FvH4_3g07540

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008773
Physical Location & Seq
Reverse (-)
4460161 .. 4465455
5295 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07540.t2

Sequence Viewer

Length: 1137 bp
ATGGCCTCAAGAAAGTTCGAAGGCATCGCGATGCTGTCGATGTACGACGACGAAGAAGAGGACGACGACATGGACGACGCTGAAGAGCACGGAAATCAAGAACACCGCGGCGGAGATAGAGACGACGGAGAACGGCGGGAACAACACGGCGACGCGTCCATGCTGGATGTGGATGCACCGGCGTCCGTTGATTCTGGAAACGACGATAATGCAACGAGCGGTGCGGATGATGAGAACTTGTCCCGGAGTGAACAGCAACTGTCTCAGGCGACGGGATCGCCGCAACGGGCGGTGGCGCCGGAGAAGAGGAGTAGGAGAGGGACGCTTGTGGACTACGGTCATGATGAAGTTGCTATGTCTCCTGAGCCTGAGGATGGTGAAATTGACGGAGGTGGAGGCATTTTGTCTGCTAATGGTGATTCACAGGATAAGACACCTCGGGCAACTCCTCAAATATCTGAGCCATCACAATCTGATGTGATGAATAAGACTGTGCTTGAATCAGATGGTAGAAATACTGCAGAGGCTGTTGCGGAGGATAAGAAAGATGTTGATCCATTAGATAATTTTCTTCCACCACCTTTAAAGATCAAGTGCTCAGAGGAGCTGCAAAGAAAGATTCAAAAGTTCCTTGAATTAAAGAGAGCAGGGAAAAGCTTCAATGAAGAAGTGCGGAAAAAGAAAGATTATAGGAACCCAGACTTTCTATTGCATGCAGTGCGGTATCAAGATATTGATCAGATAGGTTCATGCTTCAATAAAGATGTATTTGACCCTCATGGATTCGACAAAAGCGACTACTATGATGAAATAGAGGCTGATTCTAGACGTGAACTGGAGAGGATTAGTCAAGAAAAAAAGAATCGTCAGAATCTTGAATTTGTTTCTGGTGGAAGACAATCTACAGTAGTAGGTATAACCATGGCTGCTACTGGGTCAAACCCCTTACCACCTGCTGCTGATACCATCCCTCGAGACGGTAGACAGAATAAGAAGTCGAAGTGGGATAAGATAGATGGGACTCATGCAACATTATTATCTTCTGCTAATGCTGGTGGATACACTTTGGGGCATCAGAAGCGAAGAGAGGTGGAGGAGAAGAGATCTAGCGAAAGGAAGTTAGACAGAAGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

379

Amino Acids

42.09

Weight (kDa)

