RchiOBHm_Chr5g0011231

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008773
Physical Location & Seq
Forward (+)
7444625 .. 7449366
4742 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29192

Sequence Viewer

Length: 1227 bp
ATGGCCTCGAGAAAGCGGCAATTCGAGGGCATCGCGATGCTGTCGATGTACAACGACGAGGAAGAAGAAGAAGAAGAAGAAGAGGAAGAGGACGAAGACATGGACGACGCTGAAGAACGCGGCGAACAAGAAGAGCGCGGCGGAGAGAGAGAAGAAGGAGAACGGCGGGAACACGGCGGCGATGCGTCCATGTTGGATGTCGATGCGACTGCGGCCGTTGATTCTGGTAACGATGACAATGCAACGAGCGGTGTGGATGATGAGAACTCGGCGCGGAGTGAAGGGCAGTTTCGTCGTTCTTCGTCGCAACAGCAGCTCTCTCAGGCGACGGGATCGCCGCAGCAAGTGGCGCCGGGGAAGTTGAGTAGGAGAGGGACGCTTGTGGACTATGGCCATGATGAAGTTGCCATGTCTCCAGAGCCTGAGGAGGGTGAAATTGATGGAAGTGGTCGCATTTTGTCTACCAATGGTGATTCACATGATAAGACACCTCAGTTAAGCAATCAAGTAACTCCTCAAATATCTGAGCCGTCACAATCTGAAACACTGAATGAGACTATACGTGAATCAGAGGGTAGAAATGCTACAGAGGCTGTTGCAGAAGATCAAAAAAATGTTGATCCATTAGATAAGTTTCTTCCACCGCCATTAAAGCTCAAGTGCTCGGAGGAGCTGCAAAGAAAGATTCAAAAGTTTCTTGAATTAAAGAGAGCTGGGAAAAGCTTCAATGAAGAAGTGCGGAAAAAGAAGGACTATAGGAATCCAGACTTTCTATTGCATGCAGTGCGGTATCAAGATATTGATCAGATAGGTTCATGCTTCAGTAAAGATGTATTTGACCCTCATGGATTTGACAAAAGTGACTACTATGATGAAATAGAGGCTGATTCGAGGCGTGAAATGGAAAGGGTTAATCAAGAAAAAAAGAACCGTCAGAATCTTGAATTTGTTTCTGGTGGAAGACAATCTACAATAGTTGGTGCTGCACCTAAAATTAATGTACCTCTTCCAGGTATAACTTCTGGGTTGAGTTCCTTACCGCCTGCTGCTGATATCATCCCTCGGGACGGTAGACAGAATAAGAAGTCGAAGTGGGATAAGATTGATGGGACTCATGCAACACTCTTATCTTCTGCTAATGCTGGTGGGTACACTTTGGGGCAGCAGAAACGGAGAGATGTGGAGGAGAAGAGATCTAGTGAAAGGAAGTTAGACAGAAGATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

408

Amino Acids

45.68

Weight (kDa)

4.75

Isoelectric Point (pI)

