Rroxscaffold_1G00064910
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011269
Physical Location & Seq
Reverse (-)
86724015 .. 86725513
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064910.1

Sequence Viewer

Length: 843 bp
ATGGGCTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGATTACAACAACAACAACACCACGAGTTCCACCCCGGAAAACAGCTCCGGCTCCCCTCCTCCTCCTCCTCCTCCCGCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCGTCTCCCAAGAAAAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAATCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCACGAAACCCGGGATCAAAACCGAGACCCGGGAGGAGGAGGAGGAGACGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGAGGCGGTTCGCGCCGGATCTGGGCGGCGACGAGGAGTCGTCGCTGGTGATCATCCCGAGCCCCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCTATTTTCGCTGAGAGCGGCGGCGGCGGCGGTGGGGCCGACATGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCGCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGTGTGGTTGGAGGAGCTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

280

Amino Acids

30.47

Weight (kDa)

5.1

Isoelectric Point (pI)

68.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 87 - 140 5.9e-19 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
AatII GACGTC 2 cut(s) 144, 632
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 792
AccBSI CCGCTC 1 cut(s) 669
AccI GTMKAC 1 cut(s) 147
AccII CGCG 4 cut(s) 369, 539, 614, 637
AclWI GGATC 2 cut(s) 467, 552
AcoI YGGCCR 4 cut(s) 283, 422, 498, 502
AcyI GRCGYC 2 cut(s) 141, 629
AfaI GTAC 2 cut(s) 195, 281
AfiI CCNNNNNNNGG 8 cut(s) 223, 256, 299, 424, 474, 481, 548, 802
AgsI TTSAA 2 cut(s) 22, 136
AhdI GACNNNNNGTC 1 cut(s) 562
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 3 cut(s) 269, 314, 419
AleI CACNNNNGTG 2 cut(s) 386, 798
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 4 cut(s) 99, 327, 815, 830
AluI AGCT 4 cut(s) 99, 327, 815, 830
Alw21I GWGCWC 2 cut(s) 411, 779
Alw26I GTCTC 3 cut(s) 171, 464, 485
AlwI GGATC 2 cut(s) 467, 552
Ama87I CYCGRG 4 cut(s) 455, 474, 583, 770
AoxI GGCC 6 cut(s) 267, 283, 422, 498, 502, 687
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 4 cut(s) 267, 373, 687, 821
AsuC2I CCSGG 6 cut(s) 89, 456, 457, 475, 476, 521
AsuHPI GGTGA 4 cut(s) 297, 385, 400, 586
AvaI CYCGRG 4 cut(s) 455, 474, 583, 770
AvaII GGWCC 2 cut(s) 373, 821
BanI GGYRCC 1 cut(s) 792
BanII GRGCYC 4 cut(s) 8, 305, 590, 653
BauI CACGAG 1 cut(s) 76
Bbv12I GWGCWC 2 cut(s) 411, 779
BccI CCATC 3 cut(s) 302, 706, 718
BceAI ACGGC 5 cut(s) 238, 298, 460, 510, 783
BciT130I CCWGG 3 cut(s) 271, 316, 421
BclI TGATCA 3 cut(s) 46, 390, 576
BcnI CCSGG 6 cut(s) 89, 456, 457, 475, 476, 521
BcoDI GTCTC 3 cut(s) 171, 464, 485
BfaI CTAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 444
BglII AGATCT 1 cut(s) 834
BisI GCNGC 7 cut(s) 367, 498, 553, 670, 673, 676, 679
BlsI GCNGC 7 cut(s) 368, 499, 554, 671, 674, 677, 680
Bme1390I CCNGG 9 cut(s) 89, 271, 316, 421, 456, 457, 475, 476, 521
Bme18I GGWCC 2 cut(s) 373, 821