4.84

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 191 - 281 2.7e-28 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 961
Acc36I ACCTGC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 295
AccBSI CCGCTC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 982
AccII CGCG 3 cut(s) 29, 108, 155
AclWI GGATC 2 cut(s) 283, 548
AcsI RAATTY 1 cut(s) 878
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 2 cut(s) 182, 296
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 2 cut(s) 374, 977
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 6 cut(s) 500, 623, 635, 661, 757, 878
AjiI CACGTC 1 cut(s) 830
AluBI AGCT 2 cut(s) 607, 657
AluI AGCT 2 cut(s) 607, 657
Alw21I GWGCWC 2 cut(s) 90, 599
Alw26I GTCTC 4 cut(s) 114, 267, 363, 969
AlwI GGATC 2 cut(s) 283, 548
AlwNI CAGNNNCTG 2 cut(s) 259, 527
Ama87I CYCGRG 2 cut(s) 438, 972
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 607, 926, 956
ApoI RAATTY 1 cut(s) 878
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 389, 428
AsuII TTCGAA 1 cut(s) 18
AvaI CYCGRG 2 cut(s) 438, 972
AxyI CCTNAGG 1 cut(s) 369
BanI GGYRCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 886, 918
BbsI GAAGAC 1 cut(s) 901
Bbv12I GWGCWC 2 cut(s) 90, 599
BbvI GCAGC 3 cut(s) 594, 913, 943
BccI CCATC 5 cut(s) 368, 472, 500, 974, 1010
BceAI ACGGC 2 cut(s) 149, 163
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciVI GTATCC 1 cut(s) 1052
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 4 cut(s) 114, 267, 363, 969
BfaI CTAG 2 cut(s) 825, 1107
BfmI CTRYAG 2 cut(s) 519, 903
BfoI RGCGCY 1 cut(s) 299
BfuAI ACCTGC 1 cut(s) 961
BfuI GTATCC 1 cut(s) 1052
BglII AGATCT 1 cut(s) 1103
BisI GCNGC 5 cut(s) 109, 281, 608, 927, 957
BlsI GCNGC 5 cut(s) 110, 282, 609, 928, 958
Bme1390I CCNGG 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 438, 972
BmgBI CACGTC 1 cut(s) 830
BmiI GGNNCC 2 cut(s) 297, 695
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 4 cut(s) 21, 33, 163, 1081
BmuI ACTGGG 1 cut(s) 942
BoxI GACNNNNGTC 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 901
BpmI CTGGAG 1 cut(s) 857
Bpu10I CCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 244
BsaBI GATNNNNATC 2 cut(s) 552, 735
BsaHI GRCGYC 2 cut(s) 182, 296
BsaJI CCNNGG 3 cut(s) 106, 437, 921
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 977
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 840, 937
Bse21I CCTNAGG 1 cut(s) 369
Bse8I GATNNNNATC 2 cut(s) 552, 735
BseDI CCNNGG 3 cut(s) 106, 437, 921
BseGI GGATG 5 cut(s) 172, 178, 232, 379, 966
BseJI GATNNNNATC 2 cut(s) 552, 735
BseLI CCNNNNNNNGG 2 cut(s) 374, 977
BseMII CTCAG 5 cut(s) 278, 354, 360, 450, 612
BseNI ACTGG 2 cut(s) 840, 937
BseRI GAGGAG 4 cut(s) 322, 438, 617, 1109
BseXI GCAGC 3 cut(s) 594, 913, 943
Bsh1236I CGCG 3 cut(s) 29, 108, 155
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 90, 599
BsiHKCI CYCGRG 2 cut(s) 438, 972
BsiSI CCGG 3 cut(s) 179, 244, 299
BslFI GGGAC 3 cut(s) 226, 334, 1033
BslI CCNNNNNNNGG 2 cut(s) 374, 977
BsmAI GTCTC 4 cut(s) 114, 267, 363, 969
BsmBI CGTCTC 2 cut(s) 114, 969
BsmFI GGGAC 3 cut(s) 226, 334, 1033
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 2 cut(s) 438, 972
Bsp119I TTCGAA 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 90, 599
Bsp143I GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
Bsp19I CCATGG 1 cut(s) 921
Bsp68I TCGCGA 1 cut(s) 29
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 277, 355, 361, 451, 611
BspFNI CGCG 3 cut(s) 29, 108, 155
BspHI TCATGA 2 cut(s) 340, 1133
BspLI GGNNCC 2 cut(s) 297, 695
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 961
BspPI GGATC 2 cut(s) 283, 548
BspQI GCTCTTC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 18
BspT107I GGYRCC 1 cut(s) 295
BsrBI CCGCTC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 840, 937
