FvH4_3g08180

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008848
Physical Location & Seq
Reverse (-)
4806272 .. 4808084
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08180.t1

Sequence Viewer

Length: 1248 bp
ATGGCTACAATGAACATGAGCATGGTTGCAGGGTCAGGAATCATGCTTCTTACACTAGTATGGGGTTCTGTTGTAGTATTTGGCAGCTCTGATGTCTCGGAGTCTCCCGGTTCTTTAAATCAAGGACTTAAGAAGTTCTTCAGCACTTGTTATTGTGTAACAACTGATGCTGAAACCCGCTCTACTGCAAGAATCATGATGGTGTCAGTGATCCCCTTTCTCATTCTTCAAGTGGCTAAAGTCCTTTCTTCAACCTCCGGGATTCGTATCGTTATCCTAGTTTCTCTACTTGTTTCAATTGCATTACTAGTCACTTACTGCATCTATCAGGTCTTTACACCATGGATTCAGGACAGAAGACTTGATTTTTTGTTACCCGGTTTTCTCAATGCAAACGGAGACCCTAATGTATCATATATACTAGAGGTGTTCAGTAGAATCGACCAGAATCACAATAAATATATATCAGAATATGAACTGAGAGCATTGATCCTAGGAATACAGATAAGAGATGTAGGCTTGGAGATTGATTATCTAGAGAAGGCAATGAAGAACTTTGATAAATCCGGTGATTCTCGAATATCTGAGAAGGAGTTTGTTGATGGAATCTCAAAATGGGTTAATGCAACGCCCTCTGCCAATGGTCAAGGTCAAGGTCAAGCTCAAGGACAAAGAAGGTTTCTAAGCAGCAAGTCCAAGGAAGCTTTGGACGAGCAGCAGAAGCAGGTTCCTAAGAAAATGAAGAATAAGCATATGGATAAAACTTGGAGGAATCAACTTAAGGCTGCTTGTTTTTTGATCATCGGGATTGGCATTATGATCTTGCTTGCAACACCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTGTCCTCCTATGTTTTGATACCCTTCGCTATCAACATTGGACAGGCATTGCAACTTGTAACTTCTGCGCAACACAAGACTGAGAATGCCATCTCTTTGACACTCTCAGAGATTTATAATGGCGTTTTCATGAACAACATTATGGGATTGACCATCTTCTTGGCACTTGTTTACATACGTAACCTATCATGGGATGTCGGTGCAGCATCCCTAGCTGTTTTGATCATTTGCTCAGTAATGGGTGTTTATACCAGCTTCAGCACAAAGTTCCCATTTTGGACATGTATTTTAGCCTACATTATGTACCCCATATCACTGCTCATGATTTATGTTCTTACATCTTATTTTGGATGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

416

Amino Acids

46.14

Weight (kDa)

