MD10G1284700.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008848
Physical Location & Seq
Reverse (-)
37456466 .. 37460459
3994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1284700.v1.1.491

Sequence Viewer

Length: 1692 bp
ATGTCAAGACCAGCTTTGTTCATCATTTTTCTGGTCCTCCAACTAGTCCATTTAGGAAGCGGCCGGATTGTTAGAGACTCCAGTCTTATATCCGATGGAATAAACGACGAAACAAGCCAATCTTCGATCGTCGAAGCGCTGAATCTTAAAGCAGATTCTGTGACTTGTGAATCAATTTATGGCTTCTTGCCTTGTGCAACTTCAGTTTGGGGTTTTCTTTTTCTGATAATCGTCTACGAGGTCTTGCTCTCCCTCGCAGATCATTACGTTTCAAGAGGATCTGAAATCTTCTTTGAATTGTTTGGGACTGGTATCTTTGGTGCTAGTGCTTTCCACCTGCTTGGCTTGATTCCACAAGTTGGAGTGGTTCTTGTAACTGGAATTACAGCAACTACAGAAACTGCGGAGGAAATGGCAAAAATGAACATGAGCATGCTTGCAGGATCAACAATCATGATTCTTACCCTAATTTGGGGTTCTGTTGTTGCTTTCGGTAGCTCCGACCTCTCGGATTCTCAAACTTCATCAAATTCAGAAAATAAGAACCCCTTCAGTTTAACTGGTTCCGGTGTAAGAACTGATGTCGAAACCCAAGATACAGCAAGAATCATGATGCTCTCGTTGATTCCATATCTCGTTCTTCAACTGGCAAAAATCTTGACTTCAACCACAGCGGTTCGAGTTGTGATCTTAATTTCTCTACTGATTGCATCTGCAATCCTTGCCACTTACATCATCTACCAGTTCTTTCAACCATGGATTCAGAGCAGAAGGCTTGAATATTTGATGCGTAAATACATACCGAAGATACTGCCGAGTCTTCTGACAGTAGACGGAAACGCTAATGAATCCAGTATAAAAGAGCTGTTCCATAGAATAGACAAGAATCATAACACATACATATCAGCAGATGAACTTAGAGCATTGATCCTAGGAATACAAATAGAAGAAATAGGTCTGGATGATGATGATTATGCAGAAGAAGTGATGAAGCAATTTGATCTCTCTGGCGACGCTCAAATAGCTGAGACGGAGTTTGTGAATGGAATCTCAAAATGGATTAATCCAGATAAGACCCCTGCCAACGGTAAAGGTAATGAACATAGATCGCTTTTCAGAAGAAATCATTCCAAGAATGAGACATCTATGGAAGATCAGCAGACACCGCTGATCTCTAAGAAAAAGAAGACTAGGGGTGCTGATACATCGTGGATGAATCTCCTGAAGGCTGCTTTTCTAATTATTCTAGGAACTGCCATAACGGTTTTTCTTTCAATGCCCCTCATGGTAAGTCTCCAAGAGTTTTCCACTGCTGCAAGCATCCCTTCTTTCACGACCTCGTATGTTGTGATACCCTTGGCTACAAATTACAGACTGGCGCTAATGTCTGTATCGTCTGCCAAAGAAAAGACGGAGAATGCCATCTCTTTAACACTGTCTGAGATTTACAACGCGGCATTCATGAACAATATTGTGGGGTTGATCATATTTTTGGCTCTTGTTTATATACGCAATTTATCATGGGATGTCGCTGCTGAAGTTTTAGTTGTTTTAATTATTTCGATAGCGGTTGGTCTTTCTGCAAGCTTCAGCAGAAAGTTCCCATTTTGGACAAGCATTCTAGCATACATTCTGTACCCCATATCACTGCTGCTGGTTTATGTTCTTACCATTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

564

Amino Acids

62.08

Weight (kDa)

