Rorug04G0457800
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011269
Physical Location & Seq
Reverse (-)
61096677 .. 61104706
8030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0457800.1

Sequence Viewer

Length: 2397 bp
ATGTTGAAATTCTTGAATCGAGTGGTGGGTGGATCTGGGAGCGGACCCAAAGATCTGCCATACAACATCGGCGAGCCCTATCCCTCTGCTTGGGGCTCTTGGACTCACTTCAGGGGCACCTCCAAGGACGATGGGTCTCCGGTGTCTATATTTTCCATTAGTGGAAGTAATGCTCAGGATGGCCATTTGGCCGCCGCTCGCAATGGCGTCAAGCGTCTCCGTACTGTCAGACATCCCAACATCTTATCATTTCTCCACAGTACTGAAACCGAAACCATGGATGCTTCTACCACCAAGCACACTATCTACATTGTCACCGAGCCTGTTATGCCACTCTCTGAGAAGATCAAAGAACTCAGTTTACAAAACATCCAAAGGGATGAATATTACGCTTGGGGCCTGCACCAGATTGCTAAAGCTGTTAGTTTCTTAAATAATGATTGTAAACTGGTTCATTCCAATGTTTGTGTTGCCAGTGTGGTTGTAACTCAAACGTTGGACTGGAAGCTGCATGCTTTTGATGTTCTTTCCGAGTTTGATGGCAGCAATGAGGCTTCTGCTGGACCAATGCTGCAATATGCCTGGCTTGTTGGATCACAATACAAACCTGTGGAGTTGTTGAAGTCTGATTGGGCGGCAGTCAGAAAGTCTCCACCATGGGCCATTGATTCTTGGGGCTTGGGCTGTCTTATCTACGAGCTCTTCTCTGGTCTGAAGTTAAGCAAAACAGAGGAGCTGCGAAACACTGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCCTGAGTGCCATACCTTCTCGTAGGTTGAATACGTCGAAGCTTATAGAAAACAGTGCCTATTTTCAAAATAAGTTGGTGGATACAATACACTTCATGGAAATTCTAAATTTGAAGGATAGTGTAGAGAAAGATACTTTCTTCCGCAAGCTTCCGAATTTGGCAGAGCAGCTACCTCGTCAAATTGTGCTGAAAAAGTTACTTCCTTTATTAGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCACCTGCTTTGACTGCATTGTTAAAAATGGGTTCTTGGCTTTCAACTGAAGAATTTAGTGTGAAGTTGCTGCCGACCATTGTGAAACTGTTTGCTTCCAATGACCGAGCTATTCGAGTTGGTCTTTTGCAACATGTTGATCAGTTTGGAGAATCATTATCAGCACAAATTGTTGATGAGCAAGTTTATCCCCACGTTGCTACCGGTTTCTCTGATACATCTGCTTGTCTTCGGGAACTGACTCTAAAGTCGATGCTCGTTCTCGCTCCCAAGCTTTCTCAACGTACCATTTCAGGGTCGTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCTGCAATTAGAACAAATACTACTATATTACTAGGGAACATTGCAAGCCACCTGAATGAAGGGACAAGGAAAAGAGTTTTGATTAATGCATTTACTGTTCGTGCATTACGGGATAGTTTCTCACCTGCCCGAGGAGCAGGGATTATGGCTTTATGTGCAACCAGCTCTTATTATGACATCACTGAGATTTCAACTAGGATCCTCCCAAATGTTGTTGTTCTAATTATTGACCCTGACAATGATGTTCGATCGAAGGCATTTCAAGCAGTTGATCAGTTTCTACAGATAGTGAAGCAAACCTATGAGAAGACAAATTCAGGAGATGCTGCTGGGGCAGCAGGTGACGGAATGTCATCAATGCCAGGAAATGCTAGTTTACTGGGATGGGCAATGAGCTCCTTAACTCTCAAGGGTAAACCAGCTGAACAAGCTCCGCTTGCACTTGTCAATACCAGTGCATCTCTTACTAAAACGACTTCCAATGACAGCTCAGCGATGGATACTCCAACCACAGCACCTGCCCATGTAAGTTCGACAACAGATTTCCCAGATCAACATGTACCCGAGTCCCCTACGTCCACAGATGGCTGGGGAGAGCTAGAAAATGGAATACAGGAGGAGCATGAAAGTGACAAGGATGGTTGGGATGATATTGAACCCCTTGAAGAGCCAACACCACCTCCAGCTCTTGCTAACATTCAAGCTGCTCAGAAGCGGCCGGTCTCGCAATCGCAGCCAAAGCCAGCAAGTTTGCGACCCAAAAACACGGCCAAAGTTATCAAAGATGAAGATGATGATTTGTGGGGCTCCATTGCCGCCCCAGCACCCAAAACAAGTTCAAAGGCTTTGAATTTGAAAACAAGTCGATCAGTTGATGATGATGATGATGATCCATGGGCTGCAATCGCCGCTCCCCTACCTACTACTAAGGCAAGACCCTTAGCGGCTGGCAGAGGCCGAGGGGCTAAACCCGCTGCTCCAAAATTGGGTGCACAGAGGATAAATCGGACTTCTTCATCTGGGGTGTAA

