Rh5DG074800

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008848
Physical Location & Seq
Forward (+)
6558884 .. 6564435
5552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG074800.1

Sequence Viewer

Length: 1614 bp
ATGGCAAGAGCAGCTTTGTTCATCATTTTTCTGGTTCTCCAATTACTCCATTTGGGAAGCAGCCGGTCTGTTAAAGACTCTAATCTGCTCATATCCGATGGCTTAGACGACAAGTTGATCAGCCAGTCTTCTTCACTCCTACAAGTAGTGGATGATCTTAAAGCAGATACAGTGACTTGCGAACCCATTTATGGCTTCTTGCCTTGTGCAACTTCAGTCTGGGGGCTGGCTTTCATGGTAATCGTCTATGAGATTTTGCTCTACCTTGCAAATAGCTGTATCACAAGTGGATCTGAACTCTTCTTCAAAATGTATGGGACCGGATTCTTTGGTGCTAGTGTCTTCCACTTGCTCGGCACAATACCACAAGTGGGAATTGTTGTTGTGACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAACATTGGCATGGTTGCAGGGTCAGCAATCATGCTTCTTACATTAGTCTGGGGTTCTGTTGTTGCATTTGGCAGCTATGACATCTCCAAGTCTCCAACTTCTTCAAATCGAGGACATAAGAAGCGCTTCAGTATCACTGGTTATGTAACAACTGATGCTGAAACCAGCTGTACCGCAAGAATCATGATGGTGTCATTGATCCCCTTTCTTATTCTTCAAGTGGCTAAAGTCCTCAATTCAACCTCAGGGATTCGTATTGTGATCTTAATTTCTCTACTAGTTTCATTTGCATTCCTTGTCGCTTACTGCATCTATCAGGTCTTTGAGCCATGGATTCAGAACAGGAGACTTGAATTTTTGATGAGTAAATATGTACAGAGAACCTTGTCACCGAGTTTTCTCACTGCAAGCGGCGAGCCTAATGTATCAAATATACTAGAGGTGTTTTCTAGAATCGACCAGAATCATAATCAGTCTATATCAGCAAAAGAACTGAGAACATTGATCCTAGGAATACAAATAGGGGAAGTAGGTCTGGAGAGTGATTATGTAGAAAAGGTGATGGAGGACTTCGATATATCTGGTGATTCTCAAATATCTGAGCAAGAGTTTGTTAATGGAATCTCAAAATGGGTTAATGCAAGGCCCTCTGTCAATCGTCAAGGTCAAGGAGATAGAAGGTTTTTAAGCAGCAAGTCCGAGGAAGCTTTGGAAGGGCAGCAGAAGCAGGTGGCTAGAAAAAAGAAGAGTAAGCTTACCGATAAATCTTGGACAAATCAAATCAAGGCTGCTTGTCTCTTGATCCTCGGTATTGGCATTATGATTTTGCTTGCAAATCCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTCACCTCTTATGTTTTGATACCCTTGGCTATCAACTATGGACTGGCATTGCAAATAATAACTTCTGCTCAAGATAAGACTGAGAATGCCATCTCTTTAGCACTTTCAGAGATTTATAACAGCGTGTTCATGAACAACGTTATGGGATTGACCATCTTCTTGGCACTTGTTTACATACGTAATTTATCTTGGGATGTCTCTGCATCAGTTTTAGTTGTTCTGATCATTTGCTCAAGTGTGTCTTCTCTCTGCTGGGCTTTTTCTTGCTGCTTCTCTGGGTTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

537

Amino Acids

58.78

Weight (kDa)

