FvH4_3g37800

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008773
Physical Location & Seq
Reverse (-)
32369096 .. 32373816
4721 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37800.t2

Sequence Viewer

Length: 1071 bp
ATGGCCTCAAGAAAGTTCGAGGGCATCGCGATGCTGTCAATGTACGACGACGACGACATGGACGACGCTGAAGAACACGGAAATCAAGAAAACCACGGCGGCGGCGATGCGTCCGTGCTGGTTGTGGATGCAACGGCGGCCGTTGATTCTGGTAACGACGATAATGCAACGAGCGGTTTGGATGATGAGAACTTGTCCCGGAGTAAACAGCAGCTGTCTCAGGCTGTGGCGCTGGAGAAGTTGAGTCTGAGAGGGACGCTTGTGGACTATGGCCATGATGAAGTTGCTATGTCTCCAGAGCCTCAGGAGGGTGAAATTGATGGAGGTGGACGCATTGTGTCTGCGAACGGTGATTCACGCGATAAGACACCGCAGGGAACTCTTCAAATATCTGAGCCATCACAATCTGATGCAATGAATGGGACTGTGCTTGAATCAGATGGTAGAAATACTACAGAGGCTGTTGCAGAGGATAAGAAAGTTGATCCGTTAGATATTTTTCTTCCACCACCTTTAAAGCTCAAGTGCTCAGAGGAGCTGCAAAGAAGGATTCAGAAGTTTCTTGAATTAAAGAGAGCAGGGAAAAGCTTCAATGAAGAAGTGCGGAAAAAGAAAGACTATAGGAACCCAGACTTTCTATTGCATGCGGTGCGGTATCAAGATATTGATCAAATAGGTTCATGCTTCAATAAAGATGTATTTGACCCTCATGGATTTGACAAAAGCGACTACTATGATGAAATAGAGGCTGATTCTAGACGTGAACTGGAGAGGATTAATCAAGAAAAAAAGAATCGTCAGAATCATGAATTTGTTTCTGGTGGAAGACAATCTACAGTAGTTGGTGCTGCACCTAAACTTAACGTACCTCTCTCAGGTATAACCATGGATGCTACCGGGTTGAACTCCTTGCCACCTGCTGCTGATATCATCCCTCGGGACGGTAGACAGAATAAGAAGACGAAGTGGGATAAGATAGATGGGACTCATGCAACACTATTATCTTCTGCTAATGCTGGTGGATACACTTTGGGGCATCAGAAACAAAGAGAGGTGGAGGAGGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

357

Amino Acids

39.2

Weight (kDa)