Protein Analysis

798

Amino Acids

86.68

Weight (kDa)

6.46

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 37 - 245 7.4e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 4 cut(s) 1042, 1501, 1697, 1894
AasI GACNNNNNNGTC 1 cut(s) 1276
Acc36I ACCTGC 5 cut(s) 1042, 1378, 1501, 1697, 1894
AccB1I GGYRCC 1 cut(s) 116
AccBSI CCGCTC 3 cut(s) 42, 197, 2279
AclI AACGTT 1 cut(s) 494
AclWI GGATC 5 cut(s) 40, 601, 1561, 1574, 2252
AcoI YGGCCR 4 cut(s) 181, 189, 2084, 2136
AcsI RAATTY 8 cut(s) 8, 888, 895, 943, 1013, 1082, 1681, 2218
AcuI CTGAAG 3 cut(s) 94, 734, 1098
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 4 cut(s) 223, 262, 1315, 1929
AfiI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2354
AflIII ACRYGT 2 cut(s) 1162, 1924
AgeI ACCGGT 1 cut(s) 1232
AhdI GACNNNNNGTC 2 cut(s) 133, 1717
AjnI CCWGG 2 cut(s) 581, 1729
Alw21I GWGCWC 3 cut(s) 702, 1766, 2362
Alw26I GTCTC 5 cut(s) 141, 221, 654, 768, 2095
Alw44I GTGCAC 1 cut(s) 2358
AlwI GGATC 5 cut(s) 40, 601, 1561, 1574, 2252
AlwNI CAGNNNCTG 2 cut(s) 1028, 1886
Ama87I CYCGRG 2 cut(s) 1497, 1931
AoxI GGCC 7 cut(s) 181, 189, 397, 660, 2084, 2136, 2323
ApaLI GTGCAC 1 cut(s) 2358
ApoI RAATTY 8 cut(s) 8, 888, 895, 943, 1013, 1082, 1681, 2218
AseI ATTAAT 1 cut(s) 1452
AsiGI ACCGGT 1 cut(s) 1232
AspS9I GGNCC 4 cut(s) 44, 397, 563, 660
AsuHPI GGTGA 3 cut(s) 307, 1482, 1721
AvaI CYCGRG 2 cut(s) 1497, 1931
AvaII GGWCC 2 cut(s) 44, 563
BaeGI GKGCMC 2 cut(s) 119, 2362
BalI TGGCCA 1 cut(s) 183
BamHI GGATCC 1 cut(s) 1566
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 5 cut(s) 78, 98, 702, 1766, 2177
BbsI GAAGAC 2 cut(s) 1250, 1682
Bbv12I GWGCWC 3 cut(s) 702, 1766, 2362
BccI CCATC 7 cut(s) 125, 173, 533, 1746, 1858, 1946, 2000
BceAI ACGGC 1 cut(s) 2151
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 2 cut(s) 583, 1731
BciVI GTATCC 2 cut(s) 862, 1861
BclI TGATCA 2 cut(s) 1168, 1639
BcoDI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BfaI CTAG 4 cut(s) 1400, 1563, 1740, 1967
BfmI CTRYAG 1 cut(s) 1649
BfuAI ACCTGC 5 cut(s) 1042, 1378, 1501, 1697, 1894
BfuI GTATCC 2 cut(s) 862, 1861
BglII AGATCT 1 cut(s) 52
BlpI GCTNAGC 1 cut(s) 1858
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 583, 1731
Bme18I GGWCC 2 cut(s) 44, 563
BmeRI GACNNNNNGTC 2 cut(s) 133, 1717
BmeT110I CYCGRG 2 cut(s) 1497, 1931
BmgT120I GGNCC 4 cut(s) 44, 397, 563, 660
BmiI GGNNCC 6 cut(s) 46, 118, 398, 788, 1568, 2176
BmrFI CCNGG 2 cut(s) 583, 1731
BmrI ACTGGG 1 cut(s) 1757
BmsI GCATC 4 cut(s) 271, 1272, 1681, 1835
BmuI ACTGGG 1 cut(s) 1757
BpiI GAAGAC 2 cut(s) 1250, 1682
