Rh5BG082700

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008773
Physical Location & Seq
Forward (+)
7106727 .. 7110753
4027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG082700.1

Sequence Viewer

Length: 1218 bp
ATGGCCTCGAGAAAGCGGCAATTCGAGGGCATCGCGATGCTGTCGATGTACAACGACGAGGAAGAGGAAGAAGAAGAAGAGGACGAAGACATGGACGACGCTGAAGAACGCGGCGAACAAGAAGAGCGCGGCGGAGAGAGAGAAGACGGAGAACGGCGGGAACACGGCGGCGATGCCTCCATGTTGGATGTCGATGCGACGGCGGCCGTTGATTCTGGTAACGATGACAATGCAACGAGCGGTGTGGATGATGAGAACTCGGCGCGGAGTGAAGGGCAGTTTCGTCGTTCTTCGTCGCAACAGCAGCTCTCTCAGGCGACGGGATCGCCGCAGCAAGTGGCGCCGGGGAAGTTGAGTAGGAGAGGGACGCTTGTGGACTATGGCCATGATGAAGTTGCCATGTCTCCAGAGCCTGAGGAGGGTGAAATTGATGGAAGTGGTCGGATTTTGTCTACCAATGGTGATTCACATGATAAGACACCTCAGTTAAGCAATCAAGTAACTCCTCAAATATCTGAGCCATCACAATCTGAAACACTGAATGAGACTATACTTGAATCAGAGGGTAGAAATGCTACAGAGGCTGTTGCAGAAGATGAAAAAAATGTTGATCCATTAGATAAGTTTCTTCCACCACCATTAAAGCTCAAGTGCTCAGAGGAGCTGCAAAGAAAGATTCAAAAGTTTCTTGAATTAAAGAGAGCTGGGAAAAGCTTCAATGAAGAAGTGCGGAAAAAGAAGGACTATAGGAATCCAGACTTTCTATTGCATGCAGTGCGGTATCAAGATATTGATCAGATAGGTTCATGCTTCAGTAAAGATGTATTCGACCCTCATGGATTTGACAAAAGCGACTACTATGATGAAATAGAGGCTGATTCGAGGCGTGAAATGGAAAGGGTTAATCAGGAAAAAAAGAACCGTCAGAATCTTGAATTTGTTTCTGGTGGAAGACAATCTACAATAGTTGGTGCTGCACCTAAACTTAATGTACCTCTTCCAGGTATAACTTCTGGGTTGAGTTCCTTACCGGCTGCTGCTGATATCATCCCTCGGGACGGTAGACAGAATAAGAAGTCGAAGTGGGATAAGATTGATGGGACTCATGCAACACTCTTATCTTCTGCTAATGCTGGTGGATACAGTTTGGGGCAGCAGAAACGGAGAGATGTGGAGGAGAAGAGATCTAGTGAAAGGAAGTTAGACAGAAGATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

405

Amino Acids

45.2

Weight (kDa)

4.75

Isoelectric Point (pI)

