Rh5BG084800
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011269
Physical Location & Seq
Forward (+)
7357084 .. 7358352
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG084800.1

Sequence Viewer

Length: 846 bp
ATGGACTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGATTACAACAACAACAACACCACCACGAGCTCCACCCCGGAAAACAGCTCCGGCTCCCCTCCTCCTCCTCCTCCCGCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCGTCTCCCAAGAAAAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAATCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCACGAAACCCGGAATCAAAACCGAGACCCGGGAGGAGGAGGCGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGAGGCGGTTCGCGGCGGATCTGGGCGGCGACGAGGAGTCGACGCTGGTGATCATCCCGAGCGCCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCCATTTTCGCTGAGAGCGGCGGCGGCGGTGGGGTCGACACGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCGCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGTGTGGGAGGAGGAGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

281

Amino Acids

30.63

Weight (kDa)

5.25

Isoelectric Point (pI)

61.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 87 - 144 7.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
AatII GACGTC 2 cut(s) 144, 638
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 795
AccBSI CCGCTC 1 cut(s) 675
AccI GTMKAC 3 cut(s) 147, 572, 693
AccII CGCG 4 cut(s) 381, 545, 620, 643
AclWI GGATC 1 cut(s) 558
AcoI YGGCCR 4 cut(s) 283, 434, 504, 508
AcyI GRCGYC 2 cut(s) 141, 635
AfaI GTAC 2 cut(s) 195, 281
AfiI CCNNNNNNNGG 7 cut(s) 223, 256, 299, 436, 486, 493, 805
AgsI TTSAA 2 cut(s) 22, 136
AhdI GACNNNNNGTC 1 cut(s) 568
AjiI CACGTC 1 cut(s) 706
AjnI CCWGG 2 cut(s) 269, 431
AleI CACNNNNGTG 2 cut(s) 398, 801
AloI GAACNNNNNNTCC 2 cut(s) 524, 556
AluBI AGCT 4 cut(s) 84, 102, 339, 833
AluI AGCT 4 cut(s) 84, 102, 339, 833
Alw21I GWGCWC 3 cut(s) 86, 423, 782
Alw26I GTCTC 2 cut(s) 171, 476
AlwI GGATC 1 cut(s) 558
Ama87I CYCGRG 3 cut(s) 486, 589, 773
AoxI GGCC 5 cut(s) 267, 283, 434, 504, 508
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 3 cut(s) 267, 385, 824
AsuC2I CCSGG 5 cut(s) 92, 468, 487, 488, 527
AsuHPI GGTGA 4 cut(s) 297, 397, 412, 592
AvaI CYCGRG 3 cut(s) 486, 589, 773
AvaII GGWCC 2 cut(s) 385, 824
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 3 cut(s) 86, 305, 659
BauI CACGAG 1 cut(s) 79
Bbv12I GWGCWC 3 cut(s) 86, 423, 782
BccI CCATC 3 cut(s) 302, 709, 721
BceAI ACGGC 5 cut(s) 238, 298, 472, 581, 786
BciT130I CCWGG 2 cut(s) 271, 433
BclI TGATCA 3 cut(s) 46, 402, 582
BcnI CCSGG 5 cut(s) 92, 468, 487, 488, 527
BcoDI GTCTC 2 cut(s) 171, 476
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 456
BglII AGATCT 1 cut(s) 837
BisI GCNGC 8 cut(s) 379, 501, 504, 546, 559, 676, 679, 682
BlsI GCNGC 8 cut(s) 380, 502, 505, 547, 560, 677, 680, 683
Bme1390I CCNGG 7 cut(s) 92, 271, 433, 468, 487, 488, 527
Bme18I GGWCC 2 cut(s) 385, 824
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 3 cut(s) 486, 589, 773
BmgBI CACGTC 1 cut(s) 706
BmgT120I GGNCC 3 cut(s) 267, 385, 824