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 1 cut(s) 2034
Bpu10I CCTNAGC 2 cut(s) 174, 2308
Bpu1102I GCTNAGC 1 cut(s) 1858
BpuEI CTTGAG 1 cut(s) 1760
BsaBI GATNNNNATC 1 cut(s) 2256
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 2 cut(s) 141, 2095
BsaJI CCNNGG 6 cut(s) 123, 276, 656, 1498, 2261, 2326
BsaWI WCCGGW 2 cut(s) 139, 1232
BsaXI ACNNNNNCTCC 2 cut(s) 2032, 2062
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2354
Bse118I RCCGGY 2 cut(s) 1232, 2086
Bse1I ACTGG 5 cut(s) 453, 474, 506, 1752, 1821
Bse3DI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2178
Bse8I GATNNNNATC 1 cut(s) 2256
BseBI CCWGG 2 cut(s) 583, 1731
BseDI CCNNGG 6 cut(s) 123, 276, 656, 1498, 2261, 2326
BseGI GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BseJI GATNNNNATC 1 cut(s) 2256
BseLI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2354
BseMI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2178
BseMII CTCAG 7 cut(s) 188, 330, 370, 782, 1542, 1872, 2090
BseNI ACTGG 5 cut(s) 453, 474, 506, 1752, 1821
BseRI GAGGAG 3 cut(s) 746, 1515, 2000
BseSI GKGCMC 2 cut(s) 119, 2362
BseX3I CGGCCG 1 cut(s) 2084
BseYI CCCAGC 3 cut(s) 1697, 1956, 2188
BsgI GTGCAG 2 cut(s) 386, 1014
Bsh1285I CGRYCG 2 cut(s) 1619, 2087
BshFI GGCC 7 cut(s) 183, 191, 399, 662, 2086, 2138, 2325
BshNI GGYRCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 1232
BsiEI CGRYCG 2 cut(s) 1619, 2087
BsiHKAI GWGCWC 3 cut(s) 702, 1766, 2362
BsiHKCI CYCGRG 2 cut(s) 1497, 1931
BsiSI CCGG 3 cut(s) 140, 1233, 2087
BslFI GGGAC 2 cut(s) 1444, 1921
BslI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2354
BsmAI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 1444, 1921
BsnI GGCC 7 cut(s) 183, 191, 399, 662, 2086, 2138, 2325
Bso31I GGTCTC 2 cut(s) 141, 2095
BsoBI CYCGRG 2 cut(s) 1497, 1931
Bsp1286I GDGCHC 7 cut(s) 78, 98, 119, 702, 1766, 2177, 2362
Bsp1720I GCTNAGC 1 cut(s) 1858
Bsp19I CCATGG 3 cut(s) 276, 656, 2261
BspANI GGCC 7 cut(s) 183, 191, 399, 662, 2086, 2138, 2325
BspCNI CTCAG 7 cut(s) 187, 331, 369, 783, 1543, 1871, 2089
BspLI GGNNCC 6 cut(s) 46, 118, 398, 788, 1568, 2176
BspMI ACCTGC 5 cut(s) 1042, 1378, 1501, 1697, 1894
BspPI GGATC 5 cut(s) 40, 601, 1561, 1574, 2252
BspQI GCTCTTC 2 cut(s) 707, 2028
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 2 cut(s) 141, 2095
BsrBI CCGCTC 3 cut(s) 42, 197, 2279
BsrDI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2178
BsrFI RCCGGY 2 cut(s) 1232, 2086
BsrI ACTGG 5 cut(s) 453, 474, 506, 1752, 1821
BssAI RCCGGY 2 cut(s) 1232, 2086
BssECI CCNNGG 6 cut(s) 123, 276, 656, 1498, 2261, 2326
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 4 cut(s) 123, 276, 656, 2261
