Rw5G007780
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011269
Physical Location & Seq
Forward (+)
8288884 .. 8290466
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007780.1

Sequence Viewer

Length: 822 bp
ATGGGCTCCCCCACATTCTTCAATAAACACTTCATGAGTGATATTGATCACGACGATTACAACACCACCACGAGCTCCACCCCGGAAAACAGCTCCGGCTCCCCTCCTCCCGCCTTCAACGACGTCGTCTACTCCAAGATCGCTTCGTCTCCCAAGAAAAGTAGCCGGCGGGCTGTACAGAAGAGAGTGGTGTCAATCCCTATCAAGGAAAACAGTAACACAAACACTCCCCCGCCGTCGGATTCATGGGCCTGGAGAAAGTACGGCCAGAAACCCATCAAGGGCTCACCTTACCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAGGGGTGTCCAGCGAGGAAGCAGGTGGAGAGAAGCCGCGTGGACCCCACCACGCTGGTGATCACCTACTCGTCAGAGCACAACCACCCCTGGCCGGCTTCTAGAAGCCACCATGGCACGAAACCCGGGATCAAAACCGAGACCCGGGAAGAGGAGACGGCGGCCGGCCAGCCGGAACCCGACCCGGAGGAGAGGCGGTTCGCGGCGGATCTGGGCGGCGGCGACGAGTCGTCGCTGGTGATCATCCCGAGCGTCGTGGACGAGTTCGGGTGGTTCGCGGACATGGAAACGACGTCGTCGCGGGTGCTGGAGAGCCCCATTTTCGCTGAGAGCGGCGGTGGGGCCGACATGGATGACGTGGCGGGGATGGTGTTCCCGATGGGCGAAGAGGACGAGTCGCTATTCGCCGATCTGGGCGAGTTGCCCGAGTGCTCGCTTGTGTTTCGGCACCGCCGTGTGGGAGGAGGAGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

273

Amino Acids

29.8

Weight (kDa)