64.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 210 - 302 3.4e-29 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 340
AccBSI CCGCTC 1 cut(s) 240
AccI GTMKAC 2 cut(s) 452, 1063
AccII CGCG 4 cut(s) 35, 111, 129, 265
AclWI GGATC 2 cut(s) 331, 605
AcoI YGGCCR 2 cut(s) 204, 382
AcsI RAATTY 1 cut(s) 935
AcuI CTGAAG 2 cut(s) 123, 796
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 2 cut(s) 50, 993
AfiI CCNNNNNNNGG 3 cut(s) 419, 1001, 1058
AgsI TTSAA 5 cut(s) 557, 680, 692, 718, 935
AjnI CCWGG 1 cut(s) 1000
AluBI AGCT 5 cut(s) 307, 646, 664, 704, 714
AluI AGCT 5 cut(s) 307, 646, 664, 704, 714
Alw21I GWGCWC 1 cut(s) 656
Alw26I GTCTC 2 cut(s) 408, 539
AlwI GGATC 2 cut(s) 331, 605
AlwNI CAGNNNCTG 2 cut(s) 413, 584
Ama87I CYCGRG 2 cut(s) 7, 1053
AoxI GGCC 3 cut(s) 3, 204, 382
ApeKI GCWGC 7 cut(s) 304, 331, 664, 974, 1034, 1037, 1153
ApoI RAATTY 1 cut(s) 935
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 3 cut(s) 129, 265, 343
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 434, 473
AvaI CYCGRG 2 cut(s) 7, 1053
AxyI CCTNAGG 1 cut(s) 414
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 1 cut(s) 340
BarI GAAGNNNNNNTAC 2 cut(s) 943, 975
BbsI GAAGAC 3 cut(s) 93, 150, 958
Bbv12I GWGCWC 1 cut(s) 656
BbvI GCAGC 7 cut(s) 316, 343, 651, 961, 1021, 1024, 1165
BccI CCATC 3 cut(s) 425, 529, 1091
BceAI ACGGC 4 cut(s) 170, 181, 191, 216
BcgI CGANNNNNNTGC 4 cut(s) 212, 246, 266, 300
BciT130I CCWGG 1 cut(s) 1002
BciVI GTATCC 1 cut(s) 1133
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 408, 539
BfaI CTAG 1 cut(s) 1188
BfmI CTRYAG 2 cut(s) 576, 745
BfoI RGCGCY 1 cut(s) 344
BfuI GTATCC 1 cut(s) 1133
BglII AGATCT 1 cut(s) 1184
Bme1390I CCNGG 2 cut(s) 345, 1002
BmeT110I CYCGRG 2 cut(s) 7, 1053
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 2 cut(s) 345, 1002
BmsI GCATC 4 cut(s) 27, 39, 163, 184
BpiI GAAGAC 3 cut(s) 93, 150, 958
BpmI CTGGAG 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 341
BsaJI CCNNGG 2 cut(s) 344, 1052
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 1001, 1058
Bse118I RCCGGY 1 cut(s) 1030
Bse21I CCTNAGG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 2 cut(s) 344, 1052
BseGI GGATG 3 cut(s) 193, 253, 1047
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 419, 1001, 1058
BseMII CTCAG 5 cut(s) 326, 405, 497, 507, 669
BseRI GAGGAG 4 cut(s) 431, 495, 674, 1190
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 7 cut(s) 316, 343, 651, 961, 1021, 1024, 1165
BseYI CCCAGC 1 cut(s) 704
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 4 cut(s) 35, 111, 129, 265
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 3 cut(s) 5, 206, 384
BshNI GGYRCC 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 656
BsiHKCI CYCGRG 2 cut(s) 7, 1053
BsiSI CCGG 2 cut(s) 344, 1031
BslFI GGGAC 3 cut(s) 379, 1070, 1114
BslI CCNNNNNNNGG 3 cut(s) 419, 1001, 1058
BsmAI GTCTC 2 cut(s) 408, 539
BsmFI GGGAC 3 cut(s) 379, 1070, 1114
BsnI GGCC 3 cut(s) 5, 206, 384
BsoBI CYCGRG 2 cut(s) 7, 1053
Bsp1286I GDGCHC 1 cut(s) 656
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 5 cut(s) 323, 610, 793, 1184, 1211
Bsp68I TCGCGA 1 cut(s) 35
BspANI GGCC 3 cut(s) 5, 206, 384
BspCNI CTCAG 5 cut(s) 325, 406, 496, 508, 668
BspFNI CGCG 4 cut(s) 35, 111, 129, 265
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 2 cut(s) 331, 605
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 340
BsrBI CCGCTC 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 1030