BmeRI GACNNNNNGTC 1 cut(s) 562
BmeT110I CYCGRG 4 cut(s) 455, 474, 583, 770
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 4 cut(s) 267, 373, 687, 821
BmiI GGNNCC 7 cut(s) 7, 106, 375, 513, 688, 794, 822
BmrFI CCNGG 9 cut(s) 89, 271, 316, 421, 456, 457, 475, 476, 521
BpmI CTGGAG 2 cut(s) 292, 665
BpuMI CCSGG 6 cut(s) 89, 456, 457, 475, 476, 521
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 141, 629
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 7 cut(s) 87, 314, 419, 442, 455, 474, 591
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 518, 548, 801, 831
Bsc4I CCNNNNNNNGG 8 cut(s) 223, 256, 299, 424, 474, 481, 548, 802
Bse118I RCCGGY 3 cut(s) 183, 424, 500
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 3 cut(s) 271, 316, 421
BseDI CCNNGG 7 cut(s) 87, 314, 419, 442, 455, 474, 591
BseGI GGATG 3 cut(s) 579, 703, 717
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 8 cut(s) 223, 256, 299, 424, 474, 481, 548, 802
BseMII CTCAG 1 cut(s) 654
BseX3I CGGCCG 1 cut(s) 498
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 4 cut(s) 369, 539, 614, 637
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 6 cut(s) 269, 285, 424, 500, 504, 689
BshNI GGYRCC 1 cut(s) 792
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 411, 779
BsiHKCI CYCGRG 4 cut(s) 455, 474, 583, 770
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 8 cut(s) 223, 256, 299, 424, 474, 481, 548, 802
BsmAI GTCTC 3 cut(s) 171, 464, 485
BsmBI CGTCTC 2 cut(s) 171, 485
BsmFI GGGAC 1 cut(s) 834
BsnI GGCC 6 cut(s) 269, 285, 424, 500, 504, 689
Bso31I GGTCTC 1 cut(s) 464
BsoBI CYCGRG 4 cut(s) 455, 474, 583, 770
Bsp1286I GDGCHC 6 cut(s) 8, 305, 411, 590, 653, 779
Bsp1407I TGTACA 1 cut(s) 193
Bsp143I GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
BspANI GGCC 6 cut(s) 269, 285, 424, 500, 504, 689
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 4 cut(s) 369, 539, 614, 637
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 7 cut(s) 7, 106, 375, 513, 688, 794, 822
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 2 cut(s) 467, 552
BspT107I GGYRCC 1 cut(s) 792
BspTNI GGTCTC 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 669
BsrFI RCCGGY 3 cut(s) 183, 424, 500
BsrGI TGTACA 1 cut(s) 193
BssAI RCCGGY 3 cut(s) 183, 424, 500
BssECI CCNNGG 7 cut(s) 87, 314, 419, 442, 455, 474, 591
BssMI GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
BssNI GRCGYC 2 cut(s) 141, 629
BssSI CACGAG 1 cut(s) 76
Bst2BI CACGAG 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 271, 316, 421
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 2 cut(s) 194, 726
BstACI GRCGYC 2 cut(s) 141, 629
BstAUI TGTACA 1 cut(s) 193
BstC8I GCNNGC 6 cut(s) 185, 189, 426, 502, 506, 779
BstDEI CTNAG 2 cut(s) 663, 831
BstDSI CCRYGG 2 cut(s) 442, 591
BstF5I GGATG 3 cut(s) 579, 703, 717
BstFNI CGCG 4 cut(s) 369, 539, 614, 637
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 8 cut(s) 49, 159, 393, 462, 547, 579, 757, 837
