Prupe.4G066400_v2.0.a1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011269
Physical Location & Seq
Forward (+)
3205798 .. 3207551
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G066400.1

Sequence Viewer

Length: 852 bp
ATGGACTCTACATTCTTCAACAGCAACAACAGCAACAGCAGAAGCAAGCAGTTTATGAGCGATCAAGAAGAGAACGACAATACTACCAGCTCCACACCAGAAAATAGCTCAGGCTCTCCTCCTCCACCCACTACCAATTTCAGTGACTTCTCCAAGATCACTTCCACGTCCTCCCCCAAGAAAAGTGCTCGGCGAGCCATACAGAAACGAGTGGTGTCGATACCCATCAAAGGCGACAACAGCAACACTCCGCCTCCGTCCGATTCTTGGGCTTGGAGAAAGTACGGCCAAAAACCCATCAAGGGCTCGCCTTATCCCAGAGGATATTACAGGTGCAGTAGCTCAAAGGGCTGCCCGGCAAGGAAGCAAGTAGAGAGAAGCCGCGTGGACCCCTCCATGCTCGTCATCACCTACTCCTCCGAACACAACCACCCCTGGCCCGCTTCTCGCAACCACCACAACCATCACCAGAGCAACAGCTCGAGCTCTGCCGCCGCCTCCGCCGCCGTCACAAACAAACCCGGCTCCAACAAGACCGAAGCCCCGGAAGCCCAGCAACCCGACCACCAAGACCACGACCCGACATTCGCGGATCTCAACGACGAGTCGCTCCTCACACACGACGAGTTCGGCTGGTTTGCCGACATGGAAACGACGTCGTCGACGGTGCTCGAGAGCCCAATTTTCACGGAGAGCGGCTGCGCTGGTGGGGCCGACTCCGCTGACATGGCAGCGATGGTTTTCCCGATGGGGGAAGAGGACGAGTCGCTCTTCGCTGATCTGGGCGAGCTGCCGGAGTGCTCTCTCGTTTTTCGGCACCGGGGGGTGGGACCACAAGTTCAGATCTGTTGA

Protein Analysis

284

Amino Acids

30.92

Weight (kDa)

5.56

Isoelectric Point (pI)