8.6

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 139 - 203 9.4e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc16I TGCGCA 1 cut(s) 957
Acc36I ACCTGC 1 cut(s) 715
AccBSI CCGCTC 1 cut(s) 180
AciI CCGC 1 cut(s) 178
AclWI GGATC 2 cut(s) 205, 484
AcuI CTGAAG 2 cut(s) 124, 1129
AfaI GTAC 1 cut(s) 1193
AfiI CCNNNNNNNGG 1 cut(s) 1078
AflII CTTAAG 2 cut(s) 128, 779
AflIII ACRYGT 1 cut(s) 1169
AgsI TTSAA 3 cut(s) 230, 252, 297
AhlI ACTAGT 2 cut(s) 55, 307
AluBI AGCT 5 cut(s) 87, 662, 704, 1103, 1143
AluI AGCT 5 cut(s) 87, 662, 704, 1103, 1143
Alw26I GTCTC 4 cut(s) 100, 108, 393, 839
AlwI GGATC 2 cut(s) 205, 484
ApeKI GCWGC 6 cut(s) 84, 687, 715, 785, 869, 1091
Asp700I GAANNNNTTC 1 cut(s) 137
AspA2I CCTAGG 1 cut(s) 493
AspLEI GCGC 1 cut(s) 958
AsuC2I CCSGG 3 cut(s) 108, 259, 378
AsuHPI GGTGA 1 cut(s) 581
AvrII CCTAGG 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 364
BbvI GCAGC 6 cut(s) 96, 699, 727, 772, 856, 1103
BccI CCATC 5 cut(s) 193, 596, 986, 1049, 1233
BclI TGATCA 2 cut(s) 798, 1110
BcnI CCSGG 3 cut(s) 108, 259, 378
BcoDI GTCTC 4 cut(s) 100, 108, 393, 839
BcuI ACTAGT 2 cut(s) 55, 307
BfaI CTAG 7 cut(s) 56, 278, 308, 422, 494, 536, 1100
BfrI CTTAAG 2 cut(s) 128, 779
BfuAI ACCTGC 1 cut(s) 715
BisI GCNGC 6 cut(s) 85, 688, 716, 786, 870, 1092
BlnI CCTAGG 1 cut(s) 493
BlsI GCNGC 6 cut(s) 86, 689, 717, 787, 871, 1093
Bme1390I CCNGG 3 cut(s) 108, 259, 378
BmiI GGNNCC 1 cut(s) 729
BmrFI CCNGG 3 cut(s) 108, 259, 378
BmsI GCATC 4 cut(s) 157, 330, 885, 1103
BpiI GAAGAC 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 648
BpuMI CCSGG 3 cut(s) 108, 259, 378
BsaAI YACGTR 1 cut(s) 1067
BsaBI GATNNNNATC 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 341, 493, 696
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 1 cut(s) 1078
Bse3DI GCAATG 2 cut(s) 552, 935
Bse8I GATNNNNATC 1 cut(s) 266
BseDI CCNNGG 3 cut(s) 341, 493, 696
BseGI GGATG 4 cut(s) 876, 1087, 1094, 1244
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 1 cut(s) 1078
BseMI GCAATG 2 cut(s) 552, 935
BseMII CTCAG 5 cut(s) 470, 576, 960, 1008, 1134
BseXI GCAGC 6 cut(s) 96, 699, 727, 772, 856, 1103
BsgI GTGCAG 1 cut(s) 1110
BsiSI CCGG 4 cut(s) 108, 258, 378, 567
BslI CCNNNNNNNGG 1 cut(s) 1078
BsmAI GTCTC 4 cut(s) 100, 108, 393, 839
BsmI GAATGC 1 cut(s) 979
Bso31I GGTCTC 1 cut(s) 393
Bsp143I GATC 5 cut(s) 210, 489, 798, 819, 1110
Bsp19I CCATGG 1 cut(s) 341
BspACI CCGC 1 cut(s) 178
BspCNI CTCAG 5 cut(s) 471, 577, 961, 1007, 1133
BspHI TCATGA 3 cut(s) 195, 1017, 1209
BspLI GGNNCC 1 cut(s) 729
BspMI ACCTGC 1 cut(s) 715
BspPI GGATC 2 cut(s) 205, 484
BspTI CTTAAG 2 cut(s) 128, 779
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 2 cut(s) 552, 935
BssECI CCNNGG 3 cut(s) 341, 493, 696
BssMI GATC 5 cut(s) 210, 489, 798, 819, 1110
