MD05G1305500.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008848
Physical Location & Seq
Reverse (-)
43769331 .. 43772360
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1305500.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGTCTAGACAAGCTTTGTTCATCATTTTCCTGGTCCTCCAACTAGTCCATTTGGGAAGTGGCCGGATTGTTAGAGACTCCGGTCTTATATCCGATGGAATAAACGACGTAATAAGCCAATCTTCGATCCTCGAAGCACTGAATCTTAAAGCAGATTCTGTGACTTGTGAACCAACTTATGGCTTCTTGCCTTGTGCAACTTCAGTTTGGGGGCTGCTTTCCCTGATAATCGTCTACGAGGTCTTGCTCTCCCTTGCAGATCAGTATGTTTCAAAAGGATCTGAATTCTTTTTTGAGTTGTTTGGGACTGGTATCTTTGGTGCTAGTGCTTTCCACATGCTTGGCTTGATTCCACAAGTTGGAATGGTTCTTGTAACCGGAGTTACAGCAACTACAACAACTGTGGAGGAAATGGCAGAAATGAACATGGGCATGCTTGCAGGATCAACAATCATGATTCTTACCCTAATTTGGGGTTCTGTTGTTGCTTTCGGCAGCTACGACCTCTCGGATTCTCAAAGTTCATCAAATCCAGAAACTAAGAAACCCTTCAGTTTAACTGGGTCTGGTGTCAGAACTGATGTCGAAACCCGAGATACTGCAAGAATCATGATGCTCTCGTTGATCCCATTTCTCATTCTTCAACTGGCAAAAATCTTGACTTCAACCAGAGCAGTTCGAGTTGTGATCTTAGTTTCTTTACTGATTGCATCTGCATTCCTTGCCAATTACTTCATCTACCAGTTCTTTCAGCCATGGATTCAGAGCAGAAGGCATGAATATTTGATGCGTAAATACAGACTGGAAAACATACTGCCAAGTCTTCTGACAGTACGCGGAAACCCTAATGTATCCAATATAAAAGGGCTGTTCCATAGAATAGACAAGAATCATAACACATACATATCAGCAGATGAACTTAGAGCATTGATCCTAGGAATACAAATAGAAGAATTTGGTCTGGATGACGATGATTATGTAGCAGAAGTGATGAAGCAATTTGATATCTCTGGCGACGCTCAAATAGCTGAGACGGAATTTGTGAATGGAATCTCAAAATGGATTGATCCAGTTATGCCCTCTGCCAACAGTAAAGGTCATGAACATAAATCGCTTTTCAGAAGAAATAATTCAAAGAATGAGAAAACTATGGAAGATCAGCAGACGCCGCTGATCTCTAAGAAAAAGAAGACTAAGGGTGCTGATAAATCGTGGATGAATCTCCTGAAGGCTGTTTTTCTAATTATTCTAGGAACTGCCATGACGGTCTTTCTTTCAATGCCGCTCATGGTAAGTCTCCAAGAGTTCTCCACAGCTGCAAGCATCCCTTCTTTCGTGGTCTCTTATGTTGTGATACCCTTGTCTACGAACTACAGACTGGCACTAACGTCAGTAACGTCTGCCAGAGACAGAACGGAGAATGCAATTTCTTTAACACTTTCTGAGATTTACAACGCGGTGTTCATGAACAACATTTTGGGGTTGGTCATATTTTTGGCCCTTGTTTACATACGCAATTTATCATGGGACGTCTCTGCTGAAGTTTTAGTTGTTTTACTTATTTCGACAGCGGTTGGTCTTTCTGCCAGCTTCAGCAGAAAGTTCCCATTTTGGACAAGCATTCTAGCATACACTCTGTACCCCCTATCACTGCTTCTGCTTTATGTTCTTGTCGCTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

565

Amino Acids

62.5

Weight (kDa)

6.21

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 286 - 351 6.6e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1533
AccB7I CCANNNNNTGG 2 cut(s) 179, 359
AccBSI CCGCTC 1 cut(s) 1285
AccI GTMKAC 2 cut(s) 234, 1364
AccII CGCG 2 cut(s) 837, 1457
AciI CCGC 5 cut(s) 837, 1169, 1283, 1457, 1571
AclWI GGATC 6 cut(s) 121, 286, 451, 619, 925, 1061
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 3 cut(s) 284, 953, 1037
AcuI CTGAAG 5 cut(s) 186, 535, 1247, 1560, 1576
AcyI GRCGYC 2 cut(s) 1166, 1530
AfaI GTAC 2 cut(s) 834, 1640
AfiI CCNNNNNNNGG 4 cut(s) 179, 359, 471, 472
AgsI TTSAA 5 cut(s) 273, 644, 666, 1134, 1278
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 1460, 1492
AluBI AGCT 5 cut(s) 14, 498, 1028, 1316, 1590
AluI AGCT 5 cut(s) 14, 498, 1028, 1316, 1590