Bst2UI CCWGG 2 cut(s) 583, 1731
Bst4CI ACNGT 5 cut(s) 226, 260, 842, 1119, 1465
Bst6I CTCTTC 2 cut(s) 707, 2028
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 9 cut(s) 74, 199, 401, 513, 935, 1414, 1806, 2112, 2317
BstDEI CTNAG 9 cut(s) 174, 339, 356, 791, 1551, 1858, 2076, 2295, 2308
BstDSI CCRYGG 3 cut(s) 276, 656, 2261
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BstMAI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BstMCI CGRYCG 2 cut(s) 1619, 2087
BstNI CCWGG 2 cut(s) 583, 1731
BstNSI RCATGY 3 cut(s) 515, 1166, 1928
BstSCI CCNGG 2 cut(s) 581, 1729
BstSFI CTRYAG 1 cut(s) 1649
BstSLI GKGCMC 2 cut(s) 119, 2362
BstV2I GAAGAC 2 cut(s) 1250, 1682
BstX2I RGATCY 3 cut(s) 32, 52, 1566
BstYI RGATCY 3 cut(s) 32, 52, 1566
BstZI CGGCCG 1 cut(s) 2084
BsuI GTATCC 2 cut(s) 862, 1861
BsuRI GGCC 7 cut(s) 183, 191, 399, 662, 2086, 2138, 2325
BtgI CCRYGG 3 cut(s) 276, 656, 2261
BtgZI GCGATG 1 cut(s) 1877
BtsCI GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 5 cut(s) 481, 744, 847, 1548, 1828
BveI ACCTGC 5 cut(s) 1042, 1378, 1501, 1697, 1894
Cac8I GCNNGC 9 cut(s) 74, 199, 401, 513, 935, 1414, 1806, 2112, 2317
CaiI CAGNNNCTG 2 cut(s) 1028, 1886
Cfr10I RCCGGY 2 cut(s) 1232, 2086
Cfr13I GGNCC 4 cut(s) 44, 397, 563, 660
CseI GACGC 2 cut(s) 196, 203
Csp6I GTAC 4 cut(s) 222, 261, 1314, 1928
CspAI ACCGGT 1 cut(s) 1232
CviAII CATG 9 cut(s) 277, 512, 657, 883, 1163, 1892, 1925, 1991, 2262
CviQI GTAC 4 cut(s) 222, 261, 1314, 1928
DdeI CTNAG 9 cut(s) 174, 339, 356, 791, 1551, 1858, 2076, 2295, 2308
DrdI GACNNNNNNGTC 1 cut(s) 1276
DriI GACNNNNNGTC 2 cut(s) 133, 1717
DseDI GACNNNNNNGTC 1 cut(s) 1276
EaeI YGGCCR 4 cut(s) 181, 189, 2084, 2136
EagI CGGCCG 1 cut(s) 2084
Eam1104I CTCTTC 2 cut(s) 707, 2028
Eam1105I GACNNNNNGTC 2 cut(s) 133, 1717
EarI CTCTTC 2 cut(s) 707, 2028
Ecl136II GAGCTC 2 cut(s) 700, 1764
EclXI CGGCCG 1 cut(s) 2084
Eco130I CCWWGG 4 cut(s) 123, 276, 656, 2261
Eco24I GRGCYC 5 cut(s) 78, 98, 702, 1766, 2177
Eco31I GGTCTC 2 cut(s) 141, 2095
Eco47I GGWCC 2 cut(s) 44, 563
Eco52I CGGCCG 1 cut(s) 2084
Eco53kI GAGCTC 2 cut(s) 700, 1764
Eco57I CTGAAG 3 cut(s) 94, 734, 1098
Eco88I CYCGRG 2 cut(s) 1497, 1931
EcoICRI GAGCTC 2 cut(s) 700, 1764
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 581, 1729
EcoT14I CCWWGG 4 cut(s) 123, 276, 656, 2261
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 5 cut(s) 78, 98, 702, 1766, 2177
ErhI CCWWGG 4 cut(s) 123, 276, 656, 2261
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 9 cut(s) 280, 515, 660, 886, 1166, 1895, 1928, 1994, 2265
FalI AAGNNNNNCTT 2 cut(s) 1788, 1820
