318, 388
PfeI GAWTC 1 cut(s) 140
PflFI GACNNNGTC 1 cut(s) 520
PkrI GCNGC 6 cut(s) 260, 385, 427, 440, 443, 560
PleI GAGTC 2 cut(s) 460, 628
PpsI GAGTC 2 cut(s) 460, 628
Psp1406I AACGTT 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 149, 311
PspGI CCWGG 2 cut(s) 149, 311
PspN4I GGNNCC 5 cut(s) 267, 399, 568, 674, 702
PspPI GGNCC 4 cut(s) 147, 265, 567, 701
PsuI RGATCY 2 cut(s) 430, 714
PsyI GACNNNGTC 1 cut(s) 520
RigI GGCCGGCC 1 cut(s) 390
RsaI GTAC 2 cut(s) 75, 161
RsaNI GTAC 2 cut(s) 74, 160
RseI CAYNNNNRTG 3 cut(s) 26, 278, 678
SaqAI TTAA 2 cut(s) 35, 42
SatI GCNGC 6 cut(s) 259, 384, 426, 439, 442, 559
Sau3AI GATC 7 cut(s) 18, 282, 351, 430, 465, 634, 714
Sau96I GGNCC 4 cut(s) 147, 265, 567, 701
SchI GAGTC 2 cut(s) 461, 629
ScrFI CCNGG 7 cut(s) 151, 313, 348, 349, 367, 368, 407
SduI GDGCHC 4 cut(s) 185, 303, 542, 659
SinI GGWCC 2 cut(s) 265, 701
SmaI CCCGGG 2 cut(s) 349, 368
SmiMI CAYNNNNRTG 3 cut(s) 26, 278, 678
Sse9I AATT 3 cut(s) 13, 32, 44
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 7 cut(s) 149, 311, 346, 347, 365, 366, 405
TaaI ACNGT 2 cut(s) 59, 113
TaiI ACGT 3 cut(s) 41, 521, 585
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 13, 32, 44
TatI WGTACW 1 cut(s) 73
TauI GCSGC 6 cut(s) 261, 386, 428, 441, 444, 561
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 35, 42
Tru9I TTAA 2 cut(s) 35, 42
TspDTI ATGAA 2 cut(s) 44, 132
TspGWI ACGGA 1 cut(s) 590
TspMI CCCGGG 2 cut(s) 347, 366
Tth111I GACNNNGTC 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 265, 701
XapI RAATTY 1 cut(s) 44
XbaI TCTAGA 1 cut(s) 323
XmaI CCCGGG 2 cut(s) 347, 366
XspI CTAG 1 cut(s) 324
ZraI GACGTC 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.