66.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 213 - 305 3.4e-29 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 349
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 2 cut(s) 461, 1072
AccII CGCG 4 cut(s) 35, 120, 138, 274
AclWI GGATC 2 cut(s) 340, 614
AcoI YGGCCR 2 cut(s) 213, 391
AcsI RAATTY 1 cut(s) 944
AcuI CTGAAG 2 cut(s) 132, 805
AcyI GRCGYC 1 cut(s) 350
AfaI GTAC 3 cut(s) 50, 1002, 1151
AfiI CCNNNNNNNGG 3 cut(s) 428, 1010, 1067
AgsI TTSAA 4 cut(s) 689, 701, 727, 944
AjnI CCWGG 1 cut(s) 1009
AluBI AGCT 5 cut(s) 316, 655, 673, 713, 723
AluI AGCT 5 cut(s) 316, 655, 673, 713, 723
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 2 cut(s) 417, 548
AlwI GGATC 2 cut(s) 340, 614
AlwNI CAGNNNCTG 2 cut(s) 422, 593
Ama87I CYCGRG 2 cut(s) 7, 1062
AoxI GGCC 3 cut(s) 3, 213, 391
ApeKI GCWGC 6 cut(s) 313, 340, 673, 983, 1046, 1162
ApoI RAATTY 1 cut(s) 944
AseI ATTAAT 1 cut(s) 996
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 3 cut(s) 138, 274, 352
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 443, 482
AvaI CYCGRG 2 cut(s) 7, 1062
AxyI CCTNAGG 1 cut(s) 423
BalI TGGCCA 1 cut(s) 393
BanI GGYRCC 1 cut(s) 349
BarI GAAGNNNNNNTAC 2 cut(s) 952, 984
BbsI GAAGAC 2 cut(s) 102, 967
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 6 cut(s) 325, 352, 660, 970, 1033, 1174
BccI CCATC 2 cut(s) 434, 1100
BceAI ACGGC 4 cut(s) 179, 190, 200, 514
BcgI CGANNNNNNTGC 6 cut(s) 191, 221, 225, 255, 275, 309
BciT130I CCWGG 1 cut(s) 1011
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 2 cut(s) 417, 548
BfaI CTAG 1 cut(s) 1197
BfmI CTRYAG 2 cut(s) 585, 754
BfoI RGCGCY 1 cut(s) 353
BglII AGATCT 1 cut(s) 1193
Bme1390I CCNGG 2 cut(s) 354, 1011
BmeT110I CYCGRG 2 cut(s) 7, 1062
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 354, 1011
BmsI GCATC 4 cut(s) 27, 39, 172, 193
BpiI GAAGAC 2 cut(s) 102, 967
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 801
BsaHI GRCGYC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 353, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 1010, 1067
Bse21I CCTNAGG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 1 cut(s) 1011
BseDI CCNNGG 2 cut(s) 353, 1061
BseGI GGATG 3 cut(s) 202, 262, 1056
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 3 cut(s) 428, 1010, 1067
BseMII CTCAG 4 cut(s) 335, 414, 506, 516
BseRI GAGGAG 4 cut(s) 440, 504, 683, 1199
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 6 cut(s) 325, 352, 660, 970, 1033, 1174
BseYI CCCAGC 1 cut(s) 713
BsgI GTGCAG 1 cut(s) 969
Bsh1236I CGCG 4 cut(s) 35, 120, 138, 274
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 3 cut(s) 5, 215, 393
BshNI GGYRCC 1 cut(s) 349
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 665
BsiHKCI CYCGRG 2 cut(s) 7, 1062
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 3 cut(s) 388, 1079, 1123
BslI CCNNNNNNNGG 3 cut(s) 428, 1010, 1067
BsmAI GTCTC 2 cut(s) 417, 548
BsmFI GGGAC 3 cut(s) 388, 1079, 1123
BsnI GGCC 3 cut(s) 5, 215, 393
BsoBI CYCGRG 2 cut(s) 7, 1062
Bsp1286I GDGCHC 1 cut(s) 665
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
Bsp68I TCGCGA 1 cut(s) 35
BspANI GGCC 3 cut(s) 5, 215, 393
BspCNI CTCAG 4 cut(s) 334, 415, 505, 517
BspFNI CGCG 4 cut(s) 35, 120, 138, 274
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 2 cut(s) 340, 614
BspQI GCTCTTC 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 353, 1061
BssMI GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
BssNI GRCGYC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 1011
