FvH4_3g08181

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008848
Physical Location & Seq
Reverse (-)
4808914 .. 4814106
5193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08181.t1

Sequence Viewer

Length: 1689 bp
ATGGCAAGAGCAGCTTTCTTCATCATTTTTCTGGTTCTCCAATTACTCCATTTGGGACGCTGCCGGTCTGTTAGAGACTCTAATGTGCTCATTTCCGATGGCTTAGATGACAAGTTGATCAGCCAGTATTCTTCACTCCTACAAGTAGTGGATGATCTTAAGGCAGATACAGTGACTTGCGAACCCATTTATGGATTCTTGCCTTGTGCAACTTCATTCTGGGGGCTGGCTTTCATGGTAATTGTCTACGAGATCTTGCTCTACCTTGCAAATAGCTTCATTACCAGTGGATCCGAACTCTTCTTTCAAATGTATGGGACAGGATTATTTGGTGCTAGTGTCTTCCACTTGCTTGGCACAGTACCACAAGTCGGAATTGTTCTTGTGACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAACATTGGCATGGTTGCAGGGTCAGCAATCATGCTTCTTACACTAGTTTGGGGGTCTGTTGTTGCATTTGGCAGCTATGACATCTCTGACTCTCCAACTTCTTCAAATCGAGGACATAAGAACCGCTTTAGTATCACCGGTTATGGTGTAACAACTGATGCTGAAACCCGCTCTACTGCAAGAATTATGATGGTGTCATTGATCCCCTTTCTCATTCTTCAAGTGGCTAAAGTCCTTTCCTCAACCTCAGGGATTCGTATCGTGATCTTAGTTTCTCTACTTGTTTCATTTGCATTTCTTGTCACTTACTGCATCTACCAGGTCTTTCAACCATGGATTCAGAACAGAAGGCTTGAATTTCTGATGAGTAAATATGTACAGAAAACCTTGTTACCAAGTTTTCTCACTGCAAGCGGAGACCCTAATGTATCAAATATACAGGAGGTGTTTACTAGAATCGACCAGAATCATAATACATATATATCAGCATATGAACTGAGGGCATTGGTCCTAGGAATACAAATAGGAGAAGTAGGTTTGGATAGTGATTATGTAGATAAGGCAATGGAGGACTTTGACATATCTGGTGATTCTCAAATATCTGAGCAAGAGTTTGTTGATGGAATCTCAAAATGGGTTAATGCAACGCCCTCTGCCAATGGTCAAGGTCAAGGACATAGAAGGTTTTTAAGCAGCAAAACTGAGGAAGCTTTGGGAGGGAAGCAGAAGCAGGTCTCTAAGAAAAAGAAGAGTAAGCTTACTGATAAAACTTGGACGAATCAACTTAAGGCTGCTTGTCTATTGATCCTCGGCATTGGCATTATGATCTTGCTTGCAACACCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTTACCTCTTATGTTTTGATACCCTTGGCTATCAACTATGGACAGGCATTGCAACTAATAACTTCTGCTCAAGATAAGACAGAGAATGCCGTATCTTTATCACTTTCAGAGATTTATAATGGCGTATTCATGAACAACGTTATGGGATTGACCATCTTCTTGGCACTTGTTTACATACGTAATTTATCATGGGATGTCTGTGCAGCAGTCTTGGCTGTTCTCATCATTTGTTCAGTAATGGGTCTTTATACCAGCTTCAGCACAAAGTTTCCGTTTTGGACATGTATTTTAGCATACATCATGTACCCCATATCATTGCTGATGATTTATGTCCTTACCAGTTATCTTGGATGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

563

Amino Acids

61.8

Weight (kDa)

5.65

Isoelectric Point (pI)

