MD05G1295700.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011269
Physical Location & Seq
Reverse (-)
43023949 .. 43025466
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1295700.v1.1.491

Sequence Viewer

Length: 822 bp
ATGGACCCCACATTCTTCAGCAGCAACAGCAAACAGTTTCTGAGCGATCAGGAAGAGAATGACAACACCACCTCCTCCACGCCGGAAAACAGTTCAGACTCTCCTCCGCCGTCCACCAATTTCAGCGACTACCCCAAGATCACCACTTCCACCTCCTCCCCAAAAAAAAGTGCTCGGCGAGCAATACACAAACGAGTGGTGTCGGTCCCCATCAAAGGCGACAACAGCAACACTCCGCCGCCGTCCGATTCTTGGGCTTGGAGAAAGTACGGCCAAAAACCCATCAAAGGCTCACCTTATCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAAGGATGCCCTGCGAGGAAACAAGTAGAGAGAAGCCGCGTGGACCCCACCACGCTGGTCATCACCTACTCCTCCGAACACAACCACTCTTGGCCCGCCTCTCGCAACCACCACAACAACCACAGCAATTCAAGTTCCGCCGCCGCAACCAAACGCGGCCCCACCAAGACCGAAGCCCCGGAGGCCCAGCAACCCGACCACCAAGATCAAGACCAAGACTCTAGGTTCGCGGATCTAAACCACGAGTCGCTTATCGTAAACGACGAGTGCGTCTGGTTCCACGACATGGAGACCACGTCGTCGAGGGTGCTCGAGAGCCCCATTTTCGCCGAGAGCAGCAGCTCAGTCGGGTCCGACTCGGCGGACAAGGCGGCGATGGTGTTCCCCATGGGAGAGGAGGACGAGTCGCTCTTCGCCGATCTTGGCGAGCTGCCGGAGTGCTCTTTCGTGTTTAGGCACCGTGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

274

Amino Acids

30.2

Weight (kDa)