BssT1I CCWWGG 3 cut(s) 341, 493, 696
BstAFI CTTAAG 2 cut(s) 128, 779
BstBAI YACGTR 1 cut(s) 1067
BstC8I GCNNGC 2 cut(s) 828, 874
BstDEI CTNAG 7 cut(s) 479, 585, 683, 732, 969, 994, 1120
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 4 cut(s) 876, 1087, 1094, 1244
BstHHI GCGC 1 cut(s) 958
BstKTI GATC 5 cut(s) 213, 492, 801, 822, 1113
BstMAI GTCTC 4 cut(s) 100, 108, 393, 839
BstMBI GATC 5 cut(s) 210, 489, 798, 819, 1110
BstMWI GCNNNNNNNGC 2 cut(s) 721, 1100
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 3 cut(s) 106, 257, 376
BstSNI TACGTA 1 cut(s) 1067
BstV1I GCAGC 6 cut(s) 96, 699, 727, 772, 856, 1103
BstV2I GAAGAC 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 1048
BtgI CCRYGG 1 cut(s) 341
BtsCI GGATG 4 cut(s) 876, 1087, 1094, 1244
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 2 cut(s) 213, 1202
BveI ACCTGC 1 cut(s) 715
Cac8I GCNNGC 2 cut(s) 828, 874
CciI TCATGA 3 cut(s) 195, 1017, 1209
CfoI GCGC 1 cut(s) 958
Csp6I GTAC 1 cut(s) 1192
CviQI GTAC 1 cut(s) 1192
DdeI CTNAG 7 cut(s) 479, 585, 683, 732, 969, 994, 1120
DpnI GATC 5 cut(s) 212, 491, 800, 821, 1112
DpnII GATC 5 cut(s) 210, 489, 798, 819, 1110
DraI TTTAAA 1 cut(s) 117
Eco105I TACGTA 1 cut(s) 1067
Eco130I CCWWGG 3 cut(s) 341, 493, 696
Eco31I GGTCTC 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 124, 1129
EcoT14I CCWWGG 3 cut(s) 341, 493, 696
ErhI CCWWGG 3 cut(s) 341, 493, 696
FalI AAGNNNNNCTT 4 cut(s) 122, 154, 865, 897
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 753
FbaI TGATCA 2 cut(s) 798, 1110
Fnu4HI GCNGC 6 cut(s) 85, 688, 716, 786, 870, 1092
FokI GGATG 3 cut(s) 863, 1081, 1094
Fsp4HI GCNGC 6 cut(s) 85, 688, 716, 786, 870, 1092
FspBI CTAG 7 cut(s) 56, 278, 308, 422, 494, 536, 1100
FspI TGCGCA 1 cut(s) 957
GlaI GCGC 1 cut(s) 957
GluI GCNGC 6 cut(s) 85, 688, 716, 786, 870, 1092
HapII CCGG 4 cut(s) 108, 258, 378, 567
HhaI GCGC 1 cut(s) 958
Hin6I GCGC 1 cut(s) 956
HinP1I GCGC 1 cut(s) 956
HindIII AAGCTT 1 cut(s) 702
HpaII CCGG 4 cut(s) 108, 258, 378, 567
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 1 cut(s) 1060
Hpy188I TCNGA 5 cut(s) 91, 100, 469, 586, 997
Hpy188III TCNNGA 8 cut(s) 36, 196, 350, 536, 576, 805, 1018, 1210
Hpy8I GTNNAC 1 cut(s) 1060
HpyAV CCTTC 5 cut(s) 535, 583, 669, 891, 922
HpyCH4IV ACGT 1 cut(s) 1066
HpyF10VI GCNNNNNNNGC 2 cut(s) 721, 1100
HpyF3I CTNAG 7 cut(s) 479, 585, 683, 732, 969, 994, 1120
HpySE526I ACGT 1 cut(s) 1066
HspAI GCGC 1 cut(s) 956
Ksp22I TGATCA 2 cut(s) 798, 1110
Kzo9I GATC 5 cut(s) 210, 489, 798, 819, 1110
Lsp1109I GCAGC 6 cut(s) 96, 699, 727, 772, 856, 1103
LweI GCATC 4 cut(s) 157, 330, 885, 1103
MaeI CTAG 7 cut(s) 56, 278, 308, 422, 494, 536, 1100
MaeII ACGT 1 cut(s) 1066
MaeIII GTNAC 5 cut(s) 157, 310, 372, 946, 1067
MalI GATC 5 cut(s) 212, 491, 800, 821, 1112