5.26

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 283 - 350 4.6e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 345
AccB7I CCANNNNNTGG 1 cut(s) 359
AccI GTMKAC 2 cut(s) 234, 831
AccII CGCG 1 cut(s) 1454
AciI CCGC 6 cut(s) 60, 404, 674, 1166, 1454, 1568
AclWI GGATC 3 cut(s) 286, 451, 922
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 529
AcuI CTGAAG 5 cut(s) 186, 535, 1244, 1557, 1573
AfaI GTAC 1 cut(s) 1637
AfeI AGCGCT 1 cut(s) 138
AfiI CCNNNNNNNGG 4 cut(s) 359, 471, 472, 1085
AgsI TTSAA 7 cut(s) 273, 296, 644, 666, 752, 779, 1275
AhlI ACTAGT 1 cut(s) 43
AluBI AGCT 5 cut(s) 14, 498, 865, 1025, 1587
AluI AGCT 5 cut(s) 14, 498, 865, 1025, 1587
Alw26I GTCTC 4 cut(s) 69, 1022, 1133, 1298
AlwI GGATC 3 cut(s) 286, 451, 922
AlwNI CAGNNNCTG 2 cut(s) 158, 401
Aor51HI AGCGCT 1 cut(s) 138
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 4 cut(s) 1229, 1313, 1532, 1651
ApoI RAATTY 1 cut(s) 529
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 2 cut(s) 548, 1126
AspA2I CCTAGG 1 cut(s) 931
AspLEI GCGC 2 cut(s) 139, 1381
AspS9I GGNCC 1 cut(s) 34
AvaII GGWCC 1 cut(s) 34
AvrII CCTAGG 1 cut(s) 931
BbsI GAAGAC 2 cut(s) 812, 1193
BbvI GCAGC 4 cut(s) 1216, 1300, 1519, 1638
BccI CCATC 3 cut(s) 89, 1430, 1677
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BclI TGATCA 1 cut(s) 1482
BcoDI GTCTC 4 cut(s) 69, 1022, 1133, 1298
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 6 cut(s) 44, 324, 932, 1191, 1247, 1622
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 2 cut(s) 140, 1382
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 6 cut(s) 61, 1230, 1314, 1455, 1533, 1652
BlnI CCTAGG 1 cut(s) 931
BlsI GCNGC 6 cut(s) 62, 1231, 1315, 1456, 1534, 1653
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 565
BmsI GCATC 4 cut(s) 603, 719, 777, 1329
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 812, 1193
BpmI CTGGAG 1 cut(s) 64
BsaJI CCNNGG 3 cut(s) 755, 931, 1356
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 471, 472, 1085
Bse1I ACTGG 8 cut(s) 81, 313, 382, 565, 651, 742, 852, 1380
BseDI CCNNGG 3 cut(s) 755, 931, 1356
BseGI GGATG 5 cut(s) 967, 1218, 1320, 1531, 1688
BseLI CCNNNNNNNGG 4 cut(s) 359, 471, 472, 1085
BseMII CTCAG 2 cut(s) 1017, 1431
BseNI ACTGG 8 cut(s) 81, 313, 382, 565, 651, 742, 852, 1380
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 4 cut(s) 1216, 1300, 1519, 1638
Bsh1236I CGCG 1 cut(s) 1454
Bsh1285I CGRYCG 2 cut(s) 64, 129
BshFI GGCC 1 cut(s) 63
BsiEI CGRYCG 2 cut(s) 64, 129
BsiSI CCGG 2 cut(s) 64, 567
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 4 cut(s) 359, 471, 472, 1085
BsmAI GTCTC 4 cut(s) 69, 1022, 1133, 1298
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 1 cut(s) 319
BsmI GAATGC 3 cut(s) 1423, 1457, 1617
BsnI GGCC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 6 cut(s) 60, 404, 674, 1166, 1454, 1568
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 2 cut(s) 1018, 1432
BspFNI CGCG 1 cut(s) 1454
BspHI TCATGA 3 cut(s) 453, 609, 1461
BspLI GGNNCC 1 cut(s) 565
