MD05G1298600.v1.1

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008773
Physical Location & Seq
Reverse (-)
43199757 .. 43203062
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1298600.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGCATCGAGGAAGAAGCAATCCGACGCCATAGCTCTCCTGCTCTCCAATTACAACGACGAAGAAGAAGATGAAGACATGGAGGACGTTGAGCAGCACAACAACAAAGAAGAAGAAGGAGATGAAGAGCGACGAGAACGGGGAGAATTCGACGATTACGGTGAGCTGAGAGGCTCGGGAGATGCTGCAATGGCGGATGTGGATGGAACGGTGGGTGGTGAGTCTAGGAACGACGATAGCGCACCGCCGAATGCCGGGGATAATGAGGTGGCTTCGGATGGTTTGAATAGAAGAAGAAGAGGGGCACTTACAATTGTGGACTATGGGCATGATGAAGTTGCTATGTCTCCTGAGCCTGAGGAGGGTGAAATAGAAGGGATTGGTCGTGTTTCTGGTGATTTCCTTGATAAGACACCTCCAGGAACAGTTCATACTCCTCAATTATCTGAACCGTCAAACTCTGACACAATGAACGATATTGTACTTGAATCTGAGGGTAGAGATGCTGCAGAGTCTGTTGCAGAAGAGCAGAAAGATATTGTTCTGTTGGATAAGTTTCTCCCGCCACCATCAAAAGCAAAGTGTTCAGAGGAGCTGCAAAGGAGGATAAATAAGTTTCTTGAATACAAGAGAGCTGGGAAAAGCTTCAATGCAGAAGTGCGTAATAAGAAGGACTATAGGAATCCAAACTTTCTGGTGCATGCAGTGAGGTATCAAGATATTGATCAGATAGGGTCTTGCTTCAGTAAAGATGTATTTGACCCTCATGGATATGACAAAAGTGACTATTGTGATGAAATAGAGGCTGATATGAGGTGTGAAATGGAAAGGAAAGAGCAAGATAGGAAGAAGAGTCAAAAGGTTGAATATGTTTCAGGAGGAACACAGCCTGGAATAGTTGGTGTTGCACCTAAAATTAACATACCTTATCCAGGTGTTTCAACCATGGCTGCTACTGGGTTGAGCTCCTTACCGCCTGCAGCTGATGTCATCCCTCGGGAGGGTAGACAGAACAAGAAGTCAAAATGGGATAAGGTCGATGGTGATCGAAAAAATCCTCTTCCCTCAGGACCGCAGGATTCTATGTCTACTCTGGGAACTCATGCAACACTCTTATCTTCTGCCAGTGCTGGTGCTGGATACATGGCTTTTGCGCAGCAGAGACGAAAAGAGGTGGAGGAGAAACGATCAAGTGAAAGGAAGTTAGATCGAAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

407

Amino Acids

45.01

Weight (kDa)

4.8

Isoelectric Point (pI)

