Rroxscaffold_1G00065170

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008773
Physical Location & Seq
Reverse (-)
86929287 .. 86934183
4897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065170.1

Sequence Viewer

Length: 1128 bp
ATGGCCTCGAGAAAGCGGCAATTCGAGGGCATCGCGATGCTGTCGATGTACAACGACGAGGAAGAAGAAGAAGAAGAAGAAGAGGACGAAGACATGGACGTCGCTGAAGAACGCGGCGAACAAGAAGAGCGCGGCGAGAGAGAGAAGACGGAGAACGGCGGGAACACGGCGGCGATGCCTCCACGTTGGATGTCGATGCAACGGCGGCCGTTGATTCTGCAGCTCTCTCAGGCGACGGGATCGCCGCAGCAAGTGGCGCCGGGGAAGTTGGGTAGGAGAGGGACGCTTGTGGACTATGGCCATGATGAAGTTGCCATGTCTCCAGAGCCTGAGGAGGGTGAAATTGATGGAAGTGGTCGCATTTTGTCTACCAATGGTGATTCACATGATAAGACACCTCAGTTAAGCAATCAAGTAACTCCTCAAATATCTGAGCCATCACAATCTGAAACACTGAATGAGACTCTACTTGAATCAGAGGGTAGAAATGCTACAGAGGCTGTTGCAGAAGATCAAAAAAATGTTGATCCATTAGATAAGTTTCTTCCATCACCATTAAAGCTCAAGTGCTCAGAGGAGCTGCAAAGAAAGATTCAAAAGTTTCTTGAATTAAAGAGAGCTGGGAAAAGCTTCAATGAAGAAGTGCGGAAAAAGAAGGACTATAGGAATCCAGACTTTCTATTGCATGCAGTGCGGTATCAAGATATTGATCAGATAGGTTCATGCTTCAGTAAAGATGTATTCGACCCTCATGGATTTGACAAAAGTGACTACTATGATGAAATAGGTATAACTTCTGGGTTGAGTTCCTTACCGCCTGCTGCTGATATCATCCCTCGGGACGGTAGACAGAATAAGAAGTCGAAGTGGGATAAGATTGATGGGACTCATGCAACACTCTTATCTTCTGCTAATACTGGTGGATACAGTTTGGGGCAGCAGAAACGGAGAGATGTGGAGGAGAAGAGATCTAGTGAAAGGAAGTTAGACAGAAGATCGAAGGAAAAGTTACGAGCTGAATTACACTACCGCAATAGCCATAAAAGTTTGCTTCCAAGTTCTAACTCAAATTCAGATGGAGCTGGAAATAGCCATAAAAGTTTGCTTCTGAGTTCTAACAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

375

Amino Acids

42.26

Weight (kDa)

5.26

Isoelectric Point (pI)

