RchiOBHm_Chr5g0011581
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011269
Physical Location & Seq
Forward (+)
7677612 .. 7679242
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29222

Sequence Viewer

Length: 834 bp
ATGGGCTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGATTACAACAACAACACGAGCTCCACCCCGGAAAACAGCTCCGGCTCCCCTCCTCCTCCTCCTCCTCCCGCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCGTCTCCCAAGAAAAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAGTCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCACGGCACGAAACCCGGGATCAAAGCCGAGACCCGGGAGGAGGAGACGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGAGGCGGTTCGCGGCGGATCTGGGCGGCGACGAGGAGTCGTCGCTGGTGATCATCCCGAGCGTCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCCATTTTCGCTGAGAGCGGCGGTGGGGCCGACACGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCGCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGTGTGGGAGGAGGAGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

277

Amino Acids

30.21

Weight (kDa)

5.34

Isoelectric Point (pI)

62.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 85 - 142 7.4e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 349
AatII GACGTC 2 cut(s) 138, 632
Acc36I ACCTGC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 783
AccBSI CCGCTC 1 cut(s) 669
AccI GTMKAC 1 cut(s) 141
AccII CGCG 4 cut(s) 375, 539, 614, 637
AclWI GGATC 2 cut(s) 473, 552
AcoI YGGCCR 4 cut(s) 277, 428, 498, 502
AcyI GRCGYC 2 cut(s) 135, 629
AfaI GTAC 2 cut(s) 189, 275
AfiI CCNNNNNNNGG 7 cut(s) 217, 250, 293, 430, 480, 487, 793
AgsI TTSAA 2 cut(s) 22, 130
AhdI GACNNNNNGTC 1 cut(s) 562
AjiI CACGTC 1 cut(s) 694
AjnI CCWGG 2 cut(s) 263, 425
AleI CACNNNNGTG 2 cut(s) 392, 789
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 4 cut(s) 75, 93, 333, 821
AluI AGCT 4 cut(s) 75, 93, 333, 821
Alw21I GWGCWC 3 cut(s) 77, 417, 770
Alw26I GTCTC 3 cut(s) 165, 470, 485
AlwI GGATC 2 cut(s) 473, 552
Ama87I CYCGRG 4 cut(s) 461, 480, 583, 761
AoxI GGCC 6 cut(s) 261, 277, 428, 498, 502, 678
AspS9I GGNCC 4 cut(s) 261, 379, 678, 812
AsuC2I CCSGG 6 cut(s) 83, 462, 463, 481, 482, 521
AsuHPI GGTGA 4 cut(s) 291, 391, 406, 586
AvaI CYCGRG 4 cut(s) 461, 480, 583, 761
AvaII GGWCC 2 cut(s) 379, 812
BanI GGYRCC 1 cut(s) 783
BanII GRGCYC 4 cut(s) 8, 77, 299, 653
BauI CACGAG 1 cut(s) 70
Bbv12I GWGCWC 3 cut(s) 77, 417, 770
BccI CCATC 3 cut(s) 296, 697, 709
BceAI ACGGC 5 cut(s) 232, 292, 466, 510, 774
BciT130I CCWGG 2 cut(s) 265, 427
BclI TGATCA 3 cut(s) 46, 396, 576
BcnI CCSGG 6 cut(s) 83, 462, 463, 481, 482, 521
BcoDI GTCTC 3 cut(s) 165, 470, 485
BfaI CTAG 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 349
BglI GCCNNNNNGGC 1 cut(s) 450
BglII AGATCT 1 cut(s) 825
BisI GCNGC 5 cut(s) 373, 498, 540, 553, 670
BlsI GCNGC 5 cut(s) 374, 499, 541, 554, 671
Bme1390I CCNGG 8 cut(s) 83, 265, 427, 462, 463, 481, 482, 521
Bme18I GGWCC 2 cut(s) 379, 812
BmeRI GACNNNNNGTC 1 cut(s) 562