Bst4CI ACNGT 2 cut(s) 932, 1070
Bst6I CTCTTC 5 cut(s) 75, 81, 126, 1011, 1184
BstACI GRCGYC 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 784
BstAUI TGTACA 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 780, 1044
BstDEI CTNAG 4 cut(s) 321, 423, 492, 525
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 3 cut(s) 202, 262, 1056
BstFNI CGCG 4 cut(s) 35, 120, 138, 274
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 3 cut(s) 138, 274, 352
BstKTI GATC 6 cut(s) 335, 607, 622, 805, 1196, 1223
BstMAI GTCTC 2 cut(s) 417, 548
BstMBI GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 6 cut(s) 212, 313, 349, 590, 652, 784
BstNI CCWGG 1 cut(s) 1011
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 352, 1009
BstSFI CTRYAG 2 cut(s) 585, 754
BstUI CGCG 4 cut(s) 35, 120, 138, 274
BstV1I GCAGC 6 cut(s) 325, 352, 660, 970, 1033, 1174
BstV2I GAAGAC 2 cut(s) 102, 967
BstX2I RGATCY 1 cut(s) 1193
BstYI RGATCY 1 cut(s) 1193
BstZI CGGCCG 1 cut(s) 213
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 3 cut(s) 5, 215, 393
BtgZI GCGATG 3 cut(s) 16, 50, 195
BtsCI GGATG 3 cut(s) 202, 262, 1056
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 545, 789
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 780, 1044
CaiI CAGNNNCTG 2 cut(s) 422, 593
CfoI GCGC 3 cut(s) 138, 274, 352
CseI GACGC 3 cut(s) 116, 174, 385
Csp6I GTAC 3 cut(s) 49, 1001, 1150
CviAII CATG 9 cut(s) 100, 190, 395, 409, 479, 779, 816, 845, 1115
CviQI GTAC 3 cut(s) 49, 1001, 1150
DdeI CTNAG 4 cut(s) 321, 423, 492, 525
DinI GGCGCC 1 cut(s) 351
DpnI GATC 6 cut(s) 334, 606, 621, 804, 1195, 1222
DpnII GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
EaeI YGGCCR 2 cut(s) 213, 391
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 5 cut(s) 75, 81, 126, 1011, 1184
EarI CTCTTC 5 cut(s) 75, 81, 126, 1011, 1184
EciI GGCGGA 1 cut(s) 156
EclXI CGGCCG 1 cut(s) 213
Eco32I GATATC 1 cut(s) 1054
Eco52I CGGCCG 1 cut(s) 213
Eco57I CTGAAG 2 cut(s) 132, 805
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 2 cut(s) 7, 1062
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoRII CCWGG 1 cut(s) 1009
EcoRV GATATC 1 cut(s) 1054
EgeI GGCGCC 1 cut(s) 351
EheI GGCGCC 1 cut(s) 351
FaeI CATG 9 cut(s) 103, 193, 398, 412, 482, 782, 819, 848, 1118
FaqI GGGAC 3 cut(s) 388, 1079, 1123
FatI CATG 9 cut(s) 99, 189, 394, 408, 478, 778, 815, 844, 1114
FauI CCCGC 1 cut(s) 159
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 2 cut(s) 461, 1072
FokI GGATG 3 cut(s) 209, 269, 1043
FspBI CTAG 1 cut(s) 1197
GlaI GCGC 3 cut(s) 137, 273, 351
GsaI CCCAGC 1 cut(s) 717
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 3 cut(s) 5, 215, 393
HapII CCGG 1 cut(s) 353
HgaI GACGC 3 cut(s) 116, 174, 385
HhaI GCGC 3 cut(s) 138, 274, 352
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 9 cut(s) 103, 193, 398, 412, 482, 782, 819, 848, 1118
Hin6I GCGC 3 cut(s) 136, 272, 350
HinP1I GCGC 3 cut(s) 136, 272, 350
HindIII AAGCTT 1 cut(s) 721
HinfI GANTC 8 cut(s) 221, 473, 566, 685, 760, 887, 937, 1111
HpaII CCGG 1 cut(s) 353
HphI GGTGA 2 cut(s) 443, 482
Hpy166II GTNNAC 4 cut(s) 385, 462, 1073, 1152
Hpy188I TCNGA 6 cut(s) 526, 541, 571, 667, 807, 936
Hpy8I GTNNAC 4 cut(s) 385, 462, 1073, 1152
Hpy99I CGWCG 5 cut(s) 59, 110, 297, 307, 331
HpyAV CCTTC 3 cut(s) 149, 275, 742
HpyCH4III ACNGT 2 cut(s) 932, 1070
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 7 cut(s) 242, 599, 676, 778, 782, 986, 1118
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 313, 349, 590, 652, 784