Alw26I GTCTC 6 cut(s) 69, 1025, 1301, 1345, 1401, 1537
AlwI GGATC 6 cut(s) 121, 286, 451, 619, 925, 1061
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 2 cut(s) 61, 1497
ApeKI GCWGC 3 cut(s) 214, 495, 1316
ApoI RAATTY 3 cut(s) 284, 953, 1037
Asp700I GAANNNNTTC 2 cut(s) 548, 1129
AspA2I CCTAGG 1 cut(s) 934
AspS9I GGNCC 2 cut(s) 34, 1498
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 1 cut(s) 34
AvrII CCTAGG 1 cut(s) 934
BaeI ACNNNNGTAYC 2 cut(s) 1346, 1379
BbsI GAAGAC 2 cut(s) 815, 1196
BbvI GCAGC 3 cut(s) 201, 507, 1303
BccI CCATC 2 cut(s) 89, 1680
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 1 cut(s) 862
BcoDI GTCTC 6 cut(s) 69, 1025, 1301, 1345, 1401, 1537
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 6 cut(s) 6, 44, 324, 935, 1250, 1625
BfmI CTRYAG 1 cut(s) 1372
BfuI GTATCC 1 cut(s) 862
BisI GCNGC 5 cut(s) 215, 496, 1169, 1283, 1317
BlnI CCTAGG 1 cut(s) 934
BlsI GCNGC 5 cut(s) 216, 497, 1170, 1284, 1318
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 2 cut(s) 34, 1498
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 4 cut(s) 603, 719, 777, 1332
BmuI ACTGGG 1 cut(s) 570
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 815, 1196
BsaHI GRCGYC 2 cut(s) 1166, 1530
BsaI GGTCTC 1 cut(s) 1345
BsaJI CCNNGG 2 cut(s) 755, 934
BsaWI WCCGGW 2 cut(s) 80, 377
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 359, 471, 472
Bse1I ACTGG 7 cut(s) 313, 565, 651, 742, 807, 1070, 1383
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 755, 934
BseGI GGATG 4 cut(s) 970, 1221, 1323, 1691
BseLI CCNNNNNNNGG 4 cut(s) 179, 359, 471, 472
BseMII CTCAG 2 cut(s) 1020, 1434
BseNI ACTGG 7 cut(s) 313, 565, 651, 742, 807, 1070, 1383
BseXI GCAGC 3 cut(s) 201, 507, 1303
Bsh1236I CGCG 2 cut(s) 837, 1457
BshFI GGCC 2 cut(s) 63, 1499
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 3 cut(s) 64, 81, 378
BslFI GGGAC 2 cut(s) 319, 1541
BslI CCNNNNNNNGG 4 cut(s) 179, 359, 471, 472
BsmAI GTCTC 6 cut(s) 69, 1025, 1301, 1345, 1401, 1537
BsmBI CGTCTC 2 cut(s) 1025, 1537
BsmFI GGGAC 2 cut(s) 319, 1541
BsmI GAATGC 3 cut(s) 716, 1426, 1620
BsnI GGCC 2 cut(s) 63, 1499
Bso31I GGTCTC 1 cut(s) 1345
BsoBI CYCGRG 1 cut(s) 591
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 5 cut(s) 837, 1169, 1283, 1457, 1571
BspANI GGCC 2 cut(s) 63, 1499
BspCNI CTCAG 2 cut(s) 1021, 1435
BspFNI CGCG 2 cut(s) 837, 1457
BspHI TCATGA 4 cut(s) 453, 609, 1099, 1464
BspPI GGATC 6 cut(s) 121, 286, 451, 619, 925, 1061
BspTNI GGTCTC 1 cut(s) 1345
BsrBI CCGCTC 1 cut(s) 1285
BsrI ACTGG 7 cut(s) 313, 565, 651, 742, 807, 1070, 1383
BssECI CCNNGG 2 cut(s) 755, 934
BssNI GRCGYC 2 cut(s) 1166, 1530
BssT1I CCWWGG 2 cut(s) 755, 934
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 4 cut(s) 403, 832, 1091, 1267
BstACI GRCGYC 2 cut(s) 1166, 1530