60.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 187 - 280 2.1e-28 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1155
AccI GTMKAC 2 cut(s) 1006, 1088
AciI CCGC 5 cut(s) 194, 245, 563, 974, 1073
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 727
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 5 cut(s) 254, 362, 932, 1000, 1001
AgsI TTSAA 6 cut(s) 286, 488, 623, 649, 866, 942
AjnI CCWGG 3 cut(s) 418, 889, 931
AluBI AGCT 7 cut(s) 35, 166, 595, 635, 645, 966, 983
AluI AGCT 7 cut(s) 35, 166, 595, 635, 645, 966, 983
Alw21I GWGCWC 1 cut(s) 968
Alw26I GTCTC 2 cut(s) 351, 1156
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 2 cut(s) 175, 996
ApeKI GCWGC 7 cut(s) 94, 185, 506, 595, 950, 980, 1156
ApoI RAATTY 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 2 cut(s) 242, 1156
AspS9I GGNCC 1 cut(s) 1070
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 5 cut(s) 173, 230, 377, 407, 1055
AvaI CYCGRG 2 cut(s) 175, 996
AvaII GGWCC 1 cut(s) 1070
AxyI CCTNAGG 2 cut(s) 357, 1066
BaeGI GKGCMC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 968
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 968
BbvI GCAGC 7 cut(s) 106, 172, 493, 582, 937, 992, 1168
BccI CCATC 4 cut(s) 197, 272, 577, 1034
BciT130I CCWGG 3 cut(s) 420, 891, 933
BciVI GTATCC 1 cut(s) 1133
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 351, 1156
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 3 cut(s) 507, 676, 978
BfuI GTATCC 1 cut(s) 1133
BglII AGATCT 1 cut(s) 1214
BisI GCNGC 7 cut(s) 95, 186, 507, 596, 951, 981, 1157
BlsI GCNGC 7 cut(s) 96, 187, 508, 597, 952, 982, 1158
Bme1390I CCNGG 4 cut(s) 256, 420, 891, 933
Bme18I GGWCC 1 cut(s) 1070
BmeT110I CYCGRG 2 cut(s) 175, 996
BmgT120I GGNCC 1 cut(s) 1070
BmrFI CCNGG 4 cut(s) 256, 420, 891, 933
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 3 cut(s) 14, 172, 493
BmuI ACTGGG 1 cut(s) 966
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 351
BpuMI CCSGG 1 cut(s) 256
BsaBI GATNNNNATC 2 cut(s) 723, 1044
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 255, 945, 995
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 362, 932, 1000, 1001
Bse1I ACTGG 2 cut(s) 961, 1125
Bse21I CCTNAGG 2 cut(s) 357, 1066
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 2 cut(s) 723, 1044
BseBI CCWGG 3 cut(s) 420, 891, 933
BseDI CCNNGG 3 cut(s) 255, 945, 995
BseGI GGATG 4 cut(s) 202, 208, 283, 990
BseJI GATNNNNATC 2 cut(s) 723, 1044
BseLI CCNNNNNNNGG 5 cut(s) 254, 362, 932, 1000, 1001
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 5 cut(s) 158, 342, 348, 483, 1080
BseNI ACTGG 2 cut(s) 961, 1125
BseRI GAGGAG 4 cut(s) 374, 426, 605, 1193
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 7 cut(s) 106, 172, 493, 582, 937, 992, 1168
BseYI CCCAGC 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 968
BsiHKCI CYCGRG 2 cut(s) 175, 996
BsiSI CCGG 1 cut(s) 255
BslI CCNNNNNNNGG 5 cut(s) 254, 362, 932, 1000, 1001
BsmAI GTCTC 2 cut(s) 351, 1156
BsmBI CGTCTC 1 cut(s) 1156
BsmI GAATGC 1 cut(s) 256
BsoBI CYCGRG 2 cut(s) 175, 996
Bsp1286I GDGCHC 2 cut(s) 307, 968
Bsp143I GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 5 cut(s) 194, 245, 563, 974, 1073
BspCNI CTCAG 5 cut(s) 159, 343, 349, 484, 1079
BspMAI CTGCAG 2 cut(s) 511, 982
BspQI GCTCTTC 2 cut(s) 120, 519
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 2 cut(s) 961, 1125