BmiI GGNNCC 5 cut(s) 109, 387, 519, 797, 825
BmrFI CCNGG 7 cut(s) 92, 271, 433, 468, 487, 488, 527
BpmI CTGGAG 2 cut(s) 292, 671
BpuMI CCSGG 5 cut(s) 92, 468, 487, 488, 527
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 141, 635
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 5 cut(s) 90, 431, 454, 486, 597
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 524, 554, 804, 834
Bsc4I CCNNNNNNNGG 7 cut(s) 223, 256, 299, 436, 486, 493, 805
Bse118I RCCGGY 3 cut(s) 183, 436, 506
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 271, 433
BseDI CCNNGG 5 cut(s) 90, 431, 454, 486, 597
BseGI GGATG 3 cut(s) 585, 706, 720
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 7 cut(s) 223, 256, 299, 436, 486, 493, 805
BseMII CTCAG 1 cut(s) 660
BseX3I CGGCCG 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 352
Bsh1236I CGCG 4 cut(s) 381, 545, 620, 643
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 5 cut(s) 269, 285, 436, 506, 510
BshNI GGYRCC 1 cut(s) 795
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 86, 423, 782
BsiHKCI CYCGRG 3 cut(s) 486, 589, 773
BsiSI CCGG 9 cut(s) 92, 105, 184, 437, 468, 487, 507, 515, 527
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 7 cut(s) 223, 256, 299, 436, 486, 493, 805
BsmAI GTCTC 2 cut(s) 171, 476
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 837
BsnI GGCC 5 cut(s) 269, 285, 436, 506, 510
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 3 cut(s) 486, 589, 773
Bsp1286I GDGCHC 5 cut(s) 86, 305, 423, 659, 782
Bsp1407I TGTACA 1 cut(s) 193
Bsp143I GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
BspANI GGCC 5 cut(s) 269, 285, 436, 506, 510
BspCNI CTCAG 1 cut(s) 661
BspFNI CGCG 4 cut(s) 381, 545, 620, 643
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 5 cut(s) 109, 387, 519, 797, 825
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 795
BspTNI GGTCTC 1 cut(s) 476
BsrBI CCGCTC 1 cut(s) 675
BsrFI RCCGGY 3 cut(s) 183, 436, 506
BsrGI TGTACA 1 cut(s) 193
BssAI RCCGGY 3 cut(s) 183, 436, 506
BssECI CCNNGG 5 cut(s) 90, 431, 454, 486, 597
BssMI GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
BssNI GRCGYC 2 cut(s) 141, 635
BssSI CACGAG 1 cut(s) 79
Bst2BI CACGAG 1 cut(s) 79
Bst2UI CCWGG 2 cut(s) 271, 433
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 2 cut(s) 194, 729
BstACI GRCGYC 2 cut(s) 141, 635
BstAUI TGTACA 1 cut(s) 193
BstC8I GCNNGC 6 cut(s) 185, 189, 438, 508, 512, 782
BstDEI CTNAG 2 cut(s) 669, 834
BstDSI CCRYGG 2 cut(s) 454, 597
BstF5I GGATG 3 cut(s) 585, 706, 720
BstFNI CGCG 4 cut(s) 381, 545, 620, 643
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 7 cut(s) 49, 159, 405, 553, 585, 760, 840
BstMAI GTCTC 2 cut(s) 171, 476
BstMBI GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 2 cut(s) 456, 681
BstNI CCWGG 2 cut(s) 271, 433
BstSCI CCNGG 7 cut(s) 90, 269, 431, 466, 485, 486, 525
BstUI CGCG 4 cut(s) 381, 545, 620, 643
BstX2I RGATCY 2 cut(s) 550, 837
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 2 cut(s) 550, 837