BmeT110I CYCGRG 4 cut(s) 461, 480, 583, 761
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 4 cut(s) 261, 379, 678, 812
BmiI GGNNCC 7 cut(s) 7, 100, 381, 513, 679, 785, 813
BmrFI CCNGG 8 cut(s) 83, 265, 427, 462, 463, 481, 482, 521
BpmI CTGGAG 2 cut(s) 286, 665
BpuMI CCSGG 6 cut(s) 83, 462, 463, 481, 482, 521
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 135, 629
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 5 cut(s) 81, 425, 448, 461, 480
BsaXI ACNNNNNCTCC 8 cut(s) 59, 89, 223, 253, 518, 548, 792, 822
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 250, 293, 430, 480, 487, 793
Bse118I RCCGGY 3 cut(s) 177, 430, 500
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 265, 427
BseDI CCNNGG 5 cut(s) 81, 425, 448, 461, 480
BseGI GGATG 3 cut(s) 579, 694, 708
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 7 cut(s) 217, 250, 293, 430, 480, 487, 793
BseMII CTCAG 1 cut(s) 654
BseX3I CGGCCG 1 cut(s) 498
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 4 cut(s) 375, 539, 614, 637
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 6 cut(s) 263, 279, 430, 500, 504, 680
BshNI GGYRCC 1 cut(s) 783
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 3 cut(s) 77, 417, 770
BsiHKCI CYCGRG 4 cut(s) 461, 480, 583, 761
BsiSI CCGG 9 cut(s) 83, 96, 178, 431, 462, 481, 501, 509, 521
BslFI GGGAC 2 cut(s) 194, 825
BslI CCNNNNNNNGG 7 cut(s) 217, 250, 293, 430, 480, 487, 793
BsmAI GTCTC 3 cut(s) 165, 470, 485
BsmBI CGTCTC 2 cut(s) 165, 485
BsmFI GGGAC 2 cut(s) 194, 825
BsnI GGCC 6 cut(s) 263, 279, 430, 500, 504, 680
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 4 cut(s) 461, 480, 583, 761
Bsp1286I GDGCHC 6 cut(s) 8, 77, 299, 417, 653, 770
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
BspANI GGCC 6 cut(s) 263, 279, 430, 500, 504, 680
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 4 cut(s) 375, 539, 614, 637
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 7 cut(s) 7, 100, 381, 513, 679, 785, 813
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 2 cut(s) 473, 552
BspT107I GGYRCC 1 cut(s) 783
BspTNI GGTCTC 1 cut(s) 470
BsrBI CCGCTC 1 cut(s) 669
BsrFI RCCGGY 3 cut(s) 177, 430, 500
BsrGI TGTACA 1 cut(s) 187
BssAI RCCGGY 3 cut(s) 177, 430, 500
BssECI CCNNGG 5 cut(s) 81, 425, 448, 461, 480
BssMI GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
BssNI GRCGYC 2 cut(s) 135, 629
BssSI CACGAG 1 cut(s) 70
Bst2BI CACGAG 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 265, 427
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 188, 717
BstACI GRCGYC 2 cut(s) 135, 629
BstAUI TGTACA 1 cut(s) 187
BstC8I GCNNGC 6 cut(s) 179, 183, 432, 502, 506, 770
BstDEI CTNAG 2 cut(s) 663, 822
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 3 cut(s) 579, 694, 708
BstFNI CGCG 4 cut(s) 375, 539, 614, 637
BstKTI GATC 8 cut(s) 49, 153, 399, 468, 547, 579, 748, 828
BstMAI GTCTC 3 cut(s) 165, 470, 485
