MD10G1277900.v1.1

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008773
Physical Location & Seq
Reverse (-)
36864393 .. 36868528
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1277900.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGCCTCGAGGAAGAAGCAATCCGACGCCATAGCTCTCCTGCTGTCCAACTACAACGACGAGGAAGAGGATGAAGATATGGAGGACGTTGAGCAACAAAACGACATAGAAGAAGAACTCGAAGGGGATGAAGAGCGACGAGAACGGAGAGAATACGACGATTACGGTGAGCTGAGAAAAGGCTCGGGAGATGCTGCAATGGTGGATGTGGATGGAACGGTGGCTGGTGAGTCTGGGAACGACGATGGCGCACCGCCGAATGCCGGGGATAATGTGGCGCCTTCGGATGGTTTGAATGGGAGAAGAAGAAGAGGGACGTTTACGATTGTGGACTATGGGCGTGATGAAGTTGCTATGTCTCCTGAGACTGAGGAGGGTGAAATAGAAGGAAGTCGTCGTGTTCCTGGTGATTTCCATGATAAGACACCTCCAGGAACAGTTCGCACTCCTCAGTTATCTGAACCGTCAAACTCTGACACAGTGAACGAAACTGTACTTGAATCAGAGGGTAGAGATGCCGCAGAAGCTGTTGCAGAAGAGCAAAAGGATATTGATCCATTAGATAAGTTTCTCCCGCCACCTTTAAAAGCGAAGTGTTCGGAGGAGCTGCAAAGGAAGATAAATAAGTTTCTTGAATACAAGAGAGCCGGGAAAAGCTTCAATGCAGAAGTGCGTAATAAGAAGGACTATCGGAATCCAAACTTTCTAGTGCATGCGGTGAGGTATCAAGATATTGATCAGATAGGGTCTTGCTTCAGTAAAGATGTATTTGACCCTCATGGATATGACAAAAGTGACTACTGTGATGAAATAGAGGCTGATATGAGGCGCGAAATGGAAAGGAAAGAGCAAGACAGGAAGAAGAGTCAAAAGGTTGAATATGTTTCAGGAGGAACACAGCCTGGAATAGTTGGTGCCGCACCTAAAATTAACGTACCTTTTACAGGTGTTTCAACCATGGCTGCTACTGGGTTGAACTCCTTACCTCCTGCAGCTGATGCCATCCCTCGGGAGGGTAGACAGAATAAGAAGTCAAAATGGGATAAGGTAGATGGTGATCGAAAAAATCCTCTTCCCTCAGGATTGCAGGATTCTATGTCTACCGTGGGGACTCATGCATCACTCCTATCTTCTGCTAGTGCTGGTGCTGGATACATGGCTTTTGCGCAGCAGAGACGGAGAGAGGTGGAGGAGAAACGATCTAGTGAAAGGAAGTTAGACAGAAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

411

Amino Acids

45.59

Weight (kDa)

4.82

Isoelectric Point (pI)