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 4 cut(s) 434, 438, 1321, 1588
BstDEI CTNAG 7 cut(s) 540, 691, 920, 1029, 1179, 1194, 1443
BstDSI CCRYGG 1 cut(s) 755
BstF5I GGATG 4 cut(s) 970, 1221, 1323, 1691
BstFNI CGCG 2 cut(s) 837, 1457
BstMAI GTCTC 6 cut(s) 69, 1025, 1301, 1345, 1401, 1537
BstMWI GCNNNNNNNGC 3 cut(s) 722, 1025, 1168
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 2 cut(s) 340, 436
BstPAI GACNNNNGTC 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 1372
BstUI CGCG 2 cut(s) 837, 1457
BstV1I GCAGC 3 cut(s) 201, 507, 1303
BstV2I GAAGAC 2 cut(s) 815, 1196
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 278
BsuI GTATCC 1 cut(s) 862
BsuRI GGCC 2 cut(s) 63, 1499
BtgI CCRYGG 1 cut(s) 755
BtsCI GGATG 4 cut(s) 970, 1221, 1323, 1691
BtsI GCAGTG 1 cut(s) 1649
BtsIMutI CAGTG 2 cut(s) 137, 1649
Cac8I GCNNGC 4 cut(s) 434, 438, 1321, 1588
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 4 cut(s) 453, 609, 1099, 1464
Cfr13I GGNCC 2 cut(s) 34, 1498
CseI GACGC 2 cut(s) 1025, 1174
Csp6I GTAC 2 cut(s) 833, 1639
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviQI GTAC 2 cut(s) 833, 1639
DdeI CTNAG 7 cut(s) 540, 691, 920, 1029, 1179, 1194, 1443
EaeI YGGCCR 1 cut(s) 61
Eco130I CCWWGG 2 cut(s) 755, 934
Eco31I GGTCTC 1 cut(s) 1345
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 5 cut(s) 186, 535, 1247, 1560, 1576
Eco88I CYCGRG 1 cut(s) 591
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 30
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 2 cut(s) 755, 934
ErhI CCWWGG 2 cut(s) 755, 934
Esp3I CGTCTC 2 cut(s) 1025, 1537
FalI AAGNNNNNCTT 6 cut(s) 106, 138, 533, 565, 1312, 1344
FaqI GGGAC 2 cut(s) 319, 1541
FblI GTMKAC 2 cut(s) 234, 1364
Fnu4HI GCNGC 5 cut(s) 215, 496, 1169, 1283, 1317
FokI GGATG 3 cut(s) 977, 1228, 1310
Fsp4HI GCNGC 5 cut(s) 215, 496, 1169, 1283, 1317
FspBI CTAG 6 cut(s) 6, 44, 324, 935, 1250, 1625
GluI GCNGC 5 cut(s) 215, 496, 1169, 1283, 1317
HaeIII GGCC 2 cut(s) 63, 1499
HapII CCGG 3 cut(s) 64, 81, 378
HgaI GACGC 2 cut(s) 1025, 1174
Hin1I GRCGYC 2 cut(s) 1166, 1530
HindIII AAGCTT 1 cut(s) 12
HpaII CCGG 3 cut(s) 64, 81, 378
Hpy166II GTNNAC 4 cut(s) 170, 235, 1365, 1507
Hpy188I TCNGA 9 cut(s) 94, 283, 511, 575, 765, 828, 1121, 1444, 1685
Hpy188III TCNNGA 9 cut(s) 6, 454, 533, 610, 658, 962, 1100, 1225, 1465
Hpy8I GTNNAC 4 cut(s) 170, 235, 1365, 1507
Hpy99I CGWCG 2 cut(s) 110, 1019
HpyAV CCTTC 4 cut(s) 559, 765, 1222, 1338
HpyCH4III ACNGT 4 cut(s) 403, 832, 1091, 1267
HpyCH4IV ACGT 4 cut(s) 108, 1388, 1397, 1530
HpyCH4V TGCA 8 cut(s) 197, 257, 440, 602, 710, 716, 1319, 1424
HpyF10VI GCNNNNNNNGC 3 cut(s) 722, 1025, 1168
HpyF3I CTNAG 7 cut(s) 540, 691, 920, 1029, 1179, 1194, 1443
HpySE526I ACGT 4 cut(s) 108, 1388, 1397, 1530
Hsp92I GRCGYC 2 cut(s) 1166, 1530
Lsp1109I GCAGC 3 cut(s) 201, 507, 1303
LweI GCATC 4 cut(s) 603, 719, 777, 1332