BstMAI GTCTC 3 cut(s) 171, 464, 485
BstMBI GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 4 cut(s) 444, 538, 675, 678
BstNI CCWGG 3 cut(s) 271, 316, 421
BstSCI CCNGG 9 cut(s) 87, 269, 314, 419, 454, 455, 473, 474, 519
BstUI CGCG 4 cut(s) 369, 539, 614, 637
BstX2I RGATCY 2 cut(s) 544, 834
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 544, 834
BstZI CGGCCG 1 cut(s) 498
BsuRI GGCC 6 cut(s) 269, 285, 424, 500, 504, 689
BtgI CCRYGG 2 cut(s) 442, 591
BtrI CACGTC 1 cut(s) 703
BtsCI GGATG 3 cut(s) 579, 703, 717
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 6 cut(s) 185, 189, 426, 502, 506, 779
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 541
Cfr10I RCCGGY 3 cut(s) 183, 424, 500
Cfr13I GGNCC 4 cut(s) 267, 373, 687, 821
Cfr9I CCCGGG 2 cut(s) 455, 474
Csp6I GTAC 2 cut(s) 194, 280
CviAII CATG 4 cut(s) 34, 264, 619, 694
CviQI GTAC 2 cut(s) 194, 280
DdeI CTNAG 2 cut(s) 663, 831
DpnI GATC 8 cut(s) 48, 158, 392, 461, 546, 578, 756, 836
DpnII GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
DriI GACNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 4 cut(s) 283, 422, 498, 502
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 2 cut(s) 194, 726
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 2 cut(s) 194, 726
EclXI CGGCCG 1 cut(s) 498
Eco24I GRGCYC 4 cut(s) 8, 305, 590, 653
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 373, 821
Eco52I CGGCCG 1 cut(s) 498
Eco88I CYCGRG 4 cut(s) 455, 474, 583, 770
EcoRII CCWGG 3 cut(s) 269, 314, 419
EcoT38I GRGCYC 4 cut(s) 8, 305, 590, 653
Esp3I CGTCTC 2 cut(s) 171, 485
FaeI CATG 4 cut(s) 37, 267, 622, 697
FaiI YATR 4 cut(s) 35, 265, 620, 695
FaqI GGGAC 1 cut(s) 834
FatI CATG 4 cut(s) 33, 263, 618, 693
FauI CCCGC 5 cut(s) 136, 180, 258, 630, 700
FbaI TGATCA 3 cut(s) 46, 390, 576
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 7 cut(s) 367, 498, 553, 670, 673, 676, 679
FokI GGATG 3 cut(s) 566, 710, 724
FriOI GRGCYC 4 cut(s) 8, 305, 590, 653
FseI GGCCGGCC 1 cut(s) 504
Fsp4HI GCNGC 7 cut(s) 367, 498, 553, 670, 673, 676, 679
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 540
GluI GCNGC 7 cut(s) 367, 498, 553, 670, 673, 676, 679
GsuI CTGGAG 2 cut(s) 292, 665
HaeIII GGCC 6 cut(s) 269, 285, 424, 500, 504, 689
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 2 cut(s) 141, 629
Hin1II CATG 4 cut(s) 37, 267, 622, 697
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HinfI GANTC 3 cut(s) 260, 563, 740
HphI GGTGA 4 cut(s) 297, 385, 400, 586
Hpy166II GTNNAC 3 cut(s) 148, 373, 595
Hpy188I TCNGA 3 cut(s) 259, 406, 834
Hpy188III TCNNGA 5 cut(s) 34, 50, 432, 583, 721
Hpy8I GTNNAC 3 cut(s) 148, 373, 595
Hpy99I CGWCG 9 cut(s) 56, 143, 146, 259, 560, 571, 631, 634, 637
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 3 cut(s) 141, 629, 702
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 4 cut(s) 444, 538, 675, 678
HpyF3I CTNAG 2 cut(s) 663, 831