66.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 183 - 262 2.6e-26 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AatII GACGTC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 368, 847
AccII CGCG 3 cut(s) 35, 114, 132
AclWI GGATC 2 cut(s) 247, 521
AcoI YGGCCR 2 cut(s) 206, 298
AcsI RAATTY 1 cut(s) 1069
AcuI CTGAAG 2 cut(s) 126, 712
AcyI GRCGYC 2 cut(s) 99, 257
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 335, 842
AgsI TTSAA 4 cut(s) 473, 596, 608, 634
AluBI AGCT 7 cut(s) 223, 562, 580, 620, 630, 1016, 1082
AluI AGCT 7 cut(s) 223, 562, 580, 620, 630, 1016, 1082
Alw21I GWGCWC 1 cut(s) 572
Alw26I GTCTC 2 cut(s) 324, 455
AlwI GGATC 2 cut(s) 247, 521
AlwNI CAGNNNCTG 2 cut(s) 329, 500
Ama87I CYCGRG 2 cut(s) 7, 837
AoxI GGCC 3 cut(s) 3, 206, 298
ApeKI GCWGC 5 cut(s) 220, 247, 580, 821, 937
ApoI RAATTY 1 cut(s) 1069
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 2 cut(s) 132, 259
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 3 cut(s) 350, 389, 543
AvaI CYCGRG 2 cut(s) 7, 837
AxyI CCTNAGG 1 cut(s) 330
BalI TGGCCA 1 cut(s) 300
BanI GGYRCC 1 cut(s) 256
BbsI GAAGAC 2 cut(s) 96, 152
Bbv12I GWGCWC 1 cut(s) 572
BbvI GCAGC 5 cut(s) 232, 259, 567, 808, 949
BccI CCATC 5 cut(s) 341, 445, 556, 875, 1070
BceAI ACGGC 4 cut(s) 172, 183, 193, 218
BciVI GTATCC 1 cut(s) 917
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 324, 455
BfaI CTAG 1 cut(s) 972
BfmI CTRYAG 3 cut(s) 218, 492, 661
BfoI RGCGCY 1 cut(s) 260
BfuI GTATCC 1 cut(s) 917
BglII AGATCT 1 cut(s) 968
Bme1390I CCNGG 1 cut(s) 261
BmeT110I CYCGRG 2 cut(s) 7, 837
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 4 cut(s) 27, 39, 165, 186
BpiI GAAGAC 2 cut(s) 96, 152
BpmI CTGGAG 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 2 cut(s) 99, 257
BsaJI CCNNGG 2 cut(s) 260, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 842
Bse1I ACTGG 1 cut(s) 922
Bse21I CCTNAGG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 708
BseDI CCNNGG 2 cut(s) 260, 836
BseGI GGATG 2 cut(s) 195, 831
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 2 cut(s) 335, 842
BseMII CTCAG 6 cut(s) 242, 321, 413, 423, 585, 1100
BseNI ACTGG 1 cut(s) 922
BseRI GAGGAG 4 cut(s) 347, 411, 590, 974
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 5 cut(s) 232, 259, 567, 808, 949
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 3 cut(s) 35, 114, 132
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 3 cut(s) 5, 208, 300
BshNI GGYRCC 1 cut(s) 256
BsiEI CGRYCG 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 572
BsiHKCI CYCGRG 2 cut(s) 7, 837
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 3 cut(s) 295, 854, 898
BslI CCNNNNNNNGG 2 cut(s) 335, 842
BsmAI GTCTC 2 cut(s) 324, 455
BsmFI GGGAC 3 cut(s) 295, 854, 898
BsnI GGCC 3 cut(s) 5, 208, 300
BsoBI CYCGRG 2 cut(s) 7, 837
Bsp1286I GDGCHC 1 cut(s) 572
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 6 cut(s) 239, 511, 526, 709, 968, 995
Bsp68I TCGCGA 1 cut(s) 35
BspANI GGCC 3 cut(s) 5, 208, 300
BspCNI CTCAG 6 cut(s) 241, 322, 412, 424, 584, 1101
BspFNI CGCG 3 cut(s) 35, 114, 132
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 2 cut(s) 247, 521
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 256