5.8

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 83 - 139 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 599, 628
AccB1I GGYRCC 1 cut(s) 786
AccB7I CCANNNNNTGG 1 cut(s) 616
AccII CGCG 3 cut(s) 369, 486, 560
AclWI GGATC 1 cut(s) 570
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 5 cut(s) 215, 252, 287, 616, 796
AgsI TTSAA 1 cut(s) 462
AjiI CACGTC 1 cut(s) 627
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 4 cut(s) 327, 672, 760, 809
AluI AGCT 4 cut(s) 327, 672, 760, 809
Alw21I GWGCWC 3 cut(s) 175, 642, 773
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 1 cut(s) 570
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 4 cut(s) 271, 422, 487, 513
ApeKI GCWGC 4 cut(s) 21, 666, 669, 760
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
AspS9I GGNCC 8 cut(s) 4, 205, 373, 423, 488, 514, 681, 800
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 133, 285, 385
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 5 cut(s) 4, 205, 373, 681, 800
BanI GGYRCC 1 cut(s) 786
BanII GRGCYC 1 cut(s) 650
BauI CACGAG 1 cut(s) 572
Bbv12I GWGCWC 3 cut(s) 175, 642, 773
BbvI GCAGC 4 cut(s) 33, 678, 681, 747
BccI CCATC 3 cut(s) 218, 290, 700
BceAI ACGGC 3 cut(s) 94, 226, 286
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 1 cut(s) 552
BglI GCCNNNNNGGC 1 cut(s) 512
BglII AGATCT 1 cut(s) 813
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 5 cut(s) 4, 205, 373, 681, 800
BmeT110I CYCGRG 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 627
BmgT120I GGNCC 8 cut(s) 4, 205, 373, 423, 488, 514, 681, 800
BmiI GGNNCC 8 cut(s) 6, 207, 375, 490, 608, 682, 788, 801
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 3 cut(s) 507, 717, 790
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 611, 641, 719, 749
Bsc4I CCNNNNNNNGG 5 cut(s) 215, 252, 287, 616, 796
BseDI CCNNGG 3 cut(s) 507, 717, 790
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 5 cut(s) 215, 252, 287, 616, 796
BseMII CTCAG 2 cut(s) 32, 687
BseRI GAGGAG 5 cut(s) 64, 93, 145, 391, 740
BseXI GCAGC 4 cut(s) 33, 678, 681, 747
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 3 cut(s) 369, 486, 560
BshFI GGCC 4 cut(s) 273, 424, 489, 515
BshNI GGYRCC 1 cut(s) 786
BsiHKAI GWGCWC 3 cut(s) 175, 642, 773
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 3 cut(s) 83, 509, 764
BslFI GGGAC 2 cut(s) 191, 813
BslI CCNNNNNNNGG 5 cut(s) 215, 252, 287, 616, 796
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 191, 813
BsnI GGCC 4 cut(s) 273, 424, 489, 515
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 4 cut(s) 175, 642, 650, 773
Bsp143I GATC 6 cut(s) 46, 138, 535, 562, 748, 813
Bsp19I CCATGG 1 cut(s) 717
BspANI GGCC 4 cut(s) 273, 424, 489, 515
BspCNI CTCAG 2 cut(s) 33, 686
BspFNI CGCG 3 cut(s) 369, 486, 560
BspLI GGNNCC 8 cut(s) 6, 207, 375, 490, 608, 682, 788, 801
BspPI GGATC 1 cut(s) 570
BspQI GCTCTTC 1 cut(s) 746
BspT107I GGYRCC 1 cut(s) 786
BspTNI GGTCTC 1 cut(s) 614
BssECI CCNNGG 3 cut(s) 507, 717, 790
BssMI GATC 6 cut(s) 46, 138, 535, 562, 748, 813
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 572
Bst4CI ACNGT 3 cut(s) 36, 92, 791
Bst6I CTCTTC 2 cut(s) 48, 746
BstC8I GCNNGC 3 cut(s) 180, 426, 758
BstDEI CTNAG 3 cut(s) 41, 673, 810
BstDSI CCRYGG 2 cut(s) 717, 790
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 3 cut(s) 369, 486, 560
BstKTI GATC 6 cut(s) 49, 141, 538, 565, 751, 816
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 6 cut(s) 46, 138, 535, 562, 748, 813
BstMWI GCNNNNNNNGC 5 cut(s) 27, 179, 225, 512, 698
BstSCI CCNGG 1 cut(s) 507
BstUI CGCG 3 cut(s) 369, 486, 560
BstV1I GCAGC 4 cut(s) 33, 678, 681, 747
BstX2I RGATCY 2 cut(s) 562, 813
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 562, 813
BsuRI GGCC 4 cut(s) 273, 424, 489, 515
BtgI CCRYGG 2 cut(s) 717, 790
BtgZI GCGATG 1 cut(s) 719
BtrI CACGTC 1 cut(s) 627
BtsCI GGATG 1 cut(s) 341
Cac8I GCNNGC 3 cut(s) 180, 426, 758
CaiI CAGNNNCTG 1 cut(s) 40
Cfr13I GGNCC 8 cut(s) 4, 205, 373, 423, 488, 514, 681, 800