BsrGI TGTACA 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 1030
BssECI CCNNGG 2 cut(s) 344, 1052
BssMI GATC 5 cut(s) 323, 610, 793, 1184, 1211
BssNI GRCGYC 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 3 cut(s) 923, 1061, 1145
Bst6I CTCTTC 5 cut(s) 57, 72, 117, 1002, 1175
BstACI GRCGYC 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 775
BstAUI TGTACA 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 5 cut(s) 312, 414, 483, 516, 655
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 3 cut(s) 193, 253, 1047
BstFNI CGCG 4 cut(s) 35, 111, 129, 265
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 3 cut(s) 129, 265, 343
BstKTI GATC 5 cut(s) 326, 613, 796, 1187, 1214
BstMAI GTCTC 2 cut(s) 408, 539
BstMBI GATC 5 cut(s) 323, 610, 793, 1184, 1211
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 5 cut(s) 203, 304, 340, 581, 775
BstNI CCWGG 1 cut(s) 1002
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 2 cut(s) 343, 1000
BstSFI CTRYAG 2 cut(s) 576, 745
BstUI CGCG 4 cut(s) 35, 111, 129, 265
BstV1I GCAGC 7 cut(s) 316, 343, 651, 961, 1021, 1024, 1165
BstV2I GAAGAC 3 cut(s) 93, 150, 958
BstX2I RGATCY 1 cut(s) 1184
BstYI RGATCY 1 cut(s) 1184
BstZI CGGCCG 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 414
BsuI GTATCC 1 cut(s) 1133
BsuRI GGCC 3 cut(s) 5, 206, 384
BtgZI GCGATG 3 cut(s) 16, 50, 186
BtsCI GGATG 3 cut(s) 193, 253, 1047
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 536, 780
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 771
CaiI CAGNNNCTG 2 cut(s) 413, 584
CfoI GCGC 3 cut(s) 129, 265, 343
Cfr10I RCCGGY 1 cut(s) 1030
CseI GACGC 2 cut(s) 107, 376
Csp6I GTAC 2 cut(s) 49, 992
CviAII CATG 9 cut(s) 91, 181, 386, 400, 470, 770, 807, 836, 1106
CviQI GTAC 2 cut(s) 49, 992
DdeI CTNAG 5 cut(s) 312, 414, 483, 516, 655
DinI GGCGCC 1 cut(s) 342
DpnI GATC 5 cut(s) 325, 612, 795, 1186, 1213
DpnII GATC 5 cut(s) 323, 610, 793, 1184, 1211
EaeI YGGCCR 2 cut(s) 204, 382
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 5 cut(s) 57, 72, 117, 1002, 1175
EarI CTCTTC 5 cut(s) 57, 72, 117, 1002, 1175
EciI GGCGGA 1 cut(s) 147
EclXI CGGCCG 1 cut(s) 204
Eco32I GATATC 1 cut(s) 1045
Eco52I CGGCCG 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 123, 796
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 2 cut(s) 7, 1053
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 1000
EcoRV GATATC 1 cut(s) 1045
EgeI GGCGCC 1 cut(s) 342
EheI GGCGCC 1 cut(s) 342
FaeI CATG 9 cut(s) 94, 184, 389, 403, 473, 773, 810, 839, 1109
FaqI GGGAC 3 cut(s) 379, 1070, 1114
FatI CATG 9 cut(s) 90, 180, 385, 399, 469, 769, 806, 835, 1105
FauI CCCGC 1 cut(s) 150
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 2 cut(s) 452, 1063
FokI GGATG 3 cut(s) 200, 260, 1034
FspBI CTAG 1 cut(s) 1188
GlaI GCGC 3 cut(s) 128, 264, 342
GsaI CCCAGC 1 cut(s) 708
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 3 cut(s) 5, 206, 384
HapII CCGG 2 cut(s) 344, 1031
HgaI GACGC 2 cut(s) 107, 376
HhaI GCGC 3 cut(s) 129, 265, 343
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 9 cut(s) 94, 184, 389, 403, 473, 773, 810, 839, 1109
Hin6I GCGC 3 cut(s) 127, 263, 341
HinP1I GCGC 3 cut(s) 127, 263, 341
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 8 cut(s) 212, 464, 557, 676, 751, 878, 928, 1102
HpaII CCGG 2 cut(s) 344, 1031
HphI GGTGA 2 cut(s) 434, 473
Hpy166II GTNNAC 3 cut(s) 376, 453, 1064
Hpy188I TCNGA 7 cut(s) 444, 517, 532, 562, 658, 798, 927
Hpy8I GTNNAC 3 cut(s) 376, 453, 1064