BssECI CCNNGG 3 cut(s) 255, 945, 995
BssMI GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 3 cut(s) 420, 891, 933
Bst4CI ACNGT 4 cut(s) 161, 211, 427, 453
Bst6I CTCTTC 5 cut(s) 120, 292, 519, 845, 1065
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 702, 978
BstDEI CTNAG 5 cut(s) 167, 351, 357, 492, 1066
BstDSI CCRYGG 1 cut(s) 945
BstENI CCTNNNNNAGG 1 cut(s) 930
BstF5I GGATG 4 cut(s) 202, 208, 283, 990
BstHHI GCGC 2 cut(s) 242, 1156
BstKTI GATC 5 cut(s) 727, 1048, 1190, 1210, 1217
BstMAI GTCTC 2 cut(s) 351, 1156
BstMBI GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 3 cut(s) 420, 891, 933
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 4 cut(s) 254, 418, 889, 931
BstSFI CTRYAG 3 cut(s) 507, 676, 978
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 7 cut(s) 106, 172, 493, 582, 937, 992, 1168
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 1214
BstYI RGATCY 1 cut(s) 1214
Bsu36I CCTNAGG 2 cut(s) 357, 1066
BsuI GTATCC 1 cut(s) 1133
BtgI CCRYGG 1 cut(s) 945
BtsCI GGATG 4 cut(s) 202, 208, 283, 990
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 711, 1132
Cac8I GCNNGC 2 cut(s) 702, 978
CaiI CAGNNNCTG 1 cut(s) 515
CfoI GCGC 2 cut(s) 242, 1156
Cfr13I GGNCC 1 cut(s) 1070
CseI GACGC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 482
CviAII CATG 7 cut(s) 79, 329, 701, 767, 946, 1103, 1144
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 5 cut(s) 167, 351, 357, 492, 1066
DpnI GATC 5 cut(s) 726, 1047, 1189, 1209, 1216
DpnII GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
Eam1104I CTCTTC 5 cut(s) 120, 292, 519, 845, 1065
EarI CTCTTC 5 cut(s) 120, 292, 519, 845, 1065
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 966
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 1 cut(s) 968
Eco47I GGWCC 1 cut(s) 1070
Eco53kI GAGCTC 1 cut(s) 966
Eco57I CTGAAG 1 cut(s) 727
Eco81I CCTNAGG 2 cut(s) 357, 1066
Eco88I CYCGRG 2 cut(s) 175, 996
EcoICRI GAGCTC 1 cut(s) 966
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 418, 889, 931
EcoT14I CCWWGG 1 cut(s) 945
EcoT38I GRGCYC 1 cut(s) 968
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 1156
FaeI CATG 7 cut(s) 82, 332, 704, 770, 949, 1106, 1147
FatI CATG 7 cut(s) 78, 328, 700, 766, 945, 1102, 1143
FauI CCCGC 1 cut(s) 570
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 2 cut(s) 1006, 1088
Fnu4HI GCNGC 7 cut(s) 95, 186, 507, 596, 951, 981, 1157
FokI GGATG 4 cut(s) 209, 215, 290, 977
FriOI GRGCYC 1 cut(s) 968
Fsp4HI GCNGC 7 cut(s) 95, 186, 507, 596, 951, 981, 1157
FspBI CTAG 1 cut(s) 225
FspI TGCGCA 1 cut(s) 1155
GlaI GCGC 2 cut(s) 241, 1155
GluI GCNGC 7 cut(s) 95, 186, 507, 596, 951, 981, 1157
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 402
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 35
HhaI GCGC 2 cut(s) 242, 1156
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 7 cut(s) 82, 332, 704, 770, 949, 1106, 1147
Hin6I GCGC 2 cut(s) 240, 1154
HinP1I GCGC 2 cut(s) 240, 1154
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 6 cut(s) 221, 488, 512, 682, 853, 1079
HpaII CCGG 1 cut(s) 255
HphI GGTGA 5 cut(s) 173, 230, 377, 407, 1055
Hpy166II GTNNAC 3 cut(s) 319, 1007, 1089
Hpy188I TCNGA 7 cut(s) 25, 277, 448, 463, 493, 589, 729
Hpy188III TCNNGA 8 cut(s) 177, 350, 620, 716, 876, 998, 1068, 1218
Hpy8I GTNNAC 3 cut(s) 319, 1007, 1089