5.44

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 81 - 138 7.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
AatII GACGTC 2 cut(s) 126, 620
Acc36I ACCTGC 1 cut(s) 337
AccB1I GGYRCC 1 cut(s) 771
AccBSI CCGCTC 1 cut(s) 657
AccI GTMKAC 1 cut(s) 129
AccII CGCG 4 cut(s) 363, 527, 602, 625
AclWI GGATC 2 cut(s) 461, 540
AcoI YGGCCR 4 cut(s) 265, 416, 486, 490
AcyI GRCGYC 2 cut(s) 123, 617
AfaI GTAC 2 cut(s) 177, 263
AfiI CCNNNNNNNGG 7 cut(s) 205, 238, 281, 418, 468, 475, 781
AgsI TTSAA 2 cut(s) 22, 118
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 2 cut(s) 251, 413
AleI CACNNNNGTG 2 cut(s) 380, 777
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 4 cut(s) 75, 93, 321, 809
AluI AGCT 4 cut(s) 75, 93, 321, 809
Alw21I GWGCWC 3 cut(s) 77, 405, 758
Alw26I GTCTC 3 cut(s) 153, 458, 473
AlwI GGATC 2 cut(s) 461, 540
Ama87I CYCGRG 4 cut(s) 449, 468, 571, 749
AoxI GGCC 6 cut(s) 249, 265, 416, 486, 490, 666
AspS9I GGNCC 4 cut(s) 249, 367, 666, 800
AsuC2I CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
AsuHPI GGTGA 4 cut(s) 279, 379, 394, 574
AvaI CYCGRG 4 cut(s) 449, 468, 571, 749
AvaII GGWCC 2 cut(s) 367, 800
BanI GGYRCC 1 cut(s) 771
BanII GRGCYC 4 cut(s) 8, 77, 287, 641
BauI CACGAG 1 cut(s) 70
Bbv12I GWGCWC 3 cut(s) 77, 405, 758
BccI CCATC 3 cut(s) 284, 685, 697
BceAI ACGGC 4 cut(s) 220, 280, 498, 762
BciT130I CCWGG 2 cut(s) 253, 415
BclI TGATCA 3 cut(s) 46, 384, 564
BcnI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
BcoDI GTCTC 3 cut(s) 153, 458, 473
BfaI CTAG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 438
BglII AGATCT 1 cut(s) 813
BisI GCNGC 6 cut(s) 361, 486, 528, 541, 544, 658
BlsI GCNGC 6 cut(s) 362, 487, 529, 542, 545, 659
Bme1390I CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
Bme18I GGWCC 2 cut(s) 367, 800
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 4 cut(s) 449, 468, 571, 749
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 4 cut(s) 249, 367, 666, 800
BmiI GGNNCC 7 cut(s) 7, 100, 369, 501, 667, 773, 801
BmrFI CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
BpmI CTGGAG 2 cut(s) 274, 653
BpuMI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
BsaBI GATNNNNATC 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 123, 617
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 5 cut(s) 81, 413, 436, 449, 468
BsaXI ACNNNNNCTCC 6 cut(s) 211, 241, 506, 536, 780, 810
Bsc4I CCNNNNNNNGG 7 cut(s) 205, 238, 281, 418, 468, 475, 781
Bse118I RCCGGY 3 cut(s) 165, 418, 488
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 253, 415
BseDI CCNNGG 5 cut(s) 81, 413, 436, 449, 468
BseGI GGATG 3 cut(s) 567, 682, 696
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 7 cut(s) 205, 238, 281, 418, 468, 475, 781
BseMII CTCAG 1 cut(s) 642
BseRI GAGGAG 5 cut(s) 96, 491, 527, 801, 804
BseX3I CGGCCG 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 4 cut(s) 363, 527, 602, 625
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 6 cut(s) 251, 267, 418, 488, 492, 668
BshNI GGYRCC 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 3 cut(s) 77, 405, 758
BsiHKCI CYCGRG 4 cut(s) 449, 468, 571, 749
BsiSI CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
BslFI GGGAC 1 cut(s) 813
BslI CCNNNNNNNGG 7 cut(s) 205, 238, 281, 418, 468, 475, 781
BsmAI GTCTC 3 cut(s) 153, 458, 473
BsmBI CGTCTC 2 cut(s) 153, 473
BsmFI GGGAC 1 cut(s) 813
BsnI GGCC 6 cut(s) 251, 267, 418, 488, 492, 668
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 4 cut(s) 449, 468, 571, 749
Bsp1286I GDGCHC 6 cut(s) 8, 77, 287, 405, 641, 758
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
