RLG00000031844
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011269
Physical Location & Seq
Forward (+)
8684224 .. 8685472
1249 bp
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UTR
Exon/CDS
Intron
RLM00000031844

Sequence Viewer

Length: 825 bp
ATGGACTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGATTACAACAACACCACGAGCTCCACCCCGGAAAACAGCTCCGGCTCCCCTCCTCCCTCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCGTCTCCCAAGAAAAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAATCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCGCCAAACCCGGGATCAAAACCGAGACCCGGGAGGAGGAGACGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGCGGCGGTTCGCGGCGGATCTGGGCGGCGACGAGGAGTCGACGCTGGTGATCATCCCGAGCGCCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCCATTTTCGCTGAGAGCGGCGGCGGTGGGGCCGACACGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCTCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGCGTGGGAGGAGGAGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

275

Amino Acids

29.94

Weight (kDa)

5.25

Isoelectric Point (pI)

60.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 81 - 138 7.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
AatII GACGTC 2 cut(s) 126, 620
Acc36I ACCTGC 1 cut(s) 337
AccB1I GGYRCC 2 cut(s) 440, 774
AccBSI CCGCTC 2 cut(s) 517, 657
AccI GTMKAC 2 cut(s) 129, 554
AccII CGCG 5 cut(s) 363, 527, 602, 625, 783
AclWI GGATC 2 cut(s) 461, 540
AcoI YGGCCR 4 cut(s) 265, 416, 486, 490
AcyI GRCGYC 3 cut(s) 123, 441, 617
AfaI GTAC 2 cut(s) 177, 263
AfiI CCNNNNNNNGG 8 cut(s) 205, 238, 281, 418, 450, 468, 475, 784
AgsI TTSAA 2 cut(s) 22, 118
AhdI GACNNNNNGTC 1 cut(s) 550
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 2 cut(s) 251, 413
AleI CACNNNNGTG 1 cut(s) 380
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 4 cut(s) 75, 93, 321, 812
AluI AGCT 4 cut(s) 75, 93, 321, 812
Alw21I GWGCWC 3 cut(s) 77, 405, 761
Alw26I GTCTC 4 cut(s) 153, 458, 473, 729
AlwI GGATC 2 cut(s) 461, 540
Ama87I CYCGRG 4 cut(s) 449, 468, 571, 752
AoxI GGCC 6 cut(s) 249, 265, 416, 486, 490, 669
AspLEI GCGC 2 cut(s) 443, 578
AspS9I GGNCC 4 cut(s) 249, 367, 669, 803
AsuC2I CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
AsuHPI GGTGA 4 cut(s) 279, 379, 394, 574
AvaI CYCGRG 4 cut(s) 449, 468, 571, 752
AvaII GGWCC 2 cut(s) 367, 803
BanI GGYRCC 2 cut(s) 440, 774
BanII GRGCYC 3 cut(s) 77, 287, 641
BauI CACGAG 1 cut(s) 70
Bbv12I GWGCWC 3 cut(s) 77, 405, 761
BccI CCATC 3 cut(s) 284, 688, 700
BceAI ACGGC 5 cut(s) 220, 280, 454, 498, 563
BciT130I CCWGG 2 cut(s) 253, 415
BclI TGATCA 3 cut(s) 46, 384, 564
BcnI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
BcoDI GTCTC 4 cut(s) 153, 458, 473, 729
BfaI CTAG 1 cut(s) 426
BfoI RGCGCY 2 cut(s) 444, 579
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 438
BglII AGATCT 1 cut(s) 816
BisI GCNGC 8 cut(s) 361, 486, 518, 528, 541, 658, 661, 781
BlsI GCNGC 8 cut(s) 362, 487, 519, 529, 542, 659, 662, 782
Bme1390I CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
Bme18I GGWCC 2 cut(s) 367, 803
BmeRI GACNNNNNGTC 1 cut(s) 550
BmeT110I CYCGRG 4 cut(s) 449, 468, 571, 752