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 106, 437, 921
BssMI GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
BssNI GRCGYC 2 cut(s) 182, 296
BssT1I CCWWGG 1 cut(s) 921
Bst4CI ACNGT 5 cut(s) 261, 338, 493, 907, 980
Bst6I CTCTTC 5 cut(s) 51, 78, 299, 1078, 1094
BstACI GRCGYC 2 cut(s) 182, 296
BstAPI GCANNNNNTGC 1 cut(s) 718
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 5 cut(s) 264, 363, 369, 459, 598
BstDSI CCRYGG 2 cut(s) 106, 921
BstF5I GGATG 5 cut(s) 172, 178, 232, 379, 966
BstFNI CGCG 3 cut(s) 29, 108, 155
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 6 cut(s) 278, 556, 591, 739, 1106, 1133
BstMAI GTCTC 4 cut(s) 114, 267, 363, 969
BstMBI GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
BstMWI GCNNNNNNNGC 2 cut(s) 718, 1078
BstNSI RCATGY 1 cut(s) 716
BstPAI GACNNNNGTC 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 2 cut(s) 519, 903
BstUI CGCG 3 cut(s) 29, 108, 155
BstV1I GCAGC 3 cut(s) 594, 913, 943
BstV2I GAAGAC 1 cut(s) 901
BstX2I RGATCY 1 cut(s) 1103
BstYI RGATCY 1 cut(s) 1103
Bsu36I CCTNAGG 1 cut(s) 369
BsuI GTATCC 1 cut(s) 1052
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 2 cut(s) 106, 921
BtgZI GCGATG 2 cut(s) 10, 44
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 5 cut(s) 172, 178, 232, 379, 966
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 723
BtuMI TCGCGA 1 cut(s) 29
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 1 cut(s) 714
CaiI CAGNNNCTG 2 cut(s) 259, 527
CciI TCATGA 2 cut(s) 340, 1133
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 178
Cfr42I CCGCGG 1 cut(s) 109
CseI GACGC 5 cut(s) 86, 144, 161, 171, 331
Csp6I GTAC 1 cut(s) 43
CviAII CATG 9 cut(s) 70, 160, 341, 713, 750, 779, 922, 1025, 1134
CviJI RGCY 8 cut(s) 5, 367, 463, 527, 607, 657, 818, 926
CviKI_1 RGCY 8 cut(s) 5, 367, 463, 527, 607, 657, 818, 926
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 5 cut(s) 264, 363, 369, 459, 598
DinI GGCGCC 1 cut(s) 297
DpnI GATC 6 cut(s) 277, 555, 590, 738, 1105, 1132
DpnII GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
DraI TTTAAA 1 cut(s) 585
Eam1104I CTCTTC 5 cut(s) 51, 78, 299, 1078, 1094
EarI CTCTTC 5 cut(s) 51, 78, 299, 1078, 1094
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 921
Eco57I CTGAAG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 369
Eco88I CYCGRG 2 cut(s) 438, 972
EcoT14I CCWWGG 1 cut(s) 921
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 921
Esp3I CGTCTC 2 cut(s) 114, 969
FaeI CATG 9 cut(s) 73, 163, 344, 716, 753, 782, 925, 1028, 1137
FaqI GGGAC 3 cut(s) 226, 334, 1033
FatI CATG 9 cut(s) 69, 159, 340, 712, 749, 778, 921, 1024, 1133
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 982
Fnu4HI GCNGC 5 cut(s) 109, 281, 608, 927, 957
FokI GGATG 5 cut(s) 179, 185, 239, 386, 953
Fsp4HI GCNGC 5 cut(s) 109, 281, 608, 927, 957
FspBI CTAG 2 cut(s) 825, 1107
GlaI GCGC 1 cut(s) 297
GluI GCNGC 5 cut(s) 109, 281, 608, 927, 957
GsuI CTGGAG 1 cut(s) 857
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 179, 244, 299
HgaI GACGC 5 cut(s) 86, 144, 161, 171, 331
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 2 cut(s) 182, 296
Hin1II CATG 9 cut(s) 73, 163, 344, 716, 753, 782, 925, 1028, 1137
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 9 cut(s) 191, 419, 500, 619, 783, 821, 862, 871, 1021
HpaII CCGG 3 cut(s) 179, 244, 299
HphI GGTGA 2 cut(s) 389, 428
Hpy166II GTNNAC 4 cut(s) 251, 331, 833, 983
Hpy188I TCNGA 7 cut(s) 460, 475, 505, 601, 741, 870, 1077
Hpy8I GTNNAC 4 cut(s) 251, 331, 833, 983
Hpy99I CGWCG 8 cut(s) 50, 53, 68, 80, 128, 155, 206, 274
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 5 cut(s) 261, 338, 493, 907, 980
HpyCH4IV ACGT 1 cut(s) 829