BsrGI TGTACA 1 cut(s) 48
BsrI ACTGG 1 cut(s) 922
BssECI CCNNGG 2 cut(s) 260, 836
BssMI GATC 6 cut(s) 239, 511, 526, 709, 968, 995
BssNI GRCGYC 2 cut(s) 99, 257
Bst4CI ACNGT 2 cut(s) 845, 929
Bst6I CTCTTC 3 cut(s) 75, 120, 959
BstACI GRCGYC 2 cut(s) 99, 257
BstAPI GCANNNNNTGC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 687, 819
BstDEI CTNAG 6 cut(s) 228, 330, 399, 432, 571, 1109
BstF5I GGATG 2 cut(s) 195, 831
BstFNI CGCG 3 cut(s) 35, 114, 132
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 2 cut(s) 132, 259
BstKTI GATC 6 cut(s) 242, 514, 529, 712, 971, 998
BstMAI GTCTC 2 cut(s) 324, 455
BstMBI GATC 6 cut(s) 239, 511, 526, 709, 968, 995
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 4 cut(s) 205, 256, 497, 691
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 3 cut(s) 218, 492, 661
BstUI CGCG 3 cut(s) 35, 114, 132
BstV1I GCAGC 5 cut(s) 232, 259, 567, 808, 949
BstV2I GAAGAC 2 cut(s) 96, 152
BstX2I RGATCY 1 cut(s) 968
BstYI RGATCY 1 cut(s) 968
BstZI CGGCCG 1 cut(s) 206
Bsu36I CCTNAGG 1 cut(s) 330
BsuI GTATCC 1 cut(s) 917
BsuRI GGCC 3 cut(s) 5, 208, 300
BtgZI GCGATG 3 cut(s) 16, 50, 188
BtsCI GGATG 2 cut(s) 195, 831
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 2 cut(s) 452, 696
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 687, 819
CaiI CAGNNNCTG 2 cut(s) 329, 500
CfoI GCGC 2 cut(s) 132, 259
CseI GACGC 1 cut(s) 292
Csp6I GTAC 1 cut(s) 49
CviAII CATG 8 cut(s) 94, 302, 316, 386, 686, 723, 752, 890
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 6 cut(s) 228, 330, 399, 432, 571, 1109
DinI GGCGCC 1 cut(s) 258
DpnI GATC 6 cut(s) 241, 513, 528, 711, 970, 997
DpnII GATC 6 cut(s) 239, 511, 526, 709, 968, 995
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 2 cut(s) 206, 298
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 3 cut(s) 75, 120, 959
EarI CTCTTC 3 cut(s) 75, 120, 959
EclXI CGGCCG 1 cut(s) 206
Eco32I GATATC 1 cut(s) 829
Eco52I CGGCCG 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 126, 712
Eco81I CCTNAGG 1 cut(s) 330
Eco88I CYCGRG 2 cut(s) 7, 837
EcoRV GATATC 1 cut(s) 829
EgeI GGCGCC 1 cut(s) 258
EheI GGCGCC 1 cut(s) 258
FaeI CATG 8 cut(s) 97, 305, 319, 389, 689, 726, 755, 893
FaqI GGGAC 3 cut(s) 295, 854, 898
FatI CATG 8 cut(s) 93, 301, 315, 385, 685, 722, 751, 889
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 1 cut(s) 709
FblI GTMKAC 2 cut(s) 368, 847
FokI GGATG 2 cut(s) 202, 818
FspBI CTAG 1 cut(s) 972
GlaI GCGC 2 cut(s) 131, 258
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 260
HaeIII GGCC 3 cut(s) 5, 208, 300
HapII CCGG 1 cut(s) 260
HgaI GACGC 1 cut(s) 292
HhaI GCGC 2 cut(s) 132, 259
Hin1I GRCGYC 2 cut(s) 99, 257
Hin1II CATG 8 cut(s) 97, 305, 319, 389, 689, 726, 755, 893
Hin6I GCGC 2 cut(s) 130, 257
HinP1I GCGC 2 cut(s) 130, 257
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 7 cut(s) 214, 380, 463, 473, 592, 667, 886
HpaII CCGG 1 cut(s) 260
HphI GGTGA 3 cut(s) 350, 389, 543
Hpy166II GTNNAC 3 cut(s) 292, 369, 848
Hpy188I TCNGA 7 cut(s) 433, 448, 478, 574, 714, 1075, 1110
Hpy188III TCNNGA 7 cut(s) 9, 34, 323, 605, 671, 701, 839
Hpy8I GTNNAC 3 cut(s) 292, 369, 848
Hpy99I CGWCG 3 cut(s) 59, 104, 238
HpyAV CCTTC 2 cut(s) 649, 994
HpyCH4III ACNGT 2 cut(s) 845, 929