HpyF3I CTNAG 4 cut(s) 321, 423, 492, 525
HpySE526I ACGT 1 cut(s) 562
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 9 cut(s) 103, 193, 398, 412, 482, 782, 819, 848, 1118
HspAI GCGC 3 cut(s) 136, 272, 350
KasI GGCGCC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 6 cut(s) 325, 352, 660, 970, 1033, 1174
LweI GCATC 4 cut(s) 27, 39, 172, 193
MaeI CTAG 1 cut(s) 1197
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 4 cut(s) 227, 508, 531, 860
MalI GATC 6 cut(s) 334, 606, 621, 804, 1195, 1222
MbiI CCGCTC 1 cut(s) 249
MboI GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
MflI RGATCY 1 cut(s) 1193
MhlI GDGCHC 1 cut(s) 665
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 5 cut(s) 20, 435, 701, 944, 993
MluNI TGGCCA 1 cut(s) 393
Mly113I GGCGCC 1 cut(s) 350
MlyI GAGTC 1 cut(s) 1105
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 393
MroXI GAANNNNTTC 1 cut(s) 722
MscI TGGCCA 1 cut(s) 393
MseI TTAA 5 cut(s) 497, 650, 704, 912, 996
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 393
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 354, 1011
MvaI CCWGG 1 cut(s) 1011
MvnI CGCG 4 cut(s) 35, 120, 138, 274
MwoI GCNNNNNNNGC 6 cut(s) 212, 313, 349, 590, 652, 784
NarI GGCGCC 1 cut(s) 350
NciI CCSGG 1 cut(s) 354
NdeII GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
NlaIII CATG 9 cut(s) 103, 193, 398, 412, 482, 782, 819, 848, 1118
NlaIV GGNNCC 1 cut(s) 351
NmeAIII GCCGAG 1 cut(s) 248
NmuCI GTSAC 2 cut(s) 531, 860
NruI TCGCGA 1 cut(s) 35
NspI RCATGY 1 cut(s) 782
PaeI GCATGC 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 7 cut(s) 221, 473, 566, 685, 760, 887, 937
PleI GAGTC 1 cut(s) 1105
PluTI GGCGCC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 1105
Ppu21I YACGTR 1 cut(s) 563
PshBI ATTAAT 1 cut(s) 996
Psp6I CCWGG 1 cut(s) 1009
PspFI CCCAGC 1 cut(s) 713
PspGI CCWGG 1 cut(s) 1009
PspN4I GGNNCC 1 cut(s) 351
PstNI CAGNNNCTG 2 cut(s) 422, 593
PsuI RGATCY 1 cut(s) 1193
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 3 cut(s) 50, 1002, 1151
RsaNI GTAC 3 cut(s) 49, 1001, 1150
RseI CAYNNNNRTG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 5 cut(s) 497, 650, 704, 912, 996
Sau3AI GATC 6 cut(s) 332, 604, 619, 802, 1193, 1220
SchI GAGTC 1 cut(s) 1105
ScrFI CCNGG 2 cut(s) 354, 1011
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 4 cut(s) 27, 39, 172, 193
SfcI CTRYAG 2 cut(s) 585, 754
SfoI GGCGCC 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 7, 656
SmoI CTYRAG 2 cut(s) 7, 656
SphI GCATGC 1 cut(s) 782
Sse9I AATT 5 cut(s) 20, 435, 701, 944, 993
SspDI GGCGCC 1 cut(s) 349
SspMI CTAG 1 cut(s) 1197
StyD4I CCNGG 2 cut(s) 352, 1009
TaaI ACNGT 2 cut(s) 932, 1070
TaiI ACGT 1 cut(s) 565
TaqI TCGA 6 cut(s) 8, 24, 44, 201, 890, 1088
TasI AATT 5 cut(s) 20, 435, 701, 944, 993
TatI WGTACW 1 cut(s) 48
TauI GCSGC 6 cut(s) 19, 123, 141, 180, 215, 340
TfiI GAWTC 7 cut(s) 221, 473, 566, 685, 760, 887, 937
Tru1I TTAA 5 cut(s) 497, 650, 704, 912, 996
Tru9I TTAA 5 cut(s) 497, 650, 704, 912, 996
TscAI CASTG 2 cut(s) 552, 789
TseFI GTSAC 2 cut(s) 531, 860
TseI GCWGC 6 cut(s) 313, 340, 673, 983, 1046, 1162
Tsp45I GTSAC 2 cut(s) 531, 860
TspDTI ATGAA 4 cut(s) 414, 744, 804, 888
TspGWI ACGGA 1 cut(s) 1186
TspRI CASTG 2 cut(s) 552, 789
VspI ATTAAT 1 cut(s) 996
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 1 cut(s) 944
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 461, 1072
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 1 cut(s) 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.