MD10G1284900.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008848
Physical Location & Seq
Reverse (-)
37491522 .. 37494617
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1284900.v1.1.491

Sequence Viewer

Length: 1779 bp
ATGTCAAGACCAGCTCTGTTCATCATTTTTCTGGTCCTCCAACTGGTCCATTTAGGAAGCGGCCGGATTGTTAGAGACTCCGGTCTTGTATCCAATGGAATAAACGATGAAATAAGCCAATCTTCAATCCTCGAAGGACTGAATCTTAAATCAGATTCTGTGACTTGTGAACCAATTTTTGGCTTCTTGCCTTGTGCAACTTCAGTTTGGGGGCTTCTTTTTCTAGTAATCATCTACGAGGTCTTGCTCTCCCTCGCAGATCAGTACGTTTCAAGAGGATCTGAAATCTTCTTTGAATTGTTTGGGACTGGTGTCTTTGGTGCTAGTGCTTTCCACCTGCTTGGCTTGATTCCACAAGTTGGAATGGTTCTTGTAATTGGAGTTACAGCAACTACAGAAACTGTGGAGGAAATGGCAGAAATGAGCATGAGCATGCTTGCAGGATCATCAATCATGATTCTTACCCTAATTTGGGGTTCTGTTGTTGCTTTTGGTAGCTCCGACCTCTCGGAATCTCAAACTTTATCAAATCCAGAAAATAAGAAACCCTTAACTGGTTTACTTGCTGTTGCTTTCGGTAGCTCTGACCTCTCGGATTCTCAAACTTCATCAAATCCAGAAAATACGAAACCCTTCAGTTTAACTGGTTCTGGTGTAAGAACTGATGTCGAAACGCGAGATACTGCAAGAATCATGATGTTCTCGTTGATCCCATATCTCATTCTTCAGCTGGCAAAAATCTTGACTTCAACCACAGCGGTTCGAGTTGTGATCTTAATTGCTCTACTGATTGCATCTGCATTCCTTGCCACTTACTTCATTTACCAGTTCTTTCAACCATGGATTCAGAGTAGAAGGCTTGAATATTTGCTACGTAAGTACATACCGAAAAACATACTGCCGAGTCTTATGACAGTAGGCGGAAACCCTAATGTATCTATTATAAAAGGGCTGTTCCATAGAATAGACAAGAATCATAACAAATACATATCATCAGATGAACTTAGAGCATTGATCCTAGGAATACAAATAGAAGAAATAGGTCTGGATGACGATGATTATGTATCAGAAGTGATGAAGCAATTTGATCTCTCTGGCGACGCTCAAATAGCTGAGACGGAGTTTGTGAATGGAATCTCAAAATGGATTAATCCAGCCAAGCCCCCTGCCAACGATAAAGGTCATGAACATACATCGCTTTTCAGAAGAAATAATTCCAAGAATGAGAAATCTATGGAAGATCAGCTTACGCTGCTGATCTCTAAGAAAAATGGGACTAAGGGTGCTGATACATCGTGGATGAATCTCCTGAAGGCTGCTTTTCTAATTATTCTAGGAACTGCCATAACGGTTTTTCTTTCAATGCCCCTCATGGTAAGTCTCCGAAAATTTTCCACTGCTGCAAGCATCCCTTCTTTCGCGGCCTCGTATGTTGTGATACCCTTGGCTACAAACTACAGACTGGCGCTAATGTCAGTAACGTCTGCCAAAGAGAAGACGGAGGATGCCATCTCTTTAACACTTTCTGAGATTTACACAGCGGCGTTCATGAACAACATTGTGGGGTTGGTCATATTTTTGGCGCTCGTTTACATACGAAATTTATCATGGGACGTGGCTGCTGAAGTTTTAGTTGTTTTACTTATTTCGACAGCGGTAGGTTTTTCTGCCAGCTTCAGCAGAAAGTTCCCATTTTGGACATGCATTCTAGCATACATTTTGTACCCAATATCACTGCTGCTGGTTCATGTTCTTACCACTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

593

Amino Acids

64.89

Weight (kDa)

5.64

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 315 - 379 7.8e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AarI CACCTGC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 345
AccB7I CCANNNNNTGG 2 cut(s) 179, 359
AccII CGCG 2 cut(s) 676, 1421