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 5 cut(s) 269, 285, 436, 506, 510
BtgI CCRYGG 2 cut(s) 454, 597
BtrI CACGTC 1 cut(s) 706
BtsCI GGATG 3 cut(s) 585, 706, 720
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 6 cut(s) 185, 189, 438, 508, 512, 782
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 596
Cfr10I RCCGGY 3 cut(s) 183, 436, 506
Cfr13I GGNCC 3 cut(s) 267, 385, 824
Cfr9I CCCGGG 1 cut(s) 486
CseI GACGC 1 cut(s) 583
Csp6I GTAC 2 cut(s) 194, 280
CviAII CATG 3 cut(s) 34, 264, 625
CviQI GTAC 2 cut(s) 194, 280
DdeI CTNAG 2 cut(s) 669, 834
DpnI GATC 7 cut(s) 48, 158, 404, 552, 584, 759, 839
DpnII GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
DriI GACNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 4 cut(s) 283, 434, 504, 508
EagI CGGCCG 1 cut(s) 504
Eam1104I CTCTTC 2 cut(s) 194, 729
Eam1105I GACNNNNNGTC 1 cut(s) 568
EarI CTCTTC 2 cut(s) 194, 729
EciI GGCGGA 1 cut(s) 563
Ecl136II GAGCTC 1 cut(s) 84
EclXI CGGCCG 1 cut(s) 504
Eco24I GRGCYC 3 cut(s) 86, 305, 659
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 385, 824
Eco52I CGGCCG 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 84
Eco88I CYCGRG 3 cut(s) 486, 589, 773
EcoICRI GAGCTC 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 269, 431
EcoT38I GRGCYC 3 cut(s) 86, 305, 659
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 3 cut(s) 37, 267, 628
FaiI YATR 3 cut(s) 35, 265, 626
FaqI GGGAC 1 cut(s) 837
FatI CATG 3 cut(s) 33, 263, 624
FauI CCCGC 5 cut(s) 136, 180, 258, 636, 703
FbaI TGATCA 3 cut(s) 46, 402, 582
FblI GTMKAC 3 cut(s) 147, 572, 693
Fnu4HI GCNGC 8 cut(s) 379, 501, 504, 546, 559, 676, 679, 682
FokI GGATG 3 cut(s) 572, 713, 727
FriOI GRGCYC 3 cut(s) 86, 305, 659
FseI GGCCGGCC 1 cut(s) 510
Fsp4HI GCNGC 8 cut(s) 379, 501, 504, 546, 559, 676, 679, 682
FspBI CTAG 1 cut(s) 444
GlaI GCGC 1 cut(s) 595
GluI GCNGC 8 cut(s) 379, 501, 504, 546, 559, 676, 679, 682
GsuI CTGGAG 2 cut(s) 292, 671
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 5 cut(s) 269, 285, 436, 506, 510
HapII CCGG 9 cut(s) 92, 105, 184, 437, 468, 487, 507, 515, 527
HgaI GACGC 1 cut(s) 583
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 2 cut(s) 141, 635
Hin1II CATG 3 cut(s) 37, 267, 628
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 2 cut(s) 573, 694
HindII GTYRAC 2 cut(s) 573, 694
HinfI GANTC 5 cut(s) 5, 260, 471, 569, 743
HpaII CCGG 9 cut(s) 92, 105, 184, 437, 468, 487, 507, 515, 527
HphI GGTGA 4 cut(s) 297, 397, 412, 592
Hpy166II GTNNAC 5 cut(s) 148, 385, 573, 601, 694
Hpy188I TCNGA 3 cut(s) 259, 418, 837
Hpy188III TCNNGA 5 cut(s) 34, 50, 444, 589, 724
Hpy8I GTNNAC 5 cut(s) 148, 385, 573, 601, 694
Hpy99I CGWCG 9 cut(s) 56, 143, 146, 259, 566, 577, 637, 640, 643
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 3 cut(s) 141, 635, 705
HpyCH4V TGCA 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 2 cut(s) 456, 681
HpyF3I CTNAG 2 cut(s) 669, 834
HpySE526I ACGT 3 cut(s) 141, 635, 705