5.74

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 289 - 353 4.4e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
AarI CACCTGC 1 cut(s) 1153
Acc36I ACCTGC 1 cut(s) 1153
AciI CCGC 2 cut(s) 609, 846
AclI AACGTT 1 cut(s) 1464
AclWI GGATC 4 cut(s) 298, 628, 934, 1231
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 2 cut(s) 198, 547
AfaI GTAC 2 cut(s) 607, 810
AfeI AGCGCT 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 191, 371
AgsI TTSAA 5 cut(s) 307, 540, 653, 675, 788
AhlI ACTAGT 1 cut(s) 712
AluBI AGCT 6 cut(s) 14, 276, 510, 603, 1142, 1189
AluI AGCT 6 cut(s) 14, 276, 510, 603, 1142, 1189
Alw26I GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
AlwI GGATC 4 cut(s) 298, 628, 934, 1231
AlwNI CAGNNNCTG 2 cut(s) 404, 413
Aor51HI AGCGCT 1 cut(s) 560
AoxI GGCC 1 cut(s) 1079
ApeKI GCWGC 8 cut(s) 11, 60, 507, 1125, 1153, 1223, 1307, 1593
ApoI RAATTY 1 cut(s) 788
Asp700I GAANNNNTTC 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 943
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 318, 1080
AsuHPI GGTGA 4 cut(s) 816, 1006, 1031, 1321
AvaII GGWCC 1 cut(s) 318
AvrII CCTAGG 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 679
BbsI GAAGAC 3 cut(s) 120, 334, 1560
BbvI GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BccI CCATC 5 cut(s) 92, 616, 991, 1424, 1487
BclI TGATCA 2 cut(s) 117, 1548
BcoDI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BcuI ACTAGT 1 cut(s) 712
BfaI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
BfmI CTRYAG 2 cut(s) 405, 414
BfoI RGCGCY 1 cut(s) 562
BfuAI ACCTGC 1 cut(s) 1153
BisI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
BlnI CCTAGG 1 cut(s) 943
BlsI GCNGC 9 cut(s) 13, 62, 509, 848, 1127, 1155, 1225, 1309, 1595
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 318, 1080
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 4 cut(s) 580, 753, 1323, 1538
BpiI GAAGAC 3 cut(s) 120, 334, 1560
BpmI CTGGAG 2 cut(s) 411, 992
BpuEI CTTGAG 2 cut(s) 1380, 1543
BsaAI YACGTR 1 cut(s) 1505
BsaJI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 371
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 4 cut(s) 124, 394, 577, 1374
Bse21I CCTNAGG 1 cut(s) 679
Bse3DI GCAATG 2 cut(s) 429, 1373
BseDI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BseGI GGATG 3 cut(s) 157, 1314, 1525
BseLI CCNNNNNNNGG 2 cut(s) 191, 371
BseMI GCAATG 2 cut(s) 429, 1373
BseMII CTCAG 4 cut(s) 693, 920, 1026, 1398
BseNI ACTGG 4 cut(s) 124, 394, 577, 1374
BseXI GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BseYI CCCAGC 1 cut(s) 1578
BshFI GGCC 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 64, 321
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 191, 371
BsmAI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 2 cut(s) 725, 1417
BsnI GGCC 1 cut(s) 1081
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Bsp19I CCATGG 1 cut(s) 764
BspACI CCGC 2 cut(s) 609, 846
BspANI GGCC 1 cut(s) 1081
BspCNI CTCAG 4 cut(s) 692, 921, 1027, 1399
BspHI TCATGA 2 cut(s) 618, 1455
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 2 cut(s) 409, 418
BspMI ACCTGC 1 cut(s) 1153
BspPI GGATC 4 cut(s) 298, 628, 934, 1231
BsrDI GCAATG 2 cut(s) 429, 1373
BsrFI RCCGGY 1 cut(s) 63
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 4 cut(s) 124, 394, 577, 1374
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 5 cut(s) 764, 943, 1134, 1240, 1350
BssMI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
BssT1I CCWWGG 3 cut(s) 764, 943, 1350
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 305, 1175
BstAPI GCANNNNNTGC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 1505
BstC8I GCNNGC 5 cut(s) 228, 844, 851, 1266, 1312
BstDEI CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
BstDSI CCRYGG 1 cut(s) 764
BstF5I GGATG 3 cut(s) 157, 1314, 1525
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 8 cut(s) 120, 157, 293, 636, 699, 942, 1239, 1551
BstMAI GTCTC 5 cut(s) 531, 775, 1235, 1277, 1528
BstMBI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
BstMWI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
BstSFI CTRYAG 2 cut(s) 405, 414
BstSNI TACGTA 1 cut(s) 1505
BstV1I GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
BstV2I GAAGAC 3 cut(s) 120, 334, 1560
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 1486
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 679
BsuRI GGCC 1 cut(s) 1081
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 3 cut(s) 157, 1314, 1525
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 3 cut(s) 177, 570, 837
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 5 cut(s) 228, 844, 851, 1266, 1312
CaiI CAGNNNCTG 2 cut(s) 404, 413
CciI TCATGA 2 cut(s) 618, 1455
CfoI GCGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 318, 1080
Csp6I GTAC 2 cut(s) 606, 809
CviAII CATG 7 cut(s) 235, 445, 466, 619, 765, 1279, 1456
CviQI GTAC 2 cut(s) 606, 809
DdeI CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
DpnI GATC 8 cut(s) 119, 156, 292, 635, 698, 941, 1238, 1550