56.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 816
AccBSI CCGCTC 1 cut(s) 696
AccI GTMKAC 1 cut(s) 662
AccII CGCG 2 cut(s) 384, 590
AclWI GGATC 1 cut(s) 600
AcoI YGGCCR 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 5 cut(s) 230, 267, 302, 751, 826
AgsI TTSAA 1 cut(s) 19
AjiI CACGTC 1 cut(s) 168
AjnI CCWGG 1 cut(s) 434
AloI GAACNNNNNNTCC 1 cut(s) 822
AluBI AGCT 6 cut(s) 90, 108, 342, 480, 486, 790
AluI AGCT 6 cut(s) 90, 108, 342, 480, 486, 790
Alw21I GWGCWC 4 cut(s) 190, 488, 672, 803
AlwI GGATC 1 cut(s) 600
Ama87I CYCGRG 2 cut(s) 481, 671
AoxI GGCC 3 cut(s) 286, 437, 711
ApeKI GCWGC 4 cut(s) 351, 699, 731, 790
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 4 cut(s) 388, 438, 711, 830
AsuC2I CCSGG 4 cut(s) 356, 522, 545, 821
AsuHPI GGTGA 2 cut(s) 400, 458
AvaI CYCGRG 2 cut(s) 481, 671
AvaII GGWCC 2 cut(s) 388, 830
BanI GGYRCC 1 cut(s) 816
BanII GRGCYC 3 cut(s) 308, 488, 680
Bbv12I GWGCWC 4 cut(s) 190, 488, 672, 803
BbvI GCAGC 4 cut(s) 338, 686, 743, 777
BccI CCATC 5 cut(s) 233, 305, 471, 730, 742
BceAI ACGGC 2 cut(s) 301, 491
BciT130I CCWGG 1 cut(s) 436
BcnI CCSGG 4 cut(s) 356, 522, 545, 821
BglII AGATCT 1 cut(s) 843
BisI GCNGC 9 cut(s) 352, 382, 492, 495, 504, 697, 700, 732, 791
BlsI GCNGC 9 cut(s) 353, 383, 493, 496, 505, 698, 701, 733, 792
Bme1390I CCNGG 5 cut(s) 356, 436, 522, 545, 821
Bme18I GGWCC 2 cut(s) 388, 830
BmeT110I CYCGRG 2 cut(s) 481, 671
BmgBI CACGTC 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 388, 438, 711, 830
BmiI GGNNCC 5 cut(s) 390, 526, 712, 818, 831
BmrFI CCNGG 5 cut(s) 356, 436, 522, 545, 821
Bpu10I CCTNAGC 1 cut(s) 109
BpuMI CCSGG 4 cut(s) 356, 522, 545, 821
BsaBI GATNNNNATC 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 3 cut(s) 434, 543, 820
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 267, 302, 751, 826
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 3 cut(s) 434, 543, 820
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 5 cut(s) 230, 267, 302, 751, 826
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 4 cut(s) 108, 111, 406, 602
BseXI GCAGC 4 cut(s) 338, 686, 743, 777
BseYI CCCAGC 1 cut(s) 552
BsgI GTGCAG 1 cut(s) 355
Bsh1236I CGCG 2 cut(s) 384, 590
BshFI GGCC 3 cut(s) 288, 439, 713
BshNI GGYRCC 1 cut(s) 816
BsiHKAI GWGCWC 4 cut(s) 190, 488, 672, 803
BsiHKCI CYCGRG 2 cut(s) 481, 671
BsiSI CCGG 5 cut(s) 356, 522, 545, 794, 820
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 5 cut(s) 230, 267, 302, 751, 826
BsmFI GGGAC 1 cut(s) 843
BsnI GGCC 3 cut(s) 288, 439, 713
BsoBI CYCGRG 2 cut(s) 481, 671
Bsp1286I GDGCHC 6 cut(s) 190, 308, 488, 672, 680, 803
Bsp143I GATC 5 cut(s) 61, 156, 592, 778, 843
BspANI GGCC 3 cut(s) 288, 439, 713
BspCNI CTCAG 1 cut(s) 122
BspFNI CGCG 2 cut(s) 384, 590
BspLI GGNNCC 5 cut(s) 390, 526, 712, 818, 831
BspPI GGATC 1 cut(s) 600
BspQI GCTCTTC 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 816
BsrBI CCGCTC 1 cut(s) 696
BssECI CCNNGG 3 cut(s) 434, 543, 820
BssMI GATC 5 cut(s) 61, 156, 592, 778, 843
BssNI GRCGYC 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 1 cut(s) 667
Bst6I CTCTTC 3 cut(s) 63, 750, 776
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 5 cut(s) 47, 195, 308, 441, 788
BstDEI CTNAG 1 cut(s) 109
BstFNI CGCG 2 cut(s) 384, 590
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 5 cut(s) 64, 159, 595, 781, 846
BstMBI GATC 5 cut(s) 61, 156, 592, 778, 843
BstNI CCWGG 1 cut(s) 436
BstSCI CCNGG 5 cut(s) 354, 434, 520, 543, 819
BstUI CGCG 2 cut(s) 384, 590
BstV1I GCAGC 4 cut(s) 338, 686, 743, 777
BstX2I RGATCY 2 cut(s) 592, 843
BstYI RGATCY 2 cut(s) 592, 843
BsuRI GGCC 3 cut(s) 288, 439, 713
BtgZI GCGATG 1 cut(s) 749
BtrI CACGTC 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 5 cut(s) 47, 195, 308, 441, 788
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 4 cut(s) 388, 438, 711, 830
Csp6I GTAC 1 cut(s) 283
CviAII CATG 3 cut(s) 397, 646, 727
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 109
DpnI GATC 5 cut(s) 63, 158, 594, 780, 845
DpnII GATC 5 cut(s) 61, 156, 592, 778, 843
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 63, 750, 776
EarI CTCTTC 3 cut(s) 63, 750, 776
EciI GGCGGA 2 cut(s) 240, 490
Ecl136II GAGCTC 1 cut(s) 486
Eco24I GRGCYC 3 cut(s) 308, 488, 680