27.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 290 - 354 1.8e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1446
Acc36I ACCTGC 1 cut(s) 1156
AccBSI CCGCTC 1 cut(s) 606
AccI GTMKAC 1 cut(s) 246
AciI CCGC 3 cut(s) 559, 604, 849
AclI AACGTT 1 cut(s) 1467
AclWI GGATC 4 cut(s) 285, 298, 631, 1234
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 1570
AfaI GTAC 3 cut(s) 363, 813, 1634
AfiI CCNNNNNNNGG 2 cut(s) 191, 371
AflII CTTAAG 2 cut(s) 158, 1220
AflIII ACRYGT 1 cut(s) 1610
AgeI ACCGGT 1 cut(s) 572
AgsI TTSAA 5 cut(s) 308, 540, 656, 764, 791
AhlI ACTAGT 1 cut(s) 478
AjnI CCWGG 1 cut(s) 753
AluBI AGCT 6 cut(s) 14, 276, 510, 1145, 1192, 1584
AluI AGCT 6 cut(s) 14, 276, 510, 1145, 1192, 1584
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 4 cut(s) 69, 846, 1174, 1280
AlwI GGATC 4 cut(s) 285, 298, 631, 1234
AlwNI CAGNNNCTG 2 cut(s) 404, 413
ApeKI GCWGC 7 cut(s) 11, 60, 507, 1128, 1226, 1310, 1532
ApoI RAATTY 1 cut(s) 791
AsiGI ACCGGT 1 cut(s) 572
AspA2I CCTAGG 1 cut(s) 946
AspS9I GGNCC 1 cut(s) 943
AsuHPI GGTGA 2 cut(s) 562, 1034
AvaII GGWCC 1 cut(s) 943
AvrII CCTAGG 1 cut(s) 946
AxyI CCTNAGG 1 cut(s) 682
BamHI GGATCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 7 cut(s) 23, 47, 519, 1140, 1213, 1297, 1544
BccI CCATC 5 cut(s) 92, 619, 1049, 1490, 1674
BceAI ACGGC 1 cut(s) 1403
BciT130I CCWGG 1 cut(s) 755
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 4 cut(s) 69, 846, 1174, 1280
BcuI ACTAGT 1 cut(s) 478
BfaI CTAG 4 cut(s) 336, 479, 888, 947
BfmI CTRYAG 2 cut(s) 405, 414
BfrI CTTAAG 2 cut(s) 158, 1220
BfuAI ACCTGC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 252
BisI GCNGC 7 cut(s) 12, 61, 508, 1129, 1227, 1311, 1533
BlnI CCTAGG 1 cut(s) 946
BlsI GCNGC 7 cut(s) 13, 62, 509, 1130, 1228, 1312, 1534
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 3 cut(s) 583, 756, 1326
BpiI GAAGAC 1 cut(s) 334
BpmI CTGGAG 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 1383
BsaAI YACGTR 1 cut(s) 1508
BsaBI GATNNNNATC 1 cut(s) 692
BsaI GGTCTC 2 cut(s) 846, 1174
BsaJI CCNNGG 4 cut(s) 767, 946, 1243, 1353
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 371
Bse118I RCCGGY 2 cut(s) 63, 572
Bse1I ACTGG 4 cut(s) 124, 285, 394, 1668
Bse21I CCTNAGG 1 cut(s) 682
Bse3DI GCAATG 4 cut(s) 429, 1005, 1376, 1643
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 4 cut(s) 767, 946, 1243, 1353
BseGI GGATG 4 cut(s) 157, 1317, 1528, 1685
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 191, 371
BseMI GCAATG 4 cut(s) 429, 1005, 1376, 1643
BseMII CTCAG 4 cut(s) 696, 923, 1029, 1128
BseNI ACTGG 4 cut(s) 124, 285, 394, 1668
BseXI GCAGC 7 cut(s) 23, 47, 519, 1140, 1213, 1297, 1544
BsgI GTGCAG 1 cut(s) 1551
BshTI ACCGGT 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 2 cut(s) 64, 573
BslFI GGGAC 2 cut(s) 69, 331
BslI CCNNNNNNNGG 2 cut(s) 191, 371
BsmAI GTCTC 4 cut(s) 69, 846, 1174, 1280
BsmFI GGGAC 2 cut(s) 69, 331
BsmI GAATGC 1 cut(s) 1420
Bso31I GGTCTC 2 cut(s) 846, 1174
Bsp1286I GDGCHC 1 cut(s) 90
Bsp1407I TGTACA 1 cut(s) 811
Bsp143I GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 3 cut(s) 559, 604, 849
BspCNI CTCAG 4 cut(s) 695, 924, 1030, 1129
BspHI TCATGA 1 cut(s) 1458
BspLI GGNNCC 1 cut(s) 292
BspMAI CTGCAG 2 cut(s) 409, 418
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 4 cut(s) 285, 298, 631, 1234
BspTI CTTAAG 2 cut(s) 158, 1220
BspTNI GGTCTC 2 cut(s) 846, 1174
BsrBI CCGCTC 1 cut(s) 606
BsrDI GCAATG 4 cut(s) 429, 1005, 1376, 1643
BsrFI RCCGGY 2 cut(s) 63, 572
BsrGI TGTACA 1 cut(s) 811
BsrI ACTGG 4 cut(s) 124, 285, 394, 1668
BssAI RCCGGY 2 cut(s) 63, 572