DpnII GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Eam1104I CTCTTC 2 cut(s) 305, 1175
EarI CTCTTC 2 cut(s) 305, 1175
Eco105I TACGTA 1 cut(s) 1505
Eco130I CCWWGG 3 cut(s) 764, 943, 1350
Eco47I GGWCC 1 cut(s) 318
Eco47III AGCGCT 1 cut(s) 560
Eco57I CTGAAG 2 cut(s) 198, 547
Eco81I CCTNAGG 1 cut(s) 679
EcoO109I RGGNCCY 1 cut(s) 1080
EcoT14I CCWWGG 3 cut(s) 764, 943, 1350
ErhI CCWWGG 3 cut(s) 764, 943, 1350
FaeI CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
FalI AAGNNNNNCTT 7 cut(s) 30, 545, 577, 1303, 1335, 1552, 1584
FaqI GGGAC 1 cut(s) 331
FatI CATG 7 cut(s) 234, 444, 465, 618, 764, 1278, 1455
FbaI TGATCA 2 cut(s) 117, 1548
Fnu4HI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
FokI GGATG 3 cut(s) 164, 1301, 1532
Fsp4HI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
FspBI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
GlaI GCGC 1 cut(s) 560
GluI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
GsaI CCCAGC 1 cut(s) 1582
GsuI CTGGAG 2 cut(s) 411, 992
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 1 cut(s) 1081
HapII CCGG 2 cut(s) 64, 321
HhaI GCGC 1 cut(s) 561
Hin1II CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HindIII AAGCTT 2 cut(s) 1140, 1187
HinfI GANTC 9 cut(s) 77, 324, 615, 685, 769, 888, 898, 1022, 1056
HpaII CCGG 2 cut(s) 64, 321
HphI GGTGA 4 cut(s) 816, 1006, 1031, 1321
Hpy166II GTNNAC 1 cut(s) 1498
Hpy188I TCNGA 7 cut(s) 97, 295, 774, 1036, 1135, 1435, 1548
Hpy188III TCNNGA 6 cut(s) 619, 885, 971, 1234, 1397, 1456
Hpy8I GTNNAC 1 cut(s) 1498
HpyAV CCTTC 3 cut(s) 1107, 1142, 1329
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 1464, 1504
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
HpyF3I CTNAG 5 cut(s) 103, 679, 929, 1035, 1407
HpySE526I ACGT 2 cut(s) 1464, 1504
Hsp92II CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
HspAI GCGC 1 cut(s) 559
Ksp22I TGATCA 2 cut(s) 117, 1548
Kzo9I GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Lsp1109I GCAGC 8 cut(s) 23, 72, 519, 1137, 1165, 1210, 1294, 1580
LweI GCATC 4 cut(s) 580, 753, 1323, 1538
MaeI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
MaeII ACGT 2 cut(s) 1464, 1504
MaeIII GTNAC 5 cut(s) 172, 385, 394, 580, 822
MalI GATC 8 cut(s) 119, 156, 292, 635, 698, 941, 1238, 1550
MboI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
MflI RGATCY 1 cut(s) 290
MluCI AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
MslI CAYNNNNRTG 2 cut(s) 443, 623
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 2 cut(s) 64, 321
Mva1269I GAATGC 2 cut(s) 725, 1417
MwoI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1159
NcoI CCATGG 1 cut(s) 764
NdeII GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
NlaIII CATG 7 cut(s) 238, 448, 469, 622, 768, 1282, 1459
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 3 cut(s) 172, 385, 822
PagI TCATGA 2 cut(s) 618, 1455
PaqCI CACCTGC 1 cut(s) 1153
PctI GAATGC 2 cut(s) 725, 1417
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 8 cut(s) 324, 615, 685, 769, 888, 898, 1022, 1056
PkrI GCNGC 9 cut(s) 13, 62, 509, 848, 1127, 1155, 1225, 1309, 1595
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 1505
PsiI TTATAA 1 cut(s) 1443
Psp1406I AACGTT 1 cut(s) 1464
PspFI CCCAGC 1 cut(s) 1578
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 318, 1080
PstI CTGCAG 2 cut(s) 409, 418
PstNI CAGNNNCTG 2 cut(s) 404, 413
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 603
RsaI GTAC 2 cut(s) 607, 810
RsaNI GTAC 2 cut(s) 606, 809
RseI CAYNNNNRTG 2 cut(s) 443, 623
SaqAI TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
SatI GCNGC 9 cut(s) 12, 61, 508, 847, 1126, 1154, 1224, 1308, 1594
Sau3AI GATC 8 cut(s) 117, 154, 290, 633, 696, 939, 1236, 1548
Sau96I GGNCC 2 cut(s) 318, 1080
SchI GAGTC 1 cut(s) 71
SfaNI GCATC 4 cut(s) 580, 753, 1323, 1538
SfcI CTRYAG 2 cut(s) 405, 414
SinI GGWCC 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 443, 623
SmlI CTYRAG 2 cut(s) 1395, 1558
SmoI CTYRAG 2 cut(s) 1395, 1558
SnaBI TACGTA 1 cut(s) 1505
SpeI ACTAGT 1 cut(s) 712
Sse9I AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
SsiI CCGC 2 cut(s) 609, 846
SspMI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
StyI CCWWGG 3 cut(s) 764, 943, 1350
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 1467, 1507
TaqI TCGA 3 cut(s) 544, 891, 1008
TasI AATT 6 cut(s) 41, 375, 670, 702, 788, 1507
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 8 cut(s) 324, 615, 685, 769, 888, 898, 1022, 1056
Tru1I TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
Tru9I TTAA 6 cut(s) 72, 159, 701, 1050, 1071, 1121
TscAI CASTG 3 cut(s) 177, 577, 844
TseFI GTSAC 3 cut(s) 172, 385, 822
TseI GCWGC 8 cut(s) 11, 60, 507, 1125, 1153, 1223, 1307, 1593
Tsp45I GTSAC 3 cut(s) 172, 385, 822
TspDTI ATGAA 7 cut(s) 10, 223, 449, 708, 1311, 1444, 1472
TspRI CASTG 3 cut(s) 177, 577, 844
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 788
XbaI TCTAGA 1 cut(s) 884
XmaJI CCTAGG 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 6 cut(s) 336, 713, 872, 885, 944, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.