4.77

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 168 - 258 9.2e-28 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 925
Acc36I ACCTGC 1 cut(s) 925
AccBSI CCGCTC 1 cut(s) 174
AccI GTMKAC 1 cut(s) 946
AccII CGCG 2 cut(s) 29, 360
AciI CCGC 8 cut(s) 99, 102, 137, 174, 371, 604, 647, 652
AclWI GGATC 1 cut(s) 479
AcoI YGGCCR 2 cut(s) 138, 271
AcsI RAATTY 1 cut(s) 809
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 2 cut(s) 44, 867
AfiI CCNNNNNNNGG 3 cut(s) 308, 875, 941
AgsI TTSAA 6 cut(s) 386, 434, 566, 592, 688, 904
AjiI CACGTC 1 cut(s) 761
AluBI AGCT 4 cut(s) 214, 520, 538, 588
AluI AGCT 4 cut(s) 214, 520, 538, 588
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 2 cut(s) 222, 297
AlwI GGATC 1 cut(s) 479
AlwNI CAGNNNCTG 2 cut(s) 214, 461
Ama87I CYCGRG 1 cut(s) 936
AoxI GGCC 3 cut(s) 3, 138, 271
ApeKI GCWGC 4 cut(s) 211, 538, 848, 920
ApoI RAATTY 1 cut(s) 809
AseI ATTAAT 1 cut(s) 777
Asp700I GAANNNNTTC 1 cut(s) 587
AspLEI GCGC 1 cut(s) 232
AsuC2I CCSGG 2 cut(s) 199, 898
AsuHPI GGTGA 2 cut(s) 323, 362
AvaI CYCGRG 1 cut(s) 936
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 817, 849
BbsI GAAGAC 2 cut(s) 832, 965
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 223, 525, 835, 907
BccI CCATC 4 cut(s) 314, 406, 434, 974
BceAI ACGGC 3 cut(s) 112, 125, 150
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciVI GTATCC 1 cut(s) 1016
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 2 cut(s) 199, 898
BcoDI GTCTC 2 cut(s) 222, 297
BfaI CTAG 2 cut(s) 756, 1069
BfmI CTRYAG 3 cut(s) 453, 619, 834
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 925
BfuI GTATCC 1 cut(s) 1016
BglII AGATCT 1 cut(s) 1065
BisI GCNGC 7 cut(s) 100, 103, 138, 212, 539, 849, 921
BlsI GCNGC 7 cut(s) 101, 104, 139, 213, 540, 850, 922
Bme1390I CCNGG 2 cut(s) 199, 898
BmeT110I CYCGRG 1 cut(s) 936
BmgBI CACGTC 1 cut(s) 761
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 2 cut(s) 199, 898
BmsI GCATC 7 cut(s) 21, 33, 97, 118, 400, 880, 1045
BpiI GAAGAC 2 cut(s) 832, 965
BpmI CTGGAG 3 cut(s) 254, 279, 788
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 2 cut(s) 199, 898
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 94, 885, 935
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 875, 941
Bse1I ACTGG 1 cut(s) 771
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 666
BseDI CCNNGG 3 cut(s) 94, 885, 935
BseGI GGATG 4 cut(s) 133, 187, 895, 930
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 308, 875, 941
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 6 cut(s) 233, 239, 317, 384, 543, 888
BseNI ACTGG 1 cut(s) 771
BseRI GAGGAG 1 cut(s) 548
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 223, 525, 835, 907
BsgI GTGCAG 1 cut(s) 834
Bsh1236I CGCG 2 cut(s) 29, 360
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 5, 140, 273
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 936
BsiSI CCGG 2 cut(s) 199, 897
BslFI GGGAC 5 cut(s) 181, 268, 436, 953, 997
BslI CCNNNNNNNGG 3 cut(s) 308, 875, 941
BsmAI GTCTC 2 cut(s) 222, 297
BsmFI GGGAC 5 cut(s) 181, 268, 436, 953, 997
BsnI GGCC 3 cut(s) 5, 140, 273
BsoBI CYCGRG 1 cut(s) 936
Bsp1286I GDGCHC 1 cut(s) 530
Bsp143I GATC 3 cut(s) 484, 667, 1065
Bsp19I CCATGG 1 cut(s) 885
Bsp68I TCGCGA 1 cut(s) 29
BspACI CCGC 8 cut(s) 99, 102, 137, 174, 371, 604, 647, 652
BspANI GGCC 3 cut(s) 5, 140, 273
BspCNI CTCAG 6 cut(s) 232, 240, 316, 385, 542, 887
