MD10G1284800.v1.1

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008848
Physical Location & Seq
Reverse (-)
37474783 .. 37476822
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1284800.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGCAAAAATGAACATGAGCATGCTTGCAGGATCAACAATCATGATTCTTACCCTAATATGGGGTTCTGTTGTTGCCTTCGGTAGCTCCGACCTCTCGGATTCTCAAACTTCATCAAATTCAGAAAACAAGAACCCCTTCAGTTTAACTGGTTCTGGTGTAAGAACTGATGTCGAAACCCGAGATACTGCAAGAATCATGATGCTCTCCTCGATCCCATATCTCTTTCTTCAACTGGCAAAAATCTTGACTTCAACCACAGCAGTTCGAGTTGTGATCTTAATTTCTCTACTGATTGCATCTGCAATCCTTGCCACTTACATCATCTACCAGTTCTTTCAACCATGGATTCAGAGCAGAAGGCTTGAATATTTGATGCTTAAATACATACGGAAGATACTGCCGAGTCTTCTGACAGTAGACGGAAATGCTAATGAATCCAGTATAAAAGAGCTGTTCCATAGAGTAGACAAGAATCATAACACATACATATCAGCAGATGAACTTAAAGCATTGATCCTAGGAATACAAATAGAAGAAATAGGTCTGGATGATGATGATTATGCAGAAGAAGTGATGAAGCAATTTGATCTCTCTGGCGACGCTCAAATAACTGAGACGGAGTTTGTGAATGGAATCTCTAAATGGATTAATCCAGATAAGACCCCTGCCAAGAATGAGACATCTATGGAAGATCAGCAGATACCGCTGATCGTTAAGAAAAAGAAGACTAGGGGTGCTGATACATCATGGATGAATCTCCTGAAGGCTGCTTTTCTAATTATTCTAGGAACTGCCATAACAGTTTTTCTTTCAATGCCCCTCATGGTAAGTCTCCAAGAGTTTTCCACCGCTGCAAGCATCCCTTCTTTCATGGCCTCGTATGTTGTGATACCCTTGGCTACAAATTACAGACTGGCACTAATGTCTGTATTGTCTGCCAAAGACAAGACGGAGAATGCCATCTCTTTAACACTTTCTGAGATTTACAACGCGGCATTCATGAACAATATTGTGGGGTTGATCATATTTTTGGCTCTTGTTTACATACGCAATTTATCATGGGATGTCGCTGCTGAAGTTTTAGTTGTTTTAATTATTTCGATAGCGGTTGGTCTTTCTGCAAGCTTCAGCAGAAAGTTCCCATTTTGGACAAGCATTCTAGCATACATTCTGTACCCCATATCACTGCTGCTGGTTTATGTTCTTACCATTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

411

Amino Acids

45.54

Weight (kDa)

