Rh5CG086700

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008848
Physical Location & Seq
Forward (+)
6820272 .. 6825823
5552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG086700.1

Sequence Viewer

Length: 1614 bp
ATGGCAAGAGCAGCTTTGTTCATCATTTTTCTGGTTCTCCAATTACTCCATTTGGGAAGCAGCCGGTCTGTTAAAGACTCTAATCTGCTCATATCCGATGGCTTAGACGACAAGTTGATCAGCCAGTCTTCTTCACTCCTACAAGTAGTGGATGATCTTAAAGCAGATACAGTGACTTGCGAACCCATTTATGGCTTCTTGCCTTGTGCAACTTCAGTCTGGGGGCTGGCTTTCATGGTAATCGTCTATGAGATTTTGCTCTACCTTGCAAATAGCTGTATCACAAGTGGATCTGAACTCTTCTTCAAAATGTATGGGACCGGATTCTTTGGTGCTAGTGTCTTCCACTTGCTCGGCACAATACCACAAGTGGGAATTGTTGTTGTGACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAACATTGGCATGGTTGCAGGGTCAGCAATCATGCTTCTTACATTAGTCTGGGGTTCTGTTGTTGCATTTGGCAGCTATGACATCTCCAAGTCTCCAACTTCTTCAAATCGAGGACATAAGAAGCGCTTCAGTATCACTGGTTATGTAACAACTGATGCTGAAACCAGCTGTACCGCAAGAATCATGATGGTGTCATTGATCCCCTTTCTTATTCTTCAAGTGGCTAAAGTCCTCAATTCAACCTCAGGGATTCGTATTGTGATCTTAATTTCTCTACTAGTTTCATTTGCATTCCTTGTCGCTTACTGCATCTATCAGGTCTTTGAGCCATGGATTCAGAACAGGAGACTTGAATTTTTGATGAGTAAATATGTACAGAGAACCTTGTCACCGAGTTTTCTCACTGCAAGCGGCGAGCCTAATGTATCAAATATACTAGAGGTGTTTTCTAGAATCGACCAGAATCATAATCAGTCTATATCAGCAAAAGAACTGAGAACATTGATCCTAGGAATACAAATAGGGGAAGTAGGTCTGGAGAGTGATTATGTAGAAAAGGTGATGGAGGACTTCGATATATCTGGTGATTCTCAAATATCTGAGCAAGAGTTTGTTAATGGAATCTCAAAATGGGTTAATGCAAGGCCCTCTGTCAATCGTCAAGGTCAAGGAGATAGAAGGTTTTTAAGCAGCAAGTCCGAGGAAGCTTTGGAAGGGCAGCAGAAGCAGGTGGCTAGAAAAAAGAAGAGTAAGCTTACCGATAAATCTTGGACAAATCAAATCAAGGCTGCTTGTCTCTTGATCCTCGGTATTGGCATTATGATTTTGCTTGCAAATCCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTCACCTCTTATGTTTTGATACCCTTGGCTATCAACTATGGACTGGCATTGCAAATAATAACTTCTGCTCAAGATAAGACTGAGAATGCCATCTCTTTAGCACTTTCAGAGATTTATAACAGCGTGTTCATGAACAACGTTATGGGATTGACCATCTTCTTGGCACTTGTTTACATACGTAATTTATCTTGGGATGTCTCTGCATCAGTTTTAGTTGTTCTGATCATTTGCTCAAGTGTGTCTTCTCTCTGCTGGGCTTTTTCTTGCTGCTTCTCTGGGTTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

537

Amino Acids

58.78

Weight (kDa)