CseI GACGC 1 cut(s) 589
Csp6I GTAC 1 cut(s) 268
CviAII CATG 2 cut(s) 616, 718
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 41, 673, 810
DpnI GATC 6 cut(s) 48, 140, 537, 564, 750, 815
DpnII GATC 6 cut(s) 46, 138, 535, 562, 748, 813
DrdI GACNNNNNNGTC 2 cut(s) 599, 628
DseDI GACNNNNNNGTC 2 cut(s) 599, 628
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 48, 746
EarI CTCTTC 2 cut(s) 48, 746
EciI GGCGGA 4 cut(s) 96, 225, 457, 707
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 650
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 5 cut(s) 4, 205, 373, 681, 800
Eco88I CYCGRG 1 cut(s) 641
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 717
FaeI CATG 2 cut(s) 619, 721
FaiI YATR 2 cut(s) 617, 719
FaqI GGGAC 2 cut(s) 191, 813
FatI CATG 2 cut(s) 615, 717
FauI CCCGC 1 cut(s) 433
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 650
FspBI CTAG 1 cut(s) 552
GsaI CCCAGC 1 cut(s) 520
HaeIII GGCC 4 cut(s) 273, 424, 489, 515
HapII CCGG 3 cut(s) 83, 509, 764
HgaI GACGC 1 cut(s) 589
Hin1II CATG 2 cut(s) 619, 721
HinfI GANTC 6 cut(s) 98, 248, 548, 575, 686, 734
HpaII CCGG 3 cut(s) 83, 509, 764
HphI GGTGA 3 cut(s) 133, 285, 385
Hpy166II GTNNAC 3 cut(s) 114, 373, 589
Hpy188I TCNGA 6 cut(s) 42, 97, 247, 406, 685, 813
Hpy188III TCNNGA 3 cut(s) 50, 539, 643
Hpy8I GTNNAC 3 cut(s) 114, 373, 589
Hpy99I CGWCG 3 cut(s) 596, 631, 634
HpyCH4III ACNGT 3 cut(s) 36, 92, 791
HpyCH4IV ACGT 1 cut(s) 626
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 179, 225, 512, 698
HpyF3I CTNAG 3 cut(s) 41, 673, 810
HpySE526I ACGT 1 cut(s) 626
Hsp92II CATG 2 cut(s) 619, 721
Kzo9I GATC 6 cut(s) 46, 138, 535, 562, 748, 813
LguI GCTCTTC 1 cut(s) 746
Lsp1109I GCAGC 4 cut(s) 33, 678, 681, 747
LweI GCATC 1 cut(s) 326
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 626
MalI GATC 6 cut(s) 48, 140, 537, 564, 750, 815
MboI GATC 6 cut(s) 46, 138, 535, 562, 748, 813
MboII GAAGA 3 cut(s) 7, 65, 733
MflI RGATCY 2 cut(s) 562, 813
MhlI GDGCHC 4 cut(s) 175, 642, 650, 773
MluCI AATT 2 cut(s) 118, 457
MlyI GAGTC 5 cut(s) 92, 542, 584, 680, 743
MmeI TCCRAC 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 792
MspI CCGG 3 cut(s) 83, 509, 764
MspR9I CCNGG 1 cut(s) 509
MvnI CGCG 3 cut(s) 369, 486, 560
MwoI GCNNNNNNNGC 5 cut(s) 27, 179, 225, 512, 698
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 717
NdeII GATC 6 cut(s) 46, 138, 535, 562, 748, 813
NlaIII CATG 2 cut(s) 619, 721
NlaIV GGNNCC 8 cut(s) 6, 207, 375, 490, 608, 682, 788, 801
NmeAIII GCCGAG 3 cut(s) 154, 668, 685
OliI CACNNNNGTG 1 cut(s) 792
PaeR7I CTCGAG 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 597
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 625
PflMI CCANNNNNTGG 1 cut(s) 616
PleI GAGTC 5 cut(s) 92, 542, 583, 680, 742
PpsI GAGTC 5 cut(s) 92, 542, 583, 680, 742
PspFI CCCAGC 1 cut(s) 516
PspN4I GGNNCC 8 cut(s) 6, 207, 375, 490, 608, 682, 788, 801
PspPI GGNCC 8 cut(s) 4, 205, 373, 423, 488, 514, 681, 800
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 2 cut(s) 562, 813
PsyI GACNNNGTC 1 cut(s) 625
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 746
Sau3AI GATC 6 cut(s) 46, 138, 535, 562, 748, 813
Sau96I GGNCC 8 cut(s) 4, 205, 373, 423, 488, 514, 681, 800
SchI GAGTC 5 cut(s) 92, 542, 584, 680, 743
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 4 cut(s) 175, 642, 650, 773
SfaNI GCATC 1 cut(s) 326
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 5 cut(s) 4, 205, 373, 681, 800
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 2 cut(s) 118, 457
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 3 cut(s) 36, 92, 791
TaiI ACGT 1 cut(s) 629
TaqI TCGA 2 cut(s) 632, 642
TaqII GACCGA 2 cut(s) 193, 515
TasI AATT 2 cut(s) 118, 457
TauI GCSGC 6 cut(s) 241, 369, 473, 476, 489, 704
TfiI GAWTC 1 cut(s) 248
TseI GCWGC 4 cut(s) 21, 666, 669, 760
Tth111I GACNNNGTC 1 cut(s) 625
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 5 cut(s) 4, 205, 373, 681, 800
XhoI CTCGAG 1 cut(s) 641
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.