MaeI CTAG 6 cut(s) 6, 44, 324, 935, 1250, 1625
MaeII ACGT 4 cut(s) 108, 1388, 1397, 1530
MaeIII GTNAC 4 cut(s) 160, 373, 382, 1393
MbiI CCGCTC 1 cut(s) 1285
MboII GAAGA 7 cut(s) 114, 632, 815, 962, 1134, 1166, 1201
MflI RGATCY 1 cut(s) 278
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 64, 340
MnlI CCTC 6 cut(s) 47, 140, 232, 400, 515, 1090
MroXI GAANNNNTTC 2 cut(s) 548, 1129
MseI TTAA 4 cut(s) 147, 557, 1433, 1693
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 3 cut(s) 1171, 1316, 1571
MspI CCGG 3 cut(s) 64, 81, 378
MspR9I CCNGG 1 cut(s) 32
Mva1269I GAATGC 3 cut(s) 716, 1426, 1620
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 2 cut(s) 837, 1457
MwoI GCNNNNNNNGC 3 cut(s) 722, 1025, 1168
NcoI CCATGG 1 cut(s) 755
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 2 cut(s) 340, 436
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 4 cut(s) 453, 609, 1099, 1464
PcsI WCGNNNNNNNCGW 2 cut(s) 498, 1394
PctI GAATGC 3 cut(s) 716, 1426, 1620
PdmI GAANNNNTTC 2 cut(s) 548, 1129
PflMI CCANNNNNTGG 2 cut(s) 179, 359
PkrI GCNGC 5 cut(s) 216, 497, 1170, 1284, 1318
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PshAI GACNNNNGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspPI GGNCC 2 cut(s) 34, 1498
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 2 cut(s) 834, 1640
RsaNI GTAC 2 cut(s) 833, 1639
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 4 cut(s) 147, 557, 1433, 1693
SatI GCNGC 5 cut(s) 215, 496, 1169, 1283, 1317
Sau96I GGNCC 2 cut(s) 34, 1498
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 32
SfaNI GCATC 4 cut(s) 603, 719, 777, 1332
SfcI CTRYAG 1 cut(s) 1372
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 43
SphI GCATGC 1 cut(s) 436
SsiI CCGC 5 cut(s) 837, 1169, 1283, 1457, 1571
SspI AATATT 1 cut(s) 782
SspMI CTAG 6 cut(s) 6, 44, 324, 935, 1250, 1625
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 755, 934
TaaI ACNGT 4 cut(s) 403, 832, 1091, 1267
TaiI ACGT 4 cut(s) 111, 1391, 1400, 1533
TaqI TCGA 5 cut(s) 125, 132, 585, 679, 1565
TauI GCSGC 2 cut(s) 1171, 1285
Tru1I TTAA 4 cut(s) 147, 557, 1433, 1693
Tru9I TTAA 4 cut(s) 147, 557, 1433, 1693
TscAI CASTG 2 cut(s) 144, 1656
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 3 cut(s) 214, 495, 1316
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 2 cut(s) 1049, 1430
TspRI CASTG 2 cut(s) 144, 1656
Van91I CCANNNNNTGG 2 cut(s) 179, 359
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 3 cut(s) 284, 953, 1037
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 2 cut(s) 340, 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmaJI CCTAGG 1 cut(s) 934
XmiI GTMKAC 2 cut(s) 234, 1364
XmnI GAANNNNTTC 2 cut(s) 548, 1129
XspI CTAG 6 cut(s) 6, 44, 324, 935, 1250, 1625
ZraI GACGTC 1 cut(s) 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.