FaqI GGGAC 2 cut(s) 1444, 1921
FatI CATG 9 cut(s) 276, 511, 656, 882, 1162, 1891, 1924, 1990, 2261
FauI CCCGC 1 cut(s) 2347
FbaI TGATCA 2 cut(s) 1168, 1639
FokI GGATG 8 cut(s) 191, 219, 293, 356, 392, 1764, 2018, 2027
FriOI GRGCYC 5 cut(s) 78, 98, 702, 1766, 2177
FspBI CTAG 4 cut(s) 1400, 1563, 1740, 1967
GsaI CCCAGC 3 cut(s) 1701, 1960, 2192
GsuI CTGGAG 1 cut(s) 2034
HaeIII GGCC 7 cut(s) 183, 191, 399, 662, 2086, 2138, 2325
HapII CCGG 3 cut(s) 140, 1233, 2087
HgaI GACGC 2 cut(s) 196, 203
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 9 cut(s) 280, 515, 660, 886, 1166, 1895, 1928, 1994, 2265
HindIII AAGCTT 3 cut(s) 827, 935, 1301
HinfI GANTC 6 cut(s) 16, 103, 668, 1181, 1270, 1934
HpaII CCGG 3 cut(s) 140, 1233, 2087
HphI GGTGA 3 cut(s) 307, 1482, 1721
Hpy166II GTNNAC 6 cut(s) 362, 446, 1745, 1784, 1947, 2360
Hpy188III TCNNGA 6 cut(s) 13, 176, 773, 790, 1262, 1686
Hpy8I GTNNAC 6 cut(s) 362, 446, 1745, 1784, 1947, 2360
Hpy99I CGWCG 1 cut(s) 826
HpyAV CCTTC 4 cut(s) 813, 895, 1421, 1615
HpyCH4III ACNGT 5 cut(s) 226, 260, 842, 1119, 1465
HpyCH4IV ACGT 5 cut(s) 494, 821, 1224, 1312, 1943
HpyF3I CTNAG 9 cut(s) 174, 339, 356, 791, 1551, 1858, 2076, 2295, 2308
HpySE526I ACGT 5 cut(s) 494, 821, 1224, 1312, 1943
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 9 cut(s) 280, 515, 660, 886, 1166, 1895, 1928, 1994, 2265
Ksp22I TGATCA 2 cut(s) 1168, 1639
LguI GCTCTTC 2 cut(s) 707, 2028
LweI GCATC 4 cut(s) 271, 1272, 1681, 1835
MaeI CTAG 4 cut(s) 1400, 1563, 1740, 1967
MaeII ACGT 5 cut(s) 494, 821, 1224, 1312, 1943
MaeIII GTNAC 6 cut(s) 313, 484, 984, 1350, 1709, 1997
MbiI CCGCTC 3 cut(s) 42, 197, 2279
MflI RGATCY 3 cut(s) 32, 52, 1566
MhlI GDGCHC 7 cut(s) 78, 98, 119, 702, 1766, 2177, 2362
MlsI TGGCCA 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 3 cut(s) 97, 1264, 1943
MmeI TCCRAC 3 cut(s) 477, 571, 1898
Mox20I TGGCCA 1 cut(s) 183
Mph1103I ATGCAT 1 cut(s) 1459
MscI TGGCCA 1 cut(s) 183
MseI TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
MslI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
Msp20I TGGCCA 1 cut(s) 183
MspA1I CMGCKG 4 cut(s) 784, 1025, 1790, 2342
MspI CCGG 3 cut(s) 140, 1233, 2087
MspR9I CCNGG 2 cut(s) 583, 1731
MvaI CCWGG 2 cut(s) 583, 1731
NcoI CCATGG 3 cut(s) 276, 656, 2261
NlaIII CATG 9 cut(s) 280, 515, 660, 886, 1166, 1895, 1928, 1994, 2265
NlaIV GGNNCC 6 cut(s) 46, 118, 398, 788, 1568, 2176
NmeAIII GCCGAG 1 cut(s) 2351
NmuCI GTSAC 3 cut(s) 313, 1709, 1997
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 3 cut(s) 515, 1166, 1928
PaeI GCATGC 1 cut(s) 515
PaqCI CACCTGC 4 cut(s) 1042, 1501, 1697, 1894
PciI ACATGT 2 cut(s) 1162, 1924