62.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 191 - 284 6.2e-29 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 7
Acc16I TGCGCA 1 cut(s) 1167
AccB1I GGYRCC 2 cut(s) 277, 914
AccI GTMKAC 2 cut(s) 1018, 1100
AccII CGCG 1 cut(s) 831
AciI CCGC 5 cut(s) 254, 519, 575, 716, 918
AclWI GGATC 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 739
AcyI GRCGYC 2 cut(s) 27, 278
AfaI GTAC 2 cut(s) 495, 936
AfiI CCNNNNNNNGG 6 cut(s) 263, 287, 944, 1008, 1012, 1013
AgsI TTSAA 7 cut(s) 295, 500, 635, 661, 878, 954, 976
AjnI CCWGG 3 cut(s) 403, 430, 901
AluBI AGCT 6 cut(s) 35, 172, 527, 607, 657, 995
AluI AGCT 6 cut(s) 35, 172, 527, 607, 657, 995
Alw26I GTCTC 3 cut(s) 359, 363, 1168
AlwI GGATC 1 cut(s) 548
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 3 cut(s) 7, 184, 1008
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 5 cut(s) 194, 607, 962, 992, 1168
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 4 cut(s) 251, 280, 831, 1168
AsuC2I CCSGG 2 cut(s) 265, 649
AsuHPI GGTGA 6 cut(s) 179, 239, 389, 419, 730, 1067
AvaI CYCGRG 3 cut(s) 7, 184, 1008
AxyI CCTNAGG 1 cut(s) 1078
BanI GGYRCC 2 cut(s) 277, 914
BbvI GCAGC 5 cut(s) 181, 594, 949, 1004, 1180
BccI CCATC 5 cut(s) 206, 239, 281, 1010, 1046
BciT130I CCWGG 3 cut(s) 405, 432, 903
BciVI GTATCC 1 cut(s) 1145
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 2 cut(s) 265, 649
BcoDI GTCTC 3 cut(s) 359, 363, 1168
BfaI CTAG 3 cut(s) 707, 1137, 1203
BfmI CTRYAG 1 cut(s) 990
BfoI RGCGCY 1 cut(s) 281
BfuI GTATCC 1 cut(s) 1145
BglII AGATCT 1 cut(s) 1226
BisI GCNGC 7 cut(s) 195, 519, 608, 918, 963, 993, 1169
BlsI GCNGC 7 cut(s) 196, 520, 609, 919, 964, 994, 1170
Bme1390I CCNGG 5 cut(s) 265, 405, 432, 649, 903
BmeT110I CYCGRG 3 cut(s) 7, 184, 1008
BmiI GGNNCC 2 cut(s) 279, 916
BmrFI CCNGG 5 cut(s) 265, 405, 432, 649, 903
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 4 cut(s) 181, 505, 988, 1127
BmuI ACTGGG 1 cut(s) 978
BpmI CTGGAG 1 cut(s) 414
BpuMI CCSGG 2 cut(s) 265, 649
BsaBI GATNNNNATC 3 cut(s) 552, 735, 1056
BsaHI GRCGYC 2 cut(s) 27, 278
BsaJI CCNNGG 4 cut(s) 264, 957, 1007, 1104
Bsc4I CCNNNNNNNGG 6 cut(s) 263, 287, 944, 1008, 1012, 1013
Bse1I ACTGG 1 cut(s) 973
Bse21I CCTNAGG 1 cut(s) 1078
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 3 cut(s) 552, 735, 1056
BseBI CCWGG 3 cut(s) 405, 432, 903
BseDI CCNNGG 4 cut(s) 264, 957, 1007, 1104
BseGI GGATG 6 cut(s) 76, 133, 211, 217, 292, 1002
BseJI GATNNNNATC 3 cut(s) 552, 735, 1056
BseLI CCNNNNNNNGG 6 cut(s) 263, 287, 944, 1008, 1012, 1013
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 5 cut(s) 164, 354, 360, 464, 1092
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 4 cut(s) 386, 438, 617, 1205
BseXI GCAGC 5 cut(s) 181, 594, 949, 1004, 1180
Bsh1236I CGCG 1 cut(s) 831
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 277, 914
BsiHKCI CYCGRG 3 cut(s) 7, 184, 1008
BsiSI CCGG 2 cut(s) 264, 648
BslFI GGGAC 2 cut(s) 328, 1123
BslI CCNNNNNNNGG 6 cut(s) 263, 287, 944, 1008, 1012, 1013
BsmAI GTCTC 3 cut(s) 359, 363, 1168
BsmBI CGTCTC 1 cut(s) 1168
BsmFI GGGAC 2 cut(s) 328, 1123
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 3 cut(s) 7, 184, 1008
Bsp143I GATC 5 cut(s) 553, 736, 1057, 1199, 1226
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 5 cut(s) 254, 519, 575, 716, 918
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 165, 355, 361, 463, 1091
BspFNI CGCG 1 cut(s) 831
BspLI GGNNCC 2 cut(s) 279, 916
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 1 cut(s) 548
BspQI GCTCTTC 2 cut(s) 126, 531
BspT107I GGYRCC 2 cut(s) 277, 914
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 4 cut(s) 264, 957, 1007, 1104
BssMI GATC 5 cut(s) 553, 736, 1057, 1199, 1226
