MD10G1275800.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011269
Physical Location & Seq
Reverse (-)
36698263 .. 36699996
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1275800.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGACCCCACATTCTTCAGCAGCAACAGCAAACAGATTATGAGCGACCAGGAGGAGAATGACAACACCACCTCCTCCACGCCGGAAAACAGCTCAGACTCTCCCCCGCCGTCCACCAATTTCAGCGACTACTCCAAGATCACCACTTCCACCTCCTCCCCCAAAAAAAGTGCTCGGCGAGCTATACAGAAACGAGTGGTGTCGGTACCCATAAAAAACGAGAACAGCAACACTCCGCCGCCGTCAGATTCTTGGGCTTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCCCCTTATCCCAGAGGATATTACAGGTGCAGTAGCTCAAAGGGATGCCCGGCGAGGAAACAAGTTGAAAGAAGCCGCGTGGACCCCACCATGCTGGTCATCACCTACTCCGCCGAACACAACCACCCTTGGCCCGCCTCTCGCAACCACAACAACAACCGCAACATTTCAAATCCCGCTGCCGGAACCAAACCCTGCCCCACCCCGGAGGCCCAGCAACCCGACCACCAAGACCAAGACCAAGACCCGAGGTTCGCGGATCTAAACAACGAGTCGCTTCTCGTAAACGACGAGTACGTCTGGTTCGCCGACATGGAGACGACGTCGTCGACGGTGCTCGAGAGCCCTATCTTCGCCGAGAGCGGCGGCTCCGATTCCGGGGACATGGCGGCAATGGTGTTCCCGATGGGAGAAGAGGACGAGTCTCTATTCGCCGATCTCGGCGAGCTGCCGGAGTATTCGCTCGTGTTTCGACACCGCGGCGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

267

Amino Acids

29.5

Weight (kDa)