Eco47I GGWCC 2 cut(s) 388, 830
Eco53kI GAGCTC 1 cut(s) 486
Eco88I CYCGRG 2 cut(s) 481, 671
EcoICRI GAGCTC 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 434
EcoT38I GRGCYC 3 cut(s) 308, 488, 680
FaeI CATG 3 cut(s) 400, 649, 730
FaiI YATR 5 cut(s) 56, 200, 398, 647, 728
FaqI GGGAC 1 cut(s) 843
FatI CATG 3 cut(s) 396, 645, 726
FauI CCCGC 1 cut(s) 448
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 9 cut(s) 352, 382, 492, 495, 504, 697, 700, 732, 791
FriOI GRGCYC 3 cut(s) 308, 488, 680
Fsp4HI GCNGC 9 cut(s) 352, 382, 492, 495, 504, 697, 700, 732, 791
GlaI GCGC 1 cut(s) 703
GluI GCNGC 9 cut(s) 352, 382, 492, 495, 504, 697, 700, 732, 791
GsaI CCCAGC 1 cut(s) 556
HaeIII GGCC 3 cut(s) 288, 439, 713
HapII CCGG 5 cut(s) 356, 522, 545, 794, 820
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 3 cut(s) 400, 649, 730
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 5 cut(s) 5, 263, 605, 716, 764
HpaII CCGG 5 cut(s) 356, 522, 545, 794, 820
HphI GGTGA 2 cut(s) 400, 458
Hpy166II GTNNAC 2 cut(s) 388, 663
Hpy188I TCNGA 3 cut(s) 262, 421, 843
Hpy188III TCNNGA 3 cut(s) 65, 673, 745
Hpy8I GTNNAC 2 cut(s) 388, 663
Hpy99I CGWCG 6 cut(s) 605, 626, 658, 661, 664, 667
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 2 cut(s) 167, 656
HpyCH4V TGCA 1 cut(s) 336
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 2 cut(s) 167, 656
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 3 cut(s) 400, 649, 730
HspAI GCGC 1 cut(s) 702
Kzo9I GATC 5 cut(s) 61, 156, 592, 778, 843
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 3 cut(s) 95, 530, 615
Lsp1109I GCAGC 4 cut(s) 338, 686, 743, 777
MaeII ACGT 2 cut(s) 167, 656
MaeIII GTNAC 2 cut(s) 143, 508
MalI GATC 5 cut(s) 63, 158, 594, 780, 845
MbiI CCGCTC 1 cut(s) 696
MboI GATC 5 cut(s) 61, 156, 592, 778, 843
MboII GAAGA 4 cut(s) 7, 80, 763, 767
MflI RGATCY 2 cut(s) 592, 843
MhlI GDGCHC 6 cut(s) 190, 308, 488, 672, 680, 803
MluCI AATT 2 cut(s) 136, 681
MlyI GAGTC 3 cut(s) 614, 710, 773
MmeI TCCRAC 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 5 cut(s) 356, 522, 545, 794, 820
MspR9I CCNGG 5 cut(s) 356, 436, 522, 545, 821
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 384, 590
NciI CCSGG 4 cut(s) 356, 522, 545, 821
NdeII GATC 5 cut(s) 61, 156, 592, 778, 843
NlaIII CATG 3 cut(s) 400, 649, 730
NlaIV GGNNCC 5 cut(s) 390, 526, 712, 818, 831
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 143, 508
PaeR7I CTCGAG 2 cut(s) 481, 671
PciSI GCTCTTC 1 cut(s) 776
PcsI WCGNNNNNNNCGW 1 cut(s) 659
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 658
PkrI GCNGC 9 cut(s) 353, 383, 493, 496, 505, 698, 701, 733, 792
PleI GAGTC 3 cut(s) 613, 710, 772
PpsI GAGTC 3 cut(s) 613, 710, 772
Psp124BI GAGCTC 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 5 cut(s) 390, 526, 712, 818, 831
PspPI GGNCC 4 cut(s) 388, 438, 711, 830
PspXI VCTCGAGB 1 cut(s) 481
PsuI RGATCY 2 cut(s) 592, 843
PsyI GACNNNGTC 1 cut(s) 658
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 1 cut(s) 488
SalI GTCGAC 1 cut(s) 661
SapI GCTCTTC 1 cut(s) 776
SatI GCNGC 9 cut(s) 352, 382, 492, 495, 504, 697, 700, 732, 791
Sau3AI GATC 5 cut(s) 61, 156, 592, 778, 843
Sau96I GGNCC 4 cut(s) 388, 438, 711, 830
SchI GAGTC 3 cut(s) 614, 710, 773
ScrFI CCNGG 5 cut(s) 356, 436, 522, 545, 821
SduI GDGCHC 6 cut(s) 190, 308, 488, 672, 680, 803
Sfr274I CTCGAG 2 cut(s) 481, 671
SgrDI CGTCGACG 1 cut(s) 661
SinI GGWCC 2 cut(s) 388, 830
SlaI CTCGAG 2 cut(s) 481, 671
SmlI CTYRAG 2 cut(s) 481, 671
SmoI CTYRAG 2 cut(s) 481, 671
Sse9I AATT 2 cut(s) 136, 681
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 5 cut(s) 354, 434, 520, 543, 819
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 2 cut(s) 170, 659
TaqI TCGA 4 cut(s) 218, 482, 662, 672
TaqII GACCGA 1 cut(s) 551
TasI AATT 2 cut(s) 136, 681
TauI GCSGC 5 cut(s) 384, 494, 497, 506, 699
TfiI GAWTC 1 cut(s) 263
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 2 cut(s) 143, 508
TseI GCWGC 4 cut(s) 351, 699, 731, 790
Tsp45I GTSAC 2 cut(s) 143, 508
TspGWI ACGGA 2 cut(s) 246, 704
TspRI CASTG 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 658
VpaK11BI GGWCC 2 cut(s) 388, 830
XhoI CTCGAG 2 cut(s) 481, 671
XmiI GTMKAC 1 cut(s) 662
ZraI GACGTC 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.