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 4 cut(s) 249, 367, 669, 803
BmiI GGNNCC 7 cut(s) 100, 369, 442, 501, 670, 776, 804
BmrFI CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
BpmI CTGGAG 2 cut(s) 274, 653
BpuMI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 3 cut(s) 123, 441, 617
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 6 cut(s) 81, 413, 436, 449, 468, 579
BsaXI ACNNNNNCTCC 6 cut(s) 211, 241, 506, 536, 783, 813
Bsc4I CCNNNNNNNGG 8 cut(s) 205, 238, 281, 418, 450, 468, 475, 784
Bse118I RCCGGY 3 cut(s) 165, 418, 488
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 253, 415
BseDI CCNNGG 6 cut(s) 81, 413, 436, 449, 468, 579
BseGI GGATG 3 cut(s) 567, 685, 699
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 8 cut(s) 205, 238, 281, 418, 450, 468, 475, 784
BseMII CTCAG 1 cut(s) 642
BseRI GAGGAG 7 cut(s) 96, 488, 491, 527, 563, 804, 807
BseX3I CGGCCG 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 5 cut(s) 363, 527, 602, 625, 783
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 6 cut(s) 251, 267, 418, 488, 492, 671
BshNI GGYRCC 2 cut(s) 440, 774
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 3 cut(s) 77, 405, 761
BsiHKCI CYCGRG 4 cut(s) 449, 468, 571, 752
BsiSI CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
BslFI GGGAC 1 cut(s) 816
BslI CCNNNNNNNGG 8 cut(s) 205, 238, 281, 418, 450, 468, 475, 784
BsmAI GTCTC 4 cut(s) 153, 458, 473, 729
BsmBI CGTCTC 2 cut(s) 153, 473
BsmFI GGGAC 1 cut(s) 816
BsnI GGCC 6 cut(s) 251, 267, 418, 488, 492, 671
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 4 cut(s) 449, 468, 571, 752
Bsp1286I GDGCHC 5 cut(s) 77, 287, 405, 641, 761
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
BspANI GGCC 6 cut(s) 251, 267, 418, 488, 492, 671
BspCNI CTCAG 1 cut(s) 643
BspFNI CGCG 5 cut(s) 363, 527, 602, 625, 783
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 7 cut(s) 100, 369, 442, 501, 670, 776, 804
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 461, 540
BspT107I GGYRCC 2 cut(s) 440, 774
BspTNI GGTCTC 1 cut(s) 458
BsrBI CCGCTC 2 cut(s) 517, 657
BsrFI RCCGGY 3 cut(s) 165, 418, 488
BsrGI TGTACA 1 cut(s) 175
BssAI RCCGGY 3 cut(s) 165, 418, 488
BssECI CCNNGG 6 cut(s) 81, 413, 436, 449, 468, 579
BssMI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
BssNI GRCGYC 3 cut(s) 123, 441, 617
BssSI CACGAG 1 cut(s) 70
Bst2BI CACGAG 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 253, 415
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 2 cut(s) 176, 708
BstACI GRCGYC 3 cut(s) 123, 441, 617
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 6 cut(s) 167, 171, 420, 490, 494, 761
BstDEI CTNAG 2 cut(s) 651, 813
BstDSI CCRYGG 2 cut(s) 436, 579
BstF5I GGATG 3 cut(s) 567, 685, 699
BstFNI CGCG 5 cut(s) 363, 527, 602, 625, 783
BstH2I RGCGCY 2 cut(s) 444, 579
BstHHI GCGC 2 cut(s) 443, 578
BstKTI GATC 8 cut(s) 49, 141, 387, 456, 535, 567, 739, 819
BstMAI GTCTC 4 cut(s) 153, 458, 473, 729
BstMBI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNI CCWGG 2 cut(s) 253, 415
BstSCI CCNGG 8 cut(s) 81, 251, 413, 448, 449, 467, 468, 507
BstUI CGCG 5 cut(s) 363, 527, 602, 625, 783
BstX2I RGATCY 2 cut(s) 532, 816