Bsp19I CCATGG 1 cut(s) 436
BspANI GGCC 6 cut(s) 251, 267, 418, 488, 492, 668
BspCNI CTCAG 1 cut(s) 643
BspFNI CGCG 4 cut(s) 363, 527, 602, 625
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 7 cut(s) 7, 100, 369, 501, 667, 773, 801
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 461, 540
BspT107I GGYRCC 1 cut(s) 771
BspTNI GGTCTC 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 657
BsrFI RCCGGY 3 cut(s) 165, 418, 488
BsrGI TGTACA 1 cut(s) 175
BssAI RCCGGY 3 cut(s) 165, 418, 488
BssECI CCNNGG 5 cut(s) 81, 413, 436, 449, 468
BssMI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
BssNI GRCGYC 2 cut(s) 123, 617
BssSI CACGAG 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 253, 415
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 3 cut(s) 176, 468, 705
BstACI GRCGYC 2 cut(s) 123, 617
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 6 cut(s) 167, 171, 420, 490, 494, 758
BstDEI CTNAG 2 cut(s) 651, 810
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 567, 682, 696
BstFNI CGCG 4 cut(s) 363, 527, 602, 625
BstKTI GATC 8 cut(s) 49, 141, 387, 456, 535, 567, 736, 816
BstMAI GTCTC 3 cut(s) 153, 458, 473
BstMBI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNI CCWGG 2 cut(s) 253, 415
BstSCI CCNGG 8 cut(s) 81, 251, 413, 448, 449, 467, 468, 507
BstUI CGCG 4 cut(s) 363, 527, 602, 625
BstX2I RGATCY 2 cut(s) 532, 813
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 2 cut(s) 532, 813
BstZI CGGCCG 1 cut(s) 486
BsuRI GGCC 6 cut(s) 251, 267, 418, 488, 492, 668
BtgI CCRYGG 1 cut(s) 436
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 3 cut(s) 567, 682, 696
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 6 cut(s) 167, 171, 420, 490, 494, 758
CciI TCATGA 1 cut(s) 33
Cfr10I RCCGGY 3 cut(s) 165, 418, 488
Cfr13I GGNCC 4 cut(s) 249, 367, 666, 800
Cfr9I CCCGGG 2 cut(s) 449, 468
CseI GACGC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 176, 262
CviAII CATG 5 cut(s) 34, 246, 437, 607, 673
CviQI GTAC 2 cut(s) 176, 262
DdeI CTNAG 2 cut(s) 651, 810
DpnI GATC 8 cut(s) 48, 140, 386, 455, 534, 566, 735, 815
DpnII GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
DriI GACNNNNNGTC 1 cut(s) 553
EaeI YGGCCR 4 cut(s) 265, 416, 486, 490
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 3 cut(s) 176, 468, 705
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 3 cut(s) 176, 468, 705
EciI GGCGGA 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 4 cut(s) 8, 77, 287, 641
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 2 cut(s) 367, 800
Eco52I CGGCCG 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 75
Eco88I CYCGRG 4 cut(s) 449, 468, 571, 749
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 251, 413
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 4 cut(s) 8, 77, 287, 641
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 2 cut(s) 153, 473
FaeI CATG 5 cut(s) 37, 249, 440, 610, 676
FaiI YATR 5 cut(s) 35, 247, 438, 608, 674
FaqI GGGAC 1 cut(s) 813
FatI CATG 5 cut(s) 33, 245, 436, 606, 672
FauI CCCGC 5 cut(s) 118, 162, 240, 618, 679
FbaI TGATCA 3 cut(s) 46, 384, 564
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 6 cut(s) 361, 486, 528, 541, 544, 658
FokI GGATG 3 cut(s) 554, 689, 703
FriOI GRGCYC 4 cut(s) 8, 77, 287, 641
FseI GGCCGGCC 1 cut(s) 492
Fsp4HI GCNGC 6 cut(s) 361, 486, 528, 541, 544, 658
FspBI CTAG 1 cut(s) 426
GluI GCNGC 6 cut(s) 361, 486, 528, 541, 544, 658
GsuI CTGGAG 2 cut(s) 274, 653
HaeIII GGCC 6 cut(s) 251, 267, 418, 488, 492, 668
HapII CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