BssECI CCNNGG 4 cut(s) 767, 946, 1243, 1353
BssMI GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
BssT1I CCWWGG 3 cut(s) 767, 946, 1353
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 2 cut(s) 172, 361
Bst6I CTCTTC 2 cut(s) 305, 1178
BstAFI CTTAAG 2 cut(s) 158, 1220
BstAPI GCANNNNNTGC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 811
BstBAI YACGTR 1 cut(s) 1508
BstC8I GCNNGC 4 cut(s) 228, 847, 1269, 1315
BstDEI CTNAG 7 cut(s) 103, 682, 703, 932, 1038, 1137, 1173
BstDSI CCRYGG 1 cut(s) 767
BstF5I GGATG 4 cut(s) 157, 1317, 1528, 1685
BstKTI GATC 8 cut(s) 120, 157, 255, 293, 639, 702, 1242, 1263
BstMAI GTCTC 4 cut(s) 69, 846, 1174, 1280
BstMBI GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
BstMWI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1541
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 1 cut(s) 1614
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 2 cut(s) 405, 414
BstSNI TACGTA 1 cut(s) 1508
BstV1I GCAGC 7 cut(s) 23, 47, 519, 1140, 1213, 1297, 1544
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 252, 290
BstXI CCANNNNNNTGG 2 cut(s) 353, 1489
BstYI RGATCY 2 cut(s) 252, 290
Bsu36I CCTNAGG 1 cut(s) 682
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 4 cut(s) 157, 1317, 1528, 1685
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 3 cut(s) 177, 292, 840
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 4 cut(s) 228, 847, 1269, 1315
CaiI CAGNNNCTG 2 cut(s) 404, 413
CciI TCATGA 1 cut(s) 1458
Cfr10I RCCGGY 2 cut(s) 63, 572
Cfr13I GGNCC 1 cut(s) 943
CseI GACGC 1 cut(s) 66
CsiI ACCWGGT 1 cut(s) 753
Csp6I GTAC 3 cut(s) 362, 812, 1633
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 9 cut(s) 235, 445, 466, 768, 1282, 1459, 1518, 1611, 1630
CviQI GTAC 3 cut(s) 362, 812, 1633
DdeI CTNAG 7 cut(s) 103, 682, 703, 932, 1038, 1137, 1173
DpnI GATC 8 cut(s) 119, 156, 254, 292, 638, 701, 1241, 1262
DpnII GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
Eam1104I CTCTTC 2 cut(s) 305, 1178
EarI CTCTTC 2 cut(s) 305, 1178
Eco105I TACGTA 1 cut(s) 1508
Eco130I CCWWGG 3 cut(s) 767, 946, 1353
Eco31I GGTCTC 2 cut(s) 846, 1174
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 1 cut(s) 1570
Eco81I CCTNAGG 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 3 cut(s) 767, 946, 1353
ErhI CCWWGG 3 cut(s) 767, 946, 1353
FaeI CATG 9 cut(s) 238, 448, 469, 771, 1285, 1462, 1521, 1614, 1633
FalI AAGNNNNNCTT 5 cut(s) 30, 545, 577, 1306, 1338
FaqI GGGAC 2 cut(s) 69, 331
FatI CATG 9 cut(s) 234, 444, 465, 767, 1281, 1458, 1517, 1610, 1629
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 7 cut(s) 12, 61, 508, 1129, 1227, 1311, 1533
FokI GGATG 3 cut(s) 164, 1304, 1535
Fsp4HI GCNGC 7 cut(s) 12, 61, 508, 1129, 1227, 1311, 1533
FspBI CTAG 4 cut(s) 336, 479, 888, 947
GluI GCNGC 7 cut(s) 12, 61, 508, 1129, 1227, 1311, 1533
GsuI CTGGAG 1 cut(s) 411
HapII CCGG 2 cut(s) 64, 573
HgaI GACGC 1 cut(s) 66
Hin1II CATG 9 cut(s) 238, 448, 469, 771, 1285, 1462, 1521, 1614, 1633
HindIII AAGCTT 2 cut(s) 1143, 1190
HpaII CCGG 2 cut(s) 64, 573
HphI GGTGA 2 cut(s) 562, 1034
Hpy166II GTNNAC 3 cut(s) 247, 885, 1501
Hpy188I TCNGA 8 cut(s) 97, 295, 374, 523, 777, 798, 1039, 1438
Hpy188III TCNNGA 3 cut(s) 697, 1400, 1459
Hpy8I GTNNAC 3 cut(s) 247, 885, 1501
HpyAV CCTTC 3 cut(s) 777, 1110, 1332
HpyCH4III ACNGT 2 cut(s) 172, 361
HpyCH4IV ACGT 2 cut(s) 1467, 1507
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1541
HpyF3I CTNAG 7 cut(s) 103, 682, 703, 932, 1038, 1137, 1173
HpySE526I ACGT 2 cut(s) 1467, 1507
Hsp92II CATG 9 cut(s) 238, 448, 469, 771, 1285, 1462, 1521, 1614, 1633