5.43

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 146 - 213 1.2e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 420, 468
AccII CGCG 1 cut(s) 995
AciI CCGC 4 cut(s) 707, 852, 995, 1109
AclWI GGATC 3 cut(s) 40, 208, 511
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 4 cut(s) 124, 785, 1098, 1114
AfaI GTAC 1 cut(s) 1178
AfiI CCNNNNNNNGG 2 cut(s) 60, 61
AgsI TTSAA 5 cut(s) 233, 255, 341, 368, 816
AluBI AGCT 3 cut(s) 87, 454, 1128
AluI AGCT 3 cut(s) 87, 454, 1128
Alw26I GTCTC 3 cut(s) 611, 674, 839
AlwI GGATC 3 cut(s) 40, 208, 511
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 1 cut(s) 876
ApeKI GCWGC 4 cut(s) 770, 854, 1073, 1192
ApoI RAATTY 1 cut(s) 118
AseI ATTAAT 1 cut(s) 651
Asp700I GAANNNNTTC 1 cut(s) 137
AspA2I CCTAGG 1 cut(s) 520
AvaI CYCGRG 1 cut(s) 180
AvrII CCTAGG 1 cut(s) 520
BbsI GAAGAC 2 cut(s) 401, 734
BbvI GCAGC 4 cut(s) 757, 841, 1060, 1179
BccI CCATC 2 cut(s) 971, 1218
BclI TGATCA 1 cut(s) 1023
BcoDI GTCTC 3 cut(s) 611, 674, 839
BfaI CTAG 4 cut(s) 521, 732, 788, 1163
BisI GCNGC 5 cut(s) 771, 855, 996, 1074, 1193
BlnI CCTAGG 1 cut(s) 520
BlsI GCNGC 5 cut(s) 772, 856, 997, 1075, 1194
BmeT110I CYCGRG 1 cut(s) 180
BmsI GCATC 4 cut(s) 192, 308, 366, 870
BpiI GAAGAC 2 cut(s) 401, 734
BsaJI CCNNGG 3 cut(s) 344, 520, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 61
Bse1I ACTGG 5 cut(s) 154, 240, 331, 441, 921
BseDI CCNNGG 3 cut(s) 344, 520, 897
BseGI GGATG 5 cut(s) 556, 759, 861, 1072, 1229
BseLI CCNNNNNNNGG 2 cut(s) 60, 61
BseMII CTCAG 2 cut(s) 606, 972
BseNI ACTGG 5 cut(s) 154, 240, 331, 441, 921
BseRI GAGGAG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 757, 841, 1060, 1179
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 1 cut(s) 878
BsiHKCI CYCGRG 1 cut(s) 180
BslI CCNNNNNNNGG 2 cut(s) 60, 61
BsmAI GTCTC 3 cut(s) 611, 674, 839
BsmBI CGTCTC 1 cut(s) 611
BsmI GAATGC 3 cut(s) 964, 998, 1158
BsnI GGCC 1 cut(s) 878
BsoBI CYCGRG 1 cut(s) 180
Bsp143I GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
Bsp19I CCATGG 1 cut(s) 344
BspACI CCGC 4 cut(s) 707, 852, 995, 1109
BspANI GGCC 1 cut(s) 878
BspCNI CTCAG 2 cut(s) 607, 973
BspFNI CGCG 1 cut(s) 995
BspHI TCATGA 3 cut(s) 42, 198, 1002
BspPI GGATC 3 cut(s) 40, 208, 511
BsrI ACTGG 5 cut(s) 154, 240, 331, 441, 921
BssECI CCNNGG 3 cut(s) 344, 520, 897
BssMI GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
BssT1I CCWWGG 3 cut(s) 344, 520, 897
Bst4CI ACNGT 2 cut(s) 418, 805
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 4 cut(s) 23, 27, 859, 1126
BstDEI CTNAG 2 cut(s) 615, 981
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 5 cut(s) 556, 759, 861, 1072, 1229
BstFNI CGCG 1 cut(s) 995
BstKTI GATC 8 cut(s) 35, 216, 279, 519, 592, 697, 714, 1026
BstMAI GTCTC 3 cut(s) 611, 674, 839
BstMBI GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
BstMWI GCNNNNNNNGC 2 cut(s) 311, 706
BstNSI RCATGY 1 cut(s) 25
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 4 cut(s) 757, 841, 1060, 1179
BstV2I GAAGAC 2 cut(s) 401, 734
BsuRI GGCC 1 cut(s) 878
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 5 cut(s) 556, 759, 861, 1072, 1229
BtsI GCAGTG 1 cut(s) 1187
BtsIMutI CAGTG 1 cut(s) 1187
Cac8I GCNNGC 4 cut(s) 23, 27, 859, 1126
CciI TCATGA 3 cut(s) 42, 198, 1002
CseI GACGC 1 cut(s) 611
Csp6I GTAC 1 cut(s) 1177
CviJI RGCY 8 cut(s) 87, 364, 454, 770, 878, 902, 1037, 1128
CviKI_1 RGCY 8 cut(s) 87, 364, 454, 770, 878, 902, 1037, 1128
CviQI GTAC 1 cut(s) 1177
DdeI CTNAG 2 cut(s) 615, 981
DpnI GATC 8 cut(s) 34, 215, 278, 518, 591, 696, 713, 1025
DpnII GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
Eco130I CCWWGG 3 cut(s) 344, 520, 897
Eco57I CTGAAG 4 cut(s) 124, 785, 1098, 1114
Eco88I CYCGRG 1 cut(s) 180
EcoT14I CCWWGG 3 cut(s) 344, 520, 897