HgaI GACGC 1 cut(s) 565
Hin1I GRCGYC 2 cut(s) 123, 617
Hin1II CATG 5 cut(s) 37, 249, 440, 610, 676
HinfI GANTC 3 cut(s) 242, 551, 719
HpaII CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
HphI GGTGA 4 cut(s) 279, 379, 394, 574
Hpy166II GTNNAC 3 cut(s) 130, 367, 583
Hpy188I TCNGA 3 cut(s) 241, 400, 813
Hpy188III TCNNGA 5 cut(s) 34, 50, 426, 571, 700
Hpy8I GTNNAC 3 cut(s) 130, 367, 583
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 3 cut(s) 123, 617, 681
HpyCH4V TGCA 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 2 cut(s) 651, 810
HpySE526I ACGT 3 cut(s) 123, 617, 681
Hsp92I GRCGYC 2 cut(s) 123, 617
Hsp92II CATG 5 cut(s) 37, 249, 440, 610, 676
KroI GCCGGC 3 cut(s) 165, 418, 488
KroNI GCCGGC 3 cut(s) 167, 420, 490
Ksp22I TGATCA 3 cut(s) 46, 384, 564
Kzo9I GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
LmnI GCTCC 4 cut(s) 11, 80, 98, 104
MaeI CTAG 1 cut(s) 426
MaeII ACGT 3 cut(s) 123, 617, 681
MaeIII GTNAC 1 cut(s) 215
MalI GATC 8 cut(s) 48, 140, 386, 455, 534, 566, 735, 815
MbiI CCGCTC 1 cut(s) 657
MboI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
MboII GAAGA 4 cut(s) 10, 193, 485, 722
MflI RGATCY 2 cut(s) 532, 813
MhlI GDGCHC 6 cut(s) 8, 77, 287, 405, 641, 758
MlyI GAGTC 2 cut(s) 560, 728
MmeI TCCRAC 1 cut(s) 219
MroNI GCCGGC 3 cut(s) 165, 418, 488
MslI CAYNNNNRTG 2 cut(s) 380, 777
MspI CCGG 9 cut(s) 83, 96, 166, 419, 450, 469, 489, 497, 509
MspR9I CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
MvaI CCWGG 2 cut(s) 253, 415
MvnI CGCG 4 cut(s) 363, 527, 602, 625
MwoI GCNNNNNNNGC 1 cut(s) 438
NaeI GCCGGC 3 cut(s) 167, 420, 490
NciI CCSGG 6 cut(s) 83, 450, 451, 469, 470, 509
NcoI CCATGG 1 cut(s) 436
NdeII GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
NgoMIV GCCGGC 3 cut(s) 165, 418, 488
NlaIII CATG 5 cut(s) 37, 249, 440, 610, 676
NlaIV GGNNCC 7 cut(s) 7, 100, 369, 501, 667, 773, 801
OliI CACNNNNGTG 2 cut(s) 380, 777
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 775
PdiI GCCGGC 3 cut(s) 167, 420, 490
PfeI GAWTC 1 cut(s) 242
PflFI GACNNNGTC 2 cut(s) 125, 619
PkrI GCNGC 6 cut(s) 362, 487, 529, 542, 545, 659
PleI GAGTC 2 cut(s) 559, 727
PpsI GAGTC 2 cut(s) 559, 727
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 251, 413
PspGI CCWGG 2 cut(s) 251, 413
PspN4I GGNNCC 7 cut(s) 7, 100, 369, 501, 667, 773, 801
PspPI GGNCC 4 cut(s) 249, 367, 666, 800
PsuI RGATCY 2 cut(s) 532, 813
PsyI GACNNNGTC 2 cut(s) 125, 619
RigI GGCCGGCC 1 cut(s) 492
RsaI GTAC 2 cut(s) 177, 263
RsaNI GTAC 2 cut(s) 176, 262
RseI CAYNNNNRTG 2 cut(s) 380, 777
SacI GAGCTC 1 cut(s) 77
SatI GCNGC 6 cut(s) 361, 486, 528, 541, 544, 658
Sau3AI GATC 8 cut(s) 46, 138, 384, 453, 532, 564, 733, 813
Sau96I GGNCC 4 cut(s) 249, 367, 666, 800
SchI GAGTC 2 cut(s) 560, 728
ScrFI CCNGG 8 cut(s) 83, 253, 415, 450, 451, 469, 470, 509
SduI GDGCHC 6 cut(s) 8, 77, 287, 405, 641, 758
SinI GGWCC 2 cut(s) 367, 800
SmaI CCCGGG 2 cut(s) 451, 470
SmiMI CAYNNNNRTG 2 cut(s) 380, 777
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 8 cut(s) 81, 251, 413, 448, 449, 467, 468, 507
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 3 cut(s) 126, 620, 684
TatI WGTACW 1 cut(s) 175
TauI GCSGC 6 cut(s) 363, 488, 530, 543, 546, 660
TfiI GAWTC 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 22, 234
TspMI CCCGGG 2 cut(s) 449, 468
Tth111I GACNNNGTC 2 cut(s) 125, 619
VpaK11BI GGWCC 2 cut(s) 367, 800
XbaI TCTAGA 1 cut(s) 425
XmaI CCCGGG 2 cut(s) 449, 468
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 1 cut(s) 426
ZraI GACGTC 2 cut(s) 124, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.