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 286, 451, 922
BsrI ACTGG 8 cut(s) 81, 313, 382, 565, 651, 742, 852, 1380
BssECI CCNNGG 3 cut(s) 755, 931, 1356
BssT1I CCWWGG 3 cut(s) 755, 931, 1356
Bst4CI ACNGT 4 cut(s) 829, 1088, 1264, 1437
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 4 cut(s) 434, 438, 1318, 1585
BstDEI CTNAG 4 cut(s) 917, 1026, 1176, 1440
BstDSI CCRYGG 1 cut(s) 755
BstF5I GGATG 5 cut(s) 967, 1218, 1320, 1531, 1688
BstFNI CGCG 1 cut(s) 1454
BstH2I RGCGCY 2 cut(s) 140, 1382
BstHHI GCGC 2 cut(s) 139, 1381
BstMAI GTCTC 4 cut(s) 69, 1022, 1133, 1298
BstMCI CGRYCG 2 cut(s) 64, 129
BstMWI GCNNNNNNNGC 3 cut(s) 722, 1022, 1165
BstNSI RCATGY 1 cut(s) 436
BstPAI GACNNNNGTC 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 1 cut(s) 1454
BstV1I GCAGC 4 cut(s) 1216, 1300, 1519, 1638
BstV2I GAAGAC 2 cut(s) 812, 1193
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 755
BtsCI GGATG 5 cut(s) 967, 1218, 1320, 1531, 1688
BtsI GCAGTG 2 cut(s) 1308, 1646
BtsIMutI CAGTG 3 cut(s) 1308, 1433, 1646
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 4 cut(s) 434, 438, 1318, 1585
CaiI CAGNNNCTG 2 cut(s) 158, 401
CciI TCATGA 3 cut(s) 453, 609, 1461
CfoI GCGC 2 cut(s) 139, 1381
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 1 cut(s) 1636
CviAII CATG 8 cut(s) 427, 433, 454, 610, 756, 1285, 1462, 1521
CviQI GTAC 1 cut(s) 1636
DdeI CTNAG 4 cut(s) 917, 1026, 1176, 1440
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 61
Eco130I CCWWGG 3 cut(s) 755, 931, 1356
Eco47I GGWCC 1 cut(s) 34
Eco47III AGCGCT 1 cut(s) 138
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 5 cut(s) 186, 535, 1244, 1557, 1573
EcoT14I CCWWGG 3 cut(s) 755, 931, 1356
ErhI CCWWGG 3 cut(s) 755, 931, 1356
Esp3I CGTCTC 1 cut(s) 1022
FaeI CATG 8 cut(s) 430, 436, 457, 613, 759, 1288, 1465, 1524
FalI AAGNNNNNCTT 7 cut(s) 30, 106, 138, 533, 565, 1309, 1341
FaqI GGGAC 1 cut(s) 319
FatI CATG 8 cut(s) 426, 432, 453, 609, 755, 1284, 1461, 1520
FbaI TGATCA 1 cut(s) 1482
FblI GTMKAC 2 cut(s) 234, 831
Fnu4HI GCNGC 6 cut(s) 61, 1230, 1314, 1455, 1533, 1652
FokI GGATG 4 cut(s) 974, 1225, 1307, 1538
Fsp4HI GCNGC 6 cut(s) 61, 1230, 1314, 1455, 1533, 1652
FspBI CTAG 6 cut(s) 44, 324, 932, 1191, 1247, 1622
GlaI GCGC 2 cut(s) 138, 1380
GluI GCNGC 6 cut(s) 61, 1230, 1314, 1455, 1533, 1652
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 2 cut(s) 140, 1382
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 64, 567
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 2 cut(s) 139, 1381
Hin1II CATG 8 cut(s) 430, 436, 457, 613, 759, 1288, 1465, 1524
Hin6I GCGC 2 cut(s) 137, 1379
HinP1I GCGC 2 cut(s) 137, 1379
HindIII AAGCTT 1 cut(s) 1585
HpaII CCGG 2 cut(s) 64, 567
Hpy166II GTNNAC 2 cut(s) 235, 832
Hpy8I GTNNAC 2 cut(s) 235, 832
Hpy99I CGWCG 3 cut(s) 110, 134, 1016
HpyAV CCTTC 4 cut(s) 559, 765, 1219, 1335
HpyCH4III ACNGT 4 cut(s) 829, 1088, 1264, 1437
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 7 cut(s) 197, 440, 710, 716, 977, 1316, 1583
HpyF10VI GCNNNNNNNGC 3 cut(s) 722, 1022, 1165