BssNI GRCGYC 2 cut(s) 27, 278
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 3 cut(s) 405, 432, 903
Bst4CI ACNGT 8 cut(s) 167, 220, 439, 465, 481, 493, 803, 1105
Bst6I CTCTTC 6 cut(s) 60, 126, 304, 531, 857, 1077
BstACI GRCGYC 2 cut(s) 27, 278
BstAPI GCANNNNNTGC 1 cut(s) 998
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 5 cut(s) 173, 363, 369, 450, 1078
BstDSI CCRYGG 2 cut(s) 957, 1104
BstENI CCTNNNNNAGG 1 cut(s) 942
BstF5I GGATG 6 cut(s) 76, 133, 211, 217, 292, 1002
BstFNI CGCG 1 cut(s) 831
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 4 cut(s) 251, 280, 831, 1168
BstKTI GATC 5 cut(s) 556, 739, 1060, 1202, 1229
BstMAI GTCTC 3 cut(s) 359, 363, 1168
BstMBI GATC 5 cut(s) 553, 736, 1057, 1199, 1226
BstMWI GCNNNNNNNGC 2 cut(s) 524, 998
BstNI CCWGG 3 cut(s) 405, 432, 903
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 5 cut(s) 263, 403, 430, 647, 901
BstSFI CTRYAG 1 cut(s) 990
BstUI CGCG 1 cut(s) 831
BstV1I GCAGC 5 cut(s) 181, 594, 949, 1004, 1180
BstX2I RGATCY 1 cut(s) 1226
BstYI RGATCY 1 cut(s) 1226
Bsu36I CCTNAGG 1 cut(s) 1078
BsuI GTATCC 1 cut(s) 1145
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 2 cut(s) 957, 1104
BtsCI GGATG 6 cut(s) 76, 133, 211, 217, 292, 1002
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 714
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 4 cut(s) 251, 280, 831, 1168
CseI GACGC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 494, 935
CviAII CATG 6 cut(s) 416, 713, 779, 958, 1115, 1156
CviQI GTAC 2 cut(s) 494, 935
DdeI CTNAG 5 cut(s) 173, 363, 369, 450, 1078
DinI GGCGCC 1 cut(s) 279
DpnI GATC 5 cut(s) 555, 738, 1059, 1201, 1228
DpnII GATC 5 cut(s) 553, 736, 1057, 1199, 1226
DraI TTTAAA 1 cut(s) 585
Eam1104I CTCTTC 6 cut(s) 60, 126, 304, 531, 857, 1077
EarI CTCTTC 6 cut(s) 60, 126, 304, 531, 857, 1077
Eco130I CCWWGG 1 cut(s) 957
Eco57I CTGAAG 1 cut(s) 739
Eco81I CCTNAGG 1 cut(s) 1078
Eco88I CYCGRG 3 cut(s) 7, 184, 1008
EcoNI CCTNNNNNAGG 1 cut(s) 942
EcoRII CCWGG 3 cut(s) 403, 430, 901
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 1120
EgeI GGCGCC 1 cut(s) 279
EheI GGCGCC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 957
Esp3I CGTCTC 1 cut(s) 1168
FaeI CATG 6 cut(s) 419, 716, 782, 961, 1118, 1159
FaqI GGGAC 2 cut(s) 328, 1123
FatI CATG 6 cut(s) 415, 712, 778, 957, 1114, 1155
FauI CCCGC 1 cut(s) 582
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 2 cut(s) 1018, 1100
Fnu4HI GCNGC 7 cut(s) 195, 519, 608, 918, 963, 993, 1169
FokI GGATG 6 cut(s) 83, 140, 218, 224, 299, 989
Fsp4HI GCNGC 7 cut(s) 195, 519, 608, 918, 963, 993, 1169
FspBI CTAG 3 cut(s) 707, 1137, 1203
FspI TGCGCA 1 cut(s) 1167
GlaI GCGC 4 cut(s) 250, 279, 830, 1167
GluI GCNGC 7 cut(s) 195, 519, 608, 918, 963, 993, 1169
GsuI CTGGAG 1 cut(s) 414
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 264, 648
HgaI GACGC 1 cut(s) 35
HhaI GCGC 4 cut(s) 251, 280, 831, 1168
Hin1I GRCGYC 2 cut(s) 27, 278
Hin1II CATG 6 cut(s) 419, 716, 782, 961, 1118, 1159
Hin6I GCGC 4 cut(s) 249, 278, 829, 1166
HinP1I GCGC 4 cut(s) 249, 278, 829, 1166
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 6 cut(s) 230, 500, 694, 865, 1091, 1111
HpaII CCGG 2 cut(s) 264, 648
HphI GGTGA 6 cut(s) 179, 239, 389, 419, 730, 1067
Hpy166II GTNNAC 5 cut(s) 321, 331, 484, 1019, 1101
Hpy188I TCNGA 8 cut(s) 25, 286, 460, 475, 505, 601, 693, 741
Hpy188III TCNNGA 8 cut(s) 186, 362, 632, 728, 888, 1010, 1080, 1230
Hpy8I GTNNAC 5 cut(s) 321, 331, 484, 1019, 1101
Hpy99I CGWCG 6 cut(s) 29, 62, 141, 161, 245, 399
HpyAV CCTTC 4 cut(s) 116, 291, 380, 676
HpyCH4III ACNGT 8 cut(s) 167, 220, 439, 465, 481, 493, 803, 1105
HpyCH4IV ACGT 3 cut(s) 87, 317, 933