AciI CCGC 6 cut(s) 60, 758, 921, 1421, 1541, 1655
AclWI GGATC 4 cut(s) 286, 451, 703, 1009
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 2 cut(s) 1388, 1600
AcuI CTGAAG 6 cut(s) 186, 619, 710, 1331, 1644, 1660
AfaI GTAC 3 cut(s) 266, 881, 1724
AfiI CCNNNNNNNGG 6 cut(s) 43, 179, 359, 471, 472, 554
AgsI TTSAA 7 cut(s) 126, 273, 296, 750, 836, 863, 1362
AjiI CACGTC 1 cut(s) 1615
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 7 cut(s) 14, 498, 582, 730, 1112, 1246, 1674
AluI AGCT 7 cut(s) 14, 498, 582, 730, 1112, 1246, 1674
Alw26I GTCTC 3 cut(s) 69, 1109, 1385
AlwI GGATC 4 cut(s) 286, 451, 703, 1009
AlwNI CAGNNNCTG 2 cut(s) 158, 401
AoxI GGCC 2 cut(s) 61, 1422
ApeKI GCWGC 5 cut(s) 1252, 1316, 1400, 1619, 1738
ApoI RAATTY 2 cut(s) 1388, 1600
AseI ATTAAT 1 cut(s) 1149
Asp700I GAANNNNTTC 3 cut(s) 632, 1213, 1390
AspA2I CCTAGG 1 cut(s) 1018
AspLEI GCGC 2 cut(s) 1468, 1585
AspS9I GGNCC 2 cut(s) 34, 46
AvaII GGWCC 2 cut(s) 34, 46
AvrII CCTAGG 1 cut(s) 1018
BbsI GAAGAC 1 cut(s) 1502
BbvI GCAGC 5 cut(s) 1239, 1303, 1387, 1606, 1725
BccI CCATC 2 cut(s) 1517, 1764
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 3 cut(s) 69, 1109, 1385
BfaI CTAG 5 cut(s) 224, 324, 1019, 1334, 1709
BfmI CTRYAG 2 cut(s) 393, 1456
BfoI RGCGCY 2 cut(s) 1469, 1586
BfuAI ACCTGC 1 cut(s) 345
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 8 cut(s) 61, 1253, 1317, 1401, 1422, 1542, 1620, 1739
BlnI CCTAGG 1 cut(s) 1018
BlsI GCNGC 8 cut(s) 62, 1254, 1318, 1402, 1423, 1543, 1621, 1740
Bme18I GGWCC 2 cut(s) 34, 46
BmgBI CACGTC 1 cut(s) 1615
BmgT120I GGNCC 2 cut(s) 34, 46
BmsI GCATC 3 cut(s) 803, 1416, 1495
BoxI GACNNNNGTC 2 cut(s) 81, 311
BpiI GAAGAC 1 cut(s) 1502
BsaAI YACGTR 1 cut(s) 875
BsaJI CCNNGG 3 cut(s) 839, 1018, 1443
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 179, 359, 471, 472, 554
Bse1I ACTGG 6 cut(s) 48, 313, 559, 649, 826, 1467
BseDI CCNNGG 3 cut(s) 839, 1018, 1443
BseGI GGATG 5 cut(s) 1054, 1305, 1407, 1510, 1775
BseLI CCNNNNNNNGG 6 cut(s) 43, 179, 359, 471, 472, 554
BseMII CTCAG 2 cut(s) 1104, 1518
BseNI ACTGG 6 cut(s) 48, 313, 559, 649, 826, 1467
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 5 cut(s) 1239, 1303, 1387, 1606, 1725
Bsh1236I CGCG 2 cut(s) 676, 1421
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 63, 1424
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 2 cut(s) 64, 81
BslFI GGGAC 3 cut(s) 319, 1288, 1625