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 2 cut(s) 532, 816
BstZI CGGCCG 1 cut(s) 486
BsuRI GGCC 6 cut(s) 251, 267, 418, 488, 492, 671
BtgI CCRYGG 2 cut(s) 436, 579
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 3 cut(s) 567, 685, 699
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 6 cut(s) 167, 171, 420, 490, 494, 761
CciI TCATGA 1 cut(s) 33
CfoI GCGC 2 cut(s) 443, 578
Cfr10I RCCGGY 3 cut(s) 165, 418, 488
Cfr13I GGNCC 4 cut(s) 249, 367, 669, 803
Cfr9I CCCGGG 2 cut(s) 449, 468
CseI GACGC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 176, 262
CviAII CATG 3 cut(s) 34, 246, 607
CviQI GTAC 2 cut(s) 176, 262
DdeI CTNAG 2 cut(s) 651, 813
DinI GGCGCC 1 cut(s) 442
DpnI GATC 8 cut(s) 48, 140, 386, 455, 534, 566, 738, 818
DpnII GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
DriI GACNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 4 cut(s) 265, 416, 486, 490
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 2 cut(s) 176, 708
Eam1105I GACNNNNNGTC 1 cut(s) 550
EarI CTCTTC 2 cut(s) 176, 708
EciI GGCGGA 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 486
Eco24I GRGCYC 3 cut(s) 77, 287, 641
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 2 cut(s) 367, 803
Eco52I CGGCCG 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 75
Eco88I CYCGRG 4 cut(s) 449, 468, 571, 752
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 251, 413
EcoT38I GRGCYC 3 cut(s) 77, 287, 641
EgeI GGCGCC 1 cut(s) 442
EheI GGCGCC 1 cut(s) 442
Esp3I CGTCTC 2 cut(s) 153, 473
FaeI CATG 3 cut(s) 37, 249, 610
FaiI YATR 3 cut(s) 35, 247, 608
FaqI GGGAC 1 cut(s) 816
FatI CATG 3 cut(s) 33, 245, 606
FauI CCCGC 4 cut(s) 162, 240, 618, 682
FbaI TGATCA 3 cut(s) 46, 384, 564
FblI GTMKAC 2 cut(s) 129, 554
Fnu4HI GCNGC 8 cut(s) 361, 486, 518, 528, 541, 658, 661, 781
FokI GGATG 3 cut(s) 554, 692, 706
FriOI GRGCYC 3 cut(s) 77, 287, 641
FseI GGCCGGCC 1 cut(s) 492
Fsp4HI GCNGC 8 cut(s) 361, 486, 518, 528, 541, 658, 661, 781
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 442, 577
GluI GCNGC 8 cut(s) 361, 486, 518, 528, 541, 658, 661, 781
GsuI CTGGAG 2 cut(s) 274, 653
HaeII RGCGCY 2 cut(s) 444, 579
HaeIII GGCC 6 cut(s) 251, 267, 418, 488, 492, 671
HapII CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
HgaI GACGC 1 cut(s) 565
HhaI GCGC 2 cut(s) 443, 578
Hin1I GRCGYC 3 cut(s) 123, 441, 617
Hin1II CATG 3 cut(s) 37, 249, 610
Hin6I GCGC 2 cut(s) 441, 576
HinP1I GCGC 2 cut(s) 441, 576
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HinfI GANTC 4 cut(s) 5, 242, 551, 722
HpaII CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
HphI GGTGA 4 cut(s) 279, 379, 394, 574
Hpy166II GTNNAC 4 cut(s) 130, 367, 555, 583
Hpy188I TCNGA 3 cut(s) 241, 400, 816
Hpy188III TCNNGA 5 cut(s) 34, 50, 426, 571, 703
Hpy8I GTNNAC 4 cut(s) 130, 367, 555, 583
Hpy99I CGWCG 9 cut(s) 56, 125, 128, 241, 548, 559, 619, 622, 625
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 3 cut(s) 123, 617, 684
HpyCH4V TGCA 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 2 cut(s) 651, 813
HpySE526I ACGT 3 cut(s) 123, 617, 684
Hsp92I GRCGYC 3 cut(s) 123, 441, 617