Hpy99I CGWCG 6 cut(s) 59, 101, 202, 288, 298, 322
HpyAV CCTTC 2 cut(s) 266, 733
HpyCH4III ACNGT 3 cut(s) 923, 1061, 1145
HpyCH4V TGCA 7 cut(s) 233, 590, 667, 769, 773, 977, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 304, 340, 581, 775
HpyF3I CTNAG 5 cut(s) 312, 414, 483, 516, 655
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 9 cut(s) 94, 184, 389, 403, 473, 773, 810, 839, 1109
HspAI GCGC 3 cut(s) 127, 263, 341
KasI GGCGCC 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 5 cut(s) 323, 610, 793, 1184, 1211
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 7 cut(s) 316, 343, 651, 961, 1021, 1024, 1165
LweI GCATC 4 cut(s) 27, 39, 163, 184
MaeI CTAG 1 cut(s) 1188
MaeIII GTNAC 2 cut(s) 218, 499
MalI GATC 5 cut(s) 325, 612, 795, 1186, 1213
MbiI CCGCTC 1 cut(s) 240
MboI GATC 5 cut(s) 323, 610, 793, 1184, 1211
MflI RGATCY 1 cut(s) 1184
MhlI GDGCHC 1 cut(s) 656
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 4 cut(s) 20, 426, 692, 935
MluNI TGGCCA 1 cut(s) 384
Mly113I GGCGCC 1 cut(s) 341
MlyI GAGTC 1 cut(s) 1096
MmeI TCCRAC 2 cut(s) 165, 422
Mox20I TGGCCA 1 cut(s) 384
MroXI GAANNNNTTC 1 cut(s) 713
MscI TGGCCA 1 cut(s) 384
MseI TTAA 5 cut(s) 488, 641, 695, 903, 987
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 384
MspI CCGG 2 cut(s) 344, 1031
MspR9I CCNGG 2 cut(s) 345, 1002
MvaI CCWGG 1 cut(s) 1002
MvnI CGCG 4 cut(s) 35, 111, 129, 265
MwoI GCNNNNNNNGC 5 cut(s) 203, 304, 340, 581, 775
NarI GGCGCC 1 cut(s) 341
NciI CCSGG 1 cut(s) 345
NdeII GATC 5 cut(s) 323, 610, 793, 1184, 1211
NlaIII CATG 9 cut(s) 94, 184, 389, 403, 473, 773, 810, 839, 1109
NlaIV GGNNCC 1 cut(s) 342
NmeAIII GCCGAG 1 cut(s) 239
NruI TCGCGA 1 cut(s) 35
NspI RCATGY 1 cut(s) 773
PaeI GCATGC 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 7 cut(s) 212, 464, 557, 676, 751, 878, 928
PleI GAGTC 1 cut(s) 1096
PluTI GGCGCC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 1096
Psp6I CCWGG 1 cut(s) 1000
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 1 cut(s) 1000
PspN4I GGNNCC 1 cut(s) 342
PstNI CAGNNNCTG 2 cut(s) 413, 584
PsuI RGATCY 1 cut(s) 1184
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 2 cut(s) 50, 993
RsaNI GTAC 2 cut(s) 49, 992
RseI CAYNNNNRTG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 5 cut(s) 488, 641, 695, 903, 987
Sau3AI GATC 5 cut(s) 323, 610, 793, 1184, 1211
SchI GAGTC 1 cut(s) 1096
ScrFI CCNGG 2 cut(s) 345, 1002
SduI GDGCHC 1 cut(s) 656
SfaNI GCATC 4 cut(s) 27, 39, 163, 184
SfcI CTRYAG 2 cut(s) 576, 745
SfoI GGCGCC 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 7, 647
SmoI CTYRAG 2 cut(s) 7, 647
SphI GCATGC 1 cut(s) 773
Sse9I AATT 4 cut(s) 20, 426, 692, 935
SspDI GGCGCC 1 cut(s) 340
SspMI CTAG 1 cut(s) 1188
StyD4I CCNGG 2 cut(s) 343, 1000
TaaI ACNGT 3 cut(s) 923, 1061, 1145
TaqI TCGA 7 cut(s) 8, 24, 44, 192, 828, 881, 1079
TasI AATT 4 cut(s) 20, 426, 692, 935
TatI WGTACW 1 cut(s) 48
TauI GCSGC 6 cut(s) 19, 114, 132, 171, 206, 331
TfiI GAWTC 7 cut(s) 212, 464, 557, 676, 751, 878, 928
Tru1I TTAA 5 cut(s) 488, 641, 695, 903, 987
Tru9I TTAA 5 cut(s) 488, 641, 695, 903, 987
TscAI CASTG 2 cut(s) 543, 780
TseI GCWGC 7 cut(s) 304, 331, 664, 974, 1034, 1037, 1153
TspDTI ATGAA 5 cut(s) 405, 612, 735, 795, 879
TspGWI ACGGA 2 cut(s) 162, 1177
TspRI CASTG 2 cut(s) 543, 780
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 1 cut(s) 935
XceI RCATGY 1 cut(s) 773
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 452, 1063
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.