BslI CCNNNNNNNGG 6 cut(s) 43, 179, 359, 471, 472, 554
BsmAI GTCTC 3 cut(s) 69, 1109, 1385
BsmBI CGTCTC 1 cut(s) 1109
BsmFI GGGAC 3 cut(s) 319, 1288, 1625
BsmI GAATGC 2 cut(s) 800, 1704
BsnI GGCC 2 cut(s) 63, 1424
Bsp143I GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 6 cut(s) 60, 758, 921, 1421, 1541, 1655
BspANI GGCC 2 cut(s) 63, 1424
BspCNI CTCAG 2 cut(s) 1105, 1519
BspFNI CGCG 2 cut(s) 676, 1421
BspHI TCATGA 4 cut(s) 453, 693, 1183, 1548
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 4 cut(s) 286, 451, 703, 1009
BsrI ACTGG 6 cut(s) 48, 313, 559, 649, 826, 1467
BssECI CCNNGG 3 cut(s) 839, 1018, 1443
BssMI GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
BssT1I CCWWGG 3 cut(s) 839, 1018, 1443
Bst4CI ACNGT 4 cut(s) 403, 916, 1351, 1762
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 5 cut(s) 434, 438, 732, 1405, 1672
BstDEI CTNAG 5 cut(s) 1004, 1113, 1263, 1278, 1527
BstDSI CCRYGG 1 cut(s) 839
BstF5I GGATG 5 cut(s) 1054, 1305, 1407, 1510, 1775
BstFNI CGCG 2 cut(s) 676, 1421
BstH2I RGCGCY 2 cut(s) 1469, 1586
BstHHI GCGC 2 cut(s) 1468, 1585
BstKTI GATC 9 cut(s) 262, 281, 446, 711, 774, 1017, 1090, 1243, 1260
BstMAI GTCTC 3 cut(s) 69, 1109, 1385
BstMBI GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 3 cut(s) 806, 1109, 1252
BstNSI RCATGY 2 cut(s) 436, 1704
BstPAI GACNNNNGTC 2 cut(s) 81, 311
BstSFI CTRYAG 2 cut(s) 393, 1456
BstSNI TACGTA 1 cut(s) 875
BstUI CGCG 2 cut(s) 676, 1421
BstV1I GCAGC 5 cut(s) 1239, 1303, 1387, 1606, 1725
BstV2I GAAGAC 1 cut(s) 1502
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 61
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 2 cut(s) 63, 1424
BtgI CCRYGG 1 cut(s) 839
BtgZI GCGATG 1 cut(s) 1179
BtrI CACGTC 1 cut(s) 1615
BtsCI GGATG 5 cut(s) 1054, 1305, 1407, 1510, 1775
BtsI GCAGTG 2 cut(s) 1395, 1733
BtsIMutI CAGTG 3 cut(s) 1395, 1733, 1758
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 5 cut(s) 434, 438, 732, 1405, 1672
CaiI CAGNNNCTG 2 cut(s) 158, 401
CciI TCATGA 4 cut(s) 453, 693, 1183, 1548
CfoI GCGC 2 cut(s) 1468, 1585
Cfr13I GGNCC 2 cut(s) 34, 46
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 3 cut(s) 265, 880, 1723
CviQI GTAC 3 cut(s) 265, 880, 1723
DdeI CTNAG 5 cut(s) 1004, 1113, 1263, 1278, 1527
DpnI GATC 9 cut(s) 261, 280, 445, 710, 773, 1016, 1089, 1242, 1259
DpnII GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
EciI GGCGGA 1 cut(s) 936