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
Lsp1109I GCAGC 7 cut(s) 23, 47, 519, 1140, 1213, 1297, 1544
LweI GCATC 3 cut(s) 583, 756, 1326
MabI ACCWGGT 1 cut(s) 753
MaeI CTAG 4 cut(s) 336, 479, 888, 947
MaeII ACGT 2 cut(s) 1467, 1507
MaeIII GTNAC 6 cut(s) 172, 385, 394, 583, 736, 825
MalI GATC 8 cut(s) 119, 156, 254, 292, 638, 701, 1241, 1262
MbiI CCGCTC 1 cut(s) 606
MboI GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
MboII GAAGA 8 cut(s) 10, 123, 292, 334, 528, 644, 1195, 1477
MflI RGATCY 2 cut(s) 252, 290
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 6 cut(s) 41, 240, 375, 618, 791, 1510
MlyI GAGTC 2 cut(s) 71, 518
MmeI TCCRAC 2 cut(s) 352, 554
MseI TTAA 5 cut(s) 159, 1074, 1124, 1221, 1687
MslI CAYNNNNRTG 1 cut(s) 443
MspCI CTTAAG 2 cut(s) 158, 1220
MspI CCGG 2 cut(s) 64, 573
MspR9I CCNGG 1 cut(s) 755
Mva1269I GAATGC 1 cut(s) 1420
MvaI CCWGG 1 cut(s) 755
MwoI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1541
NcoI CCATGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 925
NdeII GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
NlaIII CATG 9 cut(s) 238, 448, 469, 771, 1285, 1462, 1521, 1614, 1633
NlaIV GGNNCC 1 cut(s) 292
NmeAIII GCCGAG 1 cut(s) 1224
NmuCI GTSAC 3 cut(s) 172, 385, 736
NspI RCATGY 1 cut(s) 1614
PagI TCATGA 1 cut(s) 1458
PciI ACATGT 1 cut(s) 1610
PctI GAATGC 1 cut(s) 1420
PfeI GAWTC 8 cut(s) 195, 688, 772, 891, 901, 1025, 1059, 1213
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 7 cut(s) 13, 62, 509, 1130, 1228, 1312, 1534
PleI GAGTC 2 cut(s) 71, 518
PpsI GAGTC 2 cut(s) 71, 518
Ppu21I YACGTR 1 cut(s) 1508
PscI ACATGT 1 cut(s) 1610
PsiI TTATAA 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 1467
Psp6I CCWGG 1 cut(s) 753
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 943
PstI CTGCAG 2 cut(s) 409, 418
PstNI CAGNNNCTG 2 cut(s) 404, 413
PsuI RGATCY 2 cut(s) 252, 290
RsaI GTAC 3 cut(s) 363, 813, 1634
RsaNI GTAC 3 cut(s) 362, 812, 1633
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 5 cut(s) 159, 1074, 1124, 1221, 1687
SatI GCNGC 7 cut(s) 12, 61, 508, 1129, 1227, 1311, 1533
Sau3AI GATC 8 cut(s) 117, 154, 252, 290, 636, 699, 1239, 1260
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 2 cut(s) 71, 518
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 1 cut(s) 90
SexAI ACCWGGT 1 cut(s) 753
SfaNI GCATC 3 cut(s) 583, 756, 1326
SfcI CTRYAG 2 cut(s) 405, 414
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 1 cut(s) 443
SmlI CTYRAG 3 cut(s) 158, 1220, 1398
SmoI CTYRAG 3 cut(s) 158, 1220, 1398
SnaBI TACGTA 1 cut(s) 1508
SpeI ACTAGT 1 cut(s) 478
Sse9I AATT 6 cut(s) 41, 240, 375, 618, 791, 1510
SsiI CCGC 3 cut(s) 559, 604, 849
SspMI CTAG 4 cut(s) 336, 479, 888, 947
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 3 cut(s) 767, 946, 1353
TaaI ACNGT 2 cut(s) 172, 361
TaiI ACGT 2 cut(s) 1470, 1510
TaqI TCGA 2 cut(s) 544, 894
TasI AATT 6 cut(s) 41, 240, 375, 618, 791, 1510
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 8 cut(s) 195, 688, 772, 891, 901, 1025, 1059, 1213
Tru1I TTAA 5 cut(s) 159, 1074, 1124, 1221, 1687
Tru9I TTAA 5 cut(s) 159, 1074, 1124, 1221, 1687
TscAI CASTG 3 cut(s) 177, 292, 847
TseFI GTSAC 3 cut(s) 172, 385, 736
TseI GCWGC 7 cut(s) 11, 60, 507, 1128, 1226, 1310, 1532
Tsp45I GTSAC 3 cut(s) 172, 385, 736
TspGWI ACGGA 1 cut(s) 1590
TspRI CASTG 3 cut(s) 177, 292, 847
Vha464I CTTAAG 2 cut(s) 158, 1220
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 1 cut(s) 791
XceI RCATGY 1 cut(s) 1614
XmaJI CCTAGG 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 4 cut(s) 336, 479, 888, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.