Hsp92II CATG 3 cut(s) 37, 249, 610
HspAI GCGC 2 cut(s) 441, 576
KasI GGCGCC 1 cut(s) 440
KroI GCCGGC 3 cut(s) 165, 418, 488
KroNI GCCGGC 3 cut(s) 167, 420, 490
Ksp22I TGATCA 3 cut(s) 46, 384, 564
Kzo9I GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
LmnI GCTCC 4 cut(s) 80, 98, 104, 514
MaeI CTAG 1 cut(s) 426
MaeII ACGT 3 cut(s) 123, 617, 684
MaeIII GTNAC 1 cut(s) 215
MalI GATC 8 cut(s) 48, 140, 386, 455, 534, 566, 738, 818
MbiI CCGCTC 2 cut(s) 517, 657
MboI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
MboII GAAGA 3 cut(s) 10, 193, 725
MflI RGATCY 2 cut(s) 532, 816
MhlI GDGCHC 5 cut(s) 77, 287, 405, 641, 761
Mly113I GGCGCC 1 cut(s) 441
MlyI GAGTC 2 cut(s) 560, 731
MmeI TCCRAC 1 cut(s) 219
MroNI GCCGGC 3 cut(s) 165, 418, 488
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
MspR9I CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
MvaI CCWGG 2 cut(s) 253, 415
MvnI CGCG 5 cut(s) 363, 527, 602, 625, 783
MwoI GCNNNNNNNGC 1 cut(s) 438
NaeI GCCGGC 3 cut(s) 167, 420, 490
NarI GGCGCC 1 cut(s) 441
NciI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
NdeII GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
NgoMIV GCCGGC 3 cut(s) 165, 418, 488
NlaIII CATG 3 cut(s) 37, 249, 610
NlaIV GGNNCC 7 cut(s) 100, 369, 442, 501, 670, 776, 804
OliI CACNNNNGTG 1 cut(s) 380
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 337
PdiI GCCGGC 3 cut(s) 167, 420, 490
PfeI GAWTC 1 cut(s) 242
PflFI GACNNNGTC 2 cut(s) 125, 619
PkrI GCNGC 8 cut(s) 362, 487, 519, 529, 542, 659, 662, 782
PleI GAGTC 2 cut(s) 559, 730
PluTI GGCGCC 1 cut(s) 444
PpsI GAGTC 2 cut(s) 559, 730
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 251, 413
PspGI CCWGG 2 cut(s) 251, 413
PspN4I GGNNCC 7 cut(s) 100, 369, 442, 501, 670, 776, 804
PspPI GGNCC 4 cut(s) 249, 367, 669, 803
PsuI RGATCY 2 cut(s) 532, 816
PsyI GACNNNGTC 2 cut(s) 125, 619
RigI GGCCGGCC 1 cut(s) 492
RsaI GTAC 2 cut(s) 177, 263
RsaNI GTAC 2 cut(s) 176, 262
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 77
SalI GTCGAC 1 cut(s) 553
SatI GCNGC 8 cut(s) 361, 486, 518, 528, 541, 658, 661, 781
Sau3AI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 736, 816
Sau96I GGNCC 4 cut(s) 249, 367, 669, 803
SchI GAGTC 2 cut(s) 560, 731
ScrFI CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
SduI GDGCHC 5 cut(s) 77, 287, 405, 641, 761
SfoI GGCGCC 1 cut(s) 442
SinI GGWCC 2 cut(s) 367, 803
SmaI CCCGGG 2 cut(s) 451, 470
SmiMI CAYNNNNRTG 1 cut(s) 380
SspDI GGCGCC 1 cut(s) 440
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 8 cut(s) 81, 251, 413, 448, 449, 467, 468, 507
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 3 cut(s) 126, 620, 687
TaqI TCGA 1 cut(s) 554
TatI WGTACW 1 cut(s) 175
TauI GCSGC 8 cut(s) 363, 488, 520, 530, 543, 660, 663, 783
TfiI GAWTC 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 22, 234
TspGWI ACGGA 1 cut(s) 692
TspMI CCCGGG 2 cut(s) 449, 468
Tth111I GACNNNGTC 2 cut(s) 125, 619
VpaK11BI GGWCC 2 cut(s) 367, 803
XbaI TCTAGA 1 cut(s) 425
XmaI CCCGGG 2 cut(s) 449, 468
XmiI GTMKAC 2 cut(s) 129, 554
XspI CTAG 1 cut(s) 426
ZraI GACGTC 2 cut(s) 124, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.