HpySE526I ACGT 3 cut(s) 141, 629, 702
Hsp92I GRCGYC 2 cut(s) 141, 629
Hsp92II CATG 4 cut(s) 37, 267, 622, 697
HspAI GCGC 1 cut(s) 539
KroI GCCGGC 3 cut(s) 183, 424, 500
KroNI GCCGGC 3 cut(s) 185, 426, 502
Ksp22I TGATCA 3 cut(s) 46, 390, 576
Kzo9I GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
LmnI GCTCC 4 cut(s) 11, 104, 110, 812
MaeI CTAG 1 cut(s) 432
MaeII ACGT 3 cut(s) 141, 629, 702
MaeIII GTNAC 1 cut(s) 233
MalI GATC 8 cut(s) 48, 158, 392, 461, 546, 578, 756, 836
MbiI CCGCTC 1 cut(s) 669
MboI GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
MboII GAAGA 3 cut(s) 10, 211, 743
MflI RGATCY 2 cut(s) 544, 834
MhlI GDGCHC 6 cut(s) 8, 305, 411, 590, 653, 779
MlyI GAGTC 2 cut(s) 572, 749
MmeI TCCRAC 2 cut(s) 237, 787
MroNI GCCGGC 3 cut(s) 183, 424, 500
MslI CAYNNNNRTG 2 cut(s) 386, 798
MspR9I CCNGG 9 cut(s) 89, 271, 316, 421, 456, 457, 475, 476, 521
MvaI CCWGG 3 cut(s) 271, 316, 421
MvnI CGCG 4 cut(s) 369, 539, 614, 637
MwoI GCNNNNNNNGC 4 cut(s) 444, 538, 675, 678
NaeI GCCGGC 3 cut(s) 185, 426, 502
NciI CCSGG 6 cut(s) 89, 456, 457, 475, 476, 521
NdeII GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
NgoMIV GCCGGC 3 cut(s) 183, 424, 500
NlaIII CATG 4 cut(s) 37, 267, 622, 697
NlaIV GGNNCC 7 cut(s) 7, 106, 375, 513, 688, 794, 822
OliI CACNNNNGTG 2 cut(s) 386, 798
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 796
PdiI GCCGGC 3 cut(s) 185, 426, 502
PfeI GAWTC 1 cut(s) 260
PflFI GACNNNGTC 2 cut(s) 143, 631
PkrI GCNGC 7 cut(s) 368, 499, 554, 671, 674, 677, 680
PleI GAGTC 2 cut(s) 571, 748
PpsI GAGTC 2 cut(s) 571, 748
Psp6I CCWGG 3 cut(s) 269, 314, 419
PspGI CCWGG 3 cut(s) 269, 314, 419
PspN4I GGNNCC 7 cut(s) 7, 106, 375, 513, 688, 794, 822
PspPI GGNCC 4 cut(s) 267, 373, 687, 821
PsuI RGATCY 2 cut(s) 544, 834
PsyI GACNNNGTC 2 cut(s) 143, 631
RigI GGCCGGCC 1 cut(s) 504
RsaI GTAC 2 cut(s) 195, 281
RsaNI GTAC 2 cut(s) 194, 280
RseI CAYNNNNRTG 2 cut(s) 386, 798
SatI GCNGC 7 cut(s) 367, 498, 553, 670, 673, 676, 679
Sau3AI GATC 8 cut(s) 46, 156, 390, 459, 544, 576, 754, 834
Sau96I GGNCC 4 cut(s) 267, 373, 687, 821
SchI GAGTC 2 cut(s) 572, 749
ScrFI CCNGG 9 cut(s) 89, 271, 316, 421, 456, 457, 475, 476, 521
SduI GDGCHC 6 cut(s) 8, 305, 411, 590, 653, 779
SinI GGWCC 2 cut(s) 373, 821
SmaI CCCGGG 2 cut(s) 457, 476
SmiMI CAYNNNNRTG 2 cut(s) 386, 798
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 9 cut(s) 87, 269, 314, 419, 454, 455, 473, 474, 519
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 3 cut(s) 144, 632, 705
TatI WGTACW 1 cut(s) 193
TauI GCSGC 7 cut(s) 369, 500, 555, 672, 675, 678, 681
TfiI GAWTC 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 22, 252
TspMI CCCGGG 2 cut(s) 455, 474
Tth111I GACNNNGTC 2 cut(s) 143, 631
VpaK11BI GGWCC 2 cut(s) 373, 821
XbaI TCTAGA 1 cut(s) 431
XmaI CCCGGG 2 cut(s) 455, 474
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 1 cut(s) 432
ZraI GACGTC 2 cut(s) 142, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.