MbiI CCGCTC 1 cut(s) 180
MboI GATC 5 cut(s) 210, 489, 798, 819, 1110
MboII GAAGA 7 cut(s) 130, 218, 240, 369, 562, 754, 1036
MfeI CAATTG 1 cut(s) 297
MluCI AATT 1 cut(s) 297
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 6 cut(s) 265, 418, 643, 762, 848, 904
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 5 cut(s) 116, 129, 621, 780, 1246
MslI CAYNNNNRTG 3 cut(s) 20, 58, 200
MspCI CTTAAG 2 cut(s) 128, 779
MspI CCGG 4 cut(s) 108, 258, 378, 567
MspR9I CCNGG 3 cut(s) 108, 259, 378
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 979
MwoI GCNNNNNNNGC 2 cut(s) 721, 1100
NciI CCSGG 3 cut(s) 108, 259, 378
NcoI CCATGG 1 cut(s) 341
NdeI CATATG 1 cut(s) 753
NdeII GATC 5 cut(s) 210, 489, 798, 819, 1110
NlaIV GGNNCC 1 cut(s) 729
NmuCI GTSAC 1 cut(s) 310
NsbI TGCGCA 1 cut(s) 957
NspI RCATGY 1 cut(s) 1173
PagI TCATGA 3 cut(s) 195, 1017, 1209
PciI ACATGT 1 cut(s) 1169
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 9 cut(s) 39, 192, 262, 346, 438, 448, 572, 606, 772
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 6 cut(s) 86, 689, 717, 787, 871, 1093
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
Ppu21I YACGTR 1 cut(s) 1067
PscI ACATGT 1 cut(s) 1169
PsiI TTATAA 1 cut(s) 1005
PspN4I GGNNCC 1 cut(s) 729
RsaI GTAC 1 cut(s) 1193
RsaNI GTAC 1 cut(s) 1192
RseI CAYNNNNRTG 3 cut(s) 20, 58, 200
SaqAI TTAA 5 cut(s) 116, 129, 621, 780, 1246
SatI GCNGC 6 cut(s) 85, 688, 716, 786, 870, 1092
Sau3AI GATC 5 cut(s) 210, 489, 798, 819, 1110
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 108, 259, 378
SfaNI GCATC 4 cut(s) 157, 330, 885, 1103
SmiMI CAYNNNNRTG 3 cut(s) 20, 58, 200
SmlI CTYRAG 3 cut(s) 128, 663, 779
SmoI CTYRAG 3 cut(s) 128, 663, 779
SnaBI TACGTA 1 cut(s) 1067
SpeI ACTAGT 2 cut(s) 55, 307
Sse9I AATT 1 cut(s) 297
SsiI CCGC 1 cut(s) 178
SspMI CTAG 7 cut(s) 56, 278, 308, 422, 494, 536, 1100
StyD4I CCNGG 3 cut(s) 106, 257, 376
StyI CCWWGG 3 cut(s) 341, 493, 696
TaiI ACGT 1 cut(s) 1069
TaqI TCGA 2 cut(s) 441, 577
TasI AATT 1 cut(s) 297
TfiI GAWTC 9 cut(s) 39, 192, 262, 346, 438, 448, 572, 606, 772
Tru1I TTAA 5 cut(s) 116, 129, 621, 780, 1246
Tru9I TTAA 5 cut(s) 116, 129, 621, 780, 1246
TscAI CASTG 2 cut(s) 213, 1209
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 6 cut(s) 84, 687, 715, 785, 869, 1091
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 7 cut(s) 26, 489, 563, 755, 873, 1006, 1034
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 2 cut(s) 213, 1209
Vha464I CTTAAG 2 cut(s) 128, 779
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 1173
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmaJI CCTAGG 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 7 cut(s) 56, 278, 308, 422, 494, 536, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.