BspFNI CGCG 2 cut(s) 29, 360
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 1 cut(s) 626
BspMI ACCTGC 1 cut(s) 925
BspPI GGATC 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 420
BsrI ACTGG 1 cut(s) 771
BssECI CCNNGG 3 cut(s) 94, 885, 935
BssMI GATC 3 cut(s) 484, 667, 1065
BssT1I CCWWGG 1 cut(s) 885
Bst4CI ACNGT 4 cut(s) 350, 427, 838, 944
Bst6I CTCTTC 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 649
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 6 cut(s) 219, 248, 303, 393, 529, 874
BstDSI CCRYGG 2 cut(s) 94, 885
BstENI CCTNNNNNAGG 1 cut(s) 873
BstF5I GGATG 4 cut(s) 133, 187, 895, 930
BstFNI CGCG 2 cut(s) 29, 360
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 3 cut(s) 487, 670, 1068
BstMAI GTCTC 2 cut(s) 222, 297
BstMBI GATC 3 cut(s) 484, 667, 1065
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 2 cut(s) 137, 649
BstNSI RCATGY 1 cut(s) 647
BstSCI CCNGG 2 cut(s) 197, 896
BstSFI CTRYAG 3 cut(s) 453, 619, 834
BstUI CGCG 2 cut(s) 29, 360
BstV1I GCAGC 4 cut(s) 223, 525, 835, 907
BstV2I GAAGAC 2 cut(s) 832, 965
BstX2I RGATCY 1 cut(s) 1065
BstYI RGATCY 1 cut(s) 1065
BstZI CGGCCG 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 1016
BsuRI GGCC 3 cut(s) 5, 140, 273
BtgI CCRYGG 2 cut(s) 94, 885
BtgZI GCGATG 3 cut(s) 10, 44, 120
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 4 cut(s) 133, 187, 895, 930
BtuMI TCGCGA 1 cut(s) 29
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 1 cut(s) 645
CaiI CAGNNNCTG 2 cut(s) 214, 461
CciI TCATGA 1 cut(s) 805
CfoI GCGC 1 cut(s) 232
CseI GACGC 4 cut(s) 74, 99, 265, 339
Csp6I GTAC 2 cut(s) 43, 866
CviAII CATG 8 cut(s) 58, 275, 644, 681, 710, 806, 886, 989
CviQI GTAC 2 cut(s) 43, 866
DdeI CTNAG 6 cut(s) 219, 248, 303, 393, 529, 874
DpnI GATC 3 cut(s) 486, 669, 1067
DpnII GATC 3 cut(s) 484, 667, 1065
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 2 cut(s) 138, 271
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 885
Eco32I GATATC 1 cut(s) 928
Eco52I CGGCCG 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 936
EcoNI CCTNNNNNAGG 1 cut(s) 873
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 8 cut(s) 61, 278, 647, 684, 713, 809, 889, 992
FaqI GGGAC 5 cut(s) 181, 268, 436, 953, 997
FatI CATG 8 cut(s) 57, 274, 643, 680, 709, 805, 885, 988
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 946
Fnu4HI GCNGC 7 cut(s) 100, 103, 138, 212, 539, 849, 921
FokI GGATG 4 cut(s) 140, 194, 902, 917
Fsp4HI GCNGC 7 cut(s) 100, 103, 138, 212, 539, 849, 921
FspBI CTAG 2 cut(s) 756, 1069
GlaI GCGC 1 cut(s) 231
GluI GCNGC 7 cut(s) 100, 103, 138, 212, 539, 849, 921
GsuI CTGGAG 3 cut(s) 254, 279, 788
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 5, 140, 273
HapII CCGG 2 cut(s) 199, 897
HgaI GACGC 4 cut(s) 74, 99, 265, 339
HhaI GCGC 1 cut(s) 232
Hin1II CATG 8 cut(s) 61, 278, 647, 684, 713, 809, 889, 992
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 586
HinfI GANTC 9 cut(s) 146, 244, 353, 434, 550, 752, 793, 802, 985
HpaII CCGG 2 cut(s) 199, 897
HphI GGTGA 2 cut(s) 323, 362
Hpy166II GTNNAC 5 cut(s) 206, 265, 329, 764, 947
Hpy188I TCNGA 8 cut(s) 249, 394, 409, 439, 532, 555, 801, 1041
Hpy8I GTNNAC 5 cut(s) 206, 265, 329, 764, 947
Hpy99I CGWCG 5 cut(s) 50, 53, 56, 68, 161
HpyAV CCTTC 1 cut(s) 540
HpyCH4III ACNGT 4 cut(s) 350, 427, 838, 944
HpyCH4IV ACGT 2 cut(s) 760, 864