HpyCH4V TGCA 8 cut(s) 197, 533, 610, 665, 712, 992, 1087, 1118
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 998
HpyF3I CTNAG 5 cut(s) 173, 363, 369, 450, 1078
HpySE526I ACGT 3 cut(s) 87, 317, 933
Hsp92I GRCGYC 2 cut(s) 27, 278
Hsp92II CATG 6 cut(s) 419, 716, 782, 961, 1118, 1159
HspAI GCGC 4 cut(s) 249, 278, 829, 1166
KasI GGCGCC 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 5 cut(s) 553, 736, 1057, 1199, 1226
LguI GCTCTTC 2 cut(s) 126, 531
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 5 cut(s) 181, 594, 949, 1004, 1180
LweI GCATC 4 cut(s) 181, 505, 988, 1127
MaeI CTAG 3 cut(s) 707, 1137, 1203
MaeII ACGT 3 cut(s) 87, 317, 933
MaeIII GTNAC 1 cut(s) 794
MalI GATC 5 cut(s) 555, 738, 1059, 1201, 1228
MboI GATC 5 cut(s) 553, 736, 1057, 1199, 1226
MflI RGATCY 1 cut(s) 1226
MluCI AATT 1 cut(s) 927
Mly113I GGCGCC 1 cut(s) 278
MlyI GAGTC 3 cut(s) 239, 874, 1105
MmeI TCCRAC 2 cut(s) 48, 72
Mph1103I ATGCAT 1 cut(s) 1120
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 2 cut(s) 584, 930
MslI CAYNNNNRTG 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 2 cut(s) 264, 648
MspR9I CCNGG 5 cut(s) 265, 405, 432, 649, 903
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 3 cut(s) 405, 432, 903
MvnI CGCG 1 cut(s) 831
MwoI GCNNNNNNNGC 2 cut(s) 524, 998
NarI GGCGCC 1 cut(s) 278
NciI CCSGG 2 cut(s) 265, 649
NcoI CCATGG 1 cut(s) 957
NdeII GATC 5 cut(s) 553, 736, 1057, 1199, 1226
NlaIII CATG 6 cut(s) 419, 716, 782, 961, 1118, 1159
NlaIV GGNNCC 2 cut(s) 279, 916
NmuCI GTSAC 1 cut(s) 794
NsbI TGCGCA 1 cut(s) 1167
NsiI ATGCAT 1 cut(s) 1120
NspI RCATGY 1 cut(s) 716
PaeI GCATGC 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 2 cut(s) 126, 531
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 500, 694, 1091
PfoI TCCNGGA 1 cut(s) 430
PkrI GCNGC 7 cut(s) 196, 520, 609, 919, 964, 994, 1170
PleI GAGTC 3 cut(s) 238, 873, 1105
PluTI GGCGCC 1 cut(s) 281
PpsI GAGTC 3 cut(s) 238, 873, 1105
Psp6I CCWGG 3 cut(s) 403, 430, 901
PspGI CCWGG 3 cut(s) 403, 430, 901
PspN4I GGNNCC 2 cut(s) 279, 916
PspXI VCTCGAGB 1 cut(s) 7
PstI CTGCAG 1 cut(s) 994
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 1226
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 2 cut(s) 495, 936
RsaNI GTAC 2 cut(s) 494, 935
RseI CAYNNNNRTG 1 cut(s) 783
SapI GCTCTTC 2 cut(s) 126, 531
SaqAI TTAA 2 cut(s) 584, 930
SatI GCNGC 7 cut(s) 195, 519, 608, 918, 963, 993, 1169
Sau3AI GATC 5 cut(s) 553, 736, 1057, 1199, 1226
SchI GAGTC 3 cut(s) 239, 874, 1105
ScrFI CCNGG 5 cut(s) 265, 405, 432, 649, 903
SfaNI GCATC 4 cut(s) 181, 505, 988, 1127
SfcI CTRYAG 1 cut(s) 990
SfoI GGCGCC 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SphI GCATGC 1 cut(s) 716
Sse9I AATT 1 cut(s) 927
SsiI CCGC 5 cut(s) 254, 519, 575, 716, 918
SspDI GGCGCC 1 cut(s) 277
SspMI CTAG 3 cut(s) 707, 1137, 1203
StyD4I CCNGG 5 cut(s) 263, 403, 430, 647, 901
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 8 cut(s) 167, 220, 439, 465, 481, 493, 803, 1105
TaiI ACGT 3 cut(s) 90, 320, 936
TaqI TCGA 3 cut(s) 8, 120, 1060
TasI AATT 1 cut(s) 927
TatI WGTACW 1 cut(s) 493
TauI GCSGC 2 cut(s) 521, 920
TfiI GAWTC 3 cut(s) 500, 694, 1091
Tru1I TTAA 2 cut(s) 584, 930
Tru9I TTAA 2 cut(s) 584, 930
TscAI CASTG 1 cut(s) 486
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 5 cut(s) 194, 607, 962, 992, 1168
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 4 cut(s) 87, 144, 360, 822
TspGWI ACGGA 2 cut(s) 160, 1192
TspRI CASTG 1 cut(s) 486
XagI CCTNNNNNAGG 1 cut(s) 942
XceI RCATGY 1 cut(s) 716
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 1018, 1100
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 3 cut(s) 707, 1137, 1203
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.