HpyCH4V TGCA 7 cut(s) 176, 212, 521, 610, 712, 716, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 718, 1078
HpyF3I CTNAG 5 cut(s) 264, 363, 369, 459, 598
HpySE526I ACGT 1 cut(s) 829
Hsp92I GRCGYC 2 cut(s) 182, 296
Hsp92II CATG 9 cut(s) 73, 163, 344, 716, 753, 782, 925, 1028, 1137
HspAI GCGC 1 cut(s) 296
KasI GGCGCC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 736
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
LguI GCTCTTC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 3 cut(s) 594, 913, 943
LweI GCATC 4 cut(s) 21, 33, 163, 1081
MaeI CTAG 2 cut(s) 825, 1107
MaeII ACGT 1 cut(s) 829
MalI GATC 6 cut(s) 277, 555, 590, 738, 1105, 1132
MbiI CCGCTC 1 cut(s) 219
MboI GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
MflI RGATCY 1 cut(s) 1103
MhlI GDGCHC 2 cut(s) 90, 599
MluCI AATT 4 cut(s) 381, 565, 635, 878
MluI ACGCGT 1 cut(s) 153
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 1015
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 2 cut(s) 584, 638
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 3 cut(s) 179, 244, 299
MspR9I CCNGG 1 cut(s) 244
MvnI CGCG 3 cut(s) 29, 108, 155
MwoI GCNNNNNNNGC 2 cut(s) 718, 1078
NarI GGCGCC 1 cut(s) 296
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 921
NdeII GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
NlaIII CATG 9 cut(s) 73, 163, 344, 716, 753, 782, 925, 1028, 1137
NlaIV GGNNCC 2 cut(s) 297, 695
NruI TCGCGA 1 cut(s) 29
NspI RCATGY 1 cut(s) 716
NspV TTCGAA 1 cut(s) 18
PaeI GCATGC 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 972
PagI TCATGA 2 cut(s) 340, 1133
PaqCI CACCTGC 1 cut(s) 961
PciSI GCTCTTC 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 792
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 8 cut(s) 191, 419, 500, 619, 783, 821, 862, 871
PfoI TCCNGGA 1 cut(s) 242
PkrI GCNGC 5 cut(s) 110, 282, 609, 928, 958
PleI GAGTC 1 cut(s) 1015
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 1015
PshAI GACNNNNGTC 1 cut(s) 336
PspN4I GGNNCC 2 cut(s) 297, 695
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 2 cut(s) 259, 527
PsuI RGATCY 1 cut(s) 1103
RruI TCGCGA 1 cut(s) 29
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 29
SacII CCGCGG 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 2 cut(s) 584, 638
SatI GCNGC 5 cut(s) 109, 281, 608, 927, 957
Sau3AI GATC 6 cut(s) 275, 553, 588, 736, 1103, 1130
SchI GAGTC 1 cut(s) 1015
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 2 cut(s) 90, 599
SfaNI GCATC 4 cut(s) 21, 33, 163, 1081
SfcI CTRYAG 2 cut(s) 519, 903
SfoI GGCGCC 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 972
Sfr303I CCGCGG 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 18
SgrAI CRCCGGYG 1 cut(s) 178
SgrBI CCGCGG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 972
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 7, 972
SmoI CTYRAG 2 cut(s) 7, 972
SphI GCATGC 1 cut(s) 716
Sse9I AATT 4 cut(s) 381, 565, 635, 878
SspDI GGCGCC 1 cut(s) 295
SspMI CTAG 2 cut(s) 825, 1107
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 5 cut(s) 261, 338, 493, 907, 980
TaiI ACGT 1 cut(s) 832
TaqI TCGA 5 cut(s) 18, 38, 786, 973, 998
TasI AATT 4 cut(s) 381, 565, 635, 878
TauI GCSGC 2 cut(s) 111, 283
TfiI GAWTC 8 cut(s) 191, 419, 500, 619, 783, 821, 862, 871
Tru1I TTAA 2 cut(s) 584, 638
Tru9I TTAA 2 cut(s) 584, 638
TscAI CASTG 1 cut(s) 723
TseI GCWGC 3 cut(s) 607, 926, 956
TspDTI ATGAA 5 cut(s) 360, 497, 678, 738, 822
TspGWI ACGGA 4 cut(s) 105, 141, 175, 402
TspRI CASTG 1 cut(s) 723
XapI RAATTY 1 cut(s) 878
XbaI TCTAGA 1 cut(s) 824
XceI RCATGY 1 cut(s) 716
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XhoI CTCGAG 1 cut(s) 972
XmiI GTMKAC 1 cut(s) 982
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 2 cut(s) 825, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.