5.1

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 83 - 140 3e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 587, 616
AatII GACGTC 1 cut(s) 617
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 620
AccII CGCG 3 cut(s) 369, 548, 771
AclWI GGATC 1 cut(s) 558
AcoI YGGCCR 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 3 cut(s) 207, 269, 587
AfiI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 669, 775
AgsI TTSAA 2 cut(s) 359, 462
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 5 cut(s) 93, 182, 327, 739, 788
AluI AGCT 5 cut(s) 93, 182, 327, 739, 788
Alw21I GWGCWC 2 cut(s) 175, 630
Alw26I GTCTC 2 cut(s) 602, 720
AlwI GGATC 1 cut(s) 558
Ama87I CYCGRG 2 cut(s) 538, 629
AoxI GGCC 3 cut(s) 271, 422, 501
ApeKI GCWGC 3 cut(s) 21, 470, 739
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp718I GGTACC 1 cut(s) 205
AspS9I GGNCC 5 cut(s) 4, 373, 423, 502, 779
AsuC2I CCSGG 3 cut(s) 341, 497, 670
AsuHPI GGTGA 2 cut(s) 133, 385
AvaI CYCGRG 2 cut(s) 538, 629
AvaII GGWCC 3 cut(s) 4, 373, 779
BanI GGYRCC 1 cut(s) 205
BanII GRGCYC 2 cut(s) 293, 638
BauI CACGAG 1 cut(s) 755
Bbv12I GWGCWC 2 cut(s) 175, 630
BbvI GCAGC 3 cut(s) 33, 457, 726
BccI CCATC 2 cut(s) 290, 691
BceAI ACGGC 3 cut(s) 94, 226, 286
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 3 cut(s) 341, 497, 670
BcoDI GTCTC 2 cut(s) 602, 720
BglII AGATCT 1 cut(s) 792
BisI GCNGC 9 cut(s) 22, 239, 367, 471, 655, 658, 681, 740, 772
BlsI GCNGC 9 cut(s) 23, 240, 368, 472, 656, 659, 682, 741, 773
Bme1390I CCNGG 4 cut(s) 50, 341, 497, 670
Bme18I GGWCC 3 cut(s) 4, 373, 779
BmeT110I CYCGRG 2 cut(s) 538, 629
BmgT120I GGNCC 5 cut(s) 4, 373, 423, 502, 779
BmiI GGNNCC 7 cut(s) 6, 207, 292, 375, 478, 661, 780
BmrFI CCNGG 4 cut(s) 50, 341, 497, 670
BmsI GCATC 1 cut(s) 326
BpuMI CCSGG 3 cut(s) 341, 497, 670
BsaHI GRCGYC 1 cut(s) 614
BsaJI CCNNGG 5 cut(s) 419, 495, 539, 669, 769
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 599, 629
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 473, 496, 669, 775
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 5 cut(s) 419, 495, 539, 669, 769
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 669, 775
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 3 cut(s) 64, 68, 145
BseXI GCAGC 3 cut(s) 33, 457, 726
BseYI CCCAGC 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 340
Bsh1236I CGCG 3 cut(s) 369, 548, 771
BshFI GGCC 3 cut(s) 273, 424, 503
BshNI GGYRCC 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 175, 630
BsiHKCI CYCGRG 2 cut(s) 538, 629
BsiSI CCGG 6 cut(s) 83, 341, 474, 497, 669, 743
BslFI GGGAC 2 cut(s) 686, 792
BslI CCNNNNNNNGG 5 cut(s) 287, 473, 496, 669, 775
BsmAI GTCTC 2 cut(s) 602, 720
BsmBI CGTCTC 1 cut(s) 602
BsmFI GGGAC 2 cut(s) 686, 792
BsnI GGCC 3 cut(s) 273, 424, 503
BsoBI CYCGRG 2 cut(s) 538, 629
Bsp1286I GDGCHC 4 cut(s) 175, 293, 630, 638
Bsp143I GATC 4 cut(s) 138, 550, 727, 792
BspANI GGCC 3 cut(s) 273, 424, 503
BspCNI CTCAG 1 cut(s) 107
BspFNI CGCG 3 cut(s) 369, 548, 771
BspLI GGNNCC 7 cut(s) 6, 207, 292, 375, 478, 661, 780
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 690
BssECI CCNNGG 5 cut(s) 419, 495, 539, 669, 769
BssMI GATC 4 cut(s) 138, 550, 727, 792
BssNI GRCGYC 1 cut(s) 614
BssSI CACGAG 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 1 cut(s) 699
BstACI GRCGYC 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 180, 426, 737
BstDEI CTNAG 2 cut(s) 94, 789
BstDSI CCRYGG 1 cut(s) 769
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 3 cut(s) 369, 548, 771
BstKTI GATC 4 cut(s) 141, 553, 730, 795
BstMAI GTCTC 2 cut(s) 602, 720
BstMBI GATC 4 cut(s) 138, 550, 727, 792
BstMWI GCNNNNNNNGC 2 cut(s) 27, 179
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 4 cut(s) 48, 339, 495, 668
BstUI CGCG 3 cut(s) 369, 548, 771
BstV1I GCAGC 3 cut(s) 33, 457, 726
BstX2I RGATCY 2 cut(s) 550, 792
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 550, 792
BsuRI GGCC 3 cut(s) 273, 424, 503
BtgI CCRYGG 1 cut(s) 769
BtsCI GGATG 1 cut(s) 341
Cac8I GCNNGC 3 cut(s) 180, 426, 737
Cfr13I GGNCC 5 cut(s) 4, 373, 423, 502, 779
Cfr42I CCGCGG 1 cut(s) 772
Csp6I GTAC 3 cut(s) 206, 268, 586
CviAII CATG 3 cut(s) 382, 604, 676
CviQI GTAC 3 cut(s) 206, 268, 586
DdeI CTNAG 2 cut(s) 94, 789
DpnI GATC 4 cut(s) 140, 552, 729, 794
DpnII GATC 4 cut(s) 138, 550, 727, 792