HpyF3I CTNAG 4 cut(s) 917, 1026, 1176, 1440
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 8 cut(s) 430, 436, 457, 613, 759, 1288, 1465, 1524
HspAI GCGC 2 cut(s) 137, 1379
Ksp22I TGATCA 1 cut(s) 1482
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 4 cut(s) 1216, 1300, 1519, 1638
LweI GCATC 4 cut(s) 603, 719, 777, 1329
MaeI CTAG 6 cut(s) 44, 324, 932, 1191, 1247, 1622
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 160, 373
MflI RGATCY 1 cut(s) 278
MlyI GAGTC 2 cut(s) 71, 826
MmeI TCCRAC 3 cut(s) 64, 340, 525
MnlI CCTC 8 cut(s) 47, 232, 263, 269, 400, 515, 1292, 1348
MroXI GAANNNNTTC 2 cut(s) 548, 1126
MseI TTAA 7 cut(s) 147, 557, 692, 1062, 1430, 1553, 1690
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 2 cut(s) 674, 1168
MspI CCGG 2 cut(s) 64, 567
Mva1269I GAATGC 3 cut(s) 1423, 1457, 1617
MvnI CGCG 1 cut(s) 1454
MwoI GCNNNNNNNGC 3 cut(s) 722, 1022, 1165
NcoI CCATGG 1 cut(s) 755
NlaIII CATG 8 cut(s) 430, 436, 457, 613, 759, 1288, 1465, 1524
NlaIV GGNNCC 1 cut(s) 565
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 436
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 3 cut(s) 453, 609, 1461
PaqCI CACCTGC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 3 cut(s) 1423, 1457, 1617
PdmI GAANNNNTTC 2 cut(s) 548, 1126
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 6 cut(s) 62, 1231, 1315, 1456, 1534, 1653
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 2 cut(s) 71, 825
PpsI GAGTC 2 cut(s) 71, 825
PshAI GACNNNNGTC 1 cut(s) 81
PshBI ATTAAT 1 cut(s) 1062
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 158, 401
PsuI RGATCY 1 cut(s) 278
PvuI CGATCG 1 cut(s) 129
RsaI GTAC 1 cut(s) 1637
RsaNI GTAC 1 cut(s) 1636
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 7 cut(s) 147, 557, 692, 1062, 1430, 1553, 1690
SatI GCNGC 6 cut(s) 61, 1230, 1314, 1455, 1533, 1652
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 2 cut(s) 71, 826
SfaNI GCATC 4 cut(s) 603, 719, 777, 1329
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 43
SphI GCATGC 1 cut(s) 436
SsiI CCGC 6 cut(s) 60, 404, 674, 1166, 1454, 1568
SspI AATATT 2 cut(s) 782, 1471
SspMI CTAG 6 cut(s) 44, 324, 932, 1191, 1247, 1622
StyI CCWWGG 3 cut(s) 755, 931, 1356
TaaI ACNGT 4 cut(s) 829, 1088, 1264, 1437
TaiI ACGT 1 cut(s) 270
TaqI TCGA 5 cut(s) 125, 132, 585, 679, 1562
TauI GCSGC 2 cut(s) 63, 1457
Tru1I TTAA 7 cut(s) 147, 557, 692, 1062, 1430, 1553, 1690
Tru9I TTAA 7 cut(s) 147, 557, 692, 1062, 1430, 1553, 1690
TscAI CASTG 3 cut(s) 1315, 1440, 1653
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 1229, 1313, 1532, 1651
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 3 cut(s) 849, 1046, 1427
TspRI CASTG 3 cut(s) 1315, 1440, 1653
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 34
VspI ATTAAT 1 cut(s) 1062
XapI RAATTY 1 cut(s) 529
XceI RCATGY 1 cut(s) 436
XmaJI CCTAGG 1 cut(s) 931
XmiI GTMKAC 2 cut(s) 234, 831
XmnI GAANNNNTTC 2 cut(s) 548, 1126
XspI CTAG 6 cut(s) 44, 324, 932, 1191, 1247, 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.