EclXI CGGCCG 1 cut(s) 61
Eco105I TACGTA 1 cut(s) 875
Eco130I CCWWGG 3 cut(s) 839, 1018, 1443
Eco47I GGWCC 2 cut(s) 34, 46
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 6 cut(s) 186, 619, 710, 1331, 1644, 1660
EcoT14I CCWWGG 3 cut(s) 839, 1018, 1443
EcoT22I ATGCAT 1 cut(s) 1706
ErhI CCWWGG 3 cut(s) 839, 1018, 1443
Esp3I CGTCTC 1 cut(s) 1109
FalI AAGNNNNNCTT 8 cut(s) 106, 138, 533, 565, 1230, 1262, 1396, 1428
FaqI GGGAC 3 cut(s) 319, 1288, 1625
Fnu4HI GCNGC 8 cut(s) 61, 1253, 1317, 1401, 1422, 1542, 1620, 1739
FokI GGATG 4 cut(s) 1061, 1312, 1394, 1517
Fsp4HI GCNGC 8 cut(s) 61, 1253, 1317, 1401, 1422, 1542, 1620, 1739
FspBI CTAG 5 cut(s) 224, 324, 1019, 1334, 1709
GlaI GCGC 2 cut(s) 1467, 1584
GluI GCNGC 8 cut(s) 61, 1253, 1317, 1401, 1422, 1542, 1620, 1739
HaeII RGCGCY 2 cut(s) 1469, 1586
HaeIII GGCC 2 cut(s) 63, 1424
HapII CCGG 2 cut(s) 64, 81
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 2 cut(s) 1468, 1585
Hin6I GCGC 2 cut(s) 1466, 1583
HinP1I GCGC 2 cut(s) 1466, 1583
HpaII CCGG 2 cut(s) 64, 81
Hpy166II GTNNAC 3 cut(s) 170, 560, 1591
Hpy8I GTNNAC 3 cut(s) 170, 560, 1591
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 5 cut(s) 128, 643, 849, 1306, 1422
HpyCH4III ACNGT 4 cut(s) 403, 916, 1351, 1762
HpyCH4IV ACGT 4 cut(s) 267, 874, 1481, 1614
HpyCH4V TGCA 7 cut(s) 197, 440, 686, 794, 800, 1403, 1704
HpyF10VI GCNNNNNNNGC 3 cut(s) 806, 1109, 1252
HpyF3I CTNAG 5 cut(s) 1004, 1113, 1263, 1278, 1527
HpySE526I ACGT 4 cut(s) 267, 874, 1481, 1614
HspAI GCGC 2 cut(s) 1466, 1583
Kzo9I GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 5 cut(s) 1239, 1303, 1387, 1606, 1725
LweI GCATC 3 cut(s) 803, 1416, 1495
MaeI CTAG 5 cut(s) 224, 324, 1019, 1334, 1709
MaeII ACGT 4 cut(s) 267, 874, 1481, 1614
MaeIII GTNAC 3 cut(s) 160, 382, 1477
MalI GATC 9 cut(s) 261, 280, 445, 710, 773, 1016, 1089, 1242, 1259
MboI GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
MboII GAAGA 7 cut(s) 114, 280, 716, 1046, 1218, 1250, 1507
MflI RGATCY 1 cut(s) 278
MlyI GAGTC 2 cut(s) 71, 913
MmeI TCCRAC 3 cut(s) 64, 340, 525
Mph1103I ATGCAT 1 cut(s) 1706
MroXI GAANNNNTTC 3 cut(s) 632, 1213, 1390
MseI TTAA 7 cut(s) 147, 551, 641, 776, 1149, 1517, 1777
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 4 cut(s) 730, 758, 1541, 1655
MspI CCGG 2 cut(s) 64, 81
Mva1269I GAATGC 2 cut(s) 800, 1704
MvnI CGCG 2 cut(s) 676, 1421
MwoI GCNNNNNNNGC 3 cut(s) 806, 1109, 1252