5.74

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 289 - 353 4.4e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
AarI CACCTGC 1 cut(s) 1153
Acc36I ACCTGC 1 cut(s) 1153
AciI CCGC 2 cut(s) 609, 846
AclI AACGTT 1 cut(s) 1464
AclWI GGATC 4 cut(s) 298, 628, 934, 1231
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 2 cut(s) 198, 547
AfaI GTAC 2 cut(s) 607, 810
AfeI AGCGCT 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 191, 371
AgsI TTSAA 5 cut(s) 307, 540, 653, 675, 788
AhlI ACTAGT 1 cut(s) 712
AluBI AGCT 6 cut(s) 14, 276, 510, 603, 1142, 1189
AluI AGCT 6 cut(s) 14, 276, 510, 603, 1142, 1189
Alw26I GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
AlwI GGATC 4 cut(s) 298, 628, 934, 1231
AlwNI CAGNNNCTG 2 cut(s) 404, 413
Aor51HI AGCGCT 1 cut(s) 560
AoxI GGCC 1 cut(s) 1079
ApeKI GCWGC 8 cut(s) 11, 60, 507, 1125, 1153, 1223, 1307, 1593
ApoI RAATTY 1 cut(s) 788
Asp700I GAANNNNTTC 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 943
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 318, 1080
AsuHPI GGTGA 4 cut(s) 816, 1006, 1031, 1321
AvaII GGWCC 1 cut(s) 318
AvrII CCTAGG 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 679
BbsI GAAGAC 3 cut(s) 120, 334, 1560
BbvI GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BccI CCATC 5 cut(s) 92, 616, 991, 1424, 1487
BclI TGATCA 2 cut(s) 117, 1548
BcoDI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BcuI ACTAGT 1 cut(s) 712
BfaI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
BfmI CTRYAG 2 cut(s) 405, 414
BfoI RGCGCY 1 cut(s) 562
BfuAI ACCTGC 1 cut(s) 1153
BisI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
BlnI CCTAGG 1 cut(s) 943
BlsI GCNGC 9 cut(s) 13, 62, 509, 848, 1127, 1155, 1225, 1309, 1595
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 318, 1080
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 4 cut(s) 580, 753, 1323, 1538
BpiI GAAGAC 3 cut(s) 120, 334, 1560
BpmI CTGGAG 2 cut(s) 411, 992
BpuEI CTTGAG 2 cut(s) 1380, 1543
BsaAI YACGTR 1 cut(s) 1505
BsaJI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 371
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 4 cut(s) 124, 394, 577, 1374
Bse21I CCTNAGG 1 cut(s) 679
Bse3DI GCAATG 2 cut(s) 429, 1373
BseDI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BseGI GGATG 3 cut(s) 157, 1314, 1525
BseLI CCNNNNNNNGG 2 cut(s) 191, 371
BseMI GCAATG 2 cut(s) 429, 1373
BseMII CTCAG 4 cut(s) 693, 920, 1026, 1398
BseNI ACTGG 4 cut(s) 124, 394, 577, 1374
BseXI GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BseYI CCCAGC 1 cut(s) 1578
BshFI GGCC 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 64, 321
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 191, 371
BsmAI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 2 cut(s) 725, 1417
BsnI GGCC 1 cut(s) 1081
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Bsp19I CCATGG 1 cut(s) 764
BspACI CCGC 2 cut(s) 609, 846
BspANI GGCC 1 cut(s) 1081
BspCNI CTCAG 4 cut(s) 692, 921, 1027, 1399
BspHI TCATGA 2 cut(s) 618, 1455
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 2 cut(s) 409, 418
BspMI ACCTGC 1 cut(s) 1153
BspPI GGATC 4 cut(s) 298, 628, 934, 1231
BsrDI GCAATG 2 cut(s) 429, 1373
BsrFI RCCGGY 1 cut(s) 63
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 4 cut(s) 124, 394, 577, 1374
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BssMI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
BssT1I CCWWGG 3 cut(s) 764, 943, 1350
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 305, 1175
BstAPI GCANNNNNTGC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 1505
BstC8I GCNNGC 5 cut(s) 228, 844, 851, 1266, 1312
BstDEI CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
BstDSI CCRYGG 1 cut(s) 764
BstF5I GGATG 3 cut(s) 157, 1314, 1525
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 8 cut(s) 120, 157, 293, 636, 699, 942, 1239, 1551
BstMAI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BstMBI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
BstMWI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
BstSFI CTRYAG 2 cut(s) 405, 414
BstSNI TACGTA 1 cut(s) 1505
BstV1I GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BstV2I GAAGAC 3 cut(s) 120, 334, 1560
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 1486
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 679
BsuRI GGCC 1 cut(s) 1081
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 3 cut(s) 157, 1314, 1525
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 3 cut(s) 177, 570, 837
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 5 cut(s) 228, 844, 851, 1266, 1312
CaiI CAGNNNCTG 2 cut(s) 404, 413
CciI TCATGA 2 cut(s) 618, 1455
CfoI GCGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 318, 1080
Csp6I GTAC 2 cut(s) 606, 809
CviAII CATG 7 cut(s) 235, 445, 466, 619, 765, 1279, 1456
CviQI GTAC 2 cut(s) 606, 809
DdeI CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
DpnI GATC 8 cut(s) 119, 156, 292, 635, 698, 941, 1238, 1550