Hsp92I GRCGYC 2 cut(s) 141, 635
Hsp92II CATG 3 cut(s) 37, 267, 628
HspAI GCGC 1 cut(s) 594
KroI GCCGGC 3 cut(s) 183, 436, 506
KroNI GCCGGC 3 cut(s) 185, 438, 508
Ksp22I TGATCA 3 cut(s) 46, 402, 582
Kzo9I GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
LmnI GCTCC 3 cut(s) 89, 107, 113
MaeI CTAG 1 cut(s) 444
MaeII ACGT 3 cut(s) 141, 635, 705
MaeIII GTNAC 1 cut(s) 233
MalI GATC 7 cut(s) 48, 158, 404, 552, 584, 759, 839
MbiI CCGCTC 1 cut(s) 675
MboI GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
MboII GAAGA 3 cut(s) 10, 211, 746
MflI RGATCY 2 cut(s) 550, 837
MhlI GDGCHC 5 cut(s) 86, 305, 423, 659, 782
MlyI GAGTC 2 cut(s) 578, 752
MmeI TCCRAC 1 cut(s) 237
MroNI GCCGGC 3 cut(s) 183, 436, 506
MslI CAYNNNNRTG 2 cut(s) 398, 801
MspI CCGG 9 cut(s) 92, 105, 184, 437, 468, 487, 507, 515, 527
MspR9I CCNGG 7 cut(s) 92, 271, 433, 468, 487, 488, 527
MvaI CCWGG 2 cut(s) 271, 433
MvnI CGCG 4 cut(s) 381, 545, 620, 643
MwoI GCNNNNNNNGC 2 cut(s) 456, 681
NaeI GCCGGC 3 cut(s) 185, 438, 508
NciI CCSGG 5 cut(s) 92, 468, 487, 488, 527
NdeII GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
NgoMIV GCCGGC 3 cut(s) 183, 436, 506
NlaIII CATG 3 cut(s) 37, 267, 628
NlaIV GGNNCC 5 cut(s) 109, 387, 519, 797, 825
OliI CACNNNNGTG 2 cut(s) 398, 801
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 799
PdiI GCCGGC 3 cut(s) 185, 438, 508
PfeI GAWTC 2 cut(s) 260, 471
PflFI GACNNNGTC 2 cut(s) 143, 637
PkrI GCNGC 8 cut(s) 380, 502, 505, 547, 560, 677, 680, 683
PleI GAGTC 2 cut(s) 577, 751
PpsI GAGTC 2 cut(s) 577, 751
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 269, 431
PspGI CCWGG 2 cut(s) 269, 431
PspN4I GGNNCC 5 cut(s) 109, 387, 519, 797, 825
PspPI GGNCC 3 cut(s) 267, 385, 824
PsuI RGATCY 2 cut(s) 550, 837
PsyI GACNNNGTC 2 cut(s) 143, 637
RigI GGCCGGCC 1 cut(s) 510
RsaI GTAC 2 cut(s) 195, 281
RsaNI GTAC 2 cut(s) 194, 280
RseI CAYNNNNRTG 2 cut(s) 398, 801
SacI GAGCTC 1 cut(s) 86
SalI GTCGAC 2 cut(s) 571, 692
SatI GCNGC 8 cut(s) 379, 501, 504, 546, 559, 676, 679, 682
Sau3AI GATC 7 cut(s) 46, 156, 402, 550, 582, 757, 837
Sau96I GGNCC 3 cut(s) 267, 385, 824
SchI GAGTC 2 cut(s) 578, 752
ScrFI CCNGG 7 cut(s) 92, 271, 433, 468, 487, 488, 527
SduI GDGCHC 5 cut(s) 86, 305, 423, 659, 782
SinI GGWCC 2 cut(s) 385, 824
SmaI CCCGGG 1 cut(s) 488
SmiMI CAYNNNNRTG 2 cut(s) 398, 801
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 7 cut(s) 90, 269, 431, 466, 485, 486, 525
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 3 cut(s) 144, 638, 708
TaqI TCGA 2 cut(s) 572, 693
TatI WGTACW 1 cut(s) 193
TauI GCSGC 8 cut(s) 381, 503, 506, 548, 561, 678, 681, 684
TfiI GAWTC 2 cut(s) 260, 471
TspDTI ATGAA 2 cut(s) 22, 252
TspGWI ACGGA 1 cut(s) 713
TspMI CCCGGG 1 cut(s) 486
Tth111I GACNNNGTC 2 cut(s) 143, 637
VpaK11BI GGWCC 2 cut(s) 385, 824
XbaI TCTAGA 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 486
XmiI GTMKAC 3 cut(s) 147, 572, 693
XspI CTAG 1 cut(s) 444
ZraI GACGTC 2 cut(s) 142, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.