DrdI GACNNNNNNGTC 2 cut(s) 587, 616
DseDI GACNNNNNNGTC 2 cut(s) 587, 616
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
EciI GGCGGA 2 cut(s) 225, 391
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 2 cut(s) 293, 638
Eco47I GGWCC 3 cut(s) 4, 373, 779
Eco88I CYCGRG 2 cut(s) 538, 629
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 2 cut(s) 293, 638
ErhI CCWWGG 1 cut(s) 419
Esp3I CGTCTC 1 cut(s) 602
FaeI CATG 3 cut(s) 385, 607, 679
FaiI YATR 6 cut(s) 41, 185, 212, 383, 605, 677
FaqI GGGAC 2 cut(s) 686, 792
FatI CATG 3 cut(s) 381, 603, 675
FauI CCCGC 3 cut(s) 114, 433, 475
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 9 cut(s) 22, 239, 367, 471, 655, 658, 681, 740, 772
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 2 cut(s) 293, 638
Fsp4HI GCNGC 9 cut(s) 22, 239, 367, 471, 655, 658, 681, 740, 772
GluI GCNGC 9 cut(s) 22, 239, 367, 471, 655, 658, 681, 740, 772
GsaI CCCAGC 1 cut(s) 508
HaeIII GGCC 3 cut(s) 273, 424, 503
HapII CCGG 6 cut(s) 83, 341, 474, 497, 669, 743
Hin1I GRCGYC 1 cut(s) 614
Hin1II CATG 3 cut(s) 385, 607, 679
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 5 cut(s) 98, 248, 563, 665, 713
HpaII CCGG 6 cut(s) 83, 341, 474, 497, 669, 743
HphI GGTGA 2 cut(s) 133, 385
Hpy166II GTNNAC 4 cut(s) 114, 373, 577, 621
Hpy188I TCNGA 4 cut(s) 97, 247, 664, 792
Hpy188III TCNNGA 2 cut(s) 631, 694
Hpy8I GTNNAC 4 cut(s) 114, 373, 577, 621
Hpy99I CGWCG 5 cut(s) 584, 616, 619, 622, 625
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 2 cut(s) 588, 614
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 179
HpyF3I CTNAG 2 cut(s) 94, 789
HpySE526I ACGT 2 cut(s) 588, 614
Hsp92I GRCGYC 1 cut(s) 614
Hsp92II CATG 3 cut(s) 385, 607, 679
KpnI GGTACC 1 cut(s) 209
KspI CCGCGG 1 cut(s) 772
Kzo9I GATC 4 cut(s) 138, 550, 727, 792
LmnI GCTCC 2 cut(s) 296, 665
Lsp1109I GCAGC 3 cut(s) 33, 457, 726
LweI GCATC 1 cut(s) 326
MaeII ACGT 2 cut(s) 588, 614
MalI GATC 4 cut(s) 140, 552, 729, 794
MbiI CCGCTC 1 cut(s) 654
MboI GATC 4 cut(s) 138, 550, 727, 792
MboII GAAGA 3 cut(s) 7, 634, 716
MflI RGATCY 2 cut(s) 550, 792
MhlI GDGCHC 4 cut(s) 175, 293, 630, 638
MluCI AATT 1 cut(s) 118
MlyI GAGTC 3 cut(s) 92, 572, 722
MspA1I CMGCKG 2 cut(s) 470, 771
MspI CCGG 6 cut(s) 83, 341, 474, 497, 669, 743
MspR9I CCNGG 4 cut(s) 50, 341, 497, 670
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 3 cut(s) 369, 548, 771
MwoI GCNNNNNNNGC 2 cut(s) 27, 179
NciI CCSGG 3 cut(s) 341, 497, 670
NdeII GATC 4 cut(s) 138, 550, 727, 792
NlaIII CATG 3 cut(s) 385, 607, 679
NlaIV GGNNCC 7 cut(s) 6, 207, 292, 375, 478, 661, 780
NmeAIII GCCGAG 3 cut(s) 154, 673, 711
PaeR7I CTCGAG 1 cut(s) 629
PcsI WCGNNNNNNNCGW 3 cut(s) 579, 585, 617
PfeI GAWTC 2 cut(s) 248, 665
PflFI GACNNNGTC 2 cut(s) 613, 616
PkrI GCNGC 9 cut(s) 23, 240, 368, 472, 656, 659, 682, 741, 773
PleI GAGTC 3 cut(s) 92, 571, 721
PpsI GAGTC 3 cut(s) 92, 571, 721
Psp6I CCWGG 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 504
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 7 cut(s) 6, 207, 292, 375, 478, 661, 780
PspPI GGNCC 5 cut(s) 4, 373, 423, 502, 779
PsrI GAACNNNNNNTAC 2 cut(s) 578, 610
PsuI RGATCY 2 cut(s) 550, 792
PsyI GACNNNGTC 2 cut(s) 613, 616
RsaI GTAC 3 cut(s) 207, 269, 587
RsaNI GTAC 3 cut(s) 206, 268, 586
SacII CCGCGG 1 cut(s) 772
SalI GTCGAC 1 cut(s) 619
SatI GCNGC 9 cut(s) 22, 239, 367, 471, 655, 658, 681, 740, 772
Sau3AI GATC 4 cut(s) 138, 550, 727, 792
Sau96I GGNCC 5 cut(s) 4, 373, 423, 502, 779
SchI GAGTC 3 cut(s) 92, 572, 722
ScrFI CCNGG 4 cut(s) 50, 341, 497, 670
SduI GDGCHC 4 cut(s) 175, 293, 630, 638
SfaNI GCATC 1 cut(s) 326
Sfr274I CTCGAG 1 cut(s) 629
Sfr303I CCGCGG 1 cut(s) 772
SgrBI CCGCGG 1 cut(s) 772
SgrDI CGTCGACG 1 cut(s) 619
SinI GGWCC 3 cut(s) 4, 373, 779
SlaI CTCGAG 1 cut(s) 629
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 1 cut(s) 118
StyD4I CCNGG 4 cut(s) 48, 339, 495, 668
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 2 cut(s) 591, 617
TaqI TCGA 3 cut(s) 620, 630, 763
TasI AATT 1 cut(s) 118
TauI GCSGC 6 cut(s) 241, 369, 657, 660, 683, 774
TfiI GAWTC 2 cut(s) 248, 665
TseI GCWGC 3 cut(s) 21, 470, 739
Tth111I GACNNNGTC 2 cut(s) 613, 616
VpaK11BI GGWCC 3 cut(s) 4, 373, 779
XhoI CTCGAG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 620
ZraI GACGTC 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.