HpyCH4IV ACGT 2 cut(s) 99, 184
HpyCH4V TGCA 7 cut(s) 199, 220, 506, 583, 685, 689, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 256, 497, 691
HpyF3I CTNAG 6 cut(s) 228, 330, 399, 432, 571, 1109
HpySE526I ACGT 2 cut(s) 99, 184
Hsp92I GRCGYC 2 cut(s) 99, 257
Hsp92II CATG 8 cut(s) 97, 305, 319, 389, 689, 726, 755, 893
HspAI GCGC 2 cut(s) 130, 257
KasI GGCGCC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 6 cut(s) 239, 511, 526, 709, 968, 995
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 2 cut(s) 577, 1079
Lsp1109I GCAGC 5 cut(s) 232, 259, 567, 808, 949
LweI GCATC 4 cut(s) 27, 39, 165, 186
MaeI CTAG 1 cut(s) 972
MaeII ACGT 2 cut(s) 99, 184
MaeIII GTNAC 3 cut(s) 415, 767, 1008
MalI GATC 6 cut(s) 241, 513, 528, 711, 970, 997
MboI GATC 6 cut(s) 239, 511, 526, 709, 968, 995
MflI RGATCY 1 cut(s) 968
MhlI GDGCHC 1 cut(s) 572
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 5 cut(s) 20, 342, 608, 1019, 1069
MluNI TGGCCA 1 cut(s) 300
Mly113I GGCGCC 1 cut(s) 257
MlyI GAGTC 2 cut(s) 457, 880
MmeI TCCRAC 1 cut(s) 167
Mox20I TGGCCA 1 cut(s) 300
MroXI GAANNNNTTC 1 cut(s) 629
MscI TGGCCA 1 cut(s) 300
MseI TTAA 3 cut(s) 404, 557, 611
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 300
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 261
MvnI CGCG 3 cut(s) 35, 114, 132
MwoI GCNNNNNNNGC 4 cut(s) 205, 256, 497, 691
NarI GGCGCC 1 cut(s) 257
NciI CCSGG 1 cut(s) 261
NdeII GATC 6 cut(s) 239, 511, 526, 709, 968, 995
NlaIII CATG 8 cut(s) 97, 305, 319, 389, 689, 726, 755, 893
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 767
NruI TCGCGA 1 cut(s) 35
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 5 cut(s) 214, 380, 473, 592, 667
PleI GAGTC 2 cut(s) 457, 880
PluTI GGCGCC 1 cut(s) 260
PpsI GAGTC 2 cut(s) 457, 880
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 2 cut(s) 329, 500
PsuI RGATCY 1 cut(s) 968
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 404, 557, 611
Sau3AI GATC 6 cut(s) 239, 511, 526, 709, 968, 995
SchI GAGTC 2 cut(s) 457, 880
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 572
SfaNI GCATC 4 cut(s) 27, 39, 165, 186
SfcI CTRYAG 3 cut(s) 218, 492, 661
SfoI GGCGCC 1 cut(s) 258
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 7, 563
SmoI CTYRAG 2 cut(s) 7, 563
SphI GCATGC 1 cut(s) 689
Sse9I AATT 5 cut(s) 20, 342, 608, 1019, 1069
SspDI GGCGCC 1 cut(s) 256
SspMI CTAG 1 cut(s) 972
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 845, 929
TaiI ACGT 2 cut(s) 102, 187
TaqI TCGA 7 cut(s) 8, 24, 44, 194, 744, 863, 998
TasI AATT 5 cut(s) 20, 342, 608, 1019, 1069
TatI WGTACW 1 cut(s) 48
TauI GCSGC 6 cut(s) 19, 117, 135, 173, 208, 247
TfiI GAWTC 5 cut(s) 214, 380, 473, 592, 667
Tru1I TTAA 3 cut(s) 404, 557, 611
Tru9I TTAA 3 cut(s) 404, 557, 611
TscAI CASTG 2 cut(s) 459, 696
TseFI GTSAC 1 cut(s) 767
TseI GCWGC 5 cut(s) 220, 247, 580, 821, 937
Tsp45I GTSAC 1 cut(s) 767
TspDTI ATGAA 4 cut(s) 321, 651, 711, 795
TspGWI ACGGA 2 cut(s) 164, 961
TspRI CASTG 2 cut(s) 459, 696
XapI RAATTY 1 cut(s) 1069
XceI RCATGY 1 cut(s) 689
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 368, 847
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 1 cut(s) 972
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.