BstMBI GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNI CCWGG 2 cut(s) 265, 427
BstSCI CCNGG 8 cut(s) 81, 263, 425, 460, 461, 479, 480, 519
BstUI CGCG 4 cut(s) 375, 539, 614, 637
BstX2I RGATCY 2 cut(s) 544, 825
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 2 cut(s) 544, 825
BstZI CGGCCG 1 cut(s) 498
BsuRI GGCC 6 cut(s) 263, 279, 430, 500, 504, 680
BtgI CCRYGG 1 cut(s) 448
BtrI CACGTC 1 cut(s) 694
BtsCI GGATG 3 cut(s) 579, 694, 708
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 6 cut(s) 179, 183, 432, 502, 506, 770
CciI TCATGA 1 cut(s) 33
Cfr10I RCCGGY 3 cut(s) 177, 430, 500
Cfr13I GGNCC 4 cut(s) 261, 379, 678, 812
Cfr9I CCCGGG 2 cut(s) 461, 480
CseI GACGC 1 cut(s) 577
Csp6I GTAC 2 cut(s) 188, 274
CviAII CATG 3 cut(s) 34, 258, 619
CviQI GTAC 2 cut(s) 188, 274
DdeI CTNAG 2 cut(s) 663, 822
DpnI GATC 8 cut(s) 48, 152, 398, 467, 546, 578, 747, 827
DpnII GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
DriI GACNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 4 cut(s) 277, 428, 498, 502
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 2 cut(s) 188, 717
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 2 cut(s) 188, 717
EciI GGCGGA 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 498
Eco24I GRGCYC 4 cut(s) 8, 77, 299, 653
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 379, 812
Eco52I CGGCCG 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 75
Eco88I CYCGRG 4 cut(s) 461, 480, 583, 761
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 263, 425
EcoT38I GRGCYC 4 cut(s) 8, 77, 299, 653
Esp3I CGTCTC 2 cut(s) 165, 485
FaeI CATG 3 cut(s) 37, 261, 622
FaiI YATR 3 cut(s) 35, 259, 620
FaqI GGGAC 2 cut(s) 194, 825
FatI CATG 3 cut(s) 33, 257, 618
FauI CCCGC 5 cut(s) 130, 174, 252, 630, 691
FbaI TGATCA 3 cut(s) 46, 396, 576
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 5 cut(s) 373, 498, 540, 553, 670
FokI GGATG 3 cut(s) 566, 701, 715
FriOI GRGCYC 4 cut(s) 8, 77, 299, 653
FseI GGCCGGCC 1 cut(s) 504
Fsp4HI GCNGC 5 cut(s) 373, 498, 540, 553, 670
FspBI CTAG 1 cut(s) 438
GluI GCNGC 5 cut(s) 373, 498, 540, 553, 670
GsuI CTGGAG 2 cut(s) 286, 665
HaeIII GGCC 6 cut(s) 263, 279, 430, 500, 504, 680
HapII CCGG 9 cut(s) 83, 96, 178, 431, 462, 481, 501, 509, 521
HgaI GACGC 1 cut(s) 577
Hin1I GRCGYC 2 cut(s) 135, 629
Hin1II CATG 3 cut(s) 37, 261, 622
HinfI GANTC 3 cut(s) 254, 563, 731
HpaII CCGG 9 cut(s) 83, 96, 178, 431, 462, 481, 501, 509, 521
HphI GGTGA 4 cut(s) 291, 391, 406, 586
Hpy166II GTNNAC 3 cut(s) 142, 379, 595
Hpy188I TCNGA 3 cut(s) 253, 412, 825
Hpy188III TCNNGA 5 cut(s) 34, 50, 438, 583, 712
Hpy8I GTNNAC 3 cut(s) 142, 379, 595
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 3 cut(s) 135, 629, 693
HpyCH4V TGCA 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 2 cut(s) 663, 822
HpySE526I ACGT 3 cut(s) 135, 629, 693
Hsp92I GRCGYC 2 cut(s) 135, 629
Hsp92II CATG 3 cut(s) 37, 261, 622
KroI GCCGGC 3 cut(s) 177, 430, 500