ErhI CCWWGG 3 cut(s) 344, 520, 897
Esp3I CGTCTC 1 cut(s) 611
FalI AAGNNNNNCTT 4 cut(s) 122, 154, 850, 882
FbaI TGATCA 1 cut(s) 1023
FblI GTMKAC 2 cut(s) 420, 468
Fnu4HI GCNGC 5 cut(s) 771, 855, 996, 1074, 1193
FokI GGATG 4 cut(s) 563, 766, 848, 1079
Fsp4HI GCNGC 5 cut(s) 771, 855, 996, 1074, 1193
FspBI CTAG 4 cut(s) 521, 732, 788, 1163
GluI GCNGC 5 cut(s) 771, 855, 996, 1074, 1193
HaeIII GGCC 1 cut(s) 878
HgaI GACGC 1 cut(s) 611
HindIII AAGCTT 1 cut(s) 1126
HinfI GANTC 9 cut(s) 46, 101, 195, 349, 406, 437, 475, 636, 757
Hpy166II GTNNAC 3 cut(s) 421, 469, 1045
Hpy188I TCNGA 7 cut(s) 91, 100, 124, 354, 414, 982, 1223
Hpy188III TCNNGA 7 cut(s) 43, 199, 247, 548, 656, 763, 1003
Hpy8I GTNNAC 3 cut(s) 421, 469, 1045
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 5 cut(s) 88, 148, 354, 760, 876
HpyCH4III ACNGT 2 cut(s) 418, 805
HpyCH4V TGCA 7 cut(s) 29, 191, 299, 305, 566, 857, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 706
HpyF3I CTNAG 2 cut(s) 615, 981
Ksp22I TGATCA 1 cut(s) 1023
Kzo9I GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 4 cut(s) 757, 841, 1060, 1179
LweI GCATC 4 cut(s) 192, 308, 366, 870
MaeI CTAG 4 cut(s) 521, 732, 788, 1163
MalI GATC 8 cut(s) 34, 215, 278, 518, 591, 696, 713, 1025
MboI GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
MboII GAAGA 7 cut(s) 221, 401, 406, 548, 581, 704, 739
MluCI AATT 7 cut(s) 118, 282, 584, 780, 907, 1054, 1095
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 104, 220, 833, 889
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 9 cut(s) 146, 281, 381, 507, 651, 717, 971, 1094, 1231
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 2 cut(s) 709, 854
Mva1269I GAATGC 3 cut(s) 964, 998, 1158
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 2 cut(s) 311, 706
NcoI CCATGG 1 cut(s) 344
NdeII GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
NmeAIII GCCGAG 1 cut(s) 429
NspI RCATGY 1 cut(s) 25
PaeI GCATGC 1 cut(s) 25
PagI TCATGA 3 cut(s) 42, 198, 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 3 cut(s) 964, 998, 1158
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 8 cut(s) 46, 101, 195, 349, 437, 475, 636, 757
PkrI GCNGC 5 cut(s) 772, 856, 997, 1075, 1194
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
PshBI ATTAAT 1 cut(s) 651
RsaI GTAC 1 cut(s) 1178
RsaNI GTAC 1 cut(s) 1177
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 9 cut(s) 146, 281, 381, 507, 651, 717, 971, 1094, 1231
SatI GCNGC 5 cut(s) 771, 855, 996, 1074, 1193
Sau3AI GATC 8 cut(s) 32, 213, 276, 516, 589, 694, 711, 1023
SchI GAGTC 1 cut(s) 415
SetI ASST 5 cut(s) 89, 96, 456, 547, 1130
SfaNI GCATC 4 cut(s) 192, 308, 366, 870
SmiMI CAYNNNNRTG 1 cut(s) 20
SphI GCATGC 1 cut(s) 25
Sse9I AATT 7 cut(s) 118, 282, 584, 780, 907, 1054, 1095
SsiI CCGC 4 cut(s) 707, 852, 995, 1109
SspI AATATT 2 cut(s) 371, 1012
SspMI CTAG 4 cut(s) 521, 732, 788, 1163
StyI CCWWGG 3 cut(s) 344, 520, 897
TaaI ACNGT 2 cut(s) 418, 805
TaqI TCGA 4 cut(s) 174, 212, 268, 1103
TasI AATT 7 cut(s) 118, 282, 584, 780, 907, 1054, 1095
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 8 cut(s) 46, 101, 195, 349, 437, 475, 636, 757
Tru1I TTAA 9 cut(s) 146, 281, 381, 507, 651, 717, 971, 1094, 1231
Tru9I TTAA 9 cut(s) 146, 281, 381, 507, 651, 717, 971, 1094, 1231
TscAI CASTG 1 cut(s) 1194
TseI GCWGC 4 cut(s) 770, 854, 1073, 1192
TspDTI ATGAA 9 cut(s) 26, 102, 450, 516, 593, 770, 862, 991, 1019
TspGWI ACGGA 4 cut(s) 406, 438, 635, 968
TspRI CASTG 1 cut(s) 1194
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 25
XmaJI CCTAGG 1 cut(s) 520
XmiI GTMKAC 2 cut(s) 420, 468
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 4 cut(s) 521, 732, 788, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.