HpyCH4V TGCA 8 cut(s) 131, 167, 413, 467, 541, 643, 851, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 649
HpyF3I CTNAG 6 cut(s) 219, 248, 303, 393, 529, 874
HpySE526I ACGT 2 cut(s) 760, 864
Hsp92II CATG 8 cut(s) 61, 278, 647, 684, 713, 809, 889, 992
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 3 cut(s) 484, 667, 1065
LmnI GCTCC 1 cut(s) 535
Lsp1109I GCAGC 4 cut(s) 223, 525, 835, 907
LweI GCATC 7 cut(s) 21, 33, 97, 118, 400, 880, 1045
MaeI CTAG 2 cut(s) 756, 1069
MaeII ACGT 2 cut(s) 760, 864
MaeIII GTNAC 1 cut(s) 152
MalI GATC 3 cut(s) 486, 669, 1067
MbiI CCGCTC 1 cut(s) 174
MboI GATC 3 cut(s) 484, 667, 1065
MboII GAAGA 7 cut(s) 83, 374, 494, 608, 837, 970, 996
MflI RGATCY 1 cut(s) 1065
MhlI GDGCHC 1 cut(s) 530
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 3 cut(s) 315, 566, 809
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 2 cut(s) 253, 979
Mox20I TGGCCA 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 587
MscI TGGCCA 1 cut(s) 273
MseI TTAA 4 cut(s) 515, 569, 777, 861
MslI CAYNNNNRTG 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 2 cut(s) 199, 897
MspR9I CCNGG 2 cut(s) 199, 898
MvnI CGCG 2 cut(s) 29, 360
MwoI GCNNNNNNNGC 2 cut(s) 137, 649
NciI CCSGG 2 cut(s) 199, 898
NcoI CCATGG 1 cut(s) 885
NdeII GATC 3 cut(s) 484, 667, 1065
NlaIII CATG 8 cut(s) 61, 278, 647, 684, 713, 809, 889, 992
NlaIV GGNNCC 1 cut(s) 626
NruI TCGCGA 1 cut(s) 29
NspI RCATGY 1 cut(s) 647
PaeI GCATGC 1 cut(s) 647
PagI TCATGA 1 cut(s) 805
PaqCI CACCTGC 1 cut(s) 925
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 60, 102
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 7 cut(s) 146, 353, 434, 550, 752, 793, 802
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 7 cut(s) 101, 104, 139, 213, 540, 850, 922
PleI GAGTC 2 cut(s) 252, 979
PpsI GAGTC 2 cut(s) 252, 979
PshBI ATTAAT 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 626
PstNI CAGNNNCTG 2 cut(s) 214, 461
PsuI RGATCY 1 cut(s) 1065
PvuII CAGCTG 1 cut(s) 214
RruI TCGCGA 1 cut(s) 29
RsaI GTAC 2 cut(s) 44, 867
RsaNI GTAC 2 cut(s) 43, 866
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 4 cut(s) 515, 569, 777, 861
SatI GCNGC 7 cut(s) 100, 103, 138, 212, 539, 849, 921
Sau3AI GATC 3 cut(s) 484, 667, 1065
SchI GAGTC 2 cut(s) 253, 979
ScrFI CCNGG 2 cut(s) 199, 898
SduI GDGCHC 1 cut(s) 530
SfaNI GCATC 7 cut(s) 21, 33, 97, 118, 400, 880, 1045
SfcI CTRYAG 3 cut(s) 453, 619, 834
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 7, 521
SmoI CTYRAG 2 cut(s) 7, 521
SphI GCATGC 1 cut(s) 647
Sse9I AATT 3 cut(s) 315, 566, 809
SsiI CCGC 8 cut(s) 99, 102, 137, 174, 371, 604, 647, 652
SspMI CTAG 2 cut(s) 756, 1069
StyD4I CCNGG 2 cut(s) 197, 896
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 4 cut(s) 350, 427, 838, 944
TaiI ACGT 2 cut(s) 763, 867
TaqI TCGA 1 cut(s) 18
TasI AATT 3 cut(s) 315, 566, 809
TauI GCSGC 3 cut(s) 102, 105, 140
TfiI GAWTC 7 cut(s) 146, 353, 434, 550, 752, 793, 802
Tru1I TTAA 4 cut(s) 515, 569, 777, 861
Tru9I TTAA 4 cut(s) 515, 569, 777, 861
TseI GCWGC 4 cut(s) 211, 538, 848, 920
TspDTI ATGAA 6 cut(s) 294, 431, 609, 669, 753, 822
TspGWI ACGGA 3 cut(s) 93, 103, 477
VspI ATTAAT 1 cut(s) 777
XagI CCTNNNNNAGG 1 cut(s) 873
XapI RAATTY 1 cut(s) 809
XbaI TCTAGA 1 cut(s) 755
XceI RCATGY 1 cut(s) 647
XmiI GTMKAC 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 2 cut(s) 756, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.