KroNI GCCGGC 3 cut(s) 179, 432, 502
Ksp22I TGATCA 3 cut(s) 46, 396, 576
Kzo9I GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
LmnI GCTCC 4 cut(s) 11, 80, 98, 104
MaeI CTAG 1 cut(s) 438
MaeII ACGT 3 cut(s) 135, 629, 693
MaeIII GTNAC 1 cut(s) 227
MalI GATC 8 cut(s) 48, 152, 398, 467, 546, 578, 747, 827
MbiI CCGCTC 1 cut(s) 669
MboI GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
MboII GAAGA 3 cut(s) 10, 205, 734
MflI RGATCY 2 cut(s) 544, 825
MhlI GDGCHC 6 cut(s) 8, 77, 299, 417, 653, 770
MlyI GAGTC 2 cut(s) 572, 740
MmeI TCCRAC 1 cut(s) 231
MroNI GCCGGC 3 cut(s) 177, 430, 500
MslI CAYNNNNRTG 2 cut(s) 392, 789
MspI CCGG 9 cut(s) 83, 96, 178, 431, 462, 481, 501, 509, 521
MspR9I CCNGG 8 cut(s) 83, 265, 427, 462, 463, 481, 482, 521
MvaI CCWGG 2 cut(s) 265, 427
MvnI CGCG 4 cut(s) 375, 539, 614, 637
MwoI GCNNNNNNNGC 1 cut(s) 450
NaeI GCCGGC 3 cut(s) 179, 432, 502
NciI CCSGG 6 cut(s) 83, 462, 463, 481, 482, 521
NdeII GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
NgoMIV GCCGGC 3 cut(s) 177, 430, 500
NlaIII CATG 3 cut(s) 37, 261, 622
NlaIV GGNNCC 7 cut(s) 7, 100, 381, 513, 679, 785, 813
NmeAIII GCCGAG 1 cut(s) 499
OliI CACNNNNGTG 2 cut(s) 392, 789
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 787
PdiI GCCGGC 3 cut(s) 179, 432, 502
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 2 cut(s) 137, 631
PkrI GCNGC 5 cut(s) 374, 499, 541, 554, 671
PleI GAGTC 2 cut(s) 571, 739
PpsI GAGTC 2 cut(s) 571, 739
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 263, 425
PspGI CCWGG 2 cut(s) 263, 425
PspN4I GGNNCC 7 cut(s) 7, 100, 381, 513, 679, 785, 813
PspPI GGNCC 4 cut(s) 261, 379, 678, 812
PsuI RGATCY 2 cut(s) 544, 825
PsyI GACNNNGTC 2 cut(s) 137, 631
RigI GGCCGGCC 1 cut(s) 504
RsaI GTAC 2 cut(s) 189, 275
RsaNI GTAC 2 cut(s) 188, 274
RseI CAYNNNNRTG 2 cut(s) 392, 789
SacI GAGCTC 1 cut(s) 77
SatI GCNGC 5 cut(s) 373, 498, 540, 553, 670
Sau3AI GATC 8 cut(s) 46, 150, 396, 465, 544, 576, 745, 825
Sau96I GGNCC 4 cut(s) 261, 379, 678, 812
SchI GAGTC 2 cut(s) 572, 740
ScrFI CCNGG 8 cut(s) 83, 265, 427, 462, 463, 481, 482, 521
SduI GDGCHC 6 cut(s) 8, 77, 299, 417, 653, 770
SinI GGWCC 2 cut(s) 379, 812
SmaI CCCGGG 2 cut(s) 463, 482
SmiMI CAYNNNNRTG 2 cut(s) 392, 789
SspMI CTAG 1 cut(s) 438
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 8 cut(s) 81, 263, 425, 460, 461, 479, 480, 519
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 3 cut(s) 138, 632, 696
TatI WGTACW 1 cut(s) 187
TauI GCSGC 5 cut(s) 375, 500, 542, 555, 672
TfiI GAWTC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 22, 246
TspGWI ACGGA 1 cut(s) 701
TspMI CCCGGG 2 cut(s) 461, 480
Tth111I GACNNNGTC 2 cut(s) 137, 631
VpaK11BI GGWCC 2 cut(s) 379, 812
XbaI TCTAGA 1 cut(s) 437
XmaI CCCGGG 2 cut(s) 461, 480
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 1 cut(s) 438
ZraI GACGTC 2 cut(s) 136, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.