PciSI GCTCTTC 2 cut(s) 707, 2028
PfeI GAWTC 3 cut(s) 16, 668, 1181
PinAI ACCGGT 1 cut(s) 1232
Ple19I CGATCG 1 cut(s) 1619
PleI GAGTC 3 cut(s) 97, 1264, 1942
PpsI GAGTC 3 cut(s) 97, 1264, 1942
PscI ACATGT 2 cut(s) 1162, 1924
PshBI ATTAAT 1 cut(s) 1452
Psp124BI GAGCTC 2 cut(s) 702, 1766
Psp1406I AACGTT 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 581, 1729
PspFI CCCAGC 3 cut(s) 1697, 1956, 2188
PspGI CCWGG 2 cut(s) 581, 1729
PspN4I GGNNCC 6 cut(s) 46, 118, 398, 788, 1568, 2176
PspPI GGNCC 4 cut(s) 44, 397, 563, 660
PsrI GAACNNNNNNTAC 4 cut(s) 345, 377, 1372, 1404
PstNI CAGNNNCTG 2 cut(s) 1028, 1886
PsuI RGATCY 3 cut(s) 32, 52, 1566
PvuI CGATCG 1 cut(s) 1619
PvuII CAGCTG 2 cut(s) 1025, 1790
RsaI GTAC 4 cut(s) 223, 262, 1315, 1929
RsaNI GTAC 4 cut(s) 222, 261, 1314, 1928
RseI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
SacI GAGCTC 2 cut(s) 702, 1766
SapI GCTCTTC 2 cut(s) 707, 2028
SaqAI TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
Sau96I GGNCC 4 cut(s) 44, 397, 563, 660
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 3 cut(s) 97, 1264, 1943
ScrFI CCNGG 2 cut(s) 583, 1731
SduI GDGCHC 7 cut(s) 78, 98, 119, 702, 1766, 2177, 2362
SfaNI GCATC 4 cut(s) 271, 1272, 1681, 1835
SfcI CTRYAG 1 cut(s) 1649
SinI GGWCC 2 cut(s) 44, 563
SmiMI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
SmlI CTYRAG 1 cut(s) 1775
SmoI CTYRAG 1 cut(s) 1775
SphI GCATGC 1 cut(s) 515
SspI AATATT 1 cut(s) 386
SspMI CTAG 4 cut(s) 1400, 1563, 1740, 1967
SstI GAGCTC 2 cut(s) 702, 1766
StyD4I CCNGG 2 cut(s) 581, 1729
StyI CCWWGG 4 cut(s) 123, 276, 656, 2261
TaaI ACNGT 5 cut(s) 226, 260, 842, 1119, 1465
TaiI ACGT 5 cut(s) 497, 824, 1227, 1315, 1946
TaqI TCGA 8 cut(s) 19, 824, 1144, 1280, 1615, 1619, 1901, 2233
TaqII GACCGA 1 cut(s) 1149
TatI WGTACW 1 cut(s) 260
TauI GCSGC 8 cut(s) 194, 197, 638, 787, 2086, 2186, 2279, 2315
TfiI GAWTC 3 cut(s) 16, 668, 1181
Tru1I TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
Tru9I TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
TscAI CASTG 5 cut(s) 481, 751, 847, 1555, 1828
TseFI GTSAC 3 cut(s) 313, 1709, 1997
Tsp45I GTSAC 3 cut(s) 313, 1709, 1997
TspDTI ATGAA 8 cut(s) 396, 443, 871, 1377, 1440, 2007, 2169, 2373
TspGWI ACGGA 2 cut(s) 209, 1728
TspRI CASTG 5 cut(s) 481, 751, 847, 1555, 1828
VneI GTGCAC 1 cut(s) 2358
VpaK11BI GGWCC 2 cut(s) 44, 563
VspI ATTAAT 1 cut(s) 1452
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 8 cut(s) 8, 888, 895, 943, 1013, 1082, 1681, 2218
XceI RCATGY 3 cut(s) 515, 1166, 1928
XspI CTAG 4 cut(s) 1400, 1563, 1740, 1967
ZrmI AGTACT 1 cut(s) 262
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.