NcoI CCATGG 1 cut(s) 839
NdeII GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
NmeAIII GCCGAG 1 cut(s) 927
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 1706
NspI RCATGY 2 cut(s) 436, 1704
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 4 cut(s) 453, 693, 1183, 1548
PaqCI CACCTGC 1 cut(s) 345
PctI GAATGC 2 cut(s) 800, 1704
PdmI GAANNNNTTC 3 cut(s) 632, 1213, 1390
PflMI CCANNNNNTGG 2 cut(s) 179, 359
PkrI GCNGC 8 cut(s) 62, 1254, 1318, 1402, 1423, 1543, 1621, 1740
PleI GAGTC 2 cut(s) 71, 912
PpsI GAGTC 2 cut(s) 71, 912
Ppu21I YACGTR 1 cut(s) 875
PshAI GACNNNNGTC 2 cut(s) 81, 311
PshBI ATTAAT 1 cut(s) 1149
PsiI TTATAA 1 cut(s) 944
PspPI GGNCC 2 cut(s) 34, 46
PstNI CAGNNNCTG 2 cut(s) 158, 401
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 730
RsaI GTAC 3 cut(s) 266, 881, 1724
RsaNI GTAC 3 cut(s) 265, 880, 1723
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 7 cut(s) 147, 551, 641, 776, 1149, 1517, 1777
SatI GCNGC 8 cut(s) 61, 1253, 1317, 1401, 1422, 1542, 1620, 1739
Sau3AI GATC 9 cut(s) 259, 278, 443, 708, 771, 1014, 1087, 1240, 1257
Sau96I GGNCC 2 cut(s) 34, 46
SchI GAGTC 2 cut(s) 71, 913
SfaNI GCATC 3 cut(s) 803, 1416, 1495
SfcI CTRYAG 2 cut(s) 393, 1456
SinI GGWCC 2 cut(s) 34, 46
SmiMI CAYNNNNRTG 1 cut(s) 431
SnaBI TACGTA 1 cut(s) 875
SphI GCATGC 1 cut(s) 436
SsiI CCGC 6 cut(s) 60, 758, 921, 1421, 1541, 1655
SspI AATATT 1 cut(s) 866
SspMI CTAG 5 cut(s) 224, 324, 1019, 1334, 1709
StyI CCWWGG 3 cut(s) 839, 1018, 1443
TaaI ACNGT 4 cut(s) 403, 916, 1351, 1762
TaiI ACGT 4 cut(s) 270, 877, 1484, 1617
TaqI TCGA 4 cut(s) 132, 669, 763, 1649
TatI WGTACW 1 cut(s) 879
TauI GCSGC 3 cut(s) 63, 1424, 1544
Tru1I TTAA 7 cut(s) 147, 551, 641, 776, 1149, 1517, 1777
Tru9I TTAA 7 cut(s) 147, 551, 641, 776, 1149, 1517, 1777
TscAI CASTG 3 cut(s) 1402, 1740, 1765
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 5 cut(s) 1252, 1316, 1400, 1619, 1738
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 2 cut(s) 1133, 1514
TspRI CASTG 3 cut(s) 1402, 1740, 1765
Van91I CCANNNNNTGG 2 cut(s) 179, 359
VpaK11BI GGWCC 2 cut(s) 34, 46
VspI ATTAAT 1 cut(s) 1149
XapI RAATTY 2 cut(s) 1388, 1600
XceI RCATGY 2 cut(s) 436, 1704
XmaJI CCTAGG 1 cut(s) 1018
XmnI GAANNNNTTC 3 cut(s) 632, 1213, 1390
XspI CTAG 5 cut(s) 224, 324, 1019, 1334, 1709
Zsp2I ATGCAT 1 cut(s) 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.