Hpy99I CGWCG 5 cut(s) 29, 62, 135, 155, 236
HpyAV CCTTC 3 cut(s) 110, 368, 664
HpyCH4III ACNGT 4 cut(s) 161, 211, 427, 453
HpyCH4IV ACGT 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 5 cut(s) 167, 351, 357, 492, 1066
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 7 cut(s) 82, 332, 704, 770, 949, 1106, 1147
HspAI GCGC 2 cut(s) 240, 1154
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
LguI GCTCTTC 2 cut(s) 120, 519
LmnI GCTCC 2 cut(s) 592, 971
Lsp1109I GCAGC 7 cut(s) 106, 172, 493, 582, 937, 992, 1168
LweI GCATC 3 cut(s) 14, 172, 493
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 782
MalI GATC 5 cut(s) 726, 1047, 1189, 1209, 1216
MboI GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 1214
MhlI GDGCHC 2 cut(s) 307, 968
MluCI AATT 5 cut(s) 49, 146, 312, 440, 915
MlyI GAGTC 3 cut(s) 230, 521, 862
MmeI TCCRAC 2 cut(s) 48, 528
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 1 cut(s) 918
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 4 cut(s) 256, 420, 891, 933
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 420, 891, 933
MwoI GCNNNNNNNGC 1 cut(s) 191
NciI CCSGG 1 cut(s) 256
NcoI CCATGG 1 cut(s) 945
NdeII GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
NlaIII CATG 7 cut(s) 82, 332, 704, 770, 949, 1106, 1147
NmuCI GTSAC 1 cut(s) 782
NsbI TGCGCA 1 cut(s) 1155
NspI RCATGY 1 cut(s) 704
PaeI GCATGC 1 cut(s) 704
PciSI GCTCTTC 2 cut(s) 120, 519
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 488, 682, 1079
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 7 cut(s) 96, 187, 508, 597, 952, 982, 1158
PleI GAGTC 3 cut(s) 229, 520, 861
PpsI GAGTC 3 cut(s) 229, 520, 861
Psp124BI GAGCTC 1 cut(s) 968
Psp6I CCWGG 3 cut(s) 418, 889, 931
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 3 cut(s) 418, 889, 931
PspPI GGNCC 1 cut(s) 1070
PsrI GAACNNNNNNTAC 2 cut(s) 415, 447
PstI CTGCAG 2 cut(s) 511, 982
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 1214
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 968
SapI GCTCTTC 2 cut(s) 120, 519
SaqAI TTAA 1 cut(s) 918
SatI GCNGC 7 cut(s) 95, 186, 507, 596, 951, 981, 1157
Sau3AI GATC 5 cut(s) 724, 1045, 1187, 1207, 1214
Sau96I GGNCC 1 cut(s) 1070
SchI GAGTC 3 cut(s) 230, 521, 862
ScrFI CCNGG 4 cut(s) 256, 420, 891, 933
SduI GDGCHC 2 cut(s) 307, 968
SfaNI GCATC 3 cut(s) 14, 172, 493
SfcI CTRYAG 3 cut(s) 507, 676, 978
SinI GGWCC 1 cut(s) 1070
SmiMI CAYNNNNRTG 1 cut(s) 771
SphI GCATGC 1 cut(s) 704
Sse9I AATT 5 cut(s) 49, 146, 312, 440, 915
SsiI CCGC 5 cut(s) 194, 245, 563, 974, 1073
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 968
StyD4I CCNGG 4 cut(s) 254, 418, 889, 931
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 4 cut(s) 161, 211, 427, 453
TaiI ACGT 1 cut(s) 90
TaqI TCGA 5 cut(s) 8, 150, 1038, 1048, 1210
TasI AATT 5 cut(s) 49, 146, 312, 440, 915
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 3 cut(s) 488, 682, 1079
Tru1I TTAA 1 cut(s) 918
Tru9I TTAA 1 cut(s) 918
TscAI CASTG 2 cut(s) 711, 1132
TseFI GTSAC 1 cut(s) 782
TseI GCWGC 7 cut(s) 94, 185, 506, 595, 950, 980, 1156
Tsp45I GTSAC 1 cut(s) 782
TspDTI ATGAA 6 cut(s) 87, 138, 348, 419, 485, 810
TspRI CASTG 2 cut(s) 711, 1132
VpaK11BI GGWCC 1 cut(s) 1070
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 1 cut(s) 146
XceI RCATGY 1 cut(s) 704
XmiI GTMKAC 2 cut(s) 1006, 1088
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.