DpnII GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Eam1104I CTCTTC 2 cut(s) 305, 1175
EarI CTCTTC 2 cut(s) 305, 1175
Eco105I TACGTA 1 cut(s) 1505
Eco130I CCWWGG 3 cut(s) 764, 943, 1350
Eco47I GGWCC 1 cut(s) 318
Eco47III AGCGCT 1 cut(s) 560
Eco57I CTGAAG 2 cut(s) 198, 547
Eco81I CCTNAGG 1 cut(s) 679
EcoO109I RGGNCCY 1 cut(s) 1080
EcoT14I CCWWGG 3 cut(s) 764, 943, 1350
ErhI CCWWGG 3 cut(s) 764, 943, 1350
FaeI CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
FalI AAGNNNNNCTT 7 cut(s) 30, 545, 577, 1303, 1335, 1552, 1584
FaqI GGGAC 1 cut(s) 331
FatI CATG 7 cut(s) 234, 444, 465, 618, 764, 1278, 1455
FbaI TGATCA 2 cut(s) 117, 1548
Fnu4HI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
FokI GGATG 3 cut(s) 164, 1301, 1532
Fsp4HI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
FspBI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
GlaI GCGC 1 cut(s) 560
GluI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
GsaI CCCAGC 1 cut(s) 1582
GsuI CTGGAG 2 cut(s) 411, 992
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 1 cut(s) 1081
HapII CCGG 2 cut(s) 64, 321
HhaI GCGC 1 cut(s) 561
Hin1II CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HindIII AAGCTT 2 cut(s) 1140, 1187
HinfI GANTC 9 cut(s) 77, 324, 615, 685, 769, 888, 898, 1022, 1056
HpaII CCGG 2 cut(s) 64, 321
HphI GGTGA 4 cut(s) 816, 1006, 1031, 1321
Hpy166II GTNNAC 1 cut(s) 1498
Hpy188I TCNGA 7 cut(s) 97, 295, 774, 1036, 1135, 1435, 1548
Hpy188III TCNNGA 6 cut(s) 619, 885, 971, 1234, 1397, 1456
Hpy8I GTNNAC 1 cut(s) 1498
HpyAV CCTTC 3 cut(s) 1107, 1142, 1329
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 1464, 1504
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
HpyF3I CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
HpySE526I ACGT 2 cut(s) 1464, 1504
Hsp92II CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
HspAI GCGC 1 cut(s) 559
Ksp22I TGATCA 2 cut(s) 117, 1548
Kzo9I GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Lsp1109I GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
LweI GCATC 4 cut(s) 580, 753, 1323, 1538
MaeI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
MaeII ACGT 2 cut(s) 1464, 1504
MaeIII GTNAC 5 cut(s) 172, 385, 394, 580, 822
MalI GATC 8 cut(s) 119, 156, 292, 635, 698, 941, 1238, 1550
MboI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
MflI RGATCY 1 cut(s) 290
MluCI AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
MslI CAYNNNNRTG 2 cut(s) 443, 623
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 2 cut(s) 64, 321
Mva1269I GAATGC 2 cut(s) 725, 1417
MwoI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
NcoI CCATGG 1 cut(s) 764
NdeII GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
NlaIII CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 3 cut(s) 172, 385, 822
PagI TCATGA 2 cut(s) 618, 1455
PaqCI CACCTGC 1 cut(s) 1153
PctI GAATGC 2 cut(s) 725, 1417
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 8 cut(s) 324, 615, 685, 769, 888, 898, 1022, 1056
PkrI GCNGC 9 cut(s) 13, 62, 509, 848, 1127, 1155, 1225, 1309, 1595
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 1505
PsiI TTATAA 1 cut(s) 1443
Psp1406I AACGTT 1 cut(s) 1464
PspFI CCCAGC 1 cut(s) 1578
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 318, 1080
PstI CTGCAG 2 cut(s) 409, 418
PstNI CAGNNNCTG 2 cut(s) 404, 413
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 603
RsaI GTAC 2 cut(s) 607, 810
RsaNI GTAC 2 cut(s) 606, 809
RseI CAYNNNNRTG 2 cut(s) 443, 623
SaqAI TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
SatI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
Sau3AI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Sau96I GGNCC 2 cut(s) 318, 1080
SchI GAGTC 1 cut(s) 71
SfaNI GCATC 4 cut(s) 580, 753, 1323, 1538
SfcI CTRYAG 2 cut(s) 405, 414
SinI GGWCC 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 443, 623
SmlI CTYRAG 2 cut(s) 1395, 1558
SmoI CTYRAG 2 cut(s) 1395, 1558
SnaBI TACGTA 1 cut(s) 1505
SpeI ACTAGT 1 cut(s) 712
Sse9I AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
SsiI CCGC 2 cut(s) 609, 846
SspMI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
StyI CCWWGG 3 cut(s) 764, 943, 1350
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 1467, 1507
TaqI TCGA 3 cut(s) 544, 891, 1008
TasI AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 8 cut(s) 324, 615, 685, 769, 888, 898, 1022, 1056
Tru1I TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
Tru9I TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
TscAI CASTG 3 cut(s) 177, 577, 844
TseFI GTSAC 3 cut(s) 172, 385, 822
TseI GCWGC 8 cut(s) 11, 60, 507, 1125, 1153, 1223, 1307, 1593
Tsp45I GTSAC 3 cut(s) 172, 385, 822
TspDTI ATGAA 7 cut(s) 10, 223, 449, 708, 1311, 1444, 1472
TspRI CASTG 3 cut(s) 177, 577, 844
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 788
XbaI TCTAGA 1 cut(s) 884
XmaJI CCTAGG 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.